Method and system for detecting absorbed dose of external irradiation in-vitro cells

Abstract

The invention relates to the technical field of radiobiology, and provides an external irradiation isolated cell absorbed dose detection method and system.The conversion coefficient of the cell layer absorbed dose and the air absorbed dose is calculated in advance through an isolated cell geometric model; only the absorbed dose in the cavity gas at the irradiation position of the cell layer needs to be measured through the ionization chamber, and then the absorbed dose of the cell layer is obtained by utilizing the conversion coefficient of the absorbed dose of the cell layer and the absorbed dose of air; compared with a traditional method, the method has the advantages that the testing efficiency of the absorbed dose of the in-vitro cells is greatly improved, and the testing accuracy is further improved.

Description

Technical Field

[0001] The invention relates to the technical field of radiation biology, and relates to a method and system for detecting external irradiation in vitro cell absorbed dose. Background Art

[0002] Quantitative detection of absorbed dose can not only predict the biological effect of radiation, but also be used in the dose optimization process in the development of radiotherapy drugs. It is of great significance for the optimization of treatment effect, the safety assessment of treatment and the individual adjustment of dose. At present, the quantitative method of cell absorbed dose mainly uses the ionization chamber dose meter to measure the absorbed dose of the cell culture medium in the cell dish, and the absorbed dose value of the cell culture medium is used to approximate the absorbed dose of the cell. The basic process of measuring absorbed dose by ionization chamber is: by measuring the ionization charge of secondary particles generated by ionizing radiation in the process of interaction with matter, the absorbed dose is calculated; that is, the measurement of absorbed dose by ionization chamber can be divided into two steps: first, the ionization charge generated by ionizing radiation is measured, and then the average ionization energy of air is used to calculate and convert it into the energy deposited by ionizing radiation, that is, the absorbed dose. The kinetic energy of electrons consumed by each pair of positive and negative particles in the air is basically a constant for all electrons, that is, the average ionization energy is W / e=33.97J / C.

[0003] However, the disadvantages of using ionization chamber dose meters for quantitative detection of cell absorbed dose are as follows: 1) Due to the influence of factors such as the type of radiation particles, the energy of the particles, the amount of cell culture fluid, and the thickness of the cells in the culture fluid, it can only approximately reflect the actual absorbed dose of the cells and there is a large uncertainty; 2) Since energy response is the key factor affecting the measurement accuracy of the absorbed dose of the cell culture fluid directly measured using an ionization chamber, and the dose calibration procedure is complicated, it is difficult to obtain an accurate dose calibration factor; 3) For neutron external irradiation, the use of an ionization chamber to directly measure the absorbed dose of the cell culture fluid requires the application of multiple correction factors, which leads to a greater difference between the absorbed dose of the cell culture fluid and the actual cell dose value.

[0004] Therefore, there is an urgent need for a scheme for detecting external irradiation in vitro cell absorbed dose with high measurement efficiency. Summary of the invention

[0005] The present invention provides a method and system for detecting external irradiation in vitro cell absorbed dose, so as to solve at least one problem existing in the prior art.

[0006] To achieve the above-mentioned object, in a first aspect, the present invention provides a method for detecting an external irradiation in vitro cell absorbed dose, comprising: obtaining an irradiation particle type and an irradiation distance, and selecting an ionization chamber type and a filling gas type of the ionization chamber according to the irradiation particle type; According to the type of irradiated particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, using the ionization chamber to measure the absorbed dose in the cavity gas at the irradiation position of the cell layer; and determining the absorbed dose in the air according to the absorbed dose in the cavity gas; The cell layer absorbed dose is obtained based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose; the conversion coefficient between the cell layer absorbed dose and the air absorbed dose is obtained through a preset in vitro cell geometric model using a particle transport simulation program.

[0007] Further, preferably, the method of obtaining the conversion coefficient between the cell layer absorbed dose and the air absorbed dose by using a preset in vitro cell geometric model based on a particle transport simulation program includes: Establishing an in vitro cell geometric model; the in vitro cell geometric model includes a cell culture dish, a cell layer, and a cell culture fluid layer; Using a particle transport simulation program, the absorbed dose of the cell layer in the cell culture dish is obtained according to the particle type, particle energy and irradiation distance through an in vitro cell geometric model; the absorbed dose of air is obtained according to the particle type, particle energy and irradiation distance using a particle transport simulation program; According to the absorbed dose of the cell layer in the cell culture dish and the absorbed dose of the air, a conversion coefficient between the absorbed dose of the cell layer and the absorbed dose of the air is obtained.

[0008] Further, preferably, in the process of measuring the absorbed dose in the cavity gas at the irradiation position of the cell layer using the ionization chamber according to the type of irradiation particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, When the irradiated particle type is photon or electron, the type of ionization chamber is graphite cavity ionization chamber; When the filling gas of the graphite cavity ionization chamber is air, the absorbed dose in the cavity gas at the irradiation position of the cell layer measured by the ionization chamber is equal to the absorbed dose in the air; When the filling gas of the graphite cavity ionization chamber is gas g, the air absorption dose D is determined according to the absorption dose in the cavity gas. a Determined by the following formula, D a =D mg× S w,g Among them, D mg is the absorbed dose in the cavity gas g obtained by ionization chamber measurement; S w,g is the average mass collision stopping power ratio of the cavity ionization chamber wall material graphite w and the gas g in the cavity; Sw,g = , (s / ρ) w is the mass collision stopping power of graphite for secondary electrons or electrons for photons; (s / ρ) g It is the mass collision stopping power of the cavity gas for secondary electrons or electrons of photons.

[0009] Further, preferably, in the process of measuring the absorbed dose in the cavity gas at the irradiation position of the cell layer using the ionization chamber according to the type of irradiation particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, When the irradiation particle type is neutron, the type of ionization chamber is tissue equivalent ionization chamber; Determine the air absorbed dose D based on the absorbed dose in the cavity gas a Determined by the following formula, D a =D mg ×(K a / K g ) Among them, D mg K is the absorbed dose in the cavity gas g obtained by measuring the tissue equivalent ionization chamber; a and K g They are the kerma of neutrons in air and in the gas g in the ionization chamber respectively.

[0010] Further, preferably, in the process of measuring the absorbed dose in the cavity gas at the irradiation position of the cell layer using the ionization chamber according to the type of irradiation particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, When the irradiating particles contain both neutrons and photons; The absorbed dose of neutrons in the cavity gas is obtained by using a tissue equivalent ionization chamber; the absorbed dose of photons in the cavity gas is obtained by using a hydrogen-free ionization chamber; The air absorption dose of neutrons is determined according to the absorption dose of neutrons in the cavity gas, and the air absorption dose of photons is determined according to the absorption dose of photons in the cavity gas.

[0011] Further, preferably, the hydrogen-free ionization chamber is a graphite-carbon dioxide ionization chamber or a graphite-argon ionization chamber.

[0012] Further, preferably, in the process of obtaining the cell layer absorbed dose based on the air absorbed dose and the conversion coefficient between the pre-acquired cell layer absorbed dose and the air absorbed dose, When the particle source is neutrons, photons or electrons, the absorbed dose of the cell layer is obtained by the following formula: D cell =D a× R(S,E,F)×A ; Among them, D cell D is the dose absorbed by the cell layer; a is the air absorption dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorption dose and the air absorption dose; A is the emission intensity of the particle source P(S,E,A); When the particle source contains both neutrons and photons, the neutron absorption dose in the cell layer is obtained by the following formula: D cell =D a，N× R(S,E,F)×A ; The photon absorbed dose of the cell layer is obtained by the following formula: D cell =D a，P× R(S,E,F)×A ; Among them, D cell Dose absorption for the cell layer; D a，N Neutron air absorbed dose values ​​obtained for ionization chamber measurements; D a，P is the photon air absorption dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorption dose and the air absorption dose; A is the emission intensity of the particle source P(S,E,A).

[0013] Further, preferably, the cell layer in the in vitro cell geometric model is composed of tissue equivalent material.

[0014] In order to solve the above problems, in a second aspect, the present invention also protects a system for detecting external irradiation in vitro cell absorbed dose, which is used to implement the above-mentioned method for detecting external irradiation in vitro cell absorbed dose. The system comprises: an irradiation data acquisition unit, used to acquire the irradiation particle type and the irradiation distance, and select the type of the ionization chamber and the type of filling gas of the ionization chamber according to the irradiation particle type; A measuring unit, configured to measure the absorbed dose in the cavity gas at the irradiation position of the cell layer by using the ionization chamber according to the type of irradiated particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber; and determine the absorbed dose in the air according to the absorbed dose in the cavity gas; The absorbed dose acquisition unit is used to obtain the cell layer absorbed dose based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose; the conversion coefficient between the cell layer absorbed dose and the air absorbed dose is obtained through a preset in vitro cell geometric model using a particle transport simulation program.

[0015] In order to solve the above problem, the present invention further provides an electronic device according to a third aspect, the electronic device comprising: at least one processor; and, a memory communicatively connected to the at least one processor; wherein, The memory stores instructions that can be executed by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can perform the steps in the above-mentioned external irradiation in vitro cell absorption dose detection method.

[0016] The external irradiation in vitro cell absorption dose detection method, system and electronic device of the present invention have the following beneficial effects: by using a particle transport simulation program, the effects of particle type, energy level, and the thickness of the cell culture fluid and cell layer on the cell absorption dose are comprehensively considered; first, the conversion coefficient between the in vitro cell absorption dose and the air absorption dose under different irradiation conditions is determined. Then, the air absorption dose is directly measured using an ionization chamber, and the measurement results of the air absorption dose are converted into the in vitro cell absorption dose by using the conversion coefficient between the cell layer absorption dose and the air absorption dose obtained previously. Compared with the traditional method of directly using an ionization chamber to measure the cell culture fluid absorption dose, the present invention not only simplifies the measurement process, but also significantly improves the accuracy of the in vitro cell absorption dose measurement. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of a flow chart of a method for detecting external irradiation in vitro cell absorbed dose provided by an embodiment of the present invention; Figure 2 A schematic diagram of the structure of an in vitro cell geometric model provided by an embodiment of the present invention; Figure 3 A schematic diagram of the principle of a system for detecting external irradiation in vitro cell absorbed dose provided by an embodiment of the present invention; Figure 4 A schematic diagram of the internal structure of an electronic device for implementing a method for detecting external irradiation in vitro cell absorbed dose provided by an embodiment of the present invention; The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0018] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.

[0019] Reference Figure 1 FIG. 1 is a flow chart of a method for detecting external irradiation in vitro cell absorbed dose provided by an embodiment of the present invention. The method can be executed by a device, and the device can be implemented by software and / or hardware.

[0020] In this embodiment, the method for detecting external irradiation in vitro cell absorbed dose includes steps S110 to S130.

[0021] S110 , obtaining the type of irradiated particles and the irradiation distance, and selecting the type of ionization chamber and the type of filling gas of the ionization chamber according to the type of irradiated particles.

[0022] It should be noted that the irradiated particle type includes any one of neutrons, photons, and electrons; or the irradiated particle type includes both neutrons and photons.

[0023] When the irradiated particles are photons or electrons, the type of ionization chamber is a graphite cavity ionization chamber. When the irradiated particles are neutrons, the type of ionization chamber is a tissue equivalent ionization chamber. When the irradiated particles contain both neutrons and photons, the absorbed dose of neutrons in the cavity gas is obtained using a tissue equivalent ionization chamber; the absorbed dose of photons in the cavity gas is obtained using a hydrogen-free ionization chamber.

[0024] Specifically, the wall material and cavity gas of the tissue equivalent ionization chamber are designed to be equivalent to the tissue to be tested to ensure the accuracy and reliability of the measurement results. For example, the design of the tissue equivalent ionization chamber makes it similar to human soft tissue in its response to neutrons and gamma radiation, which makes it more accurate in measuring the absorbed dose in human tissue. Specifically, the wall material of the tissue equivalent ionization chamber is tissue equivalent material (usually composed of materials containing different mass percentages of C, H, N, and O atoms), and the gas filled is also tissue equivalent gas, which can be different from the wall material but has the same atomic composition. Therefore, in order to ensure that the measurement results can truly reflect the energy deposition of neutrons in biological tissues under neutron irradiation conditions, the tissue equivalent ionization chamber is selected to measure the absorbed dose of neutrons in cavity gas.

[0025] In a specific embodiment, based on the superior performance in measuring the absorbed dose of photons in the cavity gas, the hydrogen-free ionization chamber is selected as a graphite-carbon dioxide ionization chamber or a graphite-argon ionization chamber. The absorbed dose of photons in the cavity gas refers to the measure of energy absorbed by gas molecules when photons interact with gas molecules. The measurement of the absorbed dose of photons in the cavity gas is crucial for determining the energy distribution and dose distribution of photon beams in treatment or experiments. Specifically, the graphite-carbon dioxide ionization chamber and the graphite-argon ionization chamber have sensitivity and stability to photon beams. These ionization chambers can accurately measure the energy absorption of photons in gas media, thereby providing basic data for the accurate quantification of the absorbed dose of cells in vitro. Through these data, the conversion coefficient of the absorbed dose of the cell layer and the absorbed dose of air obtained by the present invention can be used to accurately calculate the absorbed dose of cells in vitro. The tissue equivalent ionization chamber is often paired with a hydrogen-free ionization chamber that is not very sensitive to neutrons but sensitive to gamma radiation to form a dual ionization chamber detector for measuring the absorbed dose in the cavity gas in the scene of mixed radiation field.

[0026] S120. According to the type of irradiated particles, the type of ionization chamber, the irradiation distance, and the type of filling gas of the ionization chamber, use the ionization chamber to measure the absorbed dose in the cavity gas at the irradiation position of the cell layer; and determine the absorbed dose in the air according to the absorbed dose in the cavity gas.

[0027] S130, obtaining a cell layer absorbed dose based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose; the conversion coefficient between the cell layer absorbed dose and the air absorbed dose is obtained by using a preset in vitro cell geometric model based on a particle transport simulation program.

[0028] Example 1

[0029] The method of obtaining the conversion coefficient between the cell layer absorbed dose and the air absorbed dose by using a particle transport simulation program through a preset in vitro cell geometric model includes: S101. Establishing an in vitro cell geometric model; the in vitro cell geometric model includes a cell culture dish, a cell layer, and a cell culture fluid layer.

[0030] Figure 2 A schematic diagram of the structure of an in vitro cell geometric model provided by an embodiment of the present invention; Figure 2 As shown in the figure, the in vitro cell geometric model includes a cell culture dish, a cell layer, and a cell culture fluid layer; the outer diameter of the cell culture container is R cm, the inner diameter is r cm, the thickness of the cell layer is H1 cm, and the thickness of the cell culture fluid layer is H2 cm; the cell layer medium is composed of tissue equivalent materials. Among them, the density of the cell layer is ρ c g / cm 3 , then the mass of the cell layer Mc =ρ c ×πr 2 ×H1 (g).

[0031] The cell culture medium and the thickness of the cell layer will mainly affect the degree of particle scattering, thereby affecting the absorbed dose of the cell layer.

[0032] S102. Using a particle transport simulation program, the absorbed dose of the cell layer in the cell culture dish is obtained according to the particle type, particle energy and irradiation distance through an in vitro cell geometric model; and using a particle transport simulation program, the absorbed dose of the air is obtained according to the particle type, particle energy and irradiation distance.

[0033] In the first part, the absorbed dose D of the cell layer in the cell culture dish is obtained according to the particle type, particle energy and irradiation distance through the in vitro cell geometry model using a particle transport simulation program. ca (S,E,F).

[0034] Specifically, the properties of the particle source are represented by P(S,E,A), where P represents a particle source; S represents the particle type, E is the energy of the particle source, and A represents the emission intensity of the particle source. The spatial position coordinates of the particle source P(S,E,A) are represented by O(a,b,c), where a,b,c represent the coordinate values ​​of point O in the X, Y, and Z directions of the three-dimensional rectangular coordinate system, respectively. The spatial position of the cell culture dish is represented by N (aˊ,bˊ,cˊ), where aˊ,bˊ,cˊ represent the coordinate values ​​of point N in the X, Y, and Z directions of the three-dimensional rectangular coordinate system, respectively.

[0035] It should be noted that when the particle irradiation source is a rectangular plane source, assuming the length of the rectangle is K cm and the width is Y cm, then X>R, Y>R, X>(H1+H2), Y>(H1+H2); if the particle irradiation source is a circular plane photon source, assuming the diameter of the circle is Rˊcm, then Rˊ>R, Rˊ>(H1+H2).

[0036] Using a particle transport simulation program, calculate the deposition energy E of the particle emitted by the particle source P(S,E,A) located at O(a,b,c) at the cell layer in the cell culture dish at position N (aˊ,bˊ,cˊ). c (S,E,F), where F is the distance between point O(a,b,c) and point P(aˊ,bˊ,cˊ).

[0037] Then, according to the deposition energy E of the cell layer in the cell culture dish at position N (aˊ, bˊ, cˊ), c (S, E, F calculate the absorbed dose D of a single particle in the cell layer c (S,E,F): D c (S,E,F)=E c (S,E,F)×1.602×10 -10 / M c (Gy) .

[0038] Among them, M c is the mass of the cell layer.

[0039] In the second part, the particle transport simulation program is used to obtain the air absorbed dose D according to the particle type, particle energy and irradiation distance. ca (S,E,F).

[0040] Using the particle transport program, calculate the air deposition energy E of the particle emitted by the particle source P(S,E,A) located at O(a,b,c) at the position N (aˊ,bˊ,cˊ) a (S,E,F); where F is the distance between point O(a,b,c) and point N (aˊ,bˊ,cˊ).

[0041] Then according to the air deposition energy E at position N (aˊ, bˊ, cˊ) a (S, E, F) Calculate the air absorbed dose D of a single particle ca (S,E,F): D ca (S,E,F)=E a (S,E,F)×1.602×10 -10 / M a (Gy) .

[0042] in, M a For the quality of air.

[0043] S103, obtaining a conversion coefficient between the absorbed dose of the cell layer and the absorbed dose of the air according to the absorbed dose of the cell layer in the cell culture dish and the absorbed dose of the air.

[0044] The conversion coefficient R(S,E,F) between the absorbed dose in the cell layer and the absorbed dose in the air is determined by the following formula: R(S,E,F) = D c (S,E,F) / D ca (S,E,F); Wherein, R(S,E,F) means that under the conditions of particle type S, particle energy E, and irradiation distance F, the ratio of the absorbed dose of the cell layer at the same position to the absorbed dose of the air is R.

[0045] Specifically, for particle types, due to different particle types, the conversion coefficients of cell layer absorbed dose and air absorbed dose are different; for particle energy E, due to different particle energies, the conversion coefficients of cell layer absorbed dose and air absorbed dose are different; for irradiation distance, since the irradiation distance affects the size of the conversion coefficient of cell layer absorbed dose and air absorbed dose, it affects the accuracy of in vitro cell absorbed dose measurement. The problem existing in the prior art that energy response is a key factor affecting the measurement accuracy of absorbed dose of cell culture fluid directly measured by ionization chamber, and the dose calibration procedure is complicated, making it difficult to obtain an accurate dose calibration factor is solved.

[0046] Example 2

[0047] When the irradiated particle type is photon or electron, the type of the ionization chamber is a graphite cavity ionization chamber; when the filling gas of the graphite cavity ionization chamber is air, the ionization chamber measures the absorbed dose D in the cavity gas at the irradiation position of the cell layer. mg Equal to air absorbed dose D a .

[0048] During measurement, the center of the sensitive volume of the measuring ionization chamber should coincide with the geometric center of the cell layer in the cell culture dish.

[0049] The absorbed dose of the cell layer is obtained by the following formula: D cell =D a× R(S,E,F)×A ; Among them, D cell D is the dose absorbed by the cell layer; a is the air absorption dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the cell layer absorption dose and the air absorption dose obtained in Example 1; A is the emission intensity of the particle source P(S,E,A).

[0050] Example 3

[0051] When the irradiated particle type is photon or electron, the type of the ionization chamber is a graphite cavity ionization chamber. During measurement, the center of the sensitive volume of the measuring ionization chamber should coincide with the geometric center of the cell layer in the cell culture dish.

[0052] When the filling gas of the graphite cavity ionization chamber is gas g, the air absorption dose D is determined according to the absorption dose in the cavity gas. a Determined by the following formula, D a =D mg×S w,g Among them, D mg is the absorbed dose in the cavity gas g obtained by ionization chamber measurement; S w,g is the average mass collision stopping power ratio of the cavity ionization chamber wall material graphite w and the gas g in the cavity; S w,g = , (s / ρ) w is the mass collision stopping power of graphite for secondary electrons or electrons for photons; (s / ρ) g It is the mass collision stopping power of the cavity gas for secondary electrons or electrons of photons.

[0053] According to the air absorption dose D a , the absorbed dose of the cell layer is obtained by the following formula: D cell =D a× R(S,E,F)×A ; Among them, D cell D is the dose absorbed by the cell layer; a is the air absorbed dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorbed dose and the air absorbed dose; A is the emission intensity of the particle source P(S,E,A).

[0054] Example 4

[0055] When the irradiation particle type is neutron, the type of ionization chamber is tissue equivalent ionization chamber. During measurement, the center of the sensitive volume of the measuring ionization chamber should coincide with the geometric center of the cell layer in the cell culture dish.

[0056] The air absorbed dose D is determined from the absorbed dose in the cavity gas by the following formula a , D a =D mg ×(K a / K g ) Among them, D mg K is the absorbed dose in the cavity gas g obtained by measuring the tissue equivalent ionization chamber; a and K g They are the kerma of neutrons in air and in the gas g in the ionization chamber respectively.

[0057] According to the air absorption dose D a , the absorbed dose of the cell layer is obtained by the following formula: D cell =D a× R(S,E,F)×A ; Among them, D cell D is the dose absorbed by the cell layer; a is the air absorbed dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorbed dose and the air absorbed dose; A is the emission intensity of the particle source P(S,E,A).

[0058] Example 5

[0059] When the irradiated particles contain both neutrons and photons; the absorbed dose of neutrons in the cavity gas is obtained using a tissue equivalent ionization chamber; the absorbed dose of photons in the cavity gas is obtained using a hydrogen-free ionization chamber. During measurement, the center of the sensitive volume of the measuring ionization chamber should coincide with the geometric center position of the cell layer in the cell culture dish. The hydrogen-free ionization chamber is a graphite-carbon dioxide ionization chamber or a graphite-argon ionization chamber.

[0060] 1) Determine the neutron air absorption dose D according to the neutron absorption dose in the cavity gas g1 by the following formula: g1,N : D g1,N =

[0061] in, It is the sum of the absorbed doses of neutrons and photons in the gas g1 in the tissue equivalent ionization chamber measured; It is the sum of the absorbed doses of neutrons and photons measured in the gas g2 in the “hydrogen-free” ionization chamber; It is the ratio of the sensitivity of the "hydrogen-free" ionization chamber to neutrons to the gamma-ray sensitivity used for calibration.

[0062] According to the neutron air absorption dose D g1,N Determining the air absorbed dose D a,N D a,N = D g1,N ×(K a / K g1 ) ; Among them, K a and K g1 They are the kerma of neutrons in air and in gas g1.

[0063] According to air absorption dose D a,N , the cell layer absorbed dose of neutrons is obtained by the following formula: D cell =D a，N× R(S,E,F)×A ; Among them, D cell Dose absorption for the cell layer; D a,N is the air absorbed dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorbed dose and the air absorbed dose; A is the emission intensity of the particle source P(S,E,A).

[0064] 2) Determine the air absorption dose D of the photon according to the absorption dose of the photon in the cavity gas g2 by the following formula: g2,p : D g2,p =

[0065] in, It is the sum of the absorbed doses of neutrons and photons in the gas g1 in the tissue equivalent ionization chamber measured; It is the sum of the absorbed doses of neutrons and photons measured in the gas g2 in the “hydrogen-free” ionization chamber; It is the ratio of the sensitivity of the "hydrogen-free" ionization chamber to neutrons to the gamma-ray sensitivity used for calibration.

[0066] D a,P = D g2,p ×S w,g2 Among them, S w,g2 is the average mass collision stopping power ratio of graphite w, the wall material of the “hydrogen-free” ionization chamber, to the gas g2 in the chamber, i.e. S w,g2 = , where (s / ρ)w is the mass collision stopping power of graphite for the secondary electrons of photons; (s / ρ) g2 It is the secondary electron mass collision stopping power of the cavity gas g2 for photons.

[0067] The photon absorbed dose of the cell layer is obtained by the following formula: D cell =D a，P× R(S,E,F)×A ; Among them, D cellDose absorption for the cell layer; D a，N Neutron air absorbed dose values ​​obtained for ionization chamber measurements; D a，P is the photon air absorption dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorption dose and the air absorption dose; A is the emission intensity of the particle source P(S,E,A).

[0068] The invention solves the problem in the prior art that, for neutron external irradiation, multiple correction coefficients need to be applied when directly measuring the absorbed dose of cell culture fluid using an ionization chamber, thereby causing a greater difference between the absorbed dose of the cell culture fluid and the actual cell dose value.

[0069] In the specific implementation process, the present invention integrates various functions into one hardware, and sets up a human-computer interaction system, which only requires manual input to obtain the type of irradiated particles and the irradiation distance, and selects the type of ionization chamber and the type of filling gas of the ionization chamber according to the type of irradiated particles, and inputs the thickness of the cell culture fluid and the cell layer. The data processing module of the hardware automatically calculates the conversion coefficient of the cell layer absorbed dose and the air absorbed dose, as well as the cell layer absorbed dose. During the entire test process, the test system can not only display the test results on the human-computer interaction module, but also export the results to a file, and some test items can be analyzed and counted, that is, automatic comparison, automatic output of results and automatic statistical analysis are realized. It not only solves the problem of large amount of data and long time-consuming detection results of external irradiation in vitro cell absorbed dose detection in the prior art; it further weakens the professional requirements of the operator for external irradiation in vitro cell absorbed dose detection, and greatly improves the accuracy and efficiency of external irradiation in vitro cell absorbed dose detection.

[0070] like Figure 3 As shown, the present invention provides an external irradiation in vitro cell absorbed dose detection system 300, which can be installed in an electronic device. According to the functions to be implemented, the external irradiation in vitro cell absorbed dose detection system 300 can include an irradiation data acquisition unit 310, a measurement unit 320 and an absorbed dose acquisition unit 330. The module described in the present invention can also be referred to as a unit, which refers to a series of computer program segments that can be executed by an electronic device processor and can complete fixed functions, which are stored in the memory of the electronic device.

[0071] In this embodiment, the functions of each module / unit are as follows: An irradiation data acquisition unit 310 is used to acquire the irradiation particle type and the irradiation distance, and select the type of ionization chamber and the type of filling gas of the ionization chamber according to the irradiation particle type; The measuring unit 320 is used to measure the absorbed dose in the cavity gas at the irradiation position of the cell layer by using the ionization chamber according to the type of irradiated particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber; and determine the absorbed dose in the air according to the absorbed dose in the cavity gas; The absorbed dose acquisition unit 330 is used to obtain the cell layer absorbed dose based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose; the conversion coefficient between the cell layer absorbed dose and the air absorbed dose is obtained through a preset in vitro cell geometric model using a particle transport simulation program.

[0072] The external irradiation in vitro cell absorbed dose detection system 300 of the present invention comprehensively considers the influence of particle type, energy level, and thickness of cell culture fluid and cell layer on cell absorbed dose by using a particle transport simulation program; first, the conversion coefficient between in vitro cell absorbed dose and air absorbed dose under different irradiation conditions is determined. Then, the air absorbed dose is directly measured using an ionization chamber, and the measurement result of the air absorbed dose is converted into the absorbed dose of the in vitro cell by using the conversion coefficient between the cell layer absorbed dose and the air absorbed dose obtained previously. Compared with the traditional method of directly using an ionization chamber to measure the absorbed dose of the cell culture fluid, the present invention not only simplifies the measurement process, but also significantly improves the accuracy of the in vitro cell absorbed dose measurement.

[0073] like Figure 4 As shown, the present invention provides an electronic device 4 capable of implementing a method for detecting external irradiation in vitro cell absorbed dose.

[0074] The electronic device 4 may include a processor 40 , a memory 41 and a bus, and may also include a computer program stored in the memory 41 and executable on the processor 40 , such as an external irradiation in vitro cell absorbed dose detection program 42 .

[0075] The memory 41 includes at least one type of readable storage medium, including flash memory, mobile hard disk, multimedia card, card-type memory (such as SD or DX memory, etc.), magnetic memory, disk, optical disk, etc. In some embodiments, the memory 41 may be an internal storage unit of the electronic device 3, such as a mobile hard disk of the electronic device 4. In other embodiments, the memory 31 may also be an external storage device of the electronic device 4, such as a plug-in mobile hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (SecureDigital, SD) card, a flash card (Flash Card), etc. equipped on the electronic device 3. Further, the memory 41 may also include both an internal storage unit and an external storage device of the electronic device 4. The memory 41 may not only be used to store application software and various types of data installed in the electronic device 4, such as the code of the external irradiation in vitro cell absorption dose detection program, but also be used to temporarily store data that has been output or is to be output.

[0076] The processor 40 may be composed of an integrated circuit in some embodiments, for example, a single packaged integrated circuit, or a plurality of packaged integrated circuits with the same or different functions, including one or more central processing units (CPUs), microprocessors, digital processing chips, graphics processors, and combinations of various control chips, etc. The processor 40 is the control core (Control Unit) of the electronic device, and uses various interfaces and lines to connect various components of the entire electronic device, and executes various functions and processes data of the electronic device 4 by running or executing programs or modules (such as external irradiation in vitro cell absorption dose detection programs, etc.) stored in the memory 41, and calling data stored in the memory 41.

[0077] The bus may be a peripheral component interconnect (PCI) bus or an extended industry standard architecture (EISA) bus, etc. The bus may be divided into an address bus, a data bus, a control bus, etc. The bus is configured to realize connection and communication between the memory 41 and at least one processor 40, etc.

[0078] Figure 4 Only an electronic device with components is shown, and those skilled in the art will understand that Figure 4The structure shown does not constitute a limitation on the electronic device 4, and may include fewer or more components than shown in the figure, or combine certain components, or arrange the components differently.

[0079] For example, although not shown, the electronic device 4 may also include a power source (such as a battery) for supplying power to various components. Preferably, the power source may be logically connected to the at least one processor 40 through a power management device, so that the power management device can realize functions such as charging management, discharging management, and power consumption management. The power source may also include any components such as one or more DC or AC power sources, recharging devices, power failure detection circuits, power converters or inverters, and power status indicators. The electronic device 4 may also include a variety of sensors, Bluetooth modules, Wi-Fi modules, etc., which will not be described in detail here.

[0080] Furthermore, the electronic device 4 may also include a network interface. Optionally, the network interface may include a wired interface and / or a wireless interface (such as a WI-FI interface, a Bluetooth interface, etc.), which is generally used to establish a communication connection between the electronic device 3 and other electronic devices.

[0081] Optionally, the electronic device 4 may further include a user interface, which may be a display, an input unit (such as a keyboard), or a standard wired interface or a wireless interface. Optionally, in some embodiments, the display may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, and an OLED (Organic Light-Emitting Diode) touch device. The display may also be appropriately referred to as a display screen or a display unit, which is used to display information processed in the electronic device 4 and to display a visual user interface.

[0082] It should be understood that the embodiment is for illustration only and the scope of the patent application is not limited to this structure.

[0083] The external irradiation in vitro cell absorption dose detection program 42 stored in the memory 41 in the electronic device 4 is a combination of multiple instructions. When running in the processor 40, it can achieve: obtaining the irradiation particle type and irradiation distance, and selecting the type of ionization chamber and the type of filling gas of the ionization chamber according to the irradiation particle type; using the ionization chamber to measure the absorption dose in the cavity gas at the cell layer irradiation position according to the irradiation particle type, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber; determining the air absorption dose according to the absorption dose in the cavity gas; obtaining the cell layer absorption dose based on the air absorption dose and the pre-acquired conversion coefficient between the cell layer absorption dose and the air absorption dose; wherein the conversion coefficient between the cell layer absorption dose and the air absorption dose is obtained by a preset in vitro cell geometric model using a particle transport simulation program.

[0084] Specifically, the specific implementation method of the processor 40 for the above instructions can refer to Figure 1 The description of the relevant steps in the corresponding embodiment is not repeated here. It should be emphasized that in order to further ensure the privacy and security of the above-mentioned external irradiation in vitro cell absorption dose detection procedure, the above-mentioned database can process data and store it in the node of the blockchain where the server cluster is located.

[0085] Furthermore, if the module / unit integrated in the electronic device 4 is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. The computer-readable medium may include: any entity or device capable of carrying the computer program code, a recording medium, a USB flash drive, a mobile hard disk, a magnetic disk, an optical disk, a computer memory, and a read-only memory (ROM).

[0086] The embodiment of the present invention also provides a computer-readable storage medium, which can be non-volatile or volatile, and stores a computer program. When the computer program is executed by a processor, it is implemented as follows: obtaining the type of irradiated particles and the irradiation distance, and selecting the type of ionization chamber and the type of filling gas of the ionization chamber according to the type of irradiated particles; using the ionization chamber to measure the absorbed dose in the cavity gas at the cell layer irradiation position according to the type of irradiated particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber; determining the air absorbed dose according to the absorbed dose in the cavity gas; obtaining the cell layer absorbed dose based on the air absorbed dose and the conversion coefficient of the pre-acquired cell layer absorbed dose and air absorbed dose; wherein the conversion coefficient of the cell layer absorbed dose and the air absorbed dose is obtained by using a preset in vitro cell geometric model based on a particle transport simulation program. Specifically, the specific implementation method when the computer program is executed by the processor can refer to the description of the relevant steps in the external irradiation in vitro cell absorbed dose detection method in the embodiment, which will not be repeated here.

[0087] In the several embodiments provided by the present invention, it should be understood that the disclosed devices, apparatuses and methods can be implemented in other ways. For example, the device embodiments described above are only schematic, for example, the division of the modules is only a logical function division, and there may be other division methods in actual implementation.

[0088] The modules described as separate components may or may not be physically separated, and the components shown as modules may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0089] In addition, each functional module in each embodiment of the present invention may be integrated into one processing unit, each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of hardware plus software functional modules.

[0090] It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention.

[0091] Therefore, no matter from which point of view, the embodiments should be regarded as illustrative and non-restrictive, and the scope of the present invention is limited by the appended claims rather than the above description, so it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any attached figure mark in the claims should not be regarded as limiting the claims involved.

[0092] The blockchain referred to in this invention is a new application mode of computer technologies such as distributed data storage, peer-to-peer transmission, consensus mechanism, encryption algorithm, etc. Blockchain is essentially a decentralized database, a string of data blocks generated by cryptographic methods. Each data block contains a batch of network transaction information, which is used to verify the validity of its information (anti-counterfeiting) and generate the next block. Blockchain can include the underlying blockchain platform, platform product service layer, and application service layer.

[0093] In addition, it is clear that the word "comprising" does not exclude other units or steps, and the singular does not exclude the plural. Multiple units or devices stated in a device claim can also be implemented by one unit or device through software or hardware. The second and other words are used to indicate names, but not to indicate any particular order.

[0094] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for detecting external irradiation in vitro cell absorbed dose, characterized in that the method include: Acquire the irradiated particle type and irradiation distance, and select the type of ionization chamber and the type of filling gas of the ionization chamber according to the irradiated particle type; According to the type of irradiated particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, using the ionization chamber to measure the absorbed dose in the cavity gas at the irradiation position of the cell layer; and determining the absorbed dose in the air according to the absorbed dose in the cavity gas; The cell layer absorbed dose is obtained based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose; wherein the conversion coefficient between the cell layer absorbed dose and the air absorbed dose is obtained by using a preset in vitro cell geometric model based on a particle transport simulation program.

2. The method for detecting external irradiation in vitro cell absorbed dose according to claim 1, characterized in that: The method of obtaining the conversion coefficient between the cell layer absorbed dose and the air absorbed dose by using a particle transport simulation program through a preset in vitro cell geometric model includes: Establishing an in vitro cell geometric model; the in vitro cell geometric model includes a cell culture dish, a cell layer, and a cell culture fluid layer; Using a particle transport simulation program, the absorbed dose of the cell layer in the cell culture dish is obtained according to the particle type, particle energy and irradiation distance through an in vitro cell geometric model; the absorbed dose of air is obtained according to the particle type, particle energy and irradiation distance using a particle transport simulation program; According to the absorbed dose of the cell layer in the cell culture dish and the absorbed dose of the air, a conversion coefficient between the absorbed dose of the cell layer and the absorbed dose of the air is obtained.

3. The method for detecting external irradiation in vitro cell absorbed dose according to claim 1, characterized in that: In the process of measuring the absorbed dose in the cavity gas at the irradiation position of the cell layer using the ionization chamber according to the type of irradiation particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, When the irradiated particles are photons or electrons, the ionization chamber is a graphite cavity ionization chamber; When the filling gas of the graphite cavity ionization chamber is air, the absorbed dose in the cavity gas at the irradiation position of the cell layer measured by the ionization chamber is equal to the absorbed dose in the air; When the filling gas of the graphite cavity ionization chamber is gas g, the air absorption dose D is determined according to the absorption dose in the cavity gas. a Determined by the following formula, D a =D mg× S w,g Among them, D mg is the absorbed dose in the cavity gas g obtained by ionization chamber measurement; S w,g is the average mass collision stopping power ratio of the cavity ionization chamber wall material graphite w and the gas g in the cavity; S w,g = , (s / ρ) w is the mass collision stopping power of graphite for secondary electrons or electrons for photons; (s / ρ) g It is the mass collision stopping power of the cavity gas for secondary electrons or electrons of photons.

4. The method for detecting external irradiation in vitro cell absorbed dose according to claim 1, characterized in that: In the process of measuring the absorbed dose in the cavity gas at the irradiation position of the cell layer using the ionization chamber according to the type of irradiation particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, When the irradiation particle type is neutron, the type of ionization chamber is tissue equivalent ionization chamber; Determine the air absorbed dose D based on the absorbed dose in the cavity gas a Determined by the following formula, D a =D mg ×(K a / K g ) Among them, D mg K is the absorbed dose in the cavity gas g obtained by measuring the tissue equivalent ionization chamber; a and K g They are the kerma of neutrons in air and in the gas g in the ionization chamber respectively.

5. The method for detecting external irradiation in vitro cell absorbed dose according to claim 4, characterized in that: In the process of measuring the absorbed dose in the cavity gas at the irradiation position of the cell layer using the ionization chamber according to the type of irradiation particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber, When the irradiating particles contain both neutrons and photons; The absorbed dose of neutrons in the cavity gas is obtained by using a tissue equivalent ionization chamber; the absorbed dose of photons in the cavity gas is obtained by using a hydrogen-free ionization chamber; The air absorption dose of neutrons is determined according to the absorption dose of neutrons in the cavity gas, and the air absorption dose of photons is determined according to the absorption dose of photons in the cavity gas.

6. The method for detecting external irradiation in vitro cell absorbed dose according to claim 5, characterized in that: The hydrogen-free ionization chamber is a graphite-carbon dioxide ionization chamber or a graphite-argon ionization chamber.

7. The method for detecting external irradiation in vitro cell absorbed dose according to claim 1, characterized in that: In the process of obtaining the cell layer absorbed dose based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose, When the particle source is neutrons, photons or electrons, the absorbed dose of the cell layer is obtained by the following formula: D cell =D a× R(S,E,F)×A ; Among them, D cell D is the dose absorbed by the cell layer; a is the air absorption dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorption dose and the air absorption dose; A is the emission intensity of the particle source P(S,E,A); When the particle source contains both neutrons and photons, the neutron absorption dose in the cell layer is obtained by the following formula: D cell =D a，N× R(S,E,F)×A ; The photon absorbed dose of the cell layer is obtained by the following formula: D cell =D a，P× R(S,E,F)×A ; Among them, D cell Dose absorption for the cell layer; D a，N Neutron air absorbed dose values ​​obtained for ionization chamber measurements; D a，P is the photon air absorption dose value obtained by ionization chamber measurement; R(S,E,F) is the conversion coefficient between the corresponding cell layer absorption dose and the air absorption dose; A is the emission intensity of the particle source P(S,E,A).

8. The method for detecting external irradiation in vitro cell absorbed dose according to claim 2, characterized in that: The cell layer in the ex vivo cell geometry model is composed of tissue equivalent materials.

9. A system for detecting external irradiation in vitro cell absorbed dose, characterized in that: For implementing the method for detecting external irradiation in vitro cell absorbed dose as claimed in any one of claims 1 to 8, the system comprises: an irradiation data acquisition unit, used to acquire the irradiation particle type and the irradiation distance, and select the type of the ionization chamber and the type of filling gas of the ionization chamber according to the irradiation particle type; A measuring unit, configured to measure the absorbed dose in the cavity gas at the irradiation position of the cell layer by using the ionization chamber according to the type of irradiated particles, the type of ionization chamber, the irradiation distance and the type of filling gas of the ionization chamber; and determine the absorbed dose in the air according to the absorbed dose in the cavity gas; The absorbed dose acquisition unit is used to obtain the cell layer absorbed dose based on the air absorbed dose and the pre-acquired conversion coefficient between the cell layer absorbed dose and the air absorbed dose; wherein the conversion coefficient between the cell layer absorbed dose and the air absorbed dose is obtained by using a preset in vitro cell geometric model based on a particle transport simulation program.

10. An electronic device, characterized in that: The electronic device comprises: at least one processor; and, a memory communicatively connected to the at least one processor; wherein, The memory stores instructions executable by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can perform the steps in the method for detecting external irradiation in vitro cell absorbed dose as described in any one of claims 1 to 8.

Images