Photometric point calibration method and system of full-automatic biochemical analyzer based on cuvette delay strategy

Abstract

The invention discloses a method and a system for calibrating a light measuring point of a full-automatic biochemical analyzer based on a cuvette delay strategy. The method comprises the following steps: configuring a cuvette to be detected; setting a plurality of different delay times for the same cuvette based on the cuvette delay strategy; obtaining the distance from the light measuring point of the current cuvette to the optical detection position during detection, and selecting optimized delay time according to a distance comparison result to carry out delay detection so as to obtain a stable AD value; in the testing process, each cuvette passes through an optical detection position, absorbance data currently read by each cuvette is used as a photometric point, a time point for reading the data of the photometric point after a current cuvette serial number optocoupler is triggered is used as a delay point, the jumping photometric point is identified, and different delay times are selected; the problem that the photometric point of the full-automatic biochemical analyzer jumps is solved by delay adjustment of the cuvette, so that the originally unstable photometric point can read stable data, and the detection result is more accurate and real.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical automatic detection equipment, and in particular to a photometric point calibration method and system of a full-automatic biochemical analyzer based on a cuvette delay strategy. Background Art

[0002] In the fully automatic biochemical analyzer, time delay detection is an important part. Whether the delay point setting is reasonable directly affects the accuracy and precision of the test results. For a colorimetric cup, its time delay detection curve generally presents Figure 1 As shown in the curve trend, we need to set the delay point reasonably so that the delay point falls on the platform area of ​​the delay curve. Only then can the AD value read out of this platform area be stable and accurate.

[0003] In order to obtain the appropriate delay point, in general, when performing a delay test, the colorimetric cup that needs to obtain the delay will stop at a position far away from the optical position. In this way, the delay curve obtained will be roughly like this: Figure 1 As shown, it is relatively easy to find a suitable delay point. In general, the same colorimetric cup of the fully automatic biochemical analyzer has only one delay point, which is obtained by the method introduced above. This delay point is applicable to most photometric points, but not to photometric points that are prone to jumping. For photometric points that are prone to jumping, the AD value read using this delay is inaccurate.

[0004] like Figure 2 As shown in the figure, a normal light metering point can read a stable AD value by using the delay point 113. For a jumping light metering point, the delay curve is as follows Figure 3 As shown in the figure, it is necessary to use the 135 delay point to just land on the delay curve platform. If the AD value is read at the 113 delay point, the AD value read is on the rising edge, which is not a stable and accurate value, which may lead to inaccurate test results. For those metering points that are prone to jumping, it is found through analysis that the data read this time will be inaccurate.

[0005] At present, there are two popular solutions to this problem in the industry: one is to correct the AD value read by the jumping light metering point through software, that is, according to the change pattern of the values ​​before and after the jumping light metering point, the data of the light metering point is inferred and corrected. The disadvantage is that the data read out at the jumping light metering point is calculated by the software, which cannot fully reflect the real situation. The value obtained is based on the changing trend, which may not be completely in line with this trend in the actual process. If this jumping light metering point happens to be the point required for the result calculation, it will affect the accuracy of the result; the other is to adjust the optical detection position and the overall layout to avoid the occurrence of jumping light metering points as much as possible. The disadvantage is that the adjustment of the optical detection position may completely avoid all jumping light metering points, but this method has extremely high requirements on the layout and brings great difficulties to the structural design.

[0006] In summary, the present invention is necessary to propose a photometric point calibration method for a fully automatic biochemical analyzer based on a cuvette delay strategy, and solve the problem of photometric point jitter of the fully automatic biochemical analyzer by adjusting the cuvette delay. Summary of the invention

[0007] The purpose of the present invention is to provide a photometric point calibration method and system for a fully automatic biochemical analyzer based on a colorimetric cup delay strategy, which solves the problem of photometric point jitter of the fully automatic biochemical analyzer by adjusting the colorimetric cup delay, so that the originally unstable photometric point can read stable data, making the detection result more accurate and true.

[0008] The present invention provides a photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy, wherein the fully automatic biochemical analyzer comprises a reaction disk having an inner ring and an outer ring, wherein the movement track of the reaction disk comprises optical detection positions corresponding to the inner ring and the outer ring, wherein the optical detection positions corresponding to the inner ring and the outer ring are spaced apart by N cuvette positions in a clockwise or counterclockwise direction, and the photometric point calibration steps of the fully automatic biochemical analyzer are as follows: Configure the cuvette to be tested; Based on the cuvette delay strategy, multiple different delay times are set for the same cuvette; Obtain the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and select the optimized delay time for delay detection according to the comparison distance result to obtain a stable AD value; During the test, each time the colorimetric disk rotates one circle, each colorimetric cup passes through the optical detection position, and the absorbance data currently read by each colorimetric cup is used as the photometric point. The time point when the photometric point data is read after the optical coupler of the current colorimetric cup sequence is triggered is used as the delay point, and the jumping photometric point is identified and different delay times are selected; Among them, when the colorimetric disk needs to continuously switch between the two states of rotation and stop during the test, acceleration and deceleration operations are performed each time the rotation is started and stopped. During the acceleration and deceleration operation, if there is a sample being tested in the colorimetric cup at the photoelectric detection position, the currently detected photometric point data is the jumping photometric point data.

[0009] Preferably, the method of setting a plurality of different delay times for the same cuvette based on the cuvette delay strategy further comprises: Configure the first target distance from the optical detection position to the first reading delay of the first sequence number cuvette; Configure the second target distance from the optical detection position for the second reading delay of the second numbered cuvette; Configure the Nth target distance from the optical detection position to the Nth reading delay of the Nth numbered cuvette; Among them, the first target distance, the second target distance and the Nth target distance are all target distances between the colorimetric cup and the optical detection position when the reaction disk is in a stopped state, and the target distances enable the reaction disk to have different distances in the acceleration stage, deceleration stage and uniform speed stage to match different delays.

[0010] Preferably, the method of obtaining the distance between the photometric point of the current cuvette and the optical detection position during detection, and selecting an optimized delay time for delay detection according to the distance comparison result to obtain a stable AD value includes: If the delay of all cuvettes being away from the optical detection target distance is detected, the cuvette with the current serial number is placed at a position away from the optical detection position target distance and then rotated; During the rotation process, all AD data of the colorimetric cup with the current serial number are read to form a delay curve; Acquire a stable target point as a delay point based on the delay curve; The next numbered cuvette is placed at a position at a target distance from the optical detection position and the above process is continued until all cuvettes are traversed to complete a set of delayed detection; According to the above operation process, the motion parameters of the instrument are obtained to calculate the distance of the colorimetric cup from the optical detection position in the acceleration stage and the deceleration stage, so as to identify the jumping photometric point and select the corresponding delay time; The target distance includes the relative distance between the colorimetric cup and the optical detection position when the reaction disk is in the acceleration stage and the reaction disk is in the uniform speed operation stage.

[0011] Preferably, the step of obtaining the motion parameters of the instrument according to the above operation process to calculate the distance between the cuvette and the optical detection position in the acceleration stage and the deceleration stage includes: Obtain the operating parameters of the fully automatic biochemical analyzer configuration; Calculate the colorimetric cup distance optical detection position corresponding to the acceleration stage and convert it into the corresponding colorimetric cup position number C=((V1+V2)*T1*α*m) / 720*n*i; wherein the operating parameters include initial velocity V1, uniform velocity V2, motor subdivision n, motor step distance α, reaction disk rotation transmission ratio i, reaction disk colorimetric cup number m, and 720 is a constant for angle calculation.

[0012] Preferably, under normal circumstances, the first delay time is configured as the uniform speed stage. If the identified light metering point is in the acceleration stage, the second delay time will be configured as the acceleration stage. According to the instrument layout, the jumping light metering point is directly identified, and a different third delay time is selected and configured.

[0013] Preferably, in the double-circle system, if the distance between the 6th stage cleaning position of the outer circle W6 and the optical detection position is only the first distance, then during each cuvette blank test, the current cuvette position is in the acceleration stage of the reaction disk rotation; If all the photometric points share a delay point and unstable AD values ​​are read during the rising edge, two groups of delay operations are set for each colorimetric cup in the outer circle; The delay operation is to detect a set of delay values ​​when each colorimetric cup is at a first distance from the optical detection position, and to detect a set of delay values ​​when each colorimetric cup is at a second distance from the optical detection position, and both sets of delay values ​​are stored in the system. During the test, the outer ring W6 is identified as a beating photometric point and different delay values ​​are selected to obtain a stable AD value; Among them, the first distance is in the acceleration stage of the reaction disk, and the first distance is converted to the first target number of cups according to the acceleration stroke divided by the distance between each cuvette cup position, and the second distance is in the uniform speed operation stage of the reaction disk, and the second distance is converted to the second target number of cups according to the uniform speed operation stroke divided by the distance between each cuvette cup position; W6 the 6th stage cleaning position represents the blank position of each cuvette test cup during the sample test of the fully automatic biochemical analyzer; the number of cups corresponding to the acceleration and deceleration strokes refers to the number of cuvettes passed by the reaction disk during the process of accelerating from the initial speed to the uniform speed or decelerating from the uniform speed to stopping.

[0014] Preferably, the absorbance calculation formula is as follows: absorbance of the current cuvette = -2*log (AD value of the current photometric point / AD value of the cup blank), wherein W1-W9 means that each cuvette is subjected to 9-step cleaning after the test is completed to ensure that the cuvette is clean and put into use, and W6, the 6th step cleaning, is after cleaning and water injection. During the next rotation process, the optical data of the cuvette will be read, and the AD value of the optical data is used to determine whether the current cuvette is clean and can be put into use. When it is determined that it can be put into use, reagents and samples are added in the subsequent process for reaction, and the AD value of the optical data after the 6th step cleaning read in the process is the cup blank.

[0015] The present invention provides a photometric point calibration system of a fully automatic biochemical analyzer based on a cuvette delay strategy, wherein the fully automatic biochemical analyzer comprises a reaction disk having an inner ring and an outer ring, wherein the movement track of the reaction disk comprises optical detection positions corresponding to the inner ring and the outer ring, wherein the optical detection positions corresponding to the inner ring and the outer ring are spaced apart by N cuvette positions in a clockwise or counterclockwise direction, and the photometric point calibration system of the fully automatic biochemical analyzer comprises: A configuration module, used for configuring the colorimetric cup to be detected; A delay module is used to set multiple different delay times for the same colorimetric cup based on the colorimetric cup delay strategy; The detection module is used to obtain the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and select the optimized delay time to perform delay detection according to the comparison distance result to obtain a stable AD value; During the test, each time the colorimetric disk rotates one circle, each colorimetric cup passes through the optical detection position, and the absorbance data currently read by each colorimetric cup is used as the photometric point. The time point when the photometric point data is read after the optical coupler of the current colorimetric cup sequence is triggered is used as the delay point, and the jumping photometric point is identified and different delay times are selected; Among them, when the colorimetric disk needs to continuously switch between the two states of rotation and stop during the test, acceleration and deceleration operations are performed each time the rotation is started and stopped. During the acceleration and deceleration operation, if there is a sample being tested in the colorimetric cup at the photoelectric detection position, the currently detected photometric point data is the jumping photometric point data.

[0016] The present invention provides an electronic device, comprising: A memory, the memory being used to store a processing program; A processor, wherein when executing the processing program, the processor implements the photometric point calibration method of the full-automatic biochemical analyzer based on the cuvette delay strategy as described in the embodiment of the present invention.

[0017] The present invention provides a readable storage medium, characterized in that a processing program is stored on the readable storage medium, and when the processing program is executed by a processor, a photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as described in an embodiment of the present invention is implemented.

[0018] With respect to the prior art, the present invention has the following beneficial effects: The invention proposes a photometric point calibration method for a full-automatic biochemical analyzer based on a cuvette delay strategy. The cuvette delay strategy can effectively solve the problem of photometric point jitter in the full-automatic biochemical analyzer, so that the originally unstable photometric point can read stable data, which is helpful to improve the accuracy and authenticity of the detection result, thereby providing a more reliable basis for medical diagnosis; by configuring a plurality of different delay times, and selecting the optimized delay time according to the comparison distance result to perform delay detection, so as to obtain a stable AD value, the optimized test process can improve the test efficiency, reduce unnecessary repeated tests, and save time and resources; it is suitable for a full-automatic biochemical analyzer with an inner ring and an outer ring of reaction disks, and this method can be applied to perform photometric point calibration regardless of the motion trajectory in the clockwise or counterclockwise direction, so that the scheme has strong adaptability and can be widely used in different types of full-automatic biochemical analyzers; by solving the photometric point jitter problem, the overall performance of the full-automatic biochemical analyzer can be improved, and the stable photometric point data can help improve the measurement accuracy and stability of the instrument, thereby providing users with more accurate and reliable detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The delay detection curve in the embodiment of the present invention generally presents a curve trend diagram; Figure 2-3 A curve trend diagram of a normal light measurement point using a delay point in an embodiment of the present invention; Figure 4 This is a schematic diagram of the layout of the reaction disk in an embodiment of the present invention; Figure 5 The figure is a flow chart of a photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy in an embodiment of the present invention. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] In order to simplify the drawings, only the parts related to the present invention are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically drawn or marked.

[0022] In the present invention, the directions or positional relationships indicated by terms such as "upper", "lower", "left", "right", "inside", "outside", and "top" are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or component referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limitations on the present invention.

[0023] In the present invention, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. It can be directly connected or indirectly connected through an intermediate medium.

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the specific implementation of the present invention will be described below with reference to the accompanying drawings.

[0025] The embodiment of the present invention provides a method for calibrating the photometric point of a fully automatic biochemical analyzer based on a cuvette delay strategy, such as Figure 4 As shown, the fully automatic biochemical analyzer includes a reaction disk with an inner ring and an outer ring, and the movement trajectory of the reaction disk has optical detection positions corresponding to the inner ring and the outer ring, and the optical detection positions corresponding to the inner ring and the outer ring are spaced N colorimetric cup positions in a clockwise or counterclockwise direction. The photometric point calibration steps of the fully automatic biochemical analyzer are as follows: S1: Configure the cuvette to be tested; S2: Setting multiple different delay times for the same cuvette based on the cuvette delay strategy; S3: Obtain the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and select the optimized delay time to perform delay detection according to the comparison distance result to obtain a stable AD value; During the test, each time the colorimetric disk rotates one circle, each colorimetric cup passes through the optical detection position, and the absorbance data currently read by each colorimetric cup is used as the photometric point. The time point when the photometric point data is read after the optical coupler of the current colorimetric cup sequence is triggered is used as the delay point, and the jumping photometric point is identified and different delay times are selected; Among them, when the colorimetric disk needs to continuously switch between the two states of rotation and stop during the test, acceleration and deceleration operations are performed each time the rotation is started and stopped. During the acceleration and deceleration operation, if there is a sample being tested in the colorimetric cup at the photoelectric detection position, the currently detected photometric point data is the jumping photometric point data.

[0026] The colorimetric cup delay strategy adopted in this embodiment: under normal circumstances, it is configured as the first delay time of the uniform speed stage. If the identified light measuring point is in the acceleration stage, it will be configured as the second delay time of the acceleration stage; according to the instrument layout, the jumping light measuring point is directly identified, and a different third delay time is selected and configured.

[0027] Specifically, the step of obtaining the motion parameters of the instrument according to the above operation process to calculate the distance between the cuvette and the optical detection position in the acceleration stage and the deceleration stage includes: Obtain the operating parameters of the fully automatic biochemical analyzer configuration; Calculate the colorimetric cup distance optical detection position corresponding to the acceleration stage and convert it into the corresponding colorimetric cup position number C=((V1+V2)*T1*α*m) / 720*n*i; wherein the operating parameters include initial velocity V1, uniform velocity V2, motor subdivision n, motor step distance α, reaction disk rotation transmission ratio i, reaction disk colorimetric cup number m, 720 is a constant for angle calculation, and during implementation, set the operating parameters: initial velocity V1 (PPS) is 800, uniform velocity V2 (PPS) is 16000, acceleration time T1 (S) is 0.075, motor subdivision n is 16, motor step distance α is 1.8, reaction disk rotation transmission ratio i is 20.5, reaction disk colorimetric cup number m is 205, and the acceleration stroke Y=(V1+V2)*T1*0 .5=630, the angle of rotation of the motor in the acceleration stage α'=360*Y1 / ((360 / α)*n)=70.875, the angle of rotation of the reaction disk α''=α' / i=3.457317073, the angle of one cup position on the reaction disk a=360 / m=1.756097561, the cup position C in the acceleration stage=α'' / a=1.96875, through the above calculation method, according to the specific instrument parameters and test requirements, follow the steps in the scheme to realize the photometric point calibration of the fully automatic biochemical analyzer with the colorimetric cup delay strategy, adaptively solve the problem of photometric point jump, improve the overall performance of the fully automatic biochemical analyzer, help improve the measurement accuracy and stability of the instrument, and thus provide users with more accurate and reliable test results.

[0028] like Figure 4The incubation plate is shown as a colorimetric plate. There are several colorimetric cups on this plate. All colorimetric cups are placed in a 37℃ environment, and the photometric position is facing one of the colorimetric cups. During the operation of the instrument, the colorimetric plate rotates in a fixed direction, and a fixed number of cup positions are rotated each time. After rotating n times, it will rotate one circle, and a fixed number of cup positions will be advanced or reversed each circle. During the operation, samples and reagents will be added to the colorimetric cups for reaction. During the operation of the instrument, the absorbance data of the tested colorimetric cups will continue to be detected by optical components. Each time the colorimetric plate rotates one circle, each cup will pass through the optical detection position once, and each cup will read an absorbance data, which is called a photometric point. There will be dozens of photometric points in a test process. The time point at which the data at these photometric points are read is the delay point. After triggering the home optical coupler, the instrument knows the origin position, and then determines the current position by triggering the serial optical coupler. The delay point is how long it takes for the colorimetric cup to be tested to read the data after triggering the serial optical coupler. The delay curve is drawn by continuously reading data for a period of time. It can be seen when the serial optocoupler is triggered. After the serial optocoupler is triggered, a stable platform is selected to obtain the value through the algorithm to obtain the delay point. This time point is the absorbance data of this colorimetric cup read at this long interval after each serial optocoupler is triggered.

[0029] Table 1 Fully automatic biochemical analyzer sample test data table Outer ring W1 W2 W3 W4 W5 W6 W7 W8 W9 Inner Circle W1 W2 W3 W4 W5 W6 W7 W8 W9 Cup number 1 206 205 204 203 202 201 200 199 197 196 As shown in Table 1, the W6 position is the blank position of each cuvette test cup during the sample test of the fully automatic biochemical analyzer. The double-circle reaction system is shown here, and the same is true for the single-circle reaction system. In this double-circle system, the outer circle W6 position is only one cup position away from the optical detection position. Therefore, the outer circle reaction system will appear in the acceleration stage of the reaction disk rotation during each cuvette blank test. At this time, if a delay point is shared with other photometric points, Figure 2-3 In this case, unstable AD values ​​will be read on the rising edge, which will lead to inaccurate results and large fluctuations in the result values ​​of the outer ring reaction system. In the inner ring reaction system, the W6 position is 2 cup positions away from the optical detection position. When detecting this photometric point, it has entered the uniform speed operation stage, and the outer ring situation will not occur.

[0030] In order to overcome the occurrence of the above situation, the present embodiment adopts a cuvette delay strategy: configuring a first target distance from the optical detection position for the first read delay of the cuvette with the first serial number; configuring a second target distance from the optical detection position for the second read delay of the cuvette with the second serial number; configuring an Nth target distance from the optical detection position for the Nth read delay of the cuvette with the Nth serial number; wherein the first target distance, the second target distance and the Nth target distance are all target distances between the cuvette and the optical detection position when the reaction disk is in a stopped state, and the target distances enable the reaction disk to have different distances in the acceleration stage, the deceleration stage and the uniform speed stage to match different delays. It can be understood that in order to overcome the above defects, two sets of delays need to be set for each colorimetric cup in the outer ring. When setting the delay, each colorimetric cup needs to detect a set of delay values ​​when it is 1 cup position away from the optical detection position, that is, in the acceleration stage of the reaction disk, and detect a set of delay values ​​when it is 15 cup positions away from the optical detection position, that is, in the uniform speed operation stage of the reaction disk. Both sets of delay values ​​are stored in the system. The above distance description is converted into a cup position description during the actual test. In the actual test process, according to the identified jumping photometric point, as shown in Table 1, it is identified that the outer ring W6 detection is a jumping photometric point, and different delay values ​​are selected to obtain a stable AD value. Among them, the reaction disk is switched between continuous rotation and stop state, and the distance from the optical detection position refers to: after the reaction disk stops, the distance between the colorimetric cup to be detected and the optical position. If this distance is ≤2 cup positions, it is determined to be the acceleration stage. Acceleration and deceleration are calculated by motion parameters to obtain acceleration and deceleration strokes, which are converted into cup positions through this stroke. The currently set motion speed and acceleration and deceleration time are calculated to be 2 cup positions. The distance of the reaction disk's start and stop rotation minus the distance of acceleration and deceleration is the same as the distance of uniform speed. For example, the fully automatic biochemical analyzer starts and stops and rotates to 41 positions. Minus 2 positions for acceleration and deceleration, the remaining 37 positions are uniform speed. For this product, distances between 3 and 38 are all uniform speeds, but 15 cup positions are selected.

[0031] The delayed reading process is as follows Figure 5 As shown: if the delay of all colorimetric cups from the optical detection target distance is detected, the colorimetric cup with the current serial number is placed at a position with a target distance from the optical detection position and then rotated; during the rotation process, all AD data of the colorimetric cup with the current serial number is read to form a delay curve; based on the delay curve, a stable target point is obtained as a delay point; the colorimetric cup with the next serial number is placed at a position with a target distance from the optical detection position and the above process is continued until all colorimetric cups are traversed to complete a set of delay detection; According to the above operation process, the motion parameters of the instrument are obtained to calculate the distance between the colorimetric cup and the optical detection position in the acceleration stage and the deceleration stage, so as to identify the jumping photometric point and select the corresponding delay time; wherein, the target distance includes the relative distance between the colorimetric cup and the optical detection position when the reaction disk is in the acceleration stage and the reaction disk is in the uniform speed operation stage. According to the above process, according to whether the identified jumping photometric point is generated in the acceleration stage of the reaction disk rotation or the deceleration stage of the reaction disk rotation, the problem of unstable AD value reading can be avoided by delay setting. After the layout of the instrument is completed and the operation mode is determined, the jumping photometric point can be identified. The operation rules of the instrument are fixed. According to the motion parameters, the acceleration and deceleration stroke can be calculated. The conversion into cup positions will know how many cup positions are needed for acceleration and deceleration. According to the operation rules, the position from the optical during the operation process will be known, so that which photometric points are jumping can be identified. The specific judgment method of whether it is generated in the acceleration stage or the deceleration stage has been explained above. For example, in this embodiment, the cup positions 1 to 2 away from the optical detection position are generated in the acceleration stage, and the cup positions 40 to 41 away from the optical detection position are generated in the deceleration stage.

[0032] For example, to detect the delay of all colorimetric cups 2 cup positions away from the optical detection, then colorimetric cup No. 1 will stop at the position 2 cup positions away from the optical detection and start rotating. During the rotation process, all AD data of colorimetric cup No. 1 will be read to form a delay curve, and then a stable point on the curve will be set as the delay point. Then colorimetric cup No. 2 will stop at the position 2 cup positions away from the optical detection. The above process will be continued until all colorimetric cups have completed the above operation, and this set of delay detection is completed; it is necessary to measure a set of delays at a distance of 15 cup positions. Similarly, each colorimetric cup will stop at a distance of 15 cup positions away from the optical detection. After the same process is carried out, another set of delays will be obtained. Both sets of delays are stored in the system. Under normal circumstances, the delay of the uniform speed segment will be used. Only when the identified photometric point is in the acceleration stage, the delay of the acceleration stage will be used. The jumping photometric points can be directly identified according to the layout. By setting which photometric points are jumping in the software, the software can automatically operate during the operation, and the setting method is the same for the inner and outer circles.

[0033] In this embodiment, in the double-circle system, if the distance between the 6th stage cleaning position of the outer ring W6 and the optical detection position is only the first distance, then when each cuvette blank test is performed, the current cuvette position is in the acceleration stage of the reaction disk rotation; if all the photometric points share a delay point and an unstable AD value is read in the rising edge, two groups of delay operations are set for each cuvette in the outer ring; the delay operation is to detect a group of delay values ​​when each cuvette is at the first distance from the optical detection position, and to detect a group of delay values ​​when each cuvette is at the second distance from the optical detection position, and both groups of delay values ​​are stored in the system. During the test, the outer ring W6 is identified as a beating photometric point and Different delay values ​​are selected to obtain stable AD values; wherein, the first distance is in the acceleration stage of the reaction disk, and the first distance is converted to a first target number of cups according to the acceleration stroke divided by the distance between each colorimetric cup position, and the second distance is in the uniform speed operation stage of the reaction disk, and the second distance is converted to a second target number of cups according to the uniform speed operation stroke divided by the distance between each colorimetric cup position; W6, the 6th stage cleaning position, represents the blank position of each colorimetric cup test cup during the sample test of the fully automatic biochemical analyzer; the number of cups corresponding to the acceleration and deceleration strokes refers to the number of colorimetric cups passed by the reaction disk during the process of accelerating from the initial velocity to the uniform velocity or decelerating from the uniform velocity to stopping.

[0034] Specifically, the absorbance calculation formula is as follows: absorbance of the current colorimetric cup = -2*log (AD value of the current photometric point / AD value of the cup blank), wherein W1-W9 means that each colorimetric cup is subjected to 9-step cleaning after the test is completed to ensure that the colorimetric cup is cleaned and put into use, and W6, the 6th step cleaning, is after cleaning and water injection. During the next rotation process, the optical data of the colorimetric cup will be read. The AD value of the optical data is used to determine whether the current colorimetric cup is cleaned and can be put into use. When it is determined that it can be put into use, reagents and samples are added in the subsequent process for reaction. The AD value of the optical data after the 6th step cleaning read in this process is the cup blank.

[0035] When implemented, the layout of the fully automatic biochemical analyzer is as follows: Figure 4It is a circular layout. Since the cuvettes in the fully automatic biochemical analyzer are repeatedly used, each cuvette will be cleaned after the test is completed. The W1-W9 marked here means that each cuvette must be cleaned in 9 steps before it can be used again after the test is completed. Similarly, it can be understood that before each test starts, the cuvette used needs to be cleaned in 9 steps to ensure that the cuvette is clean and put into use. After W6, the 6th step of cleaning and water filling, the optical data of the cuvette will be read during the next rotation process. The value of this optical data is called AD value to determine whether the cuvette is clean and whether it can be put into use. When it is determined that it can be used, reagents and samples will be added in the subsequent process for reaction. The optical data after 6 steps of cleaning, i.e. AD value, is called cup blank. In addition to determining whether the cuvette can be used, cup blank is also the basis for obtaining absorbance at each subsequent photometric point. Absorbance = -2*log (current photometric point AD / cup blank AD). The absorbance of each photometric point is calculated by this formula, and then the test results are calculated. Each time you clean and fill the colorimetric disc with water, it will stop rotating. After the water filling is completed, it will move to the next position after a fixed time. We read the AD value during the rotation process. Therefore, after W6 is filled with water, the AD value will be read during the next rotation. The positions marked in the left and lower figures show that the outer ring W6 position is less than 2 cup positions away from the optical detection position. At this time, the colorimetric disc is in the acceleration stage of rotation, so the delay point of the acceleration stage is required to read the AD value at this position.

[0036] Based on the same inventive concept, an embodiment of the present invention provides a photometric point calibration system of a fully automatic biochemical analyzer based on a cuvette delay strategy, wherein the fully automatic biochemical analyzer comprises a reaction disk having an inner ring and an outer ring, wherein the movement trajectory of the reaction disk comprises optical detection positions corresponding to the inner ring and the outer ring, wherein the optical detection positions corresponding to the inner ring and the outer ring are spaced apart by N cuvette positions in a clockwise or counterclockwise direction, and wherein the photometric point calibration system of the fully automatic biochemical analyzer comprises: A configuration module, used for configuring the colorimetric cup to be detected; A delay module is used to set multiple different delay times for the same colorimetric cup based on the colorimetric cup delay strategy; The detection module is used to obtain the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and select the optimized delay time to perform delay detection according to the comparison distance result to obtain a stable AD value; During the test, each time the colorimetric disk rotates one circle, each colorimetric cup passes through the optical detection position, and the absorbance data currently read by each colorimetric cup is used as the photometric point. The time point when the photometric point data is read after the optical coupler of the current colorimetric cup sequence is triggered is used as the delay point, and the jumping photometric point is identified and different delay times are selected; Among them, when the colorimetric disk needs to continuously switch between the two states of rotation and stop during the test, acceleration and deceleration operations are performed each time the rotation is started and stopped. During the acceleration and deceleration operation, if there is a sample being tested in the colorimetric cup at the photoelectric detection position, the currently detected photometric point data is the jumping photometric point data.

[0037] It should be noted that the division of the modules in the embodiment of the device / system is only a division of logical functions. In actual implementation, they can be fully or partially integrated into one physical entity, or physically separated. Moreover, these modules can be implemented in the form of software calling through processing elements; or in the form of hardware; or some units can be implemented in the form of software calling through processing elements, and some units can be implemented in the form of hardware.

[0038] The implementation principle of the above modules has been described in the above embodiments, so it will not be repeated here.

[0039] Based on the same inventive concept, in some embodiments of the present application, an electronic device is also provided. The electronic device includes a memory and a processor, wherein the memory is used to store a processing program, and the processor executes the processing program according to instructions. When the processor executes the processing program, the photometric point calibration method of the fully automatic biochemical analyzer based on the cuvette delay strategy in the aforementioned embodiment is implemented.

[0040] In some embodiments of the present application, a readable storage medium is also provided, which can be a non-volatile readable storage medium or a volatile readable storage medium. The readable storage medium stores instructions, and when the instructions are executed on a computer, an electronic device including the readable storage medium executes the aforementioned photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy.

[0041] It is understandable that, for the aforementioned photometric point calibration method of the fully automatic biochemical analyzer based on the cuvette delay strategy, if it is implemented in the form of a software function module and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on such an understanding, the technical solution of the present invention is essentially or the part that contributes to the prior art or the whole or part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including several instructions for a computer device (which can be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the methods of each embodiment of the present invention. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM), random access memory (RAM), disk or optical disk and other media that can store program codes.

[0042] Computer readable storage media may include data signals propagated in baseband or as part of a carrier wave, wherein readable program codes are carried. Such propagated data signals may take various forms, including but not limited to electromagnetic signals, optical signals, or any suitable combination thereof. The readable storage medium may also be any readable medium other than a readable storage medium, which may send, propagate, or transmit a program for use by or in conjunction with an instruction execution system, device, or device. The program codes contained on the readable storage medium may be transmitted using any suitable medium, including but not limited to wireless, wired, optical cable, RF, etc., or any suitable combination thereof.

[0043] The program code involved in executing the technical solution disclosed in this application can be written in any combination of one or more programming languages, including object-oriented programming languages ​​such as Python, C++, etc., and also conventional procedural programming languages ​​such as C language or similar programming languages. The program code can be executed entirely on the user computing device, partially on the user device, as an independent software package, partially on the user computing device and partially on the remote computing device, or entirely on the remote computing device or server. In the case of a remote computing device, the remote computing device can be connected to the user computing device through any type of network, including a local area network (LAN) or a wide area network (WAN), or can be connected to an external computing device (for example, using an Internet service provider to connect through the Internet).

[0044] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A photometric point calibration method for a fully automatic biochemical analyzer based on a cuvette delay strategy, wherein the fully automatic biochemical analyzer comprises a reaction disk having an inner ring and an outer ring, wherein the movement trajectory of the reaction disk comprises optical detection positions corresponding to the inner ring and the outer ring, wherein the optical detection positions corresponding to the inner ring and the outer ring are spaced apart by N cuvette positions in a clockwise or counterclockwise direction, wherein: The photometric point calibration steps of the fully automatic biochemical analyzer are as follows: Configure the cuvette to be tested; Based on the cuvette delay strategy, multiple different delay times are set for the same cuvette; Obtain the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and select the optimized delay time for delay detection according to the comparison distance result to obtain a stable AD value; During the test, each time the colorimetric disk rotates one circle, each colorimetric cup passes through the optical detection position, and the absorbance data currently read by each colorimetric cup is used as the photometric point. The time point when the photometric point data is read after the optical coupler of the current colorimetric cup sequence is triggered is used as the delay point, and the jumping photometric point is identified and different delay times are selected; Among them, when the colorimetric disk needs to continuously switch between the two states of rotation and stop during the test, acceleration and deceleration operations are performed each time the rotation is started and stopped. During the acceleration and deceleration operation, if there is a sample being tested in the colorimetric cup at the photoelectric detection position, the currently detected photometric point data is the jumping photometric point data.

2. The photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as claimed in claim 1, characterized in that: The method of setting a plurality of different delay times for the same cuvette based on the cuvette delay strategy further includes: Configure the first target distance from the optical detection position to the first reading delay of the first sequence number cuvette; Configure the second target distance from the optical detection position for the second reading delay of the second numbered cuvette; Configure the Nth target distance from the optical detection position to the Nth reading delay of the Nth numbered cuvette; Among them, the first target distance, the second target distance and the Nth target distance are all target distances between the colorimetric cup and the optical detection position when the reaction disk is in a stopped state, and the target distances enable the reaction disk to have different distances in the acceleration stage, deceleration stage and uniform speed stage to match different delays.

3. The photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as claimed in claim 1, characterized in that: The method of obtaining the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and selecting an optimized delay time for delay detection according to the distance comparison result to obtain a stable AD value includes: If the delay of all cuvettes being away from the optical detection target distance is detected, the cuvette with the current serial number is placed at a position away from the optical detection position target distance and then rotated; During the rotation process, all AD data of the colorimetric cup with the current serial number are read to form a delay curve; Acquire a stable target point as a delay point based on the delay curve; The next numbered cuvette is placed at a position at a target distance from the optical detection position and the above process is continued until all cuvettes are traversed to complete a set of delayed detection; According to the above operation process, the motion parameters of the instrument are obtained to calculate the distance of the colorimetric cup from the optical detection position in the acceleration stage and the deceleration stage, so as to identify the jumping photometric point and select the corresponding delay time; The target distance includes the relative distance between the colorimetric cup and the optical detection position when the reaction disk is in the acceleration stage and the reaction disk is in the uniform speed operation stage.

4. The photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as claimed in claim 1, characterized in that: The method of obtaining the motion parameters of the instrument according to the above operation process and calculating the distance between the colorimetric cup and the optical detection position in the acceleration stage and the deceleration stage includes: Obtain the operating parameters of the fully automatic biochemical analyzer configuration; Calculate the colorimetric cup distance optical detection position corresponding to the acceleration stage and convert it into the corresponding colorimetric cup position number C=((V1+V2)*T1*α*m) / 720*n*i; wherein the operating parameters include initial velocity V1, uniform velocity V2, motor subdivision n, motor step distance α, reaction disk rotation transmission ratio i, reaction disk colorimetric cup number m, and 720 is a constant for angle calculation.

5. The photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as claimed in claim 1, characterized in that: Under normal circumstances, the first delay time is configured as the uniform speed stage. If the identified photometric point is in the acceleration stage, the second delay time is configured as the acceleration stage. According to the instrument layout, the jumping photometric point is directly identified, and a different third delay time is selected and configured.

6. The photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as claimed in claim 1, characterized in that: In the double-circle system, if the distance between the 6th stage cleaning position of the outer circle W6 and the optical detection position is only the first distance, the current cuvette position is in the acceleration stage of the reaction disk rotation during each cuvette blank test; If all the photometric points share a delay point and unstable AD values ​​are read during the rising edge, two groups of delay operations are set for each colorimetric cup in the outer circle; The delay operation is to detect a set of delay values ​​when each colorimetric cup is at a first distance from the optical detection position, and to detect a set of delay values ​​when each colorimetric cup is at a second distance from the optical detection position, and both sets of delay values ​​are stored in the system. During the test, the outer ring W6 is identified as a beating photometric point and different delay values ​​are selected to obtain a stable AD value; Among them, the first distance is in the acceleration stage of the reaction disk, and the first distance is converted to the first target number of cups according to the acceleration stroke divided by the distance between each cuvette cup position, and the second distance is in the uniform speed operation stage of the reaction disk, and the second distance is converted to the second target number of cups according to the uniform speed operation stroke divided by the distance between each cuvette cup position; W6 the 6th stage cleaning position represents the blank position of each cuvette test cup during the sample test of the fully automatic biochemical analyzer; the number of cups corresponding to the acceleration and deceleration strokes refers to the number of cuvettes passed by the reaction disk during the process of accelerating from the initial speed to the uniform speed or decelerating from the uniform speed to stopping.

7. The photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as claimed in claim 6, characterized in that: The absorbance calculation formula is as follows: absorbance of the current cuvette = -2*log (AD value of the current photometric point / AD value of the cup blank), wherein W1-W9 means that each cuvette is subjected to 9-step cleaning after the test is completed to ensure that the cuvette is clean and put into use, and W6, the 6th step cleaning, is after cleaning and water injection. During the next rotation process, the optical data of the cuvette will be read. The AD value of the optical data is used to determine whether the current cuvette is clean and can be put into use. When it is determined that it can be put into use, reagents and samples are added in the subsequent process for reaction. The AD value of the optical data after the 6th step cleaning read in this process is the cup blank.

8. A photometric point calibration system for a fully automatic biochemical analyzer based on a cuvette delay strategy, the fully automatic biochemical analyzer comprising a reaction disk having an inner ring and an outer ring, the movement trajectory of the reaction disk having optical detection positions corresponding to the inner ring and the outer ring, the optical detection positions corresponding to the inner ring and the outer ring being spaced apart by N cuvette positions in a clockwise or counterclockwise direction, characterized in that: The photometric point calibration system of the fully automatic biochemical analyzer comprises: A configuration module, used for configuring the colorimetric cup to be detected; A delay module is used to set multiple different delay times for the same colorimetric cup based on the colorimetric cup delay strategy; The detection module is used to obtain the distance between the photometric point of the current colorimetric cup and the optical detection position during detection, and select the optimized delay time for delay detection according to the comparison distance result to obtain a stable AD value; During the test, each time the colorimetric disk rotates one circle, each colorimetric cup passes through the optical detection position, and the absorbance data currently read by each colorimetric cup is used as the photometric point. The time point when the photometric point data is read after the optical coupler of the current colorimetric cup sequence is triggered is used as the delay point, and the jumping photometric point is identified and different delay times are selected; Among them, when the colorimetric disk needs to continuously switch between the two states of rotation and stop during the test, acceleration and deceleration operations are performed each time the rotation is started and stopped. During the acceleration and deceleration operation, if there is a sample being tested in the colorimetric cup at the photoelectric detection position, the currently detected photometric point data is the jumping photometric point data.

9. An electronic device, characterized in that: include: A memory, the memory being used to store a processing program; A processor, wherein when executing the processing program, the processor implements the photometric point calibration method of a fully automatic biochemical analyzer based on a cuvette delay strategy as described in any one of claims 1 to 7.

10. A readable storage medium, characterized in that: The readable storage medium stores a processing program, and when the processing program is executed by the processor, the photometric point calibration method of the fully automatic biochemical analyzer based on the cuvette delay strategy as claimed in any one of claims 1 to 7 is implemented.

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