Formula of health-care powder for assisting in repairing damaged cells

Abstract

The invention provides a health-care powder formula for assisting in repairing damaged cells, and belongs to the field of health-care food, the health-care powder formula for assisting in repairing damaged cells comprises the following components in percentage by weight: 15% of sea cucumber peptide powder, 15% of bovine colostrum powder and 70% of bovine bone marrow polypeptide powder; the preparation method of the sea cucumber peptide powder comprises the following steps: selecting fresh sea cucumbers, cleaning the sea cucumbers, and treating the sea cucumbers by adopting an enzymolysis technology under the conditions that the pH value is 7.0-8.0, the temperature is 45-55 DEG C and the time is 6-8 hours; after the enzymolysis is finished, macromolecular substances are removed by adopting a centrifugal separation method, the centrifugal condition is 3000rpm, and the time is 20 minutes; collecting supernate, further purifying through dialysis or an ultrafiltration technology, and finally preparing sea cucumber peptide powder by adopting an ultra-low temperature freeze-drying method. The health-care powder contains sea cucumber polypeptide powder, bovine colostrum powder and bovine bone marrow polypeptide powder which are known components beneficial to body health.

Description

Technical Field

[0001] The invention belongs to the field of health food, and in particular relates to a health powder formula for assisting in repairing damaged cells. Background Art

[0002] As people's living standards continue to improve and their health awareness grows, more and more people are beginning to pay attention to their own health care, especially the growing demand for cell damage repair. Cell damage can be caused by a variety of factors, including but not limited to environmental pollution, radiation, chemical toxin exposure, bad living habits, etc. Long-term cell damage may lead to the occurrence and development of various diseases, so finding an effective cell repair method has become an important topic.

[0003] There are many health products on the market that claim to have the function of repairing damaged cells, but their effects often vary from person to person and lack scientific support. Traditional cell repair methods mainly rely on drug treatment, but drugs usually have certain side effects and are not suitable for everyone. Therefore, it is particularly urgent to develop a safe and effective cell repair health product based on natural ingredients.

[0004] In recent years, studies have shown that certain peptides from animals have good biological activity for the human body and can promote cell growth and repair. Among them, sea cucumbers have attracted much attention due to their unique physiological structure and rich biologically active substances; bovine colostrum is believed to have the effect of enhancing immunity and promoting cell repair because it contains rich immune factors and growth factors; bovine bone marrow is believed to provide essential amino acids and help cell regeneration and repair because it is rich in protein and other nutrients.

[0005] However, it is still a technical challenge to effectively combine these ingredients and ensure their bioavailability and safety. Existing technical means may cause partial activity loss in the process of extracting, purifying and maintaining these active ingredients, which in turn affects the effect of the final product. Therefore, it is necessary to develop a new preparation method that can achieve the optimal ratio while maintaining the activity of each component to achieve effective repair of damaged cells. Summary of the invention

[0006] The purpose of the present invention is to provide a health powder formula for assisting in repairing damaged cells, aiming to solve the problem that the traditional repair methods in the prior art may have a limited mechanism of action of a single ingredient, resulting in low efficiency of cell repair. In addition, many existing cell repair health products have ingredients that are difficult to be fully absorbed and utilized by the human body, thus limiting their efficacy.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] The health care powder for assisting in repairing damaged cells is characterized in that the health care powder comprises the following components in percentage by weight: 15% sea cucumber peptide powder, 15% bovine colostrum powder, and 70% bovine bone marrow polypeptide powder.

[0009] As a preferred solution of the present invention, the sea cucumber peptide powder is prepared by enzymatic hydrolysis, separation, purification, drying and other steps of fresh sea cucumber.

[0010] As a preferred solution of the present invention, the bovine colostrum powder is prepared by ultra-low temperature freeze-drying process of colostrum obtained from healthy cows within three days after giving birth.

[0011] As a preferred solution of the present invention, the bovine bone marrow polypeptide powder is prepared from fresh bovine bone marrow through the steps of enzymatic hydrolysis, separation, concentration, drying and the like.

[0012] As a preferred solution of the present invention, the particle size of the health powder is less than or equal to 200 microns.

[0013] As a preferred solution of the present invention, the preparation method of the health-care powder for assisting in repairing damaged cells specifically comprises the following steps:

[0014] (a) extracting sea cucumber peptide powder;

[0015] (b) preparing bovine colostrum powder;

[0016] (c) preparing bovine bone marrow polypeptide powder;

[0017] (d) mixing the components in the proportions described in claim 1;

[0018] (e) Check the quality of the mixture and package it.

[0019] As a preferred solution of the present invention, the enzymatic hydrolysis process in step (a) is carried out at a pH value of 6 to 8.

[0020] As a preferred solution of the present invention, the ultra-low temperature freeze drying process in step (b) is a process of freezing the material at a very low temperature and directly converting the water from solid to gas by reducing pressure to achieve drying. The product must be pre-frozen before drying, and the pre-freezing temperature should be 10°C to 20°C lower than the eutectic point. Using low temperature and vacuum technology, a refrigeration temperature of -80 to -150°C is provided for stabilization and dehydration treatment and storage.

[0021] As a preferred solution of the present invention, the concentration process in step (c) is carried out under vacuum conditions, and the concentration temperature does not exceed 50°C.

[0022] As a preferred solution of the present invention, the mixing process in step (d) is carried out in an environment with a temperature below 25° C. and a relative humidity below 40%.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. In this solution: the health powder contains sea cucumber peptide powder, bovine colostrum powder and bovine bone marrow polypeptide powder, which are known to be beneficial to health. Sea cucumber peptide powder and bovine bone marrow polypeptide powder are rich in small molecule peptides that are easy to absorb, which can provide the body with necessary amino acids, promote protein synthesis, and help cell repair and regeneration. Bovine colostrum powder is rich in immunoglobulins and growth factors, which can enhance the body's immunity and promote the recovery of damaged tissues.

[0025] 2. In this scheme: special attention is paid to the processing method of ingredients in the production process of health powder, such as the application of technologies such as enzymatic hydrolysis and ultra-low temperature freeze drying, so that the particle size of the final product is controlled below 200 microns, and the product is processed immediately after cooling, separated and defatted, sterilized, concentrated, and freeze-sublimated to fully ensure the activity of immunoglobulin. This is conducive to the absorption of nutrients by the human digestive system, thereby improving bioavailability. At the same time, the environmental conditions are controlled during the mixing process (temperature below 25°C, relative humidity below 40%) to ensure the stability of the product during processing and avoid degradation of active ingredients due to high temperature and humidity.

[0026] 3. In this plan: strict quality control is carried out in every step from the selection of raw materials to the production process. For example, the enzymatic hydrolysis process of sea cucumber peptide powder is carried out under suitable pH conditions, the ultra-low temperature freeze-drying process of bovine colostrum powder is completed at a specific temperature, and the concentration process of bovine bone marrow polypeptide powder is operated under low temperature vacuum conditions. These measures ensure the safety and effectiveness of the final product. In addition, there will be a special quality inspection link after the preparation is completed to ensure that each batch of products can meet the predetermined quality standards.

[0027] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0028] Figure 1 This is a test chart of related indicators in blood of the present invention;

[0029] Figure 2 This is a graph showing the cell survival rate test of the present invention;

[0030] Figure 3 This is a graph showing the cell viability test of the present invention. DETAILED DESCRIPTION

[0031] Example 1

[0032] The present invention provides the following technical solutions:

[0034] This embodiment proposes a health powder for assisting in repairing damaged cells, characterized in that the health powder comprises the following components in weight percentage: 15% sea cucumber peptide powder, 15% bovine colostrum powder, and 70% bovine bone marrow polypeptide powder.

[0035] The preparation method of sea cucumber peptide powder specifically comprises the following steps:

[0036] 1. Select fresh sea cucumbers, clean them, and use enzymatic hydrolysis technology. The enzymatic hydrolysis conditions are pH 7.0-8.0, temperature 45-55°C, and time for 6-8 hours;

[0037] 2. After the enzymatic hydrolysis, the macromolecular substances were removed by centrifugation at 3000 rpm for 20 minutes;

[0038] 3. Collect the supernatant, further purify it through dialysis or ultrafiltration technology, and finally obtain sea cucumber peptide powder by ultra-low temperature freeze-drying method.

[0039] The preparation method of bovine colostrum powder specifically comprises the following steps:

[0040] 1. Select colostrum from healthy cows on the first day after giving birth, and pasteurize it at 72°C for 15 seconds;

[0041] 2. The sterilized colostrum is prepared into powder by freeze drying.

[0042] The method for preparing bovine bone marrow polypeptide powder specifically comprises the following steps:

[0043] (1) fresh bovine bone marrow is selected, cleaned, and treated with enzymatic hydrolysis technology, wherein the enzymatic hydrolysis conditions are pH 6.0-7.0, temperature 50-60° C., and time 4-6 hours;

[0044] (2) After the enzymatic hydrolysis, the macromolecular substances were removed by centrifugation at 4000 rpm for 25 min.

[0045] (3) collecting the supernatant, further purifying it through dialysis or ultrafiltration technology, and finally obtaining bovine bone marrow polypeptide powder through ultra-low temperature freeze-drying method.

[0046] This embodiment provides a method for preparing a health-care powder for assisting in repairing damaged cells, which specifically comprises the following steps:

[0047] S1. Prepare sea cucumber peptide powder, bovine colostrum powder and bovine bone marrow polypeptide powder respectively according to the above method;

[0048] S2. Mix the three powders evenly according to the ratio of 15% sea cucumber peptide powder, 15% bovine colostrum powder and 70% bovine bone marrow polypeptide powder;

[0049] S3. Check the quality of the mixed health powder to ensure that there is no caking;

[0050] S4. Use appropriate packaging materials for sealed packaging to ensure the shelf life of the product.

[0051] Example 2

[0052] This embodiment proposes a health powder for assisting in repairing damaged cells, characterized in that the health powder comprises the following components in weight percentage: 15% sea cucumber peptide powder, 15% bovine colostrum powder, and 70% bovine bone marrow polypeptide powder.

[0053] The preparation method of sea cucumber peptide powder, bovine colostrum powder and bovine bone marrow polypeptide powder in this embodiment is the same as that in embodiment 1, except that:

[0054] S1. Prepare sea cucumber peptide powder, bovine colostrum powder and bovine bone marrow polypeptide powder respectively according to the above method;

[0055] S2, after mixing the above three powders, perform microwave sterilization, the sterilization conditions are microwave power 600W, and the treatment time is 3 minutes;

[0056] S3. Check the quality of the sterilized health powder to ensure that there is no caking;

[0057] S4. Use appropriate packaging materials for sealed packaging to ensure the shelf life of the product.

[0058] Experimental Example 1

[0059] Bioavailability test

[0060] Test sample: the health powder for assisting in repairing damaged cells prepared in Example 1-2.

[0061] Test method: The samples are orally administered to animal models (such as mice) to observe their effects on cell repair and to evaluate bioavailability through changes in relevant indicators in the blood.

[0062] Experimental Example 2

[0063] Safety Test

[0064] Test sample: the health powder for assisting in repairing damaged cells prepared in Example 1-2.

[0065] Test method: Subject the samples to acute toxicity tests and long-term toxicity tests to observe whether there are any toxic side effects and evaluate their safety.

[0066] Experimental Example 3

[0067] Functional experiments

[0068] Test sample: the health powder for assisting in repairing damaged cells prepared in Example 1-2.

[0069] Test method: The samples are given to cell damage models (such as oxidative stress damage cell models) to observe their repair effects on cell damage, and the functionality is evaluated by changes in indicators such as cell survival rate and cell proliferation ability.

[0070] Comparative Example 1

[0071] This comparative example provides a health-care powder for assisting in repairing damaged cells. The difference between the health-care powder and Example 1 is that the health-care powder does not contain sea cucumber peptide powder. The preparation method of the health-care powder is the same as that of Example 1.

[0072] Comparative Example 2

[0073] This comparative example provides a health-care powder for assisting in repairing damaged cells. The difference between the health-care powder and Example 1 is that the health-care powder does not contain bovine colostrum powder. The preparation method of the health-care powder is the same as that of Example 1.

[0074] Comparative Example 3

[0075] This comparative example provides a health-care powder for assisting in repairing damaged cells. The difference between the health-care powder and Example 1 is that the health-care powder does not contain bovine bone marrow polypeptide powder. The preparation method of the health-care powder is the same as that of Example 1.

[0076] Experimental Results

[0077] The above experimental results show that the bioavailability, safety and functionality of Examples 1-2 of the present invention are significantly better than those of Comparative Examples 1-3, indicating that the health powder of the present invention has good bioavailability, high safety and significant functional effects.

[0078] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A health powder for assisting in repairing damaged cells, characterized in that: The health powder comprises the following components in percentage by weight: 15% sea cucumber peptide powder, 15% bovine colostrum powder, and 70% bovine bone marrow polypeptide powder.

2. The health-care powder for assisting in repairing damaged cells according to claim 1, characterized in that: The sea cucumber peptide powder is prepared from fresh sea cucumbers through the steps of enzymolysis, separation, purification, drying and the like.

3. The health-care powder for assisting in repairing damaged cells according to claim 1, characterized in that: The bovine colostrum powder is prepared from colostrum within three days after delivery of a healthy cow through an ultra-low temperature freeze-drying process.

4. The health-care powder for assisting in repairing damaged cells according to claim 1, characterized in that: The bovine bone marrow polypeptide powder is prepared from fresh bovine bone marrow through the steps of enzymolysis, separation, concentration, drying and the like.

5. The health-care powder for assisting in repairing damaged cells according to any one of claims 1 to 4, characterized in that: The particle size of the health powder is less than or equal to 200 microns.

6. The health-care powder for assisting in repairing damaged cells according to claim 1, characterized in that: The preparation method of the health-care powder for assisting in repairing damaged cells specifically comprises the following steps: (a) extracting sea cucumber peptide powder; (b) preparing bovine colostrum powder; (c) preparing bovine bone marrow polypeptide powder; (d) mixing the components in the proportions described in claim 1; (e) Check the quality of the mixture and package it.

7. The health-care powder for assisting in repairing damaged cells according to claim 6, characterized in that: The enzymatic hydrolysis process in step (a) is carried out at a pH value of 6 to 8.

8. The health-care powder for assisting in repairing damaged cells according to claim 6, characterized in that: The ultra-low temperature freeze drying process in step (b) is a process in which the material is frozen at a very low temperature and the water is directly converted from solid to gas by means of decompression, thereby achieving drying. The product must be pre-frozen before drying, and the pre-freezing temperature should be 10°C to 20°C lower than the eutectic point. Using low temperature and vacuum technology, a refrigeration temperature of -80 to -150°C is provided for stabilization and dehydration treatment and storage.

9. The health-care powder for assisting in repairing damaged cells according to claim 6, characterized in that: The concentration process in step (c) is carried out under vacuum conditions, and the concentration temperature does not exceed 50°C.

10. The health-care powder for assisting in repairing damaged cells according to claim 6, characterized in that: The mixing process in step (d) is carried out in an environment with a temperature below 25° C. and a relative humidity below 40%.

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