Precipitation and filtration method for bacterial liquid collection

Abstract

The invention belongs to the technical field of biological medicine equipment, and particularly relates to a precipitation and filtration method for bacterial liquid collection, which comprises the following steps: S1, sterilizing and drying a precipitation and filtration device to reduce the influence of infectious microbes on bacterial liquid; s2; adding the bacterial liquid needing to be filtered and precipitated into the settling cylinder, and standing; s3, placing a collecting cup in a placing groove, slowly and upwards pushing an ejector pin, pushing the bacterial liquid after standing to a flow dividing pipe, enabling the bacterial liquid to flow out from the flow dividing pipe, and collecting the bacterial liquid in the collecting cup; and S4, stopping pushing the ejector pin when one third of the bacterial liquid is left, storing the collecting cup, and properly treating the residual bacterial liquid, so as to complete the precipitation and filtration of the bacterial liquid.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical equipment, and in particular is a precipitation filtering method for collecting bacterial liquid. Background Art

[0002] When culturing bacteria, the mother bacteria needs to be placed in a culture medium for cultivation. When the bacteria grow too vigorously, the culture medium will become yellow and turbid, accompanied by the formation of sediment. In order to reduce the impact of the sediment on the experiment, a filtration device is required to separate the sediment from the bacterial solution.

[0003] The existing technologies for separating bacterial liquid from precipitate include centrifugation, filtration, precipitation promotion, gravity sedimentation, etc. When selecting a separation method, the density, size, volume and biological activity of the precipitate need to be considered.

[0004] Because the equipment needs to be sterilized before sedimentation and filtration of the bacterial solution to reduce the impact of miscellaneous bacteria on the bacterial solution, the traditional equipment is large in size, which makes it inconvenient to sterilize the equipment. Therefore, a sedimentation and filtration method for collecting bacterial solution is proposed to address the above problem. Summary of the invention

[0005] In order to make up for the deficiencies of the prior art and solve at least one technical problem raised in the background technology, the present invention proposes a precipitation filtration method for collecting bacterial liquid.

[0006] The technical solution adopted by the present invention to solve the technical problem is: a precipitation filtration method for collecting bacterial liquid according to the present invention comprises the following steps:

[0007] S1: Sterilize and dry the sedimentation and filtration device to reduce the impact of miscellaneous bacteria on the bacterial solution;

[0008] S2: adding the bacterial solution to be filtered and precipitated into the sedimentation cylinder and letting it stand;

[0009] S3: Place the collection cup in the placement tank, slowly push the ejector pin upwards, and push the static bacterial solution to the shunt tube, so that the bacterial solution flows out of the shunt tube and is collected inside the collection cup;

[0010] S4: When one third of the bacterial liquid remains, stop pushing the ejector pin, collect the collection cup and properly dispose of the residual bacterial liquid, thereby completing the sedimentation and filtration of the bacterial liquid.

[0011] Preferably, the sedimentation and filtration device proposed in S1 includes a sedimentation table; a placement groove is provided on the top of the sedimentation table; a collecting cup is provided on the inner wall of the placement groove; a fixing frame is fixedly connected to the side wall of the sedimentation table; a thread is provided on the inner wall of the fixing frame; a sedimentation cylinder is threadedly connected to the inner wall of the fixing frame; a diversion pipe is fixedly connected to the side wall of the sedimentation cylinder; a push pin is connected to the inner wall of the sedimentation cylinder by interference fit; and a push plate is provided at the bottom of the push pin.

[0012] Preferably, a rotating groove is provided on the side wall of the settling platform; a support frame is rotatably connected to the inner side wall of the rotating groove; a funnel is installed in the middle of the support frame; and anti-collision cotton is fixedly connected to the side wall of the support frame.

[0013] Preferably, the inner side wall of the placement groove is provided with a plurality of sliding grooves; the inner side wall of the sliding groove is fixedly connected with a first magnet; the inner side wall of the sliding groove is slidably connected with a second magnet; and the side wall of the second magnet is fixedly connected with a sliding extrusion block.

[0014] Preferably, a gasket is installed at the bottom of the inner side wall of the placement groove; the bottom of the inner side wall of the placement groove is opened and closed with multiple groups of drainage holes; the side wall of the gasket is opened and closed with multiple groups of drainage holes; the drainage holes are arranged in a circular array.

[0015] Preferably, a filter is provided on the inner side wall of the diversion pipe; the filter is slidably connected to the inner side wall of the diversion pipe; and two pairs of rubber pads are fixedly connected to the bottom of the settling platform.

[0016] Preferably, the side wall of the sliding extrusion block is provided with an anti-skid pad; the anti-skid pad is fixedly connected to the side wall of the sliding extrusion block; and the anti-skid pad is in contact with the side wall of the collection cup.

[0017] Preferably, the ejector pin and the push plate are connected via threads; the threads between the ejector pin and the push plate are reverse threads.

[0018] Beneficial effects of the present invention:

[0019] The present invention provides a precipitation filtration method for collecting bacterial liquid. By setting a separation device, the cost of the equipment is effectively reduced compared with the separation of a centrifugal device. At the same time, the equipment has the advantages of small size, easy storage and low cost. The small size is not only convenient for staff to store the equipment, but also convenient for staff to sterilize and disinfect the equipment. After using the equipment, the staff can move the equipment to a cleaning pool for cleaning, thereby improving the convenience of the equipment.

[0020] The present invention provides a sedimentation filtration method for collecting bacterial liquid. A support frame is arranged on the top of the collection cup, and the support frame can move left and right. A funnel is placed in the middle of the support frame. When using the equipment for solid-liquid separation, the staff places filter paper inside the funnel, and then moves the support frame to just below the end of the shunt pipe. The liquid flows out from the shunt pipe and flows into the funnel. After being filtered by the filter paper, it drips into the collection cup. The secondary filtration is arranged to further reduce the content of bacterial liquid sediment, thereby improving the accuracy of bacterial liquid data during detection. At the same time, anti-collision cotton is fixedly connected to the side wall of the support frame. When the anti-collision cotton is moved to the side wall of the sedimentation platform, it can play a buffering role to protect the movement of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The drawings described herein are used to provide further understanding of the present invention and constitute a part of this application. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0022] In the attached picture:

[0023] Figure 1 is a flow chart of the method of the present invention;

[0024] Figure 2 is a stereogram of the present invention;

[0025] Figure 3 is a stereogram of the present invention;

[0026] Figure 4 is a cross-sectional view of the settling platform of the present invention;

[0027] Figure 5 for Figure 4 A in the enlarged view;

[0028] Figure 6 is a three-dimensional diagram of the rubber pad of the present invention;

[0029] Figure 7 is a three-dimensional diagram of the support frame and the funnel in the present invention;

[0030] Figure 8 is a cross-sectional view of a sedimentation cylinder in the present invention;

[0031] Fig. 9 for Figure 8 The enlarged view of point B in the figure;

[0032] Fig.10 for Figure 8 The enlarged view of point C in the figure;

[0033] Legend:

[0034] 1. Sedimentation table; 11. Placement trough; 12. Collecting cup; 13. Fixed frame; 14. Sedimentation cylinder; 15. Diversion pipe; 16. Ejector pin; 17. Push plate; 2. Rotating trough; 21. Support frame; 22. Funnel; 23. Anti-collision cotton; 3. Sliding trough; 31. First magnet; 32. Second magnet; 33. Sliding extrusion block; 4. Gasket; 41. Drain hole; 5. Rubber pad; 6. Filter; 7. Anti-slip pad. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Specific examples are given below.

[0037] See also Figure 1-Figure 10 The present invention provides a precipitation filtration method for collecting bacterial liquid, comprising the following steps:

[0038] S1: Sterilize and dry the sedimentation and filtration device to reduce the impact of miscellaneous bacteria on the bacterial solution;

[0039] S2: Add the bacterial solution to be filtered and precipitated to the sedimentation cylinder 14 and let it stand;

[0040] S3: placing the collection cup 12 in the placement tank 11, slowly pushing the ejector pin 16 upward, pushing the static bacterial solution to the shunt tube 15, so that the bacterial solution flows out from the shunt tube 15 and is collected in the collection cup 12;

[0041] S4: When one third of the bacterial liquid remains, the push pin 16 is stopped, and the collection cup 12 is stored and the residual bacterial liquid is properly disposed of, thereby completing the sedimentation and filtration of the bacterial liquid.

[0042] Further, such as Figure 1-Figure 10As shown, the sedimentation and filtration device proposed in S1 includes a sedimentation platform 1; a placement groove 11 is provided on the top of the sedimentation platform 1; a collecting cup 12 is provided on the inner wall of the placement groove 11; a fixing frame 13 is fixedly connected to the side wall of the sedimentation platform 1; a thread is provided on the inner wall of the fixing frame 13; a sedimentation cylinder 14 is threadedly connected to the inner wall of the fixing frame 13; a shunt pipe 15 is fixedly connected to the side wall of the sedimentation cylinder 14; a thimble 16 is connected to the inner wall of the sedimentation cylinder 14 by interference fit; the bottom of the thimble 16 A push plate 17 is provided. When working, the staff first adds the bacterial solution to be precipitated and filtered into the sedimentation cylinder 14 in the container, that is, a layer of filter paper is laid on the surface of the ejector pin 16, and then the ejector pin 16 is pulled to the bottom of the sedimentation cylinder 14 to add the bacterial solution to be precipitated and filtered into the sedimentation cylinder 14. Then, the sedimentation cylinder 14 is fixed in the fixing frame 13 by threads, and the push plate 17 is fixed to the end of the ejector pin 16. At the same time, the shunt tube 15 is rotated to the top of the collection cup 12, and then it is left to stand. The bacterial liquid is allowed to settle to the bottom under the action of gravity. When the sediment is settled at the bottom of the sedimentation cylinder 14, the push plate 17 is slowly pushed upward. At this time, the ejector pin 16 slowly moves upward inside the sedimentation cylinder 14. When the bacterial liquid moves to the part where the shunt pipe 15 is connected to the sedimentation cylinder 14, the bacterial liquid will flow out from the shunt pipe 15 and then flow into the collection cup 12. As the ejector pin 16 rises, the bacterial liquid will continue to flow from the sedimentation cylinder 14 to the collection cup 12. The last part of the accompanying sediment and the bacterial liquid will not be used, thereby completing the separation of the bacterial liquid and the sediment. By setting the separation device, compared with the separation of the centrifugal device, the cost of the equipment is effectively reduced. At the same time, the equipment has the advantages of small size, easy storage, and low cost. The small size is not only convenient for the staff to store the equipment, but also convenient for the staff to sterilize and disinfect the equipment. After using the equipment, the staff can move the equipment to the cleaning pool for cleaning, thereby improving the convenience of the equipment.

[0043] Further, such as Figure 1-Figure 10As shown, the side wall of the settling platform 1 is provided with a rotating groove 2; the inner side wall of the rotating groove 2 is rotatably connected to a support frame 21; a funnel 22 is installed in the middle of the support frame 21; the side wall of the support frame 21 is fixedly connected with an anti-collision cotton 23. When working, when solid-liquid separation is performed, a small amount of material will remain in the liquid. By setting the support frame 21 on the top of the collection cup 12, and the support frame 21 can move left and right, and placing the funnel 22 in the middle of the support frame 21, when using the equipment for solid-liquid separation, the staff will place the filter paper on the funnel. The support frame 21 is moved to the inside of the funnel 22, and then the support frame 21 is moved to the bottom of the end of the shunt pipe 15. The liquid flows out from the shunt pipe 15, flows into the inside of the funnel 22, and is filtered by the filter paper and drips into the inside of the collecting cup 12. The secondary filtration is set to further reduce the content of the bacterial liquid sediment, thereby improving the accuracy of the bacterial liquid data during detection. At the same time, the side wall of the support frame 21 is fixedly connected with anti-collision cotton 23. When the anti-collision cotton 23 is moved to the side wall of the sedimentation platform 1, it can play a buffering role to protect the movement of the equipment.

[0044] Further, such as Figure 1-Figure 10 As shown, the inner wall of the placement groove 11 is provided with multiple groups of sliding grooves 3; the inner wall of the sliding groove 3 is fixedly connected with a first magnet 31; the inner wall of the sliding groove 3 is slidably connected with a second magnet 32; the side wall of the second magnet 32 ​​is fixedly connected with a sliding extrusion block 33. During operation, when the collecting cup 12 is placed inside the placement groove 11, the collecting cup 12 will squeeze the sliding extrusion block 33 into the sliding groove 3. Since the second magnet 32 ​​and the first magnet 31 are made of the same material and there is a repulsive force between the two, under the action of the repulsive force, the first magnet 31 will generate a reaction force on the second magnet 32, thereby pushing the second magnet 32 ​​in the opposite direction. Through the multiple sliding extrusion blocks 33 and the collection cup 12 being squeezed and fixed inside the placement groove 11, the stability of the collection cup 12 is increased. When using a container to collect bacterial liquid, the collection cups 12 of different specifications have different sizes. The setting of the fixing device can effectively fix the collection cups 12 of different sizes, thereby improving the convenience of the equipment.

[0045] Further, such as Figure 1-Figure 10 As shown, a gasket 4 is installed at the bottom of the inner wall of the placement groove 11; a plurality of drainage holes 41 are opened and closed at the bottom of the inner wall of the placement groove 11; a plurality of drainage holes 41 are opened and closed on the side wall of the gasket 4; the drainage holes 41 are arranged in a circular array. During operation, since the collection cup 12 is generally made of glass, the gasket 4 arranged at the bottom of the placement groove 11 effectively protects the collection cup 12 to a certain extent. By setting the drainage holes 41 on the gasket 4 and the bottom of the placement groove 11, when liquid is spilled, the liquid will flow out from the drainage holes 41, thereby reducing the accumulation of liquid inside the placement groove 11, thereby facilitating the cleaning of the equipment by the staff.

[0046] Further, such as Figure 1-Figure 10 As shown, a filter 6 is provided on the inner wall of the shunt pipe 15; the filter 6 is slidably connected to the inner wall of the shunt pipe 15; two pairs of rubber pads 5 are fixedly connected to the bottom of the settling platform 1. During operation, the liquid can be preliminarily filtered by the filter 6 provided at the end of the shunt pipe 15. At the same time, the filter 6 is detachable to facilitate the cleaning of the filter 6 by the staff. The provided filter 6 effectively facilitates the filtering of the liquid by the equipment and improves the filtering effect of the equipment.

[0047] Further, such as Figure 1-Figure 10 As shown, the side wall of the sliding extrusion block 33 is provided with an anti-skid pad 7; the anti-skid pad 7 is fixedly connected to the side wall of the sliding extrusion block 33; the anti-skid pad 7 is in contact with the side wall of the collecting cup 12. During operation, the anti-skid pad 7 is provided, and when the sliding extrusion block 33 is in contact with the side wall of the collecting cup 12, the anti-skid pad 7 is provided, which can effectively increase the friction between the anti-skid pad 7 and the collecting cup 12, thereby improving the fixing effect of the device on the collecting cup 12.

[0048] Further, such as Figure 1-Figure 10 As shown, the ejector pin 16 and the push plate 17 are connected by threads; the threads between the ejector pin 16 and the push plate 17 are reverse threads. During operation, through the detachable part between the push plate 17 and the ejector pin 16, when cleaning the inside of the sedimentation cylinder 14, the staff can disassemble the push plate 17 and then take out the ejector pin 16 from the top of the sedimentation cylinder 14, thereby facilitating the staff to clean the equipment, thereby improving the convenience of the equipment.

[0049] Working principle: When the staff adds the bacterial liquid that needs to be precipitated and filtered into the sedimentation cylinder 14 in the container, that is, a layer of filter paper is laid on the surface of the ejector pin 16, and then the ejector pin 16 is pulled to the bottom of the sedimentation cylinder 14 to add the bacterial liquid that needs to be precipitated and filtered into the sedimentation cylinder 14, and then the sedimentation cylinder 14 is fixed in the fixing frame 13 by threads, and the push plate 17 is fixed to the end of the ejector pin 16. At the same time, the shunt tube 15 is rotated to the top of the collection cup 12, and then the bacterial liquid is allowed to stand to allow the sediment to settle to the bottom under the action of gravity. When the sediment settles to the bottom of the sedimentation cylinder 14, the push plate 17 is slowly pushed upwards. At this time, the ejector pin 16 slowly moves upwards in the sedimentation cylinder 14. When the bacterial liquid moves to the part where the shunt tube 15 and the sedimentation cylinder 14 are connected, the bacterial liquid is filtered out. The liquid will flow out from the shunt pipe 15 and then flow into the collection cup 12. As the ejector pin 16 rises, the bacterial liquid will continue to flow from the sedimentation cylinder 14 into the collection cup 12. The final accompanying sediment and bacterial liquid will not be used, thereby completing the separation of bacterial liquid and sediment. By setting the separation device, compared with the separation of the centrifugal device, the cost of the equipment is effectively reduced. At the same time, the equipment has the advantages of small size, easy storage, and low cost. The small size is not only convenient for the staff to store the equipment, but also convenient for the staff to sterilize and disinfect the equipment. After using the equipment, the staff can move the equipment to the cleaning tank for cleaning, thereby improving the convenience of the equipment. When performing solid-liquid separation, a small amount of substance will remain in the liquid. , by setting a support frame 21 on the top of the collection cup 12, and the support frame 21 can move left and right, and placing the funnel 22 in the middle of the support frame 21, when using the equipment for solid-liquid separation, the staff will place the filter paper inside the funnel 22, and then move the support frame 21 to just below the end of the shunt tube 15. The liquid flows out from the shunt tube 15 and flows into the funnel 22. After being filtered by the filter paper, it drips into the collection cup 12. The secondary filtration is set to further reduce the content of bacterial liquid sediment, thereby improving the accuracy of bacterial liquid data during detection. At the same time, anti-collision cotton 23 is fixedly connected to the side wall of the support frame 21. When the anti-collision cotton 23 is moved to the side wall of the sedimentation platform 1, it can play a buffering role to protect the movement of the equipment. When the collecting cup 12 is placed in the placement groove 11, the collecting cup 12 will squeeze the sliding extrusion block 33 into the sliding groove 3. Since the second magnet 32 ​​and the first magnet 31 are made of the same material and there is repulsion between the two, the first magnet 31 will generate a reaction force on the second magnet 32 ​​under the action of the repulsive force, thereby pushing the second magnet 32 ​​in the opposite direction. The collecting cup 12 is squeezed and fixed in the placement groove 11 by multiple sliding extrusion blocks 33, thereby increasing the stability of the collecting cup 12. When using a container to collect bacterial liquid, the collecting cups 12 of different specifications have different sizes. The fixing device can effectively fix the collecting cups 12 of different sizes, thereby improving the convenience of the equipment. Since the collecting cup 12 is generally made of glass,By setting the gasket 4 at the bottom of the placement groove 11, the collection cup 12 is effectively protected to a certain extent. By setting the drainage hole 41 at the gasket 4 and the bottom of the placement groove 11, when the liquid is spilled, the liquid will flow out from the drainage hole 41, thereby reducing the accumulation of liquid inside the placement groove 11, so as to facilitate the cleaning of the equipment by the staff. By setting the filter 6 at the end of the shunt pipe 15, the liquid can be initially filtered. At the same time, the filter 6 is detachable, so as to facilitate the cleaning of the filter 6 by the staff. The filter 6 is effectively facilitated to filter the liquid by the equipment, and the filtering effect of the equipment is improved. By setting the anti-skid pad 7, when the sliding extrusion block 33 contacts the side wall of the collection cup 12, the anti-skid pad 7 can effectively increase the friction between the anti-skid pad 7 and the collection cup 12, so as to improve the fixing effect of the equipment on the collection cup 12. By the detachable part between the push plate 17 and the ejector pin 16, when cleaning the inside of the sedimentation cylinder 14, the staff can disassemble the push plate 17 and then take out the ejector pin 16 from the top of the sedimentation cylinder 14, so as to facilitate the cleaning of the equipment by the staff, thereby improving the convenience of the equipment. ,

[0050] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A precipitation filtration method for collecting bacterial liquid, characterized in that: The following steps are involved: S1: Sterilize and dry the sedimentation and filtration device to reduce the impact of miscellaneous bacteria on the bacterial solution; S2: adding the bacterial solution to be filtered and precipitated to the sedimentation cylinder (14) and letting it stand; S3: placing the collection cup (12) in the placement tank (11), slowly pushing the ejector pin (16) upwards, and pushing the bacterial liquid after standing to the shunt tube (15), so that the bacterial liquid flows out from the shunt tube (15) and is collected inside the collection cup (12); S4: When one third of the bacterial liquid remains, the push pin (16) is stopped, and the collection cup (12) is collected and the residual bacterial liquid is properly disposed of, thereby completing the sedimentation and filtration of the bacterial liquid.

2. A precipitation filtration method for collecting bacterial liquid according to claim 1, characterized in that: The sedimentation and filtration device proposed in S1 comprises a sedimentation platform (1); a placement groove (11) is provided on the top of the sedimentation platform (1); a collecting cup (12) is provided on the inner wall of the placement groove (11); a fixing frame (13) is fixedly connected to the side wall of the sedimentation platform (1); a thread is provided on the inner wall of the fixing frame (13); a sedimentation cylinder (14) is threadedly connected to the inner wall of the fixing frame (13); a shunt pipe (15) is fixedly connected to the side wall of the sedimentation cylinder (14); a push pin (16) is connected to the inner wall of the sedimentation cylinder (14) by interference fit; a push plate (17) is provided at the bottom of the push pin (16).

3. A precipitation and filtration method for collecting bacterial liquid according to claim 2, characterized in that: The side wall of the settling platform (1) is provided with a rotating groove (2); the inner side wall of the rotating groove (2) is rotatably connected to a support frame (21); a funnel (22) is installed in the middle of the support frame (21); and anti-collision cotton (23) is fixedly connected to the side wall of the support frame (21).

4. A precipitation filtration method for collecting bacterial liquid according to claim 2, characterized in that: The inner wall of the placement groove (11) is provided with a plurality of sliding grooves (3); the inner wall of the sliding groove (3) is fixedly connected with a first magnet (31); the inner wall of the sliding groove (3) is slidably connected with a second magnet (32); the side wall of the second magnet (32) is fixedly connected with a sliding extrusion block (33).

5. A precipitation and filtration method for collecting bacterial liquid according to claim 2, characterized in that: A gasket (4) is installed at the bottom of the inner side wall of the placement groove (11); a plurality of drainage holes (41) are opened and closed at the bottom of the inner side wall of the placement groove (11); a plurality of drainage holes (41) are opened and closed on the side wall of the gasket (4); and the drainage holes (41) are arranged in a circular array.

6. A precipitation and filtration method for collecting bacterial liquid according to claim 2, characterized in that: The inner wall of the diverter pipe (15) is provided with a filter (6); the filter (6) is slidably connected to the inner wall of the diverter pipe (15); and two pairs of rubber pads (5) are fixedly connected to the bottom of the settling platform (1).

7. A precipitation and filtration method for collecting bacterial liquid according to claim 4, characterized in that: The side wall of the sliding extrusion block (33) is provided with an anti-skid pad (7); the anti-skid pad (7) is fixed to the side wall of the sliding extrusion block (33); the anti-skid pad (7) is in contact with the side wall of the collection cup (12).

8. A precipitation and filtration method for collecting bacterial liquid according to claim 2, characterized in that: The ejector pin (16) and the push plate (17) are connected via threads; the threads between the ejector pin (16) and the push plate (17) are reverse threads.

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