Lysozyme extraction and separation equipment

Abstract

The invention relates to the technical field of lysozyme separation and extraction, in particular to lysozyme extraction and separation equipment which comprises a centrifugal table and a separation barrel, an upper barrel cover and a lower barrel cover are mounted on the separation barrel, an L-shaped stirring sheet is mounted on the upper barrel cover, and a separation component is mounted at the bottom of the separation barrel. By means of the device, plant tissue liquid and buffer liquid can be effectively and fully mixed, due to the fact that the L-shaped stirring sheets can ascend and descend in a reciprocating mode, the crushing effect on tissue fragments in the mixture is greatly improved, lysozyme is more fully released by the tissue fragments, more lysozyme is collected, and after centrifugation work is completed, the lysozyme can be effectively separated. The sediment can be stripped into the lower barrel cover by utilizing the separation part, and then the lower barrel cover is screwed off from the threaded butt joint port, so that the sediment can be quickly separated from the separation barrel and collected, and supernatant which is well stood in the upper part of the separation barrel cannot be influenced.

Description

Technical Field

[0001] The invention relates to the technical field of lysozyme separation and extraction, in particular to lysozyme extraction and separation equipment. Background Art

[0002] Lysozyme is an enzyme that is widely present in organisms and has important biological functions. It is widely present in various tissues and secretions of animals, and many plant tissues also contain lysozyme, such as in the roots, stems, leaves, flowers and other organs of plants. It plays a role in the process of plants resisting the invasion of pathogens.

[0003] When lysozyme is extracted from plants, plant tissues rich in lysozyme, such as papaya fruits and spinach leaves, are selected, washed and chopped, and then fully ground in a mortar to break the cells to obtain plant tissue fluid, and then the ground plant tissue fluid is filtered with gauze or filter paper to remove large tissue fragments, and then an appropriate amount of extraction buffer is added to release lysozyme into the buffer, and then the plant tissue fluid and the buffer are centrifuged to obtain a crude extract of plant-derived lysozyme. However, when the current centrifuge separates the crude extract, the plant tissue fluid and the buffer in the reagent tube will be in a static state during the initial start-up of the centrifuge, so that the two cannot be mixed, which affects the separation effect of lysozyme, and after the supernatant and the precipitate are separated in the later stage, part of the precipitate is easily mixed into the supernatant again when the precipitate is separated from the test tube. Summary of the invention

[0004] The purpose of the present invention is to provide a lysozyme extraction and separation device to solve the problem proposed in the above background technology that the plant tissue fluid and buffer solution inside the reagent tube cannot be mixed and that part of the precipitate is easily mixed into the supernatant again when the precipitate is separated from the test tube.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solution: a lysozyme extraction and separation device, comprising a centrifuge table and a separation cylinder mounted on the centrifuge table and centrifugally treating the extract through the centrifuge table, wherein both upper and lower end surfaces of the separation cylinder are open structures, and upper and lower ends of the separation cylinder are respectively installed with an upper cylinder cover and a lower cylinder cover which are threadedly connected and used to seal the end surface openings; The upper cylinder cover is provided with a plurality of L-shaped stirring sheets distributed in an annular manner, through which the extract is stirred and crushed, and the bottom of the separation cylinder is provided with a separation component for docking with the lower cylinder cover and separating the supernatant from the sediment; The separation component includes a sleeve and an annular rubber ring, the sleeve is sleeved on the cylinder body near the bottom of the separation cylinder and is elastically connected to the separation cylinder, and an inner support frame is fixedly installed at the bottom of the sleeve, a drum-shaped blocking column is installed on the inner support frame, and the annular rubber ring is fixedly embedded on the inner cylinder wall of the separation cylinder and is located below the drum-shaped blocking column; The lysozyme extraction and separation equipment further comprises a driving component, through which the separation cylinder and the L-shaped stirring sheet are driven to rotate.

[0006] Preferably, a support rod coaxially arranged with the sleeve is fixedly mounted on the inner support frame, the drum-shaped blocking column is fixedly mounted on the top of the support rod, and the drum-shaped blocking column is interference fit with the annular rubber ring.

[0007] Preferably, a plurality of annular blanking cavities are provided on the inner support frame, and a threaded docking port for threadedly connecting the lower cylinder cover is fixedly embedded at the bottom of the inner support frame.

[0008] Preferably, a rotating disk is rotatably connected to the middle of the upper cylinder cover, and a lifting and rotating component is installed on the rotating disk for driving the L-shaped stirring blade to reciprocate and rise and fall and rotate at high speed in the separation cylinder; The lifting and rotating component includes a reciprocating screw, which is rotatably mounted on a rotating disk and coaxially arranged on the rotating disk, and the top of the reciprocating screw extends out of the rotating disk and is provided with a cross groove, and a limiting rod arranged parallel to the reciprocating screw is also fixedly mounted on the rotating disk, and the top of the limiting rod extends out of the rotating disk and is fixedly engaged with a threaded A column, the reciprocating screw is provided with a threaded seat and a threaded sleeve distributed up and down and threadedly connected, and the threaded seat and the threaded sleeve are rotatably connected, the limiting rod passes through a through hole opened in the threaded seat and is slidably connected to the through hole, and the L-shaped stirring sheet is fixedly mounted on the bottom of the threaded sleeve.

[0009] Preferably, the driving component includes an outer gear ring and an inner gear ring, both of which are rotatably mounted on the centrifugal table and coaxially arranged with the insertion tube through hole opened at the axis center of the centrifugal table, and the inner gear ring is located at the periphery of the outer gear ring, and a meshing middle gear is installed between the outer gear ring and the inner gear ring, and a servo motor for driving the middle gear to rotate is fixedly installed on the centrifugal table.

[0010] Preferably, a plurality of annularly distributed mounting ears are fixedly embedded on the body of the separation cylinder, a plurality of annularly distributed ear slots matching the mounting ears are provided on the outer gear ring, and a threaded hole is provided on each of the ear slots.

[0011] Preferably, a connecting rod component for connecting the reciprocating screw is installed on the inner gear ring; The connecting rod component includes a bracket column, which is rotatably mounted on the inner gear ring, and a micro cylinder is fixedly mounted on the bracket column, the output end of the micro cylinder is vertically upward and fixedly mounted with a horizontally arranged center connecting plate, and a cross plug plate is fixedly mounted on the center connecting plate, and the cross plug plate matches the cross slot opened on the top of the reciprocating screw.

[0012] Preferably, a positioning rod is fixedly installed on the centrifugal table between the outer gear ring and the inner gear ring, and a threaded B column is fixedly installed on the positioning rod, and a double-head sleeve plate fixed by a fastening nut is sleeved between the threaded B column and the threaded A column.

[0013] Preferably, the elastic members are provided in multiple groups and are distributed in an annular manner on the top of the sleeve, the elastic members include a sliding rod, the sliding rod is fixedly installed on the top of the sleeve and passes through the outer ring edge integrally formed on the outer wall of the separation cylinder, and the sliding rod is slidably connected to the outer ring edge, a spring in a compressed state is sleeved on the sliding rod, and the two ends of the spring are respectively fixedly connected to the lower surface of the outer ring edge and the upper surface of the sleeve, and a rod cap is fixedly installed on the top of the sliding rod.

[0014] Preferably, an elastic corrugated sleeve coaxially arranged with the separation cylinder is fixedly engaged at the bottom of the separation cylinder and the edge of the inner support frame, and a circle of arc-shaped outer warping edge is fixedly engaged at the cover opening of the lower cylinder cover.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention adopts a single motor working mode to drive the separation cylinder and the L-shaped stirring sheet to start, so that the separation cylinder can effectively mix the plant tissue fluid and the buffer solution before centrifugation. Since the L-shaped stirring sheet can be reciprocated, the crushing effect of the tissue fragments in the mixture is improved, so that the tissue fragments release lysozyme more fully, and more lysozyme is collected; 2. After the centrifugal work is completed, the separation component can be used to peel the sediment into the lower cylinder cover, and then the lower cylinder cover is screwed off from the threaded docking interface to quickly separate and collect the sediment from the separation cylinder without affecting the supernatant that has been standing still on the upper part of the separation cylinder. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 A schematic structural diagram of a preferred embodiment of the lysozyme extraction and separation device provided by the present invention; Figure 2 for Figure 1 The schematic diagram of the structure after the separation cylinder is separated from the upper cylinder cover and the lower cylinder cover; Figure 3 for Figure 2 A schematic diagram of the cross-sectional structure when the upper cylinder cover and the lower cylinder cover are installed on the separation cylinder; Figure 4 for Figure 3 A schematic cross-sectional view of the upper cylinder cover; Figure 5 for Figure 3 A schematic cross-sectional structural diagram of the separation cylinder; Figure 6 for Figure 3 A schematic cross-sectional view of the lower cylinder cover; Figure 7 for Figure 1 A schematic diagram of the structure of the centrifugal table when no separation cylinder is installed; Figure 8 for Figure 7 A schematic diagram of the structure of the bottom of the centrifugal table when the centrifugal table is not equipped with a separation cylinder; Fig. 9 for Figure 7 A schematic diagram of the structure of the connecting rod component; Fig.10 for Figure 1 A schematic structural diagram of the double-head sleeve plate; Fig.11 for Figure 4 A schematic diagram of the enlarged structure of the junction at A.

[0017] In the figure: 1, centrifugal table; 1a, inserting hole; 2, separation cylinder; 21, upper cylinder cover; 211, rotating disk; 22, lower cylinder cover; 221, arc-shaped outer edge; 23, mounting ear piece; 24, separation component; 241, sleeve tube; 242, threaded interface; 243, inner support frame; 243a, blanking cavity; 244, support rod; 245, drum-shaped blocking column; 246, annular rubber ring; 25, outer ring edge; 3, L-shaped stirring sheet; 4, driving component; 41, outer gear ring; 41a, ear piece groove; 41b , threaded hole; 42, inner gear ring; 43, center gear; 44, servo motor; 5, lifting and rotating component; 51, reciprocating screw; 51a, cross groove; 52, limit rod; 521, threaded A column; 53, threaded seat; 54, threaded sleeve; 6, connecting rod component; 61, bracket column; 62, micro cylinder; 63, center plate; 64, cross plug plate; 7, positioning rod; 71, threaded B column; 72, double-head sleeve plate; 73, fastening nut; 8, elastic part; 81, sliding rod; 82, spring; 9, elastic corrugated sleeve. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figures 1 to 11 An embodiment of the present invention provides a lysozyme extraction and separation device, which includes a centrifuge table 1, a separation cylinder 2, an L-shaped stirring sheet 3 and a driving component 4.

[0020] In the embodiments of the present invention, please refer to Figure 1 , Figure 2 , Figure 7 as well as Figure 8 A separation cylinder 2 for centrifuging the extract is installed on the centrifugal table 1, and both upper and lower end surfaces of the separation cylinder 2 are open structures, and upper and lower ends of the separation cylinder 2 are respectively installed with an upper cylinder cover 21 and a lower cylinder cover 22 which are threadedly connected and used to seal the end surface openings; The lysozyme extraction and separation equipment also includes a driving component 4, which includes an outer gear ring 41 and an inner gear ring 42. The outer gear ring 41 and the inner gear ring 42 are both rotatably mounted on the centrifugal table 1 and are coaxially arranged with the insertion hole 1a opened at the axis of the centrifugal table 1, and the inner gear ring 42 is located at the outer periphery of the outer gear ring 41. A meshing gear 43 is installed between the outer gear ring 41 and the inner gear ring 42. A servo motor 44 for driving the rotation of the middle gear 43 is fixedly installed on the centrifugal table 1, and the insertion hole 1a is opened at the axis of the centrifugal table 1. A plurality of annularly distributed mounting ears 23 are fixedly embedded on the body of the separation cylinder 2, and a plurality of annularly distributed ear slots 41a are opened on the outer gear ring 41 and matched with the mounting ears 23, and each ear slot 41a is opened with a threaded hole 41b.

[0021] It should be noted that: the lower cylinder cover 22 is threadedly connected to the threaded docking port 242 on the separation cylinder 2, thereby blocking the opening at the bottom of the separation cylinder 2, and then the extract is poured into the separation cylinder 2 from the top opening of the separation cylinder 2, and then the cylinder cover 21 is closed with the threaded cover, and the separation cylinder 2 is inserted into the insertion hole 1a opened in the centrifugal table 1 until the mounting ear 23 falls into the ear groove 41a, and then the mounting ear 23 is fixed to the threaded hole 41b opened in the outer gear ring 41 by screws, thereby completing the installation of the separation cylinder 2 on the outer gear ring 41, and subsequently turning on the servo motor 44 can drive the inner gear ring 42 to rotate through the intermediate gear 43, so that the separation cylinder 2 can rotate at high speed and drive the internal extract to separate the supernatant and the precipitate.

[0022] In order to reduce the force of the separation cylinder 2 passing through the insertion tube through hole 1a and colliding with the hole wall thereof, a rubber ring is sleeved on the insertion tube through hole 1a, thereby effectively protecting the separation cylinder 2.

[0023] In order to better observe the separation effect of the extract in the separation cylinder 2, the separation cylinder 2 can be made of high-strength transparent glass.

[0024] In the embodiments of the present invention, please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 7 , Fig. 9 , Fig.10 as well as Fig.11 The upper cylinder cover 21 is provided with a plurality of L-shaped stirring slices 3 distributed in an annular manner, and the extract is stirred and crushed by the L-shaped stirring slices 3. Specifically, a rotating disk 211 connected in rotation is provided in the middle of the upper cylinder cover 21, and a lifting and rotating component 5 for driving the L-shaped stirring slices 3 to reciprocate and rise and fall and rotate at high speed in the separation cylinder 2 is provided on the rotating disk 211. The lifting and rotating component 5 includes a reciprocating screw 51, which is rotatably installed on the rotating disk 211 and is coaxially arranged with the rotating disk 211, and a rotating shaft 51 extends from the top of the reciprocating screw 51. The disk 211 is provided with a cross groove 51a, and a limiting rod 52 arranged parallel to the reciprocating screw 51 is fixedly mounted on the rotating disk 211, and the top of the limiting rod 52 extends out of the rotating disk 211 and is fixedly engaged with a threaded A column 521, and a threaded seat 53 and a threaded sleeve 54 which are distributed up and down and threadedly connected are sleeved on the reciprocating screw 51, and the threaded seat 53 and the threaded sleeve 54 are rotatably connected, and the limiting rod 52 passes through the through hole provided in the threaded seat 53 and is slidably connected with the through hole, and the L-shaped stirring sheet 3 is fixedly mounted on the bottom of the threaded sleeve 54; When the L-shaped stirring blade 3 is needed to stir and crush the extract, a connecting rod component 6 and a double-headed sleeve plate 72 are added to enable the driving component 4 to drive the separation cylinder 2 to rotate in the opposite direction to the L-shaped stirring blade 3. Specifically, a connecting rod component 6 for connecting the reciprocating screw 51 is installed on the outer gear ring 41, and the connecting rod component 6 includes a bracket column 61, which is rotatably installed on the inner gear ring 42, and a micro cylinder 62 is fixedly installed on the bracket column 61, and the output end of the micro cylinder 62 is vertically upward and fixedly installed with a horizontally arranged middle connecting plate 63, and a cross plug plate 64 is fixedly installed on the middle connecting plate 63, and the cross plug plate 64 is matched with the cross groove 51a opened at the top of the reciprocating screw 51, and a positioning rod 7 is fixedly installed on the centrifugal table 1 between the outer gear ring 41 and the inner gear ring 42, and a threaded B column 71 is fixedly installed on the positioning rod 7, and a double-headed sleeve plate 72 fixed by a fastening nut 73 is sleeved between the threaded B column 71 and the threaded A column 521.

[0025] The locking nut 73 is then installed on the locking nut 71 of the locking nut 73 to lock the locking nut 73. The locking nut 73 is then installed on the locking nut 73 to lock the locking nut 73.

[0026] Since the intermediate gear 43 is located between the outer gear ring 41 and the inner gear ring 42 and meshes with the outer gear ring 41 and the inner gear ring 42 respectively, when the servo motor 44 is started, the separation cylinder 2 rotates in the opposite direction to the reciprocating screw 51. Since the threaded seat 53 and the threaded sleeve 54 are rotatably connected and are both threadedly connected to the reciprocating screw 51, and the threaded seat 53 is limited by the limit rod 52, the threaded seat 53 can be lifted and lowered along the reciprocating screw 51, and the threaded sleeve 54 is driven to rotate while sliding back and forth along the reciprocating screw 51 under the action of the downward thrust or upward pulling force of the threaded seat 53, so that the L-shaped stirring sheet 3 at the bottom of the threaded sleeve 54 can stir and crush the extract in the separation cylinder 2; The rotating L-shaped stirring blade 3 can enhance the mixing of the plant tissue fluid and the buffer solution. Since the L-shaped stirring blade 3 on the threaded sleeve 54 can be reciprocated and lifted, the stirring center can be continuously changed, thereby further achieving the degree of mixing of the plant tissue fluid and the buffer solution. In addition, the L-shaped stirring blade 3 can perform secondary crushing of tissue fragments in the plant tissue fluid, thereby allowing the lysozyme to be released more fully.

[0027] It should also be noted that: after stirring for a period of time, the micro-cylinder 62 is turned on to cause the cross-plate 64 to slip out of the cross-slot 51a formed by the reciprocating screw 51, so that the reciprocating screw 51 loses its rotational driving force, causing the reciprocating screw 51 and the L-shaped stirring blade 3 to gradually stop. Therefore, the stirring effect of the L-shaped stirring blade 3 is cancelled and the separation cylinder 2 continues to rotate at a high speed, thereby avoiding the stirring of the mixed liquid by the L-shaped stirring blade 3, which makes it difficult to effectively separate the supernatant and the precipitate in the mixed liquid.

[0028] This solution adopts a single-motor working mode to drive the separation cylinder 2 and the L-shaped stirring blade 3 to start, so that the separation cylinder 2 can effectively mix the plant tissue fluid and the buffer solution before centrifuging them. Since the L-shaped stirring blade 3 can be reciprocated and lifted, the crushing effect of the tissue fragments in the mixture is greatly improved, so that the tissue fragments release lysozyme more fully, and more lysozyme is collected. When the stirring and crushing are completed, the stirring and crushing action of the L-shaped stirring blade 3 can be stopped without stopping, and the separation cylinder 2 can still work normally by centrifugation.

[0029] Among them, blade surfaces are arranged on both sides of the horizontal part of the L-shaped stirring blade 3, so that tissue fragments can be better crushed, and the vertical part of the L-shaped stirring blade 3 is higher than the length of the limiting rod 52 to prevent the limiting rod 52 from hindering the rotation of the horizontal part of the L-shaped stirring blade 3.

[0030] It is worth noting that: before installing the separation cylinder 2 on the outer gear ring 41, the bracket column 61 is rotated to make the center plate 63 deviate from the outer gear ring 41. When the separation cylinder 2 is installed, the bracket column 61 is turned back to make the center line of the cross plug plate 64 on the center plate 63 and the axis of the outer gear ring 41 colinear, and the bracket column 61 is rotated and set on the inner gear ring 42, so that the center plate 63 does not hinder the installation and removal of the separation cylinder 2.

[0031] In this solution, in order to avoid mutual interference between the double-head cover plate 72 and the middle connecting plate 63 , the height of the double-head cover plate 72 is installed lower than the height of the middle connecting plate 63 .

[0032] In the embodiments of the present invention, please refer to Figure 1 , Figure 2 , Figure 3 , Figure 5 as well as Figure 6 , a separation component 24 for docking with the lower cylinder cover 22 and separating the supernatant and the sediment is installed at the bottom of the separation cylinder 2, and the separation component 24 includes a sleeve 241 and an annular rubber ring 246, the sleeve 241 is sleeved on the cylinder body of the separation cylinder 2 near the bottom and is elastically connected to the separation cylinder 2, specifically, an elastic member 8 is arranged between the separation cylinder 2 and the sleeve 241, and the elastic member 8 is provided with multiple groups and annularly distributed on the top of the sleeve 241, and the elastic member 8 includes a sliding rod 81, the sliding rod 81 is fixedly installed on the top of the sleeve 241 and penetrates the outer ring edge 25 integrally formed on the outer cylinder wall of the separation cylinder 2, and the sliding rod 81 is slidably connected to the outer ring edge 25, a spring 82 in a compressed state is sleeved on the sliding rod 81, and the two ends of the spring 82 are respectively fixedly connected to the lower surface of the outer ring edge 25 and the upper surface of the sleeve 241, and a rod cap is fixedly installed on the top of the sliding rod 81; An inner support frame 243 is fixedly installed at the bottom of the sleeve tube 241, and a drum-shaped sealing column 245 is installed on the inner support frame 243. The annular rubber ring 246 is fixedly embedded in the inner tube wall of the separation tube 2 and is located below the drum-shaped sealing column 245. Specifically, a support rod 244 coaxially arranged with the sleeve tube 241 is fixedly installed on the inner support frame 243, and the drum-shaped sealing column 245 is fixedly installed on the top of the support rod 244, and the drum-shaped sealing column 245 and the annular rubber ring 246 are interference fit, and a plurality of annular blanking cavities 243a are opened on the inner support frame 243, and a threaded docking port 242 for threaded connection to the lower tube cover 22 is fixedly embedded at the bottom of the inner support frame 243.

[0033] It should be noted that: due to the action of the spring 82, the sleeve pipe 241 drives the drum-shaped blocking column 245 in the separation component 24 to fit into the annular rubber ring 246 and form a sealing structure, thereby sealing the bottom of the separation cylinder 2. When the centrifugation is completed, the separation cylinder 2 is removed from the centrifuge table 1. At this time, the sediment is located on the conical column above the drum-shaped blocking column 245. The sleeve pipe 241 is pushed up to make the sleeve pipe 241 slide up along the separation cylinder 2 (the spring 82 is further compressed during this process), so that the drum-shaped blocking column 245 slides out of the annular rubber ring 246 and opens the formed sealing structure. Therefore, the sediment on the conical column above the drum-shaped blocking column 245 can slide down along the conical column above the drum-shaped blocking column 245, and fall into the lower cylinder cover 22 through the blanking cavity 243a opened on the inner support frame 243. After the sediment is collected, the sleeve 241 is loosened, so that the sleeve 241, under the action of the elastic force of the spring 82, drives the drum-shaped sealing column 245 to fit into the annular rubber ring 246 and form a sealing structure. Then, the lower cylinder cover 22 is unscrewed from the threaded docking port 242, and the sediment can be quickly separated from the separation cylinder 2 and collected without affecting the supernatant that has been set still on the top of the separation cylinder 2.

[0034] It is worth noting that an outward-expanding opening is provided at the top of the annular rubber ring 246 , and the outward-expanding opening matches the conical column below the drum-shaped blocking column 245 , thereby improving the sealing performance of the sealing structure formed by the drum-shaped blocking column 245 and the annular rubber ring 246 .

[0035] Among them, an elastic corrugated sleeve 9 coaxially arranged with the separation cylinder 2 is fixedly embedded at the bottom of the separation cylinder 2 and the edge of the inner support frame 243 to prevent the solution from leaking from the bottom of the separation cylinder 2 and the edge of the inner support frame 243. The cover of the lower cylinder cover 22 is fixedly embedded with a circle of arc-shaped outer edge 221 to facilitate the discharge of sediment.

[0036] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A lysozyme extraction and separation device, comprising: a centrifuge table (1), and A separation cylinder (2) is mounted on the centrifugal table (1) and is used to centrifuge the extract through the centrifugal table (1), wherein both upper and lower end surfaces of the separation cylinder (2) are open structures, and upper and lower ends of the separation cylinder (2) are respectively provided with an upper cylinder cover (21) and a lower cylinder cover (22) which are threadedly connected and used to seal the end surface openings, and are characterized in that: The upper cylinder cover (21) is provided with a plurality of L-shaped stirring sheets (3) distributed in an annular manner, and the extract is stirred and crushed by the L-shaped stirring sheets (3); the bottom of the separation cylinder (2) is provided with a separation component (24) for docking with the lower cylinder cover (22) and separating the supernatant from the sediment; The separation component (24) comprises a sleeve tube (241) and an annular rubber ring (246); the sleeve tube (241) is sleeved on a cylinder body of the separation cylinder (2) near the bottom and is elastically connected to the separation cylinder (2); an inner support frame (243) is fixedly mounted on the bottom of the sleeve tube (241); a drum-shaped blocking column (245) is mounted on the inner support frame (243); and the annular rubber ring (246) is fixedly engaged with the inner cylinder wall of the separation cylinder (2) and is located below the drum-shaped blocking column (245); The lysozyme extraction and separation device further comprises a driving component (4), through which the separation cylinder (2) and the L-shaped stirring sheet (3) are driven to rotate.

2. The lysozyme extraction and separation device according to claim 1, characterized in that: A support rod (244) coaxially arranged with the sleeve tube (241) is fixedly mounted on the inner support frame (243), and the drum-shaped blocking column (245) is fixedly mounted on the top of the support rod (244), and the drum-shaped blocking column (245) is interference-fitted with the annular rubber ring (246).

3. The lysozyme extraction and separation device according to claim 2, characterized in that: The inner support frame (243) is provided with a plurality of annularly distributed blanking cavities (243a), and a threaded docking port (242) for threaded connection with the lower cylinder cover (22) is fixedly engaged at the bottom of the inner support frame (243).

4. The lysozyme extraction and separation device according to claim 1, characterized in that: A rotatably connected rotating disk (211) is mounted in the middle of the upper cylinder cover (21), and a lifting and rotating component (5) is mounted on the rotating disk (211) for driving the L-shaped stirring blade (3) to reciprocate and rise and fall and rotate at high speed in the separation cylinder (2); The lifting and rotating component (5) comprises a reciprocating screw (51), the reciprocating screw (51) being rotatably mounted on a rotating disk (211) and being coaxially arranged with the rotating disk (211), the top of the reciprocating screw (51) extending out of the rotating disk (211) and being provided with a cross groove (51a), a limiting rod (52) being fixedly mounted on the rotating disk (211) and being arranged parallel to the reciprocating screw (51), the top of the limiting rod (52) extending out of the rotating disk (211) and being fixedly engaged with a threaded A column (521), a threaded seat (53) and a threaded sleeve (54) being arranged in upper and lower distributions and being threadedly connected, the threaded seat (53) and the threaded sleeve (54) being rotatably connected, the limiting rod (52) passing through a through hole provided in the threaded seat (53) and being slidably connected with the through hole, and the L-shaped stirring sheet (3) being fixedly mounted on the bottom of the threaded sleeve (54).

5. The lysozyme extraction and separation device according to claim 4, characterized in that: The driving component (4) comprises an outer gear ring (41) and an inner gear ring (42), the outer gear ring (41) and the inner gear ring (42) are both rotatably mounted on the centrifugal table (1) and are coaxially arranged with a tube through hole (1a) opened at the axis of the centrifugal table (1), and the inner gear ring (42) is located at the periphery of the outer gear ring (41), a meshing gear (43) is mounted between the outer gear ring (41) and the inner gear ring (42), and a servo motor (44) for driving the rotation of the middle gear (43) is fixedly mounted on the centrifugal table (1).

6. The lysozyme extraction and separation device according to claim 5, characterized in that: The body of the separation cylinder (2) is fixedly fitted with a plurality of annularly distributed mounting ears (23), the outer gear ring (41) is provided with a plurality of annularly distributed ear slots (41a) matching the mounting ears (23), and each of the ear slots (41a) is provided with a threaded hole (41b).

7. The lysozyme extraction and separation device according to claim 5, characterized in that: A connecting rod component (6) for connecting to a reciprocating screw (51) is mounted on the inner gear ring (42); The link rod component (6) comprises a support column (61), the support column (61) being rotatably mounted on the inner gear ring (42), and a micro cylinder (62) being fixedly mounted on the support column (61), an output end of the micro cylinder (62) being vertically upward and fixedly mounted with a horizontally arranged middle link plate (63), and a cross plug plate (64) being fixedly mounted on the middle link plate (63), and the cross plug plate (64) being matched with a cross slot (51a) provided on the top of the reciprocating screw (51).

8. The lysozyme extraction and separation device according to claim 7, characterized in that: A positioning rod (7) is fixedly mounted on the centrifugal table (1) between the outer gear ring (41) and the inner gear ring (42), and a threaded B column (71) is fixedly mounted on the positioning rod (7), and a double-head sleeve plate (72) fixed by a fastening nut (73) is sleeved between the threaded B column (71) and the threaded A column (521).

9. The lysozyme extraction and separation device according to claim 1, characterized in that: An elastic member (8) is provided between the separation cylinder (2) and the sleeve tube (241). The elastic member (8) is provided in multiple groups and is distributed in an annular manner on the top of the sleeve tube (241). The elastic member (8) comprises a sliding rod (81). The sliding rod (81) is fixedly mounted on the top of the sleeve tube (241) and penetrates an outer ring edge (25) integrally formed on the outer cylinder wall of the separation cylinder (2). The sliding rod (81) is slidably connected to the outer ring edge (25). A spring (82) in a compressed state is sleeved on the sliding rod (81), and two ends of the spring (82) are respectively fixedly connected to the lower surface of the outer ring edge (25) and the upper surface of the sleeve tube (241). A rod cap is fixedly mounted on the top of the sliding rod (81).

10. The lysozyme extraction and separation device according to claim 1, characterized in that: An elastic corrugated sleeve (9) coaxially arranged with the separation cylinder (2) is fixedly engaged at the bottom of the separation cylinder (2) and the edge of the inner support frame (243), and a circle of arc-shaped outer warping edge (221) is fixedly engaged at the cover opening of the lower cylinder cover (22).

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