Protein crystallization device with high environmental stability

Abstract

The invention discloses a protein crystallization device with high environmental stability, and relates to the technical field of bioengineering, the protein crystallization device comprises a workbench, a positioning assembly is assembled on the top of the workbench, and a liquid dropping assembly for adding a crystallization solution is assembled on one side of the positioning assembly and located on the top of the workbench; the liquid dropping assembly comprises a first driving frame transversely fixed to the top of the workbench, a second driving frame is longitudinally assembled in the first driving frame, a third driving frame is transversely installed in the second driving frame, a liquid dropping barrel is arranged on the front surface of the third driving frame, and a dropping nozzle is arranged at the bottom end of the liquid dropping barrel. The purpose of efficiently dripping a crystallization solution on a crystallization plate is achieved through the three sets of driving frames, the deviation condition existing in manual operation is eliminated, it is ensured that the quality of the process from protein to crystallization is high, the internal crystallization solution can be squeezed out when the piston ascends and descends in the solution cylinder, and the crystallization solution can be more efficiently dripped on the crystallization plate.

Description

Technical Field

[0001] The present invention relates to the technical field of bioengineering, and particularly to a protein crystallization device with high environmental stability. Background Technique

[0002] With the continuous development of modern society and the continuous improvement of economic level, the exploration of scientific research in modern society is also increasing. In the existing science and technology, biological science, as a technology for studying life, cells, DNA, proteins and other life-bearing substances, is also gradually developing. In the technology of biological science, proteins, as the embodiers of life activities, their structures determine their functions. In the process of existing proteins, it is often necessary to crystallize proteins so that proteins can be analyzed by rays. Based on this, in order to crystallize proteins, a protein crystallization device is needed to crystallize saturated protein solutions.

[0003] At present, the method for sealing a 24-well crystallization plate is manual greasing and sealing. The proficiency of manual operation and the application of vacuum sealing grease by feeling will affect the sealing effect of the 24-well crystallization plate. A 24-well crystallization plate with a poor sealing effect cannot be used for protein crystallization, resulting in the scrapping of the 24-well crystallization plate and affecting the cost. Therefore, a protein crystallization device with high environmental stability is proposed. Summary of the Invention

[0004] Based on this, the purpose of the present invention is to provide a protein crystallization device with high environmental stability to solve the technical problems raised in the above background.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A protein crystallization device with high environmental stability, including a workbench, a positioning component is assembled on the top of the workbench, and a dropping component for adding crystallization solution is assembled on the top of the workbench on one side of the positioning component;

[0006] The dropping component includes a first driving frame horizontally fixed on the top of the workbench. A second driving frame is longitudinally assembled in the first driving frame. A third driving frame is horizontally installed in the second driving frame. A dropping cylinder is provided on the front surface of the third driving frame. A dropping nozzle is provided at the bottom end of the dropping cylinder. An electric push rod is installed on the top of the dropping cylinder. A piston is movably arranged in the electric push rod. A connecting rod connected to the telescopic end of the electric push rod is fixed on the top of the piston. A liquid inlet pipe is connected to the outer wall of the dropping cylinder near the bottom position on one side. A branch pipe connected to the outer wall of the dropping cylinder near the top position is provided on the outer wall of the liquid inlet pipe. An output pipe is connected to the outer wall of the dropping cylinder near the top position on the other side. The end of the output pipe is connected to the dropping nozzle.

[0007] As a preferred technical solution, a storage rack is provided on the top of the workbench on one side of the positioning component.

[0008] As a preferred technical solution, the positioning assembly includes a disk frame rotatably provided on the top of the workbench. A plurality of groups of notches are formed on the top of the disk frame. A crystallization plate is assembled in each group of notches. A support plate is movably provided in the disk frame at the bottom of the crystallization plate. The bottom end of the support plate is sleeved with and fixed to a U-shaped seat on the inner wall of the upper part of the disk frame. Constraint cylinders are arranged on both sides of each crystallization plate in the disk frame. Ball head rods extending into the constraint cylinders are arranged at both ends of the support plate. An annular groove is reserved at the contact position between the curved surface of the constraint cylinder and the end of the ball head rod. A stepping motor for driving the disk frame to rotate is assembled at the bottom of the workbench. The end of the liquid inlet pipe extends into the liquid storage frame through a strip groove.

[0009] As a preferred technical solution, the lower inner wall of the annular groove is convex near the front surface of the workbench.

[0010] As a preferred technical solution, a strip groove is formed on the top of the workbench between the disk frame and the first driving frame. A liquid storage frame is fixed at the corresponding position of the bottom of the workbench and the strip groove.

[0011] As a preferred technical solution, check valves are assembled at one ends of the liquid inlet pipe, the branch pipe, the drip nozzle and the output pipe close to the drip cylinder.

[0012] As a preferred technical solution, the first driving frame includes a hollow frame body. A lead screw is rotatably provided in the frame body. A motor for driving the lead screw to rotate is installed at the end of the frame body. The liquid inlet pipe and the third driving frame have the same structure as the first driving frame.

[0013] In summary, the present invention mainly has the following beneficial effects:

[0014] The present invention realizes the purpose of efficiently dripping crystallization solution on the crystallization plate through three groups of driving frames, eliminates the deviation condition in manual operation, ensures high quality of the process of protein crystallization, and the piston can squeeze out the internal crystallization solution when lifting or lowering in the solution cylinder, realizing more efficient dripping of crystallization solution on the crystallization plate.

[0015] The present invention uses a rotating disk frame to continuously drip crystallization solution on the crystallization plate, improves the overall crystallization efficiency of proteins. At the same time, when rotating the disk frame, the crystallization plate is hidden in the notch, which has a constraining effect on the crystallization plate, preventing the crystallization plate from shifting due to centrifugal force. And the crystallization plate that has been dripped with crystallization solution can automatically lift up after rotating one week, thus facilitating manual removal of the crystallization plate, achieving the purpose of providing convenience for manual operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a three-dimensional structure diagram of the present invention;

[0017] Figure 2 is a structure diagram of the liquid dripping assembly of the present invention;

[0018] Figure 3 It is the bottom view of the workbench of the present invention;

[0019] Figure 4 It is the partially cut-away view of the workbench of the present invention;

[0020] Figure 5 It is the cut-away plan view of the dropping cylinder of the present invention;

[0021] Figure 6 It is the bottom view structure diagram of the disk frame of the present invention;

[0022] Figure 7 It is the front view plan view of the inner cylinder of the present invention.

[0023] In the figure: 100, workbench; 200, positioning component; 300, dropping component;

[0024] 110, liquid storage frame; 120, strip groove;

[0025] 210, stepping motor; 220, disk frame; 230, crystallization plate; 240, notch; 250, restraint cylinder; 251, annular groove; 260, U-shaped seat; 270, support plate; 280, ball head rod;

[0026] 310, first driving frame; 320, second driving frame; 330, third driving frame; 340, positioning seat; 350, electric push rod; 360, dropping cylinder; 370, liquid inlet pipe; 371, branch pipe; 380, output pipe; 390, piston; 391, connecting rod. Detailed implementation manners

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present invention and should not be construed as a limitation to the present invention.

[0028] Next, the embodiments of the present invention will be described according to the overall structure of the present invention.

[0029] A protein crystallization device with high environmental stability, as Figures 1 to 7 shown, includes a workbench 100. A positioning component 200 is assembled on the top of the workbench 100, and a dropping component 300 for adding crystallization solution is assembled on one side of the positioning component 200 on the top of the workbench 100;

[0030] The dropping liquid assembly 300 includes a first driving frame 310 horizontally fixed to the top of the workbench 100. A second driving frame 320 is longitudinally assembled in the first driving frame 310. A third driving frame 330 is horizontally installed in the second driving frame 320. A dropping liquid cylinder 360 is provided on the front surface of the third driving frame 330. A dropping nozzle is provided at the bottom end of the dropping liquid cylinder 360. An electric push rod 350 is installed at the top of the dropping liquid cylinder 360. A piston 390 is movably provided in the electric push rod 350. A connecting rod 391 fixed to the top of the piston 390 and connected to the telescopic end of the electric push rod 350. A liquid inlet pipe 370 is connected to the outer wall of one side of the dropping liquid cylinder 360 near the bottom. A branch pipe 371 whose outer wall is connected to the outer wall of the dropping liquid cylinder 360 near the top is provided on the outer wall of the liquid inlet pipe 370. An output pipe 380 is connected to the outer wall of the other side of the dropping liquid cylinder 360 and near the top. The end of the output pipe 380 is connected to the dropping nozzle;

[0031] The positioning assembly 200 includes a disk frame 220 rotatably provided on the top of the workbench 100. A plurality of groups of notches 240 are formed on the top of the disk frame 220. A crystallization plate 230 is assembled in each group of notches 240. A support plate 270 is movably provided inside the disk frame 220 at the bottom of the crystallization plate 230. A U-shaped seat 260 sleeved at the bottom end of the support plate 270 and fixed to the inner upper wall of the disk frame 220. Constraint cylinders 250 are provided on both sides of each crystallization plate 230 inside the disk frame 220. Ball head rods 280 extending into the constraint cylinders 250 are provided at both ends of the support plate 270. An annular groove 251 is reserved at the contact position between the curved surface of the constraint cylinder 250 and the end of the ball head rod 280. A stepping motor 210 for driving the disk frame 220 to rotate is assembled at the bottom of the workbench 100. The end of the liquid inlet pipe 370 extends into the liquid storage frame 110 through the slot 120.

[0032] Place the crystallization plate 230 containing protein on the support plate 270 on the top of the disk frame 220. At this time, the stepping motor 210 will drive the disk frame 220 to rotate by ninety degrees, and the dropping liquid assembly 300 will continuously drop the crystallization solution onto the crystallization plate 230. The three driving frames move the dropping liquid cylinder 360 to directly above the groove on the crystallization plate 230. At this time, the telescopic end of the electric push rod 350 descends, causing the piston to rise and fall in the dropping liquid cylinder 360, squeezing out the internal crystallization solution and dropping it into the groove on the crystallization plate 230 for the purpose of protein crystallization. At this time, continue to place the crystallization plate 230 on the support plate 270, and the stepping motor 210 will continue to drive the disk frame 220 to rotate by ninety degrees. Finally, the crystallization plate 230 onto which the crystallization solution has been dropped rotates one week. Since the ball head rod 280 will rotate together with the disk frame 220, it will have a tendency to rise due to the convex structure in the annular groove 251, and rise in the U-shaped seat 260 through the support plate 270, making the top of the support plate 270 close to the top of the disk frame 220, so that the top of the crystallization plate 230 is higher than the disk frame 220, thus facilitating the artificial hand or using tools to take out the crystallization plate 230, which further improves the efficiency of protein crystallization.

[0033] Please refer particularly to Figure 1 , a storage rack is provided on one side of the top of the workbench 100 and is located at the positioning assembly 200.

[0034] It can store the crystallization plate with white paper to be stored, making it convenient for manual placement of the crystallization plate on the disk frame 220.

[0035] Please refer particularly to Figure 7 , the lower inner wall of the annular groove 251 near the front surface of the workbench 100 is convex.

[0036] The convex part lifts the crystallization plate that rotates one week on the top of the disk frame 220 upward, thus facilitating the manual removal of the crystallization plate that has dripped the crystallization solution.

[0037] Please refer particularly to Figure 1 , a slot 120 is opened between the disk frame 220 and the first driving frame 310 on the top of the workbench 100, and a liquid storage frame 110 is fixed at the position corresponding to the slot 120 at the bottom of the workbench 100.

[0038] So that the device can store enough crystallization solution and cooperate with the liquid dropping assembly 300 to efficiently carry out the crystallization process of proteins.

[0039] Please refer particularly to Figure 5 , one-way valves are assembled in the ends of the liquid inlet pipe 370, the branch pipe 371, the dropper and the output pipe 380 close to the liquid dropping cylinder 360.

[0040] When the piston 390 descends in the liquid dropping cylinder 360, the one-way valves at the ends of the dropper, the liquid inlet pipe 370 and the branch pipe 371 are in an open state, while the one-way valve at the end of the output pipe 380 is in a closed state. At this time, the crystallization solution below the piston 390 can be discharged through the dropper, and at the same time, the crystallization solution in the liquid inlet pipe 370 is conveyed to the top of the piston 390 through the branch pipe 371. On the contrary, the one-way valves at the ends of the dropper and the branch pipe 371 are in a closed state, the one-way valves at the ends of the output pipe 380 and the liquid inlet pipe 370 are in an open state, the crystallization solution on the top of the piston 390 is conveyed to the dropper through the output pipe 380 and discharged, and the crystallization solution is also conveyed to the bottom of the piston 390 through the liquid inlet pipe 370, so that the piston 390 can discharge the crystallization solution when ascending and descending, making the crystallization efficiency of proteins relatively high.

[0041] Please refer particularly to Figure 2, the first driving frame 310 includes a hollow frame body. A lead screw is rotatably provided inside the frame body. A motor for driving the rotation of the lead screw is installed at the end of the frame body. The liquid inlet pipe 370 and the third driving frame 330 both have the same structure as the first driving frame 310. One end of the second driving frame 320 is threadedly sleeved on the outer wall of the lead screw inside the first driving frame 310. The end of the third driving frame 330 is threadedly sleeved on the outer wall of the lead screw inside the second driving frame 320. A positioning seat 340 for fixing the electric push rod 350 and the dropping cylinder 360 is threadedly sleeved on the outer wall of the lead screw inside the third driving frame 330.

[0042] The first driving frame 310, the second driving frame 320 and the third driving frame 330 cooperate with each other to make the dropping cylinder 360 move in three-dimensional space, so as to realize the high-efficiency dropping of the crystallization solution of the protein.

[0043] During use, place the crystallization plate 230 containing the protein on the tray 270 at the top of the tray frame 220. At this time, the stepping motor 210 will drive the tray frame 220 to rotate by 90 degrees, and the dropping liquid assembly 300 will continuously drop the crystallization solution onto the crystallization plate 230. The three groups of driving frames make the dropping cylinder 360 move to directly above the groove on the crystallization plate 230. At this time, the telescopic end of the electric push rod 350 descends, causing the piston to rise and fall in the dropping cylinder 360, squeezing out the internal crystallization solution and dropping it into the groove on the crystallization plate 230 to achieve the purpose of protein crystallization. At this time, continue to place the crystallization plate 230 on the tray 270, and the stepping motor 210 will continue to drive the tray frame 220 to rotate by 90 degrees. Finally, the crystallization plate 230 on which the crystallization solution has been dropped rotates one week. Since the ball head rod 280 will rotate together with the tray frame 220, it will tend to rise due to the convex structure in the annular groove 251, and rise in the U seat 260 through the tray 270, so that the top of the tray 270 is close to the top of the tray frame 220, making the top of the crystallization plate 230 higher than the tray frame 220, thus facilitating the removal of the crystallization plate 230 by manual hands or tools. In this way, the efficiency of protein crystallization is further improved. The parts not involved in this device are the same as the prior art or can be implemented by using the prior art.

[0044] Although the embodiments of the present invention have been shown and described, the specific embodiments are only explanations of the present invention and are not limitations of the invention. The specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art can make modifications, substitutions and variations that do not contribute creatively to the embodiments according to needs, but as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A protein crystallization device with high environmental stability, comprising a workbench (100), characterized in that: The top of the workbench (100) is equipped with a positioning component (200), and one side of the positioning component (200) is located on the top of the workbench (100) and is equipped with a dripping component (300) for adding a crystallization solution; The liquid dripping assembly (300) comprises a first driving frame (310) which is transversely fixed to the top of the workbench (100); a second driving frame (320) is longitudinally mounted in the first driving frame (310); a third driving frame (330) is transversely mounted in the second driving frame (320); a liquid dripping tube (360) is disposed on the front surface of the third driving frame (330); a drip nozzle is disposed at the bottom end of the liquid dripping tube (360); an electric push rod (350) is mounted on the top of the liquid dripping tube (360); an electric push rod (350) is movable in the electric push rod (350); A piston (390) is provided, and a connecting rod (391) connected to the telescopic end of the electric push rod (350) is fixed on the top of the piston (390); a liquid inlet pipe (370) is connected to the outer wall of one side of the dripping tube (360) near the bottom; the outer wall of the liquid inlet pipe (370) is provided with a branch pipe (371) connected to the outer wall of the dripping tube (360) near the top; an output pipe (380) is connected to the outer wall of the other side of the dripping tube (360) near the top, and the end of the output pipe (380) is connected to the drip nozzle.

2. A protein crystallization device with high environmental stability according to claim 1, characterized in that: A storage rack is provided on the top of the workbench (100) and located on one side of the positioning assembly (200).

3. A protein crystallization device with high environmental stability according to claim 1, characterized in that: The positioning assembly (200) comprises a disk frame (220) rotatably arranged on the top of the workbench (100), the top of the disk frame (220) is provided with a plurality of groups of notches (240), each group of the notches (240) is provided with a crystallization plate (230), the bottom of the crystallization plate (230) is located in the disk frame (220) and a support plate (270) is movably arranged therein, the bottom end of the support plate (270) is sleeved with a U-seat (260) fixed to the upper inner wall of the disk frame (220), and each group of notches (240) is located in the disk frame (220) and a U-seat (260) is arranged therein. Constraint cylinders (250) are provided on both sides of the crystallization plate (230), and ball head rods (280) extending into the constraint cylinder (250) are provided at both ends of the support plate (270). An annular groove (251) is reserved at the contact position between the curved surface of the constraint cylinder (250) and the end of the ball head rod (280). A stepping motor (210) for driving the disk frame (220) to rotate is installed at the bottom of the workbench (100), and the end of the liquid inlet pipe (370) extends into the liquid storage frame (110) through the groove (120).

4. A protein crystallization device with high environmental stability according to claim 3, characterized in that: The lower inner wall of the annular groove (251) is convex near the front surface of the workbench (100).

5. The protein crystallization device with high environmental stability according to claim 1, characterized in that: A groove (120) is provided on the top of the workbench (100) between the disk frame (220) and the first driving frame (310), and a liquid storage frame (110) is fixed at a position corresponding to the groove (120) on the bottom of the workbench (100).

6. The protein crystallization device with high environmental stability according to claim 1, characterized in that: One-way valves are installed in the liquid inlet pipe (370), the branch pipe (371), the drip nozzle and the output pipe (380) at one end close to the dripping tube (360).

7. The protein crystallization device with high environmental stability according to claim 1, characterized in that: The first driving frame (310) comprises a hollow frame body, a screw rod is rotatably arranged in the frame body, and a motor for driving the screw rod to rotate is installed at the end of the frame body. The liquid inlet pipe (370) and the third driving frame (330) both have the same structure as the first driving frame (310).

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