A flexible exosome extractor and methods of use thereof

Abstract

The application discloses an operation-flexible exosome extractor and a use method thereof, relates to the technical field of exosome extractor structures, and comprises a main unit which comprises an extraction box and a hinge fixedly connected to one side of the extraction box. One side of the hinge is fixedly connected with a box door, and an axle seat is fixedly connected inside the extraction box. The centrifugal test tube is used to hold the product to be treated. When multi-stage centrifugation is performed, the sampling tube with different heights in the test tube is used to extract and treat the suspension in the corresponding layer. Meanwhile, the air injection tube and the piston are matched with each other to gradually increase the space air inside the centrifugal test tube, thereby increasing the pressure above the centrifugal test tube. The piston moves downward, thereby accumulating the suspension in each layer into the sampling tube, so that the separated liquid can be sampled and inspected in a certain degree to rapidly check the actual centrifugation effect, and then the sample is collected and treated through the sampling test tube.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of exosome extractor structure, and particularly relates to an exosome extractor with flexible operation and a use method thereof. BACKGROUND

[0002] Exosomes are disc-shaped vesicles with a diameter of 40-100 nanometers, and usually contain complex RNA, proteins, nucleic acids and lipids inside, so when cells release them, they can move between cells through the extracellular matrix, and then achieve the function of transmitting information through the combination of related substances. At the same time, exosomes are widely present in various body fluids in the human body, and are relatively easy to obtain, so for some diseases, exosomes have gradually begun to be extracted and analyzed to achieve the purpose of detection and diagnosis.

[0003] Generally, the extraction of exosomes is mainly realized by ultracentrifugation, but ultracentrifugation needs to be centrifuged several times to obtain the required substances, and after each centrifugation, the sampling may be dispersed due to touching and vibration, thereby affecting the centrifugation effect and increasing the extraction difficulty. At the same time, the extraction may be contaminated by external bacteria, and multiple centrifugations increase the risk, so that the extraction quality of exosomes may be reduced. SUMMARY

[0004] This section aims to summarize some aspects of the embodiments of the present application and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of the specification to avoid obscuring the purpose of this section, abstract and title, and such simplifications or omissions cannot be used to limit the scope of the present application.

[0005] In view of the above problems of the prior art, the present application is proposed.

[0006] Therefore, the present application aims to provide an exosome extractor with flexible operation and a use method thereof, which is suitable for solving the problem that multiple centrifugations are required to obtain the required substances, and after each centrifugation, the sampling may be dispersed due to touching and vibration, thereby affecting the centrifugation effect and increasing the extraction difficulty. At the same time, the extraction may be contaminated by external bacteria, and multiple centrifugations increase the risk, so that the extraction quality of exosomes may be reduced.

[0007] To solve the above technical problems, the present application provides the following technical solutions: an exosome extractor, and a use method of the exosome extractor, wherein the exosome extractor comprises:

[0008] The main unit comprises a extraction box, a hinge fixedly connected to one side of the extraction box, a box door fixedly connected to one side of the hinge, and a shaft seat fixedly connected to the inside of the extraction box;

[0009] The centrifugal sampling unit comprises a connecting plate hingedly connected to the inner wall of the extraction box, a rotating rod rotatably connected to the inner surface of the shaft seat, an arc-shaped structure on one side of the connecting plate, a load-bearing frame rotatably connected to one side of the arc-shaped structure, a first rubber wheel, a second rubber wheel, and a third rubber wheel equidistantly distributed on the outer surface of the rotating rod, and a plurality of groups of centrifuge test tubes circumferentially distributed in the load-bearing frame;

[0010] The adjustment driving unit comprises a support frame fixedly connected to one side of the extraction box, a special-shaped frame fixedly connected to one side of the support frame, a transmission wheel frictionally connected to one side of the first rubber wheel, a moving groove formed in the special-shaped frame, a rotating gear rotatably connected to the moving groove, a connecting block fixedly connected to the lower surface of the special-shaped frame, a connecting shaft fixedly connected to one side of the connecting block, and an auxiliary wheel rotatably connected to the outer surface of the connecting shaft.

[0011] As a preferred scheme of the exosome extractor, the upper surface of the load-bearing frame is insertedly connected with a connecting block, one end of the rotating rod is fixedly connected to the lower surface of the load-bearing frame, and an auxiliary groove is formed in the outer surface of the load-bearing frame, and the inner diameter of the auxiliary groove is adapted to the connecting plate.

[0012] As a preferred scheme of the exosome extractor, the lower surface of the load-bearing frame is fixedly connected with an auxiliary ring, a limiting ring is rotatably connected to the inner surface of the auxiliary ring, one side of the limiting ring away from the auxiliary ring is fixedly connected to the outer surface of the rotating rod, and one end of the centrifuge test tube is hingedly connected with a test tube cover, and a sampling tube and an air injection tube are respectively fixedly connected to the inside of the test tube cover.

[0013] As a preferred scheme of the exosome extractor, the number of the sampling tubes is several and they are symmetrically distributed, the tube openings of the sampling tubes inside the centrifuge test tube are equidistantly distributed, one end of the sampling tube is fixedly connected with a connecting tube, one end of the connecting tube is hingedly connected with a guide tube, and one end of the guide tube is insertedly connected with a rubber pad.

[0014] As a preferred scheme of the exosome extractor, the upper surface of the connecting block is inserted with a sampling test tube, the upper surface of the sampling test tube is hingedly connected to the lower surface of the rubber pad, each group of centrifuge test tubes corresponds to a plurality of groups of sampling test tubes, the upper surface of one side of the rubber pad is fixedly connected with a pull handle, and each group of centrifuge test tubes is slidingly connected with a piston.

[0015] As a preferred scheme of the exosome extractor, the profiled frame is internally provided with a toothed belt groove, a transmission toothed belt is rotationally connected in the toothed belt groove, and an arc-shaped limiting plate is fixedly connected in the toothed belt groove.

[0016] As a preferred scheme of the exosome extractor, one end of the connecting shaft is rotationally connected to one side of the linkage plate, a driving motor is fixedly connected to one side of the linkage plate, an auxiliary frame is fixedly connected to one side of the linkage plate, a rotating shaft is fixedly connected to one end of an output shaft of the driving motor, and a belt is rotationally connected to an outer surface of the rotating shaft.

[0017] As a preferred scheme of the exosome extractor, one side of the auxiliary frame is fixedly connected to a support plate, a fixed shaft is rotationally connected to one side of the support plate, a rotating ring is fixedly connected to an outer surface of the fixed shaft, an inner surface of the belt is rotationally connected to an outer surface of the rotating ring, the fixed shaft is fixedly connected to an inner of a transmission wheel, an inclined support plate is fixedly connected to an upper surface of the support plate, and the other end of the inclined support plate is fixedly connected to an outer surface of the driving motor.

[0018] As a preferred scheme of the exosome extractor, a transmission motor is fixedly connected to a lower surface of the profiled frame, a driving gear is fixedly connected to one end of an output shaft of the transmission motor, an inner surface of the transmission toothed belt is meshingly connected to an outer surface of the driving gear, an outer surface of the transmission toothed belt is meshingly connected to an outer surface of the rotating gear, auxiliary moving grooves are formed in both sides of the transmission toothed belt, and an outer surface of the arc-shaped limiting plate is slidingly connected in the auxiliary moving grooves.

[0019] An exosome extractor using method is suitable for any one of the above exosome extractors, and the exosome extractor using method comprises the following steps.

[0020] Step 1: inject the liquid to be extracted into the centrifugal test tube, tightly cover the test tube cover, and ensure that the gas injection tube and the sampling tube are sealed;

[0021] Step 2: the transmission wheel contacts the first rubber wheel, the driving weight frame rotates at a low speed to realize coarse separation;

[0022] Step 3: press the piston to increase the pressure, open the middle layer sampling tube, and the middle layer suspension flows into the sampling test tube through the guide tube;

[0023] Step 4: the transmission motor drives the toothed belt to deform, so that the transmission wheel contacts the second rubber wheel, and the driving weight frame rotates at a medium speed to separate the exosomes;

[0024] Step 5: the piston is pressurized again, the bottom layer sampling tube is opened, and the exosome concentrate flows into the replaced sampling test tube;

[0025] Step 6: The toothed belt continues to deform, the transmission wheel contacts the third rubber wheel, and the highest speed centrifugation further purifies the exosomes;

[0026] Step 7: The sampling test tube containing exosomes is directly taken out, and there is no opening operation throughout the whole process.

[0027] Advantages of the present application:

[0028] 1. The use of rotating rods, first rubber wheels, second rubber wheels, third rubber wheels, bearing frames and centrifugal test tubes enables the adjustment driving unit to change the centrifugal speed quickly during driving according to the different inner diameters of the first rubber wheels, the second rubber wheels and the third rubber wheels, thereby avoiding frequent replacement of different centrifugal devices during extraction.

[0029] 2. The use of centrifugal test tubes, connecting plates, auxiliary rings, auxiliary grooves, link blocks, guide tubes, rubber pads, link tubes, limiting rings, sampling tubes, pistons, test tube covers, gas injection tubes and sampling test tubes enables the centrifugal test tube to hold the product to be processed. When multi-stage centrifugation is performed, the sampling tube of different heights in the test tube is used to extract and process the corresponding suspension in the stratification. At the same time, the gas injection tube and the piston are used to gradually increase the air in the upper space of the centrifugal tube, thereby increasing the pressure above it, and the piston moves downward, thereby accumulating the suspension in each stratification into the sampling tube, thereby quickly sampling the separated liquid to some extent to test its actual centrifugal effect, and then collecting and processing the sample through the sampling test tube.

[0030] 3. The use of connecting shafts, inclined support plates, driving motors, auxiliary frames, belts, support plates, special-shaped frames, support frames, toothed belt grooves, moving grooves and transmission toothed belts enables the rotating gear to move in the moving groove of the special-shaped frame, and at the same time, the transmission toothed belt and the rotating gear are used to cooperate with each other, so that the transmission toothed belt can move quickly in the moving groove on one side of the tooth groove, and at the same time, the arc-shaped limiting plate enables the transmission toothed belt to form a special transmission track, thereby enabling the device to quickly adjust the centrifugal speed to some extent. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can obtain other drawings according to these drawings without creating any creative labor. Among them:

[0032] Figure 1 The overall structure diagram of the exosome extractor proposed by the present application;

[0033] Figure 2 The internal structure diagram of the extraction box of the exosome extractor put forward in the application;

[0034] Figure 3 The centrifugal sampling unit structure diagram of the exosome extractor put forward in the application;

[0035] Figure 4 The overall cross-sectional structure diagram of the centrifugal sampling unit of the exosome extractor put forward in the application;

[0036] Figure 5 The adjustment driving unit structure diagram of the exosome extractor put forward in the application;

[0037] Figure 6 The auxiliary wheel distribution structure diagram of the exosome extractor put forward in the application;

[0038] Figure 7 The transmission gear belt distribution structure diagram of the exosome extractor put forward in the application;

[0039] Figure 8 The flow structure diagram of the exosome extractor put forward in the application.

[0040] BRIEF DESCRIPTION OF DRAWINGS: 100, main unit; 101, extraction box; 102, box door; 103, hinge; 104, shaft seat; 200, centrifugal sampling unit; 201, rotating rod; 202, first rubber wheel; 203, second rubber wheel; 204, third rubber wheel; 205, bearing frame; 206, centrifugal test tube; 207, connecting plate; 208, auxiliary ring; 209, auxiliary groove; 210, adapter block; 211, guide tube; 212, rubber pad; 213, adapter tube; 214, limiting ring; 215, sampling tube; 216, piston; 217, test tube cover; 218, gas injection tube; 219, sampling test tube; 220, extraction handle; 300, adjustment driving unit; 301, connecting shaft; 302, inclined support plate; 303, driving motor; 304, auxiliary frame; 305, belt; 306, support plate; 307, special-shaped frame; 308, support frame; 309, gear belt groove; 310, moving groove; 311, transmission gear belt; 312, auxiliary wheel; 313, fixed shaft; 314, transmission wheel; 315, rotating ring; 316, transmission motor; 317, auxiliary moving groove; 318, rotating gear; 319, linkage plate; 320, rotating shaft; 321, adapter shaft; 322, connecting block; 323, arc-shaped limiting plate; 324, dial gear. DETAILED DESCRIPTION

[0041] In order to make the above-mentioned purposes, features and advantages of the application more obvious and easy to understand, the specific embodiments of the application will be described in detail below with reference to the drawings of the specification.

[0042] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, it will be appreciated that the present application can be practiced in a variety of ways beyond the specific details set forth herein, having regard to the contents of the whole patent document, and that the present application can be practiced in other but essentially similar ways. Accordingly, the present application is not limited in scope to the specific implementations disclosed herein.

[0043] Secondly, the term "one embodiment" or "an embodiment" as used herein means a specific implementation, or a specific feature, structure, or characteristic, that is included in at least one implementation of the present application. The appearances of the term "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily all referring to a single, special implementation, or implementation that is exclusive of all other implementations.

[0044] Thirdly, the present application is described in detail in conjunction with the schematic drawings, and in the detailed description of the embodiments of the present application, the sectional views of the device structure are partially enlarged without the general proportion for the convenience of illustration, and the schematic drawings are only examples, which should not limit the scope of protection of the present application. In addition, the three-dimensional spatial dimensions of length, width and depth should be included in the actual manufacture.

[0045] Embodiment one:

[0046] With reference to Figure 1 - Figure 8 For one embodiment of the present application, an exosome extractor is provided, which comprises a main unit 100, a centrifugal sampling unit 200 and an adjusting driving unit 300.

[0047] The main unit 100 comprises an extraction box 101 and a hinge 103 fixedly connected to one side of the extraction box 101, and the hinge 103 is fixedly connected with a box door 102 on one side, and the shaft seat 104 is fixedly connected inside the extraction box 101;

[0048] Secondly, the centrifugal sampling unit 200 comprises a connecting plate 207 engagedly connected to the inner wall of the extraction box 101 and a rotating rod 201 rotatably connected to the inner surface of the shaft seat 104, the connecting plate 207 is arc-shaped on one side, the connecting plate 207 is rotatably connected with a load-bearing frame 205 on the arc-shaped side, the rotating rod 201 is equidistantly distributed with a first rubber wheel 202, a second rubber wheel 203 and a third rubber wheel 204 on the outer surface, and a plurality of groups of centrifugal test tubes 206 are circumferentially distributed in the load-bearing frame 205;

[0049] Secondly, the adjusting driving unit 300 comprises a support frame 308 fixedly connected to one side of the extraction box 101 and a special-shaped frame 307 fixedly connected to one side of the support frame 308, a transmission wheel 314 frictionally connected to one side of the first rubber wheel 202, a moving groove 310 formed in the special-shaped frame 307, a rotating gear 318 rotatably connected to the moving groove 310, a connecting block 322 fixedly connected to the lower surface of the special-shaped frame 307, a connecting shaft 301 fixedly connected to one side of the connecting block 322, and an auxiliary wheel 312 rotatably connected to the outer surface of the connecting shaft 301.

[0050] Further, the upper surface of the bearing frame 205 is insertedly connected with a connecting block 210, one end of the rotating rod 201 is fixedly connected to the lower surface of the bearing frame 205, the outer surface of the bearing frame 205 is provided with an auxiliary groove 209, and the inner diameter of the auxiliary groove 209 is matched with the connecting plate 207, wherein the contact between the bearing frame 205 and the connecting plate 207 can keep the rotating effect to a certain extent, so that the rotating rod 201 can quickly drive the bearing frame 205 and the connecting block 210 on the upper surface thereof to rotate.

[0051] Further, the lower surface of the bearing frame 205 is fixedly connected with an auxiliary ring 208, the inner surface of the auxiliary ring 208 is rotatably connected with a limiting ring 214, one side of the limiting ring 214 away from the auxiliary ring 208 is fixedly connected to the outer surface of the rotating rod 201, and one end of the centrifugal test tube 206 is clampingly connected with a test tube cover 217, and the test tube cover 217 is fixedly connected with a sampling tube 215 and an air injection tube 218, wherein the bearing frame 205 and the centrifugal test tube 206 can be clampingly disassembled, so that the centrifugal test tube 206 can be disassembled and cleaned to a certain extent, further, the sampling tube 215 and the air injection tube 218 can be used to quickly extract the liquid in the centrifugal test tube 206 and simultaneously increase the pressure at the top of the centrifugal test tube 206.

[0052] Further, the sampling tubes 215 are a plurality of and symmetrically distributed, the orifices of the sampling tubes 215 in the centrifugal test tube 206 are equidistantly distributed, one end of each sampling tube 215 is fixedly connected with a connecting tube 213, one end of the connecting tube 213 is clampingly connected with a guide tube 211, and one end of the guide tube 211 is insertingly connected with a rubber pad 212, wherein the connecting tube 213 and the guide tube 211 are matched with each other, so that the equipment can be separated and treated through the combined structure during disassembly, thereby improving the disassembly of the centrifugal test tube 206 to a certain extent.

[0053] Further, the upper surface of the adapter block 210 is inserted with a sampling test tube 219, the upper surface of the sampling test tube 219 is clamped and connected to the lower surface of the rubber pad 212, and each group of centrifugal test tubes 206 corresponds to a plurality of groups of sampling test tubes 219, the upper surface of one side of the rubber pad 212 is fixedly connected with a lifting handle 220, and each group of centrifugal test tubes 206 is slidingly connected with a piston 216, wherein the sampling test tube 219 is inserted through the insertion structure, so that the sampling test tube 219 can be quickly taken out after each sampling is completed, and further through the movement of the piston 216, the pressure inside the centrifugal test tube 206 can be quickly changed, thereby improving the extraction effect of the sample to a certain extent, collecting the exosome solution of different concentrations into the corresponding sampling test tube, and replacing the sampling test tube after sampling is completed to avoid mixing of the sampling solutions of different concentrations, thereby reducing the influence on separation.

[0054] Further, by using the rotating rod 201, the first rubber wheel 202, the second rubber wheel 203, the third rubber wheel 204, the bearing frame 205 and the centrifugal test tube 206, the adjustment driving unit 300 can change the centrifugal speed quickly during driving according to the different inner diameters of the first rubber wheel 202, the second rubber wheel 203 and the third rubber wheel 204, thereby avoiding frequent replacement of different centrifugal devices during extraction of the device;

[0055] By using the centrifugal test tube 206, the connecting plate 207, the auxiliary ring 208, the auxiliary groove 209, the adapter block 210, the guide pipe 211, the rubber pad 212, the adapter pipe 213, the limiting ring 214, the sampling pipe 215, the piston 216, the test tube cover 217, the gas injection pipe 218 and the sampling test tube 219, the centrifugal test tube 206 can hold the product to be processed, when multi-stage centrifugation is performed, the sampling pipe 215 of different heights in the test tube is used to extract and process the suspension in the corresponding layer, and at the same time, the gas injection pipe 218 and the piston 216 are used to gradually increase the air in the upper space of the centrifugal test tube, thereby increasing the pressure above, and the piston 216 moves downward, thereby accumulating the suspension in each layer into the sampling pipe 215, thereby quickly sampling the separated liquid to a certain extent to test the actual centrifugal effect, and further collecting and processing the sample through the sampling test tube 219;

[0056] The connecting shaft 301, the inclined support plate 302, the driving motor 303, the auxiliary frame 304, the belt 305, the support plate 306, the special-shaped frame 307, the support frame 308, the toothed belt groove 309, the moving groove 310 and the transmission toothed belt 311 are used, so that the rotating gear 318 can move in the moving groove 310 in the special-shaped frame 307, and at the same time, the transmission toothed belt 311 can be quickly moved in the moving groove 310 under the cooperation of the moving groove 310 on one side of the toothed groove, and at the same time, the transmission toothed belt 311 can form a special transmission track by using the arc-shaped limiting plate, so that the equipment can be quickly adjusted to a centrifugal speed to a certain extent.

[0057] A kind of exosome extractor use method, a kind of exosome extractor use method is suitable for any kind of exosome extractor above, and a kind of exosome extractor use method includes the following steps:

[0058] Step 1: inject the liquid to be extracted into the centrifugal test tube 206, cover the test tube cover 217, ensure that the gas injection pipe 218 and the sampling pipe 215 are sealed;

[0059] Step 2: the transmission wheel 314 contacts the first rubber wheel 202, drives the heavy frame 205 to rotate at low speed, realizes coarse separation;

[0060] Step 3: press the piston 216 to increase pressure, open the middle layer sampling pipe 215, and the middle layer suspension flows into the sampling test tube 219 through the guide pipe 211;

[0061] Step 4: the transmission motor 316 pushes the toothed belt to deform, so that the transmission wheel 314 contacts the second rubber wheel 203, and the heavy frame 205 rotates at medium speed to separate the exosome;

[0062] Step 5: the piston 216 is pressurized twice, the bottom layer sampling pipe 215 is opened, and the exosome concentrate flows into the replaced sampling test tube 219;

[0063] Step 6: the toothed belt continues to deform, the transmission wheel 314 contacts the third rubber wheel 204, and the exosome is further purified at the highest speed;

[0064] Step 7: directly take out the sampling test tube 219 containing exosome, without opening the cover operation.

[0065] Example two

[0066] Reference Figure 2 And Figure 5 - Figure 7The difference between the embodiment one and the embodiment two is that the toothed belt groove 309 is arranged in the special-shaped frame 307, the transmission toothed belt 311 is rotatably connected in the toothed belt groove 309, the arc-shaped limiting plate 323 is fixedly connected in the toothed belt groove 309, the connecting shaft 301 is rotatably connected to the outer surface of the transmission toothed belt 311, the one side of the rotating gear 318 is fixedly connected with the connecting shaft 321, wherein the toothed groove is arranged in the moving groove 310 in the special-shaped frame 307 and is meshed with the rotating gear 318, the rotating gear 318 is meshed and moved through the toothed groove in the moving groove 310, so that the position of the transmission wheel 314 is changed.

[0067] Further, the one end of the connecting shaft 321 is rotatably connected to the one side of the linkage plate 319, the one side of the linkage plate 319 is fixedly connected with the driving motor 303, the one side of the linkage plate 319 is fixedly connected with the auxiliary frame 304, the one end of the output shaft of the driving motor 303 is fixedly connected with the rotating shaft 320, the outer surface of the rotating shaft 320 is rotatably connected with the belt 305, wherein the belt 305 is driven through the driving motor 303, so that the belt 305 drives the transmission wheel 314 to synchronously rotate, and the linkage plate 319 is bent and moved under the synchronous driving of the rotating gear 318.

[0068] Further, the one side of the auxiliary frame 304 is fixedly connected with the supporting plate 306, the one side of the supporting plate 306 is rotatably connected with the fixed shaft 313, the outer surface of the fixed shaft 313 is fixedly connected with the rotating ring 315, the outer surface of the rotating ring 315 is rotatably connected to the inner surface of the belt 305, the outer surface of the fixed shaft 313 is fixedly connected in the transmission wheel 314, the upper surface of the supporting plate 306 is fixedly connected with the inclined supporting plate 302, the other end of the inclined supporting plate 302 is fixedly connected to the outer surface of the driving motor 303, wherein the driving motor 303 is not hindered by the rubber wheel in the centrifugal sampling unit 200 in the transmission process through the arrangement of the supporting plate 306, and the movement effect is not affected.

[0069] Further, the lower surface of the special-shaped frame 307 is fixedly connected with the transmission motor 316, the one end of the output shaft of the transmission motor 316 is fixedly connected with the driving gear 324, the outer surface of the driving gear 324 is meshingly connected to the inner surface of the transmission toothed belt 311, the outer surface of the transmission toothed belt 311 is meshingly connected to the outer surface of the rotating gear 318, and the transmission toothed belt 311 is provided with the auxiliary moving groove 317 on both sides, and the outer surface of the arc-shaped limiting plate 323 is slidably connected in the auxiliary moving groove 317, wherein the transmission toothed belt 311 is driven by the transmission motor 316 through the driving gear 324, and the shape of the transmission toothed belt 311 is limited through the cooperation of the arc-shaped limiting plate 323 and the auxiliary moving groove 317.

[0070] Working principle: first, the extraction box 101 and the box door 102 through the hinge 103 to form a closed space, to avoid the invasion of external bacteria, shaft seat 104 rigid support rotating rod 201, eliminate the vibration displacement when high speed centrifugation;

[0071] Second, the diameter difference between the first rubber wheel 202, the second rubber wheel 203 and the third rubber wheel 204 form three level gear, transmission wheel 314 selective contact different rubber wheel, change the rotating speed of the load frame 205, centrifugal test tube 206 is equipped with multiple height sampling tube 215, corresponding to different density material layering, the piston 216 down, gas injection pipe 218 injection gas boost, each layer of suspension through the corresponding sampling tube 215 into the guide pipe 211, the adapter block 210 and rubber pad 212 form a sealed interface, the sample through the adapter tube 213 directly into the sampling test tube 219, the whole process without exposure;

[0072] Then, the transmission motor 316 drive the gear 324, drive gear belt 311 in the tooth belt groove 309 moves, arc limiting plate 323 constraint tooth belt trajectory, make it bend deformation, tooth belt drive rotating gear 318 along the moving groove 310 slide, rotating gear 318 through the shaft 321 linkage transmission wheel 314 transverse displacement, switch contact different rubber wheel, drive motor 303 through the belt 305 drive transmission wheel 314 rotation, support plate 306 and inclined support plate 302 constitute a triangular stable support, inhibit the centrifugal vibration.

[0073] It should be noted that the above examples are used to illustrate the technical solutions of the present application rather than limiting, although the present application is described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by the equivalent, without departing from the spirit and scope of the technical solutions of the present application, which should be covered in the scope of the claims of the present application.

Claims

1. An exosome extractor, characterized by, The exosome extractor comprises: A main unit (100) comprising an extraction box (101) and a hinge (103) fixedly connected to one side of the extraction box (101), one side of the hinge (103) being fixedly connected with a box door (102), and an axle seat (104) fixedly connected inside the extraction box (101); A centrifugal sampling unit (200) comprising a connecting plate (207) clamped to the inner wall of the extraction box (101) and a rotating rod (201) rotatably connected to the inner surface of the axle seat (104), one side of the connecting plate (207) being in an arc shape, the arc-shaped side of the connecting plate (207) being rotatably connected with a load-bearing frame (205), the outer surface of the rotating rod (201) being equidistantly provided with a first rubber wheel (202), a second rubber wheel (203) and a third rubber wheel (204), and a plurality of groups of centrifugal test tubes (206) being circumferentially distributed in the load-bearing frame (205). The adjusting driving unit (300) includes the support frame (308) fixedly connected with the extraction box (101) on one side and the special-shaped frame (307) fixedly connected on one side of the support frame (308), the first rubber wheel (202) is frictionally connected with the transmission wheel (314) on one side, the special-shaped frame (307) is provided with the moving groove (310) in the inside, the rotating gear (318) is rotatably connected in the moving groove (310), the connecting block (322) is fixedly connected to the lower surface of the special-shaped frame (307), the connecting shaft (301) is fixedly connected on one side of the connecting block (322), the auxiliary wheel (312) is rotatably connected to the outer surface of the connecting shaft (301), the special-shaped frame (307) is provided with the toothed belt groove (309) in the inside, the transmission toothed belt (311) is rotatably connected in the toothed belt groove (309), the arc-shaped limiting plate (323) is fixedly connected in the toothed belt groove (309), the transmission toothed belt (311) is rotatably connected to the outer surface of the connecting shaft (301), one side of the rotating gear (318) is fixedly connected with the connecting shaft (321), one end of the connecting shaft (321) is rotatably connected on one side of the linkage plate (319), the driving motor (303) is fixedly connected on one side of the linkage plate (319), the auxiliary frame (304) is fixedly connected on one side of the linkage plate (319), one end of the output shaft of the driving motor (303) is fixedly connected with the rotating shaft (320), the rotating shaft (320) is rotatably connected with the belt (305) on the outer surface, the support plate (306) is fixedly connected on one side of the auxiliary frame (304), the fixed shaft (313) is rotatably connected on one side of the support plate (306), the rotating ring (315) is fixedly connected to the outer surface of the fixed shaft (313), the rotating ring (315) is rotatably connected to the inner surface of the belt (305), the fixed shaft (313) is fixedly connected in the transmission wheel (314), the inclined supporting plate (302) is fixedly connected to the upper surface of the support plate (306), the other end of the inclined supporting plate (302) is fixedly connected to the outer surface of the driving motor (303), the transmission motor (316) is fixedly connected to the lower surface of the special-shaped frame (307), the driving gear (324) is fixedly connected to one end of the output shaft of the transmission motor (316), the driving gear (324) is meshedly connected to the inner surface of the transmission toothed belt (311), the transmission toothed belt (311) is meshedly connected to the outer surface of the rotating gear (318), and the transmission toothed belt (311) is provided with the auxiliary moving grooves (317) on both sides, and the arc-shaped limiting plate (323) is slidably connected in the auxiliary moving grooves (317).

2. The exosome extractor of claim 1, wherein: The upper surface of the bearing frame (205) is insertedly connected with the connecting block (210), one end of the rotating rod (201) is fixedly connected to the lower surface of the bearing frame (205), and the outer surface of the bearing frame (205) is provided with the auxiliary groove (209) which is matched with the connecting plate (207) in the inner diameter.

3. The exosome extractor of claim 2, wherein: The lower surface of the load frame (205) is fixedly connected with an auxiliary ring (208), the inner surface of the auxiliary ring (208) is rotatably connected with a limiting ring (214), the side, away from the auxiliary ring (208), of the limiting ring (214) is fixedly connected with the outer surface of the rotating rod (201), one end of the centrifugal tube (206) is clampedly connected with a test tube cover (217), and the inner surface of the test tube cover (217) is fixedly connected with a sampling tube (215) and an air injection tube (218) respectively.

4. The exosome extractor of claim 3, wherein: The sampling tubes (215) are in number of several and are symmetrically distributed, the orifices of the sampling tubes (215) inside the centrifugal tube (206) are equidistantly distributed, one end of the sampling tube (215) is fixedly connected with a connecting tube (213), one end of the connecting tube (213) is clampedly connected with a guide tube (211), and one end of the guide tube (211) is insertedly connected with a rubber pad (212).

5. The exosome extractor of claim 4, wherein: The upper surface of the connecting block (210) is inserted with a sampling test tube (219), the upper surface of the sampling test tube (219) is clampedly connected with the lower surface of the rubber pad (212), and each group of centrifugal tubes (206) corresponds to a plurality of groups of sampling test tubes (219), the upper surface of one side of the rubber pad (212) is fixedly connected with a pull handle (220), and each group of centrifugal tubes (206) is slidably connected with a piston (216).

6. A method of use of an exosome extractor, the method of use of an exosome extractor of claim 5, wherein, The use method of the exosome extractor includes the following steps: Step 1: inject the liquid to be extracted into the centrifugal tube (206), tightly cover the test tube cover (217), and ensure that the air injection tube (218) and the sampling tube (215) are sealed; Step 2: the transmission wheel (314) contacts the first rubber wheel (202), the load frame (205) is driven to rotate at low speed, and coarse separation is realized; Step 3: press down the piston (216) to increase the pressure, open the middle layer sampling tube (215), and the middle layer suspension flows into the sampling test tube (219) through the guide tube (211); Step 4: the transmission motor (316) drives the toothed belt to deform, so that the transmission wheel (314) contacts the second rubber wheel (203), the load frame (205) rotates at medium speed to separate the exosomes; Step 5: the piston (216) is pressurized again, the bottom layer sampling tube (215) is opened, and the exosome concentrate flows into the replaced sampling test tube (219); Step 6: the toothed belt continues to deform, the transmission wheel (314) contacts the third rubber wheel (204), and the highest speed centrifugation further purifies the exosomes; Step 7: directly take out the sampling test tube (219) containing the exosomes, and there is no opening operation in the whole process.

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