Preparation method of bone repair material based on bovine bone

Abstract

The invention relates to the technical field of bone repair materials, and discloses a preparation method of a bone repair material based on bovine bones, which comprises the following preparation steps: 1) cleaning the bovine bones; (2) cancellous bones of the cattle bones are cut out; (3) repeatedly washing the cancellous bone; 4) boiling the cancellous bone, cutting the cancellous bone into bone blocks, and drying the bone blocks; 5) degreasing the bone blocks and then drying the bone blocks; (6) carrying out gradient deproteinization treatment on the bone blocks; 7) soaking the bone blocks in an alkali liquor solvent to remove the deproteinization solvent; 8) performing low-temperature calcination treatment, crushing treatment and screening treatment on the bone blocks to obtain the bone repair material. In the preparation step (3), the cancellous bone is placed in a flusher, the flusher is internally provided with a rotating cage and an air blowing structure, the rotating cage drives the cancellous bone to rotate, the air blowing structure blows air in multiple directions, water is driven to flush the cancellous bone, the cancellous bone is driven to be in an up-down floating state on the rotating cage, and therefore the cancellous bone achieves a better flushing effect.

Description

Technical Field

[0001] This invention relates to the technical field of bone repair materials, and more specifically, to a method for preparing bone repair materials based on bovine bone. Background Technology

[0002] In the field of bone repair material preparation, cancellous bone, as an important raw material, is widely used for bone tissue replacement and repair due to its porous structure and good bioactivity. However, the rinsing process is one of the key technologies in the preparation of cancellous bone, which can directly affect the purity of bone repair materials and the subsequent clinical application effect.

[0003] In existing technologies, when rinsing cancellous bone using a single method, the water flow can only act on the surface of the cancellous bone and cannot fully penetrate the internal microporous structure. This results in the inability to completely remove residual blood and other impurities, affecting the biocompatibility of the bone and the effectiveness of subsequent treatments. Traditional rinsing equipment mostly uses static soaking, where cancellous bone remains stationary or only passively receives water during rinsing. Due to the lack of active turning or mechanical vibration assistance, the uniformity and efficiency of rinsing are significantly limited. In addition, the lack of multi-directional rinsing function makes it impossible to achieve full-angle coverage of cancellous bone by water flow. This means that residues in some areas may remain inside the bone for a long time, further affecting the rinsing effect. Summary of the Invention

[0004] The purpose of this invention is to provide a method for preparing bone repair materials based on bovine bone, aiming to solve the problem of poor rinsing effect on cancellous bone during the preparation of bone repair materials in the prior art.

[0005] This invention is achieved through a method for preparing bone repair materials based on bovine bone, comprising the following preparation steps: 1) Clean the beef bones to remove grease and soft tissue; 2) The bovine bone is cut to remove the cancellous bone. 3) Soak the cancellous bone in water and rinse it repeatedly to remove the blood from the cancellous bone; 4) Place the cancellous bone in water and heat the water to boiling to boil the cancellous bone; cut the boiled cancellous bone into blocks to form bone blocks, and dry the bone blocks. 5) Soak the bone blocks in a degreasing solvent for a set time to degrease the bone blocks, and then dry the degreased bone blocks. 6) Immerse the bone blocks in a deproteinizing solvent to perform a gradient deproteinization treatment on the bone blocks; 7) Soak the bone blocks in an alkaline solvent to remove the deproteinizing solvent remaining on the bone blocks; 8) The bone blocks are subjected to low-temperature calcination, crushing and sieving in sequence to obtain bone repair material.

[0006] In preparation step 3), the cancellous bone is placed in an rinsing device. The rinsing device has a rinsing chamber. The bottom of the rinsing chamber is provided with a rotating disk. The rinsing chamber is provided with a horizontally rotating cage. The outer periphery and bottom of the rotating cage are provided with multiple hollow holes. The bottom of the rotating cage is arranged facing the rotating disk. The outside of the rotating cage is provided with an air blowing structure. The air blowing structure blows air towards the rotating cage in multiple directions. When the cancellous bone is placed in the rotating cage, the rotating cage causes the cancellous bone to rotate, and the air blowing structure blows air in multiple directions in the water, causing the water to wash the cancellous bone in multiple directions, and causing the cancellous bone to float up and down on the rotating cage.

[0007] Furthermore, in preparation step 5), the degreasing solvent is one or more of ethanol, methanol, chloroform, diethyl ether, petroleum ether, and acetone, and the bone block is soaked in the degreasing solvent for 12 to 24 hours.

[0008] Furthermore, in preparation step 6), the deproteinizing solvent is an ethylenediamine solution, and the concentration of the ethylenediamine solution is between 30% and 80%.

[0009] Furthermore, in preparation step 7), the alkaline solvent is one or more of sodium hydroxide, potassium hydroxide, calcium carbonate, and sodium bicarbonate, and the concentration of the alkaline solvent is 8 g / L to 15 g / L.

[0010] Furthermore, in the preparation step 3), during the repeated rinsing of the cancellous bone in the water, the rotating cage reciprocates with varying direction and speed, and the air pressure of the air blowing structure is in a state of change.

[0011] Furthermore, in the preparation step 8), the temperature of the low-temperature calcination treatment is between 300℃ and 400℃, and the treatment time of the low-temperature calcination treatment is between 10 hours and 15 hours.

[0012] Furthermore, in the preparation step 8), the particle size of the obtained bone repair material is between 0.3 mm and 1.2 mm.

[0013] Furthermore, in the preparation step 3), the blowing structure includes a plurality of longitudinally arranged peripheral air pipes and a bottom air pipe. The plurality of peripheral air pipes are arranged at intervals around the circumference of the rotating cage, and the plurality of peripheral air pipes enclose a surrounding area, in which the rotating cage is located. The outer peripheral air pipe surrounds the outer periphery of the rotating cage. The outer peripheral air pipe is provided with a plurality of outer peripheral air holes, which are arranged facing the surrounding area and spaced apart along the height direction of the outer peripheral air pipe. The bottom air pipe is located below the rotating cage. The bottom air pipe is provided with a plurality of bottom air holes, which are arranged from bottom to top facing the bottom of the rotating cage. In preparation step 3), after the cancellous bone is placed in the rotating cage, the rotating cage rotates horizontally, the outer air blowing holes blow air towards the outer periphery of the rotating cage to form an outer airflow, and the bottom air blowing holes blow air towards the bottom of the rotating cage to form a bottom airflow. The outer airflow and the bottom airflow drive the water to rinse the cancellous bone in multiple directions, and cause the cancellous bone to float up and down in the rotating cage.

[0014] Furthermore, in preparation step 3), the bottom air pipe is horizontally spiraled and abuts against the rotating disk. The direction of the horizontal spiral of the bottom air pipe is different from the direction of rotation of the rotating disk. Multiple bottom air guide pipes are connected to the bottom air outlet. The lower end of the bottom air guide pipe is connected to the bottom air pipe and communicates with the bottom air blowing hole. The upper end of the bottom air guide pipe extends towards the bottom of the rotating cage. The bottom air guide pipe is inclined, and the inclination directions of the multiple bottom air guide pipes are different. The peripheral air tube is provided with multiple peripheral air guide tubes. The outer end of the peripheral air guide tube is connected to the peripheral air tube and communicates with the peripheral air blowing hole. The inner end of the peripheral air guide tube extends towards the outer periphery of the rotating cage. The peripheral air guide tubes are arranged at an angle, and the angles of the multiple peripheral air guide tubes are different. In the preparation step 3), multiple peripheral air holes blow out multiple peripheral airflows through peripheral air guide pipes. The multiple peripheral airflows are directed toward the outer periphery of the rotating cage, and the blowing directions of the multiple peripheral airflows are different. Multiple bottom air holes blow out multiple bottom airflows through bottom air pipes. These multiple bottom airflows are directed toward the bottom of the rotating cage and blow in different directions. The multiple peripheral airflows and multiple bottom airflows drive water to rinse the cancellous bone in multiple directions and cause the cancellous bone to float up and down in the rotating cage.

[0015] Furthermore, in the preparation step 4), the cancellous bone is placed in a heating furnace, the heating furnace having a furnace cavity containing water; the furnace cavity is provided with a suspended metal mesh cover, the mesh cover having a mesh cavity with a top opening, and the mesh cover having multiple mesh holes; The outer periphery of the mesh cover is connected to multiple elastic sheets. The outer ends of the elastic sheets are fixedly connected to the inner sidewall of the furnace cavity, and the inner ends of the elastic sheets are connected to the outer periphery of the mesh cover. The multiple elastic sheets are arranged at intervals around the circumference of the mesh cover, and the middle part of the elastic sheets is bent downward. In preparation step 4), the cancellous bone is placed in the mesh cavity, the water body is submerged over the mesh cover and multiple elastic sheets, the water body is heated to a boiling state, the water body boils the cancellous bone, and the cancellous bone is driven up and down by the boiling water body, and the multiple elastic sheets synchronously drive the mesh cover up and down, so that the cancellous bone in the mesh cavity is in a floating state.

[0016] Compared with the prior art, the method for preparing bone repair materials based on bovine bone provided by the present invention, when cancellous bone is placed in a rotating cage, the rotating cage drives it to rotate. The hollow holes of the rotating cage, combined with the multi-directional air supply of the blowing structure, allow water to impact the cancellous bone from all directions, deeply clean the micropores, and remove impurities that were previously difficult to reach. At the same time, the cancellous bone is caused to float up and down in the rotating cage, allowing dynamic water to continuously penetrate into the bone block. This process breaks the static limitations of traditional rinsing, thereby eliminating cleaning blind spots. It not only improves cleaning efficiency, but also provides high-quality bone blocks for subsequent degreasing, deproteinization and other processes, ultimately improving the overall performance and biocompatibility of bone repair materials. Attached Figure Description

[0017] Figure 1 This is a schematic flowchart of the method for preparing bone repair materials based on bovine bone provided by the present invention; Figure 2 This is a cross-sectional schematic diagram of the flushing device provided by the present invention; Figure 3 This is a cross-sectional schematic diagram of the heating furnace provided by the present invention; In the diagram: flusher 100, flushing chamber 101, rotating disk 102, rotating interval 103, rotating shaft 104, water body 105; Rotating cage 200, outer trachea 201, outer trachea 202, bottom trachea 203, bottom trachea 204; Heating furnace 300, furnace cavity 301, mesh cover 302, elastic sheet 303. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0019] The implementation of the present invention will be described in detail below with reference to specific embodiments.

[0020] In the accompanying drawings of this embodiment, the same or similar reference numerals correspond to the same or similar components. In the description of this invention, it should be understood that if terms such as "upper," "lower," "left," and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting this invention. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0021] Reference Figure 1-3 The image shows a preferred embodiment of the present invention.

[0022] The method for preparing bone repair materials based on bovine bone includes the following preparation steps: 1) Clean the beef bones to remove grease and soft tissue; 2) Cut the cattle bones to remove the cancellous bone. 3) Soak the cancellous bone in water at 105 and rinse repeatedly to remove the blood from the cancellous bone; 4) Place the cancellous bone in water 105 and heat water 105 to boiling so that the cancellous bone is boiled in water 105; cut the boiled cancellous bone into blocks to form bone blocks, and dry the bone blocks. 5) Soak the bone pieces in a degreasing solvent for a set time to degrease them, and then dry the degreased bone pieces. 6) Soak the bone blocks in a deproteinizing solvent to perform a gradient deproteinization treatment on the bone blocks; 7) Soak the bone pieces in an alkaline solvent to remove the deproteinizing solvent remaining on the bone pieces; 8) The bone blocks are subjected to low-temperature calcination, crushing and sieving in sequence to obtain bone repair materials.

[0023] In preparation step 3), cancellous bone is placed in rinsing device 100. Rinsing device 100 has rinsing chamber 101. A rotating disk 102 is provided at the bottom of rinsing chamber 101. A horizontally rotating cage 200 is provided in rinsing chamber 101. Multiple hollow holes are provided on the outer periphery and bottom of rotating cage 200. The bottom of rotating cage 200 is arranged facing rotating disk 102. An air blowing structure is provided on the outside of rotating cage 200. The air blowing structure blows air in multiple directions toward rotating cage 200. When the cancellous bone is placed in the rotating cage 200, the rotating cage 200 causes the cancellous bone to rotate, and the air blowing structure blows air in the water body 105 in multiple directions, causing the water body 105 to wash the cancellous bone in multiple directions, and causing the cancellous bone to float up and down on the rotating cage 200.

[0024] The above-mentioned method for preparing bone repair materials based on bovine bone involves placing cancellous bone in a rotating cage 200, which then rotates the cancellous bone. The hollowed-out holes of the rotating cage 200, combined with the multi-directional air supply of the blowing structure, allow water 105 to impact the cancellous bone from all directions, deeply cleaning the micropores and removing impurities that were previously difficult to reach. At the same time, the cancellous bone is caused to float up and down in the rotating cage 200, allowing the dynamic water 105 to continuously penetrate into the bone block. This process breaks the static limitations of traditional rinsing, thereby eliminating cleaning blind spots. It not only improves cleaning efficiency, but also provides high-quality bone blocks for subsequent degreasing, deproteinization and other processes, ultimately improving the overall performance and biocompatibility of bone repair materials.

[0025] In this embodiment, in preparation step 5), the degreasing solvent is one or more of ethanol, methanol, chloroform, diethyl ether, petroleum ether, and acetone, and the bone blocks are soaked in the degreasing solvent for 12 to 24 hours.

[0026] After prolonged soaking, the internal oils of the bone blocks are thoroughly removed, ensuring a complete degreasing effect and thus improving the biocompatibility of the bone repair material.

[0027] In this embodiment, in preparation step 6), the deproteinizing solvent is an ethylenediamine solution with a concentration between 30% and 80%. This concentration range of ethylenediamine solution, and the use of a gradient deproteinization method, can efficiently remove protein from the bone block while avoiding over-processing that could damage the bone block structure.

[0028] In this embodiment, in preparation step 7), the alkaline solvent is one or more of sodium hydroxide, potassium hydroxide, calcium carbonate, and sodium bicarbonate, and the concentration of the alkaline solvent is 8 g / L to 15 g / L.

[0029] During the preparation of bone repair materials, residual deproteinizing solvents can affect the chemical stability and biosafety of the materials. However, bone blocks treated with alkaline solvents have clean surfaces and stable chemical properties, ensuring the safety and effectiveness of bone repair materials.

[0030] In this embodiment, during the preparation step 3), as the cancellous bone is repeatedly rinsed in water 105, the rotating cage 200 rotates back and forth with varying speed and direction, and the air pressure of the air blowing structure is in a state of change.

[0031] This dynamic rinsing method enhances the impact of the water on the cancellous bone, allowing the water to penetrate deep into the microporous structure of the cancellous bone, resulting in higher internal cleanliness. This provides a high-quality foundation for subsequent processes such as boiling, degreasing, and deproteinization, thereby improving the overall quality of the bone repair material.

[0032] In this embodiment, in preparation step 8), the low-temperature calcination temperature is between 300℃ and 400℃, and the calcination time is between 10 and 15 hours. This promotes the formation of a uniform and stable porous structure in the bone blocks, and also increases their specific surface area. Compared with high-temperature long-term treatment, it better retains bioactive components. In this embodiment, in preparation step 8), the particle size of the bone repair material obtained is between 0.3 mm and 1.2 mm; such a bone repair material has good filling properties, and the appropriate particle size can make close contact with the surrounding bone tissue, promote bone integration, and improve the repair effect.

[0033] In this embodiment, in preparation step 3), the blowing structure includes a plurality of longitudinally arranged peripheral air pipes 201 and a bottom air pipe 203. The plurality of peripheral air pipes 201 are arranged around the circumferential space of the rotating cage 200, and the plurality of peripheral air pipes 201 enclose a region, and the rotating cage 200 is located in the enclosed region. The peripheral air pipe 201 surrounds the outer periphery of the rotating cage 200. The peripheral air pipe 201 is provided with multiple peripheral air holes, which are arranged facing the enclosed area and spaced apart along the height direction of the peripheral air pipe 201. The bottom air pipe 203 is located below the rotating cage 200. The bottom air pipe 203 is provided with multiple bottom air holes, which are arranged from bottom to top facing the bottom of the rotating cage 200. In preparation step 3), after the cancellous bone is placed in the rotating cage 200, the rotating cage 200 rotates horizontally, the outer air blowing holes blow air towards the outer periphery of the rotating cage 200 to form an outer airflow, and the bottom air blowing holes blow air towards the bottom of the rotating cage 200 to form a bottom airflow. The outer airflow and the bottom airflow drive the water body 105 to rinse the cancellous bone in multiple directions, and cause the cancellous bone to float up and down in the rotating cage 200.

[0034] The design of multi-directional air blowing and floating rinsing can thoroughly clean cancellous bone, especially effectively removing impurities from the internal micropores. In the preparation of bone repair materials, high internal micropore cleanliness can improve the bioactivity of the material and promote bone tissue growth. Therefore, after the above rinsing, the cancellous bone has a clear internal micropore structure with no impurities remaining.

[0035] In this embodiment, in preparation step 3), the bottom air pipe 203 is horizontally spiraled and abuts against the rotating disk 102. The direction of the horizontal spiral of the bottom air pipe 203 is different from the direction of rotation of the rotating disk 102. Multiple bottom air guide pipes 204 are connected to the bottom air outlet. The lower end of the bottom air guide pipe 204 is connected to the bottom air pipe 203 and communicates with the bottom air blowing hole. The upper end of the bottom air guide pipe 204 extends towards the bottom of the rotating cage 200. The bottom air guide pipes 204 are inclined, and the inclination directions of the multiple bottom air guide pipes 204 are different. The peripheral air tube 201 is provided with multiple peripheral air guide tubes 202. The outer end of the peripheral air guide tube 202 is connected to the peripheral air tube 201 and communicates with the peripheral air blowing hole. The inner end of the peripheral air guide tube 202 extends towards the outer periphery of the rotating cage 200. The peripheral air guide tubes 202 are arranged at an angle, and the angles of the multiple peripheral air guide tubes 202 are different. In preparation step 3), multiple peripheral air holes blow out multiple peripheral air streams through peripheral air guide pipe 202. The multiple peripheral air streams are directed toward the outer periphery of rotating cage 200, and the blowing directions of the multiple peripheral air streams are different. Multiple bottom air holes blow out multiple bottom airflows through the bottom air guide pipe 204. The multiple bottom airflows are directed toward the bottom of the rotating cage 200 and blow in different directions. The multiple peripheral airflows and multiple bottom airflows drive the water body 105 to rinse the cancellous bone in multiple directions and cause the cancellous bone to float up and down in the rotating cage 200.

[0036] By setting up a bottom air duct 204 and an outer peripheral air duct 202, multiple airflows with different directions are generated, which further enhances the impact and rinsing effect of the water flow on the cancellous bone, significantly improves the cleanliness of the cancellous bone, reduces the risk of impurity residue, and improves the quality and safety of bone repair materials.

[0037] In this embodiment, the bottom of the rotating disk 102 and the bottom of the rinsing chamber 101 have a rotational interval 103. The bottom of the rotating disk 102 is connected to a rotating shaft 104, which is arranged off-center from the center of the rotating disk 102. The rotating shaft 104 is connected to a motor and is equipped with a switch. When the cancellous bone is placed in the rotating cage 200, the motor can be turned on according to the rinsing needs of the cancellous bone. The rotating shaft 104 drives the rotating disk 102 to rotate synchronously, thereby increasing the airflow when blowing air through the bottom air pipe 204 and realizing the control of the rinsing effect.

[0038] In this embodiment, in preparation step 4), the cancellous bone is placed in a heating furnace 300. The heating furnace 300 has a furnace cavity 301, and water 105 is installed in the furnace cavity 301. A metal suspended mesh cover 302 is provided in the furnace cavity 301. The mesh cover 302 has a mesh cavity with a top opening and multiple mesh holes. Multiple elastic pieces 303 are connected to the outer periphery of the mesh cover 302. The outer ends of the elastic pieces 303 are fixedly connected to the inner side wall of the furnace cavity 301, and the inner ends of the elastic pieces 303 are connected to the outer periphery of the mesh cover 302. The multiple elastic pieces 303 are arranged around the mesh cover 302 at intervals in the circumferential direction, and the middle part of the elastic pieces 303 is bent downward. In preparation step 4), the cancellous bone is placed in the mesh cavity, and the water 105 is submerged over the mesh cover 302 and multiple elastic sheets 303. After the water 105 is heated to a boiling state, the water 105 boils the cancellous bone, and the cancellous bone is driven to float up and down by the boiling water 105. The multiple elastic sheets 303 synchronously drive the mesh cover 302 to float up and down, so that the cancellous bone in the mesh cavity is in a floating state.

[0039] During the boiling process, the cancellous bone is heated evenly and thoroughly, which reduces the content of internal impurities and microorganisms, improves the safety of the material, and provides high-quality raw materials for subsequent processes such as degreasing and deproteinization.

[0040] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preparing bone repair materials based on bovine bone, characterized in that, The preparation steps include the following: 1) Clean the beef bones to remove grease and soft tissue; 2) The bovine bone is cut to remove the cancellous bone. 3) Soak the cancellous bone in water and rinse it repeatedly to remove the blood from the cancellous bone; 4) Place the cancellous bone in water and heat the water to boiling to boil the cancellous bone; cut the boiled cancellous bone into blocks to form bone blocks, and dry the bone blocks. 5) Soak the bone blocks in a degreasing solvent for a set time to degrease the bone blocks, and then dry the degreased bone blocks. 6) Immerse the bone blocks in a deproteinizing solvent to perform a gradient deproteinization treatment on the bone blocks; 7) Soak the bone blocks in an alkaline solvent to remove the deproteinizing solvent remaining on the bone blocks; 8) The bone blocks are subjected to low-temperature calcination, pulverization and sieving in sequence to obtain bone repair material; In preparation step 3), the cancellous bone is placed in an rinsing device. The rinsing device has a rinsing chamber. The bottom of the rinsing chamber is provided with a rotating disk. The rinsing chamber is provided with a horizontally rotating cage. The outer periphery and bottom of the rotating cage are provided with multiple hollow holes. The bottom of the rotating cage is arranged facing the rotating disk. The outside of the rotating cage is provided with an air blowing structure. The air blowing structure blows air towards the rotating cage in multiple directions. When the cancellous bone is placed in the rotating cage, the rotating cage causes the cancellous bone to rotate, and the air blowing structure blows air in multiple directions in the water, causing the water to wash the cancellous bone in multiple directions, and causing the cancellous bone to float up and down on the rotating cage.

2. The method for preparing bone repair material based on bovine bone as described in claim 1, characterized in that, In preparation step 5), the degreasing solvent is one or more of ethanol, methanol, chloroform, diethyl ether, petroleum ether, and acetone, and the bone block is soaked in the degreasing solvent for 12 to 24 hours.

3. The method for preparing bone repair material based on bovine bone as described in claim 1, characterized in that, In preparation step 6), the deproteinizing solvent is an ethylenediamine solution, and the concentration of the ethylenediamine solution is between 30% and 80%.

4. The method for preparing bone repair material based on bovine bone as described in claim 1, characterized in that, In preparation step 7), the alkaline solvent is one or more of sodium hydroxide, potassium hydroxide, calcium carbonate, and sodium bicarbonate, and the concentration of the alkaline solvent is 8 g / L to 15 g / L.

5. The method for preparing bone repair material based on bovine bone as described in claim 1, characterized in that, In preparation step 3), during the repeated rinsing of the cancellous bone in water, the rotating cage reciprocates with varying direction and speed, and the air pressure of the air blowing structure is in a state of change.

6. The method for preparing bone repair material based on bovine bone as described in claim 1, characterized in that, In preparation step 8), the temperature of the low-temperature calcination treatment is between 300℃ and 400℃, and the treatment time is between 10 hours and 15 hours.

7. The method for preparing bone repair material based on bovine bone as described in claim 1, characterized in that, In preparation step 8), the particle size of the obtained bone repair material is between 0.3 mm and 1.2 mm.

8. The method for preparing bovine bone repair material according to any one of claims 1-7, characterized in that, In the preparation step 3), the air blowing structure includes a plurality of longitudinally arranged peripheral air pipes and a bottom air pipe. The plurality of peripheral air pipes are arranged at intervals around the circumference of the rotating cage, and the plurality of peripheral air pipes enclose a region, in which the rotating cage is located. The outer peripheral air pipe surrounds the outer periphery of the rotating cage. The outer peripheral air pipe is provided with a plurality of outer peripheral air holes, which are arranged facing the surrounding area and spaced apart along the height direction of the outer peripheral air pipe. The bottom air pipe is located below the rotating cage. The bottom air pipe is provided with a plurality of bottom air holes, which are arranged from bottom to top facing the bottom of the rotating cage. In preparation step 3), after the cancellous bone is placed in the rotating cage, the rotating cage rotates horizontally, the outer air blowing holes blow air towards the outer periphery of the rotating cage to form an outer airflow, and the bottom air blowing holes blow air towards the bottom of the rotating cage to form a bottom airflow. The outer airflow and the bottom airflow drive the water to rinse the cancellous bone in multiple directions, and cause the cancellous bone to float up and down in the rotating cage.

9. The method for preparing bone repair material based on bovine bone as described in claim 8, characterized in that, In preparation step 3), the bottom air pipe is horizontally spiraled and abuts against the rotating disk. The direction of the horizontal spiral of the bottom air pipe is different from the direction of rotation of the rotating disk. Multiple bottom air guide pipes are connected to the bottom air outlet. The lower end of the bottom air guide pipe is connected to the bottom air pipe and communicates with the bottom air blowing hole. The upper end of the bottom air guide pipe extends towards the bottom of the rotating cage. The bottom air guide pipe is inclined, and the inclination directions of the multiple bottom air guide pipes are different. The peripheral air tube is provided with multiple peripheral air guide tubes. The outer end of the peripheral air guide tube is connected to the peripheral air tube and communicates with the peripheral air blowing hole. The inner end of the peripheral air guide tube extends towards the outer periphery of the rotating cage. The peripheral air guide tubes are arranged at an angle, and the angles of the multiple peripheral air guide tubes are different. In the preparation step 3), multiple peripheral air holes blow out multiple peripheral airflows through peripheral air guide pipes. The multiple peripheral airflows are directed toward the outer periphery of the rotating cage, and the blowing directions of the multiple peripheral airflows are different. Multiple bottom air holes blow out multiple bottom airflows through bottom air pipes. These multiple bottom airflows are directed toward the bottom of the rotating cage and blow in different directions. The multiple peripheral airflows and multiple bottom airflows drive water to rinse the cancellous bone in multiple directions and cause the cancellous bone to float up and down in the rotating cage.

10. The method for preparing bovine bone repair material according to any one of claims 1-7, characterized in that, In preparation step 4), the cancellous bone is placed in a heating furnace, which has a furnace cavity containing water; a suspended metal mesh is provided in the furnace cavity, which has a mesh cavity with an opening at the top and multiple mesh holes. The outer periphery of the mesh cover is connected to multiple elastic sheets. The outer ends of the elastic sheets are fixedly connected to the inner sidewall of the furnace cavity, and the inner ends of the elastic sheets are connected to the outer periphery of the mesh cover. The multiple elastic sheets are arranged at intervals around the circumference of the mesh cover, and the middle part of the elastic sheets is bent downward. In preparation step 4), the cancellous bone is placed in the mesh cavity, the water body is submerged over the mesh cover and multiple elastic sheets, the water body is heated to a boiling state, the water body boils the cancellous bone, and the cancellous bone is driven up and down by the boiling water body, and the multiple elastic sheets synchronously drive the mesh cover up and down, so that the cancellous bone in the mesh cavity is in a floating state.

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