Parameter adjustment method and device of detection equipment, equipment, medium and program product

Abstract

The invention relates to a parameter adjustment method and device of detection equipment, equipment, a medium and a program product. The method comprises the following steps: in the process of carrying out biological detection on a target sample by utilizing detection equipment, controlling a laser of the detection equipment to carry out laser trial emission, obtaining a laser trial emission drop point position, a laser trial emission beam spot and sample information corresponding to the target sample, and determining the target sample according to the laser trial emission drop point position, the laser trial emission beam spot and the sample information, and determining laser adjustment parameters of the laser, and performing parameter adjustment on the laser according to the laser adjustment parameters. By adopting the method, the control efficiency of the detection equipment can be improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of automatic control, in particular to a parameter adjustment method and device of detection equipment, equipment, medium and program product. BACKGROUND

[0002] With the continuous development of biological detection technology, more and more biological detection equipment has also been produced, and biological researchers can quickly detect biological samples through biological detection equipment. Among them, the MALDI (Matrix-Assisted Laser Desorption / Ionization, MALDI) source mass spectrometry system is widely used in microbiological identification, proteomics, metabolomics and some clinical detection scenes due to its fast and accurate characteristics. MALDI is a "soft ionization" technology used to gently convert large molecules (such as proteins, polypeptides, nucleic acids, polysaccharides, etc.) from solid samples into gas phase ions without causing significant fragmentation, thereby suitable for mass spectrometry analysis of high molecular weight biomolecules.

[0003] At present, during the operation of the MALDI equipment, the adjustment of parameters such as laser angle and laser intensity is usually performed manually, which makes the control efficiency of the equipment low. SUMMARY

[0004] Therefore, it is necessary to provide a parameter adjustment method, device, equipment, medium and program product of detection equipment capable of improving the control efficiency of the detection equipment.

[0005] In a first aspect, the present application provides a parameter adjustment method of detection equipment, comprising: in the process of biological detection of a target sample by using detection equipment, controlling a laser of the detection equipment to perform a laser test shot, and acquiring a laser test shot landing point position, a laser test shot beam spot and sample information corresponding to the target sample; determining a laser adjustment parameter of the laser according to the laser test shot landing point position, the laser test shot beam spot and the sample information; and adjusting the parameter of the laser according to the laser adjustment parameter.

[0006] In one of the embodiments, the sample information includes a sample area and a sample position, and the laser adjustment parameter of the laser is determined according to the laser test shot landing point position, the laser test shot beam spot and the sample information, comprising: determining a position adjustment parameter of the laser according to the laser test shot landing point position and the sample position; determining a beam spot adjustment parameter of the laser according to the laser test shot beam spot and the sample area; and determining the laser adjustment parameter according to the position adjustment parameter and the beam spot adjustment parameter.

[0007] In one of the embodiments, the position adjustment parameter of the laser is determined according to the laser test shot position and the sample position, including: calculating the relative distance between the laser test shot position and the sample position; determining the coordinate adjustment parameter of the laser according to the relative distance, determining the position adjustment parameter according to the coordinate adjustment parameter, and the coordinate adjustment parameter includes the laser coordinate adjustment angle and / or the lens deflection adjustment angle.

[0008] In one of the embodiments, the beam spot adjustment parameter of the laser is determined according to the laser test shot beam spot and the sample area, including: calculating the area difference between the laser test shot beam spot and the sample area; determining the lens distance of the laser according to the area difference, and determining the beam spot adjustment parameter according to the lens distance.

[0009] In one of the embodiments, before the laser adjustment parameter is determined according to the position adjustment parameter and the beam spot adjustment parameter, the method further includes: obtaining the sample characteristic information of the target sample and the laser test intensity, determining the intensity adjustment parameter of the laser according to the sample characteristic information and the laser test intensity; and determining the laser adjustment parameter according to the position adjustment parameter and the beam spot adjustment parameter, including: determining the laser adjustment parameter according to the position adjustment parameter, the beam spot adjustment parameter and the intensity adjustment parameter.

[0010] In one of the embodiments, the laser test intensity is obtained by an energy sensor, and the intensity adjustment parameter of the laser is determined according to the sample characteristic information and the laser test intensity, including: determining the laser loss according to the laser test intensity and the reference laser test intensity; querying the reference laser intensity corresponding to the sample characteristic information, and determining the target laser intensity according to the reference laser intensity and the laser loss; and determining the intensity adjustment parameter according to the target laser intensity.

[0011] In a second aspect, the application further provides a parameter adjustment device of a detection equipment, including: an information acquisition module, configured to control a laser of the detection equipment to perform laser test in a biological detection process of a target sample, and acquire a laser test shot position, a laser test beam spot and sample information corresponding to the target sample; a parameter determination module, configured to determine a laser adjustment parameter of the laser according to the laser test shot position, the laser test beam spot and the sample information; and a parameter adjustment module, configured to adjust the parameter of the laser according to the laser adjustment parameter.

[0012] In a third aspect, the application further provides a computer device, including a memory and a processor, the memory stores a computer program, and the processor implements the steps of the method of the first aspect when executing the computer program.

[0013] In a fourth aspect, the application further provides a computer readable storage medium, which stores a computer program, and the computer program is executed by a processor to implement the steps of the method of the first aspect.

[0014] In a fifth aspect, the present application also provides a computer program product comprising a computer program which, when executed by a processor, implements the steps of the method of the first aspect.

[0015] The parameter adjustment method, device, equipment, medium and program product of the detection equipment, in the process of biological detection of the target sample by the detection equipment, first control the laser of the detection equipment to carry out laser test firing, through the test firing mode, the sample information corresponding to the laser test firing drop position, the laser test firing beam spot and the target sample are obtained without causing damage to the sample, the laser adjustment parameters of the laser are determined according to the laser test firing drop position, the laser test firing beam spot and the sample information, the parameter adjustment of the laser is carried out according to the laser adjustment parameters, the automatic adjustment of the laser is realized through the automatic control of the detection equipment without damaging the sample and introducing additional operation, the problem of low control efficiency caused by manual adjustment according to experience is avoided, and the control efficiency of the detection equipment is improved. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the related art, the drawings needed to be used in the description of the embodiments of the present application or the related art will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0017] Figure 1 The application environment diagram of the parameter adjustment method of the detection equipment in an embodiment;

[0018] Figure 2 The flowchart of the parameter adjustment method of the detection equipment in an embodiment;

[0019] Figure 3 The flowchart of step 202 in an embodiment;

[0020] Figure 4 The flowchart of step 301 in an embodiment;

[0021] Figure 5 The flowchart of step 302 in an embodiment;

[0022] Figure 6 The flowchart of the intensity adjustment parameter determination step in an embodiment;

[0023] Figure 7 The flowchart of step 602 in an embodiment;

[0024] Figure 8A flowchart of a parameter adjustment method of a detection device in another embodiment;

[0025] Figure 9 A block diagram of a parameter adjustment device of a detection device in an embodiment;

[0026] Figure 10 An internal structure diagram of a computer device in an embodiment. DETAILED DESCRIPTION

[0027] For the purpose, technical solutions and advantages of the present application to be clearer, further detailed description will be made to the present application in combination with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, and are not used to limit the present application.

[0028] It should be noted that the terms "first", "second" and the like used in the present application can be used to describe various elements, but these elements are not limited by these terms. These terms are only used to distinguish the first element from the second element. The terms "include" and "have" and any variations thereof used in the present application are intended to cover non-exclusive inclusion. The term "multiple" used in the present application refers to two and more than two. The term "and / or" used in the present application refers to one of the options or any combination of multiple options.

[0029] The parameter adjustment method of the detection device provided by the embodiments of the present application can be applied in an application environment as shown in Figure 1 The application environment at least includes a detection device 101 and a control device 102.

[0030] The detection device 101 is used to perform laser test firing and parameter adjustment according to the control instruction of the control device 102. The detection device 101 is a sample detection device corresponding to a MALDI source mass spectrum system, which can be a time-of-flight (TOF) mass analyzer, mainly composed of a laser, a target plate, an ion acceleration zone, a flight tube, an ion detector (such as a microchannel plate MCP), and a signal processing system. The laser irradiates the sample target plate to generate ions, the ions enter the flight tube through the acceleration zone, and after being separated according to the mass-to-charge ratio in the field-free drift region, the ions hit the detector, and the signal is collected and analyzed by the processing system.

[0031] The control device 102 is used to control the laser included in the detection device 101 to perform laser test firing, and to obtain the laser test firing landing point position, the laser test firing beam spot, and the sample information corresponding to the target sample. According to the laser test firing landing point position, the laser test firing beam spot, and the sample information corresponding to the target sample, the parameter of the laser is adjusted. The control device 102 can include a data processing unit 102-1 and an image acquisition unit 102-2. The laser test firing landing point position, the laser test firing beam spot, and the sample information corresponding to the target sample can be obtained by the image acquisition unit.

[0032] It should be noted that the control device 102 can be embedded in the detection device 101, that is, the control device 102 can be a unit module of the detection device 101; or the control device 102 can be deployed independently of the detection device 101, in which case the control device 102 can be electrically connected to the detection device 101. The image acquisition range of the image acquisition unit 102-2 can be the target plate area of the detection device 101.

[0033] In an exemplary embodiment, as shown in Figure 2 , a parameter adjustment method of a detection device is provided, which is described by taking the control device in Figure 1 as an example, including the following steps 201 to 203.

[0034] Step 201, in the process of biological detection of a target sample by using a detection device, the laser of the detection device is controlled to perform a laser test shot, and the laser test shot landing position, the laser test shot beam spot, and the sample information corresponding to the target sample are acquired.

[0035] In this application, the detection device refers to the detection device corresponding to the MALDI source mass spectrometry system, wherein MALDI is a "soft ionization" technology, which is used to gently convert large molecules (such as proteins, polypeptides, nucleic acids, polysaccharides, etc.) from solid samples into gas phase ions without causing significant fragmentation, and then used for mass spectrometric analysis of high molecular weight biological molecules. In this application, the target sample refers to the biological sample corresponding to the large molecule, which can be solid, liquid, gas, or a mixture of any two or more of the above three forms.

[0036] In the implementation process, the operation user first performs biological detection on the target sample by using the detection device, can place the target sample on the target plate of the detection device, and inputs a biological detection instruction to the detection device to start the biological detection; at this time, the control device controls the laser of the detection device to perform a laser test shot, and acquires the laser test shot landing position, the laser test shot beam spot, and the sample information corresponding to the target sample. The beam spot refers to the irradiation area formed on the target surface by the focused particle beam (such as electron beam, ion beam or laser beam), which reflects the focusing degree and spatial distribution characteristics of the beam.

[0037] In the execution process, after detecting the biological detection instruction, the control device controls the laser of the detection device to perform a laser test shot, and acquires the laser test shot landing position, the laser test shot beam spot, and the sample information corresponding to the target sample.

[0038] In the process of controlling the laser test, the test intensity can be preset to perform the laser test according to the preset test intensity, so as to avoid damage to the target sample; or the initial laser position and the initial laser beam spot can be preset, and the laser test is performed according to the initial laser position and the initial laser beam spot. In addition, the laser test can also be performed according to the historical laser position and the historical laser beam spot, such as performing the laser test according to the last laser position and the laser beam spot.

[0039] In the process of acquiring the laser test landing position, the laser test beam spot, and the sample information, the control device can acquire the image of the target plate region through the image acquisition unit, and the control device performs image recognition on the image to obtain the laser test landing position, the laser test beam spot, and the sample information. Optionally, the sample information includes sample area information and sample position information of the target sample.

[0040] In addition, the control device can also acquire the data of the target plate region through the sensing unit, and the control device can determine the sample information of the target sample through the pressure sensor, and determine the laser test landing position and the laser test beam spot through the energy sensor.

[0041] It should be noted that the sample information corresponding to the target sample can also be preset information, such as the sample position information and the sample area information can be indicated on the target plate, and the operation user can place the target sample in the area indicated by the target plate; in this case, the target sample can not be placed on the target plate.

[0042] Step 202, determining the laser adjustment parameter of the laser device according to the laser test landing position, the laser test beam spot, and the sample information.

[0043] In the implementation process, the control device can determine the position adjustment parameter of the laser device according to the laser test landing position and the sample information, and determine the laser beam spot adjustment parameter of the laser device according to the laser test beam spot and the sample information.

[0044] In the execution process, the control device can also input the laser test landing position, the laser test beam spot, and the sample information into a pre-trained model or network to obtain the laser adjustment parameter output by the model or network. Optionally, the laser adjustment parameter includes at least one of the laser position adjustment parameter, the laser beam spot adjustment parameter, and the laser energy adjustment parameter.

[0045] Step 203, parameter adjustment is performed on the laser device according to the laser adjustment parameter.

[0046] In the implementation process, the control device generates an adjustment instruction for the laser device of the detection device according to the laser adjustment parameter, and sends the adjustment instruction to the detection device; the detection device performs parameter adjustment on the laser device in response to the adjustment instruction.

[0047] In addition, in the case where the control device is embedded in the detection device as a control unit, the control device can perform parameter adjustment on the laser of the detection device based on the adjustment instruction after generating the adjustment instruction.

[0048] In the parameter adjustment method of the detection device, in the process of biological detection of the target sample by the detection device, first, the laser of the detection device is controlled to perform a laser test shot, and the laser test shot landing point position, the laser test shot beam spot, and the sample information corresponding to the target sample are obtained through the test shot without causing damage to the sample. The laser adjustment parameters of the laser are determined according to the laser test shot landing point position, the laser test shot beam spot, and the sample information. The parameter adjustment is performed on the laser according to the laser adjustment parameters, so that the automatic adjustment of the laser is realized through the automatic control of the detection device without damaging the sample and introducing additional operations. The problem of low control efficiency caused by manual adjustment according to experience is avoided, and the control efficiency of the detection device is improved.

[0049] Based on the above one exemplary embodiment, the following is provided in one or more exemplary embodiments. A parameter adjustment method of a detection device is provided, which is applied to Figure 1 the control device in the above embodiment, and specifically includes the following contents.

[0050] In the process of determining the laser adjustment parameters, the position adjustment parameters of the laser can be determined first, then the beam spot adjustment parameters of the laser are determined, and then the laser adjustment parameters are determined. In an optional embodiment provided by the present application, as shown in Figure 3 step 202 includes steps 301 to 303:

[0051] Step 301: determining the position adjustment parameters of the laser according to the laser test shot landing point position and the sample position.

[0052] In the implementation process, the control device determines the landing point offset distance of the laser according to the laser test shot landing point position and the sample position, and determines the position adjustment parameters of the laser according to the landing point offset distance.

[0053] In the execution process, the laser landing point can be determined by the three-axis coordinates of the laser or by the deflection lens. The control device can calculate the three-axis coordinate adjustment parameters of the laser as the position adjustment parameters, or the control device can calculate the deflection angle adjustment parameters of the deflection lens of the laser as the position adjustment parameters of the laser.

[0054] Step 302: determining the beam spot adjustment parameters of the laser according to the laser test shot beam spot and the sample area.

[0055] In the implementation process, the control device can determine the beam spot adjustment parameter of the laser according to the laser test shot beam spot and the sample area, the difference between the beam spot and the area, and the difference.

[0056] In step 303, the laser adjustment parameter is determined according to the position adjustment parameter and the beam spot adjustment parameter.

[0057] In the implementation process, the control device integrates the position adjustment parameter and the beam spot adjustment parameter to obtain the laser adjustment parameter.

[0058] An optional embodiment provided in the present application calculates the parameters corresponding to different structures respectively, thereby improving the accuracy and reliability of the laser adjustment parameter, and further improving the automatic control reliability of the detection device.

[0059] In the process of determining the position adjustment parameter of the laser, the control device can determine the position adjustment parameter according to the relative distance between the laser test shot landing point position and the sample position; in an optional embodiment provided in the present application, as shown in Figure 4 Step 301 includes steps 401 to 402.

[0060] In step 401, the relative distance between the laser test shot landing point position and the sample position is calculated.

[0061] In the implementation process, the control device calculates the relative distance between the laser test shot landing point position and the sample position according to the coordinates of the laser test shot landing point position and the coordinates of the sample position.

[0062] In the calculation process, the relative distance can be calculated by image processing based on the laser test shot landing point position of the laser test shot landing point in the image and the sample position of the target sample in the image.

[0063] For example, a high-definition miniature camera is installed above the sample to be tested to detect the laser shape of the laser-sample contact surface and the sample shape of the sample to be tested. The position data of the two shapes is uploaded through image collection and processing, so that the control device sets the center position, the center is upward, the center is downward, and other parameters to control the XYZ three coordinates of the laser input laser and a series of lens deflection angles, so as to ensure that the laser shooting position reaches the preset position.

[0064] In step 402, the coordinate adjustment parameter of the laser is determined according to the relative distance, and the position adjustment parameter is determined according to the coordinate adjustment parameter.

[0065] In the implementation process, the control device determines the coordinate adjustment parameter of the laser according to the relative distance, and determines the position adjustment parameter according to the coordinate adjustment parameter; wherein the coordinate adjustment parameter includes a laser coordinate adjustment angle and / or a lens deflection adjustment angle.

[0066] In the execution process, the control device can determine the three-axis coordinate adjustment angle of the laser and the deflection lens adjustment angle according to the relative distance, and determine the position adjustment parameter of the laser according to the three-axis coordinate adjustment angle and the deflection lens adjustment angle, so as to control the base three-axis and the deflection lens of the laser to adjust at the same time.

[0067] In an optional embodiment provided by the present application, the position of the laser landing point is controlled by calculating the three-axis coordinate adjustment angle of the base of the laser and the deflection lens adjustment angle, which improves the accuracy of automatic control of the detection device.

[0068] In the beam spot adjustment parameter determination process, the convex lens distance can be calculated as the beam spot adjustment parameter. In an optional embodiment provided by the present application, as shown in FIG. 5, step 302 includes steps 501 and 502. Figure 5

[0069] Step 501: Calculate the area difference between the laser test beam spot and the sample area.

[0070] In the implementation process, the control device calculates the area difference between the area corresponding to the laser test beam spot and the sample area. In the calculation process, the control device can obtain the area corresponding to the laser test beam spot and the sample area based on the image, and determine the area difference between the laser test beam spot and the sample area according to the image processing algorithm.

[0071] Step 502: Determine the lens distance of the laser according to the area difference, and determine the beam spot adjustment parameter according to the lens distance.

[0072] In the implementation process, the control device can determine the lens distance of the laser according to the area difference, which can be the adjustment distance of the convex lens of the laser, and determine the beam spot adjustment parameter according to the lens distance.

[0073] In the lens distance determination process, the advancing distance or the retreating distance of the convex lens can be determined according to the area difference, and a preset data table can be queried for determination.

[0074] For example, the camera uploads the laser shooting position light spot image data, and the beam size is compared with the preset size through graphic data processing, so that the sample end laser beam spot is the preset parameter.

[0075] In an optional embodiment provided by the present application, the lens distance of the laser is determined by calculating the area difference between the beam spot and the sample area, which accurately controls the beam spot of the laser, and further improves the reliability of the automatic control of the detection device.

[0076] ​In actual scenarios, different samples also correspond to different laser energies, because different substrates (such as CHCA, SA, DHB, etc.) have different absorption efficiencies for laser of specific wavelengths; in response to this, the control device can also control the laser intensity of the laser; in an optional embodiment provided by the present application, as shown in Figure 6 the control device can acquire sample information corresponding to the laser test drop point position, the laser test beam spot, and the target sample; after the laser test drop point position, the laser test beam spot, and the sample information are acquired, or after step 302 is executed, steps 601 to 602 are executed:

[0077] In the implementation process, the sample characteristic information of the target sample can be pre-stored in the control device, and the control device acquires the sample characteristic information of the target sample and acquires the laser test intensity.

[0078] In the implementation process, the sample characteristic information of the target sample can be pre-stored in the control device, and the control device acquires the sample characteristic information of the target sample and acquires the laser test intensity.

[0079] In the process of acquiring the sample characteristic information, the control device can acquire sample name information input by a user, and acquire the sample characteristic information according to the sample name information; in the process of acquiring the laser test intensity, the control device can acquire the laser test intensity through an energy sensor. Optionally, the laser test intensity is acquired through the energy sensor; the energy sensor is installed on one side of the target plate close to the sample.

[0080] Step 602: determining the intensity adjustment parameter of the laser according to the sample characteristic information and the laser test intensity.

[0081] In the implementation process, the control device determines the preset laser intensity according to the sample characteristic information, and determines the intensity adjustment parameter of the laser according to the preset laser intensity and the laser test intensity.

[0082] Correspondingly, step 303 can be replaced by: determining the laser adjustment parameter according to the position adjustment parameter, the beam spot adjustment parameter, and the intensity adjustment parameter.

[0083] In an optional embodiment provided by the present application, the laser intensity of the laser is controlled through the laser intensity adjustment parameter, which further increases the function of the control device, and thus improves the operation convenience of the detection device and the automatic control efficiency.

[0084] In the process of determining the intensity adjustment parameter, the reference laser intensity corresponding to different samples can be pre-stored, and the intensity adjustment parameter is determined according to the reference laser intensity; in an optional embodiment provided by the present application, as shown in Figure 7 step 602 includes steps 701 to 703:

[0085] Step 701: determining the laser loss according to the laser test intensity and the reference laser test intensity.

[0086] In actual scenarios, the refraction and reflection of the laser through multiple lenses can cause path loss, which can cause sample loss for intensity-sensitive samples. In this regard, the loss of the laser can be calculated first.

[0087] In the implementation process, the laser intensity of the test laser can be pre-set, and the control device determines the laser loss according to the collected laser test intensity and the pre-set laser test intensity. The laser loss can be in the form of percentage data, such as a decrease of x% in intensity, or in the form of intensity numerical data, such as a decrease of x watts per square centimeter (W / cm²) or joules per square centimeter (J / cm²).

[0088] In step 702, the reference laser intensity corresponding to the sample characteristic information is queried, and the target laser intensity is determined according to the reference laser intensity and the laser loss.

[0089] In the implementation process, the control device queries the reference laser intensity corresponding to the sample characteristic information in the data table, adjusts the reference laser intensity according to the laser loss, and obtains the target laser intensity. The reference laser intensity can be adjusted upward by the laser loss to obtain the target laser intensity.

[0090] In step 703, the intensity adjustment parameter is determined according to the target laser intensity.

[0091] In the implementation process, the control device determines the intensity adjustment parameter according to the target laser intensity. In the determination process, the control device can generate a laser up-regulation parameter or a laser down-regulation parameter according to the target laser intensity.

[0092] For example, an energy sensor is arranged on the target plate, and the energy data is uploaded. The control device client obtains the laser energy value and distribution. If there is a deviation from the preset energy value, the laser output energy value can be automatically adjusted to the preset energy value at the sample end through the energy attenuation on the laser transmission path.

[0093] An optional embodiment provided by the present application compensates for the pre-set reference laser intensity by calculating the laser loss, avoids the way of determining the laser intensity by the operation user according to experience, improves the automatic control efficiency, and improves the reliability and stability of the automatic control.

[0094] In one embodiment, referring to Figure 8 , a flowchart of a parameter adjustment method of a detection device is shown, which can be applied to Figure 1 , the control device. As shown in Figure 8 , the parameter adjustment method of the detection device can include the following steps:

[0095] In the biological detection of the target sample by the detection device, the laser of the detection device is controlled to perform a laser test shot in step 801.

[0096] In step 802, a target plate image is captured, and the target plate image is identified to obtain a laser test shot landing point position, a laser test shot beam spot, and sample position information and sample area information corresponding to the target sample.

[0097] In step 803, the laser test shot intensity collected by the energy sensor is obtained, and sample characteristic information of the target sample is obtained.

[0098] In step 804, the relative distance between the laser test shot landing point position and the sample position is calculated, the coordinate adjustment parameter of the laser is determined according to the relative distance, and the position adjustment parameter is determined according to the coordinate adjustment parameter.

[0099] Optionally, the coordinate adjustment parameter includes a laser coordinate adjustment angle and / or a lens deflection adjustment angle.

[0100] In step 805, the area difference between the laser test shot beam spot and the sample area is calculated, the lens distance of the laser is determined according to the area difference, and the beam spot adjustment parameter is determined according to the lens distance.

[0101] In step 806, the laser loss is determined according to the laser test shot intensity and the reference laser test shot intensity.

[0102] In step 807, the reference laser intensity corresponding to the sample characteristic information is queried, the target laser intensity is determined according to the reference laser intensity and the laser loss, and the intensity adjustment parameter is determined according to the target laser intensity.

[0103] In step 808, the laser adjustment parameter is determined according to the position adjustment parameter, the beam spot adjustment parameter, and the intensity adjustment parameter.

[0104] In step 809, the laser adjustment parameter is used to adjust the laser.

[0105] It should be noted that any one step or combination of any multiple steps of steps 801 to 809 can be selected as any one step or combination of any multiple steps of steps 201 to 203 provided in the above embodiments to form a new implementation manner according to the needs of implementation deployment; and any one or more technical features in the technical solution formed by steps 801 to 809 can be selected to form a new implementation manner by combining any one or more technical features in the technical solution formed by steps 801 to 809 or by selecting the technical features in one or more optional implementation manners provided in one or more embodiments, according to the needs of actual deployment, and this will not be described here.

[0106] It should be understood that although the steps in the flowcharts involved in the embodiments described above are shown in sequence according to the arrows, the steps are not necessarily executed in the order indicated by the arrows. Unless otherwise specified herein, the execution of the steps is not strictly limited in sequence, and the steps can be executed in other sequences. Moreover, at least some of the steps in the flowcharts involved in the embodiments described above can include multiple steps or multiple stages, which are not necessarily executed at the same time, but can be executed at different times, and the execution sequence of the steps or stages is not necessarily sequential, but can be executed alternately or alternately with at least part of other steps or steps or stages in other steps. It can be understood that the steps in different embodiments can be freely combined as needed, and various non-contradictory schemes formed by the combination are within the scope of protection of the present application.

[0107] Based on the same inventive concept, the embodiments of the present application also provide a parameter adjustment device of a detection device for implementing the parameter adjustment method of the detection device. The implementation scheme for solving the problem provided by the device is similar to the implementation scheme described in the above method, so the specific limitations in one or more parameter adjustment device embodiments provided below can refer to the limitations of the parameter adjustment method of the detection device described above, which will not be repeated here.

[0108] In one exemplary embodiment, as shown in Figure 9 A parameter adjustment device of a detection device is provided, including: an information acquisition module 901, a parameter determination module 902, and a parameter adjustment module 903, wherein: the information acquisition module 901 is configured to control a laser of the detection device to perform a laser test shot during biological detection of a target sample by the detection device, and acquire a laser test shot landing position, a laser test shot beam spot, and sample information corresponding to the target sample; the parameter determination module 902 is configured to determine a laser adjustment parameter of the laser according to the laser test shot landing position, the laser test shot beam spot, and the sample information; and the parameter adjustment module 903 is configured to perform parameter adjustment on the laser according to the laser adjustment parameter.

[0109] In one embodiment, the parameter determination module 902 includes a position adjustment parameter determination unit, a beam spot adjustment parameter determination unit, and a laser adjustment parameter determination unit, wherein: the position adjustment parameter determination unit is configured to determine a position adjustment parameter of the laser according to the laser test shot landing position and a sample position; the beam spot adjustment parameter determination unit is configured to determine a beam spot adjustment parameter of the laser according to the laser test shot beam spot and a sample area; and the laser adjustment parameter determination unit is configured to determine the laser adjustment parameter according to the position adjustment parameter and the beam spot adjustment parameter.

[0110] In one of the embodiments, the position adjustment parameter determination unit comprises a distance determination unit and a parameter determination unit, wherein the distance determination unit is configured to calculate the relative distance between the laser test shot position and the sample position; and the parameter determination unit is configured to determine the coordinate adjustment parameter of the laser according to the relative distance, to determine the position adjustment parameter according to the coordinate adjustment parameter, and the coordinate adjustment parameter comprises a laser coordinate adjustment angle and / or a lens deflection adjustment angle.

[0111] In one of the embodiments, the beam spot adjustment parameter determination unit comprises an area determination unit and a parameter determination unit, wherein the area determination unit is configured to calculate the area difference between the laser test shot beam spot and the sample area; and the parameter determination unit is configured to determine the lens distance of the laser according to the area difference, and to determine the beam spot adjustment parameter according to the lens distance.

[0112] In one of the embodiments, the device further comprises a laser test shot intensity determination module configured to obtain the sample characteristic information of the target sample and the laser test shot intensity, and to determine the intensity adjustment parameter of the laser according to the sample characteristic information and the laser test shot intensity.

[0113] In one of the embodiments, the laser adjustment parameter determination unit is further configured to determine the laser adjustment parameter according to the position adjustment parameter, the beam spot adjustment parameter and the intensity adjustment parameter.

[0114] In one of the embodiments, the laser test shot intensity determination module comprises a laser loss determination unit, a laser intensity determination unit and an intensity adjustment parameter determination unit, wherein the laser loss determination unit is configured to determine the laser loss according to the laser test shot intensity and the reference laser test shot intensity; the laser intensity determination unit is configured to query the reference laser intensity corresponding to the sample characteristic information, and to determine the target laser intensity according to the reference laser intensity and the laser loss; and the intensity adjustment parameter determination unit is configured to determine the intensity adjustment parameter according to the target laser intensity.

[0115] The above-mentioned modules in the parameter adjustment device of the detection equipment can be realized by software, hardware and combinations thereof in whole or in part. The above-mentioned modules can be embedded in or independent of the processor in the computer equipment in hardware form, or can be stored in the memory in the computer equipment in software form, so as to be called and executed by the processor to perform the operations corresponding to the above-mentioned modules.

[0116] In one of the embodiments, a computer equipment is provided, which can be a server, and the internal structure diagram thereof can be as shown in Figure 10The computer device includes a processor, a memory, an input / output interface (I / O), and a communication interface. The processor, the memory, and the input / output interface are connected through a system bus, and the communication interface is connected to the system bus through the input / output interface. The processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program, and a database. The internal memory provides an environment for running the operating system and the computer program in the non-volatile storage medium. The database of the computer device is configured to store parameter adjustment data of the detection device. The input / output interface of the computer device is configured to exchange information between the processor and external devices. The communication interface of the computer device is configured to communicate with external terminals through a network connection. The computer program is executed by the processor to implement a parameter adjustment method of a detection device.

[0117] Those skilled in the art can understand that, Figure 10 The structure shown in the figure is only a block diagram of part of the structure related to the scheme of the present application, and does not constitute a limitation on the computer device to which the scheme of the present application is applied. A specific computer device can include more or fewer components than those shown in the figure, or combine certain components, or have a different arrangement of components.

[0118] In one exemplary embodiment, a computer device is provided, including a memory and a processor, the memory storing a computer program, and the processor executing the computer program to implement the following steps: in the process of biological detection of a target sample by using a detection device, controlling a laser of the detection device to perform a laser test shot, and acquiring a laser test shot landing position, a laser test shot beam spot, and sample information corresponding to the target sample; determining laser adjustment parameters of the laser according to the laser test shot landing position, the laser test shot beam spot, and the sample information; and adjusting parameters of the laser according to the laser adjustment parameters.

[0119] In one embodiment, the processor executing the computer program further implements the following steps: determining a position adjustment parameter of the laser according to the laser test shot landing position and the sample position; determining a beam spot adjustment parameter of the laser according to the laser test shot beam spot and the sample area; and determining the laser adjustment parameters according to the position adjustment parameter and the beam spot adjustment parameter.

[0120] In one embodiment, the processor executing the computer program further implements the following steps: calculating a relative distance between the laser test shot landing position and the sample position; determining a coordinate adjustment parameter of the laser according to the relative distance, and determining the position adjustment parameter according to the coordinate adjustment parameter, the coordinate adjustment parameter including a laser coordinate adjustment angle and / or a lens deflection adjustment angle.

[0121] In one embodiment, the processor, when executing the computer program, further implements the following steps: calculating an area difference between the laser test beam spot and the sample area; determining a lens distance of the laser according to the area difference, and determining the beam spot adjustment parameter according to the lens distance.

[0122] In one embodiment, the processor, when executing the computer program, further implements the following steps: obtaining sample characteristic information of the target sample and a laser test intensity, and determining an intensity adjustment parameter of the laser according to the sample characteristic information and the laser test intensity.

[0123] In one embodiment, the processor, when executing the computer program, further implements the following steps: determining the laser adjustment parameter according to the position adjustment parameter, the beam spot adjustment parameter and the intensity adjustment parameter.

[0124] In one embodiment, the processor, when executing the computer program, further implements the following steps: determining a laser loss according to the laser test intensity and a reference laser test intensity; querying a reference laser intensity corresponding to the sample characteristic information, and determining a target laser intensity according to the reference laser intensity and the laser loss; and determining the intensity adjustment parameter according to the target laser intensity.

[0125] In one embodiment, a computer readable storage medium is provided, and the computer readable storage medium stores a computer program. The computer program, when executed by a processor, implements the following steps: in a process of biological detection of a target sample by a detection device, controlling a laser of the detection device to perform laser test, and obtaining a laser test landing point position, a laser test beam spot and sample information corresponding to the target sample; determining a laser adjustment parameter of the laser according to the laser test landing point position, the laser test beam spot and the sample information; and adjusting the laser according to the laser adjustment parameter.

[0126] In one embodiment, the processor, when executing the computer program, further implements the following steps: determining a position adjustment parameter of the laser according to the laser test landing point position and the sample position; determining a beam spot adjustment parameter of the laser according to the laser test beam spot and the sample area; and determining the laser adjustment parameter according to the position adjustment parameter and the beam spot adjustment parameter.

[0127] In one embodiment, the processor, when executing the computer program, further implements the following steps: calculating a relative distance between the laser test landing point position and the sample position; determining a coordinate adjustment parameter of the laser according to the relative distance, and determining the position adjustment parameter according to the coordinate adjustment parameter, the coordinate adjustment parameter including a laser coordinate adjustment angle and / or a lens deflection adjustment angle.

[0128] In one embodiment, the processor, when executing the computer program, further implements the following steps: calculating an area difference between the laser test beam spot and the sample area; determining a lens distance of the laser according to the area difference, and determining the beam spot adjustment parameter according to the lens distance.

[0129] In one embodiment, the processor, when executing the computer program, further implements the following steps: obtaining sample characteristic information of the target sample and the laser test intensity, and determining the intensity adjustment parameter of the laser according to the sample characteristic information and the laser test intensity.

[0130] In one embodiment, the processor, when executing the computer program, further implements the following steps: determining the laser adjustment parameter according to the position adjustment parameter, the beam spot adjustment parameter and the intensity adjustment parameter.

[0131] In one embodiment, the processor, when executing the computer program, further implements the following steps: determining the laser loss according to the laser test intensity and the reference laser test intensity, querying the reference laser intensity corresponding to the sample characteristic information, determining the target laser intensity according to the reference laser intensity and the laser loss, and determining the intensity adjustment parameter according to the target laser intensity.

[0132] In one embodiment, a computer program product is provided, comprising a computer program which, when executed by a processor, implements the following steps: in the process of biological detection of a target sample by a detection device, controlling a laser of the detection device to perform a laser test, and obtaining a laser test landing point position, a laser test beam spot and sample information corresponding to the target sample; determining a laser adjustment parameter of the laser according to the laser test landing point position, the laser test beam spot and the sample information; and adjusting the laser according to the laser adjustment parameter.

[0133] In one embodiment, the processor, when executing the computer program, further implements the following steps: determining the position adjustment parameter of the laser according to the laser test landing point position and the sample position, determining the beam spot adjustment parameter of the laser according to the laser test beam spot and the sample area, and determining the laser adjustment parameter according to the position adjustment parameter and the beam spot adjustment parameter.

[0134] In one embodiment, the processor, when executing the computer program, further implements the following steps: calculating the relative distance between the laser test landing point position and the sample position, determining the coordinate adjustment parameter of the laser according to the relative distance, determining the position adjustment parameter according to the coordinate adjustment parameter, and the coordinate adjustment parameter comprising a laser coordinate adjustment angle and / or a lens deflection adjustment angle.

[0135] In one embodiment, the processor, when executing the computer program, further implements the following steps: calculating the area difference between the laser test beam spot and the sample area, determining the lens distance of the laser according to the area difference, and determining the beam spot adjustment parameter according to the lens distance.

[0136] In one embodiment, the processor, when executing the computer program, further implements the following steps: obtaining sample characteristic information of the target sample and the laser test intensity, and determining the intensity adjustment parameter of the laser according to the sample characteristic information and the laser test intensity.

[0137] In one embodiment, the processor, when executing the computer program, also implements the following steps: determining the laser adjustment parameter according to the position adjustment parameter, the beam spot adjustment parameter and the intensity adjustment parameter.

[0138] In one embodiment, the processor, when executing the computer program, also implements the following steps: determining the laser loss according to the laser test intensity and the reference laser test intensity; querying the reference laser intensity corresponding to the sample feature information, and determining the target laser intensity according to the reference laser intensity and the laser loss; and determining the intensity adjustment parameter according to the target laser intensity.

[0139] It should be noted that the data (including but not limited to data for analysis, stored data, displayed data, etc.) involved in the present application are all information and data authorized by the user or fully authorized by all parties, and the collection, use and processing of related data need to comply with relevant regulations.

[0140] Those skilled in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be completed by instructing the relevant hardware through a computer program. The computer program can be stored in a non-volatile computer readable storage medium, and when executed, can include the processes of the above-mentioned embodiment methods. Any reference to memory, database or other medium used in the embodiments provided in the present application can include at least one of non-volatile memory and volatile memory. The non-volatile memory can include read-only memory (ROM), magnetic tape, floppy disk, flash memory, optical storage, high-density embedded non-volatile memory, resistive random access memory (ReRAM), magnetoresistive random access memory (MRAM), ferroelectric random access memory (FRAM), phase change memory (PCM), graphene memory, etc. The volatile memory can include random access memory (RAM) or external cache memory, etc. As an illustration but not limitation, the RAM can be in various forms, such as static random access memory (SRAM) or dynamic random access memory (DRAM), etc. The database involved in the embodiments provided in the present application can include at least one of a relational database and a non-relational database. The non-relational database can include a distributed database based on a block chain, etc., without being limited thereto. The processor involved in the embodiments provided in the present application can be a general-purpose processor, a central processing unit, a graphics processing unit, a digital signal processor, a programmable logic device, a data processing logic device based on quantum computing, an artificial intelligence (AI) processor, etc., without being limited thereto.

[0141] The technical features of the above embodiments can be combined in any manner. To make the description concise, not all possible combinations of the technical features in the above embodiments are described, but as long as the combinations of the technical features do not exist contradictions, they should be considered as the scope of the present application.

[0142] The above-described embodiments are merely illustrative of several embodiments of the present application, and the description is relatively specific and detailed, but should not be understood as a limitation on the scope of the patent. It should be noted that for those skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the scope of the present application. Therefore, the scope of protection of the present application should be subject to the appended claims.

Claims

1. A parameter adjustment method of a detection device, characterized by, The method comprises: In the process of biological detection of a target sample by a detection device, a laser test is performed on the laser of the detection device, and a laser test landing point position, a laser test beam spot and sample information corresponding to the target sample are obtained; According to the laser test landing point position, the laser test beam spot and the sample information, laser adjustment parameters of the laser are determined; According to the laser adjustment parameters, the laser is adjusted.

2. The method of adjusting parameters of a detection device according to claim 1, wherein, The sample information includes sample area and sample position, and the laser adjustment parameters of the laser are determined according to the laser test landing point position, the laser test beam spot and the sample information, comprising: According to the laser test landing point position and the sample position, the position adjustment parameters of the laser are determined; According to the laser test beam spot and the sample area, the beam spot adjustment parameters of the laser are determined; According to the position adjustment parameters and the beam spot adjustment parameters, the laser adjustment parameters are determined.

3. The method of adjusting parameters of a detection device according to claim 2, wherein, According to the laser test landing point position and the sample position, the position adjustment parameters of the laser are determined, comprising: The relative distance between the laser test landing point position and the sample position is calculated; According to the relative distance, the coordinate adjustment parameters of the laser are determined, and the position adjustment parameters are determined according to the coordinate adjustment parameters, the coordinate adjustment parameters including laser coordinate adjustment angle and / or lens deflection adjustment angle.

4. The method of adjusting parameters of a detection device according to claim 2, wherein According to the laser test beam spot and the sample area, the beam spot adjustment parameters of the laser are determined, comprising: The area difference between the laser test beam spot and the sample area is calculated; According to the area difference, the lens distance of the laser is determined, and the beam spot adjustment parameters are determined according to the lens distance.

5. The parameter adjustment method of a detection apparatus according to any one of claims 2 to 4, characterized by, Before the laser adjustment parameters are determined according to the position adjustment parameters and the beam spot adjustment parameters, the sample feature information and the laser test intensity of the target sample are obtained, and the intensity adjustment parameters of the laser are determined according to the sample feature information and the laser test intensity. According to the position adjustment parameters, the beam spot adjustment parameters and the intensity adjustment parameters, the laser adjustment parameters are determined. The laser test intensity is obtained by an energy sensor, and the intensity adjustment parameters of the laser are determined according to the sample feature information and the laser test intensity, comprising: According to the laser test intensity and the reference laser test intensity, the laser loss is determined; 6. The method of adjusting parameters of a detection device according to claim 5, wherein The reference laser intensity corresponding to the sample feature information is queried, and the target laser intensity is determined according to the reference laser intensity and the laser loss; The intensity adjustment parameters are determined according to the target laser intensity. The device comprises: An information acquisition module is configured to control a laser of a detection device to perform a laser test in the process of biological detection of a target sample by the detection device, and to obtain a laser test landing point position, a laser test beam spot and sample information corresponding to the target sample; 7. A parameter adjustment apparatus of a detection device, characterized by comprising: ​ ​ A parameter determination module is configured to determine laser adjustment parameters of the laser according to the laser test shot landing point position, the laser test shot beam spot and the sample information. A parameter adjustment module is configured to adjust parameters of the laser according to the laser adjustment parameters.

8. A computer device comprising a memory and a processor, the memory storing a computer program, characterized in that, The computer program is executed by the processor to implement the steps of the parameter adjustment method of the detection device according to any one of claims 1 to 6.

9. A computer readable storage medium having stored thereon a computer program, characterized in that, The computer program is executed by the processor to implement the steps of the parameter adjustment method of the detection device according to any one of claims 1 to 6.

10. A computer program product comprising a computer program, characterized in that, The computer program is executed by the processor to implement the steps of the parameter adjustment method of the detection device according to any one of claims 1 to 6.

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