Burn and scald repair gel and preparation method thereof

Abstract

The invention relates to burn and scald repair gel and a preparation method thereof.The burn and scald repair gel is composed of amorpha fruticosa extract, acrylic resin, an antioxidant and a penetrant, the amorpha fruticosa extract serving as an effective component has a good treatment effect on skin burns and scalds, and the prepared burn and scald repair gel can promote wound healing and reduce swelling and pain, and can be used for treating skin burns and scalds. The preparation can promote epidermis recovery and reduce scars, and is a green, safe and non-irritant burn and scald repair preparation.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of burn prevention and treatment, and particularly relates to a burn repair gel and a preparation method thereof. BACKGROUND

[0002] Burns refer to injuries caused by boiling water (oil), fire, electricity, light or chemicals acting on the skin or mucous membrane, which can cause local skin redness, swelling, pain, blisters, damage, etc., and can even injure the subcutaneous tissues such as muscles, bones, joints and internal organs, and is one of the highest incidence of injuries in daily work and life. As a common traumatic disease in clinical surgery, most burns are small and medium area burns of degree II or below, which can cause great physical and mental pain and harm to patients after occurrence.

[0003] At present, there is no specific drug and treatment for burns in clinical practice. Western medicine treatment mainly focuses on controlling wound infection, and drugs such as antibacterial drugs and silver salts can play a role in anti-inflammatory, antibacterial and analgesic. Western medicine treatment has a long healing time and is difficult to promote burn wound healing and inhibit the proliferation of pathological scars; Chinese medicine treatment of burns mainly uses internal and external application of compound traditional Chinese medicine. Although the traditional Chinese medicine formula is relatively mature and widely used, its treatment effect is uneven, the combination and formula proportion of medicinal materials are different, and there are problems such as large dosage, unclear material basis, dose-effect relationship and action mechanism. Therefore, the invention of a good burn repair preparation can not only alleviate the pain of patients, effectively reduce further damage, but also reduce scars, speed up wound recovery, shorten the treatment period, save treatment costs, and even save patients' lives.

[0004] Amorpha fruticosa is a perennial deciduous shrub of Leguminosae, also known as cotton wisteria, cotton strip and wisteria. Amorpha fruticosa is rich in polysaccharides, flavonoids, stilbene glycosides, triterpenes, polyphenols and other bioactive components, and has good medicinal value. The document "China Pharmaceutical University. Chinese Medicine Dictionary. Volume III. First Edition. Beijing. China Medical Science and Technology Press, 1997:861" discloses that the plant of Amorpha fruticosa has the effects of removing dampness and swelling, and is used for treating carbuncle, eczema and burns. The application of Amorpha fruticosa is a single formula, and there is no research and description of compound, nor detailed description of the preparation of Amorpha fruticosa extract. There are problems such as complicated decoction, inconvenient application, unstable effect, etc. in the application of traditional Chinese medicine prescription. It is of great significance to develop a green, safe, non-irritating, convenient and fast burn gel preparation using Amorpha fruticosa extract as the effective component for promoting wound healing after burns, reducing redness and pain, promoting epidermal recovery, reducing scar formation and developing the function of Amorpha fruticosa for burns. SUMMARY

[0005] This invention discloses a burn repair gel and its preparation method, aiming to solve the problems existing in current burn treatment methods and in the preparation of burn preparations using Amorpha fruticosa. It provides a green, safe, non-irritating repair preparation that reduces scarring for burn treatment and offers an effective approach for the development of Amorpha fruticosa burn preparations.

[0006] The first objective of this invention is to provide a burn repair gel, the components and their mass percentages of which are: 70%~95% Amorpha fruticosa extract, 0.5%~5.0% acrylic resin, 2%~15% antioxidant, and 1%~15% penetrant.

[0007] The second objective of this invention is to provide a method for preparing the above-mentioned Amorpha fruticosa extract, the specific steps of which are as follows: (1) Fresh Amorpha fruticosa stems and leaves and 80% ethanol solution were weighed at a weight ratio of 1:8. The Amorpha fruticosa stems and leaves were then ground by a colloid mill. During the grinding process, 80% ethanol solution was added to obtain a homogenized Amorpha fruticosa slurry. (2) The homogenate of Amorpha fruticosa was ultrasonically treated at 15℃~35℃ for 20~60 min and then filtered through a 0.22μm filter membrane to obtain filtrate 1 and filter residue 1; (3) The filter residue and sterile water are added and mixed at a weight ratio of 1:5. After ultrasonic treatment at 10℃~30℃ for 20~60 min, the mixture is filtered through a 0.22μm filter membrane to obtain filtrate 2 and filter residue 2. (4) After combining filtrate 1 and filtrate 2, the solution was concentrated under reduced pressure at -0.1 MPa and 40℃~50℃ for 30~50 min to obtain Amorpha fruticosa extract.

[0008] Furthermore, the antioxidant is one or more of vitamin C, tea polyphenols, vitamin E, propyl gallate, and ascorbic acid stearate; Furthermore, the penetrant is one or a mixture of propylene glycol, azone, or both.

[0009] The advantages of this invention are: the effective ingredient in the burn repair gel disclosed herein is *Amorpha fruticosa* extract, which, when combined with acrylic resin, antioxidants, and penetrants to form a repair gel, exhibits a synergistic effect, significantly improving the treatment of burns. This gel provides an organic whole for the repair and treatment of burns, producing burn treatment effects that could not be predicted by the application of *Amorpha fruticosa* extract alone. Implementation

[0010] The principles and features of the present invention are described below with reference to examples. The examples are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0011] Example 1: A burn repair gel, the composition of which is as follows (by weight percentage): The ingredients include 70% Amorpha fruticosa extract, 5.0% acrylic resin, 10% tea polyphenols, and 15% propylene glycol. The preparation method of Amorpha fruticosa extract is as follows: (1) Fresh Amorpha fruticosa stems and leaves and 80% ethanol solution were weighed at a weight ratio of 1:8. The Amorpha fruticosa stems and leaves were then ground by a colloid mill. During the grinding process, 80% ethanol solution was added to obtain a homogenized Amorpha fruticosa slurry. (2) The homogenate of Amorpha fruticosa was ultrasonically treated at 15℃ for 60 min and then filtered through a 0.22μm filter membrane to obtain filtrate 1 and filter residue 1; (3) The filter residue and sterile water were added and mixed at a weight ratio of 1:5, ultrasonically treated at 10℃ for 60 min, and then filtered through a 0.22μm filter membrane to obtain filtrate 2 and filter residue 2. (4) After combining filtrate 1 and filtrate 2, the solution was concentrated under reduced pressure at -0.1 MPa and 40℃ for 50 min to obtain Amorpha fruticosa extract.

[0012] Example 2 A burn repair gel, the composition of which is as follows (by weight percentage): The formula contains 95% Amorpha fruticosa extract, 2.0% acrylic resin, 2% vitamin C, and 1% propylene glycol. The preparation method of Amorpha fruticosa extract is as follows: (1) Fresh Amorpha fruticosa stems and leaves and 80% ethanol solution were weighed at a weight ratio of 1:8. The Amorpha fruticosa stems and leaves were then ground by a colloid mill. During the grinding process, 80% ethanol solution was added to obtain a homogenized Amorpha fruticosa slurry. (2) The homogenate of Amorpha fruticosa was ultrasonically treated at 35℃ for 20 min and then filtered through a 0.22μm filter membrane to obtain filtrate 1 and filter residue 1; (3) The filter residue and sterile water were added and mixed at a weight ratio of 1:5. After ultrasonic treatment at 30℃ for 320 min, the mixture was filtered through a 0.22μm filter membrane to obtain filtrate 2 and filter residue 2. (4) After combining filtrate 1 and filtrate 2, the solution was concentrated under reduced pressure at -0.1 MPa and 50℃ for 30 min to obtain Amorpha fruticosa extract.

[0013] Example 3 A burn repair gel, the composition of which is as follows (by weight percentage): The formula contains 80% Amorpha fruticosa extract, 0.5% acrylic resin, 15% vitamin C, and 4.5% azone. The preparation method of Amorpha fruticosa extract is as follows: (1) Fresh Amorpha fruticosa stems and leaves and 80% ethanol solution were weighed at a weight ratio of 1:8. The Amorpha fruticosa stems and leaves were then ground by a colloid mill. During the grinding process, 80% ethanol solution was added to obtain a homogenized Amorpha fruticosa slurry. (2) The homogenate of Amorpha fruticosa was ultrasonically treated at 25℃ for 40 min and then filtered through a 0.22μm filter membrane to obtain filtrate 1 and filter residue 1; (3) The filter residue and sterile water were added and mixed at a weight ratio of 1:5. After ultrasonic treatment at 20℃ for 40 min, the mixture was filtered through a 0.22μm filter membrane to obtain filtrate 2 and filter residue 2. (4) After combining filtrate 1 and filtrate 2, the solution was concentrated under reduced pressure at -0.1 MPa and 45℃ for 40 min to obtain Amorpha fruticosa extract.

[0014] Referring to Example 1, an Amorpha fruticosa extract was replaced with an equal amount of sterile water to prepare a repair gel without Amorpha fruticosa extract; The remaining technical features are the same as in Example 1.

[0015] Referring to Example 2, an acrylic resin-free repair gel was prepared by replacing the acrylic resin with an equal amount of sterile water. The remaining technical features are the same as in Example 2.

[0016] Referring to Example 3, vitamin C was replaced with an equal amount of sterile water to prepare an antioxidant-free repair gel; The remaining technical features are the same as in Example 3.

[0017] Referring to Example 2, propylene glycol was replaced with an equal amount of sterile water to prepare a repair gel without penetrant; The remaining technical features are the same as in Example 2.

[0018] Twenty-four male SD rats, each weighing 200-250 g, were purchased and, after 7 days of acclimatization, had their hair removed from both sides of the spine on their backs, creating an area of ​​approximately 6 cm × 8 cm to serve as the modeling area for burns. A 50 g weight, heated in a boiling water bath for 10 minutes, was placed against the skin of the anesthetized rat modeling area for 10 seconds using tweezers, creating two burn surfaces on each rat, for a total of 48 burn surfaces. All burn surfaces were pathologically confirmed as deep second-degree burns. One hour after the burns, the 24 rats were randomly divided into 8 groups of 3 rats each: a control group, Example 1-3 groups, and Comparative Example 1-4 groups. Each group of rats received the burn repair gel prepared in their respective group, while the control group received no repair treatment. The medication was administered twice daily, at 8:00 AM and 8:00 PM, for 21 consecutive days.

[0019] The wound area of ​​each rat was recorded on days 0, 3, 7, 14, and 21 after drug administration. The wound healing rate was calculated as follows: Wound healing rate (%) = (Initial wound area - Wound area at each time point) / Initial wound area × 100%. The results are shown in the table below.

[0020] The results in the table above show that the burn repair gel prepared in this invention can effectively promote wound healing. At 21 days, the healing rates of Examples 1-3 were significantly higher than those of the control group, and also significantly different from those of Comparative Examples 1-4. This demonstrates that the components in the burn repair gel of this invention have a synergistic effect, and are not simply a mixture of several single substances. The burn prevention and treatment ointment prepared by this mixture achieves unexpected effects that individual substances cannot provide. It is not a simple superposition of several substances and their functions, but rather an organic combination of several substances.

[0021] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A burn repair gel, characterized in that, It is a mixture of the following components by weight: 70%~95% Amorpha fruticosa extract, 0.5%~5.0% acrylic resin, 2%~15% antioxidant, and 1%~15% penetrant.

2. The burn repair gel according to claim 1, characterized in that, The preparation method of the Amorpha fruticosa extract is as follows: (1) Fresh Amorpha fruticosa stems and leaves and 80% ethanol solution were weighed at a weight ratio of 1:

8. The Amorpha fruticosa stems and leaves were then ground by a colloid mill. During the grinding process, 80% ethanol solution was added to obtain a homogenized Amorpha fruticosa slurry. (2) The homogenate of Amorpha fruticosa was ultrasonically treated at 15℃~35℃ for 20~60 min and then filtered through a 0.22μm filter membrane to obtain filtrate 1 and filter residue 1; (3) The filter residue and sterile water are added and mixed at a weight ratio of 1:

5. After ultrasonic treatment at 10℃~30℃ for 20~60 min, the mixture is filtered through a 0.22μm filter membrane to obtain filtrate 2 and filter residue 2. (4) After combining filtrate 1 and filtrate 2, the solution was concentrated under reduced pressure at -0.1 MPa and 40℃~50℃ for 30~50 min to obtain Amorpha fruticosa extract.

3. The burn repair gel according to claim 1, characterized in that, The antioxidant is one or more of vitamin C, tea polyphenols, vitamin E, propyl gallate, and ascorbic acid stearate, or a mixture thereof.

4. The burn repair gel according to claim 1, characterized in that, The penetrant is one of propylene glycol and azone, or a mixture of both.

Images