Crocodile blood composition for improving immunity and application

Abstract

The invention discloses a crocodile blood composition for improving immunity and application. The composition provided by the invention comprises the following raw materials in parts by weight: 30-60 parts of crocodile plasma powder, 1-7 parts of liquorice, 5-10 parts of radix asparagi, 3-10 parts of schisandra chinensis, 4-10 parts of codonopsis pilosula, 8-15 parts of lucid ganoderma, 2-8 parts of American ginseng and 1-5 parts of vitamin C. The composition is rich in various active substances capable of improving immunity, and can improve the immunity of the crocodile blood, promote the release level of immunomodulatory factors TNF-alpha and IL-2, increase the total number of spleen and lymphocytes and the volume of white marrow and lymph node cortex of the spleen through the cooperation of specific Chinese herbal medicine components and bioactive components in the crocodile blood, and improve the immunity of the crocodile blood. The immunosuppression effect is reduced, and the lymphocyte proliferation capacity is remarkably improved. The composition provided by the invention is green and safe in raw materials, rich in nutrition, and capable of enhancing the effect of crocodile blood and enhancing the immunity of the organism.

Description

Technical Field

[0001] This invention relates to the fields of biomedicine and food health technology, and more specifically, to a crocodile blood composition for enhancing immunity and its application. Background Technology

[0002] The immune system is a vital defense mechanism of the body, responsible for recognizing and eliminating foreign pathogens and monitoring and clearing abnormal cells. However, the human immune system can be weakened by external and internal factors, such as malnutrition, deficiencies in key trace elements, mental health issues, and unhealthy lifestyle habits. When the human immune system malfunctions, immunosuppressants such as tacrolimus (FK506), cyclosporine, rapamycin, and imidazolidinedione are often used to regulate immune function. However, these immunosuppressants have certain toxic side effects.

[0003] Existing research on the composition and efficacy of crocodile blood has revealed that it is rich in amino acids and bioactive nitrogenous compounds, offering a wide range of beneficial effects, including antibacterial, antiviral, antioxidant, anti-inflammatory, antitumor, blood pressure-lowering, cardiovascular protection, and wound-healing promotion. Furthermore, studies on Siamese crocodile blood meal have shown that crocodile blood enhances immunity; compounds such as hypoxanthine, inosine, betaine, and adenosine in crocodile blood can regulate oxidative stress, systemic regulation, and immune responses.

[0004] Currently, research on using crocodile blood for immune regulation is limited, and products containing crocodile blood to enhance immunity are also very limited. Since the immune system includes T cells, B cells, and macrophages, the effect of using crocodile blood alone to enhance immunity is also quite limited. Therefore, to avoid the toxic side effects of existing immunosuppressants, it is urgent to develop crocodile blood and to research and develop more effective, safe, and immune-regulating new products to improve the immunity of people with weak constitutions, or to enhance athletic performance and fatigue resistance, thus significantly improving people's quality of life and work. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the problems of toxic side effects of existing immunosuppressants, the limited effect of crocodile blood in improving immunity, and the lack of more immune-modulating products. The present invention provides a crocodile blood composition for improving immunity and its application.

[0006] The first objective of this invention is to provide a composition that enhances immunity.

[0007] A second object of the present invention is to provide a method for preparing the above-described composition.

[0008] A third object of the present invention is to provide applications of the above-described composition.

[0009] The fourth objective of this invention is to provide a product.

[0010] The above-mentioned objective of this invention is achieved through the following technical solution: This invention provides a composition for enhancing immunity, comprising the following ingredients by weight: 30-60 parts of crocodile blood plasma powder, 1-7 parts of licorice, 5-10 parts of asparagus, 3-10 parts of schisandra, 4-10 parts of codonopsis, 8-15 parts of ganoderma, 2-8 parts of American ginseng, and 1-5 parts of vitamin C.

[0011] The present invention analyzes the components of crocodile blood, showing that the most abundant component in crocodile plasma is amino acids (accounting for more than 60%), followed by alkaloids and nitrogenous bases (such as hypoxanthine, nicotinamide, etc.). It contains abundant amino acids and biologically active nitrogenous compounds, which help regulate oxidative stress and immune response. Based on this, a composition was developed that enhances the effects of crocodile blood and improves immunity. This composition contains crocodile blood plasma powder, licorice, asparagus, schisandra, codonopsis, ganoderma, American ginseng, and vitamin C. It is rich in various active substances, such as hypoxanthine, inosine, and adenosine in crocodile blood, which are closely related to purine metabolism and can regulate oxidative stress and immune response; glycyrrhizic acid and glycyrrhizic polysaccharides in licorice, and asparagine acidic polysaccharides in asparagus, can promote the increase of T cell count and immune organ index; schisandra polysaccharides in schisandra have significant immune-enhancing potential; polysaccharides in codonopsis and American ginseng can activate and induce the proliferation of T and B lymphocytes, thereby enhancing immunity; and ganoderma polysaccharides in ganoderma can regulate peritoneal macrophage activity and enhance the body's immunity. Through the synergistic effect of each component, the effects of crocodile blood are enhanced, resulting in a significant immune-enhancing effect. This invention provides more sources and basis for preparing more immune-boosting products containing crocodile blood, can enhance the immune-boosting effect of crocodile blood, and better utilize the effects of the nutrients and rich bioactive substances in crocodile blood.

[0012] Preferably, the composition contains the following raw materials by weight: 40-50 parts of crocodile blood plasma powder, 3-5 parts of licorice, 7-9 parts of asparagus, 5-7 parts of schisandra, 6-8 parts of codonopsis, 10-12 parts of ganoderma, 4-6 parts of American ginseng, and 2-4 parts of vitamin C.

[0013] Preferably, the method for preparing the crocodile plasma powder is as follows: take crocodile blood, centrifuge and collect the supernatant to obtain plasma, perform vacuum freeze-drying, and then pulverize to obtain crocodile plasma powder.

[0014] The present invention provides a method for preparing the above composition: licorice, codonopsis, asparagus, schisandra, American ginseng and ganoderma are dried and pulverized, mixed in proportion, and boiled in water at a material-to-liquid ratio of 1:1-15 g / mL for 1-2 hours. The mixture is filtered to obtain a filtrate, extracted 2-4 times, and the filtrates are combined and concentrated to obtain a mixed concentrate. Then, crocodile blood plasma powder, the mixed concentrate and vitamin C are mixed in proportion to obtain the final product.

[0015] Preferably, the ratio of licorice, codonopsis, asparagus, schisandra, American ginseng, and ganoderma to water is 1:8-15 g / mL.

[0016] The present invention provides the application of the above composition in enhancing the body's immunity.

[0017] This invention provides the use of the above composition in the preparation of products that enhance immunity.

[0018] The present invention provides the use of the above composition in the preparation of immune enhancers.

[0019] The present invention also provides a product comprising the above-described composition.

[0020] Preferably, the product further includes excipients.

[0021] More preferably, it also contains 10-15 parts of excipients.

[0022] More preferably, the excipient is starch, corn flour, or microcrystalline cellulose.

[0023] The present invention has the following beneficial effects: This invention provides a composition that enhances the efficacy of crocodile blood and improves immunity. The composition uses crocodile blood plasma powder as the main ingredient, combined with traditional Chinese medicine ingredients such as licorice, codonopsis, asparagus, schisandra, American ginseng, and ganoderma. These multiple active ingredients synergistically enhance the immune-boosting effects of crocodile blood, promoting the release of immunomodulatory factors TNF-α and IL-2, increasing the total number of spleen and lymphocytes, as well as the volume of splenic white pulp and lymph node cortex, reducing immunosuppression, and significantly improving lymphocyte proliferation. The composition provided by this invention uses green, safe, and nutritious raw materials, significantly enhancing the effects of crocodile blood and strengthening the body's immunity, thus providing more avenues for the development and utilization of rare species resources. Attached Figure Description

[0024] Figure 1 This is a full scan of the positive and negative ions in crocodile blood.

[0025] Figure 2 Effects of crocodile blood composition on TNF-α levels in mouse serum.

[0026] Figure 3Effects of crocodile blood composition on IL-2 levels in mouse serum.

[0027] Figure 4 This is a pathological section of a mouse liver (hematoxylin-eosin).

[0028] Figure 5 Effect of crocodile blood composition on inhibiting the proliferation of spleen lymphocytes in immunized mice. Detailed Implementation

[0029] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0030] Unless otherwise specified, all reagents and materials used in the following examples are commercially available. The crocodile blood, licorice, asparagus root, schisandra fruit, codonopsis root, ganoderma lucidum, American ginseng, vitamin C, and starch used are all commercially available products. The unit of weight in this invention can be kilograms or other similar units.

[0031] Example 1: Preparation and Composition Analysis of Crocodile Plasma Crocodile blood was placed in a high-speed centrifuge, and the supernatant was collected after centrifugation to obtain plasma. The plasma was then freeze-dried under vacuum and pulverized to obtain crocodile plasma powder for later use. Subsequently, the chemical composition of the crocodile plasma was analyzed using ultra-high performance liquid chromatography-orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS).

[0032] The total ion chromatograms obtained under positive and negative ion ionization modes are as follows: Figure 1 As shown in Table 1, the measured molecular weights of the detected compounds were matched with the theoretical values ​​using ThermoXcalibur 4.1 software, and the compounds were then searched in the CompoundDiscoverer mz-Cloud database for identification. The results show that the mass deviation of all compounds was controlled within ±5 PPM.

[0033] The results showed that 21 compounds were identified in crocodile blood, mainly including amino acids, polyols, fatty acids, and aromatic compounds. Table 1 shows that the most abundant components were amino acids (32.36%) and polyols (13.95%). The most abundant compound was 2,2'-methylenebis(4-methyl-6-tert-butylphenol) (28.72%), followed by isoleucine (24.50%), pentane-1,2,3,4,5-pentaol (13.95%), DL-arginine (6.86%), and adenosine (3.67%).

[0034] Table 1. Chemical components of crocodile blood obtained by analysis using ultra-high performance liquid chromatography-orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap-MS).

[0035] Table 1 shows that the most abundant component in crocodile blood is amino acids (accounting for over 60%), followed by alkaloids and nitrogenous bases (such as hypoxanthine and nicotinamide). Compounds such as hypoxanthine, inosine, and adenosine (0.71%) are closely related to purine metabolism and have been reported to regulate oxidative stress and immune responses. This demonstrates that crocodile blood contains abundant amino acids and bioactive nitrogenous compounds, providing a biochemical basis for the effectiveness of subsequent compound formulations.

[0036] Example 2 Preparation of different compositions Different compositions containing crocodile blood were prepared according to the dosage ratios in Table 2 below. The preparation steps of the compositions are as follows: (1) Place the crocodile blood in a high-speed centrifuge, centrifuge and collect the supernatant to obtain plasma, freeze dry under vacuum, and then pulverize to obtain crocodile plasma powder for later use.

[0037] (2) Water extraction treatment: Glycyrrhiza uralensis, Codonopsis pilosula, Asparagus cochinchinensis, Schisandra chinensis, Panax quinquefolius and Ganoderma lucidum were dried and pulverized. They were mixed at a ratio of 1:10 (g / mL) and boiled in water for 1.5 h. The mixture was then filtered to obtain a filtrate. The filtrate was concentrated to obtain a mixed concentrate.

[0038] (3) The crocodile blood plasma powder, mixed concentrate, vitamin C and excipients (starch, corn flour or cellulose) are mixed in proportion by weight and then granulated by a granulator to obtain the composition.

[0039] Table 2. Dosage ratios of different compositions

[0040] Meanwhile, following the above method, different comparative compositions were prepared with reference to the component amounts in Table 3 for the comparative group, and were prepared for later use.

[0041] Table 3. Proportion of raw materials used in each comparison group

[0042] Example 3: Thymus Index and Spleen Index Test (1) The compositions (compositions 1-3 and control groups 1-7) prepared in Example 2 were ground into powder and then prepared into solutions of different concentrations with physiological saline.

[0043] (2) Setting up a model of low immunity: Sixty SPF-grade Kunming mice, weighing 20-22 g, were selected, with half males and half females. Five mice were randomly selected as the control group, and the remaining mice were injected intraperitoneally with cyclophosphamide at a dose of 50 mg / kg to establish an immunodeficiency model. After four consecutive days of injection, the mice were randomly divided into 11 groups, with half females and half males. Healthy mice were fed saline while the remaining mice were induced to take cyclophosphamide orally.

[0044] (3) Grouping of animals: The study was divided into a control group and a control group. Mice were given physiological saline by gavage: 0.2 mL / 10 g.

[0045] Control group: Immunocompromised mice were treated with solutions prepared from compositions 1-3 at a dose of 100 mg / kg. -1 Administer gavage.

[0046] Control group: Mice with weakened immune systems were treated with solutions prepared in control groups 1-7, at a dose of 100 mg / kg. -1 Administer gavage.

[0047] Feeding continued for 40 days.

[0048] (4) Detection of thymus index and spleen index The mice were administered the medication by gavage once daily for 40 consecutive days. After the administration was completed, the mice were weighed, euthanized, and the thymus and spleen were aseptically removed. The thymus and spleen were rinsed with physiological saline to remove blood, blotted dry with filter paper, and weighed. The thymus index and spleen index were calculated using the following formulas: Thymus index (mg / g) = thymus weight / body weight; Spleen index (mg / g) = spleen weight / body weight.

[0049] The results are shown in Table 4 below. The thymus and spleen indices of mice in the compositions 1-3 groups were higher than those in the control group and the model group. Furthermore, the thymus and spleen indices of the control group were also higher than those of the model group. This series of data strongly demonstrates that compositions 1-3 exhibited the best effect in improving immunity, while the control group, although also having an immune-enhancing effect, was significantly less effective than the composition groups. Further observation of the control group revealed that when one or two of the active ingredients such as licorice, asparagus, schisandra, codonopsis, ganoderma, and American ginseng were missing, or when they were replaced with other traditional Chinese medicine components with similar effects, the thymus and spleen indices shown in the experiment were not as good as those in the composition groups. From the data in Table 4, among the composition groups 1-3, composition 2 had the highest thymus index, while composition 3 had the highest spleen index.

[0050] Table 4. Thymus Index and Spleen Index

[0051] Example 4 Cytokine Level Assay After administration to the experimental mice in Example 3, blood was collected from the inner canthal vein and incubated overnight at 4°C. Serum was then separated. The levels of TNF-α and IL-2 in the serum of each group of experimental mice were detected using an ELISA kit. IL-2 is a regulatory cytokine that promotes the proliferation and differentiation of T cells and B cells, and can be used to evaluate the body's immune function. TNF-α is a typical Th1 cytokine that plays a positive regulatory role in various Th1-type inflammatory diseases. Detecting IL-2 and TNF-α can analyze the role of immune regulation in mice.

[0052] The measurement results are as follows Figures 2-3 As shown in Table 5, the IL-2 value of the composition group is significantly higher than that of ( P <0.01) control group and model group. Meanwhile, although the IL-2 value of the control group was significantly ( P The IL-2 level (<0.01) was better than the model group, but slightly inferior to the combination group. IL-2, as a regulatory cytokine, plays an important role in promoting the proliferation and differentiation of T cells and B cells; a higher IL-2 level indicates a stronger immune-boosting effect. From this perspective, the control group also showed an immune-boosting effect, but the degree of effect was not as strong as the combination group. Among the combination groups, Composition 3 had the highest IL-2 value.

[0053] Table 5. Results of TNF-α and IL-2 detection

[0054] TNF-α can selectively kill tumor cells and has immunomodulatory effects, inducing the secretion of other immune factors. As shown in Table 5, the TNF-α levels in mice in the cyclophosphamide-induced immunosuppression model were significantly lower than (…). P <0.01) blank group, indicating that the TNF-α cytokine level in immunosuppressed mice was decreased; compared with the model group, the TNF-α cytokine level concentration in both the composition group and the control group was significantly increased ( P <0.01). Based on this principle, the data comparison above clearly leads to the conclusion that the combined composition group exhibits the best effect in immunomodulation, while the control group, although not as effective as the combined composition group, still demonstrates a certain degree of immunomodulatory effect. Among compositions 1-3, composition 3 shows the most prominent immunomodulatory effect, exhibiting the best immunomodulatory effect. This fully demonstrates that in the crocodile blood-containing composition, each component is indispensable, and these components also exhibit unexpected synergistic effects, working together to better enhance the efficacy of immunity.

[0055] The above experiments found that crocodile blood composition 1-3 can increase the levels of TNF-α and IL-2 in the serum of immunosuppressed mice. This result indicates that crocodile blood composition can enhance cellular immunity and immune regulation in immunosuppressed mice by increasing the levels of these cytokines.

[0056] Example 5: Application of crocodile blood composition in promoting the recovery of immune organs and enhancing immune function 1. Effects on immune cell proliferation Twenty-four SPF-grade Kunming mice, weighing 20-22 g, were randomly divided into four groups: a blank control group (equal volume of physiological saline); a cyclophosphamide group (50 mg / kg); and a control group (50 mg / kg of cyclophosphamide). -1 Example group (crocodile blood composition group 1) (100 mg·kg) -1 ); Cyclophosphamide (50 mg·kg) -1 ) + Crocodile Blood Combination 1 (100 mg·kg) -1 )Group.

[0057] Ten mice were injected intraperitoneally with cyclophosphamide at a dose of 50 mg / kg for four consecutive days. They were then divided into a cyclophosphamide group and a cyclophosphamide + crocodile blood combination group. The mice in the cyclophosphamide group were sacrificed by cervical dislocation and weighed 24 hours after the last administration.

[0058] Except for the cyclophosphamide group, the other groups were administered the specified dose via intraperitoneal injection once daily for 5 consecutive days. Twenty-four hours after the last administration, mice were sacrificed by cervical dislocation and weighed. Three mice from each group were harvested, and tissue samples from the spleen and lymph nodes were accurately weighed. Cell suspensions were prepared according to standard procedures, and the cells were counted under a microscope to calculate the total number of cells in each organ.

[0059] The results are shown in Table 6. The cyclophosphamide group caused a significant loss of total spleen and lymphocyte count. The immunosuppressive effect of cyclophosphamide was significantly reduced. The use of crocodile blood composition group 1 resulted in a higher cell count in both organs than the normal level in the control group, indicating an enhanced effect of promoting immune cell proliferation. Regarding the immunosuppressive effect caused by cyclophosphamide, crocodile blood composition group 1 also restored the total number of spleen and lymph node cells to a level slightly exceeding the normal level in the control group, indicating that crocodile blood composition can replenish the reserve of immune cells.

[0060] Table 6. Observation on the effect of crocodile blood composition on cell number in immune organs

[0061] 2. Effects on the morphology and structure of mouse immune organs In the blank group, cyclophosphamide group, cyclophosphamide + crocodile blood composition group 1 and crocodile blood composition group 1, livers of 3 mice were randomly selected from each group, rinsed with pre-cooled physiological saline, dried with filter paper, fixed in 4% paraformaldehyde solution, washed and dehydrated, embedded in paraffin, sectioned, stained with hematoxylin and eosin (HE), mounted, and images were collected and tissue morphology was observed under an optical microscope.

[0062] like Figure 4 As shown, after HE staining, liver sections were examined under a light microscope. The results indicated that the hepatocytes in the control group had normal morphology and uniform sinusoidal spaces; focal infiltration of inflammatory cells was observed in the liver tissue of the cyclophosphamide group. Compared with the cyclophosphamide group, the cyclophosphamide + crocodile blood combination group 1 could repair and improve cyclophosphamide-induced liver damage in mice, reduce focal infiltration of inflammatory cells, and the number of focal infiltrations of inflammatory cells in the crocodile blood combination group 1 was less than that in the control group.

[0063] 3. Effects on total white pulp volume and total cortical volume of lymph nodes in mice Next, under a light microscope, the correlation square test grids were randomly superimposed on the cross-sections of the spleen and lymph nodes, and the number of test points located in the spleen and lymph nodes was counted sequentially and completely (P0). Then, the number of test points located in the white pulp of the spleen (including splenic nodules and periarterial lymphatic sheaths) and the cortex of the lymph nodes (including lymphatic nodules and accessory cortical areas) was counted (P0). S According to P) S The ratio of V0 to P0 represents the volume ratio of the feature (white pulp / cortex) within the reference space (spleen / lymph node). Multiplying this ratio by the total volume of the reference space (V0 = W / D, D = 1.04, usually taken as 1) yields the total volume of the feature within the reference space (V0). S ).

[0064] The results are shown in Table 7. Cyclophosphamide reduced the volume of spleen white pulp and lymph node cortex in mice, indicating its immunosuppressive effect. The total volume of spleen white pulp and lymph node cortex in group 1, which used only the crocodile blood composition, was higher than the normal level in the control group. The crocodile blood composition reversed the atrophy of cyclophosphamide and even restored the volume of spleen white pulp and lymph node cortex to slightly exceed that of the normal control group, suggesting that it has the potential to promote the recovery of immune organs and enhance immune function.

[0065] Table 7. Effects of crocodile blood composition on total white pulp volume and total lymph node cortex volume in mice.

[0066] Example 6: Effect of crocodile blood composition on the proliferation of splenic lymphocytes in immunosuppressed mice In Example 5 above, three mice were used in each group. Twenty-four hours after the drug administration, the mice were euthanized by cervical dislocation. The euthanized mice were then disinfected by soaking in 70% ethanol solution for 10 minutes. After soaking, the spleens of the disinfected mice were harvested in a sterile operating table to prepare lymphocytes. The viable cells of the prepared lymphocytes were counted using the trypan blue method, and the cell concentration was adjusted to 1 × 10⁻⁶ cells / mL using RPMI-1640 medium containing 10% fetal bovine serum. 6 Cells were seeded at 100 μL / well in 96-well plates (3 replicates per group), and simultaneously stimulated with 7.5 μg / mL ConA (test wells), with unstimulated wells (blank wells) as non-stimulated wells. After incubation at 37 ℃ and 5% CO2 for 68 h, 5 mg / mL LMT (10 μL / well) was added, and the cells were incubated for another 4 h. After incubation, 150 μL of dimethyl sulfoxide solution was added, the cells were shaken several times, and then incubated in the dark at room temperature for 15 min. The absorbance of each well was measured at 570 nm using a microplate reader.

[0067] Absorbance calculation formula: P = A ConA - A Control ; In the formula: P represents the lymphocyte proliferation capacity; A ConA To measure the absorbance of the well sample after adding concanavalin A; A Control The absorbance is for the well sample without the addition of concanavalin A stimulation.

[0068] The results are as follows Figure 5 As shown in Table 8, the T lymphocyte proliferation capacity of mice in the cyclophosphamide immunosuppression model group was significantly lower than that in the blank group ( P <0.01 indicates that the proliferation capacity of T lymphocytes in immunosuppressed mice is reduced. Compared with the model group, the lymphocyte proliferation capacity of both the crocodile blood composition 1 group and the cyclophosphamide + crocodile blood composition 1 group was significantly increased ( P The result was <0.01, indicating that the crocodile blood composition could improve the proliferation of mouse spleen lymphocytes induced by mitogen ConA.

[0069] Table 8. Effects of crocodile blood composition on inhibiting the proliferation of splenic lymphocytes in immunized mice.

[0070] T and B lymphocytes are mainly distributed in splenic lymphocytes and are an important component of the immune system. The proliferation and differentiation of splenic lymphocytes are also a crucial stage in the immune response process; therefore, the level of splenic lymphocyte proliferation and differentiation can be used to assess the body's immune function. The mitogen concanavalin A (ConA) stimulates T cell proliferation and differentiation. This experiment utilizes ConA-induced lymphocyte proliferation to evaluate T lymphocyte proliferation and differentiation, and thus to evaluate the effect of the crocodile blood composition on cellular immune function. T lymphocytes are the main effector cells mediating the body's cellular immune response. The results of this experiment show that the crocodile blood composition can promote ConA-stimulated T lymphocyte proliferation, and by enhancing the proliferative capacity of T lymphocytes, it can improve the cellular immune function of immunosuppressed mice.

[0071] The above studies show that the analysis of the components of crocodile blood reveals that it contains abundant amino acids and bioactive nitrogenous compounds, providing a biochemical basis for its potential applications in metabolic regulation or cardiovascular function regulation. Based on this, the present invention has developed an immune-enhancing composition that enhances the effects of crocodile blood. This composition contains crocodile blood plasma powder, licorice, asparagus, schisandra, codonopsis, ganoderma, American ginseng, and vitamin C. Crocodile blood is scientifically compounded with specific traditional Chinese medicine ingredients. The composition is rich in various active substances that enhance immunity. For example, compounds such as hypoxanthine, inosine, and adenosine in crocodile blood are closely related to purine metabolism and can regulate oxidative stress and immune response. Betaine and L-phenylalanine are generally related to cardiovascular health and systemic regulation. Glycyrrhizic acid and licorice polysaccharides in licorice and asparagine acidic polysaccharides in asparagus can promote the increase of T cell number and immune organ index. Schisandra polysaccharides in schisandra have significant immune-enhancing potential. Polysaccharides in codonopsis and American ginseng can activate and induce the proliferation of T and B lymphocytes, thereby enhancing immunity. Ganoderma polysaccharides in ganoderma can regulate the activity of peritoneal macrophages and enhance the body's immunity. This composition, through rational compounding, synergistically combines traditional Chinese medicine with crocodile blood, further enhancing the immune-boosting effect of crocodile blood, and providing more sources and basis for the preparation of more immune-boosting products containing crocodile blood.

[0072] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A crocodile blood composition, characterized in that, By weight, it contains: 30-60 parts crocodile blood plasma powder, 1-7 parts licorice, 5-10 parts asparagus, 3-10 parts schisandra, 4-10 parts codonopsis, 8-15 parts ganoderma, 2-8 parts American ginseng, and 1-5 parts vitamin C.

2. The composition according to claim 1, characterized in that, By weight, it contains: 40-50 parts crocodile blood plasma powder, 3-5 parts licorice, 7-9 parts asparagus, 5-7 parts schisandra, 6-8 parts codonopsis, 10-12 parts ganoderma, 4-6 parts American ginseng, and 2-4 parts vitamin C.

3. The composition according to claim 1, characterized in that, The preparation method of the crocodile plasma powder is as follows: take crocodile blood, centrifuge and collect the supernatant to obtain plasma, perform vacuum freeze drying, and then pulverize to obtain crocodile plasma powder.

4. A method for preparing the composition according to any one of claims 1 to 3, characterized in that, Licorice, Codonopsis, Asparagus, Schisandra, American ginseng and Ganoderma lucidum are dried and pulverized separately, mixed in proportion, and boiled in water at a material-to-liquid ratio of 1:(1-15) g / L for 1-2 hours. The filtrate is filtered and extracted 2-4 times. The filtrates are combined and concentrated to obtain a mixed concentrate. Then, crocodile blood plasma powder, mixed concentrate and vitamin C are mixed in proportion to obtain the final product.

5. The preparation method according to claim 4, characterized in that, The ratio of licorice, codonopsis, asparagus, schisandra, American ginseng, and ganoderma to water is 1:8-15 g / mL.

6. Use of the composition according to any one of claims 1 to 3 in the preparation of products that enhance immunity.

7. Use of the composition according to any one of claims 1 to 3 in the preparation of an immunomodulator.

8. A product characterized in that, The composition comprising any one of claims 1 to 3.

9. The product according to claim 8, characterized in that, The product also contains auxiliary materials.

10. The product according to claim 9, characterized in that, The excipients are starch, corn flour, or microcrystalline cellulose.

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