A fluorescence imaging device capable of automatic distance adjustment

Abstract

The present application relates to a kind of fluorescence imaging device capable of automatically adjusting distance, including imaging device body, pedestal and the carrier of being arranged in the top of pedestal. Carrier is used to place sample test tube to be detected, and the imaging lens of imaging device body is downward to carrier to obtain fluorescence signal, and is handled by microprocessor. Carrier is connected with adjusting assembly, including with the fixed tooth plate of carrier, meshing gear, servo motor and connecting rod. Microprocessor is communicated with servo motor, and servo motor is rotated based on real-time fluorescence signal signal-to-noise ratio control, and driving gear tooth plate adjusts carrier height, when signal-to-noise ratio reaches preset threshold, stop adjusting. Compared with prior art, the present application realizes the automatic optimization of imaging distance, compact structure, can be based on fluorescence signal quality real-time feedback and automatically adjust imaging focal length, effectively improve the accuracy and efficiency of fluorescence imaging.

Description

Technical Field

[0001] This invention relates to the field of fluorescence imaging device technology, and in particular to a fluorescence imaging device capable of automatic distance adjustment. Background Technology

[0002] Fluorescence imaging technology, as an important tool in biomedical detection, is widely used in cell analysis, drug screening, and other fields due to its high sensitivity and specificity. However, existing fluorescence imaging devices still have significant limitations in achieving high signal-to-noise ratio imaging.

[0003] Specifically, prior art document CN115607103A discloses a near-infrared wavelength-tunable fluorescence imaging device, which adjusts the output wavelength of the light source through a wavelength tuning module such as a grating or prism to optimize the signal-to-noise ratio of the fluorescence signal. However, this scheme relies on a complex spectral tuning mechanism, resulting in a cumbersome system structure, high cost, and a long wavelength scanning and comparison process, usually more than 2 minutes, which is difficult to meet the needs of real-time rapid detection.

[0004] On the other hand, prior art document CN113670880A discloses a fluorescence microscopy imaging device that integrates a metalens with an image sensor to expand the field of view. However, the height adjustment of the slide relies entirely on manual operation of the adjustment knob to drive the displacement lever, which has problems such as large subjective error, poor repeatability, and low efficiency, and cannot achieve automated and precise control of the imaging distance.

[0005] In summary, the core problem with existing technologies lies in the lack of a control mechanism that can automatically, quickly, and accurately optimize the imaging distance, thereby ensuring that the signal-to-noise ratio of the fluorescence image is always at its optimal state in real time. The CN115607103A device improves the signal-to-noise ratio by indirectly tuning the wavelength, but at the expense of response speed and system simplicity; the CN113670880A device completely lacks automatic distance adjustment functionality.

[0006] Therefore, there is an urgent need for a compact and efficient imaging device that can automatically adjust the imaging focal length based on real-time feedback of fluorescence signal quality. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of the prior art and provide a compact, efficient imaging device that can automatically adjust the imaging focal length based on real-time feedback of fluorescence signal quality.

[0008] The objective of this invention can be achieved through the following technical solutions: This invention provides an automatically adjustable fluorescence imaging device, comprising an imaging device body, a base, and a carrier disposed on top of the base. The bottom of the imaging device body is fixedly connected to the base. The carrier is used to place a sample tube to be detected for fluorescence. The imaging lens of the imaging device body faces downward toward the carrier to acquire the fluorescence signal corresponding to the sample tube, which is then processed by a microprocessor in the imaging device body. An adjustment component for driving its movement is connected to the carrier. The adjustment component includes a toothed plate fixedly connected to the carrier, a gear meshing with the toothed plate, and a servo motor driving the gear to rotate. The output end of the servo motor is fixedly connected to the gear via a connecting rod. The microprocessor is communicatively connected to the servo motor. Based on the signal-to-noise ratio of the fluorescent signal acquired in real time, the microprocessor instructs the servo motor to rotate, thereby achieving height adjustment of the vehicle. When the signal-to-noise ratio reaches a preset threshold, the microprocessor instructs the servo motor to stop rotating.

[0009] Furthermore, a distance sensor is also provided on one side of the imaging lens. The distance sensor is used to obtain distance information between the imaging lens and the sample tube on the carrier. The distance sensor is communicatively connected to the microprocessor.

[0010] Furthermore, the microprocessor, based on preset target height information of the vehicle or real-time distance between the vehicle and the imaging lens fed back by a distance sensor, instructs the servo motor to rotate, thereby adjusting the vehicle's height. When the vehicle reaches the target height or the real-time distance reaches a preset focal length range, the microprocessor, using the real-time signal-to-noise ratio of the fluorescence signal, instructs the servo motor to rotate, thus adjusting the vehicle's height. When the signal-to-noise ratio reaches a preset threshold, the microprocessor instructs the servo motor to stop rotating. In other words, the overall process involves first adjusting the height to a preset range, and then fine-tuning based on the signal-to-noise ratio.

[0011] Furthermore, the carrier includes at least one trough for placing sample tubes.

[0012] Furthermore, the vehicle has a guide hole in the middle, and the imaging device body has a guide post that matches the guide hole. The guide post is arranged in the vertical direction and is used to guide the vehicle when it is vertically displaced.

[0013] Furthermore, the microprocessor is a single-chip microcomputer or a processor based on x86, ARM, or RISC-V architecture.

[0014] Furthermore, the sample tubes to be detected by fluorescence placed in the carrier are transparent glass or plastic tubes, and the samples in the sample tubes to be detected by fluorescence are samples pre-added with fluorescent labeling substances.

[0015] Furthermore, the inner wall of the tank is a matte finish, and the tank is provided with at least one set of limiting columns that can match sample tubes of various sizes. Each set of limiting columns includes four flexible silicone limiting columns. When the sample tube is placed horizontally in the tank, the four flexible silicone limiting columns limit the position of the sample tube.

[0016] Furthermore, the side of the toothed plate without teeth is fixedly connected to the side of the vehicle.

[0017] Furthermore, the main body of the servo motor is connected to the main body of the imaging device, and the output shaft of the servo motor is connected to the middle part of the gear.

[0018] In an optional embodiment of the present invention, the surface of the connecting rod is further provided with a cleaning assembly. The cleaning assembly includes a drive pulley fixedly connected to the surface of the connecting rod, a rotating pulley connected to the drive pulley via a belt, a connecting block fixedly connected inside the rotating pulley, a sleeve fixedly connected inside the connecting block, and a cleaning block fixedly connected inside the connecting block. When the connecting rod rotates, the drive pulley drives the rotating pulley to rotate, thereby causing the cleaning block to clean the dustproof screen on the surface of the ventilation opening of the imaging device body through the connecting block. The ventilation opening is designed to be inclined, so that the dust removed during cleaning slides down the inclined surface, preventing it from entering the interior of the imaging device body, thereby keeping the interior clean and extending the service life of the equipment.

[0019] According to an optional embodiment of the present invention, a support assembly is provided on one side of the top of the base. The support assembly includes a crossbar, a hollow block movably sleeved on the surface of the crossbar, a telescopic block slidably connected inside the hollow block, a threaded hole opened inside the telescopic block and extending to the outside of the hollow block, and a positioning bolt threadedly connected inside the threaded hole. Return torsion springs are fixedly connected to both sides of the surface of the crossbar, and the other end of the return torsion spring is fixedly connected to the inner wall of a receiving groove opened on the base. Through the torque of the return torsion spring, the hollow block can rotate on the surface of the crossbar, causing the telescopic block to adjust its height and be fixed by the positioning bolt, thereby supporting the bottom of the vehicle and ensuring the stability of the vehicle during movement.

[0020] Based on an optional embodiment of the present invention, a fixing block is fixedly connected inside the vent, a bearing is fixedly connected to the front of the fixing block, and a positioning rod is fixedly connected inside the bearing; the sleeve of the cleaning component is fitted onto the surface of the positioning rod, and the rotating pulley is limited by the cooperation of the positioning rod and the bearing to prevent it from shaking during rotation; the inclined design of the vent works in conjunction with the rotational movement of the cleaning block to effectively guide dust outward and avoid secondary pollution.

[0021] According to an optional embodiment of the present invention, a limiting block is slidably connected to the surface of the toothed plate, and the back side of the limiting block is fixedly connected to the front side of the imaging device body; a through hole is opened at the top of the limiting block, the front side of the connecting rod penetrates the front side of the limiting block, and a round block is fixedly connected to the front side of the connecting rod; through the sliding connection between the limiting block and the toothed plate, and the limiting of the connecting rod by the round block, the left and right swaying or up and down displacement of the toothed plate during the movement is constrained, thereby improving the accuracy and stability of the vehicle movement.

[0022] Based on the optional embodiments of the present invention, mounting ears are fixedly connected to both the front and back sides of the toothed plate. The mounting ears are reinforced to the side of the carrier by bolts or welding. The mounting ears are made of high-strength metal material and are designed in an L-shape to increase the contact area, thereby dispersing the stress generated when the carrier moves, preventing the toothed plate from loosening at the connection with the carrier, and ensuring transmission efficiency and service life.

[0023] Based on the optional embodiments of the present invention, the inner wall of the tank is a matte wall, and its surface is sandblasted to reduce reflection; the tank is provided with at least one set of limiting posts, each set of limiting posts including 4 flexible silicone limiting posts, the flexible silicone limiting posts are symmetrically distributed, when the sample tube is placed horizontally in the tank, the elastic deformation of the flexible silicone adapts to the test tubes of different sizes, so as to achieve stable positioning and avoid scratching the surface of the test tube.

[0024] According to an optional embodiment of the present invention, motor slots are provided on the front and back of the imaging device body, and the main body of the servo motor is embedded and fixed inside the motor slots; the size of the motor slots matches the servo motor, the inner wall of the slots is provided with heat dissipation fins, and the bottom of the slots is provided with cable holes for wiring; through the encapsulation design of the motor slots, not only is space saved, but the heat dissipation efficiency and operational stability of the servo motor are also improved.

[0025] According to an optional embodiment of the present invention, the bottom of the base is fixedly connected with anti-slip legs, and the number of anti-slip legs is four, which are located at the four corners of the base respectively; each anti-slip leg is made of rubber or silicone material, and its bottom is provided with concave and convex patterns to increase friction, and its height is adjustable to adapt to different work surfaces through a threaded structure, preventing the device from sliding or shifting during operation, and ensuring the safety and accuracy of the imaging process.

[0026] Based on the optional embodiments of the present invention, the reset torsion spring is preset with an initial torque, is made of spring steel, and has a rust-proof surface. When the vehicle height is adjusted, the reset torsion spring drives the hollow block to automatically reset to the initial position through elastic restoring force. The depth of the receiving groove matches the length of the crossbar to ensure smooth movement of the torsion spring. This design simplifies the operation process of the support assembly, improves adjustment efficiency, and reduces manual intervention.

[0027] Compared with the prior art, the present invention has the following beneficial effects: The automatic distance-adjusting fluorescence imaging device provided by this invention integrates a precision adjustment component driven by a servo motor and a gear plate, and employs a closed-loop feedback control strategy based on the real-time fluorescence signal-to-noise ratio to achieve automated and intelligent adjustment of the carrier height. This device continuously optimizes the distance between the imaging lens and the sample during the detection process, ensuring that the fluorescence signal is always in the optimal acquisition state, thereby significantly improving the accuracy and reliability of the detection. Simultaneously, it avoids the problems of low efficiency, poor repeatability, and human error associated with traditional manual distance adjustment, greatly enhancing detection efficiency and automation. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the main structure of the present invention.

[0029] Figure 2 This is a schematic diagram of the detached structure of the cleaning component and the main body of the present invention.

[0030] Figure 3 This is a schematic diagram of the position of the servo motor output shaft of the present invention.

[0031] Figure 4 This is a schematic diagram of the hollow block of the present invention.

[0032] Figure 5 This is a schematic diagram of the telescopic block of the present invention.

[0033] Figure 6 This is a schematic diagram of the limiting block of the present invention.

[0034] Figure 7 This is a schematic diagram of the toothed plate of the present invention.

[0035] Figure 8 For the present invention Figure 2 Enlarged view of point A in the middle.

[0036] In the attached diagram: 0. Imaging lens; 1. Imaging device body; 2. Base; 3. Carrier; 4. Ventilation port; 5. Dustproof net; 6. Adjustment component; 61. Gear; 7. Tooth plate; 8. Connecting rod; 9. Servo motor; 10. Cleaning component; 11. Drive pulley; 12. Rotating pulley; 13. Connecting block; 14. Sleeve; 15. Cleaning block; 16. Support component; 17. Crossbar; 18. Hollow block; 19. Telescopic block; 20. Threaded hole; 21. Positioning bolt; 22. Receiving groove; 23. Return torsion spring; 24. Fixing block; 25. Bearing; 26. Positioning rod; 27. Motor groove; 28. Anti-slip leg; 29. ​​Limiting block; 30. Through hole; 31. Round block; 32. Rubber pad; 33. Mounting ear; 34. Guide post; 35. Groove. Detailed Implementation

[0037] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. Component models, material names, connection structures, circuit structures, control methods, algorithms, and other features not explicitly described in this technical solution are considered common technical features disclosed in the prior art.

[0038] Example 1 This embodiment describes a fluorescence imaging device capable of automatic distance adjustment, see [link to previous document]. Figure 1 The device includes an imaging device body 1, a base 2, and a carrier 3 disposed on top of the base 2. The bottom of the imaging device body 1 is fixedly connected to the base 2. The carrier 3 is used to place the sample tube to be detected for fluorescence. The imaging lens 0 of the imaging device body 1 faces downward toward the carrier 3 to acquire the fluorescence signal corresponding to the sample tube and process it through the microprocessor in the imaging device body 1. An adjustment component 6 for driving its movement is connected to the carrier 3. The adjustment component 6 includes a toothed plate 7 fixedly connected to the carrier 3, a gear 61 meshing with the toothed plate 7, and a servo motor 9 driving the gear 61 to rotate. The output end of the servo motor 9 is fixedly connected to the gear 61 through a connecting rod 8.

[0039] The microprocessor is communicatively connected to the servo motor 9. Based on the signal-to-noise ratio of the fluorescent signal acquired in real time, the microprocessor instructs the servo motor 9 to rotate, thereby achieving height adjustment of the vehicle 3. When the signal-to-noise ratio reaches a preset threshold, the microprocessor instructs the servo motor 9 to stop rotating.

[0040] In a specific implementation, a distance sensor is also provided on one side of the imaging lens 0. The distance sensor is used to obtain the distance information between the imaging lens 0 and the sample tube on the carrier 3. The distance sensor is communicatively connected to the microprocessor.

[0041] In specific implementation, the microprocessor instructs the servo motor 9 to rotate based on the preset target height information of the vehicle or the real-time distance between the vehicle 3 and the imaging lens fed back by the distance sensor, thereby adjusting the height of the vehicle 3. When the vehicle 3 reaches the target height or the real-time distance reaches the preset focal length value range, the microprocessor instructs the servo motor 9 to rotate based on the real-time signal-to-noise ratio of the fluorescence signal, thereby adjusting the height of the vehicle 3. When the signal-to-noise ratio reaches a preset threshold, the microprocessor instructs the servo motor 9 to stop rotating.

[0042] In specific implementation, the carrier 3 includes at least one groove 35 for placing sample tubes. The carrier 3 has a guide hole in its center, and the imaging device body 1 has a guide post 34 that matches the guide hole. The guide post 34 is arranged vertically to guide the carrier 3 during vertical displacement.

[0043] In specific implementation, the microprocessor is a single-chip microcomputer or a processor based on x86, ARM, or RISC-V architecture.

[0044] In practice, the sample tubes to be detected in the carrier 3 are transparent glass or plastic tubes, and the samples in the sample tubes are pre-added with fluorescent markers. The inner wall of the tank 35 is matte, and the tank 35 is equipped with at least one set of limiting posts that match sample tubes of various sizes. Each set of limiting posts includes four flexible silicone limiting posts. When the sample tube is placed horizontally in the tank 35, the four flexible silicone limiting posts limit the position of the sample tube.

[0045] In practice, the side of the toothed plate 7 without teeth is fixedly connected to the side of the carrier 3. The main body of the servo motor 9 is connected to the imaging device body 1, and the output shaft of the servo motor 9 is connected to the middle of the gear 61.

[0046] In this embodiment, the imaging principle of the imaging device body 1 is based on fluorescence imaging technology. Its core process involves excitation light emission, fluorescence signal capture, and intelligent analysis and control. A specific wavelength excitation light source is set at the imaging lens 0, and the excitation light emitted by this source shines downwards onto the sample tube placed on the carrier 3. The fluorescently labeled substance (such as indocyanine green) pre-injected in the tube undergoes an energy transition and emits a longer wavelength fluorescence signal after being irradiated by the excitation light. Subsequently, the imaging lens 0, facing the carrier 3, accurately captures these weak fluorescence signals and converts them into electrical signals. The microprocessor integrated inside the body then processes this electrical signal in real time, evaluating the imaging quality by calculating its signal-to-noise ratio. Based on the signal-to-noise ratio, the microprocessor controls the servo motor 9 of the adjustment structure 6 to drive the gear 61 and the toothed plate 7, thereby precisely adjusting the height of the carrier 3, ultimately ensuring that the imaging lens 0 is always at the optimal focal plane, ensuring a clear, high signal-to-noise ratio fluorescence image.

[0047] In this embodiment, the automatic distance-adjusting fluorescence imaging device uses a microprocessor to control the carrier height to optimize the fluorescence signal acquisition quality in real time. The device mainly consists of an imaging device body 1, a base 2, and a carrier 3. The imaging device body 1 is fixed above the base 2, and the carrier 3 is located on top of the base 2 for placing sample tubes. An imaging lens 0 is mounted on the imaging device body 1, facing downwards towards the carrier 3 to capture the fluorescence signal emitted by the tubes. A microprocessor embedded inside the imaging device body 1 processes and analyzes the fluorescence signal in real time. The adjustment component 6 connects to the carrier 3 and includes a toothed plate 7 fixed to the carrier 3, a gear 61 meshing with the toothed plate 7, and a servo motor 9 driving the gear 61. The output of the servo motor 9 is connected to the gear 61 via a connecting rod 8. When the microprocessor issues a command based on the fluorescence signal-to-noise ratio (SNR), the servo motor 9 rotates, driving the gear 61 to drive the toothed plate 7, causing the carrier 3 to move vertically. The microprocessor continuously monitors the SNR; when the SNR is lower than a preset threshold, the servo motor 9 is activated to adjust the height until the SNR reaches the optimal value, then stops, forming a closed-loop control to ensure imaging stability. This mechanism achieves precise distance adjustment through the coordinated use of mechanical transmission and electronic feedback. See Figure 1 This diagram illustrates the overall layout of the device and the connections between its components.

[0048] In specific implementation, a distance sensor is further integrated and located on one side of the imaging lens 0 to obtain real-time distance information between the lens and the test tubes on the carrier 3. The distance sensor communicates with the microprocessor, which can perform preliminary coarse adjustments to the servo motor 9 based on preset target height or distance feedback commands. When the carrier 3 reaches the target height or distance and enters the preset focal length range, the microprocessor switches to signal-to-noise ratio optimization mode for fine-tuning. A guide hole is provided in the middle of the carrier 3, which cooperates with the guide post 34 on the imaging device body 1. The guide post 34 is set vertically to ensure that the carrier 3 maintains a vertical trajectory when moving, avoiding deviation and improving adjustment accuracy. The carrier 3 has at least one groove 35 for placing sample test tubes. The inner wall of the groove 35 is matte to reduce reflection interference. A set of limiting posts is configured inside the groove 35. Each set of limiting posts includes 4 flexible silicone limiting posts to adapt to test tubes of different sizes. Stable fixation is achieved through flexible limiting. The side of the toothed plate 7 without teeth is fixedly connected to the side of the carrier 3. The main body of the servo motor 9 is connected to the imaging device body 1. The output shaft is connected to the middle of the gear 61 to ensure smooth and reliable transmission. The entire system achieves efficient and high-precision automatic distance adjustment through multi-sensor data fusion and mechanical guidance design, making it suitable for multi-sample fluorescence detection scenarios.

[0049] Example 2 Unlike Embodiment 1, the bottom of the imaging device body 1 is fixedly connected to a base 2, and a carrier 3 is provided on the top of the base 2. Ventilation openings 4 are provided on both the front and back of the imaging device body 1. Adjustment components 6 are provided on both sides of the back of the carrier 3. The adjustment components 6 include a toothed plate 7. The front of the toothed plate 7 is fixedly connected to the back of the carrier 3. A gear 61 is meshed on the back of the toothed plate 7. A connecting rod 8 is fixedly connected inside the gear 61. A servo motor 9 is fixedly connected to the inner side of the connecting rod 8. A cleaning component 10 is provided on the surface of the connecting rod 8.

[0050] Reference Figure 3 As shown in this embodiment: the cleaning component 10 includes a drive pulley 11, the interior of the drive pulley 11 is fixedly connected to the surface of the connecting rod 8, a rotating pulley 12 is provided on the top of the drive pulley 11, the rotating pulley 12 and the drive pulley 11 are connected by belt drive, a connecting block 13 is fixedly connected inside the rotating pulley 12, a sleeve 14 is fixedly connected to the inner side of the connecting block 13, a cleaning block 15 is fixedly connected to the inner side of the connecting block 13, and a support component 16 is provided on the left side of the top center of the base 2.

[0051] Specifically: By setting the cleaning component 10, the connecting rod 8 can rotate to drive the drive pulley 11 to rotate, the drive pulley 11 rotates to drive the rotating pulley 12 to rotate, the rotating pulley 12 rotates to drive the connecting block 13 to rotate, and the connecting block 13 rotates to drive the cleaning block 15 to clean the dustproof net 5 on the surface of the vent 4. Because the vent 4 is inclined, it prevents the dust from entering the imaging device body 1.

[0052] Reference Figure 4 As shown, in this embodiment: the support assembly 16 includes a crossbar 17, which is located on the left side of the center of the base 2. A hollow block 18 is movably sleeved on the surface of the crossbar 17. A telescopic block 19 is slidably connected inside the hollow block 18. A threaded hole 20 is opened inside the telescopic block 19. The threaded hole 20 extends to the outside of the hollow block 18. A positioning bolt 21 is threadedly connected inside the threaded hole 20.

[0053] Specifically: By setting up the support components, the hollow block 18 can be rotated on the surface of the crossbar 17 by the torque of the torsion spring. The rotation of the hollow block 18 causes the telescopic block 19 to rotate. The positioning bolt 21 is removed and the user repositions the telescopic block 19 according to the height of the vehicle 3. The bottom of the vehicle 3 is supported by the rubber pad 32 on the top of the telescopic block 19.

[0054] Reference Figure 2 As shown in this embodiment: a receiving groove 22 is provided on the left side of the center of the base 2, and a reset torsion spring 23 is fixedly connected to both sides of the surface of the crossbar 17. The other end of the reset torsion spring 23 is fixedly connected to the inner wall of the receiving groove 22.

[0055] Specifically, the arrangement of the receiving slot 22 provides a regular space for the crossbar 17 and the return torsion spring 23, reducing unnecessary space occupation and facilitating quick positioning and operation of related components during assembly, maintenance and repair, greatly improving work efficiency. The return torsion spring 23, with its unique torque characteristics, can accurately and stably drive the telescopic block 19, providing reliable support for the bottom of the vehicle 3, effectively ensuring the stability of the vehicle 3 during operation and reducing errors caused by shaking.

[0056] Reference Figure 8 As shown in this embodiment: a fixing block 24 is fixedly connected inside the ventilation opening 4, and a bearing 25 is fixedly connected to the front of the fixing block 24.

[0057] Specifically, the fixing block 24, with its stable structural characteristics, provides solid and reliable support for the bearing 25, ensuring that the bearing 25 maintains a stable position during equipment operation and will not shift due to external forces or equipment vibration. It not only effectively limits the positioning rod 26 and precisely controls its range of motion, but also gives the positioning rod 26 smooth rotational movement capability, allowing the positioning rod 26 to rotate flexibly within a certain angle range, meeting the positioning and movement needs of the equipment under different working conditions, and improving the operational flexibility and adaptability of the equipment.

[0058] Reference Figure 3 As shown in this embodiment: a positioning rod 26 is fixedly connected inside the bearing 25, and the sleeve 14 is located on the surface of the positioning rod 26.

[0059] Specifically: By setting the positioning rod 26, the rotating pulley 12 can be limited by the positioning rod 26 and the bearing 25, so as to prevent the rotating pulley 12 from shaking when rotating.

[0060] Reference Figure 2 As shown in this embodiment: motor slots 27 are provided on the front and back of the imaging device body 1, and anti-slip legs 28 are fixedly connected to the bottom of the base 2.

[0061] Specifically: the motor slot 27 facilitates the installation and fixation of the servo motor 9, and increases the stability of the servo motor 9. The anti-slip leg 28 prevents the imaging device body 1 from sliding or shifting.

[0062] Reference Figure 6 As shown in this embodiment: the surface of the toothed plate 7 is slidably connected to the limiting block 29, and the back side of the limiting block 29 is fixedly connected to the front side of the imaging device body 1.

[0063] Specifically, by setting the limiting block 29, when the toothed plate 7 moves linearly under power to move the carrier 3, the limiting block 29, through its tight sliding connection with the surface of the toothed plate 7 and its stable fixation between its back and the front of the imaging device body 1, constructs a precise constraint boundary. It strictly limits the displacement of the toothed plate 7 in the non-moving direction, effectively preventing the toothed plate 7 from swaying left and right or shifting up and down during movement.

[0064] Reference Figure 6 As shown in this embodiment: the top of the limiting block 29 is provided with a through hole 30, the front of the connecting rod 8 passes through the front of the limiting block 29, and a round block 31 is fixedly connected to the front of the connecting rod 8.

[0065] Specifically, the through hole 30 can be used to limit the gear 61 in conjunction with the round block 31 and the connecting rod 8, preventing the gear 61 from shaking and becoming unstable during long-term rotation.

[0066] Reference Figure 5 As shown in this embodiment: a rubber pad 32 is fixedly connected to the outer side of the telescopic block 19, and mounting ears 33 are fixedly connected to both the front and back sides of the toothed plate 7. The mounting ears 33 are used to reinforce the fixation between the carrier 3 and the toothed plate 7.

[0067] Specifically: By setting the rubber pad 32, the service life of the carrier 3 and the telescopic block 19 can be increased. By setting the mounting ear 33, the mounting ear 33 can be used to reinforce the fixation between the carrier 3 and the toothed plate 7.

[0068] In practice, gear 61 and toothed plate 7 cooperate with each other. When gear 61 rotates, it moves along the tooth groove of toothed plate 7. The movement of toothed plate 7 directly drives the carrier 3 to move smoothly on the preset track. When the carrier 3 moves to the appropriate position (see Embodiment 1), the servo motor 9 is stopped in time. After the initial positioning of the carrier 3 is completed, it needs to be reliably supported to ensure stability in subsequent use. The torque characteristics of the reset torsion spring 23 play a role. The reset torsion spring 23 stores a certain amount of elastic potential energy in advance, which is released when needed, causing the hollow block 18 to rotate on the surface of the crossbar 17. During the rotation of the hollow block 18, it will drive the telescopic block 19 connected to it to rotate synchronously. The user removes the positioning bolt 21 to release the original position restriction of the telescopic block 19, making it adjustable. According to the actual height of the carrier 3, the user flexibly adjusts the position of the telescopic block 19. After the telescopic block 19 is adjusted to the appropriate height, the positioning bolt 21 is reinserted to limit the position. The position is fixed. Secondly, during the process of the connecting rod 8 rotating and driving the gear 61 rack and pinion to move the carrier 3, it will also drive the cleaning mechanism to work through the transmission device. When the connecting rod 8 rotates, it will drive the drive pulley 11 connected to it to rotate. The drive pulley 11 transmits power to the rotating pulley 12 through the transmission belt, so that the rotating pulley 12 rotates synchronously. The rotating pulley 12 further drives the connecting block 13 to rotate. The connecting block 13 is connected to the cleaning block 15, thereby driving the cleaning block 15 to make a circular motion. During the rotation, the cleaning block 15 will thoroughly clean the dustproof net 5 on the surface of the ventilation port 4, removing the dust, debris and other objects attached to the dustproof net 5. Because the ventilation port 4 is designed with an inclined shape, when the cleaning block 15 cleans the dust off the dustproof net 5, the dust will slide down along the inclined surface of the ventilation port 4 and will not enter the imaging device body 1 in the opposite direction. This effectively avoids the damage of dust to the precision components inside the imaging device body 1, ensuring the normal operation and service life of the equipment.

[0069] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. A fluorescence imaging device capable of automatic distance adjustment, comprising an imaging device body (1), a base (2), and a carrier (3) disposed on top of the base (2), wherein the bottom of the imaging device body (1) is fixedly connected to the base (2), the carrier (3) is used to place a sample tube to be detected for fluorescence, and the imaging lens (0) of the imaging device body (1) faces downward toward the carrier (3) to acquire the fluorescence signal corresponding to the sample tube, and the signal is processed by a microprocessor in the imaging device body (1), characterized in that, The carrier (3) is connected to an adjustment assembly (6) for driving its movement. The adjustment assembly (6) includes a toothed plate (7) fixedly connected to the carrier (3), a gear (61) meshing with the toothed plate (7), and a servo motor (9) driving the gear (61) to rotate. The output end of the servo motor (9) is fixedly connected to the gear (61) through a connecting rod (8). The microprocessor is connected to the servo motor (9) in communication. Based on the signal-to-noise ratio of the fluorescent signal acquired in real time, the microprocessor instructs the servo motor (9) to rotate, thereby realizing the height adjustment of the vehicle (3). When the signal-to-noise ratio reaches a preset threshold, the microprocessor instructs the servo motor (9) to stop rotating.

2. The fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, A distance sensor is also provided on one side of the imaging lens (0). The distance sensor is used to obtain the distance information between the imaging lens (0) and the sample tube on the carrier (3). The distance sensor is communicatively connected to the microprocessor.

3. The fluorescence imaging device capable of automatic distance adjustment according to claim 2, characterized in that, The microprocessor instructs the servo motor (9) to rotate based on the preset target height information of the vehicle or the real-time distance between the vehicle (3) and the imaging lens fed back by the distance sensor, thereby realizing the height adjustment of the vehicle (3). When the vehicle (3) reaches the target height or the real-time distance reaches the preset focal length value range, the microprocessor instructs the servo motor (9) to rotate based on the real-time signal-to-noise ratio of the fluorescence signal, thereby realizing the height adjustment of the vehicle (3). When the signal-to-noise ratio reaches the preset threshold, the microprocessor instructs the servo motor (9) to stop rotating.

4. The fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, The carrier (3) includes at least one trough (35) for placing sample tubes.

5. A fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, The vehicle (3) has a guide hole in the middle, and the imaging device body (1) has a guide post (34) that matches the guide hole. The guide post (34) is arranged in the vertical direction and is used to guide the vehicle (3) when it is vertically displaced.

6. The fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, The microprocessor is a single-chip microcomputer or a processor based on x86, ARM, or RISC-V architecture.

7. A fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, The sample tube to be detected in the carrier (3) is a transparent glass or plastic tube, and the sample in the sample tube to be detected is a sample with a pre-added fluorescent labeling substance.

8. A fluorescence imaging device capable of automatic distance adjustment according to claim 4, characterized in that, The inner wall of the tank (35) is matte. The tank (35) is provided with at least one set of limiting columns that can match sample tubes of various sizes. Each set of limiting columns includes four flexible silicone limiting columns. When the sample tube is placed horizontally in the tank (35), the sample tube is limited by the four flexible silicone limiting columns.

9. A fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, The side of the toothed plate (7) without teeth is fixedly connected to the side of the carrier (3).

10. A fluorescence imaging device capable of automatic distance adjustment according to claim 1, characterized in that, The main body of the servo motor (9) is connected to the main body of the imaging device (1), and the output shaft of the servo motor (9) is connected to the middle part of the gear (61).

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