Triple high immunity immunoglobulin for anti swine pest, aujeszkys disease of pig, and parvovirus of pig

Abstract

An immunoglobulin for preventing the cholera, pseudorabies and parvovirus of hog is prepared from their immunogens through proportional mixing them together and proportionally mixing it with complete Frennd's adjuvant. It features that the glycoside-peptide injection is used as its immunopotentiator.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to an immunoglobulin against swine fever, porcine pseudorabies and porcine parvovirus prepared by using three immunogens. The international patent classification number is A61K39 / 395. Background technique

[0002] Classical swine fever (CSF, Hog chorea HC) is a devastating infectious disease of pigs, also known as hog cholera in the early years, is a severe infectious disease caused by swine fever virus. Since it was first reported in Tennessee, USA in 1810, it has become a global epidemic. In recent years, except for North America, Oceania and a few countries in Europe, it is still the biggest threat to the pig industry and the most important infectious disease of pigs at present. The International Health Regulations of the International Office of Epizootics (OIE) classifies swine fever as one of the 16 severe infectious diseases of Class A research; when it passes through ...

Examples

Embodiment 1

[0044] The immunogen used in the present invention is prepared by adding complete Freund's adjuvant after concentrating the cell culture, wherein the swine fever virus is cultured with bovine testicular primary cells or PHK-21 passage cells, and the porcine pseudorabies virus is cultured with porcine kidney cells (PK-15) or bovine kidney cells (MDBK) were cultivated, porcine parvovirus was cultured with porcine kidney primary or secondary cells; after concentration, the titer of these three viruses reached 10 6～8 , the three immunogens were mixed in a volume ratio of 1:2:1.5, and then Freund's adjuvant was added in a volume ratio of 1:1 as an immunogen to immunize animals. At the same time, the glycoside injection developed by Jilin Jingxiang Animal Pharmaceutical Co., Ltd. was used as an immune enhancer, 5 ml / bottle, to increase the antibody titer.

[0045] Preparation method of the present invention:

[0046] Immunization program

[0047] The immunized animals of the prese...

Embodiment 2

[0061]The swine fever virus is cultured with bovine testis primary cells or PHK-21 subcultured cells, the porcine pseudorabies virus is cultured with porcine kidney cells (PK-15) or bovine kidney cells (MDBK), and the porcine parvovirus is cultured with porcine kidney primary or subcultured cells. Generation cell culture; the titers of these three viruses reached 10 after concentration 6～8 , the three immunogens were mixed in a volume ratio of 1.5:1:1.5, and then Freund's adjuvant was added in a volume ratio of 1:1 as an immunogen to immunize animals. At the same time, the glycoside injection developed by Jilin Jingxiang Animal Pharmaceutical Co., Ltd. was used as an immune enhancer, 5 ml / bottle, to increase the antibody titer.

[0062] The preparation method is the same as in Example 1.

technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to an immunoglobulin against swine fever, porcine pseudorabies and porcine parvovirus prepared by using three immunogens. The international patent classification number is A61K39 / 395. Background technique

[0002] Classical swine fever (CSF, Hog chorea HC) is a devastating infectious disease of pigs, also known as hog cholera in the early years, is a severe infectious disease caused by swine fever virus. Since it was first reported in Tennessee, USA in 1810, it has become a global epidemic. In recent years, except for North America, Oceania and a few countries in Europe, it is still the biggest threat to the pig industry and the most important infectious disease of pigs at present. The International Health Regulations of the International Office of Epizootics (OIE) classifies swine fever as one of the 16 severe infectious diseases of Class A research; when it passes through ...

Examples

Embodiment 1

[0044] The immunogen used in the present invention is prepared by adding complete Freund's adjuvant after concentrating the cell culture, wherein the swine fever virus is cultured with bovine testicular primary cells or PHK-21 passage cells, and the porcine pseudorabies virus is cultured with porcine kidney cells (PK-15) or bovine kidney cells (MDBK) were cultivated, porcine parvovirus was cultured with porcine kidney primary or secondary cells; after concentration, the titer of these three viruses reached 10 6～8 , the three immunogens were mixed in a volume ratio of 1:2:1.5, and then Freund's adjuvant was added in a volume ratio of 1:1 as an immunogen to immunize animals. At the same time, the glycoside injection developed by Jilin Jingxiang Animal Pharmaceutical Co., Ltd. was used as an immune enhancer, 5 ml / bottle, to increase the antibody titer.

[0045] Preparation method of the present invention:

[0046] Immunization program

[0047] The immunized animals of the prese...

Embodiment 2

[0061]The swine fever virus is cultured with bovine testis primary cells or PHK-21 subcultured cells, the porcine pseudorabies virus is cultured with porcine kidney cells (PK-15) or bovine kidney cells (MDBK), and the porcine parvovirus is cultured with porcine kidney primary or subcultured cells. Generation cell culture; the titers of these three viruses reached 10 after concentration 6～8 , the three immunogens were mixed in a volume ratio of 1.5:1:1.5, and then Freund's adjuvant was added in a volume ratio of 1:1 as an immunogen to immunize animals. At the same time, the glycoside injection developed by Jilin Jingxiang Animal Pharmaceutical Co., Ltd. was used as an immune enhancer, 5 ml / bottle, to increase the antibody titer.

[0062] The preparation method is the same as in Example 1.