Solid-phase extraction instrument

Abstract

The utility model discloses a solid-phase extraction instrument which comprises a containing groove body, an opening is formed in the top of the containing groove body, an upper cover is detachably arranged at the opening, a flexible sealing strip used for being matched with the containing groove body is arranged on the bottom face of the upper cover, and a plurality of liquid guide pipes are arranged on the upper cover. A control valve is arranged at the top of the liquid guide pipe, a liquid inlet is formed in the control valve, a water baffle is arranged on the outer ring of the upper cover, a positioning support is detachably arranged on the bottom face of the containing groove body and comprises a plurality of supporting columns, and a lower supporting plate and an upper positioning plate are arranged on the supporting columns in a liftable mode. The upper positioning plate is provided with a plurality of positioning holes used for being connected with a liquid container, the containing groove body is connected with a negative pressure pipe, and the negative pressure pipe is connected with a negative pressure pump. The device has the advantages of being convenient to operate, stable in positioning and capable of preventing leakage.

Description

Technical Field

[0001] The utility model belongs to the technical field of eluent collecting equipment, and in particular relates to a solid phase extraction instrument. Background Art

[0002] Solid phase extractor, also known as solid phase extraction device, SPE solid phase extraction device, is an instrument used in the fields of environmental science and technology and resource science and technology. The eluent is a solution obtained after exchanging resins, which is produced by the regeneration liquid flowing through saturated ions.

[0003] Selection of eluent: The eluent can be water, methanol, ethanol, acetone, and acid and alkali solutions of different concentrations. Different eluents or different concentrations of the same solvent can be selected according to the strength of the adsorption effect to roughly separate the various components. The general method is as follows: ① Wash with an appropriate amount of water to wash off monosaccharides, tannins, oligosaccharides, and polysaccharide polar substances, and use thin layer chromatography to prevent the highly polar saponins from being washed off; ② Wash with 70% ethanol, the eluent is mainly saponins, but also contains phenolic substances, sugars and a small amount of flavonoids. Experiments have shown that 30% ethanol will not wash off a large amount of flavonoid compounds; ③ Wash with 3% to 5% alkaline solution, which can wash off flavonoids, organic acids, phenolic substances and amino acids; ④ Wash with 10% acid solution, which can wash off alkaloids and amino acids; ⑤ Wash with acetone, which can wash off neutral lipophilic components.

[0004] The vacuum filtration operation is also called suction filtration, which uses a vacuum pump to reduce the pressure in the suction bottle to achieve the purpose of solid-liquid separation.

[0005] The problem with the prior art is that, during the process of collecting the eluent using the filtration equipment, it is inconvenient to position the eluent collection and measurement container, and auxiliary accessories are required to position the collection container. Moreover, under the action of the negative pressure equipment, the positioning stability of the collection container is insufficient, which can easily lead to leakage and confusion of the eluent, and the applicable container range is small. During the cleaning process of the filtration equipment, liquid leakage can easily occur, affecting the cleanliness of the site. Summary of the invention

[0006] The utility model aims to provide a solid phase extractor with the advantages of convenient operation, stable positioning and leakage prevention in view of the problems existing in the prior art.

[0007] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: a solid phase extractor, including a containing tank body, the top of the containing tank body is provided with an opening, the opening is detachably provided with an upper cover, the bottom surface of the upper cover is provided with a flexible sealing strip for cooperating with the containing tank body, a plurality of liquid guide tubes are provided on the upper cover, a control valve is provided on the top of the liquid guide tube, a liquid inlet is provided on the control valve, a water baffle is provided on the outer ring of the upper cover, a positioning bracket is detachably provided on the bottom surface of the containing tank body, the positioning bracket includes a plurality of support columns, a lower support plate and an upper positioning plate are liftably provided on the support columns, a plurality of positioning holes for connecting a liquid container are provided on the upper positioning plate, a negative pressure tube is connected to the containing tank body, and the negative pressure tube is connected to a negative pressure pump.

[0008] In the above scheme, a closed space is formed by cooperating with the receiving tank body and the upper cover, and a negative pressure space is formed under the action of the negative pressure tube and the negative pressure pump to extract the eluent in the sample at the liquid inlet. The flexible sealing strip on the bottom surface of the upper cover is used to improve the sealing effect of the closed space. The liquid inlet is used to connect the sample to be collected and filtered, and the switch of the filtration is controlled by controlling the valve. The liquid guide tube penetrates the upper cover and is used to guide the eluent extracted by the negative pressure downward into the liquid container in the receiving tank body. The water baffle on the upper cover is used to collect the liquid splashed when cleaning the liquid inlet and the liquid guide tube to prevent the liquid from flowing onto the desktop, which is convenient for cleaning. The positioning bracket is used to position the liquid container so that the liquid container and the liquid guide tube are stably docked. The bottom end of the support column is placed on the bottom surface of the receiving tank body. The lower support plate and the upper positioning plate can be adjusted up and down, and can be used for positioning a variety of liquid containers. The bottom of the liquid container is placed on the lower support plate, and the upper part of the liquid container is guided and positioned through the positioning hole.

[0009] Furthermore, the lower support plate and the upper positioning plate are both provided with guide sleeves for sliding connection with the support columns, and the guide sleeves are threadedly connected with locking screws.

[0010] By setting a guide sleeve, a sliding connection between the lower support plate and the upper positioning plate and the support column is achieved, and the lower support plate and the upper positioning plate are kept level, which is convenient for connecting and positioning the liquid container. A threaded hole is opened on the guide sleeve, and a locking screw is connected to the threaded hole. The height of the lower support plate and the upper positioning plate can be locked and unlocked by rotating the locking screw.

[0011] Furthermore, a base is provided at the bottom end of the support column, a mounting hole is provided on the bottom surface of the base, and a positioning pin for cooperating with the mounting hole is provided on the bottom surface of the accommodating groove body.

[0012] The positioning stability of the positioning bracket is ensured by plugging and matching the mounting hole of the base with the positioning shaft pin, thereby avoiding displacement of the positioning bracket during operation.

[0013] Furthermore, a drain port is provided on the water retaining plate, and a drain pipe is connected to the drain port.

[0014] By opening a drain port, the water within the range of the upper cover water retaining plate can be introduced into the drain pipe for discharge, which is convenient for centralized treatment of waste liquid.

[0015] Furthermore, a mounting groove for connecting to the accommodating groove body is provided on the bottom surface of the upper cover, and the flexible sealing strip is glued to the bottom surface of the mounting groove.

[0016] A flexible sealing strip is connected in the installation groove to facilitate connection with the receiving groove body and ensure the sealing of the connection.

[0017] Furthermore, the upper cover is a rectangular structure, and four corners of the bottom surface of the upper cover are provided with supporting feet.

[0018] By arranging supporting feet on the bottom surface of the upper cover, it is convenient to position the upper cover when it is removed.

[0019] Compared with the prior art, the beneficial effects of the utility model are:

[0020] 1. A detachable positioning bracket is arranged in the receiving tank body, and the installation of the liquid container is realized by the cooperation of the lower support plate and the upper positioning plate. The positioning hole is used to position the liquid container, and the positioning operation is convenient to prevent the container from tipping over, thereby ensuring the positioning stability of the liquid container; the positioning shaft pin in the receiving tank body is plugged and connected with the base to ensure the positioning stability between the positioning bracket and the receiving tank body;

[0021] 2. By arranging the lower support plate and the upper positioning plate on the support column in a liftable manner, liquid containers of various specifications can be installed, ensuring the connection stability between the liquid container and the liquid guide tube, with wide applicability and easy use;

[0022] 3. By setting a water baffle on the upper cover, liquid is prevented from leaking onto the site when cleaning the liquid inlet and the liquid guide tube, thereby ensuring the cleanliness of the site; a drain pipe is set to facilitate the centralized discharge of waste liquid into the waste liquid tank, which is easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic structural diagram of a solid phase extraction apparatus according to Example 1 of the utility model;

[0024] Figure 2 This is a structural stereogram of a solid phase extraction apparatus according to Example 1 of the utility model;

[0025] Figure 3 This is a structural stereogram of the positioning bracket in Example 1 of the utility model;

[0026] In the figure: 1. containing tank body; 2. upper cover; 3. liquid guide tube; 4. flexible sealing strip; 5. control valve; 6. liquid inlet; 7. water baffle; 8. support column; 9. lower support plate; 10. upper positioning plate; 11. positioning hole; 12. negative pressure tube; 13. negative pressure pump; 14. guide sleeve; 15. locking screw; 16. base; 17. mounting hole; 18. positioning shaft pin; 19. drain outlet; 20. drain pipe; 21. mounting groove; 22. support foot; 23. immunoaffinity column; 24. volumetric flask. DETAILED DESCRIPTION

[0027] The technical solution of the utility model will be clearly and completely described below in conjunction with the drawings in the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative labor are within the scope of protection of the utility model. In the description of the utility model, it should be noted that the terms front, back, left, right, etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings, or the directions or positional relationships in which the utility model product is usually placed when used. They are only for the convenience of describing the utility model or simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed or operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model. Example 1

[0028] like Figure 1-3 As shown, a solid phase extractor comprises a containing tank body 1, the top of the containing tank body 1 is provided with an opening, the opening is detachably provided with an upper cover 2, the bottom surface of the upper cover 2 is provided with a flexible sealing strip 4 for cooperating with the containing tank body 1, a plurality of liquid guide tubes 3 are provided on the upper cover 2, a control valve 5 is provided on the top of the liquid guide tube 3, a liquid inlet 6 is provided on the control valve 5, a water retaining plate 7 is provided on the outer ring of the upper cover 2, a positioning bracket is detachably provided on the bottom surface of the containing tank body 1, the positioning bracket comprises a plurality of supporting columns 8, a lower supporting plate 9 and an upper positioning plate 10 are liftably provided on the supporting columns 8, a plurality of positioning holes 11 for connecting a liquid container are provided on the upper positioning plate 10, a negative pressure pipe 12 is connected to the containing tank body 1, and the negative pressure pipe 12 is connected to a negative pressure pump 13.

[0029] In the above scheme, a closed space is formed by cooperating with the receiving tank body 1 and the upper cover 2, and a negative pressure space is formed under the action of the negative pressure tube 12 and the negative pressure pump 13 to extract the eluent in the sample at the liquid inlet 6. The flexible sealing strip 4 on the bottom surface of the upper cover 2 is used to improve the sealing effect of the closed space. The liquid inlet 6 is used to connect the sample to be collected and filtered. The switch of the filtration is controlled by controlling the valve 5. The liquid guide tube 3 penetrates the upper cover 2 and is used to guide the eluent extracted by the negative pressure downward into the liquid container in the receiving tank body 1. The water baffle 7 on the upper cover 2 is used to collect the liquid splashed when cleaning the liquid inlet 6 and the liquid guide tube 3 to prevent the liquid from flowing onto the desktop and facilitate cleaning. The positioning bracket is used to position the liquid container so that the liquid container and the liquid guide tube 3 are stably docked. The bottom end of the support column 8 is placed on the bottom surface of the receiving tank body 1. The lower support plate 9 and the upper positioning plate 10 can be adjusted up and down, and can be used for positioning a variety of liquid containers. The bottom of the liquid container is placed on the lower support plate 9, and the upper part of the liquid container passes through the positioning hole 11 for guiding and positioning.

[0030] The positioning bracket includes four support columns 8. The cross sections of the receiving tank body 1, the lower support plate 9 and the upper positioning plate 10 are all rectangular. The positioning holes 11 can include various sizes and shapes.

[0031] An immunoaffinity column 23 is detachably provided at the liquid inlet 6 , and the liquid container includes a volumetric flask 24 .

[0032] Furthermore, the lower support plate 9 and the upper positioning plate 10 are both provided with guide sleeves 14 for sliding connection with the support column 8 , and the guide sleeves 14 are threadedly connected with locking screws 15 .

[0033] By setting the guide sleeve 14, a sliding connection between the lower support plate 9 and the upper positioning plate 10 and the support column 8 is achieved, and the lower support plate 9 and the upper positioning plate 10 are kept level, which is convenient for connecting and positioning the liquid container. A threaded hole is opened on the guide sleeve 14, and a locking screw 15 is connected to the threaded hole. The height locking and unlocking of the lower support plate 9 and the upper positioning plate 10 can be achieved by rotating the locking screw 15.

[0034] Furthermore, a base 16 is provided at the bottom end of the support column 8 , a mounting hole 17 is provided on the bottom surface of the base 16 , and a positioning pin 18 for cooperating with the mounting hole 17 is provided on the bottom surface of the accommodating tank body 1 .

[0035] The mounting hole 17 of the base 16 and the positioning shaft pin 18 are plugged in and matched to ensure the positioning stability of the positioning bracket and avoid displacement of the positioning bracket during operation.

[0036] Furthermore, a drain port 19 is formed on the water retaining plate 7 , and a drain pipe 20 is connected to the drain port 19 .

[0037] By providing a drain port 19, the water within the water retaining plate 7 of the upper cover 2 is introduced into the drain pipe 20 for discharge, so as to facilitate the centralized treatment of waste liquid.

[0038] The drain pipe 20 is a hose, and a waste liquid tank is provided at the outlet of the drain pipe 20 .

[0039] Furthermore, a mounting groove 21 for connecting and accommodating the tank body 1 is provided on the bottom surface of the upper cover 2 , and the flexible sealing strip 4 is glued to the bottom surface of the mounting groove 21 .

[0040] A flexible sealing strip 4 is connected in the installation groove 21 to facilitate connection with the receiving groove body 1 and ensure the sealing of the connection.

[0041] Furthermore, the upper cover 2 is a rectangular structure, and four corners of the bottom surface of the upper cover 2 are provided with supporting feet 22 .

[0042] By providing support feet 22 on the bottom surface of the upper cover 2, it is convenient to position the upper cover 2 when it is removed.

[0043] The bottom end height of the support leg 22 is lower than the bottom end height of the catheter 3 .

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A solid phase extraction apparatus, characterized in that, The invention comprises a accommodating tank body, the top of which is provided with an opening, the opening being detachably provided with an upper cover, the bottom surface of which is provided with a flexible sealing strip for cooperating with the accommodating tank body, a plurality of liquid guide tubes being provided on the upper cover, the top of which is provided with a control valve, the control valve being provided with a liquid inlet, a water baffle being provided on the outer ring of the upper cover, a detachable positioning bracket being provided on the bottom surface of the accommodating tank body, the positioning bracket comprising a plurality of supporting columns, a lower supporting plate and an upper positioning plate being liftably provided on the supporting columns, a plurality of positioning holes for connecting a liquid container being provided on the upper positioning plate, a negative pressure tube being connected to the accommodating tank body, and the negative pressure tube being connected to a negative pressure pump.

2. The solid phase extractor according to claim 1, characterized in that: The lower support plate and the upper positioning plate are both provided with guide sleeves for sliding connection with the support columns, and the guide sleeves are threadedly connected with locking screws.

3. The solid phase extractor according to claim 1, characterized in that: A base is arranged at the bottom end of the support column, a mounting hole is arranged on the bottom surface of the base, and a positioning shaft pin for cooperating with the mounting hole is arranged on the bottom surface of the accommodating groove body.

4. The solid phase extractor according to claim 1, characterized in that: The water retaining plate is provided with a drain port, and the drain port is connected with a drain pipe.

5. The solid phase extractor according to claim 1, characterized in that: The bottom surface of the upper cover is provided with a mounting groove for connecting with the accommodating groove body, and the flexible sealing strip is glued to the bottom surface of the mounting groove.

6. The solid phase extractor according to claim 1, characterized in that: The upper cover is a rectangular structure, and four corners of the bottom surface of the upper cover are provided with supporting feet.

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