Auxiliary device for intravitreal injection of rats / mice

Abstract

A rat / mouse vitreous cavity injection auxiliary device comprises a handheld compression part and two eyeball fixing parts, the handheld compression part is in a V shape, the two eyeball fixing parts are arranged at the two ends of the handheld compression part respectively, the outer side of the handheld compression part and the outer side of the eyeball fixing parts are wrapped by an elastic pipe, and the elastic pipe is connected with the handheld compression part. The side walls of the two sides of the elastic tube are each provided with at least one opening, the openings in the two sides of the elastic tube are opposite, and the middle of the elastic tube is connected with an injection tube. The handheld compression part and the eyeball fixing part enable the device to fully expose and fix eyeballs of a rat / mouse, so that the operation stability is improved; ofloxacin eye drops can be continuously and stably injected through the needle tube via the valve, so that the ocular surface and cornea of a rat / mouse are kept moist; the material is light, has certain corrosion resistance, and is convenient to operate, clean and store. According to the device, the stability of intravitreal injection operation of rats / mice is greatly improved.

Description

Technical Field

[0001] The utility model relates to the field of chemistry, in particular to experimental equipment, in particular to an auxiliary device for vitreous cavity injection of mice and rats. Background Art

[0002] Animal research on mice and rats is a common and important research method. In ophthalmology research, intravitreal injection of mice and rats is a commonly used technical means. Researchers can quickly inject various drugs into the vitreous cavity to quickly exert their effects on the eyeball. In the intravitreal injection experiment of mice and rats, the operator anesthetizes the mice and dilates the pupils, then places them under a stereo microscope. One hand is used to expand the eyelids of the mice and rats to completely expose the eyeballs, and the other hand is used to hold a microsyringe to inject drugs slightly deeper along the limbus of the cornea. This technology has certain operational difficulties: first, it is difficult to completely expose and fix the eyeballs of mice and rats with bare hands, and they are easy to shake and unstable; second, the cornea of ​​mice and rats is very easy to turn white after being anesthetized. In order to make the fundus easier to observe, researchers need to apply drug gel-ofloxacin eye drops on the surface of the eyeball many times. The above operational difficulties make the intravitreal injection experiment of mice and rats have certain operational instability and are easy to damage the internal structure of the eyeball and the normal function of the cornea. Summary of the invention

[0003] The utility model aims to provide an auxiliary device for intravitreal injection of rats and mice, and the auxiliary device for intravitreal injection of rats and mice is intended to solve the technical problem of low stability of intravitreal injection operation in the prior art.

[0004] The utility model discloses a vitreous cavity injection auxiliary device for mice and rats, comprising a handheld compression part and two eyeball fixing parts, wherein the handheld compression part is V-shaped, and the two eyeball fixing parts are respectively arranged at two ends of the handheld compression part, the outer sides of the handheld compression part and the eyeball fixing part are wrapped by an elastic tube, and the side walls on both sides of the elastic tube are respectively provided with at least one opening, and the openings on both sides of the elastic tube are facing each other, and an injection tube is connected to the middle part of the elastic tube, the inner cavity of the injection tube is communicated with the inner cavity of the elastic tube, and the upper end of the injection tube is connected to the injection device through a needle tube adapter.

[0005] Furthermore, the handheld compression part includes a metal wire made of aluminum alloy.

[0006] Furthermore, the eyeball fixing part includes two metal sheets made of titanium alloy respectively welded to the two ends of the metal wire.

[0007] Furthermore, a one-way valve is provided in the injection tube, and the flow direction of the one-way valve is from the injection tube to the elastic tube.

[0008] Furthermore, the elastic tube is a latex tube.

[0009] Compared with the prior art, the utility model has a positive and obvious effect. The hand-held compression part and the eyeball fixing part of the utility model enable the device to fully expose and fix the eyeball of mice and rats, increasing the stability of the operation; ofloxacin eye drops can be continuously and stably injected through a needle tube via a valve to keep the eye surface and cornea of ​​mice and rats moist; the material is light and has a certain degree of corrosion resistance, which is easy to operate, clean and store. In summary, the device greatly increases the stability of the vitreous cavity injection operation of mice and rats. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 The utility model is a stereoscopic schematic diagram of a vitreous cavity injection auxiliary device for rats and mice.

[0011] Figure 2 The utility model is a schematic top view of a vitreous cavity injection auxiliary device for rats and mice.

[0012] Figure 3 It is a schematic diagram of the radial cross section of the eyeball fixing part in the vitreous cavity injection auxiliary device for rats and mice of the utility model. DETAILED DESCRIPTION

[0013] The present invention is further described below in conjunction with an embodiment, but the present invention is not limited to the embodiment. Any similar structure and similar changes of the present invention should be included in the protection scope of the present invention. The use of directions such as up, down, front, back, left, and right in the present invention is only for the convenience of clear description and is not a limitation on the technical solution of the present invention.

[0014] like Figure 1-Figure 3 As shown, the utility model is a vitreous cavity injection auxiliary device for rats and mice, comprising a handheld compression part 1 and two eyeball fixing parts 2, the handheld compression part 1 is V-shaped, the two eyeball fixing parts 2 are respectively arranged at the two ends of the handheld compression part 1, the outer sides of the handheld compression part 1 and the eyeball fixing part 2 are wrapped by an elastic tube 8, the side walls on both sides of the elastic tube 8 are respectively provided with at least one opening 5, the openings 5 ​​on both sides of the elastic tube 8 are facing each other, the middle part of the elastic tube 8 is connected with an injection tube 9, the inner cavity of the injection tube 9 is connected with the inner cavity of the elastic tube 8, and the upper end of the injection tube 9 is connected with an injection device 10 through a needle tube adapter 3.

[0015] A needle tube adapter 3 (also called a needle tube connector or adapter) is a medical device used to connect a syringe, an infusion tube or other medical device to a needle tube.

[0016] Furthermore, the hand-held compression part 1 includes a metal wire 6 made of aluminum alloy. Aluminum alloy is an alloy metal with a certain amount of other alloying elements added to aluminum. It has high plasticity and can be plastically deformed by the operator's hand at room temperature. Aluminum alloy forms a dense and uniform oxide film in the air, which has a certain corrosion resistance. Aluminum alloy has the advantage of being lightweight and convenient for the operator to hold and operate.

[0017] Furthermore, the eyeball fixing part 2 includes two metal sheets 7 made of titanium alloy respectively welded to the two ends of the metal wire 6. Titanium alloy is an alloy metal made of titanium and other metals, and has the advantages of high strength, good corrosion resistance, light weight, and easy cleaning, which is convenient for operators to fix the eyeballs of rats and mice, and clean and preserve the device after the experiment.

[0018] Furthermore, a one-way valve 4 is provided in the injection tube 9 , and the flow direction of the one-way valve 4 is from the injection tube 9 to the elastic tube 8 .

[0019] Furthermore, the elastic tube 8 is a latex tube. The latex tube is made of rubber material, has the advantages of high elasticity and high tensile strength, ensures the durability of the device, and has certain biocompatibility to prevent eye allergies in rats and mice.

[0020] The aluminum alloy wire 6 has a diameter of 0.4 cm and a length of 20 cm. The titanium alloy metal sheet 7 has a length of 4 cm and a width of 0.8 cm. The latex tube has a diameter of 1 cm and is bonded to the hand-held compression part 1 by strong glue. The number of openings 5 ​​is 4, and the specification is 0.4 cm in length and 0.4 cm in width.

[0021] The needle tube adapter 3 and the one-way valve 4 are both made of polystyrene. Polystyrene is a thermoplastic synthesized from styrene monomers through free radical addition polymerization, and has the characteristics of being lightweight, colorless and transparent.

[0022] Specifically, the needle tube adapter 3, elastic tube 8, one-way valve 4, metal wire 6, metal sheet 7, etc. in this embodiment all adopt well-known solutions in the prior art, which are well understood by those skilled in the art and will not be described in detail here.

[0023] The working principle of this embodiment:

[0024] The eyeball fixing part 2 has a limiting effect, which helps the eyeball of living rats and mice to be fixed on the device. The hand-held compression part 1 composed of aluminum alloy wire 6 and latex tube has certain plasticity, and the operator can control the angle of the wire 6 on both sides by external force at room temperature, so that the eyeball of living rats and mice is fully exposed. The ofloxacin eye drops can be continuously injected by the injection device 10, and the injection of the ofloxacin eye drops can be observed by the needle tube adapter 3 and the one-way valve 4, so as to replenish at any time. The one-way valve 4 can prevent the ofloxacin eye drops from overflowing in reverse.

[0025] When the utility model is used for the intravitreal injection experiment of mice, the anesthetized mice are placed under a stereo microscope, and the operator places the device on both sides of the mouse's eyeball, and fully exposes and fixes the mouse's eyeball by moving and compressing the hand-held compression part 1. Then the operator continuously injects ofloxacin eye drops through the injection device 10, which flows out through the openings 5 ​​of the latex tubes on both sides and enters the eye, so as to keep the mouse's eye surface moist and ensure the normal function of the cornea. Then, in this state, the operator performs the intravitreal injection operation of the mouse.

[0026] It should be noted that before using the device, physiological saline can be injected through the injection device 10 to test the air tightness of the device; after completing the vitreous cavity injection operation of rats and mice, the device needs to be cleaned in time. The latex tube, polystyrene needle adapter 3, and polystyrene one-way valve 4 are not resistant to high temperatures, so this device needs to be kept away from exposure to the sun and fire.

[0027] The hand-held compression part 1 and the eyeball fixing part 2 of the utility model realize the full exposure and fixation of the eyeball of mice and rats by the device, and increase the stability of operation; ofloxacin eye drops can be continuously and stably injected through the needle tube via the valve to keep the eye surface and cornea of ​​mice and rats moist; the material is light and has a certain corrosion resistance, which is convenient for operation, cleaning and storage. In summary, the device greatly increases the stability of the vitreous cavity injection operation of mice and rats.

Claims

1. A vitreous cavity injection assisting device for rats and mice, characterized in that: The invention comprises a hand-held compression part and two eyeball fixing parts, wherein the hand-held compression part is V-shaped, and the two eyeball fixing parts are respectively arranged at two ends of the hand-held compression part, and the outer sides of the hand-held compression part and the eyeball fixing part are wrapped by an elastic tube, and the side walls on both sides of the elastic tube are respectively provided with at least one opening, and the openings on both sides of the elastic tube are facing each other, and an injection tube is connected to the middle part of the elastic tube, and the inner cavity of the injection tube is communicated with the inner cavity of the elastic tube, and the upper end of the injection tube is connected to the injection device through a needle tube adapter.

2. The vitreous cavity injection assisting device for rats and mice according to claim 1, characterized in that: The hand-held compression portion includes a metal wire composed of an aluminum alloy.

3. The vitreous cavity injection assisting device for rats and mice according to claim 1, characterized in that: The eyeball fixing part comprises two metal sheets made of titanium alloy which are respectively welded to two ends of the metal wire.

4. The vitreous cavity injection assisting device for rats and mice according to claim 1, characterized in that: The injection tube is provided with a one-way valve, and the flow direction of the one-way valve is from the injection tube to the elastic tube.

5. The vitreous cavity injection assisting device for rats and mice according to claim 1, characterized in that: The elastic tube is a latex tube.

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