Centrifugal device for protein purification

By designing a centrifugal device with a water bath cavity, filter plate and stable components, the problem of low protein purification efficiency caused by too low temperature is solved, and protein purification is achieved at the appropriate temperature, reducing noise and improving centrifugal stability.

CN222138994UActive Publication Date: 2024-12-10上海信裕生物科技有限公司
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Patent Information

Application Number
CN202420648224.2
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-01
Publication Date
2024-12-10
Estimated Expiration
2034-04-01

AI Technical Summary

Technical Problem

The existing centrifugal devices have too low temperature during centrifugation, resulting in high viscosity of raw materials, affecting the efficiency of protein purification.

Method used

A centrifugal device including an outer guard barrel and an auxiliary component is designed. The auxiliary component includes a water bath cavity, a filter plate, a control rod, a cleaning hole and a top plate. Through the water liquid temperature adjustment inside the water bath cavity, vertical installation of the filter plate and cleaning holes. The design ensures that the centrifugal barrel is purified at the appropriate temperature, and reduces noise through the packaging ring, sealing groove and sound insulation cover, and increases the rotational stability of the centrifugal barrel.

Benefits of technology

Protein purification is achieved at a suitable temperature, avoiding impurity deposition, reducing noise, and improving the rotational stability of the centrifugal barrel, thereby improving the efficiency of protein purification.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a centrifugal device for protein purification, which relates to the technical field of protein purification and comprises an outer protective barrel and an auxiliary component, a centrifugal barrel is arranged in the outer protective barrel, and the auxiliary component for providing temperature change is arranged on the inner sides of the outer protective barrel and the centrifugal barrel. The auxiliary assembly comprises a water bath cavity, a filter plate, a control rod, a cleaning hole and a top plate, the filter plate is vertically mounted in the water bath cavity, the control rod is mounted at the top of the filter plate, the cleaning hole is formed in the exterior of the control rod, and the top plate is arranged outside the cleaning hole. According to the centrifugal device for protein purification, the temperature of the centrifugal barrel can be adjusted by adjusting the temperature of water in the water bath cavity, so that the centrifugal barrel is located at a proper temperature when protein is purified, the two groups of vertical filter plates can play a role in blocking and filtering impurities in the water in the water bath cavity, and the impurities are prevented from sinking to the bottom and being accumulated; and the cleaning holes are used for removing and cleaning the filter plate.
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Description

Technical Field

[0001] The utility model relates to the technical field of protein purification, in particular to a centrifugal device for protein purification. Background Art

[0002] Biological protein, also known as biologically active protein and active protein peptide, has a variety of human metabolic and physiological regulatory functions, is easy to digest and absorb, and has the effects of promoting immunity, hormone regulation, antibacterial, antiviral, lowering blood pressure, lowering blood lipids, etc. It is extremely safe for consumption. It is the hottest research topic in the current international food industry and a functional factor with great development prospects. When preparing biological protein, it needs to be purified, and most of them are processed by centrifugation.

[0003] However, when the existing centrifugal device is centrifuged, the temperature is too low, which will cause the viscosity of the raw materials to be high and affect the efficiency of purification.

[0004] Therefore, in view of this, the existing structure and defects are studied and improved, and a centrifugal device for protein purification is provided. Utility Model Content

[0005] The utility model aims to provide a centrifugal device for protein purification to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a centrifugal device for protein purification, comprising an outer protective barrel and an auxiliary component, wherein a centrifugal barrel is arranged inside the outer protective barrel, and the auxiliary component for providing temperature change is arranged on the inner side of the outer protective barrel and the centrifugal barrel, and the auxiliary component comprises a water bath chamber, a filter plate, a control rod, a cleaning hole and a top plate, wherein a filter plate is vertically installed inside the water bath chamber, and a control rod is installed on the top of the filter plate, a cleaning hole is arranged outside the control rod, and a top plate is arranged outside the cleaning hole.

[0007] Furthermore, a sealing ring is installed on the outer surface of the outer protective barrel, and a sealing groove is opened on the surface of the sealing ring.

[0008] Furthermore, a reinforcement column is installed at the lower end of the packaging ring, and a power box is installed at the lower end of the reinforcement column.

[0009] Furthermore, a soundproof cover is installed inside the sealing groove, and a sealing sheet is installed on the surface of the soundproof cover.

[0010] Furthermore, a placement table is provided inside the centrifugal barrel, and a placement hole is opened on the surface of the placement table.

[0011] Furthermore, a stabilizing component for increasing centrifugal stability is provided at the lower end of the placement table, and the placement table and the placement hole are an integrated structure.

[0012] Furthermore, the stabilizing assembly includes a stabilizing box and a movable groove, and the movable groove is provided on the surface of the stabilizing box.

[0013] Furthermore, the stabilizing assembly also includes a movable bead and a connecting rod. The movable bead is arranged inside the movable groove, and the connecting rod is installed on the upper end of the movable bead.

[0014] The utility model provides a centrifugal device for protein purification, which has the following beneficial effects:

[0015] 1. The utility model can adjust the temperature of the centrifugal barrel by adjusting the temperature of the water in the water bath chamber, so that the centrifugal barrel is at a suitable temperature when purifying the protein. The two groups of vertical filter plates can filter the impurities in the water in the water bath chamber to avoid the accumulation of impurities at the bottom. The cleaning holes are used for removing and cleaning the filter plates. The top plate cooperates with the outer protective barrel to protect the centrifugal barrel.

[0016] 2. The utility model is used for the installation of a soundproof cover through a packaging ring and a sealing groove. The soundproof cover has a soundproofing effect on the noise during centrifugal processing. The movable groove in the stabilizing box is used for limiting the movable bead, so that the connecting rod and the movable bead increase the rotation stability of the centrifugal barrel when the centrifugal barrel rotates. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall structure of a centrifugal device for protein purification according to the utility model;

[0018] Figure 2 This is a schematic diagram of the auxiliary component structure of a centrifugal device for protein purification according to the utility model;

[0019] Figure 3 The utility model is a schematic diagram of the structure of a stable component of a centrifugal device for protein purification.

[0020] In the figure: 1. outer protective barrel; 2. packaging ring; 3. sealing groove; 4. reinforcement column; 5. power box; 6. sound insulation cover; 7. sealing plate; 8. centrifugal barrel; 9. auxiliary component; 901. water bath chamber; 902. filter plate; 903. control rod; 904. cleaning hole; 905. top plate; 10. stabilizing component; 1001. stabilizing box; 1002. movable groove; 1003. movable bead; 1004. connecting rod; 11. placement table; 12. placement hole. DETAILED DESCRIPTION

[0021] The following is a further detailed description of the implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0022] like Figure 1As shown, a centrifugal device for protein purification includes an outer protective barrel 1 and an auxiliary component 9. A centrifugal barrel 8 is arranged inside the outer protective barrel 1. A packaging ring 2 is installed on the outer surface of the outer protective barrel 1. The packaging ring 2 and the sealing groove 3 are used for the installation of a sound insulation cover 6. The sealing groove 3 is opened on the surface of the packaging ring 2. A reinforcement column 4 is installed at the lower end of the packaging ring 2, and a power box 5 is installed at the lower end of the reinforcement column 4. A sound insulation cover 6 is installed inside the sealing groove 3. The sound insulation cover 6 has a sound insulation effect on the noise during centrifugal treatment, and a sealing sheet 7 is installed on the surface of the sound insulation cover 6.

[0023] like Figure 2 As shown, the auxiliary component 9 for providing temperature change is arranged on the inner side of the outer protective barrel 1 and the centrifugal barrel 8, and the auxiliary component 9 includes a water bath chamber 901, a filter plate 902, a control rod 903, a cleaning hole 904 and a top plate 905. The filter plate 902 is vertically installed inside the water bath chamber 901. The temperature of the water in the water bath chamber 901 can be adjusted to adjust the temperature of the centrifugal barrel 8, so that the centrifugal barrel 8 is at a suitable temperature when purifying the protein, and the control rod 903 is installed on the top of the filter plate 902. The two groups of vertical filter plates 902 can filter the impurities in the water bath chamber 901 to prevent the impurities from settling and accumulating. A cleaning hole 904 is arranged on the outside of the control rod 903. The cleaning hole 904 is used for removing and cleaning the filter plate 902. The top plate 905 cooperates with the outer protective barrel 1 to protect the centrifugal barrel 8. The top plate 905 is arranged on the outside of the cleaning hole 904. A placing table 11 is arranged inside the centrifugal barrel 8, and a placing hole 12 is opened on the surface of the placing table 11.

[0024] like Figure 3 As shown, a stabilizing component 10 for increasing centrifugal stability is provided at the lower end of the placement table 11, and the placement table 11 and the placement hole 12 are an integrated structure, the stabilizing component 10 includes a stabilizing box 1001 and a movable groove 1002, and the surface of the stabilizing box 1001 is provided with a movable groove 1002, and the movable groove 1002 in the stabilizing box 1001 is used for limiting the movement of the movable bead 1003, the stabilizing component 10 also includes the movable bead 1003 and a connecting rod 1004, the movable bead 1003 is provided inside the movable groove 1002, and the connecting rod 1004 is installed on the upper end of the movable bead 1003, so that the connecting rod 1004 and the movable bead 1003 increase the rotation stability of the centrifugal bucket 8 when the centrifugal bucket 8 rotates.

[0025] In summary, the centrifugal device used for protein purification is firstly based on Figure 1-Figure 3The structure shown in the figure is to place the protein reagent tube to be centrifuged in the placement hole 12 in the placement table 11, and the sound insulation cover 6 is installed in the packaging ring 2, so that the noise can be reduced during the subsequent centrifugation process, and the appropriate temperature for this protein purification is adjusted, and the water is transported to the inside of the water bath chamber 901, so that the centrifuge bucket 8 is at a suitable temperature when purifying the protein. At the same time, two groups of vertical filter plates 902 can play a role in blocking and filtering impurities for the water flowing in the water bath chamber 901, avoiding the accumulation of impurities at the bottom. When cleaning, hold the control rod 903 to take out the filter plate 902. During centrifugation, the movable groove 1002 in the stabilizing box 1001 is used for the limiting activity of the movable bead 1003, so that the connecting rod 1004 and the movable bead 1003 increase the rotation stability of the centrifuge bucket 8 when the centrifuge bucket 8 rotates.

[0026] The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

Claims

1. A centrifugal device for protein purification, comprising an outer protective barrel (1) and an auxiliary component (9), characterized in that: A centrifugal barrel (8) is arranged inside the outer protective barrel (1), and the auxiliary component (9) for providing temperature change is arranged on the inner side of the outer protective barrel (1) and the centrifugal barrel (8), and the auxiliary component (9) includes a water bath chamber (901), a filter plate (902), a control rod (903), a cleaning hole (904) and a top plate (905). The filter plate (902) is vertically installed inside the water bath chamber (901), and the control rod (903) is installed on the top of the filter plate (902), the cleaning hole (904) is arranged outside the control rod (903), and the top plate (905) is arranged outside the cleaning hole (904).

2. A centrifugal device for protein purification according to claim 1, characterized in that: A sealing ring (2) is installed on the outer surface of the outer protective barrel (1), and a sealing groove (3) is provided on the surface of the sealing ring (2).

3. A centrifugal device for protein purification according to claim 2, characterized in that: A reinforcement column (4) is installed at the lower end of the packaging ring (2), and a power box (5) is installed at the lower end of the reinforcement column (4).

4. A centrifugal device for protein purification according to claim 3, characterized in that: A soundproof cover (6) is installed inside the sealing groove (3), and a sealing sheet (7) is installed on the surface of the soundproof cover (6).

5. A centrifugal device for protein purification according to claim 1, characterized in that: A placement platform (11) is provided inside the centrifugal barrel (8), and a placement hole (12) is provided on the surface of the placement platform (11).

6. A centrifugal device for protein purification according to claim 5, characterized in that: A stabilizing component (10) for increasing centrifugal stability is provided at the lower end of the placement platform (11), and the placement platform (11) and the placement hole (12) are an integrated structure.

7. A centrifugal device for protein purification according to claim 6, characterized in that: The stabilizing assembly (10) comprises a stabilizing box (1001) and a movable groove (1002), and the movable groove (1002) is provided on the surface of the stabilizing box (1001).

8. A centrifugal device for protein purification according to claim 7, characterized in that: The stabilizing assembly (10) further comprises a movable bead (1003) and a connecting rod (1004); the movable bead (1003) is arranged inside the movable groove (1002), and the connecting rod (1004) is installed on the upper end of the movable bead (1003).