Protein purification instrument

Abstract

The utility model belongs to the technical field of protein purification instruments, and particularly relates to a protein purification instrument which comprises a main body frame, a lifting air cylinder is fixedly connected to the inner wall of the main body frame, a lifting frame is fixedly connected to the output end of the lifting air cylinder, and a telescopic air cylinder is fixedly connected to the inner wall of the lifting frame. A working pipe is fixedly connected to the output end of the telescopic air cylinder, a stirring motor is fixedly connected to the position, located on one side of the working pipe, of the inner wall of the lifting frame, a stirring rod and the working pipe are arranged, a connecting rod rotates to drive the stirring rod to stir reaction liquid in a reaction cup, and meanwhile a magnetic attraction assembly works to generate a magnetic field; when a reagent is injected into the reaction cup, the telescopic air cylinder works to drive the working pipe to vertically slide along the inner wall of the lifting frame, the working pipe is lifted along with the height of a solution in the reaction cup, and the situation that the distance between the working pipe and the solution in the reaction cup is too large, and consequently the reagent is prone to splashing is avoided.

Description

Technical Field

[0001] The utility model belongs to the technical field of protein purifiers, and in particular relates to a protein purifier. Background Art

[0002] The protein purifier is a protein purification instrument used in large life science laboratories. Compared with traditional methods, it greatly improves the efficiency of purification work and saves a lot of manpower and material resources. The protein purifier can carry out various commonly used purification techniques, such as affinity chromatography, ion exchange chromatography, hydrophobic chromatography, and gel filtration. Therefore, this instrument can support the purification of various proteins, including recombinant proteins, antibodies, vaccines or natural proteins.

[0003] For example, the patent with publication number CN219885967U discloses a protein purifier, including a reagent bottle, a waste liquid bottle, a plurality of reaction tubes, a fixed seat, an agitator, a discarded liquid suction head, a syringe pump, a discarded liquid pump and an injection head, wherein the plurality of reaction tubes are fixed on the fixed seat, the liquid inlet port of the syringe pump is connected to the reagent bottle through a liquid discharge pipe, the liquid discharge port of the syringe pump is connected to the injection head, and the injection head is used to inject the liquid in the reagent bottle into the reaction tube; the agitator is used to stir the liquid in the reaction tube; the liquid discharge port of the discarded liquid pump is connected to the waste liquid bottle through a waste liquid discharge pipe, the liquid inlet port of the discarded liquid pump is connected to the discarded liquid suction head, and the discarded liquid suction head is used to extend into the reaction tube to extract the discarded liquid. The utility model improves the purification volume through technical improvements, can meet the purification of large volumes of samples to be purified, and has the characteristics of easy use and high purification efficiency.

[0004] When the existing protein purifier is used, the injection height of the reagent is too high, which causes the reagent to splash easily. In view of this, we propose a protein purifier. Utility Model Content

[0005] The utility model aims to provide a protein purification instrument to solve the above-mentioned technical problems, so as to avoid the problem that the reagent is easily splashed due to the injection height of the reagent being too high.

[0006] In view of this, the utility model provides a protein purification instrument, including a main frame, the inner wall of the main frame is fixedly connected with a lifting cylinder, the output end of the lifting cylinder is fixedly connected with the lifting frame, the inner wall of the lifting frame is fixedly connected with a telescopic cylinder, the output end of the telescopic cylinder is fixedly connected with a working tube, the inner wall of the lifting frame is located on one side of the working tube and is fixedly connected with a stirring motor, the output end of the stirring motor is fixedly connected with a driving gear, the meshing surface of the driving gear is meshed with a driven gear disk sleeved on the outside of the working tube, the bottom end of the driven gear disk is fixedly connected with a connecting block screwed with the bottom end of the lifting frame, the outer wall of the connecting block is fixedly connected with a connecting rod, the outer wall of the connecting rod is fixedly connected with a stirring rod, and the outside of the stirring rod is located on the inner wall of the main frame and a reaction cup is provided;

[0007] The clamping assembly is located inside the main frame and is used to fix the reaction cup.

[0008] Based on the above structure, the lifting cylinder drives the working tube and the connecting rod at the bottom of the lifting frame to extend into the reaction cup, and the stirring motor drives the connecting block to rotate through the driving gear and the driven gear plate. The rotation of the connecting block drives the three groups of connecting rods to rotate synchronously, so that the rotation of the connecting rod drives the stirring rod to stir the reaction liquid in the reaction cup. At the same time, the magnetic suction component works to generate a magnetic field to separate the target protein from the mixture. When the reagent is injected into the reaction cup, the telescopic cylinder drives the working tube to slide vertically along the inner wall of the lifting frame. The working tube is lifted with the height of the solution in the reaction cup to avoid the distance between the working tube and the solution in the reaction cup being too high, which makes the reagent easy to splash.

[0009] Preferably, the connecting rods are provided in three groups, and the three groups of connecting rods are distributed at equal angles around the connecting block.

[0010] Preferably, the stirring rods are provided in three groups, and the three groups of stirring rods are equidistantly distributed along the connecting rod.

[0011] Preferably, the central axis of the reaction cup coincides with the central axis of the working tube, and a liquid injection tube, a waste liquid discharge tube and a liquid discharge tube are arranged inside the working tube.

[0012] Preferably, the clamping assembly comprises:

[0013] A screw rod is provided on the inner wall of the main frame at one side of the reaction cup, a threaded slider is threadedly connected to the outer wall of the screw rod, clamping rods are screwed on both ends of the threaded slider, one end of the clamping rod is screwed on a clamping plate slidably connected to the inner wall of the main frame, a magnetic suction component is provided at the connection portion between the clamping plate and the reaction cup, and a reagent bottle is provided on the top of the main frame.

[0014] Preferably, the clamping plate forms a clamping structure with the reaction cup through the threaded slider and the clamping support rod.

[0015] Preferably, the connection portion between the magnetic attraction component and the reaction cup is arc-shaped.

[0016] The beneficial effects of the utility model are:

[0017] 1. The protein purifier is provided with a stirring rod and a working tube. The connecting rod rotates to drive the stirring rod to stir the reaction solution in the reaction cup. At the same time, the magnetic suction component works to generate a magnetic field to separate the target protein from the mixture. When the reagent is injected into the reaction cup, the telescopic cylinder works to drive the working tube to slide vertically along the inner wall of the lifting frame. The working tube is lifted with the height of the solution in the reaction cup to avoid the working tube and the solution in the reaction cup being too high, which may cause the reagent to splash easily.

[0018] 2. The protein purifier is provided with a clamping plate. The rotation of the screw drives two groups of clamping rods to move synchronously through the threaded slider, so that the threaded slider drives the two groups of clamping plates to move relative to each other through the clamping rod, which is convenient for driving the clamping plate to clamp the reaction cup and for coinciding the central axis of the reaction cup with the central axis of the working tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 It is a schematic diagram of the overall cross-sectional structure of the utility model;

[0021] Figure 3 This is a cross-sectional structural diagram of the lifting frame of the utility model;

[0022] Figure 4 It is a schematic diagram of the clamping plate connection structure of the utility model.

[0023] The symbols in the figure are:

[0024] 1. Main frame; 2. Lifting cylinder; 3. Lifting frame; 4. Telescopic cylinder; 5. Working tube; 6. Stirring motor; 7. Driving gear; 8. Driven gear plate; 9. Connecting block; 10. Connecting rod; 11. Stirring rod; 12. Reaction cup; 13. Screw; 14. Threaded slider; 15. Clamping support rod; 16. Clamping plate; 17. Magnetic suction assembly; 18. Reagent bottle. DETAILED DESCRIPTION

[0025] The following is combined with Figure 1 - Figure 4 This application is described in further detail.

[0026] In the present application, the terms "upper", "lower", "left", "right", "front", "back", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings. These terms are mainly used to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to have a specific orientation, or to be constructed and operated in a specific orientation.

[0027] The embodiment of the present application discloses a protein purification instrument, including a main frame 1, the inner wall of the main frame 1 is fixedly connected with a lifting cylinder 2, the output end of the lifting cylinder 2 is fixedly connected with a lifting frame 3, the inner wall of the lifting frame 3 is fixedly connected with a telescopic cylinder 4, the output end of the telescopic cylinder 4 is fixedly connected with a working tube 5, the inner wall of the lifting frame 3 is located on one side of the working tube 5 and is fixedly connected with a stirring motor 6, the output end of the stirring motor 6 is fixedly connected with a driving gear 7, the meshing surface of the driving gear 7 is meshed with a driven gear plate 8 sleeved on the outside of the working tube 5, the bottom end of the driven gear plate 8 is fixedly connected with a connecting block 9 that is screwed to the bottom end of the lifting frame 3, the outer wall of the connecting block 9 is fixedly connected with a connecting rod 10, the outer wall of the connecting rod 10 is fixedly connected with a stirring rod 11, and the outside of the stirring rod 11 is located on the inner wall of the main frame 1 and a reaction cup 12 is provided;

[0028] The clamping assembly is located inside the main frame 1 and is used to fix the reaction cup 12 .

[0029] Based on the above structure, the lifting cylinder 2 drives the working tube 5 and the connecting rod 10 at the bottom of the lifting frame 3 to extend into the reaction cup 12, and the stirring motor 6 drives the connecting block 9 to rotate through the driving gear 7 and the driven gear plate 8. The rotation of the connecting block 9 drives the three groups of connecting rods 10 to rotate synchronously, so that the rotation of the connecting rod 10 drives the stirring rod 11 to stir the reaction liquid in the reaction cup 12. At the same time, the magnetic suction component 17 works to generate a magnetic field to separate the target protein from the mixture. When the reagent is injected into the reaction cup 12, the telescopic cylinder 4 works to drive the working tube 5 to slide vertically along the inner wall of the lifting frame 3. The working tube 5 is lifted with the height of the solution in the reaction cup 12 to avoid the working tube 5 and the solution in the reaction cup 12. Too high a distance is avoided, which makes it easy for the reagent to splash.

[0030] In one embodiment, three groups of connecting rods 10 are provided, and the three groups of connecting rods 10 are distributed at equal angles around the connecting block 9 .

[0031] In this embodiment, by providing three groups of connecting rods 10, the connection block 9 rotates to drive the three groups of connecting rods 10 to rotate synchronously, thereby realizing the control operation of the synchronous rotation of the three groups of connecting rods 10, and greatly improving the stirring efficiency of the solution in the reaction cup 12.

[0032] In one embodiment, three groups of stirring rods 11 are provided, and the three groups of stirring rods 11 are equidistantly distributed along the connecting rod 10 .

[0033] In this embodiment, three groups of stirring rods 11 are provided, and the connecting rods 10 rotate to drive the stirring rods 11 to stir the reaction liquid in the reaction cup 12 , and to stir the solutions at different heights in the reaction cup 12 .

[0034] In one embodiment, the central axis of the reaction cup 12 coincides with the central axis of the working tube 5 , and the working tube 5 is provided with a liquid injection pipe, a waste liquid discharge pipe and a liquid discharge pipe.

[0035] In this embodiment, the telescopic cylinder 4 drives the working tube 5 to slide vertically along the inner wall of the lifting frame 3. The working tube 5 is lifted along with the height of the solution in the reaction cup 12 to avoid the working tube 5 and the solution in the reaction cup 12 being too high, which may cause the reagent to splash easily.

[0036] In one embodiment, the clamping assembly includes:

[0037] A screw 13 is provided on the inner wall of the main frame 1 on one side of the reaction cup 12, a threaded slider 14 is threadedly connected to the outer wall of the screw 13, clamping rods 15 are screwed on both ends of the threaded slider 14, one end of the clamping rod 15 is screwed on a clamping plate 16 which is slidably connected to the inner wall of the main frame 1, a magnetic suction component 17 is provided at the connection portion between the clamping plate 16 and the reaction cup 12, and a reagent bottle 18 is provided at the top of the main frame 1.

[0038] In this embodiment, the screw rod 13 rotates through the threaded slider 14 to drive the two groups of clamping rods 15 to move synchronously, so that the threaded slider 14 drives the two groups of clamping plates 16 to move relative to each other through the clamping rods 15, so as to drive the clamping plates 16 to clamp the reaction cup 12, so as to make the central axis of the reaction cup 12 coincide with the central axis of the working tube 5.

[0039] In one embodiment, the clamping plate 16 forms a clamping structure with the reaction cup 12 through the threaded slider 14 and the clamping support rod 15 .

[0040] In this embodiment, the threaded slider 14 drives the two groups of clamping plates 16 to move relative to each other through the clamping support rod 15, so as to drive the clamping plates 16 to clamp the reaction cup 12.

[0041] In one embodiment, the connection portion between the magnetic attraction component 17 and the reaction cup 12 is arc-shaped.

[0042] In this embodiment, the magnetic attraction component 17 is closely attached to the inner wall of the reaction cup 12, which is conducive to the magnetic attraction component 17 to generate a magnetic field and separate the target protein from the mixture.

[0043] When the protein purifier of this embodiment is in use, first, the experimenter puts the reaction cup 12 containing the sample into the main frame 1, and then performs a stable clamping operation on the reaction cup 12. The experimenter rotates the screw 13, and the rotation of the screw 13 drives the two groups of clamping support rods 15 to move synchronously through the threaded slider 14, so that the threaded slider 14 drives the two groups of clamping plates 16 to move relative to each other through the clamping support rod 15, so as to drive the clamping plate 16 to clamp the reaction cup 12, so as to make the central axis of the reaction cup 12 coincide with the central axis of the working tube 5.

[0044] Next, the lifting cylinder 2 drives the working tube 5 and the connecting rod 10 at the bottom of the lifting frame 3 to extend into the reaction cup 12. Then, the reagent in the reagent bottle 18 is injected into the reaction cup 12 through the injection tube inside the working tube 5 by the injection pump. Then, the stirring motor 6 drives the connecting block 9 to rotate through the driving gear 7 and the driven gear plate 8. The rotation of the connecting block 9 drives the three groups of connecting rods 10 to rotate synchronously, so that the rotation of the connecting rod 10 drives the stirring rod 11 to stir the reaction liquid in the reaction cup 12. At the same time, the magnetic suction component 17 works to generate a magnetic field to separate the target protein from the mixture.

[0045] Finally, when the reagent is injected into the reaction cup 12, the telescopic cylinder 4 drives the working tube 5 to slide vertically along the inner wall of the lifting frame 3. The working tube 5 is lifted along with the height of the solution in the reaction cup 12 to avoid the working tube 5 and the solution in the reaction cup 12 being too high, which may cause the reagent to splash easily.

[0046] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. A protein purification instrument, characterized in that: include: A main body frame (1), wherein the inner wall of the main body frame (1) is fixedly connected to a lifting cylinder (2), the output end of the lifting cylinder (2) is fixedly connected to a lifting frame (3), the inner wall of the lifting frame (3) is fixedly connected to a telescopic cylinder (4), the output end of the telescopic cylinder (4) is fixedly connected to a working tube (5), the inner wall of the lifting frame (3) is located on one side of the working tube (5) and is fixedly connected to a stirring motor (6), the output end of the stirring motor (6) is fixedly connected to a driving gear (7), the meshing surface of the driving gear (7) is meshed with a driven gear disc (8) sleeved on the outside of the working tube (5), the bottom end of the driven gear disc (8) is fixedly connected to a connecting block (9) screwed to the bottom end of the lifting frame (3), the outer wall of the connecting block (9) is fixedly connected to a connecting rod (10), the outer wall of the connecting rod (10) is fixedly connected to a stirring rod (11), and a reaction cup (12) is provided on the outside of the stirring rod (11) and located on the inner wall of the main body frame (1); A clamping assembly, the clamping assembly is located inside the main frame (1) and is used to fix the reaction cup (12).

2. The protein purification apparatus according to claim 1, characterized in that: The connecting rods (10) are provided in three groups, and the three groups of connecting rods (10) are distributed at equal angles around the connecting block (9).

3. The protein purification apparatus according to claim 1, characterized in that: The stirring rods (11) are provided in three groups, and the three groups of stirring rods (11) are distributed at equal distances along the connecting rod (10).

4. The protein purification apparatus according to claim 1, characterized in that: The central axis of the reaction cup (12) coincides with the central axis of the working tube (5), and a liquid injection pipe, a waste liquid discharge pipe and a liquid discharge pipe are arranged inside the working tube (5).

5. The protein purification apparatus according to claim 1, characterized in that: The clamping assembly comprises: A screw rod (13), the inner wall of the main frame (1) is located on one side of the reaction cup (12) and is provided with a screw rod (13), the outer wall of the screw rod (13) is threadedly connected to a threaded slider (14), both ends of the threaded slider (14) are screwed to clamping rods (15), one end of the clamping rod (15) is screwed to a clamping plate (16) slidably connected to the inner wall of the main frame (1), a magnetic attraction component (17) is provided at the connection portion between the clamping plate (16) and the reaction cup (12), and a reagent bottle (18) is provided at the top of the main frame (1).

6. The protein purification apparatus according to claim 5, characterized in that: The clamping plate (16) forms a clamping structure with the reaction cup (12) through the threaded slider (14) and the clamping support rod (15).

7. The protein purification apparatus according to claim 5, characterized in that: The connection portion between the magnetic attraction component (17) and the reaction cup (12) is arc-shaped.

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