Tissue sample chopping and sample application device

Abstract

In order to solve the problems that a tissue sample is inconvenient to shear, easy to pollute and the like, the utility model provides a tissue sample chopping and sample application device, which comprises a tissue container, a first hollow piston tube and a second hollow piston tube, the other end is positioned outside the container body; the electric shearing device and the sample application device are detachably connected with the tissue container, each sample application device is composed of a quantitative tube and a sample application tube connected to the lower portion of the quantitative tube, a sample injection hole is formed in one side of the upper portion of the quantitative tube, a connecting piece is fixedly connected to the sample injection hole, and a second hollow piston tube is slidably connected to the interior of the quantitative tube in the axial direction; one end of the piston of the second hollow piston rod is positioned inside the quantitative tube, and the other end of the piston of the second hollow piston rod is positioned outside the quantitative tube, tissue block chopping and quantitative sample application operation are performed in a closed environment, the external environment pollution probability is reduced, the cell viability is guaranteed, and the working efficiency is effectively improved.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue cell culture, in particular to a tissue sample chopping and spotting device. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment in vitro to allow cells to grow, reproduce and maintain their main structure and function; cell culture is also called cell cloning technology. Whether in biomedical research and development or in the production of cell products, cell culture is an indispensable step. Cell culture technology can transform a cell into a simple single-cell clone or directed differentiation into the target cell through large-scale culture, thereby obtaining the target cell line. It can also be used to study cell signal transduction, cell anabolism, cell growth and proliferation, etc.

[0003] In tissue cell culture, there are two main ways to obtain primitive cells: one is to digest the tissue block with digestive enzymes, and then obtain a single cell suspension through centrifugation, sieving, etc., and then culture it; the other is to directly chop the tissue block, spot it on the surface of the culture container, and culture it; among them, the tissue block spot culture method does not require enzyme digestion, the cells are not damaged, the cultured cells are highly active, and can be passaged many times.

[0004] The tissue block spot culture method generally involves cutting the animal tissue sample into pieces in a sterile container and then spreading the tissue sample on the bottom of the culture bottle with a cell scraper. This method has the following problems: first, the operation is an open operation, which increases the risk of microbial contamination; second, the tissue fragments are of different sizes when spread on the bottle, and the cell scraper can easily scrape the bottom of the culture bottle and damage the bottom of the bottle; third, it is time-consuming and further increases the chance of contamination. Utility Model Content

[0005] In order to solve the problems of inconvenient shearing and easy contamination of tissue samples, the utility model provides a tissue sample chopping and spotting device, and the technical solution adopted is as follows:

[0006] A tissue sample chopping and spotting device, characterized in that it comprises:

[0007] A tissue container, comprising a container body and a first hollow piston tube, wherein one end of the container body is open, one end of the piston of the first hollow piston tube is located inside the container body, and the other end is located outside the container body, and the first hollow piston tube is slidably connected to the container body along the axial direction;

[0008] An electric shear, detachably connected to the tissue container, for chopping up the tissue sample inside the container body;

[0009] The spotting device consists of a quantitative tube and a spotting tube connected to the bottom of the quantitative tube. An injection hole is arranged on one side of the upper part of the quantitative tube. A connector for detachably fixing the tissue container is fixedly connected to the injection hole. A second hollow piston tube is axially slidably connected to the quantitative tube. One end of the piston of the second hollow piston rod is located inside the quantitative tube, and the other end is located outside the quantitative tube.

[0010] Furthermore, a breathable film is arranged at one end of the piston of the first hollow piston tube and the second hollow piston tube, and an air filter membrane is arranged at the other end.

[0011] Furthermore, an external thread is provided at one end of the opening of the container body, and an internal thread which cooperates with the external thread is provided on the electric shear and the connecting piece.

[0012] Furthermore, the connecting piece is composed of a threaded section and a connecting section, the threaded section is provided with an internal threaded hole, the connecting section is provided with an arc surface for fitting with the outer surface of the quantitative tube on a side away from the threaded section, and the connecting section is provided with a connecting hole, the connecting hole is connected to the internal threaded hole and is distributed corresponding to the position of the injection hole on the quantitative tube.

[0013] Furthermore, the outer surface of the quantitative tube is provided with scale markings.

[0014] Furthermore, the tissue container, quantitative tube and spotting tube are all made of transparent material.

[0015] The beneficial effects of the utility model are:

[0016] 1. The tissue container, electric shears and spotting device can be detachably connected, and the tissue mincing and spotting processes are all closed, effectively reducing the risk of contamination;

[0017] 2. The tissue container and quantitative tube are respectively provided with air filter membrane and breathable film at the upper and lower ends. The air filter membrane can prevent microorganisms from being sucked into the device. The breathable film can push the tissue fragments and allow air to pass through, thereby maintaining the balance of air pressure inside and outside the device.

[0018] 3. The overall device adopts a split mechanism to push tissue fragments in sections and realize quantitative spotting through quantitative tubes. It can complete tissue fragmentation and spotting operations in a short time, effectively ensuring cell activity and inoculation amount, thereby improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the utility model

[0020] Figure 2 Schematic diagram of the internal structure of the organization container

[0021] Figure 3 Side view of electric shears

[0022] Figure 4 Schematic diagram of the connection structure between the tissue container and the electric shears

[0023] Figure 5 Schematic diagram of the spotting device structure

[0024] Figure 6 Schematic diagram of the structure of the quantitative tube and the second hollow piston tube

[0025] Figure 7 Right view of the connector

[0026] Figure 8 for Figure 7 Middle AA section view

[0027] Fig. 9 Schematic diagram of the connection structure between the tissue container and the sample setting device

[0028] in:

[0029] 1-tissue container, 101-container body, 102-first hollow piston tube, 103-first air filter membrane, 104-first air permeable membrane, 105-external thread;

[0030] 2-electric shears, 201-electric switch, 202-blade;

[0031] 3-spotting device, 301-quantitative tube, 302-spotting tube, 303-connecting piece, 3031-threaded section, 3032-connecting section, 3033-inner threaded hole, 3034-arc surface, 3035-connecting hole, 304-second hollow piston tube, 305-second air filter membrane, 306-second breathable film, 307-injection hole, 308-scale mark. DETAILED DESCRIPTION

[0032] The exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0033] In the description of the utility model, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the viewing direction or position relationship, and are only for the convenience of describing the utility model, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model.

[0034] like Figure 1 The tissue sample chopping and spotting device shown comprises a tissue container 1, an electric shearing device 2, and a spotting device 3. The tissue container 1 and the electric shearing device 2 are detachably and sealedly connected, and the tissue container 1 and the spotting device 3 are also detachably and sealedly connected.

[0035] Specifically, the tissue container 1 is as follows Figure 1 and Figure 2 As shown, it consists of a container body 101 and a first hollow piston tube 102. The container body 101 is a cylindrical shell without a cover at the right end, and an external thread 105 is arranged on the outer surface of the right end. The right end of the first hollow piston tube 102 is a piston and is connected to the inner wall of the container body 101, and the left end extends out of the left side of the container body 101 by a certain length. The first hollow piston tube 102 as a whole can be slidably connected to the container body 101 along the left and right directions. The first hollow piston tube 102 is divided into a rod body and a piston part. The inside of the rod body part and the piston part are both hollow and internally connected. A through hole is arranged at the left end of the rod body located outside the container body 101, and the through hole is connected to the internal cavity of the rod body, and a first air filter membrane 103 is fixed at the through hole, and a first breathable film 104 is arranged at the right end of the piston.

[0036] Electric shears 2 Figure 1 and Figure 3 As shown, the shear body includes a shear body and a blade 202. A motor is provided on the shear body. The motor output shaft is connected to the blade 202 for driving the blade 202 to rotate. An electric switch 201 is provided on the shear body for manually turning on and off the power supply to the motor. The shear body is also provided with an internal thread for threading with the right end of the container body 101, such as Figure 4 As shown, it is connected by thread form, which is easy to disassemble.

[0037] Spotting device 3 Figure 5 and Figure 6 As shown, it includes a quantitative tube 301 and a spotting tube 302. A second hollow piston tube 304 is slidably connected to the quantitative tube 301, and a connecting piece 303 is fixedly connected to the outer side of the upper part. The quantitative tube 301 is a cylindrical shell structure without a cover on the upper end. The quantitative tube 301 is provided with a discharge port at the lower end and a sampling hole 307 at the upper part, and scale marks 308 are provided from top to bottom on the outer surface. The second hollow piston tube 304 has the same structure as the first hollow piston tube 102, a second air filter membrane 305 is provided on the upper part of the rod body, and a second air permeable film 306 is provided on the lower end of the piston. The remaining structures will not be described in detail.

[0038] The structure of the connector 3 is as follows Figure 5 , Figure 7 and Figure 8As shown, it includes a threaded section 3031 and a connecting section 3032 integrally formed from left to right, an internal threaded hole 3033 is provided inside the threaded section 3031 for threaded connection with the container body 101, an arc surface 3034 is provided in the middle of the right side wall of the connecting section 3032, and connecting holes 3035 are distributed through the connecting section 3032. The connecting member 3 is fitted with the outer surface of the quantitative tube 302 through the arc surface 3034, and the internal threaded hole 3033 is connected with the injection hole 307 through the connecting hole 3035, so that the container body 101 is connected with the inside of the quantitative tube 302. When the spotting device 3 is connected to the tissue container 1, as shown Fig. 9 As shown, the quantitative tube 301 is threadedly connected to the container body 101 through a connector 303 .

[0039] In addition, in this embodiment, the container body 101 and the first hollow piston tube 102 in the tissue container 1 and the quantitative tube 301, the spotting tube 302, the connecting piece 303 and the second hollow piston tube 304 in the spotting device 3 are all made of transparent glass material, which is convenient for observing the internal situation of the device.

[0040] When using the device to perform tissue chopping, first pull the first hollow piston tube 102 to the left in the biosafety cabinet, put the tissue to be processed into the container body 101, and then assemble the tissue container 1 with the electric shear 2. Figure 4 , stand up the assembled device, with the tissue container 1 on top and the electric shears 2 on the bottom, turn on the electric switch 201 to chop the tissue; if the tissue fragments splash around and away from the blade 202 during the chopping process, turn off the electric switch 201, use the first hollow piston tube 102 to push the fragments to the blade 202, then pull back the first hollow piston tube 102, turn on the electric switch 201 again to chop the tissue; after chopping is completed, turn the device upside down, with the electric shears 2 on top and the tissue container 1 on the bottom, so that the tissue fragments are thrown away from the blade 202, and then turn off the electric switch 201.

[0041] When spotting tissue blocks, remove the electric shears 2 and connect the tissue container 1 to the spotting device 3. Figure 7As shown, the second hollow piston tube 304 is pulled to the top, so that the second air-permeable film 306 at the bottom of the second hollow piston tube 304 is flush with the upper end of the injection hole 307, and then the device is horizontally turned 180 degrees, so that the tube body of the sample tube 302 is facing upward, and then the second hollow piston tube 304 is pushed to pass the tissue fragments through the connecting piece 303 and the injection hole 307 in sequence and enter the interior of the quantitative tube 301, until the tissue fragments overflow a little from the tube body of the sample tube 302; then the device is turned over, the sample tube 302 is inserted into the culture bottle, and then the second hollow piston tube 304 is pushed, and the scale 308 is used to indicate the opening. Start quantitative spotting. If the tissue blocks in the quantitative tube 301 are used up, the spotting tube 302 can be unplugged, and the second hollow piston tube 304 can be pulled to the top to make the second air permeable film 306 flush with the upper end of the injection hole 307. Then the device is flipped horizontally 180 degrees to make the discharge port of the quantitative tube 301 face upward, and then the second hollow piston tube 304 is pushed to let the tissue fragments pass through the connecting piece 303 and the injection hole 307 in turn into the interior of the quantitative tube 301 until the tissue fragments overflow a little from the discharge port of the quantitative tube 301. Then the spotting tube 302 is inserted, the device is flipped, and spotting starts again until the spotting operation is completed.

[0042] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements can be made without departing from the principle of the present invention, and these improvements should also be regarded as the protection scope of the present invention.

Claims

1. A tissue sample chopping and spotting device, It is characterized in that include: A tissue container, comprising a container body and a first hollow piston tube, wherein one end of the container body is open, one end of the piston of the first hollow piston tube is located inside the container body, and the other end is located outside the container body, and the first hollow piston tube is slidably connected to the container body along the axial direction; An electric shear, detachably connected to the tissue container, for chopping up the tissue sample inside the container body; The spotting device consists of a quantitative tube and a spotting tube connected to the bottom of the quantitative tube. An injection hole is arranged on one side of the upper part of the quantitative tube. A connector for detachably fixing the tissue container is fixedly connected to the injection hole. A second hollow piston tube is axially slidably connected to the quantitative tube. One end of the piston of the second hollow piston rod is located inside the quantitative tube, and the other end is located outside the quantitative tube.

2. The tissue sample chopping and spotting device according to claim 1, It is characterized in that One end of the piston of the first hollow piston tube and the second hollow piston tube is provided with a breathable film, and the other end is provided with an air filter membrane.

3. The tissue sample chopping and spotting device according to claim 1, It is characterized in that An external thread is arranged at one end of the opening of the container body, and an internal thread which is matched and connected with the external thread is arranged on the electric shear and the connecting piece.

4. The tissue sample chopping and spotting device according to claim 1, It is characterized in that The connecting piece consists of a threaded section and a connecting section, the threaded section is provided with an internal threaded hole, the connecting section is provided with an arc surface for fitting with the outer surface of the quantitative tube on a side away from the threaded section, and the connecting section is provided with a connecting hole, the connecting hole is connected to the internal threaded hole and is distributed corresponding to the position of the injection hole on the quantitative tube.

5. The tissue sample chopping and spotting device according to claim 1, It is characterized in that The outer surface of the quantitative tube is provided with scale marks.

6. The tissue sample chopping and spotting device according to claim 1, It is characterized in that The tissue container and the spotting device are both made of transparent materials.

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