Cell culture bottle

By designing buffer pads and spring mechanisms in cell culture flasks, and filtering air with connecting tubes and filter membranes, the problem of difficulty in moving and slipping off in the prior art is solved, ensuring the cleanliness of the cell growth environment .

CN222160194UActive Publication Date: 2024-12-13VIVA BIOTECH
View PDF 1 Cites 0 Cited by

Patent Information

Application Number
CN202420770224.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-04-15
Publication Date
2024-12-13
Estimated Expiration
2034-04-15

AI Technical Summary

Technical Problem

Existing cell culture bottles are not convenient to move multiple bottles at the same time when taken, and the surface is smooth and easy to slide off. The need to open the bottle cap for oxygen may lead to bacterial contamination.

Method used

A cell culture bottle consisting of a placement box and a mounting tank was designed to fix the culture bottle with a buffer pad and a spring mechanism to prevent slipping, and to achieve air filtration into the bottle through the connecting tube and filter membrane to avoid opening the bottle cap.

Benefits of technology

It realizes convenient movement and fixation of multiple culture bottles to prevent slipping and filter bacteria in the air through the filter membrane to ensure the cleanliness of the cell growth environment.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN222160194U_ABST
    Figure CN222160194U_ABST
Patent Text Reader

Abstract

The utility model belongs to the technical field of cell culture flasks, and particularly relates to a cell culture flask which comprises a placement box, a plurality of mounting grooves are formed in the placement box, buffer pads are fixedly mounted on the inner walls of the mounting grooves, culture flasks are arranged in the buffer pads, and mounting blocks are fixedly mounted at the bottoms of the culture flasks and located in the mounting grooves. A limiting groove is formed in one side of the mounting block, a sliding groove communicating with the limiting groove is formed in one side of the mounting groove, the limiting block is slidably mounted in the sliding groove, one side of the limiting block extends to the limiting groove, and a plurality of springs fixed to the inner wall of the sliding groove are fixedly mounted on the side, away from the mounting groove, of the limiting block. When the whole device is used, it is ensured that a plurality of culture bottles can be carried at a time, convenience is achieved, the situation that the culture bottles slide down when a worker wears gloves and takes the culture bottles can be avoided, meanwhile, it is ensured that external air can enter the culture bottles, and bottle caps on the culture bottles do not need to be opened.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture bottles, in particular to a cell culture bottle. Background Art

[0002] Some cells and tissues can grow in suspension, but a considerable number of mammalian cells need to adhere to a surface. Therefore, in addition to having good transparency, non-toxicity and sterility, the culture flasks, culture plates and culture dishes used to provide an in vitro cell culture environment also need to be surface modified to enable them to adhere, divide and grow.

[0003] For example, the Chinese patent with publication number CN215856162U discloses a cell culture bottle, which belongs to the technical field of cell culture bottles. The cell culture bottle includes a bottle body and a support assembly. The cells to be cultured are first placed inside the bottle body. During the cell incubation process, when the bottle body needs to be tilted, the bottle body can be placed inside the placement rack, and then the electric push rod can be started. The electric push rod can push the tooth plate to move. At the same time, the first gear is connected to the tooth plate by transmission. The placement rack is set on one side of the support rack, and one end of the shaft rod is fixedly connected to the placement rack. Therefore, when the electric push rod pushes the tooth plate to move, the tooth plate can drive the first gear to rotate. When the first gear rotates, it will drive the shaft rod to rotate on the support rack, which can drive the placement rack to be tilted and adjusted, so that the operator does not need to tilt it by hand to achieve the tilting effect, thereby reducing the workload of the operator and improving practicality.

[0004] The above patent has the following problems:

[0005] This patent has some disadvantages when used, such as: it is not convenient to take multiple culture bottles at the same time when taking the culture bottles, and the surface of the culture bottles is relatively smooth when the staff takes the culture bottles. Some staff members wear sterilized gloves, which are easy to slip when taking the culture bottles. In addition, some cells need a certain amount of oxygen to grow, and some culture bottles need to open the bottle caps to allow oxygen to enter the culture bottles, which will cause bacteria in the air to enter the culture bottles and affect the growth of cells. In view of this, we propose a cell culture bottle. Utility Model Content

[0006] The purpose of the utility model is to provide a cell culture bottle to solve the problems raised in the above background technology.

[0007] In view of this, the utility model provides a cell culture bottle, comprising:

[0008] A placement box, wherein a plurality of mounting grooves are provided in the placement box, a buffer pad is fixedly installed on the inner wall of the mounting groove, a culture bottle is arranged in the buffer pad, a mounting block is fixedly installed at the bottom of the culture bottle and located in the mounting groove, a limiting groove is provided on one side of the mounting block, a slide groove connected to the limiting groove is provided on one side of the mounting groove, a limiting block is slidably installed in the slide groove, and one side of the limiting block extends to the limiting groove, a plurality of springs fixed to the inner wall of the slide groove are fixedly installed on the side of the limiting block away from the mounting groove, and an L-shaped rod is fixedly installed on the side of the limiting block away from the mounting groove, and the upper end of the L-shaped rod passes through the slide groove and extends to the outside, a connecting pipe is fixedly installed on the culture bottle, a sealing ring is plugged and installed in the connecting pipe, a plurality of filter membranes are fixedly installed in the sealing ring, and an anti-slip ring is fixedly installed on the culture bottle and located above the mounting groove.

[0009] In the technical solution, when it is necessary to move multiple culture bottles, the culture bottles together with the mounting block are inserted into the mounting groove through the buffer pad, so that the bottom of the mounting block squeezes the limit block. Since one side of the limit block is an inclined structure, when squeezed, the limit block will move together with the L-shaped rod in the direction away from the mounting groove. At this time, the plurality of springs will shrink when squeezed until the limit block completely enters the slide groove, and then the mounting block will continue to move downward until the bottom of the mounting block contacts the bottom of the mounting groove. Then, under the action of the rebound of the plurality of springs, the plurality of springs will move against the limit block until one side of the limit block is inserted into the limit groove, so that the mounting block and the culture bottle can be fixed in the mounting groove. At this time, the side wall of the culture bottle will be in close contact with the inner wall of the buffer pad.

[0010] When taking out the culture bottle, the staff member manually moves the L-shaped rod to make the L-shaped rod slide in the slide groove and move in the direction away from the installation groove. Subsequently, the L-shaped rod will drive the limit block to move together until one side of the limit block is completely moved out of the limit groove and the limit block is completely entered into the slide groove. At this time, the installation block will be released and then the culture bottle can be pulled upward to take it out. With the effect of the anti-slip ring, the staff member can hold the culture bottle more conveniently, and can avoid the situation that the culture bottle is easy to slip when the staff member takes it while wearing gloves. In addition, with the effect of the connecting tube, the sealing ring and several filter membranes, it can ensure that the outside air can enter the culture bottle without opening the bottle cap on the culture bottle. In addition, with the effect of several filter membranes, the bacteria in the air can be filtered cleanly, so that the air entering the culture bottle does not have any bacteria, ensuring that it will not affect the growth of cells.

[0011] In the above technical solution, further, one side of the limit block is in an inclined structure.

[0012] In this technical solution, it is ensured that when the culture bottle is pressed, the bottom of the mounting block will squeeze the limiting block so that the limiting block can enter the slide groove.

[0013] In the above technical solution, further, the side wall of the culture bottle is in close contact with the inner wall of the buffer pad, and the filtration pore size of the filter membrane is 0.2μm-0.45μm.

[0014] In this technical solution, it is ensured that the cushion pad can cushion the culture bottle, thereby ensuring that the cells in the culture bottle will not be damaged; it is ensured that the bacteria in the air can be filtered to avoid affecting the growth of the cells in the culture bottle.

[0015] In the above technical solution, further, the connecting tube is communicated with the inner cavity of the culture bottle, and the culture bottle is plug-fitted with the buffer pad.

[0016] In this technical solution, it is ensured that the outside air can enter the inner cavity of the culture bottle through the through tube; and it is ensured that the culture bottle can be inserted into the buffer pad.

[0017] In the above technical solution, further, the L-shaped rod is slidably connected to the slide groove, and the cross-section of the buffer pad is a U-shaped structure.

[0018] In the technical solution, it is ensured that the L-shaped rod can slide in the slide groove; it is ensured that the buffer pad can buffer the culture bottle.

[0019] In the above technical solution, further, the installation block is plug-fitted into the installation slot, and the limiting block is plug-fitted into the limiting slot.

[0020] In this technical solution, it is ensured that the mounting block can be inserted into the mounting groove; and it is ensured that the limiting block can be inserted into the limiting groove.

[0021] The beneficial effects of the utility model are:

[0022] 1. For the cell culture bottle, when the culture bottle is moved, the culture bottle together with the mounting block is inserted into the mounting groove through the buffer pad, so that the bottom of the mounting block squeezes the limit block. Because one side of the limit block is in an inclined structure, when squeezed, the limit block will move together with the L-shaped rod in a direction away from the mounting groove. At this time, the plurality of springs will shrink when squeezed until the limit block completely enters the slide groove, and then the mounting block will continue to move downward until the bottom of the mounting block contacts the bottom of the mounting groove. Then, under the action of the rebound of the plurality of springs, the plurality of springs will move against the limit block until one side of the limit block is inserted into the limit groove, so that the mounting block and the culture bottle can be fixed in the mounting groove. At this time, the side wall of the culture bottle will be in close contact with the inner wall of the buffer pad. Through the above operation, the remaining culture bottles can be fixed in the remaining mounting grooves respectively, ensuring that multiple culture bottles can be carried at one time, which is more convenient.

[0023] 2. When taking out the cell culture bottle, the staff can hold the culture bottle more conveniently due to the anti-slip ring, which can prevent the staff from slipping when taking the culture bottle while wearing gloves. In addition, due to the connecting tube, the sealing ring and a plurality of filter membranes, it can ensure that the outside air can enter the culture bottle without opening the bottle cap on the culture bottle. In addition, due to the action of the plurality of filter membranes, the bacteria in the air can be filtered out, so that the air entering the culture bottle is free of bacteria, ensuring that the growth of cells will not be affected. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0025] Figure 2 This is a schematic diagram of the internal structure of the sealing ring in the utility model;

[0026] Figure 3 This is a schematic diagram of the regional structure of the cell bottle in the utility model;

[0027] Figure 4 This is one of the schematic diagrams of the internal structure of the placement box in the utility model;

[0028] Figure 5 For this utility model Figure 4 The enlarged structural diagram at A in the middle;

[0029] Figure 6 This is the second schematic diagram of the internal structure of the placement box in the utility model;

[0030] Figure 7 For this utility model Figure 6 Enlarged structural diagram at B in the middle.

[0031] The symbols in the figure are:

[0032] 1. Placement box; 2. Culture bottle; 3. Sealing ring; 4. Anti-slip ring; 5. Connecting tube; 6. L-shaped rod; 7. Filter membrane; 8. Mounting block; 9. Limiting groove; 10. Mounting groove; 11. Buffer pad; 12. Slide groove; 13. Limiting block; 14. Spring. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present application to clearly describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application belong to the scope of protection of this application.

[0034] In the description of the present application, it should be noted that the terms used herein are only for describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. For ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be regarded as part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0035] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application are used to distinguish similar objects, and are not used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable under appropriate circumstances, so that the embodiments of the present application can be implemented in an order other than those illustrated or described here, and the objects distinguished by "first", "second", etc. are generally of one type, and the number of objects is not limited. For example, the first object can be one or more. In addition, "and / or" in the specification and claims represents at least one of the connected objects, and the character " / " generally indicates that the objects associated with each other are in an "or" relationship.

[0036] It should be noted that, in the description of the present application, the orientation or positional relationship indicated by terms such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description. Unless otherwise stated, these orientation words do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present application; the orientation words "inside and outside" refer to the inside and outside relative to the contour of each component itself.

[0037] It should be noted that, in the present application, the terms "comprise", "include" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "comprises one..." does not exclude the presence of other identical elements in the process, method, article or device including the element. In addition, it should be noted that the scope of the method and device in the embodiment of the present application is not limited to performing functions in the order shown or discussed, and may also include performing functions in a substantially simultaneous manner or in reverse order according to the functions involved, for example, the described method may be performed in an order different from that described, and various steps may also be added, omitted, or combined. In addition, the features described with reference to certain examples may be combined in other examples.

[0038] Embodiment 1:

[0039] See also Figure 1 - Figure 7 As shown, this embodiment provides a cell culture bottle, comprising:

[0040] A placing box 1 is provided, wherein the placing box 1 is provided with a plurality of mounting grooves 10, a buffer pad 11 is fixedly installed on the inner wall of the mounting groove 10, a culture bottle 2 is arranged in the buffer pad 11, a mounting block 8 is fixedly installed at the bottom of the culture bottle 2 and located in the mounting groove 10, a limiting groove 9 is provided on one side of the mounting block 8, a slide groove 12 connected with the limiting groove 9 is provided on one side of the mounting groove 10, a limiting block 13 is slidably installed in the slide groove 12, and one side of the limiting block 13 extends to the limiting groove 9, a plurality of springs 14 fixed to the inner wall of the slide groove 12 are fixedly installed on the side of the limiting block 13 away from the mounting groove 10, and an L-shaped rod 6 is fixedly installed on the side of the limiting block 13 away from the mounting groove 10, and the upper end of the L-shaped rod 6 passes through the slide groove 12 and extends to the outside, a connecting pipe 5 is fixedly installed on the culture bottle 2, a sealing ring 3 is inserted and installed in the connecting pipe 5, a plurality of filter membranes 7 are fixedly installed in the sealing ring 3, and an anti-slip ring 4 is fixedly installed on the culture bottle 2 and located above the mounting groove 10.

[0041] Among them, when it is necessary to move multiple culture bottles 2, the culture bottles 2 together with the mounting block 8 are inserted into the mounting groove 10 through the buffer pad 11, so that the bottom of the mounting block 8 squeezes the limit block 13. Because one side of the limit block 13 is an inclined structure, when squeezed, the limit block 13 will move together with the L-shaped rod 6 in the direction away from the mounting groove 10. At this time, the plurality of springs 14 will be squeezed and will shrink until the limit block 13 completely enters the slide groove 12, and then the mounting block 8 will continue to move downward until the bottom of the mounting block 8 contacts the bottom of the mounting groove 10. Then, under the action of the rebound of the plurality of springs 14, the plurality of springs 14 will push the limit block 13 to move until one side of the limit block 13 is inserted into the limit groove 9, so that the mounting block 8 and the culture bottle 2 can be fixed in the mounting groove 10. At this time, the side wall of the culture bottle 2 will be in close contact with the inner wall of the buffer pad 11.

[0042] When taking out the culture bottle 2, the staff member manually moves the L-shaped rod 6 to make the L-shaped rod 6 slide in the slide groove 12 and move in the direction away from the installation groove 10. Subsequently, the L-shaped rod 6 will drive the limiting block 13 to move together until one side of the limiting block 13 is completely moved out of the limiting groove 9 and the limiting block 13 is completely entered into the slide groove 12. At this time, the installation block 8 will be released and then the culture bottle 2 can be pulled upward to be taken out. Under the action of the anti-slip ring 4, the staff member can hold the culture bottle 2 more conveniently, and can avoid the situation that the culture bottle 2 is easy to slip when the staff member takes it while wearing gloves. In addition, under the action of the connecting pipe 5, the sealing ring 3 and the plurality of filter membranes 7, it can ensure that the outside air can enter the culture bottle 2 without opening the bottle cap on the culture bottle 2. In addition, under the action of the plurality of filter membranes 7, the bacteria in the air can be filtered cleanly, so that the air entering the culture bottle 2 does not contain any bacteria, ensuring that the growth of cells will not be affected.

[0043] Embodiment 2:

[0044] This embodiment provides a cell culture bottle, which, in addition to the technical solutions of the above embodiments, also has the following technical features: one side of the limit block 13 is in an inclined structure.

[0045] It is ensured that when the culture bottle 2 is pressed, the bottom of the mounting block 8 will squeeze the limiting block 13 so that the limiting block 13 can enter the sliding groove 12 .

[0046] Embodiment 3:

[0047] This embodiment provides a cell culture bottle, which, in addition to the technical solutions of the above embodiments, also has the following technical features: the side wall of the culture bottle 2 is in close contact with the inner wall of the buffer pad 11, and the filtration pore size of the filter membrane 7 is 0.2μm-0.45μm.

[0048] The cushion pad 11 is ensured to cushion the culture bottle 2 , thereby ensuring that the cells in the culture bottle 2 are not damaged; and the bacteria in the air are ensured to be filtered to avoid affecting the growth of the cells in the culture bottle 2 .

[0049] Embodiment 4:

[0050] This embodiment provides a cell culture bottle, which, in addition to the technical solutions of the above embodiments, also has the following technical features: the connecting tube 5 is connected to the inner cavity of the culture bottle 2, and the culture bottle 2 is plugged into the buffer pad 11.

[0051] Here, it is ensured that the external air can enter the inner cavity of the culture bottle 2 through the through pipe 5 ; and it is ensured that the culture bottle 2 can be inserted into the buffer pad 11 .

[0052] Embodiment 5:

[0053] This embodiment provides a cell culture bottle, which, in addition to the technical solutions of the above embodiments, also has the following technical features: the L-shaped rod 6 is slidably connected to the slide groove 12, and the cross-section of the buffer pad 11 is a U-shaped structure.

[0054] The L-shaped rod 6 is ensured to be able to slide in the slide groove 12 ; and the buffer pad 11 is ensured to be able to buffer the culture bottle 2 .

[0055] Embodiment 6:

[0056] This embodiment provides a cell culture bottle, which, in addition to the technical solutions of the above embodiments, also has the following technical features: the mounting block 8 is plugged into the mounting groove 10, and the limiting block 13 is plugged into the limiting groove 9.

[0057] Among them, it is ensured that the mounting block 8 can be inserted into the mounting groove 10 ; and it is ensured that the limiting block 13 can be inserted into the limiting groove 9 .

[0058] Working principle: when the culture bottle 2 needs to be moved, the culture bottle 2 is inserted into the installation groove 10 together with the mounting block 8 through the buffer pad 11, so that the bottom of the mounting block 8 squeezes the limit block 13. Because one side of the limit block 13 is an inclined structure, when squeezed, the limit block 13 will move together with the L-shaped rod 6 in the direction away from the installation groove 10. At this time, the plurality of springs 14 will shrink when squeezed until the limit block 13 completely enters the slide groove 12, and then the mounting block 8 continues to move downward until the bottom of the mounting block 8 contacts the bottom of the mounting groove 10. Then, under the action of the rebound of the plurality of springs 14, the plurality of springs 14 will push the limit block 13 to move until one side of the limit block 13 is inserted into the limit groove 9, so that the mounting block 8 and the culture bottle 2 can be fixed in the installation groove 10. At this time, the side wall of the culture bottle 2 will be in close contact with the inner wall of the buffer pad 11.

[0059] When taking out the culture bottle 2, the staff member manually moves the L-shaped rod 6 to make the L-shaped rod 6 slide in the slide groove 12 and move in the direction away from the installation groove 10. Subsequently, the L-shaped rod 6 will drive the limiting block 13 to move together until one side of the limiting block 13 is completely moved out of the limiting groove 9 and the limiting block 13 is completely entered into the slide groove 12. At this time, the installation block 8 will be released and then the culture bottle 2 can be pulled upward to be taken out. Under the action of the anti-slip ring 4, the staff member can hold the culture bottle 2 more conveniently, and can avoid the situation that the culture bottle 2 is easy to slip when the staff member takes it while wearing gloves. In addition, under the action of the connecting pipe 5, the sealing ring 3 and the plurality of filter membranes 7, it can ensure that the outside air can enter the culture bottle 2 without opening the bottle cap on the culture bottle 2. In addition, under the action of the plurality of filter membranes 7, the bacteria in the air can be filtered cleanly, so that the air entering the culture bottle 2 does not contain any bacteria, ensuring that the growth of cells will not be affected.

[0060] The embodiments of the present application are described above in conjunction with the accompanying drawings. In the absence of conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present application is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can also make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

Claims

1. A cell culture flask, characterized in that: include: A placement box (1), wherein a plurality of installation grooves (10) are provided in the placement box (1), a buffer pad (11) is fixedly installed on the inner wall of the installation groove (10), a culture bottle (2) is provided in the buffer pad (11), a mounting block (8) is fixedly installed at the bottom of the culture bottle (2) and located in the installation groove (10), a limiting groove (9) is provided on one side of the installation block (8), a sliding groove (12) connected to the limiting groove (9) is provided on one side of the installation groove (10), a limiting block (13) is slidably installed in the sliding groove (12), and one side of the limiting block (13) extends to the limiting groove (9) A plurality of springs (14) fixed to the inner wall of the slide groove (12) are fixedly installed on the side of the limit block (13) away from the installation groove (10), and an L-shaped rod (6) is fixedly installed on the side of the limit block (13) away from the installation groove (10), and the upper end of the L-shaped rod (6) passes through the slide groove (12) and extends to the outside. A connecting pipe (5) is fixedly installed on the culture bottle (2), a sealing ring (3) is inserted and installed in the connecting pipe (5), and a plurality of filter membranes (7) are fixedly installed in the sealing ring (3). An anti-slip ring (4) is fixedly installed on the culture bottle (2) and located above the installation groove (10).

2. A cell culture flask according to claim 1, characterized in that: One side of the limiting block (13) is in an inclined structure.

3. A cell culture bottle according to claim 1, characterized in that: The side wall of the culture bottle (2) is in close contact with the inner wall of the buffer pad (11), and the filtration pore size of the filter membrane (7) is 0.2 μm-0.45 μm.

4. A cell culture flask according to claim 1, characterized in that: The connecting tube (5) is in communication with the inner cavity of the culture bottle (2), and the culture bottle (2) is plugged into and matched with the buffer pad (11).

5. A cell culture bottle according to claim 1, characterized in that: The L-shaped rod (6) is slidably connected to the slide groove (12), and the cross section of the buffer pad (11) is in a U-shaped structure.

6. A cell culture flask according to claim 1, characterized in that: The mounting block (8) is plug-fitted into the mounting groove (10), and the limiting block (13) is plug-fitted into the limiting groove (9).

Citation Information

Patent Citations

  • Cell culture bottle

    CN215856162U