Concentration device and system for liquid sample and detection kit

Abstract

The utility model belongs to the field of biological detection, and discloses a liquid sample concentration device, a liquid sample concentration system and a detection kit. The concentration device comprises water absorption particles and a main body, the main body sequentially comprises a first cavity, a second cavity and a third cavity which are communicated with one another from top to bottom, the distances between the cavity walls of the same horizontal plane of the second cavity are sequentially reduced from top to bottom, and the third cavity is used for concentrating concentrated liquid samples. According to the concentration device for the liquid sample in some examples, water-absorbing particles which do not absorb water cannot enter the third chamber, so that the concentrated sample directly enters the third chamber and is prevented from being in direct contact with the water-absorbing particles. After absorbing water in a liquid sample and expanding, the water-absorbing particles can gradually and automatically migrate to the first chamber and finally enter the first chamber due to the structural characteristic that the second chamber is wide in top and narrow in bottom, so that the sample can be effectively prevented from being excessively concentrated and even sucked dry.

Description

Technical Field

[0001] The utility model belongs to the field of biological detection, and specifically relates to a liquid sample concentration device, a system and a detection kit. Background Art

[0002] The analysis of samples (including saliva, urine, blood, cerebrospinal fluid, etc.) is one of the important means to detect human health or human diseases. In fact, the analysis of human body fluids is to detect the effective target detection objects (including proteins, DNA / RNA, cells, compounds, etc.) contained in the body fluids to detect the health of the human body or whether the human body is sick. However, since the effective target detection objects in body fluids are often very low in content and the concentration is too low, if the body fluids are directly tested, the effective target detection objects cannot be detected, resulting in inaccurate judgment results. Therefore, it is necessary to concentrate these biological fluid samples to increase the concentration of the biomarkers being tested, so that they can be detected as positive using conventional immunoassay methods, reduce false negatives, and improve detection sensitivity.

[0003] In the prior art, those skilled in the art often use ultrafiltration equipment to concentrate the test fluid and use the concentrated test fluid to conduct immunoassay research. However, the concentration of the test fluid using ultrafiltration equipment is time-consuming and increases the operation cost and complexity, which is not suitable for point-of-care diagnosis (POCT).

[0004] In addition, there is an operation method in the prior art that uses a water-absorbing material to directly remove the molecules in the solution to concentrate it. The water-absorbing material used must not react chemically with the solution, not adsorb biological macromolecules, and be easy to separate from the solution. And water-absorbing particles prepared from water-absorbing materials are often used. Its raw materials can be roughly divided into powder system, cellulose system and synthetic polymer system. In the production process, the chemical reactions involved are graft polymerization of hydrophobic polymers and hydrophilic monomers, carboxymethylation reaction of hydrophobic polymers and cross-linking of water-soluble polymers. The water-absorbing particles contain strong hydrophilic groups, are insoluble in water, and are synthetic polymer materials that have the function of absorbing about 500 times to about 1,000 times their own weight of water.

[0005] There are methods of concentrating biological fluid samples using water-absorbing particles in the prior art, but the current method usually requires external forces such as centrifugation or squeezing to collect the concentrated liquid, which not only increases the concentration cost but also increases the complexity of the operation, which is not conducive to application scenarios such as screening. In addition, the method of concentrating biological fluid samples using water-absorbing particles in the prior art has the problem of biological fluid samples being sucked dry and the concentration effect is poor, and the recovery rate is low.

[0006] For example, CN 116057378 A discloses a concentrating device, a method for concentrating a test fluid, a method for testing a test fluid, and a test kit. The concentrating device includes a first container and a second container containing water-absorbing particles. A portion of the first container is composed of a discharge portion, and the discharge portion is formed by a porous membrane with a pore size of 0.05 um or more and 10 um or less. The water-absorbing particles absorb the solution contained in the test fluid injected into the first container, and a test fluid concentrate as a concentrated solution of the test fluid is generated in the first container. The second container recovers the test fluid concentrate discharged from the discharge portion by centrifugation. This method has the problem that the concentration time is difficult to control, and there is a problem that the solution in the sample is absorbed by the water absorbent. The concentration effect of this concentration method is difficult to control, and the loss of effective samples is large, which is not conducive to subsequent detection. Summary of the invention

[0007] The purpose of the utility model is to overcome at least one disadvantage of the prior art and to provide a liquid sample concentration device, system and detection kit.

[0008] The technical solution adopted by the utility model is:

[0009] The first aspect of the utility model provides: a liquid sample concentration device, comprising a main body, wherein the main body comprises, from top to bottom, a first chamber, a second chamber, and a third chamber that are interconnected;

[0010] The first chamber can accommodate water-absorbing particles that have swelled after absorbing water;

[0011] The second chamber is at least adjacent to the first chamber, and is wide at the top and narrow at the bottom, that is, the spacing between the chamber walls on the same horizontal plane decreases from top to bottom, and the minimum spacing is no greater than the diameter of the water-absorbing particles before water absorption, and the maximum spacing is less than the diameter of the water-absorbing particles after complete water absorption;

[0012] The third chamber is used to concentrate the concentrated liquid sample.

[0013] In some examples of the concentrating device, the passage between the first chamber and the third chamber cannot pass through water-absorbing particles that have not absorbed water.

[0014] In some examples of the concentrating device, the passage between the second chamber and the third chamber cannot pass through water-absorbing particles that have not absorbed water.

[0015] In some examples of the concentrating device, a guiding slope is provided at the bottom of the second chamber, and the guiding slope is inclined toward and connected to the third chamber.

[0016] In some examples of the concentrating device, the first chamber has a width of 5 to 20 mm, a length of 10 to 80 mm, and a height of 20 to 60 mm.

[0017] In some examples of the concentrating device, the second chamber has a width of 1.5 to 8 mm, a top length of 20 to 80 mm, a bottom length of 2 to 8 mm, and a height of 5 to 20 mm.

[0018] In some examples of the concentrator device, the volume of the third chamber is 0.15 mL to 3 mL.

[0019] In some examples of the concentrating device, the first chamber has a width of 5-20 mm, a length of 10-80 mm, and a height of 20-60 mm, and the second chamber has a width of 1.5-8 mm, a top length of 20-80 mm, a bottom length of 2-8 mm, and a height of 5-20 mm.

[0020] In some examples of the concentrator, the first chamber has a width of 5 to 20 mm, a length of 10 to 80 mm, and a height of 20 to 60 mm, and the volume of the third chamber is 0.15 mL to 3 mL.

[0021] In some examples of the concentrator device, the first chamber has a width of 5 to 20 mm, a length of 10 to 80 mm, and a height of 20 to 60 mm; the second chamber has a width of 1.5 to 8 mm, an upper length of 20 to 80 mm, a lower length of 2 to 8 mm, and a height of 5 to 20 mm; and the volume of the third chamber is 0.15 mL to 3 mL.

[0022] In some examples of the concentrating device, a sampling channel is further included, wherein the sampling channel is connected to the third chamber.

[0023] In some examples of the concentrating device, a lifting float is further included, wherein the lifting float includes a lifting ring and a floating plate connected to each other, the lifting ring is inserted into the second chamber, and after the lifting ring is inserted into the second chamber, the floating plate is higher than the second chamber.

[0024] In some examples of the concentrating device, a sieve plate is further included, wherein the sieve plate is disposed in the first chamber and separates the first chamber into two parts, an upper part and a lower part.

[0025] In some examples of the concentrating device, a support column is provided under the sieve plate and / or a support column is provided in the first chamber.

[0026] In some examples of the concentrating device, a containing box or an upper cover adapted to the opening of the first chamber is further included.

[0027] In some examples of the concentrating device, a detachable bottom plate is provided below the containing box and / or a sampling hole is provided on the upper cover.

[0028] In some examples of the concentrating device, the cross-sectional area of ​​the third chamber decreases from top to bottom.

[0029] In some examples of concentrating devices, the concentrating devices are made of a transparent material.

[0030] In some examples of concentrating devices, a scale is provided.

[0031] In some examples of the concentrating device, the diameter of the water-absorbing particles when not absorbing water is between 200 μm and 1 cm, preferably between 1.5 and 3.0 mm.

[0032] In some examples of the concentrating device, the particle size of the water-absorbing particles after complete water absorption is preferably 4.0 to 8.0 mm.

[0033] In some examples of concentrating devices, water-absorbing particles are prepared from existing commercial water-absorbing materials. Preferably, the water-absorbing particles are made of Sephadex G-10, Sephadex G-25, Sephadex G-50 coarse particles, PEG20000, sodium polyacrylate, potassium polyacrylate, polyacrylamide copolymer, acrylonitrile / propionamide copolymer, polyvinyl alcohol, water-absorbing resin, polyacrylic acid polymer, spherical cellulose particles, hydrogel, cellulose-or starch-acrylonitrile graft copolymer, acrylonitrile / propionamide copolymer, ethylene maleic anhydride copolymer, cross-linked maleic anhydride copolymer, anhydrous calcium chloride, calcium oxide, cross-linked carboxymethyl cellulose, and a mixture of one or more of cross-linked polyethylene oxide. These materials can be granulated and used alone, or they can be granulated and used after being wrapped by other inert materials. The water-absorbing particles used in the present invention have a stronger water-absorbing capacity. Compared with ordinary water-absorbing materials, the water-absorbing particles can reach hundreds to thousands of times their own weight after absorbing water. At the same time, the water-absorbing particles have a low adsorption capacity for protein to be measured, which can better achieve effective concentration of samples. The material of the water-absorbing particles needs to be selected according to the characteristics of the sample to be concentrated. The water-absorbing particles used should not react chemically with the sample, not adsorb or adsorb little to the molecules to be tested or concentrated, be easy to separate from the solution, not affect the subsequent processing of the concentrated sample, expand after absorbing water, and be elastic.

[0034] In some examples of concentration devices, the liquid sample is selected from body fluid samples such as urine, blood, saliva, cerebrospinal fluid, pleural effusion, etc.; or processed samples such as protein extracts, tissue extracts, nucleic acid extracts, blood diluents, intermediates that need to be concentrated in research and production, etc.

[0035] The above technical features can be combined arbitrarily without conflict.

[0036] The second aspect of the utility model provides:

[0037] A liquid sample concentration system comprises water-absorbing particles and the concentration device described in the first aspect of the utility model.

[0038] In some examples of the concentration system, the diameter of the water-absorbing particles when not absorbing water is between 200 μm and 1 cm, preferably between 1.5 and 3.0 mm.

[0039] In some examples of the concentration system, the particle size of the water-absorbing particles after complete water absorption is 4.0 to 8.0 mm.

[0040] The third aspect of the present invention provides:

[0041] A liquid sample detection kit comprises a concentration system and a detection reagent, wherein the concentration system is as described in the second aspect of the utility model.

[0042] The beneficial effects of the utility model are:

[0043] In some embodiments of the liquid sample concentration device of the utility model, the water-absorbing particles that have not absorbed water enter the first chamber and the second chamber, or only enter the second chamber. The particle size of the water-absorbing particles is larger than the narrowest part of the second chamber and cannot enter the third chamber. In this way, the concentrated sample directly enters the third chamber to avoid direct contact with the water-absorbing particles. After the water-absorbing particles expand after absorbing water in the liquid sample, they will gradually and automatically migrate to the first chamber due to the structural characteristics of the second chamber that is wide at the top and narrow at the bottom, and finally enter the first chamber, which can effectively prevent the sample from being sucked dry and further ensure the volume of the concentrated sample.

[0044] In some examples of the liquid sample concentration device of the utility model, the sample can be effectively concentrated in the first chamber and the second chamber. The setting of the second chamber plays a role of further concentration, thereby improving the concentration effect. In addition, the second chamber has a structure that is wide at the top and narrow at the bottom and is connected to the third chamber, so that the water-absorbing particles in the second chamber can be further concentrated close to the third chamber. Through the siphon effect of the water-absorbing particles, the sample in the third chamber can be concentrated, thereby promoting the concentration effect of the entire device. Therefore, the device has good concentration effect and high recovery rate.

[0045] The liquid sample concentration device of some examples of the utility model can concentrate the sample by standing still after adding the sample and the water-absorbing particles, without the need for operations such as centrifugal squeezing. It is easy to use and has low operating costs. After the concentrated sample is obtained, it can be tested by conventional immunoassay methods to achieve the purpose of reducing false negatives and improving detection sensitivity. It is suitable for POCT, screening and other application scenarios. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1Schematic diagram of the overall structure of some example concentration devices of the utility model, wherein W represents the width direction, H represents the height direction, and L represents the length direction.

[0047] Figure 2 It is a schematic diagram of the cross-sectional structure of some example concentration devices of the utility model.

[0048] Figure 3 It is another cross-sectional structural schematic diagram of some example concentration devices of the utility model.

[0049] Figure 4 It is a schematic diagram of the structure of the lifting floating plate of some examples of the concentration device of the utility model.

[0050] Figure 5 It is a schematic diagram of the structure of some example concentration device containing boxes of the utility model.

[0051] Figure 6 It is a schematic diagram of the structure of the upper cover of some examples of the concentration device of the utility model.

[0052] Figure 7 It is a schematic diagram of the structure of the sieve plates of some examples of the concentration device of the utility model.

[0053] Figure 8 It is a schematic cross-sectional structure diagram of another example of a concentrating device of the utility model.

[0054] Fig. 9 It is a schematic cross-sectional structure diagram of another example of a concentrating device of the utility model.

[0055] Fig.10 It is a schematic cross-sectional structure diagram of another example of a concentrating device of the utility model.

[0056] Fig.11 It is a schematic cross-sectional structure diagram of another example of a concentrating device of the utility model.

[0057] Fig.12 It is a schematic cross-sectional structure diagram of another example of a concentrating device of the utility model.

[0058] Reference numerals:

[0059] First chamber-1, sieve plate-11, support column-12, storage box-7, bottom plate-71

[0060] Second chamber-2, lifting floating plate-5, lifting ring-51, floating plate-52

[0061] Chamber 3 - 3

[0062] Sampling channel-4, upper cover-8, sampling hole-81. DETAILED DESCRIPTION

[0063] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements or elements having the same or similar functions from beginning to end. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0064] In the description of the present invention, it should be understood that descriptions involving orientation, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0065] In the description of the present utility model, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood to exclude the number itself, and "above", "below", "within" etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0066] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more features.

[0067] In the description of the present utility model, it should be noted that, unless otherwise clearly stipulated and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection or a movable connection, a detachable connection or a non-detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, and it can be internal connection between two elements, indirect connection, or an interactive relationship between two elements.

[0068] The following disclosure provides many different embodiments or examples for implementing different solutions of the present invention.

[0069] Reference Figures 1 to 12 , a liquid sample concentration device, comprising external water-absorbing particles that expand upon absorption of water and a main body, wherein the main body comprises, from top to bottom, a first chamber 1, a second chamber 2 and a third chamber 3 that are interconnected;

[0070] The first chamber 1 can accommodate water-absorbing particles that have swelled after absorbing water;

[0071] The second chamber is at least adjacent to the first chamber, and is wide at the top and narrow at the bottom, that is, the spacing between the chamber walls on the same horizontal plane decreases from top to bottom, and the minimum spacing is no greater than the diameter of the water-absorbing particles before water absorption, and the maximum spacing is less than the diameter of the water-absorbing particles after complete water absorption;

[0072] The third chamber 3 is used to collect and concentrate the liquid sample.

[0073] In some examples of the concentrating device, the maximum distance between the chamber walls of the second chamber 2 is located at the connection between the first chamber 1 and the second chamber 2 .

[0074] For the convenience of description, the length, width and height of the concentrator are as follows: Figure 1 shown.

[0075] In some examples of the concentrating device, the cavity walls on both sides of the second chamber 2 are arranged symmetrically.

[0076] In some examples of the concentrating device, the passage between the first chamber 1 and the third chamber 3 cannot pass through water-absorbing particles that have not absorbed water.

[0077] In some examples of the concentrating device, the passage between the second chamber 2 and the third chamber 3 cannot pass through the water-absorbing particles that have not absorbed water.

[0078] In order to coordinate with the phenomenon that the water-absorbing particles slowly expand and gradually increase in volume after absorbing water, the width of the second chamber 2 is set to a gradually decreasing structure from top to bottom, so that the water-absorbing particles can slowly rise to the first chamber 1 during the process of absorbing water and expanding, and can avoid the water-absorbing particles being stuck in the second chamber 2 due to the constant width. In some examples of the concentrating device, the second chamber 2 has two symmetrically arranged inclined cavity walls, the spacing between the cavity walls on the same horizontal plane decreases from top to bottom, and the angle ∠a between the two inclined cavity walls is 10 to 90°, preferably 10 to 40°, and more preferably 10 to 20°. The experimental data of the utility model inventor show that when the angle is 10-20°, almost all the water-absorbing particles can pop out of the second chamber 2 after fully absorbing water, the concentration effect is best, the utilization rate of the water-absorbing particles is best, and it is also beneficial for the concentrated sample liquid to enter the third chamber 3; when it is 20-40°, the water-absorbing particles can be more fully utilized, about 90%-95% of the water-absorbing particles can be fully absorbed and then separated from the second chamber 2; when it is 40-90°, about 70%-90% of the water-absorbing particles can be fully absorbed and then separated from the second chamber 2; at a larger angle, after the water-absorbing particles absorb water and expand, more of them will remain in the second chamber 2, which is not conducive to the full concentration of the sample.

[0079] In some examples of the concentrating device, the passages between the first chamber 1, the second chamber 2 and the third chamber 3 cannot pass through water-absorbing particles that have not absorbed water, that is, the passage between the first chamber 1 and the third chamber 3 cannot pass through water-absorbing particles that have not absorbed water, the passage between the second chamber 2 and the third chamber 3 cannot pass through water-absorbing particles that have not absorbed water, and the passage between the first chamber 1 and the second chamber 2 can pass through water-absorbing particles. In this way, water-absorbing particles can be prevented from entering the third chamber 3, preventing water-absorbing particles from occupying the space of the third chamber 3, and ensuring that the third chamber 3 contains only concentrated sample liquid.

[0080] The sizes of the first chamber 1, the second chamber 2 and the third chamber 3 can be set according to the amount of the treated liquid. Exemplarily, the width of the first chamber 1 ranges from 5 mm to 20 mm, preferably from 8 mm to 16 mm, preferably 14 mm, and the length of the first chamber 1 ranges from 10 mm to 80 mm, preferably from 30 mm to 60 mm, preferably 41 mm. Specifically, the height of the first chamber 1 is 20 to 60 mm, preferably from 40 to 50 mm, preferably 45 mm.

[0081] Exemplarily, the upper length of the second chamber 2 is 20-80 mm, preferably 30-60 mm, preferably 41 mm; the lower length is 2-8 mm, preferably 5 mm. The height of the second chamber 2 can be set according to actual concentration requirements. Exemplarily, the height of the second chamber 2 is 5-20 mm, preferably 11-14 mm, preferably 12.5 mm.

[0082] In some examples of the concentrating device, the width of the second chamber 2 is smaller than the width of the first chamber 1 .

[0083] In some examples of the concentrating device, the upper length of the second chamber 2 is greater than the lower length, so as to promote the liquid sample to concentrate toward the bottom of the second chamber 2 .

[0084] In some examples of the concentrating device, the cross-sectional area of ​​the second chamber 2 gradually decreases from top to bottom.

[0085] In some examples of the concentrator, the bottom of the second chamber 2 is provided with a flow guiding slope 21, which is inclined toward and connected to the third chamber 3. In this way, the concentrated sample liquid can flow back to the third chamber 3 better and more effectively. When adding samples, it can also play a role in guiding the samples, so that the samples can flow to the second chamber 2 more quickly. The setting of the flow guiding slope 21 can avoid the problem of sample retention caused by the plane, thereby affecting the recovery rate. Therefore, the flow guiding slope 21 plays a role in further improving the sample recovery rate.

[0086] In some examples of the concentrator, the cross-sectional area of ​​the third chamber 3 decreases from top to bottom. This allows the sample to be more concentrated, facilitates full absorption of the concentrated sample, and reduces residue. Specifically, the third chamber 3 may be a trapezoidal structure with the upper length greater than the lower length, or an inverted triangle or inverted cone structure. The volume of the third chamber 3 may be set according to the volume of the sample after concentration, such as being set to a volume of 0.15 mL to 1 mL.

[0087] In some examples of the concentrator, a sampling channel 4 is further included, and the sampling channel 4 is connected to the third chamber 3. In particular, the sampling channel 4 is arranged beside the first chamber 1 and the second chamber 2, and directly above the third chamber 3. Exemplarily, the shape of the sampling channel 4 can be set to be cylindrical or conical, and the diameter of the sampling channel 4 is larger than the tip of a common pipette, in order to ensure that a conventional pipette can be inserted into the bottom of the sampling channel for sampling, without the need for a special tip, thereby reducing operating costs.

[0088] In some examples of the concentrating device, a lifting floating plate 5 is further included, wherein the lifting floating plate 5 includes a lifting ring 51 and a floating plate 52 connected to each other, wherein the lifting ring 51 is inserted into the second chamber 2, and after the lifting ring 51 is inserted into the second chamber 2, the floating plate 52 is higher than the second chamber 2. Further, the distance from the edge of the lifting ring 51 to the second chamber 2 is smaller than the particle size of the water-absorbing particles before absorbing water, and the distance from the edge of the lifting floating plate 5 to the inner wall of the first chamber 1 is larger than the particle size of the water-absorbing particles before absorbing water. Furthermore, a placement guide rail for the lifting ring 51 is provided on the inner walls of the first chamber 1 and the second chamber 2, and the placement guide rail can better guide the lifting floating plate 5 to be placed in the second chamber 2. When in use, the lifting float 5 is placed in the second chamber 2, and the particles that have not absorbed water can enter the first chamber 1 and the second chamber 2. As the water-absorbing particles absorb water and expand, the floating plate 52 is gradually driven to move upward. At this time, the lifting ring 51 can gradually pull the water-absorbing particles in the second chamber 2 out of the second chamber 2; or the lifting float 5 is manually pulled upward to gradually pull the water-absorbing particles out of the second chamber 2. Thus, a predetermined amount of concentrated sample can be retained in the third chamber 3.

[0089] The setting of the lifting float 5 can solve the problem that when the sample volume is large and more water-absorbing particles need to be added, the water-absorbing particles in the second chamber 2 cannot be ejected into the first chamber 1 after absorbing water and expanding due to the large amount of material above and the excessive weight, thereby better ensuring the amount of concentrated sample.

[0090] In some examples of the concentrating device, a sieve plate 11 is further included, and the sieve plate 11 is placed in the first chamber 1 and separates the first chamber 1 into two parts, an upper part and an lower part. The sieve plate 11 separates the first chamber 1 into two parts, an upper part for initial concentration of the sample liquid, and a lower part for continuous concentration of the sample liquid.

[0091] In some examples of the concentrating device, a support column 12 is provided below the sieve plate 11 and / or the first chamber 1 is provided with a support column 12. The support column 12 can better control the volumes of the upper and lower parts.

[0092] When in use, water-absorbing particles are added to the continuous concentration reaction chamber, and then the sieve plate 11 is placed on the support column 12, and the water-absorbing particles are added to the initial concentration reaction chamber, and the sample is added. The highest liquid level of the sample is higher than the water-absorbing particles. A large amount of water-absorbing particles can be added to the initial concentration reaction chamber to quickly concentrate the sample. Furthermore, when the sample liquid level is lower than the sieve plate 11, the cavity below the sieve plate can still continue to concentrate the sample, achieving a multi-stage concentration effect, further improving the sample concentration effect. Another implementation is to place the sieve plate 11 on the support column 12, add water-absorbing particles into the first chamber 1, add the sample, and the highest liquid level of the sample is higher than the water-absorbing particles. When the sample is concentrated to be lower than the sieve plate 11, take out the sieve plate 11 and the water-absorbing particles, and add new water-absorbing particles to the sample to continue concentrating. After a period of time, the water-absorbing particles absorb water slowly or do not absorb water, resulting in a slow concentration speed or a long concentration time, thereby affecting the concentration effect. The sieve plate 11 and the water-absorbing particles can be taken out, and new water-absorbing particles can be added to the sample to continue concentrating, thereby achieving the effect of graded concentration and increasing the concentration speed.

[0093] In some examples of the concentration device, a container box 7 is further included that is adapted to the opening of the first chamber 1. The container box 7 can be used to hold water-absorbing particles and seal the container, and its volume can be set according to the amount of water-absorbing particles to be added. When the container is sealed by the container, the sample concentration reaction is also prevented from being contaminated by external environmental substances, thereby affecting the experimental effect.

[0094] In some examples of the concentrating device, a removable bottom plate 71 is provided below the containing box 7. In this way, the water-absorbing particles can be conveniently dropped into the first chamber 1 by removable bottom plate 71.

[0095] In some examples of the concentrator, an upper cover 8 is further included which matches the opening of the first chamber 1. In this way, the sample concentrator can be closed, and the contamination of the concentration process by the environment during use can be avoided.

[0096] In some examples of the concentrating device, the upper cover 8 is provided with a sampling hole 81. In this way, sampling can be achieved without removing the upper cover 8.

[0097] In some examples of the concentrator, it is made of a transparent material, which is convenient for observing the amount of sample added and the amount of sample after concentration. Furthermore, the concentrator is provided with scale lines, which is convenient for accurately observing the amount of sample added and the amount of sample after concentration.

[0098] The size of the water-absorbing particles can be selected according to the size of the concentrator. In some examples of concentrators, the particle size of the water-absorbing particles before absorbing water is 1.5 to 3.0 mm, and the particle size of the water-absorbing particles after absorbing water is 4.0 mm to 8 mm. The width of the second chamber 2 is set to 1 to 8 mm, preferably 3.5 mm. The width of the second chamber 2 of 3.5 mm is suitable for water-absorbing particles with a particle size of 1.5 to 3.0 mm.

[0099] The present invention does not impose any restrictions on the material of the liquid sample concentration device, and preferably uses a plastic material. The liquid sample concentration device can be formed by integrated injection molding.

[0100] Example 1

[0101] Reference Figures 1 to 12 , a liquid sample concentration device, comprising external water-absorbing and swelling water-absorbing particles and a main body, wherein the main body comprises, from top to bottom, a first chamber 1, a second chamber 2 and a third chamber 3 which are interconnected; the first chamber 1, the second chamber 2 and the third chamber 3 are as follows Figure 2 As shown, bounded by a dotted line.

[0102] The first chamber 1 can accommodate water-absorbing particles that have swelled after absorbing water;

[0103] The spacing between the walls of the second chamber 2 on the same horizontal plane decreases from top to bottom, with the minimum spacing being no greater than the diameter of the water-absorbing particles that have not absorbed water, and the maximum spacing being less than the diameter of the water-absorbing particles that have completely absorbed water;

[0104] The third chamber 3 is used to collect and concentrate the liquid sample.

[0105] When in use, according to the amount of liquid sample, put in an appropriate amount of water-absorbing particles, the water-absorbing particles enter the second chamber 2 or enter the first chamber 1 and the second chamber 2 at the same time, inject the desired concentrated sample solution, the water-absorbing particles absorb water to achieve sample concentration, as the water-absorbing particles absorb water, the volume expands to a certain volume, will be ejected from the second chamber 2 to the first chamber 1, the water-absorbing particles in the first chamber 1 continue to concentrate the sample, the water-absorbing particles in the second chamber 2 absorb water and expand and eject, and separate from the concentrated sample below to avoid over-concentration of the concentrated liquid. During use, after the sample is added, it only needs to be left to stand, and after the concentration is completed, the concentrated sample liquid is sucked for testing.

[0106] If necessary, the liquid sample to be concentrated and / or the water-absorbing particles may be added multiple times to obtain a sufficient amount of concentrated liquid or to achieve the concentration of a larger amount of sample.

[0107] Example 2

[0108] Reference Figures 1 to 12, a liquid sample concentration device, comprising external water-absorbing particles that expand upon absorption of water and a main body, wherein the main body comprises, from top to bottom, a first chamber 1, a second chamber 2 and a third chamber 3 that are interconnected;

[0109] The first chamber 1 can accommodate water-absorbing particles that have swelled after absorbing water;

[0110] The spacing of the walls of the second chamber 2 on the same horizontal plane decreases from top to bottom, the minimum spacing is no greater than the diameter of the water-absorbing particles that have not absorbed water, and the maximum spacing is less than the diameter of the water-absorbing particles that have completely absorbed water. A guide slope 21 is provided at the bottom of the second chamber 2, and the guide slope 21 is inclined to and connected to the third chamber 3;

[0111] The cross-sectional area of ​​the bottom of the third chamber 3 decreases from top to bottom and is tapered, and is used to concentrate the concentrated liquid sample;

[0112] The sampling channel 4 is arranged beside the first chamber 1 and the second chamber 2 and directly above the third chamber 3. The sampling channel 4 is connected with the first chamber 1, the second chamber 2 and the third chamber 3 through a slit.

[0113] Example 3

[0114] Reference Figures 1 to 12 , a liquid sample concentration device, comprising external water-absorbing particles that expand upon absorption of water and a main body, wherein the main body comprises, from top to bottom, a first chamber 1, a second chamber 2 and a third chamber 3 that are interconnected;

[0115] The first chamber 1 can accommodate water-absorbing particles that have swelled after absorbing water;

[0116] The spacing of the walls of the second chamber 2 on the same horizontal plane decreases from top to bottom, the minimum spacing is no greater than the diameter of the water-absorbing particles that have not absorbed water, and the maximum spacing is less than the diameter of the water-absorbing particles that have completely absorbed water. A guide slope 21 is provided at the bottom of the second chamber 2, and the guide slope 21 is inclined to and connected to the third chamber 3;

[0117] The cross-sectional area of ​​the bottom of the third chamber 3 decreases from top to bottom and is tapered, and is used to concentrate the concentrated liquid sample;

[0118] The lifting floating plate 5 comprises a lifting ring 51 and a floating plate 52 connected to each other, wherein the lifting ring 51 is inserted into the second chamber 2, and after the lifting ring 51 is inserted into the second chamber 2, the floating plate 52 is higher than the second chamber 2;

[0119] A sampling channel 4 is arranged beside the first chamber 1 and the second chamber 2 and directly above the third chamber 3. The sampling channel 4 is connected with the first chamber 1, the second chamber 2 and the third chamber 3 through a slit;

[0120] The upper cover 8 is matched with the opening of the first chamber 1 and is provided with a sampling hole 81 connected with the sampling channel 4 .

[0121] Example 4

[0122] Reference Figures 1 to 12 , a liquid sample concentration device, comprising external water-absorbing particles that expand upon absorption of water and a main body, wherein the main body comprises, from top to bottom, a first chamber 1, a second chamber 2 and a third chamber 3 that are interconnected;

[0123] The first chamber 1 can accommodate water-absorbing particles that have swelled after absorbing water, and a sieve plate 11 is provided therein, and the sieve plate 11 separates the first chamber 1 into two parts, an upper part and an lower part;

[0124] The spacing of the walls of the second chamber 2 on the same horizontal plane decreases from top to bottom, the minimum spacing is no greater than the diameter of the water-absorbing particles that have not absorbed water, and the maximum spacing is less than the diameter of the water-absorbing particles that have completely absorbed water. A guide slope 21 is provided at the bottom of the second chamber 2, and the guide slope 21 is inclined to and connected to the third chamber 3;

[0125] The cross-sectional area of ​​the bottom of the third chamber 3 decreases from top to bottom and is tapered, and is used to concentrate the concentrated liquid sample;

[0126] The sampling channel 4 is arranged beside the first chamber 1, the second chamber 2 and the third chamber 3, and the sampling channel 4 is connected with the first chamber 1, the second chamber 2 and the third chamber 3; an exemplary sampling channel is as follows Figure 2 As shown;

[0127] The receiving box 7 is adapted to the opening of the first chamber 1 and has a detachable bottom plate 71 disposed therebelow.

[0128] Exemplarily, the outer surface of the present concentration device is provided with three scale lines from top to bottom, namely, the first scale line, the second scale line and the third scale line. The first scale line and the second scale line are used to determine the volume range of the sample to be concentrated, and the third scale line is used to confirm the minimum volume after concentration. The setting of the scale lines improves the convenience of the sample concentration operation. There is no need to use a specific measuring tool to add liquid samples. The addition of samples is completed by adding to the first or second scale line, which can also prevent the sample from being over-concentrated.

[0129] Experimental example:

[0130] 1. Dilute LAM (lipoarabinomannan, LAM) antigen standard with negative urine to 0, 1, 2, 5, 10, 20, 50, 100, 200, 500, 1000 pg / ml to obtain negative urine treatment solution;

[0131] 2. Take 10 ml of the above negative urine treatment solution and add it to the sample concentrator of the embodiment of the utility model, wherein the sample concentrator has been pre-installed with water-absorbing particles; let it stand for 60 minutes to concentrate; use a pipette to draw 100 ul of the concentrated sample;

[0132] 3. Add the concentrated sample to the sample well of the LAM fluorescence chromatography detection card, react for 20 minutes, and then read the concentration value.

[0133] Comparative Example:

[0134] 1. Dilute the LAM antigen standard with negative urine to 0, 1, 2, 5, 10, 20, 50, 100, 200, 500, 1000 pg / ml to obtain negative urine treatment solution;

[0135] 2. Take 100ul of the above negative urine treatment solution and add it to the sample well of the LAM fluorescence chromatography detection card. After reacting for 20 minutes, read the concentration value.

[0136] The experimental results are shown in Table 1.

[0137] Table 1

[0138]

[0139] From Table 1, we can see that:

[0140] 1. The LAM antigen sample was concentrated by using the sample concentrating device of the utility model. The concentration of LAM antigen detected in the concentrated sample was about 5 times of the prepared concentration, indicating that the LAM antigen was effectively concentrated;

[0141] 2. When the LAM antigen concentration is less than 5pg / ml, the result of LAM fluorescence chromatography detection in the comparative example is 0, that is, LAM antigen less than 5pg / ml cannot be detected; in the experimental example, 1pg / ml LAM antigen is concentrated by the sample concentrator of the embodiment of the utility model, and the result of LAM fluorescence chromatography detection is 5.4, that is, it is concentrated 5.4 times. Therefore, biological fluid samples with low concentration of the biomarker to be tested can be detected, false negatives can be reduced, and detection sensitivity can be improved.

[0142] A method for concentrating a liquid sample comprises the following steps:

[0143] S01 injecting the sample to be concentrated into the concentration reaction chamber to which the water-absorbing particles are added, and letting it stand to allow the water-absorbing particles to absorb water molecules;

[0144] S02 After standing for a preset time H, or after the water-absorbing particles in the second chamber 2 absorb water and expand and eject into the first chamber, a concentrated sample is obtained.

[0145] Furthermore, the preset time H can be 60 minutes or set according to needs.

[0146] Exemplary, specific BSA sample concentration methods include:

[0147] S1) Add a number of water-absorbing particles to the concentration reaction chamber of the sample concentration device. The amount of water-absorbing particles and BSA sample added are shown in Table 1.

[0148] S2) Add BSA sample and mix by pipetting to disperse the water-absorbing particles;

[0149] S3) standing and concentrating;

[0150] S4) after the water-absorbing particles absorb water and swell and move upward into the first chamber, a concentrated sample is obtained;

[0151] S5) Take out the concentrated sample by pipette and measure the BSA concentration after concentration.

[0152] The experimental results are shown in Tables 2 to 4.

[0153] Table 2

[0154]

[0155] Table 3

[0156]

[0157] Table 4

[0158]

[0159] From the data in Tables 2 to 4, it can be seen that with this sample concentration method, when the sample concentration factor is more than 10 times, the recovery rate is still more than 75%, and the recovery rate is high. The concentrated sample can improve the detection sensitivity and reduce the false negative phenomenon. In addition, the 10-fold concentration effect can be achieved in 12 minutes, and the concentration efficiency is high.

[0160] The recovery rate is still 70% after concentration more than 10 times, which is suitable for concentration of large sample amounts.

[0161] From the data in Tables 2 to 4, it can be seen that the mass ratio of the water-absorbing particles to the sample volume ratio is in the range of 3 to 6 g: 20 ml, which can effectively concentrate the sample.

[0162] The above is a further detailed description of the utility model, which should not be regarded as a limitation on the specific implementation of the utility model. For ordinary technicians in the technical field to which the utility model belongs, simple deduction or replacement without departing from the concept of the utility model is within the protection scope of the utility model.

[0163] The above is a further detailed description of the utility model, which should not be regarded as a limitation on the specific implementation of the utility model. For ordinary technicians in the technical field to which the utility model belongs, simple deduction or replacement without departing from the concept of the utility model is within the protection scope of the utility model.

Claims

1. A liquid sample concentration device, comprising a main body, wherein the main body comprises, from top to bottom, a first chamber, a second chamber and a third chamber which are interconnected, characterized in that ； The first chamber can accommodate water-absorbing particles that have swelled after absorbing water; The second chamber is at least adjacent to the first chamber and is wide at the top and narrow at the bottom, that is, the spacing between the chamber walls on the same horizontal plane decreases from top to bottom, the minimum spacing is no greater than the diameter of a single water-absorbing particle that has not absorbed water, and the maximum spacing is greater than the diameter of a single water-absorbing particle that has not absorbed water; The third chamber is used to concentrate the concentrated liquid sample.

2. The concentrating device according to claim 1, characterized in that: The passages between the first chamber and the third chamber, and between the second chamber and the third chamber cannot pass through water-absorbing particles that have not absorbed water.

3. The concentrating device according to claim 1, characterized in that: A flow guiding slope is disposed at the bottom of the second chamber, and the flow guiding slope is inclined toward and connected to the third chamber.

4. The concentrating device according to claim 1, characterized in that: It also includes a sampling channel, which is communicated with the third chamber.

5. The concentrating device according to claim 1, characterized in that: It also includes a lifting floating plate, which includes a lifting ring and a floating plate connected to each other. The lifting ring is inserted into the second chamber. After the lifting ring is inserted into the second chamber, the floating plate is higher than the second chamber.

6. The concentrating device according to claim 1, characterized in that: It also includes a sieve plate, which is placed in the first chamber and separates the first chamber into two parts, an upper part and an lower part; and / or it also includes a containing box or an upper cover adapted to the opening of the first chamber.

7. The concentrating device according to claim 6, characterized in that: A support column is provided below the sieve plate and / or a support column is provided in the first chamber.

8. The concentrating device according to claim 6, characterized in that: A detachable bottom plate is provided below the containing box and / or a sampling hole is provided on the upper cover.

9. A liquid sample concentration system, characterized in that: The invention comprises water-absorbing particles and the concentrating device according to any one of claims 1 to 8.

10. A liquid sample detection kit, comprising a concentration system and a detection reagent, characterized in that: The concentrating system as claimed in claim 9.

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