Ochratoxin A fluorescent quantitative detection device

Abstract

The utility model discloses an ochratoxin A fluorescent quantitative detection device, which comprises a test strip, the test strip comprises a bottom plate, a sample pad, a combination pad, a reaction film and a water absorption pad, the sample pad, the combination pad, the reaction film and the water absorption pad are tightly connected and attached to the upper portion of the bottom plate, and a fluorescent microsphere marked ochratoxin A antibody is adsorbed on the combination pad. A detection line and a quality control line are arranged on the reaction film in the fluid diffusion direction, an ochratoxin A antigen is fixed on the detection line, and a goat anti-mouse IgG antibody is fixed on the quality control line; the top surface of the kit is provided with a groove for accommodating the reagent strip, the bottom surface of the kit is provided with a mounting groove, the mounting groove is positioned under the first groove body, a heating element is arranged in the mounting groove, and the bottom of the mounting groove is also provided with a bottom plate. The kit does not need to be additionally provided with an incubator, the handheld space of the reagent strip is expanded by arranging the kit, so that the operation is convenient, and the safety and the convenience of rapid detection are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of ochratoxin A detection, and more specifically, to an ochratoxin A fluorescence quantitative detection device. Background Art

[0002] Ochratoxin can contaminate corn, barley, wheat and other grains, as well as ham, fish products, feed and other food raw materials and their products. Among them, ochratoxin A (OTA) has the highest content and the greatest toxicity. It has strong kidney toxicity. When people continue to consume poisonous food or livestock and poultry continue to consume poisonous feed, acute symptoms may occur, and it may lead to serious chronic poisoning, carcinogenicity, teratogenicity, etc. The limit standard for ochratoxin A in feed and corn in my country does not exceed 100μg / kg. OTA fluorescent quantitative test strips use fluorescent immunocompetitive method to quickly detect the content of ochratoxin A in corn and feed. For example, the invention patent with authorization announcement number CN108776224B discloses an immunochromatographic test strip for detecting ochratoxin A. The problem is that the test strip needs to be incubated during the detection process. On the one hand, a separate incubator is required. On the other hand, due to the small size of the test strip, the test strip needs to be held in hand during incubation to avoid detection failure. Therefore, a convenient ochratoxin A fluorescent quantitative detection device is urgently needed. Summary of the invention

[0003] The utility model provides an ochratoxin A fluorescence quantitative detection device, which does not need to be additionally equipped with an incubator, and expands the hand-held space of a test strip by arranging a reagent kit to facilitate operation, thereby improving the safety and convenience of rapid detection.

[0004] In order to achieve these purposes and other advantages according to the utility model, a fluorescence quantitative detection device for ochratoxin A is provided, comprising:

[0005] A test strip comprises a bottom plate and a sample pad, a conjugation pad, a reaction membrane and a water-absorbing pad which are closely connected and attached to the bottom plate. The conjugation pad is adsorbed with fluorescent microspheres to label ochratoxin A antibodies. The reaction membrane is provided with a detection line and a quality control line along the fluid diffusion direction. The detection line is fixed with ochratoxin A antigen, and the quality control line is fixed with goat anti-mouse IgG antibodies.

[0006] The reagent box has a top surface with a groove to accommodate the test strip, and the groove includes a first groove body and a second groove body that are connected and communicated in sequence along the fluid diffusion direction, and the cross-sectional area and depth of the first groove body are both larger than those of the second groove body. The bottom surface of the reagent box is provided with a mounting groove, and the mounting groove is located directly below the first groove body. A heating element is provided in the mounting groove, and a bottom plate is also provided at the bottom of the mounting groove.

[0007] Preferably, the top surface of the reagent box is hinged with an upper cover, and the upper cover is provided with a sample addition port and an observation port at intervals. When the upper cover is rotated to cover the reagent box, the sample addition port and the observation port are located above the first trough body and the second trough body.

[0008] Preferably, a rotating handle is provided at the free end of the upper cover.

[0009] Preferably, a clamp is provided on the bottom surface of the upper cover, and a fastener is provided on the top surface of the reagent box.

[0010] Preferably, the heating element is a replaceable incubation bag, which is a non-woven bag encapsulated with iron powder, carbon powder, salt and vermiculite, and a release sticker is provided on one side surface of the non-woven bag.

[0011] Preferably, a heat conducting plate is provided between the first slot body and the mounting slot.

[0012] The utility model at least has the following beneficial effects:

[0013] First, the utility model improves the problem that an incubator is required during the detection process of the ochratoxin A fluorescent quantitative detection test strip. A heating element is provided to perform timed incubation of the test strip with the sample solution to be tested and the sample, thereby eliminating the need for an additional incubator. At the same time, the utility model improves the problem that the test strip is small in size and inconvenient to hold and operate. A test kit is designed to store the test strip, which is convenient for adding samples while holding the test kit, thereby improving the safety and convenience of the detection. The utility model is suitable for on-site rapid and accurate detection of various types of grains, grains, oils, and feeds.

[0014] Second, the test strips commonly used are conventional ochratoxin A fluorescent quantitative detection test strips. The groove of the test kit accommodates the test strips, and the groove is divided into a first groove body and a second groove body. The first groove body corresponds to the sample addition operation, and the second groove body corresponds to the observation operation. The depth and cross-sectional area of ​​the first groove body are larger than the second groove body. On the one hand, it is convenient for the operator to use a pipette to absorb the sample solution to be tested and then add the sample. The excess liquid enters the first groove body, which protects the operator's operating safety and reduces operating errors. A heating element is provided in the installation groove, which can be an electric heating element or a self-heating element. The heating temperature is controlled to be an incubation temperature, for example, 35~45°C, to improve the success rate of the operation. The bottom plate is set to enclose the heating element for heat conduction.

[0015] Third, when using the utility model, put the test strip into the groove, so that the sample pad is located above the first trough body, the absorbent pad is located above the second trough body, and the reagent box is placed horizontally. Use a pipette to draw 100 μL of the pre-treated sample solution to be tested and drop it onto the sample pad of the test strip to fully soak it with the solution. The excess sample solution to be tested is recovered to the first trough body. At the same time, the heating element is started, the heating temperature is controlled to be the incubation temperature, the incubation is timed, and the test strip is taken out after the timing is completed, and it is inserted into the fluorescence reader to read the result.

[0016] Fourth, the top surface of the reagent box is hinged with an upper cover, and the upper cover and the reagent box are rotatably connected by setting a hinge shaft at the tail end of the reagent box. The upper cover is provided with a sample addition port and an observation port, so that the upper cover covers the test strip to position and protect the test strip before operation. The setting of the rotating handle rotates the upper cover on the one hand to avoid contact with the sample solution to be tested by misoperation on the reagent box, and on the other hand, increases the distance between the sample addition position and the hand-held position, further improving the safety of operation. The upper cover and the reagent box are connected by snapping with a card and a fastener, so that the test strip can be fixed in position under slight touch and pressure. The heating element uses an incubation bag, and the iron powder is placed in a non-woven bag with air permeable holes. Before use, the encapsulated airtight outer packaging is removed to allow the iron powder to contact with oxygen in the air, control the amount of oxygen entering, and control the oxidation reaction rate, so as to achieve the time and temperature of heat release, and paste it in the groove through a release sticker. The first trough body and the mounting groove are designed as a heat conducting plate, such as a conductor, to accelerate heat conduction.

[0017] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a structural schematic diagram of a technical solution of the utility model in a buckled state;

[0019] Figure 2 This is a structural schematic diagram of another technical solution of the utility model in an open state. DETAILED DESCRIPTION

[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0021] It should be understood that terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0022] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial sources unless otherwise specified; in the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense, for example, they can be fixedly connected, set, or detachably connected, set, or connected and set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood in specific circumstances. The orientation or position relationship indicated by the terms "transverse", "longitudinal", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present utility model.

[0023] like Figure 1~2 As shown, the utility model provides a fluorescence quantitative detection device for ochratoxin A, comprising:

[0024] A test strip comprises a bottom plate and a sample pad, a conjugation pad, a reaction membrane and a water-absorbing pad which are closely connected and attached to the bottom plate. The conjugation pad is adsorbed with fluorescent microspheres to label ochratoxin A antibodies. The reaction membrane is provided with a detection line and a quality control line along the fluid diffusion direction. The detection line is fixed with ochratoxin A antigen, and the quality control line is fixed with goat anti-mouse IgG antibodies.

[0025] The reagent kit 1 has a groove on its top surface to accommodate the test strip, and the groove includes a first groove body 2 and a second groove body 3 that are connected and communicated in sequence along the fluid diffusion direction, and the cross-sectional area and depth of the first groove body 2 are both larger than those of the second groove body 3. The bottom surface of the reagent kit 1 is provided with a mounting groove 4, and the mounting groove 4 is located directly below the first groove body 2. A heating element is provided in the mounting groove 4, and a bottom plate is also provided at the bottom of the mounting groove 4.

[0026] In the above technical scheme, the problem that an incubator is required during the detection process of the ochratoxin A fluorescent quantitative detection test strip is improved. By setting a heating element, the test strip with the sample solution to be tested and the sample are incubated for a timed period, thereby eliminating the need for an additional incubator. At the same time, the problem that the test strip is small in size and inconvenient to hold and operate is improved. A test kit 1 is designed to store the test strip, which is convenient for adding samples while holding the test kit 1, thereby improving the safety and convenience of the detection, and is suitable for on-site rapid and accurate detection of various types of grain, oil, and feed.

[0027] The test strip commonly used is a conventional ochratoxin A fluorescent quantitative detection test strip. The groove of the test kit 1 accommodates the test strip, and the groove is divided into a first groove body 2 and a second groove body 3. The first groove body 2 corresponds to the sample addition operation, and the second groove body 3 corresponds to the observation operation. The depth and cross-sectional area of ​​the first groove body 2 are larger than the second groove body 3. On the one hand, it is convenient for the operator to use a pipette to absorb the sample solution to be tested and then add the sample. The excess liquid enters the first groove body 2, which protects the operator's operating safety and reduces operating errors. A heating element is provided in the mounting groove 4, which can be an electric heating element or a self-heating element. The heating temperature is controlled to be an incubation temperature, for example, 35~45°C, to improve the success rate of the operation. The bottom plate is set to enclose the heating element for heat conduction.

[0028] When using the utility model, the test strip is placed in the groove, the sample pad is located above the first trough body 2, the absorbent pad is located above the second trough body 3, the reagent kit 1 is placed horizontally, 100 μL of the pre-treated sample solution to be tested is sucked with a pipette and dripped onto the sample pad of the test strip to fully soak the solution, and the excess sample solution to be tested is recovered to the first trough body 2. At the same time, the heating element is started, the heating temperature is controlled to be the incubation temperature, the incubation is timed, and the test strip is taken out after the timing is completed, and inserted into the fluorescence reader to read the result.

[0029] In another technical solution, the top surface of the reagent box 1 is hinged with an upper cover 5, and the upper cover 5 is provided with a sample addition port 6 and an observation port 7 at intervals. When the upper cover 5 is rotated to cover the reagent box 1, the sample addition port 6 and the observation port 7 are located above the first trough body 2 and the second trough body 3. The top surface of the reagent box 1 is hinged with an upper cover 5, and the upper cover 5 is rotatably connected to the reagent box 1 by setting a hinge shaft at the rear end of the reagent box 1. The upper cover 5 is provided with a sample addition port 6 and an observation port 7, so that the upper cover 5 covers the test strip to position and protect the test strip before operation.

[0030] In another technical solution, the free end of the upper cover 5 is provided with a rotating handle 8. The setting of the rotating handle 8 can rotate the upper cover 5 to avoid contact with the sample solution to be tested on the reagent kit 1 due to misoperation, and can also increase the distance between the sample adding position and the hand-holding position, further improving the operation safety.

[0031] In another technical solution, a clamp is provided on the bottom surface of the upper cover 5, and a fastener is provided on the top surface of the reagent box 1. The upper cover 5 and the reagent box 1 are connected by the clamp and the fastener, so that the test strip can be fixed in position under slight pressure.

[0032] In another technical solution, the heating element is a replaceable incubation bag, which is a non-woven bag encapsulated with iron powder, carbon powder, salt, and vermiculite, and a release sticker is provided on one side of the non-woven bag. The heating element uses an incubation bag, and the iron powder is placed in a non-woven bag with air holes. Before use, the encapsulated airtight outer packaging is removed to allow the iron powder to contact with oxygen in the air, control the amount of oxygen entering, and control the oxidation reaction speed, so as to achieve the heat release time and temperature, and then stick it in the groove through the release sticker.

[0033] In another technical solution, a heat conducting plate is provided between the first slot body 2 and the mounting slot 4. The heat conducting plate, such as a conductor, is provided between the first slot body 2 and the mounting slot 4 to facilitate faster heat conduction.

[0034] The number of devices and processing scales described here are used to simplify the description of the utility model. Applications, modifications and variations of the utility model are obvious to those skilled in the art.

[0035] Although the implementation scheme of the utility model has been disclosed as above, it is not limited to the applications listed in the specification and implementation modes. It can be fully applied to various fields suitable for the utility model. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the utility model is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A fluorescence quantitative detection device for ochratoxin A, characterized in that: include: A test strip comprises a bottom plate and a sample pad, a conjugation pad, a reaction membrane and a water-absorbing pad which are closely connected and attached to the bottom plate. The conjugation pad is adsorbed with fluorescent microspheres to label ochratoxin A antibodies. The reaction membrane is provided with a detection line and a quality control line along the fluid diffusion direction. The detection line is fixed with ochratoxin A antigen, and the quality control line is fixed with goat anti-mouse IgG antibodies. The reagent box has a top surface with a groove to accommodate the test strip, and the groove includes a first groove body and a second groove body that are connected and communicated in sequence along the fluid diffusion direction, and the cross-sectional area and depth of the first groove body are both larger than those of the second groove body. The bottom surface of the reagent box is provided with a mounting groove, and the mounting groove is located directly below the first groove body. A heating element is provided in the mounting groove, and a bottom plate is also provided at the bottom of the mounting groove.

2. The ochratoxin A fluorescence quantitative detection device according to claim 1, characterized in that: The top surface of the reagent box is hinged with an upper cover, and the upper cover is provided with a sample adding port and an observation port at intervals. When the upper cover is rotated to cover the reagent box, the sample adding port and the observation port are located above the first slot body and the second slot body.

3. The ochratoxin A fluorescence quantitative detection device according to claim 2, characterized in that: The free end of the upper cover is provided with a rotating handle.

4. The ochratoxin A fluorescence quantitative detection device according to claim 2, characterized in that: The bottom surface of the upper cover is provided with a clamp, and the top surface of the reagent box is provided with a fastener.

5. The ochratoxin A fluorescence quantitative detection device according to claim 1, characterized in that: The heating element is a replaceable incubation bag, which is a non-woven bag encapsulated with iron powder, carbon powder, salt and vermiculite, and a release sticker is provided on one side surface of the non-woven bag.

6. The ochratoxin A fluorescence quantitative detection device according to claim 1 or 5, characterized in that: A heat conducting plate is disposed between the first slot body and the mounting slot.

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