Extraction and purification device for antibody

Abstract

The utility model provides an extraction and purification device for antibodies, which relates to the technical field of purification devices and comprises a refrigerating box, a top plate is fixedly mounted in the refrigerating box, a plurality of second electric telescopic rods which are uniformly distributed are fixedly mounted at the top of the top plate, and telescopic ends of the plurality of second electric telescopic rods penetrate through the top plate and are fixedly provided with fixing shafts. When the gravity column partition plate is replaced, a worker starts the electromagnet, the electromagnet is electrified to generate magnetic force, the iron clamping block enters the limiting groove due to attraction force, the gravity column partition plate can be taken down at the moment, then the clamping plate at the top of the gravity column partition plate of other specifications is inserted into the inserting groove, and when the clamping plate makes contact with the inclined face of the clamping block, the gravity column partition plate is replaced. The clamping blocks enter the limiting grooves under thrust, when the clamping grooves move to the positions beside the clamping blocks, the clamping blocks can enter the clamping grooves under the elastic force action of the springs, the clamping plates are fixed, therefore, gravity column partition plates of different specifications can be replaced, and the purification efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices, in particular to an extraction and purification device for antibodies. Background Art

[0002] Antibodies are special protein molecules that are used as in vitro diagnostic reagents, drugs for treating diseases, ligands for immunoaffinity chromatography, etc. They are widely used in life science research, biotechnology and medicine. In particular, antibodies, as core reagents for various immunoassays, play a vital role in the sensitivity and specificity of immunoassay results. The preparation of highly specific and high-titer antibodies is the basis of immunological technology.

[0003] In the prior art, serum or samples containing antibodies still contain some interfering proteins after centrifugation, so it is necessary to purify the antibodies in the samples. During experiments, gravity columns of different sizes are usually used for purification due to different sample volumes. Since the size of existing filter plates is fixed, it is difficult to improve the purification efficiency when purifying samples inside gravity columns of different sizes. Utility Model Content

[0004] The utility model aims to solve the problem in the prior art that the size of the existing filter plate is fixed, so it is difficult to quickly and completely separate the antibodies in the sample when purifying gravity columns of different sizes, and proposes an extraction and purification device for antibodies.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: an extraction and purification device for antibodies, comprising a cold storage box, a top plate fixedly installed inside the cold storage box, a plurality of evenly distributed second electric telescopic rods fixedly installed on the top of the top plate, the telescopic ends of the plurality of second electric telescopic rods all penetrate the top plate and are fixedly installed with fixed shafts, slots are provided at the bottoms of the plurality of fixed shafts, limiting grooves are symmetrically provided on both sides of the slots, electromagnets are fixedly installed on the inner walls of the two limiting grooves, a spring is fixedly installed on the side of the electromagnet close to the slot, a card block is fixedly installed on the side of the spring away from the electromagnet, a card plate is slidably installed inside the slot, a card slot is penetrated through the side wall of the card plate, and a gravity column partition is fixedly installed at the bottom of the card plate.

[0006] Preferably, a card seat is fixedly installed on the inner wall of the refrigerator, a plurality of evenly distributed through holes are opened through the top of the card seat, four evenly distributed storage slots are opened on the inner walls of the multiple through holes, third electric telescopic rods are fixedly installed on the inner walls of the four storage slots, support plates are fixedly installed on the telescopic ends of the four third electric telescopic rods, and a roller is provided at one end of the support plate.

[0007] Preferably, a fixing plate is fixedly mounted on the outer wall of the cold storage box, a plurality of storage boxes and a water pump are fixedly mounted on the top of the fixing plate, and a water inlet pipe is fixedly mounted between the input end of the water pump and the storage box.

[0008] Preferably, a plurality of evenly distributed first electric telescopic rods are fixedly mounted on one side of the refrigerator close to the fixed plate, and the telescopic ends of the plurality of first electric telescopic rods all penetrate the refrigerator and are fixedly mounted with a connecting plate.

[0009] Preferably, the output ends of the plurality of water pumps are fixedly mounted with water outlet pipes, one end of the plurality of water outlet pipes away from the water pumps passes through the refrigerator and is fixedly mounted with a telescopic hose, and one end of the telescopic hose passes through the connecting plate and is fixedly mounted with a nozzle.

[0010] Preferably, a door is hinged on one side of the refrigerator, and a glass is installed through the side wall of the door.

[0011] Preferably, a display screen is fixedly mounted on one side of the refrigerator.

[0012] Compared with the prior art, the advantages and positive effects of the utility model are:

[0013] 1. In the utility model, when replacing the gravity column partition, the staff starts the electromagnet, and the electromagnet is energized to generate magnetic force. The iron card block is attracted to enter the limit groove. At this time, the gravity column partition can be removed, and then the card plate on the top of the gravity column partition of other specifications is inserted into the slot. When the card plate contacts the inclined surface of the card block, the card block is pushed into the limit groove. When the card slot moves to the side of the card block, the card block can enter the card slot under the elastic force of the spring to fix the card plate. In this way, gravity column partitions of different specifications can be replaced, thereby improving the filtration efficiency.

[0014] 2. In the utility model, during purification, the staff places the reagent bottle of the antibody to be collected into the through hole, starts the two relative third electric telescopic rods, and the telescopic ends of the two third electric telescopic rods are stretched to push the two support plates and the two rollers to approach each other until the two rollers are in contact with the reagent bottle, and then starts the other two third electric telescopic rods to make the other two rollers approach each other. When the rollers are in contact with the reagent bottle, the rollers push the reagent bottle to move until the other two rollers are in contact with the reagent bottle. At this time, reagent bottles of different specifications can be clamped, and their centers are on the same horizontal line with the center of the through hole. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The utility model provides an overall stereogram of a device for extracting and purifying antibodies;

[0016] Figure 2A stereoscopic diagram from another perspective of a device for extracting and purifying antibodies proposed by the utility model;

[0017] Figure 3 A three-dimensional diagram of the internal structure of a refrigerator for an antibody extraction and purification device proposed in the utility model;

[0018] Figure 4 A fixed axis section view of a device for extracting and purifying antibodies is provided for the utility model;

[0019] Figure 5 The utility model provides a sectional view of a cartridge for an antibody extraction and purification device.

[0020] Legend: 1. Refrigerator; 2. Door; 3. Glass; 4. Display screen; 5. Fixed plate; 6. Storage box; 7. Water pump; 8. Water inlet pipe; 9. Water outlet pipe; 10. First electric telescopic rod; 11. Base; 12. Top plate; 13. Second electric telescopic rod; 14. Connecting plate; 15. Telescopic hose; 16. Nozzle; 17. Fixed shaft; 18. Gravity column partition; 19. Limiting groove; 20. Electromagnet; 21. Spring; 22. Block; 23. Card plate; 24. Card slot; 25. Slot; 26. Through hole; 27. Storage slot; 28. Third electric telescopic rod; 29. ​​Support plate; 30. Roller. DETAILED DESCRIPTION

[0021] In order to more clearly understand the above-mentioned purpose, features and advantages of the utility model, the utility model is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0022] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0023] Embodiment 1, as Figure 1-Figure 5As shown, the utility model provides an extraction and purification device for antibodies, including a refrigerator 1, a top plate 12 is fixedly installed inside the refrigerator 1, a plurality of evenly distributed second electric telescopic rods 13 are fixedly installed on the top of the top plate 12, the telescopic ends of the plurality of second electric telescopic rods 13 all penetrate the top plate 12 and are fixedly installed with a fixed shaft 17, slots 25 are provided at the bottom of the plurality of fixed shafts 17, limiting grooves 19 are symmetrically provided on both sides of the slots 25, electromagnets 20 are fixedly installed on the inner walls of the two limiting grooves 19, a spring 21 is fixedly installed on the side of the electromagnet 20 close to the slot 25, a clamping block 22 is fixedly installed on the side of the spring 21 away from the electromagnet 20, a clamping plate 23 is slidably installed inside the slot 25, a clamping groove 24 is provided through the side wall of the clamping plate 23, and a gravity column partition 18 is fixedly installed at the bottom of the clamping plate 23.

[0024] The effect achieved by the entire embodiment 1 is that when replacing the gravity column partition 18, the staff starts the electromagnet 20, and the electromagnet 20 is energized to generate magnetic force. The iron block 22 is attracted and enters the limit groove 19. At this time, the gravity column partition 18 can be removed, and then the card plate 23 on the top of the gravity column partition 18 of other specifications is inserted into the slot 25. When the card plate 23 contacts the inclined surface of the card block 22, the card block 22 is pushed into the limit groove 19. When the card slot 24 moves to the side of the card block 22, the card block 22 can enter the card slot 24 under the elastic force of the spring 21 to fix the card plate 23. In this way, gravity column partitions 18 of different specifications can be replaced, thereby improving the filtration efficiency.

[0025] Embodiment 2, as Figure 1-Figure 5 As shown, a card seat 11 is fixedly installed on the inner wall of the refrigerator 1, and a plurality of evenly distributed through holes 26 are opened through the top of the card seat 11. Four evenly distributed storage slots 27 are opened on the inner walls of the plurality of through holes 26. Third electric telescopic rods 28 are fixedly installed on the inner walls of the four storage slots 27. Support plates 29 are fixedly installed at the telescopic ends of the four third electric telescopic rods 28, and a roller 30 is provided at one end of the support plate 29.

[0026] Furthermore, a fixed plate 5 is fixedly installed on the outer wall of the cold storage box 1, a plurality of storage boxes 6 and a water pump 7 are fixedly installed on the top of the fixed plate 5, and a water inlet pipe 8 is fixedly installed between the input end of the water pump 7 and the storage box 6.

[0027] Furthermore, a plurality of evenly distributed first electric telescopic rods 10 are fixedly installed on one side of the refrigerator 1 close to the fixed plate 5 , and the telescopic ends of the plurality of first electric telescopic rods 10 all penetrate the refrigerator 1 and are fixedly installed with a connecting plate 14 .

[0028] Furthermore, the output ends of the multiple water pumps 7 are fixedly installed with water outlet pipes 9, and one end of the multiple water outlet pipes 9 away from the water pumps 7 passes through the refrigerator 1 and is fixedly installed with a telescopic hose 15, and one end of the telescopic hose 15 passes through the connecting plate 14 and is fixedly installed with a nozzle 16.

[0029] Furthermore, a door 2 is hinged on one side of the cold storage box 1, and a glass 3 is installed through the side wall of the door 2 to facilitate the staff to observe the situation in the reagent bottle.

[0030] Furthermore, a display screen 4 is fixedly mounted on one side of the refrigerator 1 for displaying the internal temperature of the refrigerator 1 .

[0031] The effect achieved by the entire embodiment 2 is that during purification, the staff places the reagent bottle of the antibody to be collected into the through hole 26, starts the two relative third electric telescopic rods 28, and the telescopic ends of the two third electric telescopic rods 28 are stretched to push the two support plates 29 and the two rollers 30 to approach each other until the two rollers 30 contact the reagent bottle, and then starts the other two third electric telescopic rods 28 to make the other two rollers 30 approach each other. When the rollers 30 contact the reagent bottle, the rollers 30 push the reagent bottle to move until the other two rollers 30 contact the reagent bottle. At this time, reagent bottles of different specifications can be clamped, and their centers are on the same horizontal line with the center of the through hole 26.

[0032] Working principle: During purification, the staff places the reagent bottle of the antibody to be collected into the through hole 26, and starts the two relative third electric telescopic rods 28. The telescopic ends of the two third electric telescopic rods 28 are stretched to push the two support plates 29 and the two rollers 30 to approach each other until the two rollers 30 contact the reagent bottle, and then start the other two third electric telescopic rods 28 to make the other two rollers 30 approach each other. When the rollers 30 contact the reagent bottle, the rollers 30 push the reagent bottle to move until the other two rollers 30 contact the reagent bottle. At this time, reagent bottles of different specifications can be clamped, and their centers are on the same horizontal line with the center of the through hole 26. Then the staff starts the second electric telescopic rod 13, and the telescopic end of the second electric telescopic rod 13 stretches to push the gravity column partition 18 down until the gravity column partition 18 contacts the bottom surface of the reagent bottle. Then the staff starts the first electric telescopic rod 10, and the telescopic end of the first electric telescopic rod 10 stretches to push the nozzle 16 to move above the reagent bottle, and starts the water pump 7. The water pump 7 puts the inorganic salt solution inside the storage box 6 into the water outlet pipe 9 and finally enters the reagent bottle from the nozzle 16 to start the reaction. After the reaction is over, the staff starts the second electric telescopic rod 13, and the telescopic end of the second electric telescopic rod 13 retracts, driving the gravity column partition 18 to rise. When the gravity column partition 18 rises, the solids in the solution will be filtered and removed, and then the third electric telescopic rod 28 is started, and the roller 30 retracts, and the reagent bottle can be taken out. When replacing the gravity column partition 18, the staff starts the electromagnet 20, and the electromagnet 20 is energized to generate magnetic force. The iron block 22 is attracted and enters the limit groove 19. At this time, the gravity column partition 18 can be removed, and then the card plate 23 on the top of the gravity column partition 18 of other specifications is inserted into the slot 25. When the card plate 23 contacts the inclined surface of the card block 22, the card block 22 is pushed into the limit groove 19. When the card slot 24 moves to the side of the card block 22, the card block 22 can enter the card slot 24 under the elastic force of the spring 21 to fix the card plate 23. In this way, gravity column partitions 18 of different specifications can be replaced, thereby improving the filtration efficiency.

[0033] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A device for extracting and purifying antibodies, comprising a refrigerator (1), characterized in that: A top plate (12) is fixedly installed inside the refrigerator (1), and a plurality of evenly distributed second electric telescopic rods (13) are fixedly installed on the top of the top plate (12). The telescopic ends of the plurality of second electric telescopic rods (13) all penetrate the top plate (12) and are fixedly installed with fixed shafts (17). The bottoms of the plurality of fixed shafts (17) are provided with slots (25). The slots (25) are symmetrically provided with limiting grooves (19) on both sides thereof. The inner walls of the two limiting grooves (19) are fixedly installed with electromagnets (20). A spring (21) is fixedly installed on the side of the electromagnet (20) close to the slot (25), and a clamping block (22) is fixedly installed on the side of the spring (21) away from the electromagnet (20). A clamping plate (23) is slidably installed inside the slot (25), and a clamping groove (24) is provided through the side wall of the clamping plate (23). A gravity column partition (18) is fixedly installed on the bottom of the clamping plate (23).

2. The device for extracting and purifying antibodies according to claim 1, characterized in that: A card seat (11) is fixedly mounted on the inner wall of the refrigerator (1); a plurality of evenly distributed through holes (26) are formed through the top of the card seat (11); four evenly distributed storage slots (27) are formed on the inner walls of the multiple through holes (26); third electric telescopic rods (28) are fixedly mounted on the inner walls of the four storage slots (27); support plates (29) are fixedly mounted on the telescopic ends of the four third electric telescopic rods (28); a roller (30) is provided at one end of the support plate (29).

3. The device for extracting and purifying antibodies according to claim 1, characterized in that: A fixing plate (5) is fixedly mounted on the outer wall of the cold storage box (1), a plurality of storage boxes (6) and a water pump (7) are fixedly mounted on the top of the fixing plate (5), and a water inlet pipe (8) is fixedly mounted between the input end of the water pump (7) and the storage box (6).

4. The device for extracting and purifying antibodies according to claim 1, characterized in that: A plurality of evenly distributed first electric telescopic rods (10) are fixedly mounted on one side of the refrigerator (1) close to the fixed plate (5); the telescopic ends of the plurality of first electric telescopic rods (10) all penetrate the refrigerator (1) and are fixedly mounted with a connecting plate (14).

5. The device for extracting and purifying antibodies according to claim 3, characterized in that: The output ends of the plurality of water pumps (7) are all fixedly mounted with water outlet pipes (9); one end of the plurality of water outlet pipes (9) away from the water pumps (7) passes through the refrigerator (1) and is fixedly mounted with a telescopic hose (15); one end of the telescopic hose (15) passes through the connecting plate (14) and is fixedly mounted with a nozzle (16).

6. The device for extracting and purifying antibodies according to claim 1, characterized in that: A door (2) is hingedly connected to one side of the refrigerator (1), and a glass (3) is installed through the side wall of the door (2).

7. The device for extracting and purifying antibodies according to claim 1, characterized in that: A display screen (4) is fixedly mounted on one side of the refrigerator (1).

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