Anaerobic jar

Abstract

The utility model discloses an anaerobic jar. The anaerobic jar comprises a shell, a cover, a culture dish placing rack and a test tube placing rack, an anaerobic culture cavity with an opening in the top end is defined by the shell, a culture dish placing frame which can be lifted upwards and is of a multi-layer structure is arranged in the anaerobic culture cavity, the culture dish placing frame is in a relatively closed state, an opening is formed in the face, facing the side wall of the shell, of the culture dish placing frame, and a relatively closed cavity for containing culture dishes is defined by the culture dish placing frame and the side wall of the anaerobic culture cavity; a test tube placing rack for accommodating test tubes and a plurality of independent grooves are arranged beside the culture dish placing rack in the anaerobic culture cavity; the cover is matched with an opening in the top end of the anaerobic culture cavity, and a vacuumizing interface is formed in the cover. The anaerobic jar is simple in structure, convenient to use, convenient to move and carry, capable of conducting anaerobic culture simply and efficiently at low cost, capable of being used as a teaching aid for anaerobic culture and capable of being used for low-cost anaerobic bacteria culture.

Description

Technical Field

[0001] The present application relates to the technical field of anaerobic culture, and in particular to an anaerobic tank. Background Art

[0002] In microbiological experiments, culturing under anaerobic conditions is essential for isolating and culturing anaerobic bacteria. At present, in microbiological experiments, anaerobic incubators, namely anaerobic workstations or anaerobic glove boxes, are usually used to isolate and culture anaerobic bacteria. Anaerobic incubators are special devices for culturing and operating bacteria in an oxygen-free environment, providing a systematic and scientific working area for anaerobic culture.

[0003] However, traditional anaerobic incubators or similar equipment are large in size, expensive, and require a fixed microbiological laboratory or a fixed space for placement, which is inconvenient to use. Especially when used as a teaching tool, the existing anaerobic incubator is extremely inconvenient to use. Therefore, it is urgent to develop an anaerobic tank that is low in cost and easy to move and carry. Utility Model Content

[0004] The purpose of this application is to provide a new anaerobic tank.

[0005] This application adopts the following technical solutions:

[0006] The present application discloses an anaerobic tank, comprising a shell, a lid, a culture dish rack and a test tube rack; the shell encloses an anaerobic culture cavity with an opening at the top, a culture dish rack with a multi-layer structure that can be lifted upward is arranged in the anaerobic culture cavity, the culture dish rack is in a relatively closed state, is opened on a side facing the side wall of the shell, and encloses a relatively closed cavity for accommodating culture dishes with the side wall of the anaerobic culture cavity; in the anaerobic culture cavity, a test tube rack for accommodating test tubes and a plurality of independent grooves are arranged next to the culture dish rack; the lid matches the top opening of the anaerobic culture cavity, and a vacuum extraction interface is opened on the lid.

[0007] Among them, the culture dish rack is in a relatively closed state, and the cavity accommodating the culture dish is in a relatively closed state, which means that the cavity accommodating the culture dish is separated from the test tube rack placed next to it, and is not completely sealed; it can be understood that when the anaerobic culture cavity is vacuumed, the cavity accommodating the culture dish will also be vacuumed; therefore, the culture dish rack and the cavity accommodating the culture dish can circulate gas with the anaerobic culture cavity.

[0008] It should be noted that the anaerobic tank of the present application is specially designed for small and medium-sized anaerobic culture and anaerobic culture teaching aids in microbiology laboratories. It is simple and convenient to use. For example, in one implementation of the present application, the anaerobic tank is only a cylindrical tube with a diameter of 9-20cm and a height of 15-30cm, which not only takes up little space, but also is easy to move and carry. The present application provides a simple, low-cost, and efficient anaerobic tank, which can be used not only for teaching aids, but also for low-cost cultivation of anaerobic bacteria, and is suitable for anaerobic bacterial conditional cultivation in microbiology experiments. It can be understood that the anaerobic tank of the present application can also be used for the cultivation of some microaerobic bacteria, that is, the cultivation of some micro-oxygen-absorbing strains.

[0009] Preferably, the top of the culture dish rack has a handle to facilitate lifting the culture dish rack.

[0010] Preferably, the culture dish placement rack is a drawer-type structure, which is pulled out from a side opening to facilitate the placement of culture dishes.

[0011] Preferably, the culture dish rack and the test tube rack are connected together.

[0012] Preferably, the test tube rack is provided with holes for accommodating test tubes of different sizes.

[0013] Preferably, the interior of the cover is designed with an air leakage-proof rubber ring.

[0014] Preferably, the number of the grooves is at least 4, for placing different reagents.

[0015] Preferably, the cover is also provided with a one-way exhaust valve.

[0016] Preferably, the anaerobic tank is in the form of a cylinder.

[0017] Preferably, the anaerobic tank is a cylindrical cylinder with a diameter of 9-20 cm and a height of 15-30 cm.

[0018] Preferably, a plurality of buckles are provided on the outer side wall of the cover, and correspondingly, a buckle buckle area matching with the buckles is provided on the outer side wall of the shell, so as to fix the cover on the shell.

[0019] Preferably, a handle is provided on the outer side wall or the cover of the anaerobic tank to facilitate picking up the anaerobic tank.

[0020] The beneficial effects of this application are:

[0021] The anaerobic jar of the present application has a simple structure, is easy to use, and is easy to move and carry. It can perform anaerobic culture simply, at low cost, and efficiently. It can not only be used as a teaching aid for anaerobic culture, but also can be used for low-cost anaerobic bacteria culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1It is a schematic diagram of the structure of the anaerobic tank in the embodiment of the present application;

[0023] Figure 2 It is a schematic diagram of the structure of the culture dish rack and the test tube rack inside the anaerobic tank in the embodiment of the present application. DETAILED DESCRIPTION

[0024] The present application is described in detail below by means of specific embodiments in combination with the accompanying drawings. The following embodiments are only used to illustrate the present application and should not be construed as limiting the present application.

[0025] Example

[0026] The anaerobic tank in this example is Figure 1 As shown, it includes a shell 1, a lid 2, a culture dish rack 3 and a test tube rack 4; the shell 1 encloses an anaerobic culture cavity 11 with an opening at the top, and a culture dish rack 3 with a multi-layer structure that can be lifted upward is arranged in the anaerobic culture cavity 11, and the culture dish rack 3 is in a relatively closed state, with an opening on the side facing the side wall of the shell 1, and encloses a relatively closed cavity for accommodating culture dishes with the side wall of the anaerobic culture cavity 11; in the anaerobic culture cavity 11, a test tube rack 4 for accommodating test tubes 5 is arranged next to the culture dish rack 3, and a plurality of independent grooves; the lid 2 matches the top opening of the anaerobic culture cavity 11, and a vacuum interface 21 and a one-way exhaust valve 22 are opened on the lid 2. In this example, the interior of the cover 2 is designed with an anti-leakage rubber ring; in order to fix the cover 2, further, a plurality of buckles are provided on the outer wall of the cover 2, and four buckles are specifically designed in this example. Correspondingly, a buckle position area matching the buckles is provided on the outer wall of the shell 1, so as to fix the cover 2 on the shell 1.

[0027] In this specific example, the culture dish rack 3 is a four-layer structure, and in a further improvement, a handle 31 is provided at the top of the culture dish rack 3 to facilitate lifting the culture dish rack 3. The culture dish rack 3 is a drawer-type structure, which is pulled out from the side opening to facilitate the placement of culture dishes.

[0028] Further, the culture dish rack 3 is connected to the test tube rack 4. The test tube rack 4 is provided with holes for accommodating test tubes of different sizes, for example, 3-5 holes for inserting the test tubes, and 3 holes are specifically designed in this example.

[0029] Furthermore, the number of grooves is at least 4, which are used to place different reagents. In this specific example, 4 grooves with a width of 2 cm are designed. The first groove is used to fill sodium bicarbonate, citric acid and potassium borocyanide (a separate bag) in a ratio of 2:1:1, and carbon dioxide and hydrogen are produced after adding water; dried palladium particles (palladium catalyst) are added to the second groove, which can catalyze the reaction of hydrogen and oxygen to produce water; oxygen indicator is added to the third groove; calcium chloride dry bag is added to the fourth groove to absorb excess water vapor generated during the reaction in the tank.

[0030] Furthermore, the anaerobic tank of this example is a cylindrical tube, which can be a cylindrical tube with a diameter of 9-20 cm and a height of 15-30 cm. For example, the specification of 9-11 cm is suitable for a bacterial culture dish with a diameter of 6 cm, and the specification of 11-16 cm is suitable for a bacterial culture dish with a diameter of 9 cm. The height of 22 cm is generally suitable for all test tubes, and 15 cm is suitable for short test tubes. Furthermore, for easy handling, a handle can be provided on the outer wall or lid 2 of the anaerobic tank.

[0031] The usage of this anaerobic tank includes:

[0032] 1.Pack sodium bicarbonate, sodium citrate, and potassium borohydride (the first two in one package, the last in a separate package) in a filter paper bag at a ratio of 2:1:1; place the palladium particles in a plastic bag and place them in the groove area. Place them in two grooves, and place the grooves farther apart to prevent the palladium particles from contacting water.

[0033] 2. After inoculating anaerobic bacteria, place the bacterial culture dish to be cultured in the middle drawer-type handle culture dish placement rack, and then place the culture dish placement rack into the culture tank.

[0034] 3. The test tube hole next to it can be used to insert test tubes after inoculating anaerobic bacteria into the test tube liquid culture medium to conduct liquid proliferation experiments of anaerobic bacteria.

[0035] 4. After the culture is prepared, add appropriate amount of water to the groove of the filter paper bag of sodium citrate, sodium bicarbonate and potassium borohydride to cover the filter paper bag to make it react, cover the tank lid, seal the tank body, and use the vacuum interface and vacuum device on the tank body to expel the air in the tank body as much as possible for the first time. The chemical reaction causes a large amount of carbon dioxide and hydrogen to be produced. The palladium catalyst catalyzes the reaction of hydrogen and oxygen to produce water and consume the residual oxygen. The excess carbon dioxide and hydrogen produced are then discharged again by the vacuum device, so that the anaerobic environment in the tank body can be maintained for as long as possible.

[0036] The anaerobic tank in this example has the following advantages: (1) Low cost: easy to make, low material cost, manual preparation, total cost less than 100 yuan. (2) Convenient operation: reasonable design, suitable for different experimental needs, suitable for both solid culture medium and liquid culture medium. (3) High efficiency and stability: through the preparation of hydrogen by common reagents and catalytic consumption of oxygen, combined with a vacuum pumping device, the negative pressure in the tank is maintained under the premise of completing the replacement of the gas in the tank, maintaining the anaerobic state of the culture area.

[0037] The above content is a detailed description of the present application in combination with specific implementation methods, and it cannot be determined that the implementation method of the present application is limited to these descriptions. For ordinary technicians in the technical field of the present application, several simple deductions or substitutions can be made without departing from the basic utility model concept of the present application.

Claims

1. An anaerobic tank, characterized in that: It comprises a housing (1), a cover (2), a culture dish placement rack (3) and a test tube placement rack (4); The shell (1) encloses an anaerobic culture cavity (11) with an opening at the top, and a culture dish placement rack (3) with a multi-layer structure that can be lifted upward is arranged in the anaerobic culture cavity (11). The culture dish placement rack (3) is in a relatively closed state, with an opening on a side facing the side wall of the shell (1), and encloses a relatively closed cavity for accommodating culture dishes with the side wall of the anaerobic culture cavity (11); In the anaerobic culture cavity (11), a test tube placement rack (4) for accommodating test tubes and a plurality of independent grooves are arranged next to the culture dish placement rack (3); The cover (2) matches the top opening of the anaerobic culture cavity (11), and a vacuum extraction interface (21) is provided on the cover (2).

2. The anaerobic tank according to claim 1, characterized in that: The top end of the culture dish placement rack (3) is provided with a handle (31) to facilitate lifting the culture dish placement rack (3).

3. The anaerobic tank according to claim 1, characterized in that: The culture dish placement rack (3) is a drawer-type structure, which can be drawn out from a side opening to facilitate placement of culture dishes.

4. The anaerobic tank according to claim 1, characterized in that: The culture dish placement rack (3) and the test tube placement rack (4) are connected together, and the test tube placement rack (4) is provided with holes for accommodating test tubes of different sizes.

5. The anaerobic tank according to claim 1, characterized in that: The lid (2) is internally provided with an air leakage-proof rubber ring.

6. The anaerobic tank according to claim 1, characterized in that: The number of the grooves is at least 4, which are used to place different reagents.

7. The anaerobic tank according to claim 1, characterized in that: The cover (2) is also provided with a one-way exhaust valve (22).

8. The anaerobic tank according to claim 1, characterized in that: The anaerobic tank is a cylindrical tube with a diameter of 9-20 cm and a height of 15-30 cm.

9. The anaerobic tank according to claim 1, characterized in that: A plurality of buckles are arranged on the outer wall of the cover (2), and correspondingly, a buckle fastening area matching the buckles is arranged on the outer wall of the shell (1), so as to facilitate fixing the cover (2) on the shell (1).

10. The anaerobic tank according to any one of claims 1 to 9, characterized in that: A handle is provided on the outer wall of the anaerobic tank or the cover (2) to facilitate picking up the anaerobic tank.

Images