Sample sampler for preparing stem cells

Abstract

The utility model discloses a sample sampler for preparing stem cells, which comprises a machine body, a liquid storage tank is fixedly mounted on one side of the machine body, a water pump is fixedly mounted on one side of the top of the machine body, a liquid inlet pipe is fixedly mounted at the liquid inlet end of the water pump, and a first liquid outlet pipe is fixedly mounted at the liquid outlet end of the water pump. A second motor is fixedly mounted on the other side of the top of the machine body, a third rotating shaft is fixedly mounted on an output shaft of the second motor, a first bevel gear is fixedly mounted at one end of the third rotating shaft, an insertion pipe is inserted into and rotationally connected to the top of the machine body, and a second bevel gear is fixedly mounted on the outer side of the insertion pipe; centrifugal liquid is stored in the liquid storage tank, the centrifugal liquid is pumped out through the water pump and guided into the insertion pipe through the first liquid outlet pipe, and the insertion pipe can rotate under the action of the second motor so that the centrifugal liquid can be evenly discharged and distributed in blood from the second liquid outlet pipe. Therefore, the mixing effect within the set time is better.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell preparation, in particular to a sample sampler for preparing stem cells. Background Art

[0002] Stem cells are cells with unlimited or immortal self-renewal capabilities and can produce at least one type of highly differentiated daughter cells. The sampling of stem cells plays a major role in the cultivation and research of stem cells. Therefore, we need a stem cell sample collector to collect stem cells. Currently, the collection method is mostly centrifugal separation.

[0003] In the prior art with patent number CN218710337U, a stem cell sample preparation sampler is proposed, which uses a mixing component to mix the collected blood sample with heparin and centrifugal fluid to prevent the blood sample from clotting during the separation process and facilitate centrifugal separation.

[0004] However, although two liquid inlet tubes are provided in the above-mentioned prior art, the centrifuge and blood are not evenly distributed, resulting in poor mixing effect within a given time. Therefore, the present application proposes a sample sampler for preparing stem cells to solve this problem. Utility Model Content

[0005] The purpose of the utility model is to provide a sample sampler for preparing stem cells, so as to solve the problem that although two liquid inlet tubes are provided in the prior art mentioned in the above background technology, the centrifuge and blood are not evenly distributed, resulting in poor mixing effect within a given time.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A sample sampler for preparing stem cells comprises a body, wherein a liquid storage tank is fixedly installed on one side of the body, a water pump is fixedly installed on one side of the top of the body, a liquid inlet pipe is fixedly installed on the liquid inlet end of the water pump, a first liquid outlet pipe is fixedly installed on the liquid outlet end of the water pump, a second motor is fixedly installed on the other side of the top of the body, a third rotating shaft is fixedly installed on the output shaft of the second motor, a first bevel gear is fixedly installed on one end of the third rotating shaft, a cannula is inserted and rotatably connected to the top of the body, a second bevel gear is fixedly installed on the outside of the cannula, a second liquid outlet pipe is symmetrically fixedly installed on both sides of one end of the cannula meshing with the first bevel gear and the second bevel gear, a second rotating shaft is fixedly installed on the bottom end of the cannula, stirring shafts are symmetrically fixedly installed on both sides of the second rotating shaft, a first motor is fixedly installed at a middle position at the bottom of the body, a first rotating shaft is fixedly installed on the output shaft of the first motor, two turntables are arranged inside the body, one end of the first rotating shaft is fixedly connected to the lower turntable, a side wall of the upper turntable is fixedly connected to the body, and a rotating drum is fixedly installed on the outside of the first rotating shaft.

[0008] As a further solution of the utility model: the inner bottom side of the insertion tube is convex in design.

[0009] As a further solution of the utility model: a first liquid infusion tube is fixedly installed on one side of the top of the body, and a second liquid infusion tube is fixedly installed on one side of the liquid storage tank.

[0010] As a further solution of the utility model: a plurality of notches are provided on the two rotating disks, and the two rotating disks are adapted to each other.

[0011] As a further solution of the utility model: a liquid guide groove is fixedly installed inside the body and below the turntable, and a liquid outlet is opened on the liquid guide groove.

[0012] As a further solution of the utility model: a piston cover is fixedly installed on the bottom of one side of the rotating drum, and a closing door is hingedly provided on the front side of the machine body.

[0013] As a further solution of the utility model: a switch is fixedly installed on the machine body, the first motor, the second motor and the water pump are electrically connected to the switch through wires, and the switch is electrically connected to the external power supply through wires.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] 1. The utility model stores the centrifugal liquid in the liquid storage tank, extracts the centrifugal liquid through a water pump and introduces it into the inside of the cannula through a first liquid outlet pipe. The cannula can rotate under the action of a second motor so that the centrifugal liquid is evenly discharged from the second liquid outlet pipe and distributed in the blood, so that the mixing effect is better within a given time.

[0016] 2. The utility model separates stem cells by first mixing centrifuge liquid with blood and centrifuging the mixed liquid, and inserting the pumped pipe into the piston cover and into the interior of the drum, thereby completing the sampling of stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of the utility model.

[0018] Figure 2 It is a top view of the turntable in the utility model.

[0019] Figure 3 It is the front view in the utility model.

[0020] 1. First liquid outlet pipe; 2. Water pump; 3. Liquid inlet pipe; 4. Liquid storage tank; 5. Notch; 6. Liquid guide groove; 7. Rotating drum; 8. Piston cover; 9. First motor; 10. First rotating shaft; 11. Liquid outlet; 12. Turntable; 13. Second rotating shaft; 14. Stirring shaft; 15. Second liquid outlet pipe; 16. Second motor; 17. First bevel gear; 18. Second bevel gear; 19. Insert tube; 20. Closing door; 21. Machine body; 22. Third rotating shaft. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figures 1 to 3In an embodiment of the utility model, a sample sampler for preparing stem cells includes a body 21, a liquid storage tank 4 is fixedly installed on one side of the body 21, a water pump 2 is fixedly installed on one side of the top of the body 21, a liquid inlet pipe 3 is fixedly installed on the liquid inlet end of the water pump 2, a first liquid outlet pipe 1 is fixedly installed on the liquid outlet end of the water pump 2, a second motor 16 is fixedly installed on the other side of the top of the body 21, a third rotating shaft 22 is fixedly installed on the output shaft of the second motor 16, a first bevel gear 17 is fixedly installed on one end of the third rotating shaft 22, a cannula 19 is inserted and rotatably connected to the top of the body 21, a second bevel gear 18 is fixedly installed on the outer side of the cannula 19, and a third bevel gear 19 is fixedly installed on the outer side of the cannula 19. A second liquid outlet pipe 15 is symmetrically fixedly installed on both sides of one end of the insertion tube 19 that meshes with a bevel gear 17 and a second bevel gear 18, a second rotating shaft 13 is fixedly installed on the bottom end of the insertion tube 19, and stirring shafts 14 are symmetrically fixedly installed on both sides of the second rotating shaft 13. A first motor 9 is fixedly installed at the bottom and in the middle of the body 21, and a first rotating shaft 10 is fixedly installed on the output shaft of the first motor 9. Two turntables 12 are arranged inside the body 21, one end of the first rotating shaft 10 is fixedly connected to the lower turntable 12, and the side wall of the upper turntable 12 is fixedly connected to the body 21, and a rotating drum 7 is fixedly installed on the outer side of the first rotating shaft 10.

[0023] The inner bottom side of the cannula 19 is designed to be convex, so that the centrifuge liquid can be discharged more smoothly.

[0024] A first liquid infusion tube is fixedly installed on one side of the top of the body 21 , and a second liquid infusion tube is fixedly installed on one side of the liquid storage tank 4 .

[0025] A plurality of notches 5 are provided on the two rotating disks 12 . The two rotating disks 12 are adapted to each other, and the mixed liquid can be discharged by rotating the lower rotating disk 12 .

[0026] A liquid guiding groove 6 is fixedly installed inside the body 21 and below the rotating disk 12 , and a liquid outlet 11 is formed on the liquid guiding groove 6 .

[0027] A piston cover 8 is fixedly mounted on the bottom of one side of the rotating drum 7, and a closing door 20 is hingedly provided on the front side of the body 21 for taking out the collected stem cells.

[0028] A switch is fixedly mounted on the machine body 21 , and the first motor 9 , the second motor 16 and the water pump 2 are all electrically connected to the switch through wires, and the switch is electrically connected to an external power source through wires.

[0029] The working principle of the utility model is:

[0030] When the utility model is used, blood is added to the inside of the body 21 through the feed port, centrifugal liquid is added to the inside of the liquid storage tank 4, the first liquid outlet pipe 1 is manually supported and inserted into the inside of the cannula 19, the centrifugal liquid is extracted and introduced into the inside of the cannula 19 through the water pump 2, and the second motor 16 is started. The second motor 16 drives the first bevel gear 17 and the second bevel gear 18 to mesh, and the second bevel gear 18 drives the cannula 19 to rotate so that the centrifugal liquid is discharged from one end of the second liquid outlet pipe 15. The second liquid outlet pipe 15 will rotate with the start of the second motor 16, so that the centrifugal liquid is evenly sprinkled into the blood, and the cannula 19 will drive the second motor 16 to rotate. The rotating shaft 13 rotates and then drives the stirring shaft 14 to rotate to fully mix the centrifuge and the blood. After mixing for a period of time, the servo-controlled first motor 9 is started. The first motor 9 controls the lower turntable 12 to rotate so that the notches 5 on the two turntables 12 correspond to each other. At this time, the mixed blood will enter the liquid guide groove 6 from the notch and flow into the inside of the drum 7. After all the blood is released, the output shaft of the first motor 9 is controlled to rotate quickly, so that the drum 7 rotates centrifugally, so that the stem cells can be separated. The closing door 20 is opened and the pipeline is inserted into the piston cover 8 to pump out the stem cells, thereby completing the sampling of the stem cells.

[0031] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A sample collection device for preparing stem cells, comprising a body (21), characterized in that: A liquid storage tank (4) is fixedly mounted on one side of the machine body (21); a water pump (2) is fixedly mounted on one side of the top of the machine body (21); a liquid inlet pipe (3) is fixedly mounted on the liquid inlet end of the water pump (2); a first liquid outlet pipe (1) is fixedly mounted on the liquid outlet end of the water pump (2); a second motor (16) is fixedly mounted on the other side of the top of the machine body (21); a third rotating shaft (22) is fixedly mounted on the output shaft of the second motor (16); a first bevel gear (17) is fixedly mounted on one end of the third rotating shaft (22); a plug (19) is inserted into and rotatably connected to the top of the machine body (21); a second bevel gear (18) is fixedly mounted on the outer side of the plug (19); the first bevel gear (17) and the second bevel gear (18) are connected to each other. ) A second liquid outlet pipe (15) is symmetrically fixedly installed on both sides of one end of the meshing cannula (19), a second rotating shaft (13) is fixedly installed on the bottom end of the cannula (19), stirring shafts (14) are symmetrically fixedly installed on both sides of the second rotating shaft (13), a first motor (9) is fixedly installed at the bottom and in the middle of the machine body (21), the output shaft of the first motor (9) is fixedly installed with a first rotating shaft (10), two rotating disks (12) are arranged inside the machine body (21), one end of the first rotating shaft (10) is fixedly connected to the lower rotating disk (12), the side wall of the upper rotating disk (12) is fixedly connected to the machine body (21), and a rotating drum (7) is fixedly installed on the outer side of the first rotating shaft (10).

2. A sample sampler for preparing stem cells according to claim 1, characterized in that: The inner bottom side of the insertion tube (19) is of convex design.

3. A sample collector for preparing stem cells according to claim 1, characterized in that: A first liquid infusion tube is fixedly mounted on one side of the top of the machine body (21), and a second liquid infusion tube is fixedly mounted on one side of the liquid storage tank (4).

4. A sample collector for preparing stem cells according to claim 1, characterized in that: A plurality of notches (5) are provided on the two rotating disks (12), and the two rotating disks (12) are adapted to each other.

5. The sample collector for preparing stem cells according to claim 1, characterized in that: A liquid guide groove (6) is fixedly installed inside the machine body (21) and below the rotating disk (12), and a liquid outlet (11) is provided on the liquid guide groove (6).

6. The sample collector for preparing stem cells according to claim 1, characterized in that: A piston cover (8) is fixedly mounted on the bottom of one side of the rotating drum (7), and a closing door (20) is hingedly arranged on the front side of the machine body (21).

7. A sample collector for preparing stem cells according to claim 1, characterized in that: A switch is fixedly mounted on the machine body (21); the first motor (9), the second motor (16) and the water pump (2) are all electrically connected to the switch via wires; and the switch is electrically connected to an external power source via wires.

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