Constant-temperature cell culture system

Abstract

The utility model discloses a constant-temperature cell culture system which comprises a cell culture box, a heating fan is arranged on the outer wall of one transverse side of the cell culture box, and a high-temperature protection device is arranged on the inner wall of one transverse side of the cell culture box; the high-temperature protection device comprises an air inlet and a plurality of air guide holes, the air guide holes are arranged into an air guide matrix, and the air inlet is right opposite to the air guide matrix and the air outlet side of the heating fan. According to the utility model, the high-temperature protection device is adopted to guide high-temperature air into the rear part of the cell culture box, so that direct blowing of the high-temperature air is avoided, a small amount of high-temperature air can be directly blown into the cell culture box through the air guide holes, and hot air is uniformly guided into the cell culture box while local high temperature is avoided; therefore, the cell culture environment in the incubator is more stable, and the observed cells are prevented from being influenced by local high temperature.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture systems, relates to a cell culture system for a microscope, and specifically relates to a constant temperature cell culture system. Background Art

[0002] Cell culture refers to a technology that simulates the in vivo environment in vitro to allow cells or biological tissues to grow, reproduce and maintain their main structure and function. In scientific research work that focuses on in vitro cultured cells, it is usually necessary to combine cell culture technology with microscopic imaging technology in order to study the physiological indicators of cells under a normal cell culture environment. Currently, there are commercial cell culture systems that can be used with microscopes.

[0003] The main defects of the existing technology are as follows: First, in order to maintain a normal cell culture environment, air at a certain temperature needs to be introduced into the cell culture system for the microscope. However, when the high-temperature air is blown directly into the cell culture incubator, it is easy to cause local high temperatures in the cell culture incubator. The local high temperatures are easily distributed on the microscope operating table in the cell culture incubator, which makes the observed cells affected by the local high temperatures, resulting in poor cell status, which has a great impact on the experimental results. Second, after the high-temperature air is blown directly into the cell culture incubator, the plate of the cell culture incubator will deform after a long time, which will reduce the airtightness and thermal insulation performance of the cell culture incubator, and then cause the cells to be adversely affected by the external environment. Summary of the invention

[0004] In view of the shortcomings of the prior art, the purpose of the utility model is to provide a constant temperature cell culture system to solve the technical problem in the prior art that high temperature air easily causes local high temperature on the microscope operating table in the cell culture box.

[0005] In order to solve the above technical problems, the utility model adopts the following technical solutions:

[0006] A constant temperature cell culture system, comprising a cell culture box, wherein a heating fan is arranged on an outer wall of one lateral side of the cell culture box, and a high temperature protection device is arranged on an inner wall of one lateral side of the cell culture box;

[0007] The high-temperature protection device includes a first side panel and a second side panel arranged along the vertical direction, and the first side panel and the second side panel are arranged parallel to each other; a top plate of the high-temperature protection device is fixedly arranged at the top end of the first side panel and the second side panel, and a bottom plate of the high-temperature protection device is fixedly arranged at the bottom end of the first side panel and the second side panel; an air inlet is provided on the first side panel, and the air inlet is opposite to the air outlet side of the heating fan; a plurality of air guide holes are provided on the second side panel, and the plurality of air guide holes are arranged into an air guide matrix, and the air inlet is opposite to the air guide matrix.

[0008] The diameter D of the air inlet is 54 mm; the diameter d of the air guide hole is 4 mm; each row of the air guide matrix is ​​provided with 7 air guide holes at equal intervals, and the interval A between adjacent air guide holes in the lateral direction is 1 10mm; each column of the air guide matrix is ​​provided with 6 air guide holes at equal intervals, and the spacing A of adjacent air guide holes in the vertical direction is 2 is 10mm.

[0009] The utility model also has the following technical features:

[0010] The first side plate is provided with two high temperature protection device installation holes; the second side plate is provided with two installation operation holes, and the two installation operation holes are respectively opposite to the two high temperature protection device installation holes.

[0011] The top plate, bottom plate and four side plates of the cell culture box are all three-layer structural plates; the three-layer structural plates are, from inside to outside, an inner heat-resistant layer, a middle air insulation layer and an outer protective layer, the inner heat-resistant layer and the outer protective layer are solid structures, and the middle air insulation layer is a hollow structure.

[0012] The thickness of the inner heat-resistant layer is 4 to 6 mm; the thickness of the intermediate air heat-insulating layer is 2 to 4 mm; and the thickness of the outer protective layer is 1 to 3 mm.

[0013] A support beam is arranged at the edge of the interior of the cell culture box, and the support beam is fixedly connected to the inner heat-resistant layer by screws.

[0014] The top plate, the bottom plate and the four side plates of the cell culture box are fixedly connected by buckles.

[0015] Two operation windows are arranged on the side panels on the longitudinal front side of the cell culture box, and door panels are arranged on the operation windows.

[0016] A microscope mounting port is provided on the bottom plate of the cell culture box, a microscope through hole is provided on the top plate of the cell culture box, the space enclosed by the top plate, the bottom plate and four side plates of the cell culture box is an operation chamber, a microscope is arranged in the operation chamber, the microscope stage is arranged in the microscope mounting port, and the microscope barrel passes through the microscope through hole.

[0017] Compared with the prior art, the utility model has the following technical effects:

[0018] (I) The utility model adopts a high temperature protection device to guide the high temperature air to the rear of the cell culture box, thereby avoiding the direct blowing of the high temperature air. The air guide hole can allow a small amount of high temperature air to be blown directly into the cell culture box, while avoiding local high temperature and uniformly guiding the hot air into the cell culture box, thereby making the cell culture environment in the culture box more stable and avoiding the observed cells from being affected by the local high temperature.

[0019] (II) The utility model adopts a three-layer structural plate to form a cell culture box, which improves the high temperature resistance, heat preservation performance and overall strength of the cell culture box. The cell culture box does not deform after high temperature air is blown into it, ensuring the airtightness, reducing the impact of the external environment on the culture box, and is more sturdy and durable. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Schematic diagram of the overall structure of the constant temperature cell culture system.

[0021] Figure 2 Schematic diagram of the overall structure of the constant temperature cell culture system (with Figure 1 in the opposite direction of projection).

[0022] Figure 3 It is a structural schematic diagram of the high temperature protection device.

[0023] Figure 4 The schematic diagram of the high temperature protection device (with Figure 3 in the opposite direction of projection).

[0024] Figure 5 It is a schematic diagram of the structure of a three-layer structural plate.

[0025] The meanings of the numbers in the figure are: 1-cell culture box, 2-heating fan, 3-high temperature protection device, 4-inner heat-resistant layer, 5-middle air insulation layer, 6-outer protection layer, 7-support beam, 8-clip, 9-operation window, 10-door panel.

[0026] 101 - microscope mounting port, 102 - microscope through hole.

[0027] 301 - first side plate, 302 - second side plate, 303 - top plate of high temperature protection device, 304 - bottom plate of high temperature protection device, 305 - air inlet, 306 - air guide hole, 307 - installation hole of high temperature protection device, 308 - installation operation hole.

[0028] The specific contents of the utility model are further explained in detail below in conjunction with the embodiments. DETAILED DESCRIPTION

[0029] It should be noted that, unless otherwise specified, all components in the present invention are components known in the art.

[0030] The following are specific embodiments of the present invention. It should be noted that the present invention is not limited to the following specific embodiments, and all equivalent changes made on the basis of the technical solution of the present application fall within the protection scope of the present invention.

[0031] Example:

[0032] This embodiment provides a constant temperature cell culture system, such as Figure 1 and Figure 2 As shown, it includes a cell culture box 1, a heating fan 2 is arranged on the outer wall of one lateral side of the cell culture box 1, and a high temperature protection device 3 is arranged on the inner wall of one lateral side of the cell culture box 1;

[0033] like Figure 3 and Figure 4 As shown, the high temperature protection device 3 includes a first side plate 301 and a second side plate 302 arranged along the vertical direction, and the first side plate 301 and the second side plate 302 are arranged parallel to each other; a high temperature protection device top plate 303 is fixedly arranged at the top of the first side plate 301 and the second side plate 302, and a high temperature protection device bottom plate 304 is fixedly arranged at the bottom of the first side plate 301 and the second side plate 302; an air inlet 305 is opened on the first side plate 301, and the air inlet 305 is directly opposite to the air outlet side of the heating fan 2; a plurality of air guide holes 306 are opened on the second side plate 302, and the plurality of air guide holes 306 are arranged in an air guide matrix, and the air inlet 305 is directly opposite to the air guide matrix;

[0034] The diameter D of the air inlet 305 is 54 mm; the diameter d of the air guide hole 306 is 4 mm; each row of the air guide matrix is ​​provided with 7 air guide holes 306 at equal intervals, and the interval A between adjacent air guide holes 306 in the lateral direction is 1 Each column of the air guide matrix is ​​provided with 6 air guide holes 306 at equal intervals, and the spacing A of adjacent air guide holes 306 in the vertical direction is 2 is 10mm.

[0035] In this embodiment, the high temperature protection device 3 is arranged on the inner heat-resistant layer 4. The high temperature protection device 3 is made of high temperature resistant photosensitive resin and alloy material. The high temperature protection device 3 can introduce high temperature air to the rear of the cell culture box 1, so as to avoid direct blowing of high temperature air; a number of air guide holes 306 arranged according to design and calculation are arranged on the front, which can make a small amount of high temperature air blow directly into the cell culture box 1, and make the temperature more uniform while avoiding local high temperature, so as to achieve the purpose of uniformly introducing the hot air blown by the heating fan 2 into the cell culture box 1, making the cell culture environment in the culture box more stable, and avoiding the damage of cells to the high temperature caused by direct blowing of hot air into the culture box.

[0036] As a specific solution of this embodiment, Figure 3 and Figure 4 As shown, two high temperature protection device installation holes 307 are provided on the first side plate 301 ; two installation operation holes 308 are provided on the second side plate 302 , and the two installation operation holes 308 are respectively opposite to the two high temperature protection device installation holes 307 .

[0037] In this embodiment, the high temperature protection device installation hole 307 is convenient for firmly connecting the high temperature protection device 3 to the cell culture box 1 by screws; the installation operation hole 308 is convenient for installing screws in a narrow space.

[0038] As a specific solution of this embodiment, Figure 5 As shown, the top plate, bottom plate and four side plates of the cell culture box 1 are all three-layer structural plates; the three-layer structural plates are, from inside to outside, an inner heat-resistant layer 4, a middle air insulation layer 5 and an outer protective layer 6, the inner heat-resistant layer 4 and the outer protective layer 6 are solid structures, and the middle air insulation layer 5 is a hollow structure.

[0039] In this embodiment, the inner heat-resistant layer 4 and the middle air insulation layer 5 are fixedly connected by screws and adhered by acrylic glue, and the middle air insulation layer 5 and the outer protective layer 6 are adhered by acrylic glue; the inner heat-resistant layer 4 is arranged at the innermost side of the incubator, and it has the characteristics of high temperature resistance, and it can not be deformed when the high-temperature air blown by the heating fan is blown in, so as to ensure the airtightness of the incubator. The middle air insulation layer 5 is arranged between the inner heat-resistant layer 4 and the outer protective layer 6, and it has the function of heat preservation, so as to improve the thermal efficiency and reduce the influence of the external environment on the incubator. The outer protective layer 6 is arranged at the outermost side of the incubator, and it has the characteristics of being strong and scratch-resistant, so that the incubator is more strong and durable and the structure is maintained stable.

[0040] As a specific solution of this embodiment, the inner heat-resistant layer 4 is made of high-temperature resistant acrylic material or polyphenylene sulfide plastic, with a thickness of 4 to 6 mm, with 4 mm being optimal; the middle air insulation layer 5 is made of acrylic material, with a thickness of 2 to 4 mm, with 2 mm being optimal; the outer protective layer 6 is made of acrylic material, with a thickness of 1 to 3 mm, with 2 mm being optimal.

[0041] As a specific solution of this embodiment, Figure 5 As shown, a support beam 7 is provided at the edge of the interior of the cell culture box 1 , and the support beam 7 is fixedly connected to the inner heat-resistant layer 4 by screws. In this embodiment, the support beam 7 is made of acrylic material, constituting a stable basic skeleton structure of the cell culture box 1 .

[0042] As a specific solution of this embodiment, Figure 1 and Figure 2 As shown, the top plate, the bottom plate and the four side plates of the cell culture box 1 are fixedly connected by buckles 8. In this embodiment, the buckles 8 are convenient for flexible disassembly and installation, while taking into account the sealing performance.

[0043] As a specific solution of this embodiment, two operation windows 9 are provided on the side panels of the longitudinal front side of the cell culture box 1, and a door panel 10 is provided on the operation window 9. In this embodiment, after the door panel 10 is opened, the operation can be performed in the cell culture box 1 through the operation window 9.

[0044] As a specific solution of this embodiment, Figure 1 and Figure 2 As shown, a microscope mounting port 101 is provided on the bottom plate of the cell culture box 1, a microscope through hole 102 is provided on the top plate of the cell culture box 1, and a space enclosed by the top plate, the bottom plate and the four side plates of the cell culture box 1 is an operation chamber, in which a microscope is arranged, and the stage of the microscope is arranged in the microscope mounting port 101, and the lens barrel of the microscope passes through the microscope through hole 102.

[0045] As a specific solution of this embodiment, a culture box is arranged on the stage of the microscope, and a temperature and humidity detector is arranged on the inner wall of the culture box. In this embodiment, the culture box is a culture box made of photosensitive resin material and glass known in the prior art, and is used to place a cell culture container; the temperature and humidity detector is used to monitor the temperature and humidity in the cell culture box 1 in real time.

[0046] As a specific solution of this embodiment, the heating fan 2 and the temperature and humidity detector are both connected to the temperature and humidity control system. In this embodiment, the temperature and humidity control system is a temperature and humidity control system known in the prior art, which includes a temperature and humidity control device, an air inlet, a power switch and a power line interface, a mixed steam output interface, a temperature and humidity detector interface, a heating fan interface, an air flow meter and a steam heating bottle, and the temperature and humidity control device is made of photosensitive resin material and plastic. The air inlet is connected to the input end of the gas flow meter through a pipeline, the output end of the gas flow meter is connected to the steam heating bottle through a pipeline, the steam heating bottle is connected to the mixed steam output interface through a pipeline, the mixed steam output interface is set on the temperature and humidity control device, and the mixed steam output interface is connected to the culture box through a pipeline; the temperature and humidity detector interface is used to connect the temperature and humidity detector; the heating fan interface is located below the temperature and humidity detector interface, and is used to connect the heating fan 2.

[0047] In this embodiment, during conventional cell culture, the air inlet is connected to filtered air and 99.999% CO 2 The mixing ratio of the two gases is controlled by a gas flow meter to fully meet the culture environment requirements of different cells. The mixed gas is transported to the steam heating bottle, and steam is generated by water in the steam heating bottle. Steam is mixed with the mixed gas and then transported to the mixed steam output interface through a pipeline. The mixed steam output interface transports the mixed steam to the culture box in the cell culture incubator 1 through a pipeline.

[0048] As a specific scheme of this embodiment, the temperature and humidity control device is provided with a temperature adjustment button, an incubation box temperature display screen, an incubation box humidity display screen, a cooling fan, an incubation box back cover, a setting display screen, and reinforcing ribs. The temperature adjustment button is electrically connected to the steam heating bottle, the heating fan interface, and the cooling fan. The incubation box temperature display screen, the incubation box humidity display, and the setting display screen are electrically connected to the temperature and humidity detector interface. The incubation box back cover is provided at the rear of the temperature and humidity control device, and the incubation box back cover is provided with reinforcing ribs.

[0049] In this embodiment, the required cell culture parameters are set by the temperature adjustment button, and the temperature and humidity control device provides the required temperature and humidity of the cell culture box 1. The culture box temperature display screen and the culture box humidity display screen facilitate observation of real-time temperature and humidity changes.

[0050] The working process of the utility model is as follows:

[0051] The top plate, bottom plate and four side plates of the cell culture box 1 are assembled and fixed with buckles 8 to keep the cell culture box 1 airtight. The heating fan 2 is turned on, and the high-temperature air enters the high-temperature protection device 3 from the air inlet 305, and is dispersed through the air guide holes 306 and then blown into the cell culture box 1, thereby achieving uniform distribution of hot air and avoiding local high temperature.

Claims

1. A constant temperature cell culture system, It is characterized in that It comprises a cell culture box (1), wherein a heating fan (2) is arranged on an outer wall of one lateral side of the cell culture box (1), and a high temperature protection device (3) is arranged on an inner wall of one lateral side of the cell culture box (1); The high temperature protection device (3) comprises a first side plate (301) and a second side plate (302) arranged along the vertical direction, the first side plate (301) and the second side plate (302) being arranged parallel to each other; a top plate (303) of the high temperature protection device is fixedly arranged at the top of the first side plate (301) and the second side plate (302), and a bottom plate (304) of the high temperature protection device is fixedly arranged at the bottom of the first side plate (301) and the second side plate (302); an air inlet (305) is provided on the first side plate (301), and the air inlet (305) is directly opposite to the air outlet side of the heating fan (2); a plurality of air guide holes (306) are provided on the second side plate (302), and the plurality of air guide holes (306) are arranged in an air guide matrix, and the air inlet (305) is directly opposite to the air guide matrix; The diameter D of the air inlet (305) is 54 mm; the diameter d of the air guide hole (306) is 4 mm; each row of the air guide matrix is ​​provided with 7 air guide holes (306) at equal intervals, and the interval A between adjacent air guide holes (306) in the lateral direction is 1 Each column of the air guide matrix is ​​provided with 6 air guide holes (306) at equal intervals, and the spacing A between adjacent air guide holes (306) in the vertical direction is 2 is 10mm.

2. The constant temperature cell culture system according to claim 1, It is characterized in that The first side plate (301) is provided with two high temperature protection device installation holes (307); the second side plate (302) is provided with two installation operation holes (308), and the two installation operation holes (308) are respectively opposite to the two high temperature protection device installation holes (307).

3. The constant temperature cell culture system according to claim 1, It is characterized in that The top plate, bottom plate and four side plates of the cell culture box (1) are all three-layer structural plates; the three-layer structural plates are, from inside to outside, an inner heat-resistant layer (4), an intermediate air insulation layer (5) and an outer protective layer (6); the inner heat-resistant layer (4) and the outer protective layer (6) are solid structures, and the intermediate air insulation layer (5) is a hollow structure.

4. The constant temperature cell culture system according to claim 3, It is characterized in that The thickness of the inner heat-resistant layer (4) is 4 to 6 mm; the thickness of the intermediate air heat-insulating layer (5) is 2 to 4 mm; and the thickness of the outer protective layer (6) is 1 to 3 mm.

5. The constant temperature cell culture system according to claim 1, It is characterized in that A support beam (7) is provided at the inner edge of the cell culture box (1), and the support beam (7) is fixedly connected to the inner heat-resistant layer (4) by means of screws.

6. The constant temperature cell culture system according to claim 1, It is characterized in that The top plate, bottom plate and four side plates of the cell culture box (1) are fixedly connected via buckles (8).

7. The constant temperature cell culture system according to claim 1, It is characterized in that Two operation windows (9) are provided on the side panels on the longitudinal front side of the cell culture box (1), and door panels (10) are provided on the operation windows (9).

8. The constant temperature cell culture system according to claim 1, It is characterized in that A microscope mounting opening (101) is provided on the bottom plate of the cell culture box (1), a microscope through hole (102) is provided on the top plate of the cell culture box (1), and a space enclosed by the top plate, the bottom plate and the four side plates of the cell culture box (1) is an operation chamber, in which a microscope is arranged, the stage of the microscope is arranged in the microscope mounting opening (101), and the lens barrel of the microscope passes through the microscope through hole (102).

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