High-efficiency fluid particle fluorescence signal collection lens and fluorescence signal collection system

Abstract

The utility model belongs to the technical field of fluorescence collection, and particularly relates to an efficient fluid particle fluorescence signal collection lens which comprises a box body, a fluorescence emitter used for providing fluorescence beams is arranged in the middle of the box body, and a reflection array mirror used for enhancing reflection of the fluorescence beams is arranged at one end in the box body. A refraction array focusing assembly used for refraction focusing of fluorescent light beams is arranged at the other end in the box body; a reflection array mirror is arranged at one end in a box body, and a refraction array focusing assembly is arranged at the other end in the box body, so that the reflection array mirror reflects a fluorescent light beam emitted by a fluorescent emitter through reflection, and the fluorescent light beam is enhanced through the reflection array mirror; and the focusing assembly is used for refracting and focusing the enhanced fluorescent light beam, so that the intensity of the fluorescent signal is increased, and the sensitivity, the signal collection efficiency and the signal-to-noise ratio of the fluorescent signal collection lens are improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of fluorescence collection, and in particular relates to a high-efficiency fluid particle fluorescence signal collection lens and a fluorescence signal collection system. Background Art

[0002] Flow cytometer, also known as fluorescence-activated cell sorter, includes: cell flow system, optical path, circuit, control system and other components; cells can generate specific fluorescence signals when passing through the laser beam of the instrument, which are then collected by the fluorescence signal collection lens, and further decomposed into corresponding channels through the spectrometer module; the fluorescence signal collection lens is the core component of the flow cytometer. For the performance of the instrument, the stronger the fluorescence signal of the fluorescence signal collection lens, the better the detection performance, the higher the sensitivity, and the higher the signal-to-noise ratio.

[0003] However, the existing fluorescence collection lens uses a reflector to reflect and increase the light beam, and uses a convex lens to focus the light beam. However, this method causes insufficient focusing intensity of the fluorescent light beam, and it is difficult to meet the requirements of detection performance, sensitivity and signal-to-noise ratio of the fluorescent signal.

[0004] Based on this, a fluorescence signal collection lens consisting of a reflection array mirror and a refraction array focusing component is designed. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model provides an efficient fluid particle fluorescence signal collection lens and a fluorescence signal collection system. The specific technical scheme is as follows:

[0006] A high-efficiency fluid particle fluorescence signal collection lens comprises a box body, a fluorescence emitter for providing a fluorescence light beam is arranged in the middle of the box body, a reflection array mirror for enhancing the reflection of the fluorescence light beam is arranged at one end inside the box body, and a refraction array focusing component for refracting and focusing the fluorescence light beam is arranged at the other end inside the box body.

[0007] Preferably, the refractive array focusing assembly consists of a convex lens and refractive micro-facets, and the refractive micro-facets are arranged on the outer ring of the convex lens.

[0008] Preferably, the refractive array focusing assembly is composed of refractive micro-facets.

[0009] Preferably, the reflective array mirror is composed of reflective micro-facets, the reflective micro-facets and refractive micro-facets adopt a stepped ring structure, and the refractive micro-facets are a refractive array mirror.

[0010] Preferably, the radial period of the refractive array and the reflective array is a fixed unit length.

[0011] Preferably, the refractive micro-facet is a plane or a curved surface.

[0012] Preferably, the reflective array and refractive array focusing components are embedded.

[0013] A high-efficiency fluid particle fluorescence signal collection system, including a high-efficiency fluid particle fluorescence signal collection lens, the fluorescence signal collection system comprising:

[0014] A fluorescent emitter, the fluorescent emitter is located inside the box and is used to emit fluorescent light;

[0015] A reflective micro-facet, the reflective micro-facet is located at one end of the box, and the reflective micro-facet is used to reflect fluorescence;

[0016] A refractive micro-facet, wherein the refractive micro-facet is located in the refractive array focusing component and is used for refracting fluorescence;

[0017] A convex lens is located in the refractive array focusing assembly and is used to converge fluorescence.

[0018] Preferably, the fluorescence signal collection system complies with the following formula:

[0019]

[0020] By adjusting the near-field phase of each array element, the final far-field energy can be changed.

[0021] Compared with the prior art, the beneficial effects of the utility model are:

[0022] 1. A reflective array mirror is provided at one end of the box, and a refractive array focusing component is provided at the other end of the box, so that the reflective array mirror reflects the fluorescent light beam emitted by the fluorescent emitter by reflection, and the fluorescent light beam is enhanced by the reflective array mirror, and the refractive array focusing component is used to refract and focus the enhanced fluorescent light beam, thereby increasing the intensity of the fluorescent signal, thereby improving the sensitivity of the fluorescent signal collection lens, the signal collection efficiency, and the signal-to-noise ratio.

[0023] 2. The refractive array focusing component is composed of a convex lens and a refractive micro-facet, and the refractive micro-facet is arranged on the outer ring of the convex lens, so that the signal collection efficiency is improved.

[0024] 3. The reflective array mirror is composed of reflective micro-facets. The reflective micro-facets and refractive micro-facets adopt a stepped ring structure. The refractive micro-facets are refractive array mirrors, so that the reflection angle in each direction can be accurately controlled.

[0025] 4. By using the radial period of the array as a fixed unit length, the design can also be optimized according to the energy distribution of the fluorescent molecule radiation field, making the device more sensitive and the signal collection efficiency faster.

[0026] 5. By refracting the micro-facets into flat or curved surfaces, the use of materials is reduced while retaining the energy collection function of the optical system.

[0027] 6. The reflective array and the refractive array focusing components are embedded, which is easy to process, reduces the size of the lens, and reduces the cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the three-dimensional structure of the first embodiment of the present utility model;

[0029] Figure 2 This is a schematic diagram of the three-dimensional structure of the second embodiment of the present utility model;

[0030] Figure 3 Schematic diagram of the reflective micro-facet structure in the utility model;

[0031] Figure 4 This is a schematic diagram of the structure of a refractive array focusing assembly in Example 1 of the utility model;

[0032] Figure 5 This is a schematic diagram of the structure of the refractive array focusing component in Example 2 of the present utility model.

[0033] Figure numerals: 3, reflective micro-facet; 4, convex lens; 5, refractive micro-facet; 6, box; 7, fluorescent emitter. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present utility model are described below.

[0035] Embodiment 1:

[0036] See also Figure 1 , Figure 3 and Figure 4 The utility model provides a technical solution: an efficient fluid particle fluorescence signal collection lens, including a box body 6, a fluorescence emitter 7 for providing a fluorescence light beam is arranged in the middle of the box body 6, a reflection array mirror for enhancing the reflection of the fluorescence light beam is arranged at one end inside the box body 6, and a refraction array focusing component for refracting and focusing the fluorescence light beam is arranged at the other end inside the box body 6.

[0037] In this embodiment, a reflective array mirror is provided at one end of the box body 6, and a refractive array focusing component is provided at the other end of the box body 6, so that the reflective array mirror reflects the fluorescent light beam emitted by the fluorescent emitter 7 by reflection, and the fluorescent light beam is enhanced by the reflective array mirror, and the refractive array focusing component is used to refract and focus the enhanced fluorescent light beam, thereby increasing the intensity of the fluorescent signal, thereby improving the sensitivity of the fluorescent signal collection lens, the signal collection efficiency, and the signal-to-noise ratio.

[0038] Specifically, the refractive array focusing component is composed of a convex lens 4 and refractive micro-facets 5 , and the refractive micro-facets 5 are arranged on the outer ring of the convex lens 4 .

[0039] In this embodiment, the refractive array focusing component is composed of a convex lens 4 and refractive micro-facets 5, and the refractive micro-facets 5 are arranged on the outer ring of the convex lens 4, so that the signal collection efficiency is improved.

[0040] Specifically, the reflective array mirror is composed of reflective micro-facets 3. The reflective micro-facets 3 and the refractive micro-facets 5 adopt a stepped ring structure, and the refractive micro-facets 5 are a refractive array mirror.

[0041] In this embodiment, the reflective array mirror is composed of reflective micro-facets 3, the reflective micro-facets 3 and the refractive micro-facets 5 adopt a stepped ring structure, and the refractive micro-facets 5 are a refractive array mirror, so that the reflection angle in each direction can be accurately controlled.

[0042] Specifically, the radial period of the refractive array and the reflective array is a fixed unit length.

[0043] In this embodiment, the radial period of the array is a fixed unit length, and the design can also be optimized according to the energy distribution of the fluorescent molecule radiation field, so that the device is more sensitive and the signal collection efficiency is faster.

[0044] Specifically, the refractive micro-facet 5 is a plane or a curved surface.

[0045] In this embodiment, the refractive micro-facet 5 is made flat or curved, thereby reducing the use of materials while retaining the energy collection function of the optical system.

[0046] Specifically, the reflective array and the refractive array focusing components are embedded.

[0047] In this embodiment, the reflective array and the refractive array focusing components are embedded, which is easy to process, reduces the size of the lens, and reduces the cost.

[0048] A high-efficiency fluid particle fluorescence signal collection system, including a high-efficiency fluid particle fluorescence signal collection lens, the fluorescence signal collection system includes:

[0049] A fluorescent emitter 7, the fluorescent emitter 7 is located inside the box 6, and the fluorescent emitter 7 is used to emit fluorescent light;

[0050] A reflective micro-facet 3, wherein the reflective micro-facet 3 is located at one end of the box 6;

[0051] A refractive micro-facet 5, wherein the refractive micro-facet 5 is located in the refractive array focusing component, and the refractive micro-facet 5 is used for refracting fluorescence;

[0052] Convex lens 4, which is located in the refraction array focusing assembly and is used to converge fluorescence.

[0053] In this embodiment, through the fluorescent emitter 7, the fluorescent emitter 7 is located inside the box 6, and the fluorescent emitter 7 is used to emit fluorescence to ensure the correct collection of fluorescence;

[0054] By means of the reflective micro-facet 3, the reflective micro-facet 3 is located at one end of the box 6, and the reflective micro-facet 3 is used to reflect the fluorescence, so that the fluorescence can be correctly reflected onto the refractive array focusing component.

[0055] Through the refractive micro-facet 5, the refractive micro-facet 5 is located in the refractive array focusing component, and the refractive micro-facet 5 is used to refract fluorescence: the convex lens 4 is located in the refractive array focusing component, and the convex lens 4 is used to converge fluorescence, and through the refractive micro-facet 5 and the convex lens 4, the reflected fluorescent molecules are collected.

[0056] Specifically, the fluorescence signal collection system conforms to the following formula:

[0057]

[0058] By adjusting the near-field phase of each array element, the final far-field energy can be changed.

[0059] In this embodiment, the above formula is used to improve the collection efficiency of fluorescent radiation and the signal-to-noise ratio.

[0060] The working principle and usage process of this embodiment are as follows: when the fluorescent emitter 7 emits the fluorescent molecules, the fluorescent molecules are then reflected to the refractive array focusing component through the reflective micro-facet 3, and then the refractive micro-facet 5 of the refractive array focusing component cooperates with the convex lens 4 to focus the reflected fluorescent energy to complete the focusing of the fluorescence.

[0061] Embodiment 2:

[0062] See also Figure 2 , Figure 3 and Figure 5 The utility model provides a technical solution: an efficient fluid particle fluorescence signal collection lens, including a box body 6, a fluorescence emitter 7 for providing a fluorescence light beam is arranged in the middle of the box body 6, a reflection array mirror for enhancing the reflection of the fluorescence light beam is arranged at one end inside the box body 6, and a refraction array focusing component for refracting and focusing the fluorescence light beam is arranged at the other end inside the box body 6.

[0063] In this embodiment, a reflective array mirror is provided at one end of the box body 6, and a refractive array focusing component is provided at the other end of the box body 6, so that the reflective array mirror reflects the fluorescent light beam emitted by the fluorescent emitter 7 by reflection, and the fluorescent light beam is enhanced by the reflective array mirror, and the refractive array focusing component is used to refract and focus the enhanced fluorescent light beam, thereby increasing the intensity of the fluorescent signal, thereby improving the sensitivity of the fluorescent signal collection lens, the signal collection efficiency, and the signal-to-noise ratio.

[0064] Specifically, the refractive array focusing component is composed of refractive micro-facets 5.

[0065] In this embodiment, the refractive array focusing components are entirely composed of refractive micro-facets 5, which facilitates overall processing.

[0066] Specifically, the reflective array mirror is composed of reflective micro-facets 3. The reflective micro-facets 3 and the refractive micro-facets 5 adopt a stepped ring structure, and the refractive micro-facets 5 are a refractive array mirror.

[0067] In this embodiment, the reflective array mirror is composed of reflective micro-facets 3, the reflective micro-facets 3 and the refractive micro-facets 5 adopt a stepped ring structure, and the refractive micro-facets 5 are a refractive array mirror, so that the reflection angle in each direction can be accurately controlled.

[0068] Specifically, the radial period of the refractive array and the reflective array is a fixed unit length.

[0069] In this embodiment, the radial period of the array is a fixed unit length, and the design can also be optimized according to the energy distribution of the fluorescent molecule radiation field, so that the device is more sensitive and the signal collection efficiency is faster.

[0070] Specifically, the refractive micro-facet 5 is a plane or a curved surface.

[0071] In this embodiment, the refractive micro-facet 5 is made flat or curved, thereby reducing the use of materials while retaining the energy collection function of the optical system.

[0072] Specifically, the reflective array and the refractive array focusing components are embedded.

[0073] In this embodiment, the reflective array and the refractive array focusing components are embedded, which is easy to process, reduces the size of the lens, and reduces the cost.

[0074] Specifically, a high-efficiency fluid particle fluorescence signal collection system includes a high-efficiency fluid particle fluorescence signal collection lens, and the fluorescence signal collection system includes:

[0075] A fluorescent emitter 7, the fluorescent emitter 7 is located inside the box 6, and the fluorescent emitter 7 is used to emit fluorescent light;

[0076] A reflective micro-facet 3, which is located at one end of the box 6 and is used to reflect fluorescence;

[0077] The refractive micro-facet 5 is located in the refractive array focusing component, and the refractive micro-facet 5 is used for refracting fluorescence.

[0078] In this embodiment, through the fluorescent emitter 7, the fluorescent emitter 7 is located inside the box 6, and the fluorescent emitter 7 is used to emit fluorescence to ensure the correct collection of fluorescence;

[0079] Through the reflective micro-facet 3, the reflective micro-facet 3 is located at one end of the box 6, and the reflective micro-facet 3 is used to reflect the fluorescence, so that the fluorescence can be correctly reflected onto the refractive array focusing component;

[0080] The refractive micro-facet 5 is located in the refractive array focusing component, and the refractive micro-facet 5 is used to refract fluorescence: the reflected fluorescent molecules are collected by the refractive micro-facet 5.

[0081] Specifically, the fluorescence signal collection system conforms to the following formula:

[0082]

[0083] By adjusting the near-field phase of each array element, the final far-field energy can be changed.

[0084] In this embodiment, the above formula is used to improve the collection efficiency of fluorescent radiation and the signal-to-noise ratio.

[0085] The working principle and use process of this embodiment are as follows: when the fluorescent emitter 7 emits the fluorescent molecules, the fluorescent molecules are then reflected to the refractive array focusing component through the reflective micro-facet 3, and then the reflected fluorescent energy is focused by the refractive micro-facet 5 of the refractive array focusing component to complete the focusing of the fluorescence.

[0086] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A high-efficiency fluid particle fluorescence signal collection lens, comprising a housing (6), Features: A fluorescent emitter (7) for providing a fluorescent light beam is arranged in the middle of the box (6), a reflective array mirror for enhancing the reflection of the fluorescent light beam is arranged at one end inside the box (6), and a refraction array focusing component for refracting and focusing the fluorescent light beam is arranged at the other end inside the box (6).

2. The high-efficiency fluid particle fluorescence signal collection lens according to claim 1, Features: The refractive array focusing component is composed of a convex lens (4) and a refractive micro-facet element (5), and the refractive micro-facet element (5) is arranged on the outer ring of the convex lens (4).

3. The high-efficiency fluid particle fluorescence signal collection lens according to claim 1, Features: The refractive array focusing component is composed of refractive micro-facets (5).

4. The high-efficiency fluid particle fluorescence signal collection lens according to claim 2 or 3, Features: The reflective array mirror is composed of reflective micro-facets (3), the reflective micro-facets (3) and the refractive micro-facets (5) adopt a stepped ring structure, and the refractive micro-facets (5) are a refractive array mirror.

5. The high-efficiency fluid particle fluorescence signal collection lens according to claim 4, Features: The radial period of the refractive array and the reflective array is a fixed unit length.

6. The high-efficiency fluid particle fluorescence signal collection lens according to claim 4, Features: The refractive micro-facet (5) is a plane or a curved surface.

7. The high-efficiency fluid particle fluorescence signal collection lens according to claim 1, Features: The reflective array and refractive array focusing components are embedded.

8. A high-efficiency fluid particle fluorescence signal collection system, comprising the high-efficiency fluid particle fluorescence signal collection lens described in any one of 1 to 6, Features: The fluorescence signal collection system comprises: A fluorescent emitter (7), the fluorescent emitter (7) is located inside the box (6), and the fluorescent emitter (7) is used to emit fluorescent light; A reflective micro-facet (3), the reflective micro-facet (3) being located at one end of the box (6), and the reflective micro-facet (3) being used to reflect fluorescence; A refractive micro-facet (5), wherein the refractive micro-facet (5) is located in the refractive array focusing component, and the refractive micro-facet (5) is used to refract fluorescence; A convex lens (4), wherein the convex lens (4) is located in the refractive array focusing component, and the convex lens (4) is used to converge fluorescence.

9. The high-efficiency fluid particle fluorescence signal collection system according to claim 8, Features: The fluorescence signal collection system complies with the following formula: By adjusting the near-field phase of each array element, the final far-field energy can be changed.

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