Sample placing device and extractor comprising same

Abstract

The utility model provides a sample placing device and an extractor comprising the same, the sample placing device comprises an objective table, a sample containing seat and a refrigeration mechanism, the sample containing seat is arranged on the objective table and is used for placing a sample solution, the refrigeration mechanism is arranged below the sample containing seat, and the sample containing seat is used for placing the sample solution. A magnetic mechanism is arranged between the refrigeration mechanism and the sample accommodating seat, can move up and down relative to the sample accommodating seat, and can attract magnetic beads in the sample solution. By arranging the magnetic mechanism, the device can attract magnetic beads in a sample solution, the magnetic beads can be conveniently separated from the sample solution, and whether the magnetic beads are attracted or not can be adjusted according to needs.

Description

Technical Field

[0001] The utility model relates to a sample placing device and an extracting instrument comprising the same. Background Art

[0002] In the process of purifying biological molecules, various liquids need to be transferred, which requires the use of a pipette. When transferring liquids, the pipette needs to use a pipette to absorb the liquid and transfer the absorbed liquid to a predetermined position. Existing operations such as cell and exosome separation, nucleic acid extraction, target identification and metabolic property research of biological drugs, protein purification and immunochromatography usually use magnetic beads combined with drugs, proteins, enzymes, antibodies or nucleic acids for purification. After purification, the specific substances adsorbed on the magnetic beads need to be retained. Magnets are usually used to adsorb magnetic beads on the outer wall of the pipette. However, for the separation of magnetic beads and biological molecules, the magnetic beads are usually sucked away after elution. This separation method is relatively inconvenient and the separation effect is not good. Utility Model Content

[0003] The technical problem to be solved by the utility model is to overcome the above-mentioned defects in the prior art and provide a sample placement device and an extraction instrument comprising the same.

[0004] The utility model solves the above technical problems through the following technical solutions:

[0005] The utility model provides a sample placement device, comprising a stage, a sample holding seat, and a refrigeration mechanism. The sample holding seat is arranged on the stage, the sample holding seat is used to place a sample solution, and the refrigeration mechanism is arranged below the sample holding seat.

[0006] A magnetic mechanism is provided between the refrigeration mechanism and the sample holding seat. The magnetic mechanism is configured to be able to move up and down relative to the sample holding seat, and the magnetic mechanism is configured to be able to attract magnetic beads in the sample solution.

[0007] In the present technical solution, the design of the sample holder enables the sample solution to be conveniently placed on the stage, providing good sample fixing and supporting functions. The refrigeration mechanism is located below the sample holder, which can refrigerate the sample to ensure that the sample is processed or stored at an appropriate temperature. The setting of the magnetic mechanism enables the device to attract the magnetic beads in the sample solution, which is convenient for separation from the sample liquid. When it is necessary to adsorb the magnetic beads in the sample, the magnetic mechanism can move up and down relative to the sample holder, and this adsorption operation can conveniently achieve the separation, aggregation or other processing requirements of the magnetic beads. When it is not necessary to adsorb the magnetic beads, the magnetic mechanism can be moved to a position away from the sample holder, so that the magnetic beads are no longer affected by the magnetic force, thereby stopping adsorption. Such operational flexibility makes it possible to control the adsorption state of the magnetic beads according to the needs of the experimental process or processing steps, thereby improving the freedom and controllability of the operation.

[0008] Preferably, the sample placement device further comprises a lifting mechanism, the lifting mechanism comprises a fixing frame and a support member, the fixing frame is provided with a guide column, the support member is slidably matched with the guide column, and the magnetic mechanism is arranged on the support member; the lifting mechanism further comprises a motor and a screw rod, the motor is drivingly connected with the screw rod, and the support member is provided with a screw rod sliding sleeve portion threadedly connected with the screw rod;

[0009] The axes of the screw rod and the guide column are parallel and extend along the moving direction of the magnetic mechanism.

[0010] In this technical solution, the lifting mechanism can realize accurate positioning and control of the support member and the magnetic mechanism. The rotation of the motor drives the screw to rotate, thereby causing the support member to move up and down, realizing the lifting operation of the magnetic mechanism.

[0011] Preferably, the screw rod is connected to the output shaft of the motor via a transmission belt.

[0012] In the technical solution, in the sample placement device, the screw rod and the output shaft of the motor are connected by a transmission belt. This transmission connection method can use a transmission belt (such as a belt, a toothed belt, etc.) to transmit the rotational motion of the motor to the screw rod. The screw rod is connected to the output shaft of the motor through the transmission belt, so that the motor can transmit the rotational torque to the screw rod. The power of the motor can be effectively transmitted to the screw rod, realizing the lifting and lowering motion control of the support member and the magnetic mechanism.

[0013] Preferably, the fixing frame comprises an upper frame and a lower frame, the upper frame and the lower frame are fixedly connected via a plurality of guide posts, and the supporting member is provided with a sliding sleeve seat which slides in one-to-one correspondence with the plurality of guide posts.

[0014] In this technical solution, the combination of the guide column and the sliding sleeve seat can realize reliable support and precise lifting and lowering movement of the sample holding seat and the magnetic mechanism, meeting the requirements of different experimental needs or processing stages.

[0015] Preferably, the magnetic mechanism comprises a heat-conducting plate body and a magnet, and the magnet is arranged on the upper surface of the heat-conducting plate body.

[0016] In this technical solution, the magnet is installed on the upper surface of the heat-conducting plate body and is tightly combined with the heat-conducting plate body. The position of the magnet on the upper surface of the heat-conducting plate body can be adjusted and fixed as needed. Through the arrangement of the magnet, the magnetic mechanism can generate a magnetic field on the heat-conducting plate body, thereby achieving the attraction and manipulation of the magnetic beads in the sample solution.

[0017] Preferably, the heat-conducting plate is a copper plate, and the heat-conducting property of the copper plate helps to maintain the stable temperature of the magnetic mechanism and the sample solution, and improve the thermal balance of the device.

[0018] Preferably, the refrigeration mechanism is installed on the lower surface of the heat conducting plate body.

[0019] In this technical solution, by installing the refrigeration mechanism on the lower surface of the heat-conducting plate body, the sample placement device can achieve rapid and uniform cooling of the sample solution. The thermal conductivity of the heat-conducting plate body helps to conduct the cooling effect generated by the refrigeration mechanism, so that the sample holding seat can reach the required low temperature in a shorter time.

[0020] Preferably, the refrigeration mechanism is a semiconductor refrigerator, and the refrigeration surface of the semiconductor refrigerator is in contact with the lower surface of the heat conduction plate body.

[0021] In the technical solution, by adopting a semiconductor refrigerator and fitting its cooling surface with the lower surface of the heat-conducting plate body, the sample placement device can achieve efficient and accurate sample cooling.

[0022] Preferably, the sample placement device further comprises a radiator, and the radiator is arranged on one side of the heating surface of the semiconductor refrigerator.

[0023] In this technical solution, the heat sink is installed on one side of the heating surface of the semiconductor refrigerator, which can effectively transfer the heat generated during the heating process to the heat sink and accelerate the heat dissipation.

[0024] Preferably, the heat sink is a fin structure, and the heat sink is in contact with the heating surface of the semiconductor refrigerator.

[0025] In this technical solution, the fin structure of the heat sink can increase the surface area for heat dissipation, improve the heat dissipation efficiency, and accelerate the conduction of heat from the semiconductor refrigerator to the surrounding environment.

[0026] Preferably, the sample placement device further comprises a fan, wherein the fan is arranged on a side of the heat sink away from the semiconductor refrigerator, and an air outlet side of the fan is opposite to the fins of the heat sink.

[0027] In this technical solution, by providing a fan and installing it on the side of the radiator away from the semiconductor refrigerator, the sample placement device can further improve the heat dissipation effect. The airflow generated by the fan accelerates the exchange of heat with the surrounding air, helps to quickly take away the heat, and provides new cold air to the radiator, improving the overall heat dissipation efficiency. Such a design can enhance the heat dissipation capacity of the device, maintain the stability of the sample placement device, and ensure temperature control and stability during sample processing.

[0028] Preferably, the sample placement device further comprises a heat insulating member, the heat insulating member is provided with a notch, the semiconductor refrigerator is arranged in the notch, and the outer circumference of the semiconductor refrigerator is in contact with the inner circumference of the notch.

[0029] In this technical solution, a heat insulating member is provided in the sample placement device to isolate the heat of the semiconductor refrigerator. The outer peripheral side of the semiconductor refrigerator fits the inner peripheral side of the notch to form a close contact to reduce heat leakage. The presence of the heat insulating member can effectively isolate the heat conduction of the refrigerator, prevent heat from being transferred to other parts, and improve the cooling effect and energy utilization.

[0030] Preferably, the fixing frame is provided with a limiting member, the limiting member has a strip hole extending along the axial direction of the screw rod, and the supporting member is provided with a limiting column, the limiting column passes through the strip hole and can move along the extension direction of the strip hole.

[0031] In this technical solution, a limiter is provided on the fixing frame of the sample placement device to limit the moving range of the support. The moving range of the support can be limited by the cooperation between the limiter column and the strip hole to ensure that it moves within the specified range of movement.

[0032] The utility model also provides an extractor, comprising the sample placing device as described above.

[0033] The positive and progressive effects of the utility model are as follows: the design of the sample holding seat enables the sample solution to be conveniently placed on the stage, providing good sample fixing and supporting functions. The refrigeration mechanism is located below the sample holding seat, which can refrigerate the sample to ensure that the sample is processed or stored at an appropriate temperature. The setting of the magnetic mechanism enables the device to attract the magnetic beads in the sample solution, which is convenient for separation from the sample liquid. When it is necessary to adsorb the magnetic beads in the sample, the magnetic mechanism can move up and down relative to the sample holding seat. This adsorption operation can conveniently achieve the separation, aggregation or other processing requirements of the magnetic beads. When it is not necessary to adsorb the magnetic beads, the magnetic mechanism can be moved to a position away from the sample holding seat, so that the magnetic beads are no longer affected by the magnetic force, thereby stopping adsorption. Such operational flexibility makes it possible to control the adsorption state of the magnetic beads according to the needs of the experimental process or processing steps, thereby improving the freedom and controllability of the operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is an overall structural diagram of a sample placement device according to a preferred embodiment of the utility model.

[0035] Figure 2 It is a schematic diagram of a sample holding seat according to a preferred embodiment of the utility model.

[0036] Figure 3 It is a schematic diagram of a refrigeration module according to a preferred embodiment of the present invention.

[0037] Figure 4 This is an exploded view of a refrigeration module according to a preferred embodiment of the present invention.

[0038] Figure 5 Schematic diagram of a loading platform according to a preferred embodiment of the present invention.

[0039] Description of reference numerals:

[0040] Sample placement device 1

[0041] Stage 11

[0042] Retaining edge 111

[0043] Sample holder 12

[0044] Insulation box 13

[0045] Insulation box cover 14

[0046] Semiconductor Refrigerator 21

[0047] Heat conducting plate 31

[0048] Magnet 32

[0049] Support member 41

[0050] Guide column 42

[0051] Sleeve bearing 421

[0052] Motor 43

[0053] Motor column 431

[0054] Nut 432

[0055] Driven wheel 433

[0056] Motor fixing plate 434

[0057] Drive wheel 435

[0058] Photoelectric switch 436

[0059] Screw 44

[0060] Screw sleeve part 45

[0061] Upper frame 46

[0062] Lower frame 47

[0063] Sliding seat 48

[0064] Radiator 5

[0065] Fan 6

[0066] Thermal insulation 7

[0067] Limiting piece 81

[0068] Limit column 82 DETAILED DESCRIPTION

[0069] The present invention will be described more clearly and completely below through preferred embodiments in conjunction with the accompanying drawings.

[0070] This embodiment provides a sample placement device 1, such as Figure 1 and 2 As shown, it includes a stage 11, a sample holding seat 12, a refrigeration mechanism, a heat preservation box body 13, and a heat preservation box cover 14. The sample holding seat 12 is arranged on the stage 11, and the sample holding seat 12 is provided with a plurality of liquid holding cavities, and the top of each liquid holding cavity has an opening, which is used to place the sample solution. The refrigeration mechanism is arranged below the sample holding seat 12. The sample holding seat 12 is a rectangular container, and a heat preservation box body 13 is arranged around the sample holding seat 12 on the stage 11, and the heat preservation box cover 14 is covered on the top of the heat preservation box body 13. The heat preservation box body 13 and the heat preservation box cover 14 together constitute a heat preservation box for keeping the sample solution warm.

[0071] like Figure 3As shown, a magnetic mechanism is provided between the refrigeration mechanism and the sample holding seat 12 , and the magnetic mechanism is configured to be able to move up and down relative to the sample holding seat 12 , and the magnetic mechanism is configured to be able to attract magnetic beads in the sample solution.

[0072] In this embodiment, if Figure 5 As shown, four rectangular hollows are provided on the stage 11, and the inner wall of the rectangular hollows is provided with a rib 111, and the sample receiving seat 12 is placed on the rib 111. The sample receiving seat 12 can be placed in any rectangular hollow according to the actual process step requirements.

[0073] The present embodiment enables the device to attract the nanomagnetic beads in the sample solution through the arrangement of the magnetic mechanism, so as to be conveniently separated from the sample liquid. When the nanomagnetic beads in the sample need to be adsorbed, the magnetic mechanism can move up and down relative to the sample holder 12, i.e., move to the bottom of the sample holder 12, and fit on the bottom surface of the sample holder 12, and this adsorption operation can easily realize the separation, aggregation or other processing requirements of the nanomagnetic beads. And when the nanomagnetic beads do not need to be adsorbed, the magnetic mechanism can move to a position away from the sample holder 12, i.e., move downward, so that the nanomagnetic beads are no longer affected by the magnetic force, thereby stopping adsorption. Such operational flexibility makes it possible to control the adsorption state of the nanomagnetic beads according to the needs of the experimental process or the processing steps, thereby improving the degree of freedom and controllability of the operation.

[0074] Figure 3 and Figure 4 The refrigeration module of this embodiment includes a refrigeration mechanism, a lifting mechanism, and a magnetic mechanism. The lifting mechanism includes a fixed frame and a support member 41. The fixed frame is provided with a guide column 42. The support member 41 and the guide column 42 are slidably matched. The magnetic mechanism is arranged on the support member 41. The lifting mechanism also includes a motor 43 and a screw 44. The screw 44 and the output shaft of the motor 43 are connected by a transmission belt (not shown in the figure). The support member 41 is provided with a screw sleeve part 45 threadedly connected to the screw 44. A nut 432 is provided in the screw sleeve part. The screw 44 and the nut 432 are threadedly connected. The axes of the screw 44 and the guide column 42 are parallel and extend along the moving direction of the magnetic mechanism. The fixing frame includes an upper frame 46 and a lower frame 47, and the upper frame 46 and the lower frame 47 are fixedly connected by a plurality of guide pillars 42. The support member 41 is provided with a sleeve seat 48 that slides with the plurality of guide pillars 42 in a one-to-one correspondence, wherein the outer wall of the guide pillar 42 is sleeved with a sleeve bearing 421, and the sleeve seat 48 is sleeved outside the sleeve bearing 421. The motor 43 of this embodiment can adopt a standard hybrid stepping motor with a model of MS11HS5P41505.0.

[0075] In this embodiment, the upper frame 46 and the lower frame 47 are rectangular frames, and one long side of the lower frame 47 has a rectangular motor mounting platform extending outward in a direction perpendicular to the long side, and four motor columns 431 are arranged on the motor mounting platform, and the top of the motor column 431 is fixedly connected to the motor fixing plate 434. A driving wheel 435 is arranged on the output shaft of the motor 43, and a driven wheel 433 is arranged on the screw 44. The motor 43 drives the driving wheel 435, and the driven wheel 433 is driven by the conveyor belt to rotate, thereby driving the screw 44 to rotate, so that the power of the motor 43 can be transmitted to the screw 44, so that the support member 41 and the magnetic mechanism can be lifted and lowered. Among them, the guide column 42 and the sliding sleeve seat 48 play a guiding role in the lifting process.

[0076] In this embodiment, the conveyor belt is a belt. In other embodiments, the transmission connection method may use a toothed belt or the like.

[0077] The magnetic mechanism includes a heat-conducting plate body 31 and a magnet 32, the magnet 32 ​​is arranged on the upper surface of the heat-conducting plate body 31, and the refrigeration mechanism is installed on the lower surface of the heat-conducting plate body 31. In the present embodiment, the magnet 32 ​​is arranged in a one-to-one correspondence with the liquid holding cavity of the sample holding seat 12. It should be noted that the present embodiment is only an example, and the position of the magnet 32 ​​on the upper surface of the heat-conducting plate body 31 can be adjusted and fixed as needed. By setting the magnet 32, the magnetic mechanism can generate a magnetic field on the heat-conducting plate body 31, thereby achieving the attraction and manipulation of the magnetic beads in the sample solution. The heat-conducting plate body 31 of the present embodiment is a copper plate, and the thermal conductivity of the copper plate helps to maintain the stable temperature of the magnetic mechanism and the sample solution and improve the thermal balance of the device. By installing the refrigeration mechanism on the lower surface of the heat-conducting plate body 31, the sample placement device 1 can achieve rapid and uniform cooling of the sample solution. In other embodiments, the heat-conducting plate body 31 can be made of other materials with good thermal conductivity, such as aluminum.

[0078] In this embodiment, the refrigeration mechanism is a semiconductor refrigerator 21, and there are two semiconductor refrigerators 21, which are placed in parallel and adjacent to each other, and the cooling surface of the semiconductor refrigerator 21 is in contact with the lower surface of the heat conducting plate 31. The sample placement device 1 also includes a heat sink 5, which is arranged on one side of the heating surface of the semiconductor refrigerator 21, and the heat sink 5 is a fin structure and is in contact with the heating surface of the semiconductor refrigerator 21. Specifically, the model of the semiconductor refrigerator 21 used in this embodiment is TEC2-1971222.

[0079] In other embodiments, the refrigerator may be a semiconductor refrigerator 21 of other types, or other types of refrigerators, such as a compressor refrigerator, a magnetic refrigerator, etc.

[0080] By using the semiconductor refrigerator 21 and fitting its cooling surface to the lower surface of the heat conducting plate 31, the sample placement device 1 can achieve efficient and accurate sample cooling. Installing the radiator 5 on the heating surface side of the semiconductor refrigerator 21 can effectively transfer the heat generated during the heating process to the radiator 5 and accelerate the heat dissipation. In other embodiments, the radiator 5 can also be other structures, such as a liquid cooling radiator, a pipe radiator, etc.

[0081] The sample placement device 1 further includes a fan 6 , which is disposed on a side of the heat sink 5 away from the semiconductor refrigerator 21 , and an air outlet side of the fan 6 is opposite to the fins of the heat sink 5 .

[0082] In this embodiment, the sample placement device 1 can further improve the heat dissipation effect by providing a fan 6 and installing it on the side of the radiator 5 away from the semiconductor refrigerator 21. The airflow generated by the fan 6 accelerates the exchange of heat with the surrounding air, helps to quickly remove the heat, and provides new cold air for the radiator 5, thereby improving the overall heat dissipation efficiency.

[0083] It should be noted that, in other embodiments, the heat dissipation and temperature reduction of the heating surface of the refrigeration mechanism may also be performed only by the fan 6 .

[0084] The sample placement device 1 further includes a heat insulating member 7, which is provided with a notch, and the semiconductor refrigerator 21 is arranged in the notch, and the outer peripheral side of the semiconductor refrigerator 21 is in contact with the inner peripheral side of the notch. Specifically, the heat insulating member 7 is a heat insulating cotton. In other embodiments, the heat insulating member 7 can be other heat insulating materials, such as foam plastics, ceramics with heat insulating properties, graphene, etc.

[0085] In this embodiment, the heat insulating member 7 is used to isolate the heat of the semiconductor refrigerator 21. The outer peripheral side of the semiconductor refrigerator 21 is fitted with the inner peripheral side of the notch to form a close contact to reduce heat leakage. The existence of the heat insulating member 7 can effectively isolate the heat conduction of the refrigerator, prevent the heat from being transferred to other parts, and improve the cooling effect and energy utilization.

[0086] In this embodiment, a stopper 81 is provided on the fixing frame, and the stopper 81 has a strip hole extending along the axial direction of the screw rod 44. A stopper column 82 is provided on the support member 41, and the stopper column 82 passes through the strip hole and can move along the extension direction of the strip hole. A photoelectric switch 436 is provided at the lower end and the clamp of the stopper 81, and the photoelectric switch 436 is electrically connected to the motor 43 to control whether the motor 43 starts working. The photoelectric switch 436 can use a slot-type photoelectric switch with a model of EE_SX954_R.

[0087] In this embodiment, a limiter 81 is provided on the fixing frame of the sample placement device 1 to limit the moving range of the support member 41. The limiter column 82 cooperates with the strip hole to ensure that the support member 41 moves within the specified range of movement. The limiter 81 in this embodiment is a plate-like structure for easy installation.

[0088] This embodiment also provides an extraction instrument, which includes the sample placement device 1 as described above.

[0089] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model is described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A sample placement device, characterized in that: The sample holding seat comprises an object stage, a sample holding seat, and a refrigeration mechanism. The sample holding seat is arranged on the object stage, the sample holding seat is used to place a sample solution, and the refrigeration mechanism is arranged below the sample holding seat. A magnetic mechanism is provided between the refrigeration mechanism and the sample holding seat. The magnetic mechanism is configured to be able to move up and down relative to the sample holding seat, and the magnetic mechanism is configured to be able to attract magnetic beads in the sample solution.

2. The sample placement device according to claim 1, characterized in that: The sample placement device further comprises a lifting mechanism, the lifting mechanism comprises a fixing frame and a supporting member, the fixing frame is provided with a guide column, the supporting member is slidably matched with the guide column, and the magnetic mechanism is arranged on the supporting member; The lifting mechanism further comprises a motor and a screw, wherein the motor is drivingly connected to the screw, and the support member is provided with a screw sleeve portion threadably connected to the screw; The axes of the screw rod and the guide column are parallel and extend along the moving direction of the magnetic mechanism.

3. The sample placement device according to claim 2, characterized in that: The screw rod is connected to the output shaft of the motor via a transmission belt.

4. The sample placement device according to claim 2, characterized in that: The fixing frame comprises an upper frame and a lower frame, wherein the upper frame and the lower frame are fixedly connected via a plurality of guide pillars, and a sliding sleeve seat which slides in cooperation with the plurality of guide pillars in a one-to-one correspondence is provided on the supporting member.

5. The sample placement device according to claim 1, characterized in that: The magnetic mechanism comprises a heat-conducting plate body and a magnet, and the magnet is arranged on the upper surface of the heat-conducting plate body.

6. The sample placement device according to claim 5, characterized in that: The heat conducting plate body is a copper plate.

7. The sample placement device according to claim 5, characterized in that: The refrigeration mechanism is installed on the lower surface of the heat conducting plate body.

8. The sample placement device according to claim 5, characterized in that: The refrigeration mechanism is a semiconductor refrigerator, and the refrigeration surface of the semiconductor refrigerator is in contact with the lower surface of the heat conduction plate body.

9. The sample placement device according to claim 8, characterized in that: The sample placement device also includes a radiator, which is arranged on one side of the heating surface of the semiconductor refrigerator.

10. The sample placement device according to claim 9, characterized in that: The heat sink is a fin structure, and the heat sink is in contact with the heating surface of the semiconductor refrigerator.

11. The sample placement device according to claim 10, characterized in that: The sample placement device further comprises a fan, which is arranged on a side of the radiator away from the semiconductor refrigerator, and an air outlet side of the fan is opposite to the fins of the radiator.

12. The sample placement device according to claim 8, characterized in that: The sample placement device also includes a heat insulating member, which is provided with a notch, the semiconductor refrigerator is arranged in the notch, and the outer circumference of the semiconductor refrigerator is in contact with the inner circumference of the notch.

13. The sample placement device according to claim 2, characterized in that: The fixing frame is provided with a limiting piece, the limiting piece has a strip hole extending along the axial direction of the screw rod, and the supporting piece is provided with a limiting column, the limiting column passes through the strip hole and can move along the extending direction of the strip hole.

14. An extraction apparatus, characterized in that: Comprising a sample placement device as described in any one of claims 1-13.

Images