Novel preparation and extraction device for NK immune cells

Abstract

The utility model relates to the field of NK immune cell preparation, and discloses a novel NK immune cell preparation and extraction device which comprises a centrifugal frame, a rectangular groove is formed in the centrifugal frame, electromagnets are fixedly installed on the inner walls of the two sides of the rectangular groove, and two clamping blocks are arranged in the rectangular groove. The electromagnets are arranged on the two sides of the clamping blocks, repulsion magnets are installed on the sides, deviating from each other, of the two clamping blocks respectively, the sides, corresponding to the electromagnets on the two sides, of the two repulsion magnets are homopolar, an NK immune cell centrifugal tank is arranged between the two clamping blocks, when centrifugation is needed, peripheral blood cells are injected into the NK immune cell centrifugal tank, then the input ends of the electromagnets are connected with a power source, and the electromagnets are connected with the power source. The electromagnets on the two sides are controlled to be opened and closed by external power supply control equipment, and the electromagnets on the two sides are intermittently started to repel the clamping blocks corresponding to the electromagnets so as to perform reciprocating motion for centrifugation.

Description

Technical Field

[0001] The utility model relates to the field of NK immune cell preparation, in particular to a novel NK immune cell preparation and extraction device. Background Art

[0002] Natural killer cells are important immune cells in the body. They are not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in the occurrence of hypersensitivity reactions and autoimmune diseases in certain cases. They can recognize target cells and killing mediators. Natural killer cells are derived from bone marrow lymphoid stem cells. Their differentiation and development depend on the bone marrow and thymus microenvironment. They are mainly distributed in the bone marrow, peripheral blood, liver, spleen, lungs and lymph nodes.

[0003] The existing NK immune cells generally need to be separated by centrifugation filtration during preparation and extraction, such as the existing equipment CN210506320U, a NK separation and extraction device based on stable centrifugation technology, including a box body and an upper cover movably arranged at the upper port of the box body; a rotating block is rotatably arranged at a position near the upper port inside the box body, and the rotating block is rotatably connected to the inner wall of the box body through a bearing ring, and a plurality of mounting blocks are distributed in an array on the upper end of the rotating block, and a plurality of test tubes to be separated are arranged on the mounting block, and a rotating shaft is arranged in the middle position of the lower end of the mounting block, and a fixed bearing is arranged between the rotating shaft and the rotating block. Rotating connection, a rotating gear is provided at the lower end of the rotating shaft. The utility model improves on the shortcomings of the existing device, so that the test tube has both orbital and rotational characteristics, which helps to improve the centrifugal effect. In addition, the transmission ratio between the driven pulley group and the driving pulley can be adjusted, which makes it convenient for the staff to adjust the centrifugal speed in a targeted manner, and it is highly practical. However, the above-mentioned equipment only uses rotary centrifugation during centrifugation. Therefore, during centrifugation, a large amount of supernatant will be generated on the liquid surface, and it needs to be filtered and collected with a filter later, which easily causes incomplete separation. For this reason, we propose a new NK immune cell preparation and extraction device. Utility Model Content

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the utility model provides a novel NK immune cell preparation and extraction device to solve the above-mentioned problems.

[0006] (II) Technical solution

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a novel NK immune cell preparation and extraction device, comprising a centrifugal frame, a rectangular groove is provided on the centrifugal frame, and electromagnets are fixedly installed on the inner walls of both sides of the rectangular groove, and two clamping blocks are arranged inside the rectangular groove, and repulsive magnets are respectively installed on the sides of the two clamping blocks that are away from each other, and the two repulsive magnets and the sides corresponding to the electromagnets on both sides are magnets with the same poles, and a NK immune cell centrifugal tank is arranged between the two clamping blocks. When centrifugation is required, peripheral blood cells are injected into the NK immune cell centrifugal tank, and then the input end of the electromagnet is connected to a power supply, and the opening and closing of the electromagnets on both sides are controlled by an external power supply control device. The electromagnets on both sides are started intermittently, and when one side of the electromagnet is started, it will repel the corresponding clamping block, so that the clamping block slides to the other side. When it slides to the position of the electromagnet on the other side, the electromagnet on the other side is started, and the corresponding clamping block is repelled, and reciprocating motion is performed to centrifuge. The centrifugation of this scheme corresponds to the existing rotary centrifugation method, and more NK immune cells can be collected on the NK immune cell centrifugal filter, which is easy to use and has a better separation effect.

[0008] Preferably, two guide rods are fixedly installed inside the rectangular groove, and a sliding block is fixedly installed at a position of the clamping block corresponding to the guide rod, and the sliding block is movably sleeved with the guide rod.

[0009] Preferably, a screw rod is fixedly installed on the two sliders on one side, and the end of the screw rod passes through the slider on the other side. The end of the screw rod is threadedly connected to a nut, and the nut is rotated to make the nut displace laterally on the screw rod, so that the two clamps clamp the NK immune cell centrifuge tank between the two clamps.

[0010] Preferably, a shaking filter structure is provided inside the NK immune cell centrifuge tank.

[0011] Preferably, the shaking filter structure includes a filter connecting frame, which is annular and fits the inner wall of the NK immune cell centrifugal tank. A plurality of groups of NK immune cell centrifugal filters are equidistantly arranged inside the filter connecting frame.

[0012] Preferably, an inner wall groove is opened on the inner wall of the filter connecting frame, and a rectangular block is fixedly installed at the position of the NK immune cell centrifugal filter corresponding to the inner wall groove, and the rectangular block is adapted to the inner wall groove, and the rectangular block and the inner wall groove are slidably engaged together. When the NK immune cell centrifugal tank is shaken left and right, the filter connecting frame will follow the shaking inside the NK immune cell centrifugal tank due to inertia. At this time, the NK immune cell centrifugal filter inside the filter connecting frame will follow the movement, thereby performing reciprocating filtration separation, so that the separation effect is better.

[0013] (III) Beneficial effects

[0014] Compared with the prior art, the utility model provides a novel NK immune cell preparation and extraction device, which has the following beneficial effects:

[0015] 1. In the novel NK immune cell preparation and extraction device, when the NK immune cell centrifugal tank is shaken left and right, the filter connection frame will follow the shaking due to inertia inside the NK immune cell centrifugal tank. At this time, the NK immune cell centrifugal filter inside the filter connection frame will follow the movement, thereby performing reciprocating filtration and separation, so that the separation effect is better.

[0016] 2. When centrifugation is required, the novel NK immune cell preparation and extraction device injects peripheral blood cells into the NK immune cell centrifuge tank, then connects the input end of the electromagnet to a power source, and uses an external power control device to control the opening and closing of the electromagnets on both sides. The electromagnets on both sides are started intermittently, and when one side of the electromagnet is started, it repels the corresponding clamping block, causing the clamping block to slide to the other side. When it slides to the position of the electromagnet on the other side, the electromagnet on the other side is started, repels the corresponding clamping block, and performs reciprocating motion back and forth for centrifugation. The centrifugation of this scheme corresponds to the existing rotary centrifugation method, and can collect more NK immune cells on the NK immune cell centrifugal filter, is easy to use, and has a better separation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional schematic diagram of the utility model;

[0018] Figure 2 This is a schematic cross-sectional view of the NK immune cell centrifugation tank.

[0019] In the figure: 1, centrifugal frame; 2, electromagnet; 3, rectangular groove; 4, guide rod; 5, slider; 6, clamping block; 7, screw rod; 8, nut; 9, NK immune cell centrifugal tank; 10, filter connecting frame; 11, NK immune cell centrifugal filter; 12, inner wall groove; 13, rectangular block; 14, repelling magnet. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See also Figure 1-2A novel NK immune cell preparation and extraction device comprises a centrifugal frame 1, a rectangular groove 3 is provided on the centrifugal frame 1, an electromagnet 2 is fixedly installed on the inner walls of both sides of the rectangular groove 3, two clamping blocks 6 are arranged inside the rectangular groove 3, and repulsive magnets 14 are respectively installed on the sides of the two clamping blocks 6 that are away from each other, and the two repulsive magnets 14 are magnets with the same poles as the electromagnets 2 on both sides, and a NK immune cell centrifugal tank 9 is arranged between the two clamping blocks 6. When centrifugation is required, peripheral blood cells are injected into the NK immune cell centrifugal tank 9, and then the input end of the electromagnet 2 is connected to The power supply uses an external power supply control device to control the opening and closing of the electromagnets 2 on both sides. The electromagnets 2 on both sides are started intermittently. When the electromagnet 2 on one side is started, it will repel the corresponding clamp 6, causing the clamp 6 to slide to the other side. When it slides to the position of the electromagnet 2 on the other side, the electromagnet 2 on the other side is started at this time, and repels the corresponding clamp 6, performing reciprocating motion back and forth for centrifugation. The centrifugation of this scheme corresponds to the existing rotary centrifugation method, and can collect more NK immune cells on the NK immune cell centrifugal filter 11, is easy to use, and has a better separation effect.

[0022] Furthermore, two guide rods 4 are fixedly installed inside the rectangular groove 3 , and a sliding block 5 is fixedly installed at the position of the clamping block 6 corresponding to the guide rod 4 , and the sliding block 5 is movably sleeved with the guide rod 4 .

[0023] Furthermore, a screw rod 7 is fixedly installed on the two sliders 5 on one side, and the end of the screw rod 7 passes through the slider 5 on the other side. The end of the screw rod 7 is threadedly connected with a nut 8. By rotating the nut 8, the nut 8 is laterally displaced on the screw rod 7, so that the two clamps 6 clamp the NK immune cell centrifuge tank 9 between the two clamps 6.

[0024] Furthermore, a shaking filter structure is arranged inside the NK immune cell centrifugal tank 9 .

[0025] Furthermore, the shaking filter structure includes a filter connecting frame 10, which is annular and fits the inner wall of the NK immune cell centrifugal tank 9. A plurality of groups of NK immune cell centrifugal filters 11 are equidistantly arranged inside the filter connecting frame 10.

[0026] Furthermore, an inner wall groove 12 is opened on the inner wall of the filter connecting frame 10, and a rectangular block 13 is fixedly installed at the position of the NK immune cell centrifugal filter 11 corresponding to the inner wall groove 12, and the rectangular block 13 is adapted to the inner wall groove 12, and the rectangular block 13 and the inner wall groove 12 are slidably engaged together. When the NK immune cell centrifugal tank 9 is shaken left and right, the filter connecting frame 10 will follow the shaking due to inertia inside the NK immune cell centrifugal tank 9. At this time, the NK immune cell centrifugal filter 11 inside the filter connecting frame 10 will follow the movement, thereby performing reciprocating filtration separation, so that the separation effect is better.

[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A novel NK immune cell preparation and extraction device, comprising a centrifugal rack (1), It is characterized in that The centrifugal frame (1) is provided with a rectangular groove (3), and electromagnets (2) are fixedly mounted on the inner walls of both sides of the rectangular groove (3). Two clamping blocks (6) are arranged inside the rectangular groove (3), and repulsive magnets (14) are respectively mounted on the sides of the two clamping blocks (6) facing away from each other, and the two repulsive magnets (14) have the same pole as the corresponding sides of the electromagnets (2) on both sides, and an NK immune cell centrifugal tank (9) is arranged between the two clamping blocks (6).

2. A novel NK immune cell preparation and extraction device according to claim 1, Features: Two guide rods (4) are fixedly installed inside the rectangular groove (3), and a sliding block (5) is fixedly installed at a position of the clamping block (6) corresponding to the guide rod (4), and the sliding block (5) is movably sleeved with the guide rod (4).

3. A novel NK immune cell preparation and extraction device according to claim 2, Features: A screw rod (7) is fixedly mounted on the two sliders (5) on one side, the end of the screw rod (7) passes through the slider (5) on the other side, and a nut (8) is threadedly connected to the end of the screw rod (7).

4. A novel NK immune cell preparation and extraction device according to claim 1, Features: The NK immune cell centrifugal tank (9) is provided with a shaking filter structure inside.

5. A novel NK immune cell preparation and extraction device according to claim 4, Features: The shaking filter structure comprises a filter connection frame (10), the filter connection frame (10) is annular, and the filter connection frame (10) is in close contact with the inner wall of the NK immune cell centrifugal tank (9), and a plurality of groups of NK immune cell centrifugal filters (11) are equidistantly arranged inside the filter connection frame (10).

6. A novel NK immune cell preparation and extraction device according to claim 5, Features: An inner wall groove (12) is provided on the inner wall of the filter connecting frame (10); a rectangular block (13) is fixedly installed at a position of the NK immune cell centrifugal filter (11) corresponding to the inner wall groove (12); the rectangular block (13) is adapted to the inner wall groove (12), and the rectangular block (13) and the inner wall groove (12) are slidably engaged with each other.

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