Porous calibration plate
By designing porous calibration plates and spherical samples, the problem of single use of existing calibration sheets is solved, and convenient verification of performance parameters of a variety of biomedical instruments is achieved. It is suitable for different microscopic analysis equipment and prevents cross-contamination.
Patent Information
- Application Number
- CN202420911850.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-28
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-04-28
AI Technical Summary
The existing calibration sheets have a single purpose and cannot easily verify multiple performance parameters of biomedical instruments, especially for inverted microscopes and high-content systems, and can only verify a single performance parameter at a time.
A porous calibration plate was designed, including multi-porous plates and sphere samples. By incorporating different types of sphere samples in the storage space of the porous plates, such as monodispersed fluorescent microspheres, size standard microspheres, counting standard microspheres, etc., and Through the removable plate structure and limiting projection groove design, it is easy to adapt to different equipment and prevent cross-contamination.
It realizes convenient verification of the performance parameters of a variety of biomedical instruments, is suitable for different microscopic analysis equipment, prevents cross-contamination, and simplifies the verification process of multiple performance parameters.
Smart Images
Figure CN222240328U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biomedical instrument calibration, in particular to a multi-hole calibration plate. Background Art
[0002] When testing and using biomedical instruments, it is usually necessary to calibrate the biomedical instruments to determine their performance. For example, tissue and cell staining is used to determine the performance of the instrument under bright field and fluorescence channels, and immunofluorescence staining of tissues and cells is used to determine the performance parameters and calibration of the instrument under the fluorescence channel. For example, when counting cell viability, calculating particle size, and counting, it is necessary to measure and analyze the relevant cell viability, particle size standards, and technical standards.
[0003] Currently, biomedical instruments are calibrated using calibration sheets. However, calibration sheets are single-purpose and only applicable to upright microscopes, making them inconvenient for calibrating inverted microscopes and high-content systems. Calibration sheets can only calibrate a single performance parameter at a time, and cannot calibrate multiple performance parameters; when calibrating multiple performance parameters, a different calibration sheet must be replaced for each performance parameter. Utility Model Content
[0004] Based on the above description, the utility model provides a multi-hole calibration plate, aiming to solve the problems of single use and inconvenient calibration of existing calibration plates.
[0005] The technical solution of the utility model to solve the above technical problems is as follows:
[0006] A multi-hole calibration plate, comprising:
[0007] Perforated plates;
[0008] The spherical sample is arranged in the accommodation space of the porous plate;
[0009] The protective film is disposed on the spherical sample to cover the spherical sample.
[0010] On the basis of the above technical solution, the present invention can also be improved as follows.
[0011] Furthermore, the spherical sample is a monodisperse fluorescent microsphere, a size standard microsphere, a counting standard microsphere or a special standard microsphere.
[0012] Furthermore, the monodisperse fluorescent microspheres include polystyrene microspheres with a diameter of 0.05 to 20 μm and surface functional groups thereof.
[0013] Furthermore, the size-standard microspheres include polystyrene microspheres with a diameter of 0.02 to 200 μm.
[0014] Furthermore, the counting standard microspheres include polystyrene microspheres with a diameter of 0.5 to 20 μm and trace amounts of surfactants.
[0015] Furthermore, the special standard microspheres include monodisperse polystyrene microspheres with a diameter of 5 μm or 7 μm.
[0016] Furthermore, the distribution density of the monodisperse fluorescent microspheres is 500 to 5000 per mm 2 The distribution density of the size standard microspheres is 5 to 5000 / mm 2 The distribution density of the counting standard microspheres and the special standard microspheres is 50 to 5000 / mm 2 .
[0017] Furthermore, the porous plate includes a first plate body and a second plate body arranged in sequence along the vertical direction, and the first plate body has a plurality of through holes; when the first plate body and the second plate body are assembled, the through holes and the second plate body together form a complete accommodating space.
[0018] Furthermore, each through hole has a first groove extending axially at one end close to the second plate body, and the second plate body is provided with a baffle corresponding to the first groove; when the first plate body and the second plate body are assembled, the baffle is inserted into the first groove.
[0019] Furthermore, the first plate body is provided with a frame extending toward the second plate body, at least one pair of inner walls of the frame is provided with a limiting group consisting of at least two limiting protrusions, the second plate body is provided with a second groove extending away from the first plate body, and the second groove is provided with a limiting groove corresponding to each limiting protrusion; when the first plate body and the second plate body are assembled, the frame is inserted into the second groove, and the limiting protrusion is inserted into the limiting groove.
[0020] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:
[0021] (1) The present application uses a porous plate and spherical samples. According to usage requirements, one type of spherical sample is placed in a certain storage space of the porous plate, or different types of spherical samples are placed in different storage spaces of the porous plate, so as to adapt to microscopic analysis equipment for different analysis purposes.
[0022] (2) The present application enables the first plate body and the second plate body to be detachably connected, thereby facilitating the cleaning of the through hole and the second plate body and preventing cross-contamination of different ball samples.
[0023] (3) The present application not only prevents the first plate body from being separated from the second plate body, but also facilitates the disassembly and assembly of the first plate body and the second plate body by cooperating with the limiting protrusion and the limiting groove. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 This is a general assembly diagram of a multi-hole calibration plate provided in an embodiment of the utility model;
[0025] Figure 2 An exploded view of a multi-hole calibration plate provided in an embodiment of the utility model;
[0026] Figure 3 It is a structural schematic diagram of the first plate body from another perspective in the embodiment of the utility model;
[0027] Figure 4 for Figure 3 A partial enlarged view of the middle part;
[0028] Figure 5 This is a schematic diagram of the structure of the second plate body in the embodiment of the utility model;
[0029] Figure 6 for Figure 5 A partial enlarged view of point B in the middle.
[0030] In the accompanying drawings, the components represented by the reference numerals are listed as follows:
[0031] 10. Perforated plate; 101. Accommodating space; 11. First plate body; 111. First groove; 112. Frame; 1121. Position-limiting protrusion; 12. Second plate body; 121. Baffle; 122. Second groove; 1221. Position-limiting groove; 20. Spherical sample; 30. Protective film. DETAILED DESCRIPTION
[0032] In order to facilitate understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. Embodiments of the present application are provided in the drawings. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.
[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.
[0034] It will be appreciated that spatial relationship terms such as "under," "beneath," "below," "under," "above," "above," etc., may be used herein to describe the relationship of an element or feature shown in the figures to other elements or features. It will be appreciated that, in addition to the orientations shown in the figures, spatial relationship terms also include different orientations of the device in use and operation. For example, if the device in the accompanying drawings is flipped, an element or feature described as "under other elements" or "under it" or "under it" will be oriented as being "above" the other elements or features. Thus, the exemplary terms "under" and "under" may include both upper and lower orientations. In addition, the device may also include additional orientations (e.g., rotated 90 degrees or other orientations), and the spatial descriptors used herein are interpreted accordingly.
[0035] When used herein, the singular forms "a", "an", and "said / the" may also include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "include / comprise" or "have" etc. specify the presence of stated features, wholes, steps, operations, components, parts or combinations thereof, but do not exclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts or combinations thereof.
[0036] See attached Figures 1 to 6 As shown, the utility model provides a technical solution: a porous calibration plate, comprising a porous plate 10, a spherical sample 20 and a protective film 30, wherein the spherical sample 20 is arranged in a receiving space 101 of the porous plate 10; the protective film 30 is arranged on the spherical sample 20 to cover the spherical sample 20.
[0037] Exemplarily, the number of holes in the porous plate 10 is six holes, twelve holes, or twenty-four holes, etc. There are one or more types of spherical sample 20, and the type of spherical sample 20 is selected according to the performance parameter to be verified. For example, when the performance parameter to be verified is one, a corresponding type of spherical sample 20 is selected according to the performance parameter, and the spherical sample 20 is placed in a certain accommodation space 101 of the porous plate 10; when the performance parameter to be verified is multiple, a corresponding plurality of spherical samples 20 are selected according to the performance parameters, and the plurality of spherical samples 20 are placed one by one in a plurality of accommodation spaces 101 of the porous plate 10 (i.e., different types of spherical samples 20 are placed in different accommodation spaces 101).
[0038] According to this embodiment, the multi-well plate 10 and the spherical sample 20 can be used to meet various requirements, thereby providing convenience for microscope verification.
[0039] Optionally, the spherical sample 20 is a monodisperse fluorescent microsphere, a size standard microsphere, a counting standard microsphere or a special standard microsphere.
[0040] For example, the monodisperse fluorescent microspheres include polystyrene microspheres with a diameter of 0.05 to 20 μm and surface functional groups thereof.
[0041] For example, size standard microspheres include polystyrene microspheres having a diameter of 0.02 to 200 μm.
[0042] For example, counting standard microspheres include polystyrene microspheres with a diameter of 0.5 to 20 μm and trace amounts of surfactants.
[0043] For example, special standard microspheres include monodisperse polystyrene microspheres having a diameter of 5 μm or 7 μm.
[0044] For example, the distribution density of monodisperse fluorescent microspheres is 500 to 5000 / mm 2 The distribution density of standard size microspheres is 5 to 5000 / mm 2 The distribution density of counting standard microspheres and special standard microspheres is 50 to 5000 / mm 2 .
[0045] For example, the protective film 30 is solidified by an anti-fluorescence quenching agent, neutral gum and glycerol gelatin.
[0046] See attached Figures 1 to 3 As shown in Figure 5, in some embodiments, the porous plate 10 includes a first plate body 11 and a second plate body 12 arranged in sequence along the vertical direction, and the first plate body 11 has a plurality of through holes; when the first plate body 11 and the second plate body 12 are assembled, the through holes and the second plate body 12 together form a complete accommodating space 101.
[0047] Exemplarily, the first plate body 11 and the second plate body 12 may be connected by fasteners; for example, the fasteners are screws or bolts.
[0048] According to this embodiment, by detachably connecting the first plate body 11 and the second plate body 12 , it is possible to facilitate cleaning of the through hole and the second plate body 12 , thereby preventing cross contamination of different ball samples 20 .
[0049] See attached Figures 1 to 3 As shown in Figure 5, in some embodiments, each through hole has a first groove 111 extending axially at one end close to the second plate body 12, and the second plate body 12 is provided with a baffle 121 corresponding to the first groove 111; when the first plate body 11 and the second plate body 12 are assembled, the baffle 121 is inserted into the first groove 111.
[0050] According to this embodiment, the baffle 121 cooperates with the first groove 111 , which not only facilitates the cleaning of the through hole and the second plate body 12 , but also prevents the liquid of the spherical sample 20 from flowing out, thereby preventing cross-contamination of different spherical samples 20 .
[0051] See attached Figures 2 to 6 As shown, in some embodiments, the first plate body 11 is provided with a frame 112 extending toward the second plate body 12, at least one pair of inner walls of the frame 112 are provided with a limiting group consisting of at least two limiting protrusions 1121, and the second plate body 12 is provided with a second groove 122 extending away from the first plate body 11, and the second groove 122 is provided with a limiting groove 1221 corresponding to each limiting protrusion 1121; when the first plate body 11 and the second plate body 12 are assembled, the frame 112 is inserted into the second groove 122, and the limiting protrusion 1121 is inserted into the limiting groove 1221.
[0052] According to this embodiment, the cooperation between the limiting protrusion 1121 and the limiting groove 1221 can not only prevent the first plate body 11 from being separated from the second plate body 12 , but also facilitate the disassembly and assembly of the first plate body 11 and the second plate body 12 .
[0053] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A porous calibration plate, characterized in that: include: A porous plate (10); A spherical sample (20) is arranged in the containing space (101) of the porous plate (10); A protective film (30) is disposed on the spherical sample (20) to cover the spherical sample (20).
2. A multi-hole calibration plate according to claim 1, characterized in that: The spherical sample (20) is a monodisperse fluorescent microsphere, a size standard microsphere, a counting standard microsphere or a special standard microsphere.
3. A multi-hole calibration plate according to claim 2, characterized in that: The monodisperse fluorescent microspheres include polystyrene microspheres with a diameter of 0.05 to 20 μm and surface functional groups thereof.
4. A multi-hole calibration plate according to claim 2, characterized in that: The size standard microspheres include polystyrene microspheres with a diameter of 0.02 to 200 μm.
5. A multi-hole calibration plate according to claim 2, characterized in that: The counting standard microspheres include polystyrene microspheres with a diameter of 0.5 to 20 μm and trace surfactants thereof.
6. A multi-hole calibration plate according to claim 2, characterized in that: The special standard microspheres include monodisperse polystyrene microspheres with a diameter of 5 μm or 7 μm.
7. A multi-hole calibration plate according to claim 2, characterized in that: The distribution density of the monodisperse fluorescent microspheres is 500 to 5000 per mm 2 The distribution density of the size standard microspheres is 5 to 5000 / mm 2 The distribution density of the counting standard microspheres and the special standard microspheres is 50 to 5000 / mm 2 .
8. A multi-hole calibration plate according to any one of claims 1 to 7, characterized in that: The porous plate (10) comprises a first plate body (11) and a second plate body (12) which are arranged in sequence along a vertical direction, wherein the first plate body (11) has a plurality of through holes; when the first plate body (11) and the second plate body (12) are assembled, the through holes and the second plate body (12) together form a complete accommodating space (101).
9. A multi-hole calibration plate according to claim 8, characterized in that: Each through hole has a first groove (111) extending in the axial direction at one end close to the second plate body (12); the second plate body (12) is provided with a baffle (121) corresponding to the first groove (111); when the first plate body (11) and the second plate body (12) are assembled, the baffle (121) is inserted into the first groove (111).
10. A multi-hole calibration plate according to claim 9, characterized in that: The first plate body (11) is provided with a frame (112) extending toward the second plate body (12); at least one pair of inner walls of the frame (112) is provided with a limiting group consisting of at least two limiting protrusions (1121); the second plate body (12) is provided with a second groove (122) extending away from the first plate body (11); the second groove (122) is provided with a limiting groove (1221) corresponding to each limiting protrusion (1121); when the first plate body (11) and the second plate body (12) are assembled, the frame (112) is inserted into the second groove (122), and the limiting protrusion (1121) is inserted into the limiting groove (1221).