Silica gel chromatographic column capable of rapidly measuring chromatography sample loading amount
The silica gel chromatography column addresses the lack of direct measurement by integrating a flow switch control body and engraved scales, facilitating direct reading of required amounts, thus improving the convenience and efficiency of silica gel column chromatography operations.
Patent Information
- Application Number
- CN202420805944.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-18
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-04-18
AI Technical Summary
Silica gel column chromatography lacks direct measurement of sample loading amount and silica gel powder mass, making operations inconvenient and requiring pre-calculation, which complicates the use of different column types.
A silica gel chromatography column with integrated features such as a flow switch control body, plug valve, frosted port, and engraved scales for sample loading and silica gel powder mass, allowing direct reading of required amounts.
Enables convenient and quick operation by directly reading the required silica gel powder mass and sample loading amount, enhancing the practicality and efficiency of silica gel column chromatography.
Smart Images
Figure CN222259268U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of column chromatography, in particular to a silica gel chromatographic column which can quickly measure the chromatographic sample loading amount. Background Art
[0002] Silica gel column chromatography is a chromatographic technology that uses the difference in functional groups of different substances to separate them, resulting in differences in their adsorption capacity with silica gel. Generally, substances with more polar functional groups are strongly adsorbed by silica gel, while substances with fewer polar functional groups are poorly adsorbed by silica gel, resulting in differences in the elution time during the chromatography process. The entire chromatography process is a process of adsorption, desorption, re-adsorption, and re-desorption.
[0003] Silica gel column chromatography usually uses an open glass column, and the ratio of column length to diameter is generally around 25:1. As the column length increases, the movement path of the separated components becomes longer, and the separation effect becomes better. There is no scale marking on the silica gel chromatography column currently used. The quality of the silica gel used is mainly determined by the mass-density-volume relationship formula (m=ρv) and the cylinder volume formula (V=πr 2 *h), the sample loading amount used is mainly calculated by the appropriate ratio of sample loading amount to silica gel mass (1:30~1:50). During use, the quality of silica gel powder and the appropriate sample loading amount cannot be directly grasped, which brings some inconvenience to the use of silica gel column chromatography. In order to solve the above technical problems, a silica gel chromatographic column that can quickly measure the chromatographic sample loading amount is proposed. Summary of the invention
[0004] The utility model aims to provide a silica gel chromatography column which can quickly measure the chromatographic sample loading amount, can directly read the appropriate sample loading amount and the mass of the silica gel powder used, so that the silica gel column chromatography operation is more convenient and quick, and solves the problem in the prior art that it is impossible to directly understand the sample loading amount of different column types and the mass of the silica gel powder used, and in the process of silica gel column chromatography, the mass of the silica gel used needs to be calculated in advance, making the silica gel column chromatography inconvenient to use.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a silica gel chromatographic column capable of quickly measuring the chromatographic sample loading, comprising a column, a flow switch control body is integrally formed at the bottom end of the column, a plug valve is installed inside the flow switch control body, the plug valve is fixed to one side of the flow switch control body by a nut, and a sealing ring is provided between the plug valve and the nut; a flow guide hole in the middle of the plug valve faces the discharge port of the column, and the right end of the plug valve passes through the inside of the flow switch control body and is screwed to the left end of the plug valve; a mark showing the silica gel model and the inner diameter of the chromatographic column is engraved on the upper end of the column; a frosted port is integrally formed at the upper end of the column, the frosted port is arranged in an inverted trapezoidal shape, a frosted layer is provided on the inner end surface of the frosted port, and the frosted layer can be connected to a liquid storage device with a frosted layer on the outer end surface or directly connected to a pressure pump; a sand plate is provided at the lower end of the column to prevent silica gel from blocking the flow switch control body; a sample loading scale is engraved on the front of the column, and a silica gel powder mass scale is engraved on the back.
[0006] Preferably, the length of the column is 200-300 mm, the inner diameter is 10-100 mm, and the ratio of the length to the diameter of the column (1) is 2:1-30:1.
[0007] Preferably, the stopcock is a component made of polytetrafluoroethylene.
[0008] Preferably, the frosted port adopts a 24 / 29 standard caliber.
[0009] Preferably, the sand plate is a component made of high borosilicate glass.
[0010] Preferably, the marks of the sample loading volume scale and the silica gel powder mass scale are both formed by carving, and the marks are either convex or concave.
[0011] Preferably, the calibration of the sample loading scale and the silica gel powder mass scale is performed based on the volume and mass of each type of silica gel at room temperature and a standard atmospheric pressure.
[0012] Preferably, the chromatographic column can be used alone or in combination with a liquid storage device.
[0013] Preferably, the capacity of the liquid storage device is 100-1000 mL.
[0014] Compared with the prior art, the beneficial effects of the utility model are as follows:
[0015] The silica gel chromatographic column can quickly measure the chromatographic sample loading amount. The calibration of the sample loading amount scale and the silica gel powder mass scale are calibrated according to the volume and mass of various types of silica gel at room temperature and a standard atmospheric pressure. The scale is carved on the column tube in a convex or concave manner. When in use, the mass of the required silica gel powder and the appropriate sample loading amount of the crude product to be separated under the silica gel mass condition can be directly read according to the scale; the operator can also directly add silica gel powder of appropriate mass to the column according to the mass of the crude product to be separated. The column is highly practical and greatly increases the convenience of operation. It is also convenient to use with a detachable liquid storage device, thereby improving the convenience of operating the chromatographic column for silica gel column chromatography. The chromatographic sample loading amount and the silica gel powder mass are used together to make the selection and operation of the chromatographic column for silica gel column chromatography more convenient. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model, wherein the left side figure is a front view and the right side figure is a back view.
[0017] Figure 2 This is a schematic diagram of the column structure of the utility model, wherein the left side figure is a front view and the right side figure is a back view.
[0018] Figure 3 It is a schematic diagram of the rotary valve plug structure of the utility model.
[0019] In the figure: 1. Column; 2. Flow switch control tube; 3. Stopcock; 4. Nut; 5. Sealing ring; 6. Frosted mouth; 7. Sand plate; 8. Liquid storage device; 9. Sample loading volume scale; 10. Silica gel mass scale; 11. Silica gel model and chromatography column inner diameter identification. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0021] See also Figures 1 to 3A silica gel chromatography column capable of rapidly measuring the amount of sample loaded in a chromatography, comprising a column body 1, wherein the length of the column body 1 is 200-300 mm, the inner diameter is 10-100 mm mm, the column length to diameter ratio of the column (1) is 2:1~30:1, the bottom end of the column 1 is integrally formed with a flow switch control body 2, the flow switch control body 2 is internally installed with a plug valve 3, the plug valve 3 is a polytetrafluoroethylene material component, the plug valve 3 is fixed to one side of the flow switch control body by a nut 4, and a sealing ring 5 is provided between the plug valve 3 and the nut 4; the guide hole in the middle of the plug valve 3 is directly opposite to the discharge port of the column 1, the right end of the plug valve 3 passes through the inside of the flow switch control body 2 and is screwed to the left end of the plug valve 3; the upper end of the column 1 is engraved with a mark 11 showing the silica gel model and the inner diameter of the chromatography column; the upper end of the column 1 is integrally formed with a frosted port 6, the frosted port 6 adopts a 24 / 29 standard caliber, the frosted port 6 is arranged in an inverted trapezoidal shape, the inner end surface of the frosted port 6 is provided with a frosted layer, and can be connected to a liquid storage device 8 with a frosted layer on the outer end surface or directly connected to a pressure pump, the capacity of the liquid storage device is 100~1000 mL; a sand plate 7 is arranged at the lower end of the column 1, and the sand plate 7 is a component made of high borosilicate glass, which can prevent silica gel from clogging the flow switch control body 2; a sample loading scale 9 is engraved on the front side of the column, and a silica gel powder mass scale 10 is engraved on the back side. The marks of the sample loading scale 9 and the silica gel powder mass scale 10 are both formed by engraving, and the marks are either convex or concave. The calibration of the sample loading scale 9 and the silica gel powder mass scale 10 is based on the volume and mass of various types of silica gel at room temperature and a standard atmospheric pressure.
[0022] The silica gel chromatographic column capable of rapidly measuring the chromatographic sample loading amount has a sample loading amount scale 9 and a silica gel powder mass scale 10 added to a column body 1. When in use, the mass of the required silica gel powder and the appropriate sample loading amount of the crude product to be separated under the silica gel mass condition can be directly read according to the scale; the operator can also directly add silica gel powder of appropriate mass into the column according to the mass of the crude product to be separated, which is highly practical, greatly increases the convenience of operation, and is convenient to use with a detachable liquid storage device, thereby improving the convenience of operating the chromatographic column for silica gel column chromatography; the chromatographic sample loading amount and the silica gel powder mass are used together, so that the selection and operation of the chromatographic column for silica gel column chromatography are more convenient, and the efficiency of rapidly selecting various types of chromatographic columns is improved.
[0023] In summary: the silica gel chromatography column which can quickly measure the chromatography loading amount is calibrated according to the volume and mass of various types of silica gel under room temperature and a standard atmospheric pressure as the standard for calibrating the loading amount scale 9 and the silica gel powder mass scale 10. The loading amount scale 9 and the silica gel powder mass scale 10 are carved on the column body 1 in a convex or concave manner. When in use, the loading amount and the mass of the silica gel powder used can be directly read according to the scale, which increases the convenience of operation, improves the efficiency of chromatography work, has strong practicality, and makes silica gel column chromatography operation more convenient and quick.
[0024] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A silica gel chromatography column capable of rapidly measuring the chromatographic loading amount, comprising a column (1), characterized in that: The bottom end of the column (1) is integrally formed with a flow switch control body (2), and a plug valve (3) is installed inside the flow switch control body (2); the plug valve (3) is fixed to one side of the flow switch control body (2) through a nut (4), and a sealing ring (5) is provided between the plug valve (3) and the nut (4); the guide hole in the middle of the plug valve (3) faces the discharge port of the column (1), and the right end of the plug valve (3) passes through the inside of the flow switch control body (2) and is screwed to the left end of the plug valve (3); the upper end of the column (1) is engraved with a display silicone seal. The column (1) is provided with a frosted opening (6) integrally formed at the upper end thereof, the frosted opening (6) being arranged in an inverted trapezoidal shape, the inner end surface of the frosted opening (6) being provided with a frosted layer, and being connectable to a liquid storage device (8) having a frosted layer at the outer end surface thereof, or being directly connected to a pressure pump; the lower end of the column (1) is provided with a sand plate (7) to prevent silica gel from clogging the flow switch control body (2); the front side of the column (1) is engraved with a sample loading scale (9), and the back side is engraved with a silica gel powder mass scale (10).
2. A silica gel chromatography column capable of quickly measuring chromatography loading amount according to claim 1, characterized in that: The length of the column (1) is 200-300 mm, the inner diameter is 10-100 mm, and the ratio of the length to the diameter of the column (1) is 2:1-30:
1.
3. A silica gel chromatography column capable of quickly measuring chromatography loading amount according to claim 1, characterized in that: The plug valve (3) is a component made of polytetrafluoroethylene.
4. The silica gel chromatography column capable of quickly measuring the chromatography loading amount according to claim 1, characterized in that: The frosted port (6) adopts a 24 / 29 standard caliber.
5. The silica gel chromatography column capable of quickly measuring the chromatography loading amount according to claim 1, characterized in that: The sand plate (7) is a component made of high borosilicate glass.
6. The silica gel chromatography column capable of quickly measuring the chromatography loading amount according to claim 1, characterized in that: The markings of the sample loading volume scale (9) and the silica gel powder mass scale (10) are both formed by engraving, and the markings are either convex or concave.
7. The silica gel chromatography column capable of quickly measuring the chromatography loading amount according to claim 1, characterized in that: The calibration of the sample loading scale (9) and the silica gel powder mass scale (10) is carried out based on the volume and mass of each type of silica gel at room temperature and a standard atmospheric pressure.
8. The silica gel chromatography column capable of quickly measuring the chromatography loading amount according to claim 1, characterized in that: The chromatographic column can be used alone or in combination with the liquid storage device (8).
9. The silica gel chromatography column capable of quickly measuring the chromatography loading amount according to claim 1, characterized in that: The capacity of the liquid storage device (8) is 100-1000 mL.