Automatic online detection and feeding automatic feedback control system in microbial fermentation process

Abstract

The utility model relates to the technical field of microbial fermentation control, and provides an automatic online detection and automatic feedback control system for a microbial fermentation process, which is characterized in that a ceramic membrane is arranged in a filter cavity, the filter cavity is divided into an inner cavity and an outer cavity by the ceramic membrane, and the inner cavity is connected with a fermentation tank through a material discharge pipeline and a thallus return pipeline respectively; the outer cavity is connected with the dilution mixing pool through the clear liquid discharging pipeline, the dilution water supply pipeline is connected with the dilution mixing pool, and the dilution mixing pool is connected with the sampling pool; the material supplementing pipeline is connected with the fermentation tank, and the control mainboard is configured to control the sampling assembly to act so as to suck feed liquid from the sampling pool, move the feed liquid to the detection assembly for detection and control the material supplementing pipeline to supplement materials to the fermentation tank according to a detection result of the detection assembly. Therefore, fermentation liquor in the fermentation tank cannot be polluted while sampling detection and material supplementation can be completed on line, the whole fermentation process is free of bacterial contamination risk, and the whole process is easy and convenient to operate and saves time and labor.

Description

Technical Field

[0001] The utility model relates to the technical field of microbial fermentation control, in particular to an automatic online detection and material supplementation automatic feedback control system for a microbial fermentation process. Background Art

[0002] With the development of bioengineering, biofermentation technology has also been widely developed in the fields of agriculture, industry, medicine, pharmacology, energy, environmental protection, chemical raw materials, etc. During the microbial fermentation process, the fermentation broth needs to be tested to ensure that no bacteria are produced in the microbial fermentation process and the required microorganisms or metabolites are obtained. During sampling, the culture medium sample is manually taken out from the culture tank. Before sampling, high-pressure steam is passed through the sampling port for sterilization for 30 minutes, and then the sampling port is opened to release the front section sample, and the middle and rear section samples are taken for rapid packaging. After the sampling is completed, high-pressure steam needs to be passed through the sampling port for sterilization for 30 minutes. The removed sample is moved to the centrifuge for centrifugal separation, and the supernatant is taken to the analytical equipment for detection and analysis. Generally, the centrifugal equipment and analytical equipment are located in other laboratories, and there are inevitable intervals in space and time. After the operator obtains the sample test results, he determines whether to feed according to the calculation. If feeding is required, the feeding equipment is manually controlled for quantitative feeding. The above-mentioned existing technology has the following technical defects: First, the sterilization operation before and after sampling is cumbersome and time-consuming, and cannot meet the high sampling frequency demand; second, the entire detection process includes manual sampling, centrifugation, detection, and feeding, which takes a long time, and the content of the components of the detected items in the culture tank is constantly changing. When the amount of feeding is calculated, the content of the detected items in the culture tank may have changed, so the feeding cannot achieve the expected effect; third, the manual sampling amount cannot be accurately controlled, and multiple sampling and monitoring cannot be achieved for the cell culture industry with less culture medium; fourth, the entire existing detection process requires manual participation in operation, which consumes a lot of labor costs.

[0003] Microbial fermentation refers to the process of using microorganisms to convert raw materials into products needed by humans through specific metabolic pathways under suitable conditions. In recent years, with the continuous progress and development of biotechnology, biofermentation engineering technology and related products are affecting people's daily lives to a greater extent. In the process of biological fermentation, multi-stage continuous fermentation systems have higher fermentation production efficiency, so multi-stage fermentation systems are usually used for fermentation operations. During the fermentation process, it is necessary to continuously feed the fermenter through a feeding tank to maintain the continuous fermentation work. Utility Model Content

[0004] The utility model provides an automatic online detection and feeding automatic feedback control system for a microbial fermentation process, which is used to solve the problem of complicated manual sampling and feeding steps in the prior art.

[0005] The utility model provides an automatic online detection and feeding automatic feedback control system for microbial fermentation process.

[0006] Including: fermentation tank, filter chamber, control main board, sampling component, feed pipeline, detection component, dilution mixing tank, sampling tank and dilution water supply pipeline;

[0007] A ceramic membrane is provided in the filter cavity, and the ceramic membrane divides the filter cavity into an inner cavity and an outer cavity, the inner cavity is connected to the fermentation tank through a material discharge pipeline and a bacterial cell reflux pipeline respectively, the outer cavity is connected to the dilution mixing tank through a clear liquid discharge pipeline, the dilution water supply pipeline is connected to the dilution mixing tank, and the dilution mixing tank is connected to the sampling tank;

[0008] The feed pipeline is connected to the fermentation tank, and the sampling component, the feed pipeline and the detection component are all electrically connected to the control main board;

[0009] The control main board is configured to control the action of the sampling component to draw feed liquid from the sampling pool, move the feed liquid to the detection component for detection, and control the feed pipeline to feed the fermentation tank according to the detection result of the detection component.

[0010] According to the utility model, an automatic online detection and feeding automatic feedback control system for a microbial fermentation process also includes a steam supply pipeline, which is connected to the bacteria return pipeline through a pneumatic three-way diaphragm valve, and the bacteria return pipeline is connected to the fermentation tank through a first sterile sampling valve, the first sterile sampling valve is also connected to a sampling return flow drainage pipeline, and a first temperature sensor, a first pneumatic diaphragm valve and a first manual diaphragm valve are sequentially arranged on the sampling return flow drainage pipeline, the material discharge pipeline is connected to the fermentation tank through a second sterile sampling valve, the second sterile sampling valve is also connected to a sampling discharge drainage pipeline, and a second temperature sensor, a second pneumatic diaphragm valve and a second manual diaphragm valve are sequentially arranged on the sampling discharge drainage pipeline.

[0011] According to an automatic online detection and feeding automatic feedback control system for a microbial fermentation process provided by the utility model, it also includes a cleaning liquid supply pipeline, which is connected to the steam supply pipeline through a first pneumatic three-way ball valve, and the cleaning liquid supply pipeline is also connected to the outer cavity through a pneumatic ball valve, and a second pneumatic three-way ball valve is arranged on the clear liquid discharge pipeline.

[0012] According to an automatic online detection and feeding automatic feedback control system for a microbial fermentation process provided by the utility model, a third pneumatic three-way ball valve is arranged on the pipeline connecting the pneumatic ball valve to the outer cavity, and the third pneumatic three-way ball valve is connected to the outer cavity exhaust pipeline.

[0013] According to the utility model, an automatic online detection and feeding automatic feedback control system for a microbial fermentation process is provided, which also includes a parameter detector, which is arranged in the clear liquid discharge pipeline, and is used to detect at least one of the sugar content and the transmittance, and a second pump body is arranged on the dilution water supply pipeline.

[0014] According to an automatic online detection and feeding automatic feedback control system for a microbial fermentation process provided by the utility model, the sampling pool is connected to a waste liquid collection pool through a liquid outlet pipeline.

[0015] According to an automatic online detection and feeding automatic feedback control system for a microbial fermentation process provided by the utility model, the sampling component includes a moving mechanism and a sampling needle, the sampling needle is connected to the moving mechanism, and the moving mechanism is electrically connected to the control main board. Under the control of the control main board, the moving mechanism drives the sampling needle to absorb the feed liquid from the sampling pool and move the feed liquid to the detection component for detection.

[0016] According to an automatic online detection and automatic feeding feedback control system for a microbial fermentation process provided by the utility model, the feeding pipeline includes a first feeding pipeline and / or a second feeding pipeline, the first feeding pipeline is sequentially provided with a third pneumatic diaphragm valve, a first flowmeter, a first pneumatic regulating valve and a fourth pneumatic diaphragm valve, the second feeding pipeline is sequentially provided with a fifth pneumatic diaphragm valve, a second flowmeter, a second pneumatic regulating valve and a sixth pneumatic diaphragm valve, the first flowmeter, the first pneumatic regulating valve, the second flowmeter and the second pneumatic regulating valve are all electrically connected to the control mainboard;

[0017] The control main board is configured to control the action of the first pneumatic regulating valve and / or the second pneumatic regulating valve according to the detection result of the detection component, so that the flow value of the first flow meter and / or the flow value of the second flow meter are equal to the corresponding target flow value.

[0018] The utility model provides an automatic online detection and automatic feeding feedback control system for a microbial fermentation process. By arranging a ceramic membrane in the filter chamber, the bacteria in the fermentation liquid can flow back into the fermentation tank, and the clear liquid of the fermentation liquid can flow to the dilution mixing tank. The diluted clear liquid can be subjected to corresponding detection and analysis in the sampling tank. In this way, under the control of the control main board, the fermentation tank can realize real-time automatic separation of bacteria, automatic sampling and detection, and automatically adjust the feeding rate or frequency according to the detection results and the preset program to realize automatic control of the feeding of the fermentation process. In this way, continuous sampling can be realized, the sampling density can be subdivided into minutes, and there is no risk of contamination in the entire sampling process. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction will be given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 It is a structural schematic diagram of an automatic online detection and feeding automatic feedback control system for a microbial fermentation process provided by the utility model.

[0021] Reference numerals:

[0022] 1. Fermentation tank;

[0023] 2. Material discharge pipeline; 21. Second aseptic sampling valve; 22. Second pneumatic diaphragm valve; 23. Second manual diaphragm valve; 24. Second temperature sensor; 25. First pump body;

[0024] 3. Ceramic membrane; 31. Inner cavity; 32. Outer cavity;

[0025] 4. Bacteria return pipeline; 41. Pneumatic three-way diaphragm valve; 42. Pressure sensor; 43. Pneumatic diaphragm regulating valve; 44. First sterile sampling valve; 45. First pneumatic diaphragm valve; 46. First manual diaphragm valve; 47. First temperature sensor;

[0026] 5. Steam supply pipeline; 51. First pneumatic three-way ball valve;

[0027] 6. Cleaning liquid supply pipeline; 61. Third pneumatic three-way ball valve; 62. Pneumatic ball valve;

[0028] 7. External cavity drain pipeline;

[0029] 8. Clear liquid discharge pipeline; 81. Second pneumatic three-way ball valve; 82. Third pump body; 83. Parameter detector;

[0030] 9. External cavity sewage pipeline; 10. Dilution mixing tank;

[0031] 11. Dilution water supply pipeline; 111. Second pump body;

[0032] 12. Sampling pool; 121. Waste liquid collection pool;

[0033] 13. Sampling assembly; 131. Moving mechanism; 132. Sampling needle;

[0034] 14. Detection component; 141. Enzyme membrane electrode detector; 142. Liquid chromatography detector; 15. Control main board;

[0035] 16. First feed pipeline; 161. Third pneumatic diaphragm valve; 162. Seventh pneumatic diaphragm valve; 163. First flow meter; 164. First pneumatic regulating valve; 165. Fourth pneumatic diaphragm valve; 166. Eighth pneumatic diaphragm valve;

[0036] 17. The second feed pipeline; 171. The fifth pneumatic diaphragm valve; 172. The ninth pneumatic diaphragm valve; 173. The second flow meter; 174. The second pneumatic regulating valve; 175. The sixth pneumatic diaphragm valve; 176. The tenth pneumatic diaphragm valve. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described clearly and completely in conjunction with the drawings in the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0038] like Figure 1 As shown, the automatic online detection and feeding automatic feedback control system for the microbial fermentation process of the embodiment of the utility model includes: a fermentation tank 1, a filter chamber, a dilution mixing tank 10, a sampling tank 12 and a dilution water supply pipeline 11.

[0039] A ceramic membrane 3 is provided in the filter chamber, and the ceramic membrane 3 divides the filter chamber into an inner chamber 31 and an outer chamber 32. The pore size of the ceramic membrane 3 is 50-200 nm. The inner chamber 31 is connected to the fermentation tank 1 through the material discharge pipeline 2 and the bacterial cell return pipeline 4, respectively. That is, the inner chamber 31 can form a loop with the inside of the fermentation tank 1. The outer chamber 32 is connected to the dilution mixing tank 10 through the clear liquid discharge pipeline 8, the dilution water supply pipeline 11 is connected to the dilution mixing tank 10, and the dilution mixing tank 10 is connected to the sampling tank 12.

[0040] The ceramic membrane 3 is an asymmetric membrane made of inorganic ceramic materials through a special process. The wall of the ceramic membrane 3 is densely covered with micropores. Under pressure, the raw liquid flows inside the membrane tube or outside the membrane, small molecules pass through the membrane, and large molecules are retained by the membrane, thereby achieving the purpose of separation, concentration, purification and environmental protection. For example, a large cylindrical ceramic membrane 3 can be placed in the filter cavity.

[0041] Among them, after the fermentation liquid of the fermenter 1 is filtered through the filter cavity, the bacteria can remain in the inner cavity 31 and flow back to the fermenter 1, while the clear liquid enters the outer cavity 32 and flows into the dilution mixing tank 10. In addition, the dilution water supply pipeline 11 can provide dilution water to the dilution mixing tank 10 according to the preset dilution multiple, so that the dilution water is mixed with the clear liquid flowing into the dilution mixing tank 10, and the mixed liquid flows into the sampling tank 12 for subsequent sampling and detection to perform corresponding component analysis, and the fermenter 1 is supplemented according to the component analysis structure.

[0042] It should be noted that a stirring device may be provided inside the dilution mixing tank 10, and the stirring device may include a stirring rod provided inside the dilution mixing tank 10 and a magnetic stirrer provided outside the dilution mixing tank 10, wherein the stirring rod is a magnet, and the magnetic stirrer is used to generate a changing magnetic field to drive the stirring rod to rotate. Under the action of the stirring device, the permeate from the ceramic membrane 3 and the dilution water can be mixed uniformly.

[0043] By arranging the ceramic membrane 3 in the filter cavity, the bacteria in the fermentation liquid can flow back into the fermentation tank 1, and the clear liquid of the fermentation liquid can flow to the dilution mixing tank 10. The diluted clear liquid can be subjected to corresponding detection and analysis in the sampling tank 12. In other words, the sampling can be completed online without contaminating the fermentation liquid in the fermentation tank 1, thereby keeping the entire fermentation process free of the risk of contamination. In addition, the entire sampling process is easy to operate, which can save time and effort.

[0044] like Figure 1 As shown, the automatic online detection and automatic feeding feedback control system for the microbial fermentation process also includes: a control main board 15, a sampling component 13, a feeding pipeline and a detection component 14, the feeding pipeline is connected to the fermentation tank 1, and the sampling component 13, the feeding pipeline and the detection component 14 are all electrically connected to the control main board 15.

[0045] The control main board 15 is configured to control the operation of the sampling component 13 to absorb the feed liquid from the sampling pool 12 and move the feed liquid to the detection component 14 for detection, and control the feed pipeline to feed the fermentation tank 1 according to the detection result of the detection component 14.

[0046] In the embodiment of the utility model, under the control of the control main board 15, the fermentation tank 1 can realize real-time automatic separation of bacteria, automatic sampling and detection, and automatically adjust the feeding rate or frequency according to the detection results and the preset program to realize automatic control of the feeding of the fermentation process. In this way, continuous sampling can be achieved, the sampling density can be subdivided to the minute level, and there is no risk of contamination in the entire sampling process.

[0047] In some embodiments, Figure 1As shown, a pressure sensor 42 and a pneumatic diaphragm regulating valve 43 are sequentially arranged on the bacterial cell return pipeline 4 along the bacterial cell flow direction, and a first pump body 25 is arranged on the material discharge pipeline 2 .

[0048] Among them, the automatic online detection and feeding automatic feedback control system of the microbial fermentation process can be provided with a control main board 15, and the stirring device, the first pump body 25, the pressure sensor 42 and the pneumatic diaphragm regulating valve 43 are all electrically connected to the control main board 15.

[0049] Specifically, the first pump body 25 pumps the fermentation liquid in the fermentation tank 1 into the inner cavity 31 of the ceramic membrane 3, and controls the liquid pressure in the inner cavity 31 of the ceramic membrane 3 by controlling the opening of the pneumatic diaphragm regulating valve 43 and the pressure sensor 42, so that the liquid in the inner cavity 31 of the ceramic membrane 3 passes through the tube wall of the ceramic membrane 3 at a certain speed to reach the outer cavity 32 of the ceramic membrane 3, thereby achieving the purpose of bacterial recovery.

[0050] In some embodiments, Figure 1 As shown, the automatic online detection and feeding automatic feedback control system for microbial fermentation process also includes a steam supply pipeline 5, which is connected to the bacterial reflux pipeline 4 through a pneumatic three-way diaphragm valve 41, and the bacterial reflux pipeline 4 is connected to the fermentation tank 1 through a first sterile sampling valve, and the first sterile sampling valve 44 is also connected to a sampling reflux drainage pipeline, and the sampling reflux drainage pipeline is sequentially provided with a first temperature sensor 47, a first pneumatic diaphragm valve 45 and a first manual diaphragm valve 46, and the material discharge pipeline 2 is connected to the fermentation tank 1 through a second sterile sampling valve 21, and the second sterile sampling valve 21 is also connected to a sampling discharge drainage pipeline, and the sampling discharge drainage pipeline is sequentially provided with a second temperature sensor 24, a second pneumatic diaphragm valve 22 and a second manual diaphragm valve 23. Among them, the steam supply pipeline 5 is used to provide high-temperature steam.

[0051] It can be understood that the pneumatic three-way diaphragm valve 41, the first sterile sampling valve 44, the first temperature sensor 47, the first pneumatic diaphragm valve 45, the first manual diaphragm valve 46, the second sterile sampling valve 21, the second temperature sensor 24, the second pneumatic diaphragm valve 22 and the second manual diaphragm valve 23 can all be electrically connected to the control main board 15.

[0052] It should be noted that the pneumatic three-way diaphragm valve 41 is located between the pressure sensor 42 and the pneumatic diaphragm regulating valve 43 , and the first sterile sampling valve 44 is located on the downstream side of the pneumatic diaphragm regulating valve 43 , and the first pump body 25 is located between the second sterile sampling valve 21 and the outer cavity 32 .

[0053] In the embodiment of the utility model, when sterilizing the filter cavity, the first sterile sampling valve 44 and the second sterile sampling valve 21 are in a closed state, and the pneumatic three-way diaphragm valve 41, the pneumatic diaphragm regulating valve 43, the first pneumatic diaphragm valve 45, the first manual diaphragm valve 46, the second pneumatic diaphragm valve 22 and the second manual diaphragm valve 23 are all in an open state, so that external steam enters the ceramic membrane 3 and its affiliated pipelines for sterilization operation, and the steam size is controlled by the interlocking with the first temperature sensor 47 and the second temperature sensor 24, so that the ceramic membrane 3 and the affiliated pipelines reach a sterile state. After the set time is reached, the above valves are closed to complete the sterilization operation of the ceramic membrane 3 and the affiliated pipelines. Among them, the clear liquid discharge pipeline 8 can be connected to the external cavity sewage discharge pipeline 9.

[0054] It is particularly important to point out that when normal offline sampling is required, the first manual diaphragm valve 46 or the second manual diaphragm valve 23 is manually closed, and the second aseptic sampling valve 21 and the second pneumatic diaphragm valve 22, or the first aseptic sampling valve 44 and the first pneumatic diaphragm valve 45 are opened by controlling the main board 15, and then the first manual diaphragm valve 46 or the second manual diaphragm valve 23 is manually opened to complete the sampling operation.

[0055] In some embodiments, Figure 1 As shown, it also includes a cleaning liquid supply pipeline 6, which is connected to the steam supply pipeline 5 through a first pneumatic three-way ball valve 51, and the cleaning liquid supply pipeline 6 is also connected to the outer cavity 32 through a pneumatic ball valve 62, and a second pneumatic three-way ball valve 81 is provided on the clear liquid discharge pipeline 8. Among them, the first pneumatic three-way ball valve 51, the pneumatic ball valve 62 and the second pneumatic three-way ball valve 81 can all be electrically connected to the control motherboard 15. Among them, the cleaning water provided by the cleaning liquid supply pipeline 6 can be divided into sterile water and cleaning water with a certain antibacterial ability, such as dilute acid, dilute alkali, sodium hypochlorite, etc. It should be noted that the cleaning water with antibacterial ability is used for cleaning water when the ceramic membrane 3 is blocked and for protection water of the system when it is shut down.

[0056] It is understandable that after the entire fermentation operation is completed, a cleaning operation can be performed. Specifically, the first sterile sampling valve 44 and the second sterile sampling valve 21 are closed, the pneumatic three-way diaphragm valve 41 is opened, the first pneumatic three-way ball valve 51 is switched to the connecting cleaning liquid supply pipeline 6, and the first pump body 25 is reversed. In this way, the cleaning water is discharged from the sampling return sewage pipeline and the sampling discharge sewage pipeline to the external sewage pipe. The bacterial reflux pipeline 4 will be cleaned first, and the first pneumatic diaphragm valve 45 will be closed after cleaning. The first pump body 25 is in the forward direction, and the pressure of the inner cavity 31 of the ceramic membrane 3 is controlled by the pressure sensor 42. The pneumatic ball valve 62 is opened, and the outer cavity 32 of the ceramic membrane 3 to the sampling pool 12 and related pipelines can be cleaned. Among them, the second pneumatic three-way ball valve 81 is connected to the outer cavity sewage pipeline 9.

[0057] In some embodiments, Figure 1 As shown, a third pneumatic three-way ball valve 61 is provided on the pipeline connecting the pneumatic ball valve 62 to the outer chamber 32 .

[0058] It should be noted that the third pneumatic three-way ball valve 61 can be connected to the outer cavity exhaust pipe 7. In this way, after the outer cavity 32 of the ceramic membrane 3 to the sampling pool 12 and related pipelines are cleaned, high-temperature gas can be introduced through the outer cavity exhaust pipe 7 to enable the entire system to be quickly dried.

[0059] In some embodiments, Figure 1 As shown, the automatic online detection and feeding automatic feedback control system for microbial fermentation process also includes a parameter detector 83, which is arranged on the clear liquid discharge pipeline 8, and the parameter detector 83 is used to detect at least one of the sugar content value and the transmittance, and the dilution water supply pipeline 11 is provided with a second pump body 111. Among them, the parameter detector 83 is arranged between the second pneumatic three-way ball valve 81 and the dilution mixing tank 10, and the second pneumatic three-way ball valve 81 and the parameter detector 83 can also be provided with a third pump body 82. In this way, the parameter detector 83, the second pump body 111 and the third pump body 82 can all be electrically connected to the control mainboard 15.

[0060] It should be noted that the parameter detector 83 may include a first instrument for detecting the sugar content value and a second instrument for detecting the transmittance, or the parameter detector 83 may detect the sugar content value and the transmittance at the same time.

[0061] Exemplarily, the parameter detector 83 includes a detection cell and an incident light generator and a receiving light detector respectively located on opposite sides of the detection cell. The detection cell is made of quartz or glass. The parameter detector 83 is used to detect the absorbance value or transmittance value or refractive index of the liquid at different wavelengths.

[0062] It should be noted that when the inner cavity 31 of the ceramic membrane 3 is cleaned by the cleaning water provided by the cleaning liquid supply pipeline 6, when the parameter detector 83 detects that the transmittance of the permeated liquid is close to 100%, it can be determined that the cleaning of the inner cavity 31 of the ceramic membrane 3 has been completed.

[0063] It can be understood that the third pump body 82 pumps the permeate to the dilution mixing tank 10. According to the sugar content measured by the parameter detector 83 and the historical data stored in the control main board 15, the control main board 15 can determine the required dilution multiple. Under the control of the control main board 15, the second pump body 111 pumps the corresponding amount of dilution water into the dilution mixing tank 10.

[0064] As can be seen from the above, the absorbance at 600nm is monitored by the parameter detector 83. A sudden increase in the absorbance indicates that bacteria have penetrated into the outer cavity 32 at the ceramic membrane 3, which means that an alarm is issued to shut down for maintenance; by detecting the sugar content of the liquid, the sugar content has a certain corresponding relationship with the product content. According to the calculated product concentration and the detection concentration required by the control main board 15, the dilution multiple is calculated, and the corresponding dilution is automatically completed in the dilution mixing tank 10. In addition, for repeated production processes, the dilution multiples required for different fermentation cycles are relatively fixed. The control main board 15 can execute the system default dilution multiple. When the test result deviates greatly from the expected result, the dilution multiple of the next sample is re-determined according to the deviation, and an alarm is issued to prompt the main control personnel to investigate the cause.

[0065] In some embodiments, Figure 1 As shown, the sampling pool 12 is connected to a waste liquid collection pool 121 through a liquid outlet pipeline. It should be noted that the waste liquid collection pool 121 usually has sufficient capacity to accommodate all waste liquids flowing out of the sampling pool 12. The waste liquid collection pool 121 can ensure that the waste liquid flowing out of the sampling pool 12 will not pollute the environment. Exemplarily, the liquid that has passed through the dilution mixing pool 10 enters the bottom of the sampling pool 12, and then flows out from the top side of the sampling pool 12 to the waste liquid collection pool 121.

[0066] In some embodiments, Figure 1 As shown, the sampling component 13 includes a moving mechanism 131 and a sampling needle 132. The sampling needle 132 is connected to the moving mechanism 131. The moving mechanism 131 is electrically connected to the control motherboard 15. Under the control of the control motherboard 15, the moving mechanism 131 drives the sampling needle 132 to absorb the slurry from the sampling pool 12 and move the slurry to the detection component 14 for detection.

[0067] It should be noted that the moving mechanism 131 may be a two-dimensional moving mechanism 131 or a three-dimensional moving mechanism 131 , and both the two-dimensional moving mechanism 131 and the three-dimensional moving mechanism 131 may be a robot arm in the prior art, which is not specifically limited here.

[0068] Among them, the detection component 14 can include an enzyme membrane electrode detector 141 and a liquid chromatography detector 142, the enzyme membrane electrode detector 141 and the liquid chromatography detector 142 are placed on the experimental table, the moving mechanism 131 can move linearly in the horizontal direction and move up and down in the vertical direction, and a detachable rubber cover is provided on the top of the sampling pool 12. The sampling needle 132 can be inserted into the sampling pool 12 and absorb a quantitative sample liquid under the drive of the moving mechanism 131. After sampling, the sampling needle 132 accurately locates the enzyme membrane electrode detector 141 or the liquid chromatography detector 142 under the drive of the moving mechanism 131 to perform corresponding sampling detection.

[0069] In some embodiments, Figure 1As shown, the feeding pipeline includes the first feeding pipeline 16 and / or the second feeding pipeline 17 , that is, the feeding pipeline includes the first feeding pipeline 16 , or the feeding pipeline includes the second feeding pipeline 17 , or the feeding pipeline includes the first feeding pipeline 16 and the second feeding pipeline 17 .

[0070] The first feeding pipeline 16 is provided with a third pneumatic diaphragm valve 161, a first flow meter 163, a first pneumatic regulating valve 164 and a fourth pneumatic diaphragm valve 165 in sequence, and the second feeding pipeline 17 is provided with a fifth pneumatic diaphragm valve 171, a second flow meter 173, a second pneumatic regulating valve 174 and a sixth pneumatic diaphragm valve 175 in sequence. The first flow meter 163, the first pneumatic regulating valve 164, the second flow meter 173 and the second pneumatic regulating valve 174 are all electrically connected to the control main board 15.

[0071] The control motherboard 15 is configured to control the operation of the first pneumatic regulating valve 164 and / or the second pneumatic regulating valve 174 according to the detection result of the detection component 14, so that the flow value of the first flow meter 163 and / or the flow value of the second flow meter 173 are equal to the corresponding target flow value.

[0072] It should be noted that during the fermentation operation, the first sterile sampling valve 44 and the second sterile sampling valve 21 are opened, the first pneumatic diaphragm valve 45 and the second pneumatic diaphragm valve 22 are closed, and the first pump body 25 pumps the fermentation liquid in the fermenter 1 into the inner cavity 31 of the ceramic membrane 3, and controls the liquid pressure of the inner cavity 31 of the ceramic membrane 3 through the opening of the pneumatic diaphragm regulating valve 43 and the pressure sensor 42, so that the liquid in the inner cavity 31 of the ceramic membrane 3 passes through the tube wall of the ceramic membrane 3 at a certain speed to reach the outer cavity 32 of the ceramic membrane 3, thereby achieving the purpose of filtering and sterilization. At the same time, the third pump body 82 pumps the permeate to the dilution mixing tank 10, and determines the required dilution multiple according to the sugar content measured by the parameter detector 83 and the historical data stored in the control main board 15, and the corresponding amount of dilution water is pumped into the dilution mixing tank 10 by the second pump body 111, and flows to the sampling tank 12 after being stirred and mixed by the stirring device. The sampling needle 132 is driven by the moving mechanism 131 to regularly absorb the feed liquid from the sampling pool 12 according to the preset program, and transfers it to the enzyme membrane electrode detector 141 or the liquid chromatography detector 142 for detection. The detection result can be fed back to the control main board 15, and the control main board 15 calculates the flow of the first feed pipeline 16 and / or the second feed pipeline 17 according to the detection value and the preset conditions, and adjusts the opening of the first pneumatic regulating valve 164 and / or the second pneumatic regulating valve 174, so that the flow value of the first flow meter 163 and / or the second flow meter 173 reaches the new set value.

[0073] It should be noted that the outlet of the third pneumatic diaphragm valve 161 is connected to the seventh pneumatic diaphragm valve 162, and the inlet of the fourth pneumatic diaphragm valve 165 is connected to the eighth pneumatic diaphragm valve 166. The outlet of the fifth pneumatic diaphragm valve 171 is connected to the ninth pneumatic diaphragm valve 172, and the inlet of the sixth pneumatic diaphragm valve 175 is connected to the tenth pneumatic diaphragm valve 176. Among them, the seventh pneumatic diaphragm valve 162, the eighth pneumatic diaphragm valve 166, the ninth pneumatic diaphragm valve 172 and the tenth pneumatic diaphragm valve 176 are all electrically connected to the control mainboard 15.

[0074] In this way, when the fermentation tank is sterilized or sterilized, the third pneumatic diaphragm valve 161 is closed, the fourth pneumatic diaphragm valve 165, the seventh pneumatic diaphragm valve 162 and the eighth pneumatic diaphragm valve 166 are opened, so that the high-temperature steam in the fermentation tank is discharged from the system through the seventh pneumatic diaphragm valve 162 and the eighth pneumatic diaphragm valve 166, and the disinfection of the first feeding pipeline 16 between the third pneumatic diaphragm valve 161 and the fermentation tank 1 is completed. The second feeding pipeline is the same as the first feeding pipeline. The pipeline disinfection principle between the third pneumatic diaphragm valve 161 and the feeding source is the same. At this time, the fourth pneumatic diaphragm valve 165 is closed, and the third pneumatic diaphragm valve 161, the seventh pneumatic diaphragm valve 162 and the eighth pneumatic diaphragm valve 166 are opened. The disinfection method of the second feeding pipeline is the same as that of the first feeding pipeline. It should be particularly pointed out that the purpose of the automatic online detection of the microbial fermentation process and the automatic feedback control system for feeding is to be used for sampling and detection, so the pipeline and chamber volume of the entire system are very small. That is, the total volume of the outer cavity 32 of the ceramic membrane 3, the dilution mixing tank 10, the sampling tank 12 and the connecting pipeline is designed to be 10~20mL. Taking the total volume of 10mL as an example, when the sample liquid is not diluted, the permeation rate of the ceramic membrane 3 only needs to reach 10mL / min, and it can be ensured that the state of the sample liquid when sampling in the sampling tank 12 represents the fermentation liquid 1 minute ago. The detection time of the enzyme membrane electrode detector 141 is generally within 10min, and the detection time of the liquid chromatography detector 142 is generally within 30min. Since the fermentation process is a gradual process, delayed control within 1 hour is completely acceptable for experiments or production processes.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. An automatic online detection and feeding automatic feedback control system for microbial fermentation process, characterized in that: include: Fermentation tank, filter chamber, control main board, sampling assembly, feed pipeline, detection assembly, dilution mixing tank, sampling tank and dilution water supply pipeline; A ceramic membrane is provided in the filter cavity, and the ceramic membrane divides the filter cavity into an inner cavity and an outer cavity, the inner cavity is connected to the fermentation tank through a material discharge pipeline and a bacterial cell reflux pipeline respectively, the outer cavity is connected to the dilution mixing tank through a clear liquid discharge pipeline, the dilution water supply pipeline is connected to the dilution mixing tank, and the dilution mixing tank is connected to the sampling tank; The feed pipeline is connected to the fermentation tank, and the sampling component, the feed pipeline and the detection component are all electrically connected to the control main board; The control main board is configured to control the action of the sampling component to draw feed liquid from the sampling pool, move the feed liquid to the detection component for detection, and control the feed pipeline to feed the fermentation tank according to the detection result of the detection component.

2. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 1, characterized in that: A pressure sensor and a pneumatic diaphragm regulating valve are sequentially arranged on the bacterial cell return pipeline along the bacterial cell flow direction, and a first pump body is arranged on the material discharge pipeline.

3. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 1, characterized in that: It also includes a steam supply pipeline, which is connected to the bacteria return pipeline through a pneumatic three-way diaphragm valve, and the bacteria return pipeline is connected to the fermentation tank through a first sterile sampling valve, and the first sterile sampling valve is also connected to a sampling return sewage pipeline, and a first temperature sensor, a first pneumatic diaphragm valve and a first manual diaphragm valve are sequentially arranged on the sampling return sewage pipeline, and the material discharge pipeline is connected to the fermentation tank through a second sterile sampling valve, and the second sterile sampling valve is also connected to a sampling discharge sewage pipeline, and a second temperature sensor, a second pneumatic diaphragm valve and a second manual diaphragm valve are sequentially arranged on the sampling discharge sewage pipeline.

4. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 3, characterized in that: It also includes a cleaning liquid supply pipeline, which is connected to the steam supply pipeline through a first pneumatic three-way ball valve. The cleaning liquid supply pipeline is also connected to the outer cavity through a pneumatic ball valve, and a second pneumatic three-way ball valve is arranged on the clear liquid discharge pipeline.

5. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 4, characterized in that: A third pneumatic three-way ball valve is arranged on the pipeline connecting the pneumatic ball valve to the outer cavity, and the third pneumatic three-way ball valve is connected to the outer cavity exhaust pipeline.

6. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 1, characterized in that: It also includes a parameter detector, which is arranged on the clear liquid discharge pipeline and is used to detect at least one of the sugar content and the transmittance. A second pump body is arranged on the dilution water supply pipeline.

7. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 1, characterized in that: The sampling pool is connected with a waste liquid collection pool through a liquid outlet pipeline.

8. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 1, characterized in that: The sampling component includes a moving mechanism and a sampling needle, the sampling needle is connected to the moving mechanism, and the moving mechanism is electrically connected to the control main board. Under the control of the control main board, the moving mechanism drives the sampling needle to absorb the slurry from the sampling pool and move the slurry to the detection component for detection.

9. The automatic online detection and feeding automatic feedback control system for microbial fermentation process according to claim 1, characterized in that: The feeding pipeline includes a first feeding pipeline and / or a second feeding pipeline, wherein a third pneumatic diaphragm valve, a first flowmeter, a first pneumatic regulating valve and a fourth pneumatic diaphragm valve are sequentially arranged on the first feeding pipeline, and a fifth pneumatic diaphragm valve, a second flowmeter, a second pneumatic regulating valve and a sixth pneumatic diaphragm valve are sequentially arranged on the second feeding pipeline, and the first flowmeter, the first pneumatic regulating valve, the second flowmeter and the second pneumatic regulating valve are all electrically connected to the control mainboard; The control main board is configured to control the action of the first pneumatic regulating valve and / or the second pneumatic regulating valve according to the detection result of the detection component, so that the flow value of the first flow meter and / or the flow value of the second flow meter are equal to the corresponding target flow value.

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