Adjustable magnetic field device and cell sorting equipment

Abstract

The embodiment of the utility model discloses an adjustable magnetic field device and cell sorting equipment. The adjustable magnetic field device comprises a first pole, a second pole, a yoke, a coil, a control unit, a magnetic bead column mounting frame and a magnetic bead column, the first pole and the second pole are oppositely arranged, a magnetic field air gap exists between the first pole and the second pole, the polarities of the first pole and the second pole are opposite, and the yoke is connected with the first pole and the second pole; the coil is wound on the first pole and the second pole, the control unit is connected with the coil, and the control unit is used for adjusting the magnetic field intensity between the first pole and the second pole by controlling the input current of the coil; the magnetic bead column mounting frame is arranged in the middle of the magnetic field air gap and used for placing the magnetic bead columns, and the magnetic beads of different specifications are located in the magnetic bead columns. The intensity of the magnetic field is adjusted by changing the magnitude of the input current, the magnetic beads of different specifications and models can be compatible, and the cost can be reduced.

Description

Technical Field

[0001] The utility model embodiment relates to the field of cell processing technology, and in particular to an adjustable magnetic field device and a cell sorting device. Background Art

[0002] The basic principle of magnetic cell sorting is to use antibodies coupled to magnetic particles to highly specifically identify and label target cell surface antigens, and then allow the cell suspension mixed with magnetic beads to pass through a magnetic field of a certain intensity. The cells bound to the magnetic beads are adsorbed on the side walls of the magnetic field, while the unbound cells are removed to obtain purified cell targets, thereby achieving efficient sorting and enrichment.

[0003] The more famous brands of magnetic cell sorting products include German Miltenyi Biotec (MACS), Invitrogen's Dynal, BD, StemCell, R&D, etc. The size of magnetic beads varies greatly depending on the manufacturer. German Miltenyi Biotec's magnetic beads are superparamagnetic particles composed of polysaccharides and iron oxide, which are non-toxic and have a particle diameter of about 50nm; Dynal's Dynabeads magnetic beads are superparamagnetic microspheres synthesized from iron oxide magnetic materials, with three sizes, 4.5um, 2.8um and 1um in diameter; BD's IMag magnetic beads are between MACS magnetic beads and Dynabeads in size, with a diameter of about 200nm; R&D provides streptavidin-bound magnetic beads with a diameter of about 150nm, which are a bit like colloidal particles. At present, cell sorting is performed manually, and the magnetic field is fixed and cannot be adjusted. Magnetic beads of different diameters can only be used for cell sorting using the sorting magnetic field equipment of their respective companies. The magnetic field cannot be adjusted, and the equipment is not compatible with magnetic beads of different specifications and models. Utility Model Content

[0004] The utility model provides an adjustable magnetic field device and cell sorting equipment, which can adjust the strength of the magnetic field by changing the size of the input current, and can be compatible with magnetic beads of different specifications and models. There is no need to purchase corresponding equipment for magnetic beads from different manufacturers, which is conducive to reducing costs.

[0005] According to one aspect of the utility model, an adjustable magnetic field device is provided, the adjustable magnetic field device comprising: a first pole, a second pole, a yoke, a wire package, a control unit, a magnetic bead column mounting frame and a magnetic bead column;

[0006] The first pole and the second pole are arranged opposite to each other with a magnetic field air gap between them, the polarities of the first pole and the second pole are opposite, and the yoke is connected to the first pole and the second pole;

[0007] The coil is wound around the first pole and the second pole, the control unit is connected to the coil, and the control unit is used to adjust the magnetic field strength between the first pole and the second pole by controlling the input current of the coil;

[0008] The magnetic bead column mounting frame is arranged in the middle of the magnetic field air gap, and the magnetic bead column mounting frame is used to place the magnetic bead column, and magnetic beads of different specifications are located inside the magnetic bead column.

[0009] Optionally, the input current ranges from 1.2A to 1.5A, and the magnetic field strength ranges from 430mT to 620mT.

[0010] Optionally, the control unit includes: a rectifier, a phase control pre-adjuster, a variable resistance series device, and a feedback control circuit;

[0011] The first end of the rectifier is connected to the first end of the secondary winding of the transformer and the second end of the feedback control circuit, the second end of the rectifier is connected to the fourth end of the feedback control circuit, the third end of the rectifier is connected to the third end of the secondary winding of the transformer, the fourth end of the rectifier is connected to the phase control pre-adjuster and the variable resistance series device in sequence, and the first end and the fourth end of the feedback control circuit are connected to the variable resistance series device.

[0012] Optionally, the variable resistance series device includes at least one power transistor.

[0013] Optionally, the control unit also includes a capacitor and an inductor, the first end of the capacitor is connected to the phase control pre-adjuster and the variable resistance series device, the second end of the capacitor is connected to the second end of the rectifier and the fourth end of the feedback control circuit, and the inductor is connected between the third end and the fourth end of the feedback control circuit.

[0014] Optionally, the adjustable magnetic field device further includes a magnetic isolation plate, which is arranged corresponding to the coil and located outside the coil.

[0015] Optionally, the height of the magnetic bead column is 50mm-60mm.

[0016] Optionally, the diameter of the magnetic bead column ranges from 24 mm to 60 mm.

[0017] According to another aspect of the present invention, a cell sorting device is provided, and the cell sorting device comprises the adjustable magnetic field device described in any one of the above aspects.

[0018] Optionally, the cell sorting device further comprises a peristaltic pump, and the peristaltic pump is used for transporting the cell fluid.

[0019] The technical solution of the embodiment of the utility model performs cell sorting through an adjustable magnetic field device, and adjusts the strength of the magnetic field by changing the size of the input current. It can be compatible with magnetic beads of different specifications and models, and there is no need to purchase corresponding equipment for magnetic beads from different manufacturers, which is conducive to reducing costs. In summary, the utility model solves the problem that the prior art performs cell sorting manually, and the magnetic field is fixed and cannot be adjusted. It also solves the problem that magnetic beads of different diameters can only use the sorting magnetic field equipment of each company for cell sorting, the magnetic field cannot be adjusted, and the equipment is not compatible with magnetic beads of different specifications and models.

[0020] It should be understood that the contents described in this section are not intended to identify the key or important features of the embodiments of the present utility model, nor are they intended to limit the scope of the present utility model. Other features of the present utility model will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 It is a structural schematic diagram of an adjustable magnetic field device provided according to an embodiment of the utility model;

[0023] Figure 2 It is a structural schematic diagram of a magnetic bead column mounting frame and a magnetic bead column provided according to an embodiment of the utility model;

[0024] Figure 3 It is a structural schematic diagram of a magnetic bead column installation provided according to an embodiment of the utility model;

[0025] Figure 4 It is a circuit schematic diagram of a control unit provided according to an embodiment of the utility model. DETAILED DESCRIPTION

[0026] In order to enable those skilled in the art to better understand the solution of the utility model, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is only a part of the embodiment of the utility model, not all of the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the utility model.

[0027] It should be noted that the terms "first", "second", etc. in the specification and claims of the utility model and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the utility model described here can be implemented in an order other than those illustrated or described here. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0028] Figure 1 is a structural schematic diagram of an adjustable magnetic field device provided according to an embodiment of the utility model, Figure 2 It is a structural schematic diagram of a magnetic bead column mounting frame and a magnetic bead column provided according to an embodiment of the utility model. Figure 3 This is a schematic diagram of the structure of a magnetic bead column installation provided according to an embodiment of the utility model, referring to Figure 1 , Figure 2 and Figure 3 The utility model embodiment provides an adjustable magnetic field device, which includes: a first pole 10, a second pole 20, a yoke 30, a coil 40, a control unit 50, a magnetic bead column mounting frame 60 and a magnetic bead column 70; the first pole 10 and the second pole 20 are arranged opposite to each other and there is a magnetic field air gap between them, the polarities of the first pole 10 and the second pole 20 are opposite, and the yoke 30 is connected to the first pole 10 and the second pole 20; the coil 40 is wound around the first pole 10 and the second pole 20, and the control unit 50 is connected to the coil 40, and the control unit 50 is used to adjust the magnetic field strength between the first pole 10 and the second pole 20 by controlling the input current of the coil 40; the magnetic bead column mounting frame 60 is arranged in the middle of the magnetic field air gap, and the magnetic bead column mounting frame 60 is used to place the magnetic bead column 70, and magnetic beads of different specifications are located inside the magnetic bead column 70.

[0029] Specifically, the control process of cell magnetic sorting is as follows: first, magnetic beads from different manufacturers are placed inside the magnetic bead column 70, and then the magnetic bead column 70 is installed in the magnetic bead column mounting rack 60. The magnetic bead column mounting rack 60 is located in the middle of the magnetic field air gap. For magnetic beads from different manufacturers (for example, the magnetic field required for Miltenyi's magnetic beads is 450±15mT, and the magnetic field required for Xinxie Bio's magnetic beads is 600±15mT), the strength of the magnetic field is changed by changing the size of the input current, thereby achieving the adjustment of the magnetic field strength, and then the cell fluid is transported by a peristaltic pump, and then the cells are sorted when passing through the magnetic bead column 70.

[0030] The technical solution of the embodiment of the utility model performs cell sorting through an adjustable magnetic field device, and adjusts the strength of the magnetic field by changing the size of the input current. It can be compatible with magnetic beads of different specifications and models, and there is no need to purchase corresponding equipment for magnetic beads from different manufacturers, which is conducive to reducing costs. In summary, the utility model solves the problem that the prior art performs cell sorting manually, and the magnetic field is fixed and cannot be adjusted. It also solves the problem that magnetic beads of different diameters can only use the sorting magnetic field equipment of each company for cell sorting, the magnetic field cannot be adjusted, and the equipment is not compatible with magnetic beads of different specifications and models.

[0031] Optionally, the range of the input current includes 1.2A-1.5A, and the range of the magnetic field strength includes 430mT-620mT.

[0032] Specifically, the strength of the magnetic field is changed by changing the input current. An input of 1.2A can reach a magnetic field of 450±10mT, and an input of 1.5A can reach a magnetic field of 600±10mT.

[0033] Continue to refer Figure 1 Optionally, the adjustable magnetic field device further includes a magnetic isolation plate 80 , which is arranged corresponding to the coil 40 and is located outside the coil 40 .

[0034] Specifically, in order to prevent the occurrence of magnetic field disorder, a magnetic isolation sheet 80 is added to control the magnetic field within a set range and to control the uniformity of the magnetic field.

[0035] Continue to refer Figure 2 Optionally, the height of the magnetic bead column 70 is 50mm-60mm.

[0036] Continue to refer Figure 2 Optionally, the diameter of the magnetic bead column 70 includes 24mm-60mm.

[0037] Specifically, the adjustable magnetic field device is compatible with magnetic bead columns 70 of φ60 and below, and the magnetic bead column mounting frame 60 is compatible with magnetic bead columns 70 of 50mm-60mm in height and 24mm-36mm in diameter, and can adapt to different fluxes.

[0038] Figure 4 is a circuit diagram of a control unit provided according to an embodiment of the utility model, referring to Figure 4Optionally, the control unit 50 includes: a rectifier 51, a phase control pre-adjuster 52, a variable resistance series device 53, and a feedback control circuit 54; the first end of the rectifier 51 is connected to the first end of the secondary winding of the transformer and the second end of the feedback control circuit 54, the second end of the rectifier 51 is connected to the fourth end of the feedback control circuit 54, the third end of the rectifier 51 is connected to the third end of the secondary winding of the transformer, the fourth end of the rectifier 51 is connected to the phase control pre-adjuster 52 and the variable resistance series device 53 in sequence, and the first end and the fourth end of the feedback control circuit 54 are connected to the variable resistance series device 53.

[0039] Specifically, the phase control pre-adjuster 52 controls the variable resistance series device 53 to maintain a stable low voltage drop, which can minimize power consumption. The feedback control circuit 54 continuously monitors the output of the current and adjusts the serial impedance of the variable resistance series device 53 to output a continuous and stable current.

[0040] Continue to refer Figure 4 Optionally, the variable resistance series device 53 includes at least one power transistor.

[0041] Specifically, the variable resistor series device 53 is actually composed of one or more power transistors operating in a linear mode; the feedback control circuit 54 drives the output control level of the DAC to proportionally control the current value. The output end of the variable resistor series device 53 simultaneously sends a voltage to the feedback control circuit 54 to indicate that the current has been output as required.

[0042] Continue to refer Figure 4 Optionally, the control unit 50 also includes a capacitor 55 and an inductor 56, the first end of the capacitor 55 is connected to the phase control pre-adjuster 52 and the variable resistance series device 53, the second end of the capacitor 55 is connected to the second end of the rectifier 51 and the fourth end of the feedback control circuit 54, and the inductor 56 is connected between the third end and the fourth end of the feedback control circuit 54.

[0043] An embodiment of the utility model further provides a cell sorting device, which includes the adjustable magnetic field device provided by any embodiment of the utility model.

[0044] Since the cell sorting device includes the adjustable magnetic field device provided by any embodiment of the utility model, the beneficial effects of the above-mentioned cell sorting device and the adjustable magnetic field device are the same, which will not be described in detail here.

[0045] Optionally, the cell sorting device further comprises a peristaltic pump, and the peristaltic pump is used for transporting the cell fluid.

[0046] The above specific implementations do not constitute a limitation on the protection scope of the present utility model. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions can be made according to design requirements and other factors. Any modification, equivalent substitution and improvement made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. An adjustable magnetic field device, characterized in that: include: A first pole, a second pole, a yoke, a wire package, a control unit, a magnetic bead column mounting frame, and a magnetic bead column; The first pole and the second pole are arranged opposite to each other with a magnetic field air gap between them, the polarities of the first pole and the second pole are opposite, and the yoke is connected to the first pole and the second pole; The coil is wound around the first pole and the second pole, the control unit is connected to the coil, and the control unit is used to adjust the magnetic field strength between the first pole and the second pole by controlling the input current of the coil; The magnetic bead column mounting frame is arranged in the middle of the magnetic field air gap, and the magnetic bead column mounting frame is used to place the magnetic bead column, and magnetic beads of different specifications are located inside the magnetic bead column.

2. The adjustable magnetic field device according to claim 1, characterized in that: The input current ranges from 1.2A to 1.5A, and the magnetic field strength ranges from 430mT to 620mT.

3. The adjustable magnetic field device according to claim 1, characterized in that: The control unit includes: a rectifier, a phase control pre-adjuster, a variable resistance series device, and a feedback control circuit; The first end of the rectifier is connected to the first end of the secondary winding of the transformer and the second end of the feedback control circuit, the second end of the rectifier is connected to the fourth end of the feedback control circuit, the third end of the rectifier is connected to the third end of the secondary winding of the transformer, the fourth end of the rectifier is connected to the phase control pre-adjuster and the variable resistance series device in sequence, and the first end and the fourth end of the feedback control circuit are connected to the variable resistance series device.

4. The adjustable magnetic field device according to claim 3, characterized in that: The variable resistance series device includes at least one power transistor.

5. The adjustable magnetic field device according to claim 3, characterized in that: It also includes a capacitor and an inductor, wherein the first end of the capacitor is connected to the phase control pre-adjuster and the variable resistance series device, the second end of the capacitor is connected to the second end of the rectifier and the fourth end of the feedback control circuit, and the inductor is connected between the third end and the fourth end of the feedback control circuit.

6. The adjustable magnetic field device according to claim 1, characterized in that: It also includes a magnetic isolation sheet, which is arranged corresponding to the wire package and is located outside the wire package.

7. The adjustable magnetic field device according to claim 1, characterized in that: The height of the magnetic bead column is 50mm-60mm.

8. The adjustable magnetic field device according to claim 1, characterized in that: The diameter of the magnetic bead column ranges from 24mm to 60mm.

9. A cell sorting device, characterized in that: The invention comprises the adjustable magnetic field device as described in any one of claims 1 to 8.

10. The cell sorting device according to claim 9, characterized in that: Also included is a peristaltic pump, which is used to transport the cell fluid.

Images