Cell culture bottle

By designing a cell culture bottle with a rotating rod and a stirring rod, the air tightness problem caused by the opening of the bottle mouth during stirring is solved, ensuring the stability of the cell culture environment and the convenience of operation.

CN222274583UActive Publication Date: 2024-12-31SHANGHAI YIBEIRUI BIOMEDICAL SCIENCE & TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202420979691.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-08
Publication Date
2024-12-31
Estimated Expiration
2034-05-08

AI Technical Summary

Technical Problem

During the cell culture process, when stirring the culture medium, the cap of the culture bottle needs to be opened, resulting in the opening of the bottle, and the external air affects the culture environment and affects the cell culture effect.

Method used

A cell culture bottle including a bottle body, agitating bottle cap and a sealant sleeve was designed. Through a combined structure of a rotating rod and a stirring rod, the culture liquid is allowed to be stirred without opening the cover, and the sealant sleeve is used and The limit rod ensures sealing.

Benefits of technology

It is achieved to maintain the airtightness of the culture bottle during the stirring process, avoid external air exchange affecting the cell culture environment, and simplify the operation process.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to the technical field of cell culture, and discloses a cell culture bottle which comprises a bottle body, a bottle cavity is arranged in the bottle body, a bottle opening is arranged on the bottle body, the cell culture bottle further comprises a stirring bottle cap, the stirring bottle cap comprises a cap body matched with the bottle opening, a sealing rubber sleeve is arranged in the cap body in a penetrating mode, a rotating rod is connected in the sealing rubber sleeve in an inserted mode, and the rotating rod is connected with the stirring bottle cap. The lower end of the rotating rod is fixedly connected with a stirring rod matched with the bottle cavity, and stirring soft strips which are oppositely arranged and matched with the bottle cavity are arranged on the stirring rod. Compared with the prior art, the utility model has the advantage that the culture environment in the bottle cavity can be prevented from being influenced in the stirring process.
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Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture bottle. Background Art

[0002] Cell culture is the process of culturing cells in vitro in a manner that simulates the in vivo environment, allowing the cells to survive, grow, reproduce, and maintain their main structures and functions. Cell culture equipment includes porous culture plates, culture dishes, and culture bottles. When using culture bottles to culture cells, the cells need to be inoculated into the culture medium, then placed in the cell culture bottle, and finally placed in a constant temperature incubator for culture.

[0003] During the culture process, when the culture solution in the culture bottle needs to be stirred, the bottle cap needs to be opened and the culture solution needs to be stirred using a stirring rod or other tools. During this stirring process, the bottle mouth is in an open state, and the air in the culture bottle will be exchanged with the external air, making it inconvenient for the experimenter to maintain the airtightness of the culture bottle when the culture solution needs to be stirred. Utility Model Content

[0004] 1. Technical issues to be solved

[0005] The technical problem to be solved by the utility model is that the bottle cap needs to be opened to stir the culture solution. During the process, the culture bottle is in an open state, and the external air may affect the cell culture environment inside the culture bottle.

[0006] (II) Technical solution

[0007] In order to solve the above technical problems, the technical solution provided by the utility model is: a cell culture bottle, including a bottle body, a bottle cavity is provided in the bottle body, a bottle mouth is provided on the bottle body, and also includes a stirring bottle cover, the stirring bottle cover includes a cover body that cooperates with the bottle mouth, a sealing rubber sleeve is penetrated through the cover body, a rotating rod is inserted in the sealing rubber sleeve, the lower end of the rotating rod is fixedly connected to a stirring rod that cooperates with the bottle cavity, and the stirring rod is provided with stirring soft strips that are arranged relatively and cooperate with the bottle cavity.

[0008] As an improvement, a rotating knob is fixedly connected to the upper end of the rotating rod, and the rotating knob is provided with anti-slip grooves.

[0009] As an improvement, a sealing strip matching the bottle mouth is adhered to the inner wall of the cover body.

[0010] As an improvement, a limiting rod arranged opposite to the rotating rod is fixedly connected to the rotating rod, and the limiting rod is located above the cover body.

[0011] As an improvement, the sealing rubber sleeve and the cover body are bonded.

[0012] (III) Beneficial effects

[0013] Compared with the prior art, the utility model has the following advantages: after the culture solution is poured into the bottle cavity and cultured, the stirring bottle cover is assembled with the bottle body; when the culture solution needs to be stirred, the rotating knob can be turned to drive the rotating rod, the stirring rod and the stirring soft strip to rotate; the stirring soft strip can stir the culture solution; the limiting rod can prevent the rotating rod from falling too low; thus, when stirring the culture solution, there is no need to open the cover body, and the cell culture environment in the bottle cavity can be prevented from being affected during the stirring process of opening the cover body. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The utility model is a three-dimensional diagram of a bottle body of a cell culture bottle.

[0015] Figure 2 This is a schematic diagram of the structure of a stirring bottle cap for a cell culture bottle of the utility model. Figure 1 .

[0016] Figure 3 This is a schematic diagram of the structure of a stirring bottle cap for a cell culture bottle of the utility model. Figure 2 .

[0017] Figure 4 The utility model is a stereoscopic diagram of a cell culture bottle.

[0018] Figure 5 The utility model discloses a transparent diagram of a cell culture bottle.

[0019] As shown in the figure: 1. Bottle body; 2. Bottle cavity; 3. Bottle mouth; 4. Cover body; 5. Sealing rubber sleeve; 6. Turning rod; 7. Turning knob; 8. Limiting rod; 9. Stirring rod; 10. Stirring soft strip; 11. Stirring bottle cover. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0021] Embodiment 1

[0022] like Figure 1 , Figure 3 and Figure 5 As shown, a cell culture bottle includes a bottle body 1, a bottle cavity 2 is provided in the bottle body 1, a bottle mouth 3 is provided on the bottle body 1, and also includes a stirring bottle cover 11, the stirring bottle cover 11 includes a cover body 4 matched with the bottle mouth 3, and a sealing strip matched with the bottle mouth 3 is adhered to the inner wall of the cover body 4.

[0023] Through the above structure, the bottle cavity 2 can contain the culture solution, and how to achieve stirring, the specific structure is as follows:

[0024] like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, a sealing rubber sleeve 5 is penetrated through the cover body 4, and the sealing rubber sleeve 5 and the cover body 4 are adhered. A rotating rod 6 is inserted in the sealing rubber sleeve 5, and a rotating knob 7 is fixedly connected to the upper end of the rotating rod 6. The rotating knob 7 is provided with anti-slip grooves for easy rotation of the rotating rod 6. A stirring rod 9 cooperating with the bottle cavity 2 is fixedly connected to the lower end of the rotating rod 6, and a stirring soft strip 10 cooperating with the bottle cavity 2 is provided on the stirring rod 9 and arranged relatively.

[0025] With the above structure, the culture solution can be stirred without opening the cover 4 .

[0026] Embodiment 2

[0027] like Figure 1 Figure 4 As shown, a limiting rod 8 arranged opposite to the rotating rod 6 is fixedly connected to the rotating rod 6 , and the limiting rod 8 is located above the cover body 4 .

[0028] The above structure can prevent the rotating rod 6 from falling too low.

[0029] During the specific implementation of the utility model, when using a bottle body to culture cells, the culture solution is first poured into the bottle cavity, and then the cover body of the stirring bottle cover is covered on the bottle mouth. At this time, the stirring rod and the stirring soft strip are both extended into the bottle cavity. During the culture process, when the culture solution needs to be stirred, the knob is grasped and rotated, which can drive the rotating rod, the stirring rod and the stirring soft strip to rotate, thereby stirring the culture solution.

[0030] During the rotation process, the limit rod will rotate accordingly. Under the action of the limit rod, the rotating rod will not drop too much height, which can limit the rotating rod in the vertical direction. In this way, stirring can be performed without opening the cover body, which can avoid the air exchange caused by the open situation affecting the culture environment.

[0031] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0033] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. A cell culture bottle, comprising a bottle body (1), wherein the bottle body (1) is provided with a bottle cavity (2), and the bottle body (1) is provided with a bottle mouth (3), characterized in that: It also includes a stirring bottle cover (11), wherein the stirring bottle cover (11) includes a cover body (4) matched with the bottle mouth (3); A sealing rubber sleeve (5) is provided through the cover body (4), a rotating rod (6) is inserted into the sealing rubber sleeve (5), a stirring rod (9) which cooperates with the bottle cavity (2) is fixedly connected to the lower end of the rotating rod (6), and a stirring soft strip (10) which is arranged opposite to the bottle cavity (2) is provided on the stirring rod (9).

2. A cell culture bottle according to claim 1, characterized in that: The upper end of the rotating rod (6) is fixedly connected with a rotating knob (7), and the rotating knob (7) is provided with anti-slip grooves.

3. A cell culture bottle according to claim 1, characterized in that: A sealing strip that matches the bottle mouth (3) is adhered to the inner wall of the cover body (4).

4. A cell culture bottle according to claim 1, characterized in that: A limiting rod (8) arranged opposite to the rotating rod (6) is fixedly connected to the rotating rod (6), and the limiting rod (8) is located above the cover body (4).

5. A cell culture bottle according to claim 1, characterized in that: The sealing rubber sleeve (5) and the cover body (4) are bonded together.