Fluorescence immunodetection optical system
By setting up LED light sources, photodiodes and prisms in the fluorescence immunodetection optical system and adjusting the optical path, the problems of high cost of collimating lenses and large system volume in the prior art are solved, and the height and volume of the system are reduced, which is suitable for handheld Device application.
Patent Information
- Application Number
- CN202420349812.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-02-26
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2034-02-26
AI Technical Summary
In existing fluorescence immunoassay instruments, the cost of using a collimator lens to treat diffuse light as parallel light is high, and the incident light and excitation light are distributed perpendicularly, resulting in a high height and large volume, which cannot be used in handheld devices with smaller volumes.
In the fluorescence immunodetection optical system, an LED light source, a photodiode, a light shield, a second lens, a filter, a dichroic mirror and a first lens are arranged on the same line, and the optical path is adjusted through the first prism and the second prism , eliminate divergent light, retain parallel light in the center of the light source, and integrate the light source element and detection element on a circuit board.
It reduces the height and volume of the system, reduces the cost, and makes the system suitable for handheld devices, suitable for primary medical institutions and home use.
Smart Images
Figure CN222280644U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of optical detection, and in particular relates to a fluorescence immunoassay optical system. Background Art
[0002] In the fluorescent immunoassay instrument, when the optical system emits light of a specific wavelength, the test card with samples and reagents added excites light of a specific wavelength. By detecting and calculating the intensity of the excitation light, the content of specific substances in the sample can be determined, thereby achieving the purpose of diagnosing the severity of the disease.
[0003] The existing technology uses LED as the incident light source, then passes through the collimating lens to collimate the light into parallel light, then passes through the dichroic mirror to reflect the incident light, and then passes through the lens to focus and irradiate it onto the reagent card. The reagent card with the sample and reagent is irradiated with the emitted light and excites light of a specific wavelength. The excitation light passes through the lens, dichroic mirror, and filter to reach the photoelectric conversion sensor, which converts the optical signal into an electrical signal, which is output after being processed by the back-end circuit, thereby completing the detection of the concentration of a specific substance in the sample. The incident light and the excitation light are distributed vertically.
[0004] It has the following problems:
[0005] 1. The existing technology uses a collimating lens to process the divergent light emitted by the LED into parallel light, and the cost of the collimating lens is high;
[0006] 2. The incident light path and the excitation light path are vertically distributed, the detection system is tall and large, and cannot be used for smaller handheld devices. Utility Model Content
[0007] In order to overcome at least one defect (deficiency) of the above-mentioned prior art, the utility model provides a simple structure and a fluorescent immunoassay optical system which adopts a reasonable optical path design, changes the direction of the optical path, reduces the height and volume of the entire system, and reduces the cost.
[0008] In order to solve the above technical problems, the technical solution adopted by the utility model is: a fluorescent immunoassay optical system, including a base, an LED light source lamp is arranged in the internal channel of the base, a photodiode, a shading sheet and a second lens, a filter, a dichroic mirror are arranged in the internal channel of the base, and the first lens is located on the same straight line, the first prism is arranged between the LED light source and the dichroic mirror at a 90° angle, and the second prism reflection surface and the first lens are arranged at a 45° angle. At present, the optical system in the fluorescent immunoassay instrument adopts LED as the incident light source, and the light is collimated into parallel light by a collimating lens, and then the incident light is reflected by a dichroic mirror, but the cost of the collimating lens is high, and only one dichroic mirror is arranged at the same time, so that the incident light and the excitation light are vertically distributed, so that the system is high in height and large in volume, and cannot be used for smaller handheld devices, and the light source element and the detection element are separated. In this solution, an LED light source is arranged in the internal channel of the base, and a photodiode, a shading plate, a second lens, a filter, a dichroic mirror, and the first lens are arranged in the internal channel of the base on the same straight line. The first prism is arranged at a 90° angle between the LED light source and the dichroic mirror, and a 45° angle is arranged between the reflection surface of the second prism and the first lens. LED is used as the incident light source, and the center of the light source is approximately parallel light. After being reflected by the first prism, the incident light is reflected by the dichroic mirror, and then irradiated onto the reagent card through the second lens after being focused by the lens. The reagent card with the sample and the reagent is irradiated with the emitted light to excite light of a specific wavelength. The excitation light is reflected by the second prism and then passes through the lens, the dichroic mirror, the filter, and the shading plate to eliminate the divergent light. The parallel light in the center of the light source is retained and reaches the photoelectric conversion sensor, which converts the optical signal into an electrical signal, which is amplified by the back-end circuit and output, thereby completing the detection of the concentration of specific substances in the sample. By using a shading sheet to eliminate the surrounding divergent light and retain the parallel light in the center of the light source, the cost is reduced. At the same time, two prisms are added to the light channel to change the direction of the light path and the traditional vertical light path structure. The light source element and the detection element are integrated on a circuit board to reduce the height and volume of the entire system.
[0009] Furthermore, a filter is provided in the internal channel of the base, and the filter is located between the second lens and the dichroic mirror. The filter is used to eliminate stray light in the incident light, and in order to achieve a better filtering effect, the filter is provided on a straight line between the second lens and the dichroic mirror, and the filter is preferably a narrow-band filter.
[0010] Furthermore, a second prism is arranged in the inner channel of the base, and the second prism is located behind the first lens and directly above the reagent card to be detected. The first lens focuses the incident light, and the focus is on the irradiated surface of the reagent card to be detected through the second prism.
[0011] Furthermore, the inner channel of the mounting bracket is provided with filter 2, lens 3 and photoelectric collection circuit board in sequence, wherein filter 2 is located between dichroic mirror 2 and lens 3. Filter 2 is used to eliminate stray light in the excitation light, and dichroic mirror 2, filter 2, lens 3 and photoelectric collection circuit board are arranged on the same straight line. This is the best effect.
[0012] The beneficial effects of the utility model are:
[0013] 1. Adopt reasonable optical path design, eliminate the surrounding divergent light by using shading sheets, retain the parallel light in the center of the light source, and reduce costs;
[0014] 2. Add two prisms to the light receiving channel to change the direction of the light path, effectively utilize the light path space, and reduce the height and volume of the entire system;
[0015] 3. Integrate the light source element and the detection element on one circuit board to reduce the complexity of the entire system and reduce costs. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a schematic diagram of the structure of the utility model;
[0017] Figure 2 This is a schematic diagram of the three-dimensional structure of the first part of the utility model;
[0018] Figure 3 It is a schematic diagram of the three-dimensional structure of the second part of the utility model.
[0019] In the figure: 1-photodiode; 2-shading plate; 3-second lens; 4-filter; 5-dichroic mirror; 6-first lens; 7-second prism; 8-base; 9-first prism; 10-led light source; 11-circuit board. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.
[0021] In the description of the present application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.
[0022] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, unless otherwise specified, "plurality" means two or more.
[0023] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.
[0024] Example:
[0025] like Figure 1 As shown, a fluorescent immunoassay optical system includes a base 8, and the base 8 is composed of two parts, and there is no gap after the combination, such as Figure 2 and Figure 3 As shown, there are two channels inside the base 8 that can be regarded as parallel. The channels are connected inside the base 8. The outer edge of the base 8 has protrusions and depressions. After the base 8 is matched, there is a press seam structure, and no stray light will enter to interfere with the detection. For the convenience of description, the channel located at the top is named channel one, the channel located at the bottom is named channel two, and the channel that is vertically connected to channel one and channel two is named channel three, wherein the photodiode 1, the shading plate 2 and the second lens 3, the filter 4, the dichroic mirror 5, the first lens 6, and the second prism 7 are all arranged in channel one, the LED light source lamp 10, the first prism 9, are arranged in channel two in sequence, the first prism 9 is at the junction of the second channel and the third channel, and the dichroic mirror 5 is at the junction of the first channel and the third channel, wherein the photodiode 1, the shading plate 2 and the second lens 3, the filter 4, the dichroic mirror 5, the first lens 6, and the second prism 7 are arranged on the same straight line.
[0026] The installation position of the second prism 7 is exactly located at the connection point between channel 1 and the external reagent card surface. The reflection surface of the second prism 7 is at a 45° angle with the horizontal plane. The reflection surface of the first prism 9 is at the junction of the second channel and the third channel, and the outer surface of the first prism is perpendicular to the axis of the second and third channels.
[0027] Since the reagent card with the sample and the reagent needs to be irradiated with the emitted light to excite the light of a specific wavelength to enter the subsequent process, the second prism 7 and the first lens 6 are arranged in the internal channel of the base 8, and the first lens 6 is located directly behind the dichroic mirror 5. The installation position of the first lens 6 is just behind the connection between the channel 1 and the channel 3, so that the excitation light can enter the subsequent process through the first lens 6.
[0028] The scheme works as follows:
[0029] The light emitted by the LED light source 10 is reflected by the first prism 9, and then reflected by the dichroic mirror 5 to reflect the incident light, and then irradiated onto the reagent card through the second lens 7 after being focused by the first lens 6. The reagent card with the sample and the reagent is irradiated with the emitted light to excite light of a specific wavelength. The excitation light is reflected by the second prism 7 and passes through the first lens 6, the dichroic mirror 5, the filter 4, the second lens 3, the shading plate 2, and the shading plate 2 eliminates the divergent light. After retaining the parallel light at the center of the light source, it is focused on the surface of the photodiode 1 through the focusing of the second lens 3. The photodiode 1 converts the optical signal into an electrical signal, which is amplified by the back-end circuit and outputted. The signal processing and calculation are completed in cooperation with the circuit, thereby completing the detection of the concentration of a specific substance in the sample.
[0030] This solution adopts a reasonable optical system light path design. By using a shading sheet to eliminate the surrounding divergent light, retain the parallel light in the center of the light source, add two prisms to the light receiving channel, change the direction of the light path, effectively utilize the light path space, reduce the height and volume of the entire system, integrate the light source element and the detection element on a circuit board, reduce the complexity of the entire system, and can be applied to handheld devices. It is low cost and can be used in primary medical institutions and homes to reduce costs.
[0031] The above-mentioned embodiments only express the specific implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model.
Claims
1. A fluorescent immunoassay optical system, comprising a base (8), wherein an LED light source (10) is arranged in an internal channel of the base (8), characterized in that: A photodiode (1), a light shielding sheet (2), a second lens (3), a filter (4), a dichroic mirror (5), and a first lens (6) are arranged in an internal channel of the base (8) and are located on the same straight line; a first prism (9) is arranged between the LED light source (10) and the dichroic mirror (5).
2. A fluorescent immunoassay optical system according to claim 1, characterized in that: The LED light source (10) and the photodiode (1) arranged in the internal channel of the base (8) are mounted on the same circuit board (11).
3. A fluorescent immunoassay optical system according to claim 1, characterized in that: The filter (4) is arranged on a straight line between the second lens (3) and the dichroic mirror (5).
4. A fluorescent immunoassay optical system according to claim 3, characterized in that: The optical filter (4) is a narrow-band optical filter.
5. A fluorescent immunoassay optical system according to any one of claims 1 to 4, characterized in that: A first prism (9) is arranged in the internal channel of the base (8); the first prism (9) is arranged between the LED light source (10) and the dichroic mirror (5) at an angle of 90°; the dichroic mirror (5) and the reflection surface of the first prism (9) are arranged in parallel.
6. A fluorescent immunoassay optical system according to any one of claims 1 to 4, characterized in that: In the internal channel of the base (8), the reflection surface of the second prism (7) and the first lens (6) are arranged at an angle of 45°.