Automatic liquid transmission device for mass spectrum sample processing micro-fluidic chip

Abstract

The utility model discloses a mass spectrum sample processing micro-fluidic chip liquid automatic transmission device which comprises a micro-fluidic chip, a magnet, a plurality of liquid storage bottles, a plurality of electromagnetic valves, a positive pressure air pump and a controller, the micro-fluidic chip is provided with a micro-channel for injecting magnetic beads, and a plurality of liquid inlets and two outlets which are communicated with the micro-channel; the magnet is placed at the bottom of the micro-fluidic chip so as to fix the magnetic beads in the micro-channel; the plurality of liquid storage bottles are respectively connected and communicated with the plurality of liquid inlets through a plurality of first pipelines; the plurality of electromagnetic valves are respectively connected and communicated with the plurality of liquid storage bottles through a plurality of second pipelines; the plurality of electromagnetic valves are connected in parallel; the positive pressure air pump is respectively connected and communicated with a plurality of electromagnetic valves through a third pipeline; the controller is electrically connected with the multiple electromagnetic valves. According to the sample pretreatment device, the sample pretreatment automation of the element labeling ICP-MS pathogenic bacteria analysis method is realized, the operation is simple and convenient, and the sample pollution is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of mass spectrometry detection, and more specifically to an automatic liquid transmission device for a microfluidic chip for mass spectrometry sample processing. Background Art

[0002] Element-labeled inductively coupled plasma mass spectrometry is a new biological quantitative analysis method developed in recent years. It has the advantages of high detection sensitivity, strong resistance to matrix interference, simultaneous analysis of multiple targets, and easy combination with various sample pretreatment techniques. It has been successfully applied to the quantitative analysis of pathogens.

[0003] However, existing element-labeled inductively coupled plasma pathogen analysis methods all require complex and tedious manual pre-processing operations, which can easily cause blood sample contamination and produce false positive results.

[0004] Therefore, providing an automated mass spectrometry sample processing microfluidic chip liquid automatic transmission device is a problem that technicians in this field urgently need to solve. Utility Model Content

[0005] In view of this, the utility model provides a mass spectrometry sample processing microfluidic chip liquid automatic transmission device to solve the problems of cumbersome sample pretreatment operation and easy sample contamination in the existing element labeling ICP-MS pathogen analysis method.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A mass spectrometry sample processing microfluidic chip liquid automatic transmission device comprises a microfluidic chip, a magnet, a plurality of liquid storage bottles, a plurality of solenoid valves, a positive pressure air pump and a controller, wherein the microfluidic chip has a microchannel for injecting magnetic beads and a plurality of liquid inlets and two outlets communicated with the microchannel; the magnet is placed at the bottom of the microfluidic chip to fix the magnetic beads in the microchannel; the plurality of liquid storage bottles are respectively connected to the plurality of liquid inlets through a plurality of first pipes; the plurality of solenoid valves are respectively connected to the plurality of liquid storage bottles through a plurality of second pipes; the plurality of solenoid valves are connected in parallel; the positive pressure air pump is respectively connected to the plurality of solenoid valves through a third pipe; and the controller is respectively electrically connected to the plurality of solenoid valves.

[0008] By adopting the above technical solution, the beneficial effects of the utility model are:

[0009] The sample pretreatment of the element-labeled ICP-MS pathogen analysis method is automated, easy to operate, and avoids sample contamination.

[0010] Furthermore, a pressure regulating gauge is installed on the third pipeline.

[0011] Furthermore, the microchannel is serpentine or spiral.

[0012] Furthermore, the microchannel has a height of 50-1000 microns, a width of 50-1500 microns, and a length of 1-30 centimeters.

[0013] Furthermore, the number of the liquid inlets is 2-10, and correspondingly, the number of the liquid storage bottles is 2-10, and the number of the solenoid valves is 4-12.

[0014] Furthermore, the microfluidic chip is made of polymer material. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0016] Figure 1 The accompanying drawing is a schematic structural diagram of a mass spectrometry sample processing microfluidic chip liquid automatic transmission device provided by the utility model.

[0017] Figure 2 The accompanying drawing is a top view of the microfluidic chip provided by the utility model. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] Embodiment 1:

[0020] like Figure 1-2As shown, an embodiment of the utility model discloses an automatic liquid transmission device for a mass spectrometry sample processing microfluidic chip, comprising a microfluidic chip 1, a magnet 2, a plurality of liquid storage bottles 3, a plurality of solenoid valves 4, a positive pressure air pump 5 and a controller 6, wherein the microfluidic chip 1 has a microchannel 11 for injecting magnetic beads and a plurality of liquid inlets 12 and two outlets 13 connected to the microchannel 11; the magnet 2 is placed at the bottom of the microfluidic chip 1 to fix the magnetic beads in the microchannel 11; the plurality of liquid storage bottles 3 are connected to the plurality of liquid inlets 12 through a plurality of first pipes respectively; the two outlets 13 are waste liquid outlets and mass spectrometer connection ports respectively; the plurality of solenoid valves 4 are connected to the plurality of liquid storage bottles 3 through a plurality of second pipes respectively; the plurality of solenoid valves 4 are connected in parallel; the positive pressure air pump 5 is connected to the plurality of solenoid valves 4 through a third pipe respectively; the controller 6 is electrically connected to the plurality of solenoid valves 4 respectively, and a regulating valve is installed on the positive pressure air pump 5 to adjust the pressure and thus the flow rate. The utility model realizes the automation of sample pretreatment in the element labeling ICP-MS pathogenic bacteria analysis method, is easy to operate and avoids sample contamination.

[0021] In order to further optimize the technical solution of the utility model, a pressure regulating gauge 7 is installed on the third pipeline to adjust the flow rate.

[0022] Specifically, the microchannel 11 is in a serpentine or spiral shape. In this embodiment, the microchannel 11 is in a serpentine shape.

[0023] Specifically, the microchannel 11 has a height of 50-1000 microns, a width of 50-1500 microns, and a length of 1-30 centimeters. In this embodiment, the microchannel 11 has a height of 500 microns, a width of 500 microns, and a length of 15 centimeters.

[0024] Specifically, the number of liquid inlets 12 is 2-10, and correspondingly, the number of liquid storage bottles 3 is 2-10, and the number of solenoid valves 4 is 4-12. In this embodiment, the number of liquid inlets 12 is 4, and correspondingly, the number of liquid storage bottles 3 is 4, and the number of solenoid valves 4 is 6;

[0025] Specifically, the microfluidic chip 1 is made of polymer material, including but not limited to: PDMS, PMMA, PS, etc. In this embodiment, the microfluidic chip 1 is made of PDMS by soft lithography technology.

[0026] The working process of this utility model:

[0027] 1) injecting a magnetic bead suspension into the microchannel 11 of the microfluidic chip 1 and placing it above the magnet 2 to fix the magnetic beads in the microchannel 11;

[0028] 2) After the sample, cleaning solution, incubation solution and other reagents are respectively loaded into the liquid storage bottle 3, the solenoid valve 4 and the liquid storage bottle 3 are respectively connected by a plurality of second pipes; and the liquid storage bottle 3 and the liquid inlet 12 are then connected by a plurality of first pipes;

[0029] 3) Connect the positive pressure air pump 5 to the solenoid valve 4 through the third pipeline, turn on the positive pressure air pump 5, and adjust the pressure regulator 7 to the corresponding pressure;

[0030] 4) Open the solenoid valve 4 and the controller 6, so that the sample and the required reagents enter the microchannel 11 once according to the set program, and then discharge to the waste liquid or enter the injection port of the mass spectrometer.

[0031] Embodiment 2:

[0032] like Figure 1-2 As shown, the embodiment of the utility model discloses a mass spectrometry sample processing microfluidic chip liquid automatic transmission device, including a microfluidic chip 1, a magnet 2, a plurality of liquid storage bottles 3, a plurality of solenoid valves 4, a positive pressure air pump 5 and a controller 6, the microfluidic chip 1 has a microchannel 11 for injecting magnetic beads and a plurality of liquid inlets 12 and two outlets 13 connected to the microchannel 11; the magnet is placed at the bottom of the microfluidic chip 1 to fix the magnetic beads in the microchannel 11; the plurality of liquid storage bottles 3 are connected to the plurality of liquid inlets 12 through a plurality of first pipes; the two outlets 13 are waste liquid outlets and mass spectrometer connection ports respectively; the plurality of solenoid valves 4 are connected to the plurality of liquid storage bottles 3 through a plurality of second pipes; the plurality of solenoid valves 4 are connected in parallel; the positive pressure air pump 5 is connected to the plurality of solenoid valves 4 through a third pipe; the controller 6 is electrically connected to the plurality of solenoid valves 4, and a regulating valve is installed on the positive pressure air pump 5 to adjust the pressure. The utility model realizes the automation of sample pretreatment of the element labeling ICP-MS pathogen analysis method, is easy to operate, and avoids sample contamination.

[0033] In order to further optimize the technical solution of the utility model, a pressure regulating gauge 7 is installed on the third pipeline to adjust the flow rate.

[0034] Specifically, the microchannel 11 is in a serpentine or spiral shape. In this embodiment, the microchannel 11 is in a serpentine shape.

[0035] Specifically, the microchannel 11 has a height of 50-1000 microns, a width of 50-1500 microns, and a length of 1-30 centimeters. In this embodiment, the microchannel 11 has a height of 300 microns, a width of 1000 microns, and a length of 20 centimeters.

[0036] Specifically, the number of liquid inlets 12 is 2-10, and correspondingly, the number of liquid storage bottles 3 is 2-10, and the number of solenoid valves 4 is 4-12. In this embodiment, the number of liquid inlets 12 is 6, and correspondingly, the number of liquid storage bottles 3 is 6, and the number of solenoid valves 4 is 8;

[0037] Specifically, the microfluidic chip 1 is made of polymer material, including but not limited to: PDMS, PMMA, PS, etc. In this embodiment, the microfluidic chip 1 is made of PMMA through mechanical processing technology.

[0038] The working process of this utility model:

[0039] 1) injecting a magnetic bead suspension into the microchannel 11 of the microfluidic chip 1 and placing it above the magnet 2 to fix the magnetic beads in the microchannel 11;

[0040] 2) After the sample, cleaning solution, incubation solution and other reagents are respectively loaded into the liquid storage bottle 3, the solenoid valve 4 and the liquid storage bottle 3 are respectively connected by a plurality of second pipes; and the liquid storage bottle 3 and the liquid inlet 12 are then connected by a plurality of first pipes;

[0041] 3) Connect the positive pressure air pump 5 to the solenoid valve 4 through the third pipeline, turn on the positive pressure air pump 5, and adjust the pressure regulator 7 to the corresponding pressure;

[0042] 4) Open the solenoid valve 4 and the controller 6, so that the sample and the required reagents enter the microchannel 11 once according to the set program, and then discharge to the waste liquid or enter the injection port of the mass spectrometer.

[0043] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0044] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A mass spectrometry sample processing microfluidic chip liquid automatic transmission device, characterized in that: It comprises a microfluidic chip, a magnet, a plurality of liquid storage bottles, a plurality of solenoid valves, a positive pressure air pump and a controller, wherein the microfluidic chip has a microchannel for injecting magnetic beads and a plurality of liquid inlets and two outlets communicated with the microchannel; the magnet is placed at the bottom of the microfluidic chip to fix the magnetic beads in the microchannel; the plurality of liquid storage bottles are respectively connected to the plurality of liquid inlets through a plurality of first pipes; the plurality of solenoid valves are respectively connected to the plurality of liquid storage bottles through a plurality of second pipes; the plurality of solenoid valves are connected in parallel; the positive pressure air pump is respectively connected to the plurality of solenoid valves through a third pipe; and the controller is respectively electrically connected to the plurality of solenoid valves.

2. The mass spectrometry sample processing microfluidic chip liquid automatic transmission device according to claim 1, characterized in that: A pressure regulating gauge is installed on the third pipeline.

3. The mass spectrometry sample processing microfluidic chip liquid automatic transmission device according to claim 1 or 2, characterized in that: The microchannel is in a serpentine or spiral shape.

4. The mass spectrometry sample processing microfluidic chip liquid automatic transmission device according to claim 3, characterized in that: The microchannel has a height of 50-1000 microns, a width of 50-1500 microns, and a length of 1-30 centimeters.

5. The mass spectrometry sample processing microfluidic chip liquid automatic transmission device according to claim 1, characterized in that: The number of the liquid inlets is 2-10, and correspondingly, the number of the liquid storage bottles is 2-10, and the number of the solenoid valves is 4-12.

6. The mass spectrometry sample processing microfluidic chip liquid automatic transmission device according to claim 1, characterized in that: The microfluidic chip is made of polymer material.

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