Precise positioning device for magnetic rod group and magnetic rod sleeve of full-automatic nucleic acid extractor

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By using a magnetic rod group and a magnetic rod sleeve precision positioning device in a fully automatic nucleic acid extractor, axial concentric positioning of the magnetic rod group and a magnetic rod sleeve is achieved by using a rotary feeding plate and a driving motor, the magnetic rod group in the prior art is solved The problem of inaccurate control of the position of the magnetic rod sleeve in the deep-hole plate plate has improved the consistency of experimental results and sample extraction rate.

CN222294089UActive Publication Date: 2025-01-03TIANJIN MOGENETICS BIOTECH CO LTD

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Patent Information

Application Number: CN202420958584.2
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-05-07
Publication Date: 2025-01-03
Estimated Expiration: 2034-05-07

AI Technical Summary

Technical Problem

The existing fully automatic nucleic acid extractor cannot precisely control its position in the deep-hole plate hole during the assembly and debugging of the magnetic rod set and magnetic rod sleeve, resulting in poor consistency of experimental results, low sample extraction rate and low concentration sample detection. No problem.

Method used

The fully automatic nucleic acid extraction instrument magnetic rod set and magnetic rod sleeve precision positioning device is adopted to realize the axial concentric positioning of the magnetic rod set and magnetic rod sleeve through rotating feeding plate and driving motor, and use the magnetic rod set jaws and magnetic rod set to fix the plate. The precision positioning tooling completes the precise positioning of the magnetic rod and the magnetic rod sleeve.

Benefits of technology

The consistency and extraction rate of nucleic acid extraction are improved, and the situation where low-concentration samples cannot be detected is avoided, and a unified quality standard is achieved.

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Abstract

The utility model discloses a full-automatic nucleic acid extractor magnetic rod group and magnetic rod sleeve precise positioning device which comprises a base, a supporting frame is fixedly connected to one side of the top of the base, a rotary feeding disc is arranged on the other side of the top of the base, a fixing block is arranged on the side face of the supporting frame, and a magnetic rod sleeve clamping jaw is arranged on the fixing block. A magnetic bar set positioning tool is arranged at the bottom of the magnetic bar sleeve clamping jaw, and a magnetic bar sleeve positioning tool and a magnetic bar set fixing plate are sequentially arranged at the top of the magnetic bar sleeve clamping jaw. According to the utility model, the magnetic bar group and the magnetic bar sleeve are accurately positioned by utilizing the magnetic bar group and magnetic bar sleeve precise positioning device, so that the axial concentric positioning between the magnetic bar group and the magnetic bar sleeve is completed, the problem that the magnetic bar and the magnetic bar sleeve cannot be accurately positioned in the middle of a deep hole plate hole can be quickly solved, and the unified quality standard is completed; the nucleic acid extraction rate of an instrument is improved, the consistency between holes is improved, and the condition that a low-concentration sample cannot be detected is avoided.

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Description

Technical Field

[0001] The utility model relates to the technical field of full-automatic nucleic acid extractors, in particular to a magnetic rod group and a magnetic rod sleeve precision positioning device for a full-automatic nucleic acid extractor. Background Art

[0002] A fully automatic nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. For example, a Chinese patent with a publication (announcement) number of CN115747001A discloses a fully automatic nucleic acid extractor, including a main box, a middle partition is provided in the middle of the main box, and the middle partition divides the main box into an upper cavity and a lower cavity; the left, right and rear parts of the top surface of the bottom plate of the lower cavity are fixed with baffles, the sample rack is in the lower cavity and between the three baffles, and a sample tube is placed in the sample rack; a gripping mechanism is installed at the upper part of the lower cavity, and the gripper of the gripping mechanism faces the sample tube; a PCR system is constructed on the upper cavity. Fully automatic nucleic acid extractors are widely used in laboratories, and separation and purification work related to biological molecules is very important and indispensable. During the extraction process, the magnetic rod sleeve and the magnetic rod group move in the deep-well plate to achieve the steps of sample mixing and lysis, magnetic bead transfer, nucleic acid cleaning and elution. It is very important that each magnetic rod and each magnetic rod sleeve is located in the middle of each corresponding plate well in the deep-well plate, which plays a key role in the experimental results. Therefore, during the assembly and debugging process of the magnetic rod group and the magnetic rod sleeve, it is necessary to calibrate their position relative to the plate wells of the deep-well plate.

[0003] The existing technology usually relies on the machining accuracy of the parts themselves, but machining accuracy alone cannot eliminate machining and assembly errors, and the machining cost is high; or it relies on human visual observation to determine whether the magnetic rod and the magnetic rod sleeve are in the middle of the deep-well plate hole during assembly. Human visual observation has no quantitative standard and there is a lot of uncertainty. Both of the above methods cannot precisely control the position of the magnetic rod and the magnetic rod sleeve in the middle of the deep-well plate hole, which will lead to poor consistency of experimental results, low sample extraction rate, and no nucleic acid can be extracted from low-concentration samples or the extraction amount is too small, affecting the test results of the samples. Utility Model Content

[0004] The utility model aims to provide a precise positioning device for a magnetic rod group and a magnetic rod sleeve of a fully automatic nucleic acid extractor, which utilizes the precise positioning device for the magnetic rod group and the magnetic rod sleeve to precisely position the magnetic rod group and the magnetic rod sleeve, thereby completing the axial concentric positioning between the magnetic rod group and the magnetic rod sleeve, and can quickly solve the problem that the magnetic rod and the magnetic rod sleeve cannot be precisely positioned in the middle of a deep-well plate hole, thereby completing the unified quality standard, improving the nucleic acid extraction rate of the instrument, improving the consistency between holes, and avoiding the situation where low-concentration samples cannot be detected.

[0005] In order to achieve the above-mentioned purpose, the main technical solutions adopted by the utility model include:

[0006] A magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument, comprising:

[0007] A base, one side of the top of the base is fixedly connected to a support frame, the other side of the top of the base is provided with a rotating feed disc, a fixed block is provided on the side of the support frame, a magnetic rod sleeve clamp body is provided on the fixed block, a magnetic rod group positioning tooling body is provided at the bottom of the magnetic rod sleeve clamp body, a magnetic rod sleeve positioning tooling body and a magnetic rod group fixing plate are provided on the top of the magnetic rod sleeve clamp body in sequence, and the magnetic rod group fixing plate is installed on the magnetic rod sleeve clamp body through a magnetic rod group fixing seat, a magnetic rod group body is provided between the magnetic rod group fixing plate and the magnetic rod sleeve clamp body, and a deep hole plate body is provided on the rotating feed disc.

[0008] In the above-mentioned magnetic rod group and magnetic rod sleeve precision positioning device for the fully automatic nucleic acid extractor, the magnetic rod sleeve clamp body is installed on the fixed block through the magnetic rod sleeve clamp fixing seat.

[0009] In the above-mentioned magnetic rod group and magnetic rod sleeve precision positioning device for the fully automatic nucleic acid extractor, a plurality of magnetic rod sleeve bodies arranged at equal distances are arranged at the bottom of the magnetic rod sleeve clamp body.

[0010] The above-mentioned fully automatic nucleic acid extractor magnetic rod group and magnetic rod sleeve precision positioning device, wherein the top of the magnetic rod group positioning tool body is provided with a magnetic rod group positioning tool precision positioning hole, and the magnetic rod sleeve positioning tool body is provided with a magnetic rod sleeve positioning tool precision positioning hole.

[0011] In the above-mentioned precise positioning device for the magnetic rod group and magnetic rod sleeve of the fully automatic nucleic acid extractor, a driving motor for driving the rotating feeding disc is installed at the bottom of the rotating feeding disc.

[0012] In the above-mentioned fully automatic nucleic acid extractor magnetic rod group and magnetic rod sleeve precision positioning device, a deep-well plate placement area matching the deep-well plate body is provided on the rotating feeding disc and at a position corresponding to the deep-well plate body.

[0013] The utility model has at least the following beneficial effects:

[0014] The utility model realizes a precise positioning device for a magnetic rod group and a magnetic rod sleeve of a fully automatic nucleic acid extractor. The precise positioning device for the magnetic rod group and the magnetic rod sleeve is used to precisely position the magnetic rod group and the magnetic rod sleeve, thereby completing the axial concentric positioning between the magnetic rod group and the magnetic rod sleeve. The problem that the magnetic rod and the magnetic rod sleeve cannot be precisely positioned in the middle of a deep-well plate hole can be quickly solved, and a unified quality standard is completed, thereby improving the nucleic acid extraction rate of the instrument, improving the consistency between holes, and avoiding the situation where low-concentration samples cannot be detected. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0016] Figure 1 This is a structural schematic diagram of the magnetic rod group and magnetic rod sleeve precision positioning device of the fully automatic nucleic acid extraction instrument of the utility model;

[0017] Figure 2 It is a structural schematic diagram of the magnetic rod set and the magnetic rod sleeve clamping claw in the magnetic rod sleeve precision positioning device of the fully automatic nucleic acid extraction instrument of the utility model;

[0018] Figure 3 It is a structural schematic diagram of the magnetic rod group positioning tooling in the magnetic rod group and magnetic rod sleeve precision positioning device of the fully automatic nucleic acid extraction instrument of the utility model;

[0019] Figure 4 It is a structural schematic diagram of the magnetic rod sleeve positioning tooling in the magnetic rod group and magnetic rod sleeve precision positioning device of the fully automatic nucleic acid extraction instrument of the utility model.

[0020] Description of Figure Numbers:

[0021] 1. Base; 2. Support frame; 3. Rotating feed plate; 01. Magnetic rod group fixing plate; 02. Magnetic rod sleeve body; 03. Magnetic rod sleeve clamp body; 04. Magnetic rod group positioning tool body; 05. Magnetic rod sleeve positioning tool body; 06. Deep hole plate placement area; 07. Fixed block; 08. Magnetic rod group fixing seat; 09. Magnetic rod sleeve clamp seat; 10. Magnetic rod group body; 11. Deep hole plate body; 20. Magnetic rod group positioning tool precision positioning hole; 30. Magnetic rod sleeve positioning tool precision positioning hole. DETAILED DESCRIPTION

[0022] The following will describe the implementation methods of the present application in detail with the help of accompanying drawings and examples, so that the implementation process of how the present application uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0023] In the description of the embodiments of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limitations on the embodiments of the present invention.

[0024] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, an integral connection, or a detachable connection; it can be the internal connection of two components; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0025] Please refer to Figures 1 to 4 As shown, the present embodiment provides a fully automatic nucleic acid extractor magnetic rod group and magnetic rod sleeve precision positioning device, including: a base 1, a support frame 2, a rotating feed plate 3, a magnetic rod group fixing plate 01, a magnetic rod sleeve body 02, a magnetic rod sleeve clamping claw body 03, a magnetic rod group positioning tool body 04, a magnetic rod sleeve positioning tool body 05, a deep well plate placement area 06, a fixing block 07, a magnetic rod group fixing seat 08, a magnetic rod sleeve clamping claw fixing seat 09, a magnetic rod group body 10, a deep well plate body 11, a magnetic rod group positioning tool precision positioning hole 20 and a magnetic rod sleeve positioning tool precision positioning hole 30.

[0026] Please refer to Figures 1 to 4 As shown, the support frame 2 is fixedly connected to one side of the top of the base 1 , and the rotating feeding tray 3 is arranged on the other side of the top of the base 1 .

[0027] Please refer to Figures 1 to 4 As shown, the precise positioning device of the magnetic bar group body 10 and the magnetic bar sleeve body 02 is divided into two parts: a magnetic bar group positioning tool body 04 and a magnetic bar sleeve positioning tool body 05 .

[0028] Please refer to Figures 1 to 4 As shown, the magnetic bar group body 10 is assembled on the magnetic bar group fixing plate 01, and the magnetic bar group body 10 is installed through the magnetic bar group fixing seat 08, and the fixing method is bolt fixing. The assembly position of the magnetic bar group body 10 and the magnetic bar group fixing plate 01 has assembly adjustment margins reserved in the X and Y directions. The deep hole plate body 11 is placed in the deep hole plate placement area 06 of the rotating feed tray 3. There are 8 plate position areas in the rotating feed tray 3. The outer dimensions of the magnetic bar group positioning tool body 04 are consistent with the deep hole plate body 11. The precision positioning holes 20 of the magnetic bar group positioning tool are consistent with the plate hole positions at the four corners of the deep hole plate body 11. The aperture size of the precision positioning holes 20 of the magnetic bar group positioning tool is 0.2mm larger than the outer diameter of the magnetic bars in the magnetic bar group, which can achieve precise positioning of 0.1mm in the X and Y directions.

[0029] Please refer to Figures 1 to 4As shown, the magnetic rod sleeve body 02 is tightly fixed in the magnetic rod sleeve clamp body 03, the magnetic rod sleeve clamp body 03 is assembled on the fixed block 07, the magnetic rod sleeve clamp body 03 is installed through the magnetic rod sleeve clamp fixing seat 09, and the fixing method is fixed by bolts. The assembly position of the magnetic rod sleeve clamp body 03 and the fixed block 07 has assembly adjustment margins reserved in the X and Y directions. The boss of the magnetic rod sleeve positioning tool body 05 is placed in the middle groove of the magnetic rod sleeve clamp body 03, and the boss of the magnetic rod sleeve positioning tool body 05 is tightly matched with the surrounding of the middle groove of the magnetic rod sleeve clamp body 03. The four magnetic rod sleeve positioning tool precision positioning holes 30 of the magnetic rod sleeve positioning tool are in the same position as the magnetic rod sleeve body 02, and the aperture size of the magnetic rod sleeve positioning tool precision positioning hole 30 is 0.2mm larger than the outer diameter of the magnetic rod in the magnetic rod group, which can achieve accurate positioning of 0.1mm in the X and Y directions.

[0030] Please refer to Figures 1 to 4 As shown, a driving motor for driving the rotating feeding tray 3 is installed at the bottom of the rotating feeding tray 3;

[0031] By adopting the above technical solution, a driving motor is provided to facilitate the circumferential rotation of the rotating feeding disc 3, thereby realizing continuous feeding.

[0032] Please refer to Figures 1 to 4 As shown, a deep-hole plate placement area 06 matching the deep-hole plate body 11 is provided on the rotating feeding tray 3 and at a position corresponding to the deep-hole plate body 11;

[0033] By adopting the above technical solution and providing the deep-hole plate placement area 06 , the stability of the deep-hole plate body 11 placed on the rotating feeding tray 3 is increased.

[0034] The working principle of the utility model is:

[0035] Positioning and installation operation steps of the magnetic bar group body 10: pre-fix the magnetic bar group body 10 on the magnetic bar group fixing plate 01, then place the magnetic bar group positioning tool body 04 in the groove of any area of the deep hole plate placement area 06 of the turntable, push the linear motion mechanism where the magnetic bar group body 10 is located upward along the Z-axis direction, then rotate the turntable to rotate the magnetic bar group positioning tool body 04 to just below the magnetic bar group body 10, gently push the magnetic bar group body 10 downward, insert the magnetic bars at the four corners of the magnetic bar group body 10 into the magnetic bar group positioning tool precision positioning holes 20 at the four corners of the magnetic bar group positioning tool, and finally tighten the five positions of the magnetic bar group fixing seat 08 with bolts, and the positioning and installation of the magnetic bar group body 10 is completed;

[0036] The steps for positioning and installing the magnetic rod sleeve body 02 are as follows: After the magnetic rod group body 10 is positioned and installed, push the magnetic rod group upward, and then pre-fix the magnetic rod sleeve clamp body 03 on the fixed block 07, and then place the boss of the magnetic rod sleeve positioning tool body 05 in the middle groove of the magnetic rod sleeve clamp body 03, and finally push the linear motion mechanism of the magnetic rod sleeve clamp body 03 upward along the Z-axis direction, so that the four magnetic rod sleeve positioning tool precision positioning holes 30 of the magnetic rod sleeve positioning tool are inserted into the magnetic rods at the four corners of the magnetic rod group, and then tighten the four fixing bolts of the magnetic rod sleeve clamp fixing seat 09, and the positioning and installation of the magnetic rod sleeve body 02 is completed. At this point, the axial concentric positioning between the magnetic rod group body 10, the magnetic rod sleeve body 02 and the deep hole plate body 11 is completed.

[0037] The above description shows and describes several preferred embodiments of the utility model, but as before, it should be understood that the utility model is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the utility model concept of this article through the above teachings or the technology or knowledge of the relevant field. The changes and modifications made by those skilled in the art do not deviate from the spirit and scope of the utility model, and should be within the scope of protection of the claims attached to the utility model.

Claims

1. A magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument, characterized in that: include: A base (1), one side of the top of the base (1) is fixedly connected to a support frame (2), the other side of the top of the base (1) is provided with a rotating feed disc (3), a side of the support frame (2) is provided with a fixed block (07), a magnetic rod sleeve clamp body (03) is provided on the fixed block (07), a magnetic rod group positioning tool body (04) is provided at the bottom of the magnetic rod sleeve clamp body (03), a magnetic rod sleeve positioning tool body (05) and a magnetic rod group fixing plate (01) are provided on the top of the magnetic rod sleeve clamp body (03), and the magnetic rod group fixing plate (01) is installed on the magnetic rod sleeve clamp body (03) through a magnetic rod group fixing seat (08), a magnetic rod group body (10) is provided between the magnetic rod group fixing plate (01) and the magnetic rod sleeve clamp body (03), and a deep hole plate body (11) is provided on the rotating feed disc (3).

2. The magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument according to claim 1, characterized in that: The magnetic rod sleeve clamp body (03) is installed on the fixing block (07) via a magnetic rod sleeve clamp fixing seat (09).

3. A magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument according to claim 2, characterized in that: A plurality of magnetic rod sleeve bodies (02) arranged at equal distances are arranged at the bottom of the magnetic rod sleeve clamp body (03).

4. The magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument according to claim 3, characterized in that: A magnetic bar group positioning tooling precision positioning hole (20) is provided on the top of the magnetic bar group positioning tooling body (04), and a magnetic bar sleeve positioning tooling precision positioning hole (30) is provided on the magnetic bar sleeve positioning tooling body (05).

5. The magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument according to claim 4, characterized in that: A driving motor for driving the rotating feeding tray (3) is installed at the bottom of the rotating feeding tray (3).

6. The magnetic rod assembly and magnetic rod sleeve precision positioning device for a fully automatic nucleic acid extraction instrument according to claim 5, characterized in that: A deep-hole plate placement area (06) matching the deep-hole plate body (11) is arranged on the rotating feeding disc (3) and at a position corresponding to the deep-hole plate body (11).