Uric acid test paper

Abstract

The utility model relates to the field of uric acid detection, in particular to uric acid detection test paper which comprises a substrate, a detected blood sample inflow end is arranged at one end of the substrate, a current loop output end is arranged at the other end of the substrate, and a working electrode, a grounding electrode, an HCT / startup electrode and an HCT electrode are arranged at the loop output end of the substrate. The working electrode, the grounding electrode, the HCT / startup electrode and the HCT electrode are sequentially arranged in the width direction of the substrate, four contact electrodes are arranged at the detected blood sample inflow end of the substrate and connected with the working electrode, the grounding electrode, the HCT / startup electrode and the HCT electrode through wires respectively, the grounding electrode is communicated with the HCT electrode, and the grounding electrode is communicated with the HCT electrode. An insulating layer for covering the carbon layer electrode is arranged on the substrate, a reaction chamber for covering the four contact electrodes is formed in the insulating layer, and an enzyme layer is arranged in the reaction chamber. The test paper with the HCT correction function has the effect that the test paper with the HCT correction function can be used on old and new instruments at the same time.

Description

Technical Field

[0001] The present application relates to the field of uric acid detection, and in particular to a uric acid detection test strip. Background Art

[0002] The chemical formula of uric acid is C5H4N4O3. It is slightly soluble in water and easily forms crystals. The main product in normal human urine is urea, which contains a small amount of uric acid. Uric acid is the end product of purine metabolism and is a trioxypurine. At present, the main methods for testing blood uric acid are: high-performance liquid chromatography, chemiluminescence, photochemical colorimetry and direct electrochemical method. Among these methods, the detection process of high-performance liquid chromatography and chemiluminescence is cumbersome, and it takes a long time to obtain analytical results; although the photochemical colorimetry is simple to operate, the amount of blood drawn is large, the finger wound is painful, and the results are not accurate enough; only the direct electrochemical method (i.e., the electrode method) has the advantages of high sensitivity, rapid reaction, and simple operation. Uric acid test paper is required when using the direct electrochemical method to detect uric acid.

[0003] A utility model patent with publication number CN205067415U discloses a uric acid test paper, including an insulating substrate, one end of which is provided with a working electrode, a counter electrode and a reference electrode side by side and at intervals, the counter electrode is connected to the working electrode or the reference electrode, an insulating layer is covered on the working electrode, a reaction chamber is provided at the insulating layer corresponding to the working electrode, an anti-interference reagent layer is provided on the working electrode, the reagent layer is located in the reaction chamber, the reagent layer includes a water-insoluble redox electron mediator, ascorbic acid oxidase, a polymer compound and a buffer solution, a gasket is pasted around the reagent layer and a hydrophilic film is provided on the upper part thereof, a siphon channel is provided on the gasket, one end of the siphon channel is flush with the edge of the reaction chamber near the middle of the insulating layer, and the other end is flush with the end of the insulating layer and is provided with a blood collection port.

[0004] With the upgrading of detection technology, current detectors will use test strips with HCT correction function to perform HCT correction when detecting uric acid content. However, the uric acid test strips were launched too early and cannot perform HCT correction. However, after adding HCT correction to the test strips, they cannot be used on some older machines. Utility Model Content

[0005] In order to enable the test strip with HCT correction function to be used on both older and newer instruments, the present application provides a uric acid test strip.

[0006] The present application provides a uric acid test strip adopting the following technical solution:

[0007] A uric acid test strip comprises a substrate, wherein one end of the substrate is provided with a detection blood sample inflow end, the other end of the substrate is provided with a current loop output end, a working electrode, a grounding electrode, an HCT / power-on electrode and an HCT electrode are provided on the loop output end of the substrate, the working electrode, the grounding electrode, the HCT / power-on electrode and the HCT electrode are arranged in sequence along the width direction of the substrate, four contact electrodes are provided on the detection blood sample inflow end of the substrate, the four contact electrodes are respectively connected to the working electrode, the grounding electrode, the HCT / power-on electrode and the HCT electrode through wires, the grounding electrode and the HCT electrode are connected, an insulating layer for covering a carbon layer electrode is provided on the substrate, a reaction chamber covering the four contact electrodes is provided on the insulating layer, and an enzyme layer is provided in the reaction chamber.

[0008] By adopting the above technical solution, when a blood sample is tested, the blood sample enters the enzyme layer from the blood sample inflow end for reaction, thereby generating an electrical signal, which is transmitted to the working electrode, the HCT / power-on electrode and the HCT electrode through a wire, so that the blood sample has HCT correction during testing. Compared with traditional test strips, the HCT / power-on electrode has the functions of power on and off and HCT detection at the same time, so that the test strip can not only perform HCT correction, but also be suitable for older machines.

[0009] Optionally, the four contact electrodes are arranged perpendicular to the working electrode and distributed along the length direction of the substrate, and the four contact electrodes are electrically connected to the working electrode, the ground electrode, the HCT / power-on electrode and the HCT electrode in sequence along the blood sample inlet end toward the loop output end.

[0010] By adopting the above technical solution, the four contact electrodes are distributed in this position, so that the reaction chamber only needs a small volume to contact the four electrodes at the same time, which makes the volume of the enzyme layer small and plays a role in saving materials.

[0011] Optionally, the ground electrode and the HCT electrode are both silver electrodes.

[0012] By adopting the above technical solution, the properties of the silver electrode are relatively stable, the service life is extended, and the silver electrode is more sensitive.

[0013] Optionally, the HCT / power-on electrode and the HCT electrode are spaced apart and maintain a certain distance.

[0014] By adopting the above technical solution, the HCT / power-on electrode and the HCT electrode are kept at a certain distance, which has the effect of reducing interference with the HCT test.

[0015] Optionally, a gasket is provided on one end of the insulating layer away from the substrate, a siphon channel is opened on the gasket, and the siphon channel is connected to the reaction chamber.

[0016] By adopting the above technical solution, the gasket plays a role in protecting the electrode, and the siphon channel plays a role in draining the blood sample.

[0017] Optionally, a hydrophilic film layer is provided at one end of the gasket away from the insulating layer.

[0018] By adopting the above technical solution, the hydrophilic film layer plays a protective role on the test paper.

[0019] Optionally, a plurality of pores are formed on the hydrophilic membrane layer.

[0020] By adopting the above technical solution, the pores play a role of ventilation.

[0021] Optionally, the hydrophilic film layer is connected to the insulating layer via a double-sided adhesive layer.

[0022] By adopting the above technical solution, the hydrophilic film layer and the insulating layer are connected by double-sided adhesive, and the assembly is more convenient.

[0023] In summary, the present application includes at least one of the following beneficial technical effects:

[0024] 1. When testing a blood sample, the blood sample enters the enzyme layer from the blood sample inflow end for reaction, thereby generating an electrical signal, which is transmitted to the working electrode, HCT / power-on electrode and HCT electrode through a wire, so that the blood sample has HCT correction during testing. Compared with traditional test strips, the HCT / power-on electrode has the functions of power on and off and HCT detection at the same time, so that the test strip can not only perform HCT correction, but also be applicable to older machines;

[0025] 2. The four contact electrodes are arranged in parallel, so that the reaction chamber only needs a small volume to contact the four electrodes at the same time, which makes the volume of the enzyme layer small and saves materials;

[0026] 3. The properties of silver electrodes are relatively stable, which prolongs their service life. At the same time, silver electrodes are more sensitive. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the structure of a uric acid test strip according to an embodiment of the present application.

[0028] Figure 2 It is an exploded view of a uric acid test strip according to an embodiment of the present application.

[0029] Figure 3 It is a schematic diagram of the structure of a working electrode, a ground electrode, a HCT / power-on electrode and a HCT electrode of a uric acid detection test strip according to an embodiment of the present application.

[0030] Explanation of the reference numerals: 1. substrate; 11. blood sample inflow end for detection; 12. loop output end; 2. carbon layer electrode; 21. working electrode; 22. ground electrode; 23. HCT / power-on electrode; 24. HCT electrode; 25. contact electrode; 26. wire; 3. insulating layer; 31. reaction chamber; 32. enzyme layer; 4. gasket; 41. siphon channel; 5. double-sided adhesive layer; 6. hydrophilic film layer; 7. pores. DETAILED DESCRIPTION

[0031] The following is combined with Figure 1-3 This application is described in further detail.

[0032] The present application embodiment discloses a uric acid test strip. Figure 1 as well as Figure 2 A uric acid test strip comprises a substrate 1, wherein two ends of the substrate 1 are respectively a detection blood sample inlet end 11 and a loop output end 12, wherein the detection blood sample inlet end 11 is used to input a blood sample to be detected, and the loop output end 12 is used to output an electrical signal, and a working electrode 21, a grounding electrode 22, an HCT / power-on electrode 23 and an HCT electrode 24 are installed on the loop output end 12 of the substrate 1, and four contact electrodes 25 are installed on the detection blood sample inlet end 11 of the substrate 1, and the four contact electrodes 25 are respectively connected to the working electrode 21, the grounding electrode 22, the HCT / power-on electrode 23 and the HCT electrode 24 through wires 26.

[0033] Reference Figure 2 as well as Figure 3 The grounding electrode 22 and the HCT electrode 24 are both silver electrodes, so that the detection effect is more sensitive. The grounding electrode 22 and the HCT electrode 24 are connected, and the working electrode 21, the grounding electrode 22, the HCT / power-on electrode 23 and the HCT electrode 24 are arranged in sequence along the width direction of the substrate 1. The contact electrodes 25 are arranged perpendicular to the working electrode 21 and distributed along the length direction of the substrate 1. The four contact electrodes 25 are electrically connected to the working electrode 21, the grounding electrode 22, the HCT / power-on electrode 23 and the HCT electrode 24 in sequence along the detection blood sample inflow end 11 toward the loop output end 12. The HCT / power-on electrode 23 and the HCT electrode 24 are arranged at intervals and maintain a certain distance, thereby reducing interference with the HCT test.

[0034] Reference Figure 2An insulating layer 3 for covering the carbon layer electrode 2 is installed on the substrate 1. The insulating layer 3 has a protective effect on the carbon layer electrode 2. A reaction chamber 31 covering four contact electrodes 25 is provided on the insulating layer 3. An enzyme layer 32 for reacting with a blood sample is installed in the reaction chamber 31. A gasket 4 is installed at one end of the insulating layer 3 away from the substrate 1. A siphon channel 41 is provided at one end of the gasket 4 close to the blood sample inlet end 11 for detection. The siphon channel 41 is connected to the reaction chamber 31. The siphon channel 41 is used to drain the blood sample to the reaction chamber 31. The edge of the siphon channel 41 is flush with the edge of the substrate 1. A hydrophilic film layer 6 is adhered to the end of the gasket 4 away from the insulating layer 3 through a double-sided adhesive layer 5. The hydrophilic film layer 6 protects the test paper. Bubbles for ventilation are provided on the hydrophilic film layer 6.

[0035] The implementation principle of a uric acid test strip in an embodiment of the present application is as follows: when testing a blood sample, the operator turns on the instrument with the HCT / start-up electrode 23, and the blood sample flows from the blood sample inlet end 11 to the substrate 1, and the siphon channel 41 drains the blood sample to the reaction chamber 31. The blood sample reacts with the enzyme layer 32 in the reaction chamber 31, thereby generating an electrical signal, which is transmitted to the working electrode 21, the HCT / start-up electrode 23 and the HCT electrode 24 through the wire 26. The HCT / start-up electrode 23 and the HCT electrode 24 enable the blood sample to have HCT correction when the blood sample is tested. Compared with the traditional test strip, the uric acid test strip replaces the start-up electrode with the HCT / start-up electrode 23, and while retaining the original switch, the test strip has the function of HCT correction, so that the uric acid test strip can be used in newer detectors. At the same time, since the position of the electrode has not changed, the uric acid test strip can also be applied to older machines.

[0036] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A uric acid test strip, characterized in that: The invention comprises a substrate (1), wherein one end of the substrate (1) is provided with a detection blood sample inflow end (11), and the other end of the substrate (1) is provided with a current loop output end (12), and the loop output end (12) of the substrate (1) is provided with a working electrode (21), a grounding electrode (22), an HCT / power-on electrode (23), and an HCT electrode (24), wherein the working electrode (21), the grounding electrode (22), the HCT / power-on electrode (23), and the HCT electrode (24) are arranged in sequence along the width direction of the substrate (1), and the detection blood sample inflow end (11) of the substrate (1) is provided with a current loop output end (12). Four contact electrodes (25) are provided on the input end (11), and the four contact electrodes (25) are respectively connected to the working electrode (21), the ground electrode (22), the HCT / start-up electrode (23) and the HCT electrode (24) through wires (26); the ground electrode (22) and the HCT electrode (24) are arranged in communication; an insulating layer (3) for covering the carbon layer electrode (2) is provided on the substrate (1); a reaction chamber (31) covering the four contact electrodes (25) is provided on the insulating layer (3); and an enzyme layer (32) is provided in the reaction chamber (31).

2. A uric acid test strip according to claim 1, characterized in that: The four contact electrodes (25) are all arranged perpendicularly to the working electrode (21) and distributed along the length direction of the substrate (1). The four contact electrodes (25) are electrically connected to the working electrode (21), the grounding electrode (22), the HCT / power-on electrode (23), and the HCT electrode (24) in sequence along the detection blood sample inflow end (11) toward the loop output end (12).

3. A uric acid test strip according to claim 1, characterized in that: The ground electrode (22) and the HCT electrode (24) are both silver electrodes.

4. A uric acid test strip according to claim 1, characterized in that: The HCT / start-up electrode (23) and the HCT electrode (24) are arranged at intervals and maintain a certain distance.

5. A uric acid test strip according to claim 1, characterized in that: A gasket (4) is provided on one end of the insulating layer (3) away from the substrate (1), a siphon channel (41) is provided on the gasket (4), and the siphon channel (41) is connected to the reaction chamber (31).

6. A uric acid test strip according to claim 5, characterized in that: A hydrophilic film layer (6) is provided at one end of the gasket (4) away from the insulating layer (3).

7. A uric acid test strip according to claim 6, characterized in that: The hydrophilic membrane layer (6) is provided with a plurality of pores (7).

8. A uric acid test strip according to claim 7, characterized in that: The hydrophilic film layer (6) is connected to the insulating layer (3) via a double-sided adhesive layer (5).

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