Efficient box capable of being assembled and used for membrane washing or incubation

Abstract

The utility model discloses an efficient splicable box for membrane washing or incubation, which comprises a first box, tweezers fixing clamping strips are arranged on the sides, far away from each other, of the first box and a second box, splicing mechanisms are arranged on the sides, far away from the tweezers fixing clamping strips, of the first box and the second box, and a third box is arranged between the first box and the second box through the splicing mechanisms. Each of the first box, the second box and the third box comprises a cover and a box body, a bottle opening is formed in one end of each box body, a bottle cap is arranged on each bottle opening, a filter plate is arranged in one end, close to each bottle opening, of each box body, a cover is arranged at the top of the box body on one side, far away from each bottle opening, of each filter plate, and each cover is connected with the corresponding box body in a buckling manner through a sealing buckling mechanism. By arranging a series of structures, splicing and assembling among the first box, the second box and the third box are achieved, the boxes are convenient to splice, disassemble and assemble, TBST liquid can be conveniently replaced through multiple times of film washing, PVDF films are prevented from being poured out of the boxes, tweezers are conveniently fixed and taken through the tweezers fixing clamping strips, and the PVDF films can be conveniently placed and taken in the box bodies.

Description

Technical Field

[0001] The utility model relates to the technical field of membrane washing boxes, in particular to an assembleable box used for efficient membrane washing or incubation. Background Art

[0002] Protein blotting (immunoblotting test) is also known as Western Blot. It is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. It was proposed by Harry Towbin of the Friedrich Miescher Institute in Switzerland in 1979 and was first called Western Blot in Neal Burnette's Analytical Biochemistry in 1981. The steps of Western Blot experiment generally include sample preparation, protein content quantification, electrophoresis, membrane transfer (PVDF membrane or NC membrane), blocking, membrane washing, antibody incubation, chemiluminescence and other steps. The whole process takes a long time.

[0003] Currently, multiple membrane washing operations are involved after transfer and sealing operations, usually 4 to 5 times of TBST solution washing (strips), each time for 5 minutes. When multiple PVDF membranes need to be washed, the TBST solution must be frequently replaced manually to make multiple separate membrane washing boxes, and the PVDF membrane is easily poured out. Utility Model Content

[0004] The purpose of the utility model is to provide an efficient assembleable box for membrane washing or incubation, so as to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an efficient assembleable box for membrane washing or incubation, comprising box No. 1, wherein the sides of box No. 1 and box No. 2 away from each other are each provided with a tweezer fixing strip, the sides of box No. 1 and box No. 2 away from the tweezer fixing strip are provided with an assembling mechanism, a box No. 3 is provided between box No. 1 and box No. 2 via the assembling mechanism, and box No. 1, box No. 2 and box No. 3 each comprise a lid and a box body, a bottle mouth is provided at one end of the box body, a bottle cap is provided on the bottle mouth, a filter plate is provided inside the end of the box body close to the bottle mouth, a lid is provided on the top of the box body on the side of the filter plate away from the bottle mouth, and the lid is buckled and connected to the box body through a sealing buckle mechanism.

[0006] Preferably, the assembling mechanism includes connecting and fixing ridges and connecting and fixing slots, and the side of the box body of box No. 2 away from the tweezers fixing strip and the side of box No. 3 are both provided with connecting and fixing ridges, and the side of the box body of box No. 1 away from the tweezers fixing strip and the other side of box No. 3 are both provided with connecting and fixing slots, and the connecting and fixing ridges and the connecting and fixing slots are snap-connected and assembled.

[0007] Preferably, the sealing snap mechanism includes a snap plate and a snap groove, a snap plate is provided at one end of the lid away from the bottle mouth, a snap groove is provided at one end of the box body away from the bottle mouth, the snap plate and the snap groove are snap-connected, and a sealing mechanism is provided between the lid and the box body.

[0008] Preferably, the box body is made of polystyrene, the length of the box body is 10 cm, the width is 5 cm, and the height is 2.5 cm. The tweezers fixing strip is made of silk cloth.

[0009] Preferably, the filter plate is arranged in a vertical rectangular shape, and the box body between the filter plate and the bottle mouth is arranged in a V-shaped structure.

[0010] Preferably, the sealing mechanism comprises a sealing stopper and a sealing ring, the inner wall of the box body below the lid is provided with a sealing stopper, the bottom of the lid is provided with a sealing ring, the lid is buckled in the box body, and the sealing ring is sealingly provided on the sealing stopper.

[0011] Preferably, the side of the sealing stop close to the sealing ring is arranged in an L-shaped inclined surface structure, and the outer side of the lower end of the sealing ring is arranged in an inclined surface structure.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. This highly efficient assembleable box for membrane washing or incubation realizes the splicing and assembly between box No. 1, box No. 2 and box No. 3 by means of the connecting and fixing convex ridges and connecting and fixing slots arranged on the box body. The box splicing and disassembly are convenient. The filter plate and bottle mouth arranged in the box body are convenient for multiple membrane washing and replacement of TBST solution, and prevent the PVDF membrane from falling out of the box when changing the solution. The design of the tweezers fixing card strip is convenient for fixing and taking the tweezers during membrane washing operation.

[0014] 2. This highly efficient assembleable box for membrane washing or incubation has a lid arranged on the box body, and the lid and the box body are snap-fitted together through a snap plate and a snap groove, so that the placing and taking out of the PVDF membrane in the box body is convenient. The sealing ring on the lid cooperates with the sealing stopper arranged on the box body, and the lid and the box body have good snap-fitting sealing performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the structure of the No. 2 box in the utility model;

[0017] Figure 3 It is a structural schematic diagram of the box body in the utility model;

[0018] Figure 4It is a structural schematic diagram of the lid in the utility model;

[0019] Figure 5 For this utility model Figure 4 Schematic diagram of the structure enlarged at point A in the middle.

[0020] In the figure: 1. Box No. 1; 2. Box No. 2; 3. Box No. 3; 4. Lid; 41. Snap plate; 42. Snap groove; 43. Sealing stopper; 44. Sealing ring; 5. Box body; 6. Bottle mouth; 61. Bottle cap; 7. Connecting and fixing ridges; 8. Connecting and fixing grooves; 9. Tweezers fixing strip; 10. Filter plate. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] Example 1

[0025] like Figures 1 to 3As shown, the present embodiment is an efficient assembleable box for washing membranes or incubating, comprising a No. 1 box 1, a side of the No. 1 box 1 and the No. 2 box 2 away from each other is provided with a tweezers fixing clip 9, a side of the No. 1 box 1 and the No. 2 box 2 away from the tweezers fixing clip 9 is provided with an assembling mechanism, a No. 3 box 3 is provided between the No. 1 box 1 and the No. 2 box 2 through the assembling mechanism, the No. 1 box 1, the No. 2 box 2 and the No. 3 box 3 all include a cover 4 and a box body 5, one end of the box body 5 is provided with a bottle mouth 6, the bottle mouth 6 is provided with a bottle cap 61, the box body 5 is close to the bottle A filter plate 10 is provided inside one end of the bottle mouth 6, and a cover 4 is provided on the top of the box body 5 on the side of the filter plate 10 away from the bottle mouth 6. The cover 4 is buckled and connected to the box body 5 through a sealing buckle mechanism, so as to realize the splicing and assembly between the No. 1 box 1, the No. 2 box 2 and the No. 3 box 3. The boxes are convenient to splice and disassemble. The filter plate 10 and the bottle mouth 6 arranged in the box body 5 are convenient for multiple membrane washing and replacement of TBST solution, and prevent the PVDF membrane from falling out of the box when changing the solution. The design of the tweezers fixing strip 9 is convenient for fixing and taking the tweezers during the membrane washing operation.

[0026] Specifically, the assembling mechanism includes a connecting and fixing rib 7 and a connecting and fixing slot 8. The side of the box body 5 of box No. 2 box 2 away from the tweezers fixing strip 9 and the side of box No. 3 box 3 are both provided with a connecting and fixing rib 7. The side of the box body 5 of box No. 1 box 1 away from the tweezers fixing strip 9 and the other side of box No. 3 box 3 are both provided with a connecting and fixing slot 8. The connecting and fixing rib 7 and the connecting and fixing slot 8 are assembled by clamping, so assembly and disassembly are convenient.

[0027] Furthermore, the sealing snap-fit ​​mechanism includes a snap-fit ​​plate 41 and a snap-fit ​​groove 42. The snap-fit ​​plate 41 is provided at one end of the lid 4 away from the bottle mouth 6, and the snap-fit ​​groove 42 is provided at one end of the box body 5 away from the bottle mouth 6. The snap-fit ​​plate 41 and the snap-fit ​​groove 42 are snap-fitted. A sealing mechanism is provided between the lid 4 and the box body 5. The lid 4 and the box body 5 are snap-fitted together, so that the lid is stably closed and easy to open.

[0028] Furthermore, the box body 5 is made of polystyrene, which is hard and not easy to bend. The box body 5 is 10 cm long, 5 cm wide and 2.5 cm high. The tweezers fixing strip 9 is made of silk cloth strip with a length of 2-3 cm, which is convenient for placing the tweezers on the tweezers fixing strip 9.

[0029] Furthermore, the filter plate 10 is arranged in a vertical rectangular shape, and the box body 5 between the filter plate 10 and the bottle mouth 6 is arranged in a V-shaped structure, which is convenient for liquid replacement and less residue.

[0030] The method of using this embodiment is as follows: by engaging the connecting and fixing convex rib 7 on one side of the No. 3 box 3 with the connecting and fixing groove 8 on the No. 1 box 1, the No. 3 box 3 is buckled and assembled with the No. 1 box 1. Similarly, the connecting and fixing convex rib 7 on the No. 2 box 2 is engaged with the connecting and fixing groove 8 on the No. 3 box 3, and the No. 2 box 2 is buckled and assembled with the No. 3 box 3. Similarly, the connecting and fixing convex rib 7 of the No. 2 box 2 can be directly engaged with the connecting and fixing groove 8 of the No. 1 box 1, and the No. 1 box 1 is assembled with the No. 2 box 2. Open the cover 4 on the box body 5, put the PVDF membrane and the TBST solution for washing the membrane into the interior of the box body 5, cover the cover 4 with the box body 5, and The snap plate 41 is snapped into the snap groove 42 on the box body 5, which is convenient for placing and taking out the PVDF membrane in the box body 5. The utility model realizes the splicing and assembly between the No. 1 box 1, the No. 2 box 2 and the No. 3 box 3. The box splicing and disassembly are convenient. By unscrewing the bottle cap 61 on the bottle mouth 6, the membrane washing liquid in the box body 5 can be poured out and replaced. When replacing, the filter plate 10 blocks the PVDF membrane in the box body 5 from being poured out from the bottle mouth 6 along with the replacement liquid. That is, the filter plate 10 and the bottle mouth 6 arranged in the box body 5 are convenient for multiple membrane washing and replacement of TBST liquid, and prevent the PVDF membrane from pouring out of the box when changing the liquid. The design of the tweezers fixing strip 9 is convenient for fixing and taking with tweezers during the membrane washing operation.

[0031] Example 2

[0032] The structure of the efficient assembleable box for washing membrane or incubation in this embodiment is basically the same as the structure of the efficient assembleable box for washing membrane or incubation in embodiment 1, except that the sealing mechanism includes a sealing stop 43 and a sealing ring 44 (see Figures 1 to 5 A sealing stop 43 is provided on the inner wall of the box body 5 below the cover 4, and a sealing ring 44 is provided at the bottom of the cover 4. The cover 4 is buckled in the box body 5, and the sealing ring 44 is sealed on the sealing stop 43. The sealing ring 44 and the sealing stop 43 are both made of sealing rubber material. The sealing ring 44 is stuck on the sealing stop 43 to seal the cover between the cover 4 and the box body 5.

[0033] Specifically, the side of the sealing baffle 43 close to the sealing ring 44 is arranged in an L-shaped inclined surface structure, that is, the side of the sealing baffle 43 close to the sealing ring 44 is an inclined surface structure with a high outside and a low inside. The high outside and the low inside can block water and reduce the probability of infiltration. The outer side of the lower end of the sealing ring 44 is arranged in an inclined surface structure. The inclined surface structure of the sealing ring 44 and the inclined surface structure of the sealing baffle 43 have the same inclination angle, and the structure is compact and the sealing performance is better.

[0034] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. An efficient assembleable box for membrane washing or incubation, comprising a box No. 1 (1), characterized in that: A tweezer fixing clip (9) is provided on the side away from the first box (1) and the second box (2), and an assembly mechanism is provided on the side away from the tweezer fixing clip (9). A third box (3) is provided between the first box (1) and the second box (2) via the assembly mechanism. The first box (1), the second box (2) and the third box (3) each comprise a cover (4) and a box body (5). A bottle mouth (6) is provided at one end of the box body (5), and a bottle cover (61) is provided on the bottle mouth (6). A filter plate (10) is provided inside the box body (5) at one end close to the bottle mouth (6), and a cover (4) is provided on the top of the box body (5) at the side of the filter plate (10) away from the bottle mouth (6). The cover (4) is buckled and connected to the box body (5) via a sealing buckle mechanism.

2. An efficient assembleable box for membrane washing or incubation according to claim 1, characterized in that: The assembling mechanism comprises a connecting and fixing convex rib (7) and a connecting and fixing slot (8); a side of the box body (5) of the No. 2 box (2) away from the tweezers fixing strip (9) and a side of the No. 3 box (3) are both provided with a connecting and fixing convex rib (7); a side of the box body (5) of the No. 1 box (1) away from the tweezers fixing strip (9) and a side of the No. 3 box (3) are both provided with a connecting and fixing slot (8); the connecting and fixing convex rib (7) and the connecting and fixing slot (8) are connected and assembled by snapping.

3. An efficient assembleable box for membrane washing or incubation according to claim 1, characterized in that: The sealing snap-fit ​​mechanism comprises a snap-fit ​​plate (41) and a snap-fit ​​groove (42); the snap-fit ​​plate (41) is provided at one end of the cover (4) away from the bottle mouth (6); the snap-fit ​​groove (42) is provided at one end of the box body (5) away from the bottle mouth (6); the snap-fit ​​plate (41) and the snap-fit ​​groove (42) are snap-fitted to each other; and a sealing mechanism is provided between the cover (4) and the box body (5).

4. An efficient assembleable box for membrane washing or incubation according to claim 1, characterized in that: The box body (5) is made of polystyrene, and has a length of 10 cm, a width of 5 cm, and a height of 2.5 cm. The tweezer fixing strip (9) is made of a silk cloth strip.

5. An efficient assembleable box for membrane washing or incubation according to claim 1, characterized in that: The filter plate (10) is arranged in a vertical rectangular shape, and the box body (5) between the filter plate (10) and the bottle mouth (6) is arranged in a V-shaped structure.

6. An efficient assembleable box for membrane washing or incubation according to claim 3, characterized in that: The sealing mechanism comprises a sealing stopper (43) and a sealing ring (44); the inner wall of the box body (5) below the cover (4) is provided with the sealing stopper (43); the bottom of the cover (4) is provided with the sealing ring (44); the cover (4) is buckled and arranged in the box body (5); and the sealing ring (44) is sealingly arranged on the sealing stopper (43).

7. An efficient assembleable box for membrane washing or incubation according to claim 6, characterized in that: The side of the sealing stop (43) close to the sealing ring (44) is arranged in an L-shaped inclined surface structure, and the outer side of the lower end of the sealing ring (44) is arranged in an inclined surface structure.

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