Fixing device for in-vitro implant adsorption cell test

Abstract

The utility model relates to the technical field of cell adsorption capacity verification of an implant, and discloses a fixing device for an in-vitro implant cell adsorption test, which comprises a transparent container, a top cover detachably mounted at the upper end of the transparent container, a bottom cover detachably mounted at the bottom end of the transparent container, and an implant, the implant is detachably mounted in the transparent container. The implant is fixed at the bottom of the device, the position of the top cover is adjusted according to the size of the implant, agar with a certain concentration is injected from the injection port in the top cover and completely wraps the periphery of the implant, the top of the implant is tightly attached to the groove in the bottom side of the top cover and cannot be wrapped by the agar, and after the agar is completely solidified, the top of the implant is sealed. The implant wrapped with the agar can be taken out for testing, and the implant with the exposed area of 1mm * 1mm is obtained.

Description

Technical Field

[0001] The utility model relates to the technical field of verification of the ability of implants to absorb cells, in particular to a fixing device for an in vitro implant absorption cell test. Background Art

[0002] There are two modes of new bone growth at the bone interface of oral implants: distal osteogenesis and contact osteogenesis. It is generally believed that contact osteogenesis can better explain the ideal state of the bone bonding interface. Osteoblasts are the main functional cells of bone formation, which secrete osteoid (unmineralized organic components of bone matrix). With the deposition and mineralization of osteoid, osteoblasts sink into bone pits and become bone lining cells or undergo apoptosis. The process of contact osteogenesis is that osteoblasts first attach, adhere, and secrete bone matrix on the surface of the implant, thereby initiating the direct generation of new bone on the implant surface. The new bone gradually grows and extends from the implant surface to the bone wound surface. The adsorption and diffusion of osteoblasts on the implant surface is the key to contact osteogenesis, which can affect the ability of cells to proliferate and differentiate when in contact with the implant.

[0003] Since the implant bone integration theory was proposed by Swedish scientist Professor Branemark in 1965, oral implant restoration technology has developed rapidly. By the end of 2022, the overall number of registered oral implant system medical device products in my country has been on the rise, and the proportion of domestic oral implant system medical device products has increased year by year, and in 2022, the proportion of domestic products has exceeded half. At present, domestic hydrophilic titanium materials, titanium zirconium materials and other modified material implants have not been included in the centralized procurement, and more and more people will turn to this direction for research, hoping to prove the superiority of implant product material modification through experiments, including the verification of the product's ability to adsorb osteoblasts.

[0004] In the in vitro implant adsorption osteoblast test, finished implants are often used for the test. The shape of the implant is a column with irregular threads on the surface, and only a small part of the top is smooth. In addition, the shapes of implants produced by different manufacturers are not the same. If we want to compare with raw materials or products already on the market, we must know the number of cells adsorbed by the implant in the same unit area to make a more accurate analysis. There is currently no literature on how to determine the test area of ​​the finished implant. Therefore, we provide a fixture for in vitro implant adsorption cell test to solve the above problem. Utility Model Content

[0005] The utility model aims to provide a fixing device for in vitro implant cell adsorption test, which solves the problems raised in the background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a fixing device for an in vitro implant cell adsorption test, comprising a transparent container, a top cover being detachably mounted on the upper end of the transparent container, a bottom cover being detachably mounted on the bottom end of the transparent container, and an implant being detachably mounted inside the transparent container, a groove being fixedly connected to the middle of the inner wall at the top end of the top cover, the dimensions of the groove being 1 mm in length*1 mm in width*2 mm in height, a positioning block being fixedly connected to the middle of the upper surface of the bottom cover, the positioning block being movably connected to the bottom end of the implant, and the groove being movably connected to the top end of the implant.

[0007] As an optional solution of the technical solution of the present application, an exhaust hole and a filling port are formed through the upper surface of the top cover, a handle is fixedly installed in the middle of the upper surface of the top cover, and the exhaust hole and the filling port are respectively placed on both sides of the handle.

[0008] As an optional solution of the technical solution of the present application, the inner wall of the top of the transparent container is provided with an internal thread, the outer side of the top cover is provided with an external thread, and the top cover is threadedly connected to the transparent container.

[0009] As an optional solution of the technical solution of the present application, the outer side of the lower end of the transparent container is provided with an external thread, the inner wall of the bottom cover is provided with an internal thread, and the bottom cover is threadedly connected to the transparent container.

[0010] As an optional solution of the technical solution of the present application, the positioning block is a cone, the top cover and the bottom cover are made of medical silicone, and the transparent container is made of high borosilicate glass.

[0011] Compared with the prior art, the beneficial effects of the utility model are as follows: the implant is fixed at the bottom of the device, the position of the top cover is adjusted according to the size of the implant and then fixed, agar of a certain concentration is injected from the filling port on the top cover to completely wrap around the implant, and the top of the implant fits tightly with the groove on the bottom side of the top cover and will not be wrapped by the agar. After the agar is completely solidified, the implant wrapped in agar can be taken out for testing, and an implant with an exposed area of ​​1mm*1mm is obtained. In the subsequent test process, cells will only grow on the exposed area. This solves the problem of comparing the number of cells adsorbed by different implants under the same unit area. In addition, this device is reusable, uses cheap materials, and has a high cost performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Other features, objects and advantages of the present invention will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0013] Figure 1 This is a schematic diagram of the structure of a fixing device for in vitro implant cell adsorption test according to the utility model;

[0014] Figure 2 This is a front view of a fixing device for in vitro implant cell adsorption test according to the utility model.

[0015] In the figure: 1. transparent container; 11. top cover; 12. bottom cover; 13. positioning block; 14. implant; 15. groove; 2. exhaust hole; 21. filling port; 22. handle. DETAILED DESCRIPTION

[0016] See also Figure 1-Figure 2 The utility model provides a technical solution: a fixing device for an in vitro implant cell adsorption test, comprising a transparent container 1, a top cover 11 being detachably mounted on the upper end of the transparent container 1, a bottom cover 12 being detachably mounted on the bottom end of the transparent container 1, and an implant 14 being detachably mounted inside the transparent container 1, a groove 15 being fixedly connected to the middle of the inner wall at the top end of the top cover 11, the size of the groove 15 being 1 mm long*1 mm wide*2 mm high, a positioning block 13 being fixedly connected to the middle of the upper surface of the bottom cover 12, the positioning block 13 being movably connected to the bottom end of the implant 14, the groove 15 being movably connected to the top end of the implant 14, the positioning block 13 being a cone, the top cover 11 and the bottom cover 12 being made of medical silica gel, and the transparent container 1 being made of high borosilicate glass.

[0017] In this technical solution, the conical positioning block 13 in the center of the bottom cover 12 can fix the implant 14 so that it does not move. The groove 15 with a length of 1mm, a width of 1mm and a height of 2mm in the middle of the bottom side of the top cover 11 is used to determine the exposure area of ​​the implant 14. The transparent container 1 is made of high borosilicate glass, so that it can be observed whether the implant 14 moves in the container and the amount of agar injected.

[0018] In this embodiment, the upper surface of the top cover 11 is penetrated with an exhaust hole 2 and a filling port 21 , a handle 22 is fixedly mounted in the middle of the upper surface of the top cover 11 , and the exhaust hole 2 and the filling port 21 are respectively disposed on both sides of the handle 22 .

[0019] In this technical solution, the handle 22 allows the staff to conveniently rotate the top cover 11 to be threadedly connected to the top of the transparent container 1 , and agar of a certain concentration can be conveniently added into the transparent container 1 through the filling port 21 .

[0020] In this embodiment, an inner wall of the top of the transparent container 1 is provided with an internal thread, an outer side of the top cover 11 is provided with an external thread, the top cover 11 is threadedly connected to the transparent container 1, an outer side of the lower end of the transparent container 1 is provided with an external thread, an inner wall of the bottom cover 12 is provided with an internal thread, and the bottom cover 12 is threadedly connected to the transparent container 1.

[0021] In this technical solution, it is convenient for the staff to disassemble the top cover 11 and the bottom cover 12 , and further convenient to take out the implant 14 inside the transparent container 1 .

[0022] When using a fixing device for an in vitro implant cell adsorption test, first tighten the bottom cover 12 to the bottom end of the transparent container 1, then put the implant 14 into the transparent container 1, insert the positioning block 13 in the middle of the upper surface of the bottom cover 12 into the bottom end of the implant 14, and position the implant 14. Then, rotate the top cover 11 through the handle 22 to connect it to the top of the transparent container 1, and twist the position of the top cover 11 according to the size of the implant 14 so that the groove 15 in the middle of the bottom side of the top cover 11 completely fits the implant 14. Then, inject agar of a certain concentration. After the agar solidifies, open the top cover 11 and the bottom cover 12, and take out the implant 14 wrapped with agar on all sides, so that an implant 14 with an exposed area of ​​1mm*1mm can be obtained for subsequent tests.

Claims

1. A fixing device for an in vitro implant cell adsorption test, comprising a transparent container (1), wherein a top cover (11) is detachably mounted on the upper end of the transparent container (1), and a bottom cover (12) is detachably mounted on the lower end of the transparent container (1), wherein: The invention also comprises an implant (14), wherein the implant (14) is detachably mounted inside the transparent container (1); a groove (15) is fixedly connected to the middle of the inner wall at the top end of the top cover (11); the size of the groove (15) is 1 mm in length*1 mm in width*2 mm in height; a positioning block (13) is fixedly connected to the middle of the upper surface of the bottom cover (12); the positioning block (13) is movably connected to the bottom end of the implant (14); and the groove (15) is movably connected to the top end of the implant (14).

2. The fixing device for in vitro implant cell adsorption test according to claim 1, characterized in that: An exhaust hole (2) and a filling port (21) are formed through the upper surface of the top cover (11), a handle (22) is fixedly mounted in the middle of the upper surface of the top cover (11), and the exhaust hole (2) and the filling port (21) are respectively disposed on both sides of the handle (22).

3. The fixing device for in vitro implant cell adsorption test according to claim 1, characterized in that: The inner wall of the top end of the transparent container (1) is provided with an internal thread, the outer side of the top cover (11) is provided with an external thread, and the top cover (11) is threadably connected to the transparent container (1).

4. The fixing device for in vitro implant cell adsorption test according to claim 1, characterized in that: The outer side of the lower end of the transparent container (1) is provided with an external thread, the inner wall of the bottom cover (12) is provided with an internal thread, and the bottom cover (12) is threadably connected to the transparent container (1).

5. The fixing device for in vitro implant cell adsorption test according to claim 1, characterized in that: The positioning block (13) is a cone, the top cover (11) and the bottom cover (12) are made of medical silica gel, and the transparent container (1) is made of high borosilicate glass.

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