Quick sealing device for 96-well plate

Abstract

The utility model discloses a quick sealing device for a 96-well plate, which belongs to the field of 96-well plates and comprises a gland covering the 96-well plate and a sealing mechanism arranged on the inner side of the gland and corresponding to the upper position of a reaction tube of the 96-well plate. The sealing mechanism comprises a pressure head, a sleeve and a first spring; the pressure head moves close to or far away from the reaction tube above the reaction tube; the sleeve sleeves the outer part of one end, far away from the reaction tube, of the pressure head and is fixedly connected with the gland; the first spring sleeves the outer part of the pressure head, and two ends of the first spring are respectively connected with the pressure head and the sleeve; the three arc-shaped clamping jaws are hinged to the side face of the pressing head and distributed in an annular array mode. According to the rapid sealing device for the 96-well plate, simultaneous sealing of multiple reaction tubes is achieved through combination of the gland and the sealing mechanism, the sealing efficiency is improved, film covering can be conducted on the whole reaction tubes, the trouble of independent film covering one by one is avoided, operation is simplified, and the sealing efficiency is further improved.

Description

Technical Field

[0001] The utility model relates to the field of 96-well plates, in particular to a 96-well plate rapid sealing device. Background Art

[0002] The 96-well plate is a versatile experimental tool that plays a key role in enzyme-linked immunosorbent assay (ELISA) and participates in immune responses such as antibody or antigen coating, buffer blocks, etc. It is widely used in many fields such as molecular biology, cell biology, enzymology, drug development, etc., with multiple functions and applications.

[0003] The Chinese utility model patent with publication number CN216472145U discloses a 96-well plate rapid sealing device; the sealing device shrinks or expands the clamping position by compressing and relaxing the spring component. After the reaction tube is glued with the sealing film, the device is sleeved on the reaction tube, and the front clamping plate and the rear clamping plate are held on both sides to compress the spring component. The clamping position will tightly glue the sealing film. It can also be used on a 96-tube plate for rapid sealing, that is, eight clamping positions are provided, and the interval distances thereof correspond to the eight connected tubes. The operation can be carried out in batches, which saves time and effort, and is simple to operate. The sealing film is evenly stressed and tightly sealed.

[0004] However, the sealing device still needs to first bond the sealing film to each reaction tube opening individually, and then use the device to clamp the sealing film, and can only seal eight seals at a time, so improving the overall sealing efficiency is limited and there is still room for improvement.

[0005] Therefore, a 96-well plate rapid sealing device is provided to solve the above problems. Utility Model Content

[0006] 1. Technical issues to be solved

[0007] The utility model provides a 96-well plate rapid sealing device, aiming to solve the problem of low overall sealing efficiency of the existing sealing device proposed in the background technology.

[0008] (II) Technical solution

[0009] To achieve the above purpose, the utility model provides the following technical solution: a 96-well plate rapid sealing device, comprising a pressure cover for covering the well plate and a sealing mechanism arranged on the inner side of the pressure cover at a position above the reaction tube of the well plate corresponding to the well plate.

[0010] The sealing mechanism includes a pressure head that moves above the reaction tube and approaches or moves away from the reaction tube, a sleeve that is sleeved outside the pressure head at one end away from the reaction tube and fixedly connected to the pressure cover, a first spring that is sleeved outside the pressure head and whose two ends are respectively connected to the pressure head and the sleeve, three arc-shaped clamping jaws that are hinged on the side of the pressure head and distributed in a ring array, and a spring sheet that is fixedly connected to the side of the pressure head at one end and contacts the inner side of the arc-shaped clamping jaw at the other end, the three arc-shaped clamping jaws form a ring sleeved on the port of the reaction tube, wherein the end of the sleeve close to the pressure head has a tube skirt that is sleeved outside the ring and is used to squeeze the arc-shaped clamping jaws to retract inward; after the entire sealing film is covered on the orifice plate at one time, the pressure head is moved above the reaction tube close to the reaction tube by pressing the pressure cover. reaction tube, when the pressure head approaches the reaction tube, the first spring is compressed, and the reaction force presses the sealing film area on the port of the reaction tube, and as the pressure head approaches the reaction tube, the arc-shaped clamping claw under the action of the spring sheet maintains an outward expansion shape, and the sealing film larger than the range of the reaction tube port is divided, and as the pressure head continues to be pressed down, the pressure head does not move, so that the sleeve continues to approach the reaction tube, and as the sleeve moves downward, the outer wall of the arc-shaped clamping claw is squeezed until the arc-shaped clamping claw is completely retracted to form a ring, so that the edge of this part of the sealing film is covered on the outer wall of the reaction tube port, so as to achieve rapid sealing, and when the sleeve is away from the reaction tube, the first spring releases energy to reset the pressure head, and the ring is also opened at the same time, which is convenient for the next sealing operation, and through the combination of the pressure cover and the sealing mechanism, the simultaneous sealing of multiple reaction tubes in the orifice plate is achieved, which greatly improves the sealing efficiency.

[0011] Preferably, in order to prevent the reaction tube port from being contacted by hard objects and to prevent the sealing film from being scratched, the end of the pressure head away from the sleeve is fixedly connected to a pressure pad; when the pressure head approaches the reaction tube, it first contacts the upper end of the reaction tube to play a buffering and protective role.

[0012] Preferably, in order to make the sealing film fit more closely to the outer wall of the reaction tube, a clamping pad is fixedly connected to one side of the arc-shaped clamping jaw close to the pressure head; when the arc-shaped clamping jaw is retracted inward and wraps the port of the reaction tube, the clamping pad first contacts the port of the reaction tube, thereby playing a role in buffering and enhancing friction.

[0013] Preferably, in order to prevent the sealing film from being stirred by the pressure head, the pressure head is slidably connected to the sleeve; the sliding connection allows the pressure head to remain stable and not rotate when moving on the sleeve, thereby preventing the sealing film from being stirred and dislocated.

[0014] Preferably, in order to facilitate the positioning of the gland during sealing so that each set of rings can be aligned with the reaction tube port, the gland has a skirt that engages with the orifice plate at a position corresponding to the edge of one side of the orifice plate close to the orifice plate, and the height of the skirt is less than the height of the orifice plate; when the gland is placed on the orifice plate, the skirt will first contact and slightly engage with the edge of the orifice plate, providing a stable positioning reference for the gland, and at the same time, because the height of the skirt is less than the height of the orifice plate, it can provide movement space for the gland, thereby facilitating subsequent smooth operation.

[0015] Preferably, in order to facilitate the removal of the sealing device after the sealing is completed, a rebound mechanism is provided on the skirt, and the rebound mechanism includes a telescopic leg that moves closer to or away from the orifice plate on the skirt, a foot pad fixedly connected to the end of the telescopic leg away from the skirt, and a second spring that is sleeved on the outside of the telescopic leg and whose two ends are respectively connected to the skirt and the foot pad, wherein the telescopic leg is slidably connected to the skirt; when the pressure cover is pressed down to seal, the second spring is squeezed by the bottom edge of the orifice plate, thereby releasing the pressed pressure cover after the sealing is completed, so that the pressure cover is squeezed back by the elastic force of the second spring, thereby separating the pressure cover from the orifice plate for easy removal.

[0016] (III) Beneficial effects

[0017] The 96-well plate rapid sealing device combines the capping and sealing mechanisms to achieve simultaneous sealing of multiple reaction tubes, thereby improving the sealing efficiency. The entire plate can be coated with film, eliminating the trouble of coating each plate individually, thus simplifying the operation and further improving the sealing efficiency.

[0018] The 96-well plate quick sealing device has a pressure pad and a clamping pad, which protect the reaction tube and avoid damage, and at the same time enhances the tightness of the sealing.

[0019] The 96-well plate quick sealing device, the setting of the skirt and the rebound mechanism ensure the stable positioning between the pressure cover and the well plate, and prevent deviation or shaking during the sealing process. These beneficial effects not only improve the accuracy and efficiency of experimental operations, but also extend the service life of the reaction tube, bringing great convenience and reliability to laboratory work. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of a 96-well plate rapid sealing device;

[0021] Figure 2 for Figure 1 The enlarged structural diagram at A in the middle;

[0022] Figure 3 It is a schematic diagram of the exploded structure of a sealing mechanism in a 96-well plate rapid sealing device;

[0023] Figure 4 The figure is a schematic diagram of the structure inside the sleeve of a 96-well plate rapid sealing device.

[0024] In the figure:

[0025] 1. gland; 11. skirt;

[0026] 2. Sealing mechanism;

[0027] 21. Pressing head; 211. Pressing pad; 22. Sleeve; 221. Tube skirt; 23. First spring; 24. Holding ring; 241. Arc-shaped clamping claw; 242. Clamping pad; 25. Shrapnel;

[0028] 3. Rebound mechanism; 31. Telescopic leg; 32. Foot pad; 33. Second spring. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] Example 1

[0031] See also Figure 1-Figure 4 The utility model provides a technical solution: a 96-well plate rapid sealing device, comprising a pressure cover 1 for covering the well plate and a sealing mechanism 2 arranged at a position above the reaction tube of the well plate corresponding to the inner side of the pressure cover 1.

[0032] The sealing mechanism 2 includes a pressure head 21 that moves above the reaction tube and moves closer to or away from the reaction tube, a sleeve 22 that is sleeved on the outside of one end of the pressure head 21 away from the reaction tube and fixedly connected to the pressure cover 1, a first spring 23 that is sleeved on the outside of the pressure head 21 and whose two ends are respectively connected to the pressure head 21 and the sleeve 22, three arc-shaped clamping jaws 241 that are hinged on the side of the pressure head 21 and distributed in a ring array, and a spring piece 25 that has one end fixedly connected to the side of the pressure head 21 and the other end in contact with the inner side of the arc-shaped clamping jaws 241. The three arc-shaped clamping jaws 241 form a ring 24 that is sleeved on the port of the reaction tube, wherein the end of the sleeve 22 close to the pressure head 21 has a tube skirt 221 that is sleeved on the outside of the ring 24 and is used to squeeze the arc-shaped clamping jaws 241 to retract inward.

[0033] When in use, after covering the entire sealing film on the orifice plate at one time, the pressure cap 1 is pressed to move the pressure head 21 above the reaction tube close to the reaction tube. When the pressure head 21 approaches the reaction tube, the first spring 23 is compressed, and the reaction force presses the sealing film area on the port of the reaction tube. As the pressure head 21 approaches the reaction tube, the arc-shaped clamping claw 241 under the action of the spring 25 maintains an outward expansion shape, and the sealing film larger than the range of the reaction tube port is divided. As the pressure cap 1 is continuously pressed down, the pressure head 21 stops moving, so that the sleeve 22 continues to When the sleeve 22 approaches the reaction tube, it moves downward to squeeze the outer wall of the arc-shaped clamping jaw 241 until the arc-shaped clamping jaw 241 is completely retracted to form a ring 24, so that the edge of this part of the sealing film is covered on the outer wall of the reaction tube port to achieve rapid sealing. When the sleeve 22 moves away from the reaction tube, the first spring 23 releases energy to reset the pressure head 21, and the ring 24 also opens to facilitate the next sealing operation. Through the combination of the pressure cover 1 and the sealing mechanism 2, multiple reaction tubes in a 96-well plate can be sealed simultaneously, greatly improving the sealing efficiency.

[0034] Furthermore, a pressure pad 211 is fixedly connected to one end of the pressure head 21 away from the sleeve 22 ; when the pressure head 21 approaches the reaction tube, it first contacts the upper end of the reaction tube, thus playing a buffering and protective role.

[0035] In addition, a clamping pad 242 is fixedly connected to one side of the arc-shaped clamping jaw 241 close to the pressure head 21; when the arc-shaped clamping jaw 241 is retracted inwardly and wraps the reaction tube port, the clamping pad 242 first contacts the reaction tube port to play a role in buffering and enhancing friction.

[0036] Furthermore, the pressure head 21 is slidably connected to the sleeve 22; the sliding connection allows the pressure head 21 to remain stable and not rotate when moving on the sleeve 22, thereby preventing the sealing film from being misaligned due to stirring.

[0037] It should be added that the pressure cover 1 has a skirt 11 that engages with the orifice plate at the position corresponding to the edge of one side close to the orifice plate, and the height of the skirt 11 is less than the height of the orifice plate; when the pressure cover 1 is placed on the orifice plate, the skirt 11 will first contact and slightly engage with the edge of the orifice plate, providing a stable positioning reference for the pressure cover. At the same time, since the height of the skirt 11 is less than the height of the orifice plate, it can provide moving space for the pressure cover 1, thereby facilitating subsequent smooth operation.

[0038] Example 2

[0039] Different from Example 1, a rebound mechanism 3 is provided on the skirt 11, and the rebound mechanism 3 includes a telescopic leg 31 that moves closer to or away from the orifice plate on the skirt 11, a foot pad 32 fixedly connected to the end of the telescopic leg 31 away from the skirt 11, and a second spring 33 that is sleeved on the outside of the telescopic leg 31 and whose two ends are respectively connected to the skirt 11 and the foot pad 32, wherein the telescopic leg 31 is slidably connected to the skirt 11; when the pressure cover 1 is pressed down for sealing, the second spring 33 is squeezed by the foot pad 32 that contacts the bottom edge of the orifice plate, thereby releasing the pressed pressure cover 1 after the sealing is completed, so that the pressure cover 1 is squeezed back by the elastic force of the second spring 33, thereby separating the pressure cover 1 from the orifice plate for easy removal.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A 96-well plate rapid sealing device, characterized in that: It comprises a pressure cover (1) for covering the orifice plate and a sealing mechanism (2) arranged on the inner side of the pressure cover (1) at a position above the reaction tube corresponding to the orifice plate; The sealing mechanism (2) comprises a pressure head (21) which moves above the reaction tube and approaches or moves away from the reaction tube, a sleeve (22) which is sleeved on the outside of one end of the pressure head (21) away from the reaction tube and fixedly connected to the pressure cover (1), a first spring (23) which is sleeved on the outside of the pressure head (21) and whose two ends are respectively connected to the pressure head (21) and the sleeve (22), three arc-shaped clamping jaws (241) which are hingedly connected to the side of the pressure head (21) and distributed in a ring array, and a spring piece (25) which has one end fixedly connected to the side of the pressure head (21) and the other end in contact with the inner side of the arc-shaped clamping jaws (241), the three arc-shaped clamping jaws (241) forming a ring (24) sleeved on the end of the reaction tube, wherein the end of the sleeve (22) close to the pressure head (21) has a tube skirt (221) which is sleeved on the outside of the ring (24) and is used for pressing the arc-shaped clamping jaws (241) to be retracted inwardly.

2. A 96-well plate rapid sealing device according to claim 1, characterized in that: One end of the pressure head (21) away from the sleeve (22) is fixedly connected to a pressure pad (211).

3. A 96-well plate rapid sealing device according to claim 2, characterized in that: A clamping pad (242) is fixedly connected to a surface of the arc-shaped clamping jaw (241) close to the pressure head (21).

4. A 96-well plate rapid sealing device according to claim 3, characterized in that: The pressure head (21) is slidably connected to the sleeve (22).

5. A 96-well plate rapid sealing device according to claim 4, characterized in that: The pressure cover (1) has a skirt (11) at a position corresponding to the edge of one side of the orifice plate, which is engaged with the orifice plate. The height of the skirt (11) is smaller than the height of the orifice plate.

6. A 96-well plate rapid sealing device according to claim 5, characterized in that: The skirt (11) is provided with a rebound mechanism (3), the rebound mechanism (3) comprising a telescopic leg (31) that moves on the skirt (11) toward or away from the orifice plate, a foot pad (32) that is fixedly connected to an end of the telescopic leg (31) away from the skirt (11), and a foot pad (32) that is sleeved outside the telescopic leg (31) and A second spring (33) having two ends respectively connected to the skirt (11) and the foot pad (32), wherein: The telescopic legs (31) are slidably connected to the skirt (11).

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