Plate cover for deep hole plate
By designing the cover for deep-hole plates, fixing the pipette tip and reducing the equipment error rate, the equipment error problem caused by the inclination of the gun head in the deep-hole plate hole in the laboratory is solved, and the experimental efficiency and equipment life are improved.
Patent Information
- Application Number
- CN202421023520.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-13
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-05-13
AI Technical Summary
In the laboratory, the pipette tip of the deep-well plate is not fixed in the inclination direction of the hole, resulting in a high frequency of errors reported by the automated pipetting workstation equipment, affecting work efficiency and increasing maintenance costs.
Design a plate cover for deep-hole plates, including a cover body and baffle, and fix the pipette tip by moving the plate cover laterally to ensure that the pipette tip of the automated pipette device can enter the hole smoothly without poking it already The gun tip reduces the equipment error rate and reduces damage to the gun tip by the robotic arm through rounded corners and frame design.
It significantly reduces the error rate of the automated pipetting device, improves production efficiency, reduces operating costs, and extends the service life of the equipment.
Smart Images
Figure CN222348980U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of biological experiment technology, and in particular to a plate cover for a deep well plate. Background Art
[0002] In laboratory testing, deep-well plates are often used for various biological operations, which are very suitable for batch and automated operations. In the sample collection process, the tip of a pipette is generally used to dip into the bacterial sample, and then the tip with the bacterial sample is transferred to the deep-well plate for bacterial culture. After the bacterial culture is completed, the deep-well plate is transferred to the automated pipetting workstation for subsequent batch pipetting operations.
[0003] For example, in the colony screening process of genetic engineering plasmid construction, most laboratories use 10μl pipette tips to pick single clone colonies, and then transfer the 10μl pipette tips with single colonies to deep-well plates containing liquid culture medium for recovery culture. After the culture is completed, the positive clones are pipetted out for other experimental operations, such as plasmid extraction, preparation of glycerol bacteria, etc.
[0004] With the development of the current market and scientific research, the speed of experimental research and development is increasingly required. In order to meet the progress requirements of the market and scientific research, automated, high-throughput experimental processes are increasingly becoming the mainstream of contemporary laboratory development. However, we found in the actual production process that the tilt direction of the pipette tip for dipping the bacterial sample in the hole of the deep-well plate is not fixed and uniform. When the deep-well plate is directly placed on the automated pipetting workstation for batch pipetting, the pipette tip on the automated pipetting workstation often pokes the tip of the pipette dipped in the bacterial sample in the deep-well plate, resulting in a high frequency of equipment errors and the equipment cannot perform pipetting work normally. For this reason, it is also necessary to arrange special staff to guard the equipment and adjust it in time, which will greatly affect the work efficiency of the experimenters and increase operating costs; in addition, the pipetting device of the automated pipetting workstation is also prone to deformation, damage, etc., and the equipment maintenance cost is high.
[0005] Therefore, we urgently need an experimental equipment that can reduce the equipment error rate, avoid equipment damage, and improve production efficiency to solve the problems raised in the above background. Summary of the invention
[0006] In view of the defects existing in the prior art, the purpose of the present invention is to achieve the above purpose, and the technical solution adopted by the present invention is:
[0007] A plate cover for a deep well plate comprises a cover body, the upper surface of the cover body is provided with a plurality of through holes for adding liquid, and the cover body is surrounded by a first frame.
[0008] As a preferred solution of the present invention, a plurality of baffles are provided on the lower surface of the cover body, and the baffles are perpendicular to the lower surface of the cover body.
[0009] As a preferred solution of the utility model, the position and number of the baffles correspond to the position and number of the holes in the deep-well plate; the baffles are uniformly arranged on the same side of each through hole; the baffle is driven to move by moving the deep-well plate cover laterally, so that the first gun tip in the deep-well plate hole can be uniformly fixed on one side of the deep-well plate hole, which helps the second gun tip on the automated pipetting device to smoothly pass through the through hole and directly enter the deep-well plate hole to absorb liquid without poking the first gun tip already in the deep-well plate, thereby greatly reducing the error frequency of the automated pipetting device.
[0010] In the present application, the first gun tip is: a pipette tip used to transfer bacteria to a deep-well plate for culture; the second gun tip is: a pipette tip used on an automated pipetting device to transfer liquid in a deep-well plate.
[0011] As a preferred solution of the utility model, the inner surface of the first frame is also fixedly connected to a second frame; the height of the second frame is 1 to 4 mm lower than the height of the first frame. In the utility model, only the second frame of the deep-well plate cover is in direct contact with the upper surface of the deep-well plate, and the lower surface of the cover body is not in direct contact with the upper surface of the deep-well plate. This design can reduce the contact area between the lower surface of the cover body and the upper surface of the deep-well plate, reduce the friction between the two, and make the subsequent position offset of the deep-well plate cover smoother.
[0012] As a preferred solution of the utility model, the length and width of the second frame match the length and width of the upper surface of the deep well plate; the length and width of the first frame are respectively 1 to 3 mm larger than the length and width of the upper surface of the deep well plate.
[0013] The purpose of the first frame size being 1 to 3 mm larger than the length and width of the upper surface of the deep-well plate is that during long-term use, the accuracy of the robot arm in capturing the position of the hole of the deep-well plate will decrease. When the second gun tip happens to be stuck at the edge of the through hole, because the second gun tip is a conical structure with a wide upper side and a thin lower side, when the second gun tip gradually descends, the through hole will be slightly offset under the squeezing of the second gun tip, so that the second gun tip can smoothly pass through the through hole into the hole of the deep-well plate to absorb liquid; the first frame of the utility model provides space for the position offset of the through hole. Compared with the deep-well plate cover that can just get stuck on the upper surface of the deep-well plate, this design causes less damage to the second gun tip, and the error rate of the automated pipetting device is greatly reduced.
[0014] As a preferred solution of the utility model, the height of the baffle is 1 / 3 to 1 / 2 of the height of the hole of the deep-well plate. This height is reasonably designed, which can effectively fix the first gun tip and avoid the influence of the baffle on the bacterial solution in the deep-well plate due to the excessive length of the baffle.
[0015] As a preferred solution of the utility model, the edge of the through hole is rounded. When the second gun head happens to hit the edge of the through hole, the rounded corner can buffer the second gun head, help the second gun head to slide smoothly into the through hole, reduce the damage caused by direct contact between the second gun head and the cover body, and prevent the second gun head from being deformed and damaged.
[0016] As a preferred solution of the utility model, a non-slip groove 4 is provided on the side of the first frame to facilitate the staff to take the deep well plate cover.
[0017] Compared with the prior art, the advantages of the present invention are: the deep well plate cover of the present invention has a simple structure and a reasonable design. In actual use, the present invention can significantly reduce the error rate of the automated liquid transfer device, improve the accuracy of the automated liquid transfer device when absorbing the solution, greatly reduce the operating cost, improve the production efficiency, and have a very broad application prospect. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0019] Figure 1 This is a schematic diagram of the main structure of the deep hole plate cover plate of the utility model;
[0020] Figure 2 for Figure 1 Schematic diagram from top view;
[0021] Figure 3 for Figure 1 A schematic diagram of the main view;
[0022] Figure 4 It is a schematic diagram of the structure of the utility model when it is actually used;
[0023] Figure 5 for Figure 4 Schematic cross-sectional view of .
[0024] In the figure: 1. cover body; 2. through hole; 3. baffle; 4. anti-slip groove; 5. first frame; 51. second frame; 6. deep well plate; 7. automated pipetting device; 71. second gun tip; 8. first gun tip. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.
[0026] The embodiments of the present invention are further described in detail below with reference to the accompanying drawings.
[0027] like Figure 1 The plate cover for a deep well plate shown in the figure comprises a cover body 1 , the upper surface of which is provided with a plurality of through holes 2 for adding liquid, and the cover body 1 is surrounded by a first frame 5 .
[0028] Furthermore, a plurality of baffles 3 are provided on the lower surface of the cover body 1 , and the baffles 3 are perpendicular to the lower surface of the cover body 1 .
[0029] Furthermore, the position and number of the baffles 3 correspond to the position and number of the holes of the deep-well plate 6; the baffles 3 are uniformly arranged on the same side of each through-hole 2. By moving the cover of the deep-well plate 6 laterally to drive the baffle 3 to move, the first gun tip 8 in the hole of the deep-well plate 6 can be uniformly fixed on one side of the hole of the deep-well plate 6, which helps the second gun tip 71 on the automated liquid transfer device to smoothly pass through the through-hole 2 and directly enter the hole of the deep-well plate 6 to absorb liquid without poking the first gun tip 8 already in the deep-well plate 6, greatly reducing the error frequency of the automated liquid transfer device.
[0030] In the present application, the first gun tip is: a pipette tip used to transfer bacteria to a deep-well plate for culture; the second gun tip is: a pipette tip used on an automated pipetting device to transfer liquid in a deep-well plate.
[0031] like Figure 2 As shown, the inner surface of the first frame 5 is also fixedly connected to the second frame 51; the height of the second frame 51 is 1 to 4 mm lower than the height of the first frame 5. In the deep-well plate cover described in the present application, only the second frame 51 is in direct contact with the upper surface of the deep-well plate 6, and the lower surface of the cover body 1 is not in direct contact with the upper surface of the deep-well plate 6. This design can reduce the contact area between the lower surface of the cover body 1 and the upper surface of the deep-well plate 6, reduce the friction between the two, and make the subsequent position offset of the deep-well plate cover smoother.
[0032] Furthermore, the length and width of the second frame 51 match the length and width of the upper surface of the deep-hole plate 6 ; the length and width of the first frame 5 are respectively larger than the length and width of the upper surface of the deep-hole plate 6 by 1 to 3 mm.
[0033] The purpose of the first frame 5 being 1 to 3 mm larger than the length and width of the upper surface of the deep-well plate is that during the long-term use of the automated pipetting device, the accuracy of its mechanical arm in capturing the position of the holes of the deep-well plate 6 will decrease. When the second gun tip 71 happens to be stuck at the edge of the through-hole 2, because the second gun tip 8 as a whole is a conical structure with a wide upper side and a thin lower side, when the second gun tip 8 gradually descends, the through-hole 2 will be slightly shifted in position under the squeezing of the second gun tip 8, so that the second gun tip 8 can smoothly pass through the through-hole 2 and enter the hole of the deep-well plate 6 to absorb liquid; the first frame 5 of the present application provides space for the position shift of the through-hole 2; compared with the deep-well plate cover that can just catch the upper surface of the deep-well plate 6, this design causes less damage to the second gun tip 8, and the error rate of the automated pipetting device is greatly reduced.
[0034] like Figure 5 As shown, the height of the baffle plate 3 is 1 / 3 to 1 / 2 of the height of the hole of the deep-well plate 6. This height is reasonably designed, which can effectively fix the first gun head 8 and avoid the influence of the bacterial solution in the deep-well plate 6 caused by the excessive length of the baffle plate 3.
[0035] like Figure 1 As shown, the edge of the through hole 2 is designed with rounded corners. When the second gun head 71 happens to hit the edge of the through hole 2, the rounded corners can buffer the second gun head 71, helping the second gun head 71 to slide smoothly into the through hole 2, reducing the damage caused by direct contact between the second gun head 71 and the cover body 1, and preventing the second gun head 71 from being deformed and damaged.
[0036] Furthermore, a non-slip groove 4 is provided on the side of the first frame 5 to facilitate the staff to take the deep well plate cover.
[0037] The working principle of the utility model is:
[0038] Step 1: Use the first gun tip 8 to pick up a small amount of bacterial liquid and place it in the deep well plate 6 for culture; after the culture is completed, take the deep well plate cover described in the present application, gently insert each baffle 3 on the cover body 1 into one side of the corresponding hole on the deep well plate 6, and move the cover body 1 laterally from one side, and the moving distance is about 1 / 2-2 / 3 of the lateral distance of the aperture.
[0039] It is worth noting that in this process: the deep-well plate cover described in the present application does not need to be completely placed on the upper surface of the deep-well plate 6. The deep-well plate cover is lifted until the baffle 3 can contact the upper part of the first gun tip 8, and the deep-well plate cover is moved laterally so that the baffle 3 can block all the first gun tips 8 on one side of the deep-well plate 6 holes.
[0040] Step 2: After the first gun tip 8 is blocked on one side of the deep-well plate 6 hole, the deep-well plate cover is completely placed on the upper surface of the deep-well plate 6, and the deep-well plate 6 is transferred to the operating table of the automated pipetting device 7. The equipment parameters of the automated pipetting device 7 are adjusted so that the second gun tip 71 is located directly above the deep-well plate 6.
[0041] Step 3: Start the automated pipetting device 7, and make the second pipette head 71 descend and pass through the corresponding through hole 2 on the upper surface of the deep-well plate cover; wait until the second pipette head 71 absorbs the bacterial solution in the holes of the deep-well plate 6, then rise and transfer the bacterial solution, and the experiment is completed.
[0042] It should be noted that, in the present utility model, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.
[0043] The above description is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novel features applied for herein.
Claims
1. A plate cover for a deep well plate, characterized in that: The invention comprises a cover plate body (1), wherein the upper surface of the cover plate body (1) is provided with a plurality of through holes (2) for adding liquid, the cover plate body (1) is surrounded by a first frame (5), and the lower surface of the cover plate body (1) is provided with a plurality of baffles (3), wherein the baffles (3) are perpendicular to the lower surface of the cover plate body (1), the positions and the number of the baffles (3) correspond to the positions and the number of the holes of the deep hole plate, and the baffles (3) are uniformly arranged on the same side of each through hole (2).
2. A plate cover for a deep well plate as claimed in claim 1, characterized in that: The inner surface of the first frame (5) is also fixedly connected to a second frame (51); the height of the second frame (51) is 1 to 4 mm lower than the height of the first frame (5).
3. A plate cover for a deep well plate as claimed in claim 2, characterized in that: The length and width of the second frame (51) match the length and width of the upper surface of the deep-well plate; the length and width of the first frame (5) are respectively 1 to 3 mm larger than the length and width of the upper surface of the deep-well plate.
4. A plate cover for a deep well plate as claimed in claim 1, characterized in that: The height of the baffle (3) is 1 / 3 to 1 / 2 of the height of the hole of the deep hole plate.
5. A plate cover for a deep well plate as claimed in claim 1, characterized in that: The edge of the through hole (2) is designed with rounded corners.
6. A plate cover for a deep well plate as claimed in claim 1, characterized in that: An anti-slip groove (4) is provided on the side surface of the first frame (5).