Cell cryopreservation box
By designing a cell cryopreservation box using cross-linked polyethylene foam material and equipped with a cryopreservation carrier and a cooling chamber, the problems of high cost, frequent use of isopropyl alcohol and large space occupation of the existing cryopreservation boxes have been achieved. Improve cell survival rate and save space.
Patent Information
- Application Number
- CN202420890664.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-26
- Publication Date
- 2025-01-21
- Estimated Expiration
- 2034-04-26
AI Technical Summary
The existing program cooling and frozen storage box is expensive and requires regular replacement of the organic solvent isopropanol. The exposure of frozen cells to ambient temperature leads to a reduced survival rate, and the frozen storage box scattered space.
A cell cryopreservation box is designed, which is made of cross-linked polyethylene foam material and is equipped with a cryopreservation carrier and a polycold chamber. It can be sealed and stacked through the first and second socket devices to avoid the use of isopropyl alcohol and pass The card cavity quickly identifies cryopreserved cell information.
It reduces the frequency of use and replacement of isopropyl alcohol, improves cell survival rate, saves space in the ultra-low temperature refrigerator, and reduces the probability of the freezing box being forgotten.
Smart Images
Figure CN222373469U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell freezing and relates to a cell freezing box. Background Art
[0002] After freezing, cells need to go through a slow cooling process. They are usually frozen in a programmed cooling box. The cell cryopreservation tube is placed in the programmed cooling box, directly placed in a -80°C ultra-low temperature refrigerator overnight, and then transferred to a -196°C liquid nitrogen tank for long-term storage. However, the programmed cooling box is expensive and needs to be replaced regularly with the refrigerant isopropanol. As an organic solvent with certain biological toxicity, isopropanol is harmful to the human body and the environment. When multiple programmed cooling boxes are used to freeze multiple cells, they cannot be distinguished in appearance and need to be opened for identification, resulting in the frozen cells being exposed to ambient temperature, which can easily lead to problems such as reduced cell survival rate. When freezing a large number of cells, multiple programmed cooling boxes are scattered in the ultra-low temperature refrigerator, which can easily cause forgetting and take up more space in the ultra-low temperature refrigerator. In addition, when conducting cell monoclonal experiments, cell clones are usually stored in 96-well plates and placed in a -80°C ultra-low temperature refrigerator for long-term storage. Currently, the 96-well plates can only be simply wrapped and placed directly in a -80°C ultra-low temperature refrigerator, which makes it difficult to achieve the purpose of slow cooling, resulting in a decrease in cell survival rate. Utility Model Content
[0003] In order to solve the deficiencies in the prior art, the utility model aims to provide a cell freezing box.
[0004] The cell freezing box provided by the utility model comprises a cover body and a box body matched with the cover body, the outer sides of the cover body cooperate with the outer sides of the upper end of the box body to form a first sleeve device, the outer sides of the lower end of the box body are respectively provided with a second sleeve device, a freezing carrier is arranged inside the upper end of the box body, a cold collection cavity is arranged inside the lower end of the box body, a card cavity is arranged on a side surface of the box body perpendicular to the first sleeve device, and the bottom of the box body is connected to a box base.
[0005] Preferably, the cover body comprises an upper cover body and a cover body base, and the two are integrally formed; the box body base is integrally formed with the box body.
[0006] Preferably, the single first sleeve device comprises a first sleeve body arranged on one side of the upper cover body, a first locking buckle groove arranged on one side of the outer upper end of the box body, and a first locking buckle rotatably arranged on the first sleeve body; the first sleeve body and the first locking buckle groove are aligned in the vertical direction, and a rotatable first locking buckle is provided through the first sleeve body, and the first locking buckle forms a downward force on the cover body after being sleeved with the first locking buckle groove, and the cover body base is embedded in the box body, so that the cover body and the box body are sealed and fitted;
[0007] In a specific embodiment, the first sleeve body is a symmetrically arranged split structure, including two sleeve blocks with the same horizontal height, and the sleeve blocks are provided with a buckle that matches the diameter and shape of the rotating shaft of the first lock buckle. The length of the rotating shaft of the first lock buckle is slightly larger than the distance between the two sleeve blocks. The rotating shaft can be snapped into the buckle of the sleeve block to prevent the first lock buckle from slipping out of the first sleeve body; the length of the rotating shaft is 0.5 to 1.5 cm longer than the distance between the sleeve blocks, preferably 1 cm.
[0008] Preferably, a single second sleeve device includes a second sleeve body arranged on one side of the outer lower end of the box body, and a second lock buckle rotatably arranged on the second sleeve body; the second sleeve body is vertically aligned with the first sleeve body and the first lock buckle groove, and a rotatable second lock buckle is provided through the second sleeve body. When multiple boxes are stacked and used, the second sleeve device of the upper box body is used in conjunction with the first lock buckle groove of the lower box body, and the box body base of the upper box body is embedded in the box body of the lower freezing box, so that the multiple boxes can be stacked and fixed together;
[0009] In a specific embodiment, the second sleeve body is a symmetrically arranged split structure, including two sleeve blocks with the same horizontal height, and the sleeve blocks are provided with a buckle that matches the diameter and shape of the rotating shaft of the second lock buckle. The length of the rotating shaft of the second lock buckle is slightly larger than the distance between the two sleeve blocks. The rotating shaft can be snapped into the buckle of the sleeve block to prevent the second lock buckle from slipping out of the second sleeve body; the length of the rotating shaft is 0.5 to 1.5 cm longer than the distance between the sleeve blocks, preferably 1 cm.
[0010] Preferably, one or both sides of the cryopreservation carrier inside the upper end of the box body are provided with limit bars in a direction perpendicular to the first sleeve device, so as to facilitate the fit and fixation of the 96-well plate to be frozen to prevent it from shaking, and one or more limit bar notches are provided in the middle of the limit bar, so as to facilitate the operator to insert his fingers to take and place the fitted and fixed 96-well plate; the limit bar notches include U-shaped, semicircular, triangular or square, etc., which can be set according to actual needs.
[0011] Preferably, the area of the cryopreservation carrier other than the limiting strip, that is, the area on the cryopreservation carrier not covered by the limiting strip, is evenly provided with one or more cryopreservation cavities for storing cell cryopreservation tubes. After the cell cryopreservation tube is placed in the cryopreservation cavity, the top of the cryopreservation tube is higher than the top of the cryopreservation cavity, which is convenient for quick removal and placement of the cryopreservation tube. The operator can choose to freeze the 96-well plate or cell cryopreservation tube in the cryopreservation carrier according to actual conditions; the diameter of the cryopreservation cavity is 1.4 to 1.7 cm, preferably 1.5 cm, the depth is 2.8 to 3.5 cm, preferably 3 cm, and the distance between different cryopreservation cavities is 1.4 to 1.7 cm, preferably 1.5 cm.
[0012] Preferably, a cold-gathering cavity is provided inside the lower end of the box body, and the cold-gathering cavity is connected to the outside world through an air inlet. An air inlet is provided on the surface of the box body to facilitate the cold air in the ultra-low temperature refrigerator to enter the cold-gathering cavity. The cold air spontaneously enters the cold-gathering cavity through air convection. An adapted air inlet cap is provided at the air inlet to facilitate the adjustment of the inlet, exit and sealing of the cold air in the cold-gathering cavity. The cold-gathering cavity is a rectangular parallelepiped, with a length of 13 to 15 cm, preferably 14 cm, a width of 10 to 14 cm, preferably 12 cm, and a height of 1.5 to 3 cm, preferably 2 cm; the air inlet is circular, with a diameter of 1.5 to 3 cm, preferably 2 cm; the air inlet is cylindrical, with a diameter of 1 to 2.5 cm, preferably 1.5 cm, and a length of 1.5 to 3 cm, preferably 2 cm.
[0013] Preferably, a card cavity is provided on one side surface of the box body perpendicular to the first sleeve device, and a card slot is provided at the upper end of the card cavity. A recording paper containing information such as the name, quantity, and freezing date of the frozen cells can be inserted into the card cavity through the card slot, so as to facilitate rapid identification of the frozen cell information. If the recording paper is damaged during use, it should be replaced in time.
[0014] Preferably, the cover body can be used in conjunction with multiple boxes. When the multiple boxes are stacked, the second sleeve device of the upper box is used in conjunction with the first locking groove of the lower box, and the box base of the upper box is embedded in the box body of the lower freezing box, which can increase the sealing of the connection between the two boxes, achieve the purpose of slowly cooling the frozen cells in the freezing box, and improve the recovery efficiency of the frozen cells. After the multiple boxes are connected, the cover body can be connected to the most adjacent box through the first sleeve device, so that the cover body can be fixedly connected to the multiple boxes. The operator can flexibly select the number of boxes to be connected according to the actual number of frozen cells. When a large number of cells are frozen, the space in the ultra-low temperature refrigerator can be effectively saved, and the probability of a single freezing box being forgotten can be reduced.
[0015] Preferably, the cover, the cover base, the box, the freezing carrier and the box base are all made of cross-linked polyethylene foam material, so that the freezing box will not freeze your hands in a low temperature state, can be opened quickly, and can be quickly restored to room temperature after being taken out of the ultra-low temperature refrigerator, so that it is easy to reuse. The cross-linked polyethylene foam is a non-water-absorbent material and will not expand after absorbing water vapor, making the cover difficult to open.
[0016] Preferably, the first sleeve body, the first locking groove, and the second sleeve body are all made of high-density polycarbonate material, which has a certain hardness and can withstand low temperatures and repeated freezing and thawing.
[0017] Preferably, the first lock buckle, the second lock buckle and the air inlet cap are made of hard rubber, which has the characteristics of high hardness, wear resistance, corrosion resistance and certain ductility.
[0018] Preferably, the card cavity is made of transparent high-density polycarbonate material, so that the content marked on the recording paper in the card cavity can be clearly identified.
[0019] It can be seen from the technical solution provided by the utility model that the cell freezing box of the utility model has the following beneficial effects:
[0020] The utility model does not need to add organic solvents such as isopropyl alcohol into the box, thus avoiding the cumbersome operation of regularly replacing isopropyl alcohol and reducing the adverse effects of the use of isopropyl alcohol on the human body and the environment.
[0021] The cryopreservation carrier in the box of the utility model can freeze 96-well plates and cell cryopreservation tubes, and the functions can be switched according to experimental needs.
[0022] After the cells are placed in the utility model, the cover body is closed, the freezing box is sealed by the first sleeve devices on both sides, and the box is placed in an ultra-low temperature refrigerator. The air inlet cap of the air inlet is opened to guide the cold air in the ultra-low temperature refrigerator into the cold collection cavity, thereby helping to achieve the purpose of slowly cooling the cells.
[0023] When the utility model freezes cells, a recording paper containing information such as the name, quantity, and freezing date of the frozen cells can be inserted into the card cavity through the card insertion port, so as to facilitate rapid identification of the frozen cell information.
[0024] When the utility model takes out the cryopreservation box containing cells from the ultra-low temperature refrigerator for taking and placing operations, the air inlet cap is covered to seal the cold air in the cold-collecting cavity, so that the interior of the cryopreservation box is in a relatively low temperature state, slowing down the process of rapid temperature rise of the frozen cells when exposed to room temperature, and reducing the impact on the cell survival rate.
[0025] When a large number of cells are frozen, the utility model can stack and use a plurality of boxes. The box base of the upper box is embedded in the box body of the lower freezing box through the second sleeve device of the upper box and the first locking groove of the lower box, so that the plurality of boxes can be connected. After the cover body is connected to the most adjacent box through the first sleeve device, the cover body can be fixedly connected to the plurality of boxes, which can effectively save space in the ultra-low temperature refrigerator and reduce the probability of a single freezing box being forgotten. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For those skilled in the art, other drawings can be obtained based on these drawings without paying creative work.
[0027] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the utility model.
[0028] Figure 2 It is a front view of the overall structure of an embodiment of the utility model.
[0029] Figure 3 It is a side view of the overall structure of an embodiment of the utility model.
[0030] Figure 4 It is a schematic diagram of the internal structure of the box body of the utility model embodiment.
[0031] Figure 5 It is a top view of the internal structure of the box body of the embodiment of the utility model.
[0032] Figure 6 This is a cross-sectional view of the internal structure of the box body of the utility model embodiment.
[0033] Figure 7 It is a schematic diagram of the structure when multiple freezing boxes are stacked in an embodiment of the utility model.
[0034] Figure 1-Figure 7 In the figure, 1-cover body; 101-upper cover body; 102-cover body base; 2-box body; 3-first sleeve device; 301-first sleeve body; 302-first lock buckle; 303-first lock buckle groove; 4-second sleeve device; 401-second sleeve body; 402-second lock buckle; 5-cryostorage carrier; 501-cryostorage cavity; 502-limiting bar; 503-limiting bar notch; 6-card cavity; 7-card slot; 8-cold collecting cavity; 9-air inlet; 10-air inlet duct; 11-air inlet cap; 12-box body base. DETAILED DESCRIPTION
[0035] The utility model is further described in detail with reference to the following specific embodiments and drawings. The process, conditions, experimental methods, etc. for implementing the utility model, except for the contents specifically mentioned below, are all common knowledge and common common sense in the field, and the utility model has no special restrictions.
[0036] In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model is further described in detail below through the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the scope of the utility model. In addition, in the following description, the description of the known structure and technology is omitted to avoid unnecessary confusion of the concept of the utility model.
[0037] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.
[0038] In the description of the present application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.
[0039] The disclosure below provides many different embodiments or examples to realize the different structures of the present application. In order to simplify the disclosure of the present application, the parts and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the present application. In addition, the present application can repeat reference numbers and / or reference letters in different examples, and this repetition is for the purpose of simplification and clarity, which itself does not indicate the relationship between the various embodiments and / or settings discussed. In addition, the various specific processes and material examples provided by the present application, but those of ordinary skill in the art can appreciate the application of other processes and / or the use of other materials.
[0040] The utility model provides a cell freezing box, comprising a cover body 1 and a box body 2 matched with the cover body 1. The two sides of the outside of the cover body 1 cooperate with the two sides of the outside of the upper end of the box body 2 to form a first sleeve device 3, which is used to make the cover body 1 and the box body 2 sealed, fitted and fixed. The two sides of the outside of the lower end of the box body 2 are respectively provided with a second sleeve device 4. The upper end of the box body 2 is provided with a freezing carrier 5, which can be used for freezing a 96-well plate or a cell freezing tube. The lower end of the box body 2 is provided with a cold-collecting cavity 8, which is used in conjunction with an air inlet 9, an air inlet duct 10 and an air inlet cap 11 to adjust the inlet and outlet and sealing of the cold air in the cold-collecting cavity 8. A card cavity 6 is provided on a side surface of the box body 2 perpendicular to the first sleeve device 3, into which a recording paper containing frozen cell information can be inserted, so as to facilitate rapid identification of the frozen cell information. When freezing a large number of cells, the second sleeve devices 4 of multiple boxes can be used in conjunction with the first locking groove 303 to stack and use multiple freezing boxes, effectively saving space in an ultra-low temperature refrigerator and reducing the probability of a single freezing box being forgotten.
[0041] Specifically, see Figure 1-Figure 7 The utility model embodiment provides a cell freezing box, including a cover body 1 and a box body 2 matched with the cover body 1, the cover body 1 and the box body 2 are respectively provided with first sleeve devices 3 on both sides of the outer upper end, the box body 2 is respectively provided with second sleeve devices 4 on both sides of the outer lower end, a freezing carrier 5 is arranged inside the upper end of the box body 2, a cold collection cavity 8 is arranged inside the lower end of the box body 2, a card cavity 6 is arranged on the side surface of the box body 2 perpendicular to the first sleeve device 3, and the bottom of the box body 2 is connected to a box base 12.
[0042] The cover body 1 includes an upper cover body 101 and a cover body base 102, which are integrally formed.
[0043] A single first sleeve device 3 includes a first sleeve body 301 arranged on one side of the upper cover body 1, and a first locking groove 303 arranged on the outer side of the upper end of the box body 2. The first sleeve body 301 and the first locking groove 303 are aligned in the vertical direction. A rotatable first lock 302 is provided through the first sleeve body 301. After the first lock 302 is sleeved with the first locking groove 303, a downward force is applied to the cover body 1. The cover base 102 of the cover body 1 is embedded in the box body 2, so that the cover body 1 and the box body 2 are sealed and fit together.
[0044] A single second sleeve device 4 includes a second sleeve body 401 arranged on one side of the outer lower end of the box body 2, the second sleeve body 401 is aligned with the first sleeve body 301 and the first locking groove 303 in the vertical direction, and a rotatable second lock 402 is provided through the second sleeve body 401. When multiple boxes 2 are stacked and used, the second sleeve device 4 of the upper box body 2 is used in conjunction with the first locking groove 303 of the lower box body 2, and the box base 12 of the upper box body 2 is embedded in the box body 2 of the lower freezing box, so that multiple boxes 2 can be stacked and fixed together.
[0045] Limiting bars 502 are respectively provided on both sides of the cryopreservation carrier 5 inside the upper end of the box body 2 in a direction perpendicular to the first sleeve device 3, so as to facilitate the fitting and fixing of the 96-well plate to be frozen to prevent it from shaking. A limiting bar notch 503 is provided in the middle of the limiting bar 502 to facilitate the operator to insert his fingers to take and place the fitted and fixed 96-well plate.
[0046] The cryopreservation carrier 5 is evenly provided with a plurality of cryopreservation cavities 501 in the area other than the limiting strip 502, which are used to store cell cryopreservation tubes. After the cell cryopreservation tubes are placed in the cryopreservation cavities 501, the tops of the cryopreservation tubes are higher than the tops of the cryopreservation cavities 501, which facilitates the quick removal and placement of the cryopreservation tubes. The operator can choose to freeze the 96-well plate or the cell cryopreservation tubes in the cryopreservation carrier 5 according to the actual situation.
[0047] A cold collecting cavity 8 is provided inside the lower end of the box body 2, and the cold collecting cavity 8 is connected to the outside through an air inlet 10. An air inlet 9 is provided at the connection between the surface of the box body 2 and the internal cold collecting cavity 8, so that the cold air in the ultra-low temperature refrigerator can enter the cold collecting cavity 8. An adaptive air inlet cap 11 is provided at the air inlet 9, so as to adjust the inlet, outlet and sealing of the cold air in the cold collecting cavity 8.
[0048] A card cavity 6 is provided on one side surface of the box body 2 which is perpendicular to the first sleeve device 3. A card slot 7 is provided at the upper end of the card cavity 6. A recording paper containing information such as the name, quantity, and freezing date of the frozen cells can be inserted into the card cavity 6 through the card slot 7 to facilitate rapid identification of the frozen cell information.
[0049] The cover body 1 can be used in combination with multiple boxes 2. When multiple boxes 2 are stacked, the second sleeve device 4 of the upper box 2 is used in conjunction with the first locking groove 303 of the lower box 1. The box base 12 of the upper box 2 is embedded in the box 2 of the lower freezing box, so that multiple boxes 2 can be connected. After the cover body 1 is connected to the most adjacent box 2 through the first sleeve device 3, the cover body 1 can be fixedly connected to the multiple boxes 2. The operator can flexibly select the number of boxes to be connected according to the actual number of frozen cells. When a large number of cells are frozen, the space in the ultra-low temperature refrigerator can be effectively saved, and the probability of a single freezing box being forgotten can be reduced.
[0050] The cover 1, the cover base 102, the box 2, the freezing carrier 5, and the box base 12 are all made of cross-linked polyethylene foam material, so that the freezing box will not freeze your hands in a low temperature state, can be opened quickly, and can be quickly restored to room temperature after being taken out of the ultra-low temperature refrigerator, which is convenient for repeated use. The cross-linked polyethylene foam is a non-water-absorbent material and will not expand after absorbing water vapor, making it difficult to open the cover.
[0051] The first sleeve body 301, the first locking groove 303, and the second sleeve body 401 are all made of high-density polycarbonate material, which has a certain hardness and can withstand low temperatures and repeated freezing and thawing.
[0052] The first lock buckle 302, the second lock buckle 402, and the air inlet cap 11 are made of hard rubber, which has the characteristics of high hardness, wear resistance, corrosion resistance and certain ductility.
[0053] The card cavity 6 is made of transparent high-density polycarbonate material, so that the content marked on the recording paper in the card cavity 6 can be clearly identified.
[0054] Working process of this utility model:
[0055] The operator places the cell cryopreservation tube or 96-well plate to be frozen in the cryopreservation carrier 5, and can switch the cryopreservation purpose of the cryopreservation carrier 5 between the 96-well plate and the cell cryopreservation tube according to actual conditions.
[0056] When freezing a 96-well plate, after sealing the gap at the cover of the 96-well plate with a sealing film, place the 96-well plate in the freezing carrier 5 with your fingers through the limit bar notch 503 of the limit bar 502 in the freezing carrier 5, the limit bar 502 fits and fixes the 96-well plate to prevent it from shaking, cover the cover 1, and the cover base 102 is embedded in the box 2. The freezing box is sealed by the first sleeve device 3 on both sides of the upper cover 101 and the box 2, and the recording paper containing the frozen cell information is inserted into the card cavity 6 through the card slot 7, and the air inlet cap 11 at the air inlet 9 is opened to allow the cold air in the ultra-low temperature refrigerator to pass through the air inlet 10. When the 96-well plate needs to be taken out, the air inlet cap 11 at the air inlet 9 is plugged to seal the cold air in the cold chamber 8, so that the 96-well plate can still be kept at a relatively low temperature after being taken out of the ultra-low temperature refrigerator. After taking the cryopreservation box out of the ultra-low temperature refrigerator, the first sleeve device 3 is released, the cover body 1 is opened, and the operator takes out the 96-well plate that is placed in a close fit through the limit bar notch 503 provided in the middle of the limit bar 502, opens the air inlet cap 11 at the air inlet 9, releases the cold air in the cold chamber 8, accelerates the temperature rise of the cryopreservation box, and facilitates reuse.
[0057] When freezing a cell cryopreservation tube, the cell cryopreservation tube is placed in the cryopreservation cavity 501 in the cryopreservation carrier 5, the cover 1 is covered, the cover base 102 is embedded in the box 2, the cryopreservation box is sealed by the upper cover 101 and the first sleeve devices 3 on both sides of the box 2, the recording paper containing the cryopreservation cell information is inserted into the card cavity 6 through the card slot 7, the air inlet cap 11 at the air inlet 9 is opened, so that the cold air in the ultra-low temperature refrigerator can spontaneously enter the cold chamber 8 through the air inlet 10, and the cryopreservation box is placed in the freezer. Place it in an ultra-low temperature refrigerator for cooling; when it is necessary to take out the cell cryopreservation tube, plug the air inlet cap 11 at the air inlet 9 to seal the cold air in the cold collection chamber 8, so that the cell cryopreservation tube can still be kept at a relatively low temperature after being taken out of the ultra-low temperature refrigerator. After taking the freezing box out of the ultra-low temperature refrigerator, release the first sleeve device 3, open the cover 1, take out the cell cryopreservation tube, open the air inlet cap 11 at the air inlet 9, release the cold air in the cold collection chamber 8, accelerate the temperature rise of the freezing box, and facilitate reuse.
[0058] The operator can choose to stack different numbers of freezing boxes for use according to the actual number of frozen cells. When multiple boxes are stacked, the second sleeve device 4 of the upper box and the first locking groove 303 of the lower box are used in conjunction with each other, and the box base 12 of the upper box 2 is embedded in the box 2 of the lower freezing box, so that multiple boxes 2 can be connected. After the cover 1 is connected to the most adjacent box 2 through the first sleeve device 3, the cover 1 can be fixedly connected to the multiple boxes 2. When a large number of cells are frozen, the space in the ultra-low temperature refrigerator can be effectively saved, and the probability of a single freezing box being forgotten can be reduced.
[0059] Example 1
[0060] In this embodiment, a cover body 1 is matched with a box body 2, and the cover body 1 and the box body 2 are fixedly connected via a first sleeve device 3. The first lock buckle 302 of the first sleeve device 3 is rotatably arranged on the first sleeve body 301. The first sleeve body 301 includes two sleeve blocks with the same horizontal height. The length of the rotating axis of the first lock buckle 302 is 1 cm longer than the distance between the two sleeve blocks. The cover body 1 and the box body 2 are fixed by locking the first lock buckle 302 in the first lock buckle groove 303.
[0061] In this embodiment, a limiting bar 502 is provided on the cryopreservation carrier 5 inside the box 2 for fixing the 96-well plate, and a U-shaped limiting bar notch 503 is provided in the middle of the limiting bar to facilitate the placement and removal of the 96-well plate.
[0062] In the present embodiment, when freezing a 96-well plate, after sealing the gap at the cover of the 96-well plate with a sealing film, the 96-well plate is placed in the freezing carrier 5 by a finger through the U-shaped limit bar notch 503 of the limit bar 502 in the freezing carrier 5, and the limit bar 502 fits and fixes the 96-well plate to prevent it from shaking. The cover 1 is closed, and the cover base 102 is embedded in the box 2. The freezing box is sealed by the first sleeve device 3 on both sides of the upper cover 101 and the box 2. The recording paper containing the frozen cell information is inserted into the card cavity 6 through the card slot 7, and the air inlet cap 11 at the air inlet 9 is opened to allow the cold air in the ultra-low temperature refrigerator to pass through the air inlet duct. 10 can spontaneously enter the cold-gathering chamber 8, and place the cryopreservation box in the ultra-low temperature refrigerator for cooling; when the 96-well plate needs to be taken out, the air inlet cap 11 at the air inlet 9 is plugged to seal the cold air in the cold-gathering chamber 8, so that the 96-well plate can still be kept at a relatively low temperature after being taken out of the ultra-low temperature refrigerator. After taking the cryopreservation box out of the ultra-low temperature refrigerator, the first sleeve device 3 is released, the cover body 1 is opened, and the operator takes out the 96-well plate that is placed in a close fit through the U-shaped limit strip notch 503 provided in the middle of the limit strip 502, opens the air inlet cap 11 at the air inlet 9, releases the cold air in the cold-gathering chamber 8, accelerates the temperature rise of the cryopreservation box, and facilitates reuse.
[0063] Example 2
[0064] In this embodiment, one cover body 1 cooperates with two boxes 2 , the cover body 1 and the uppermost box body 2 are fixedly connected via a first sleeve device 3 , and the boxes 2 are fixedly connected via a second sleeve device 4 and the first locking groove 303 .
[0065] The first lock buckle 302 of the first sleeve device 3 is rotatably arranged on the first sleeve body 301, and the first sleeve body 301 includes two sleeve blocks with the same horizontal height. The length of the rotation axis of the first lock buckle 302 is 1 cm longer than the distance between the two sleeve blocks. By locking and fixing the first lock buckle 302 in the first lock buckle groove 303, the cover body 1 is fixed to the topmost box body 2.
[0066] A second sleeve device 4 is provided on one side of the outer lower side of the box body 2, and a second lock buckle 402 is rotatably provided on the second sleeve body 401 in the second sleeve device 4. The second sleeve body 401 includes two sleeve blocks with the same horizontal height. The length of the rotation axis of the second lock buckle 402 is 1 cm longer than the distance between the two sleeve blocks. The upper box body is fixed to the lower box body 2 by locking the second lock buckle 402 of the upper box body in the first lock buckle groove 303 of the lower box body.
[0067] In this embodiment, 12 cryopreservation cavities 501 are provided on the cryopreservation carrier 5 for storing cell cryopreservation tubes.
[0068] In the present embodiment, when freezing cell cryopreservation tubes, the cell cryopreservation tubes are placed in the freezing chamber 501 in the freezing carrier 5 of the two boxes 2, the cover 1 is covered, the cover base 102 is embedded in the box 2, the freezing box is sealed by the upper cover 101 and the first sleeve devices 3 on both sides of the box 2, the recording paper containing the frozen cell information is inserted into the card chamber 6 through the card slot 7, the air inlet cap 11 at the air inlet 9 is opened, so that the cold air in the ultra-low temperature refrigerator can spontaneously enter the cold chamber 8 through the air inlet 10, and the freezing box is placed in the ultra-low temperature refrigerator. The temperature is lowered in the low-temperature refrigerator; when it is necessary to take out the cell cryopreservation tube, the air inlet cap 11 at the air inlet 9 is plugged to seal the cold air in the cold-collecting cavity 8, so that the cell cryopreservation tube can still be kept at a relatively low temperature after being taken out of the ultra-low temperature refrigerator. After taking the freezing box out of the ultra-low temperature refrigerator, the first sleeve device 3 and the second sleeve device 4 are released, the cover body 1 is opened, the cell cryopreservation tubes in the two boxes 2 are taken out, and the air inlet caps 11 at the air inlet 9 of the two boxes are opened to release the cold air in the cold-collecting cavity 8, accelerate the temperature rise of the freezing box, and facilitate reuse.
[0069] Example 3
[0070] In this embodiment, one cover body 1 cooperates with two boxes 2 , the cover body 1 and the uppermost box body 2 are fixedly connected via a first sleeve device 3 , and the boxes 2 are fixedly connected via a second sleeve device 4 and the first locking groove 303 .
[0071] The first lock buckle 302 of the first sleeve device 3 is rotatably arranged on the first sleeve body 301, and the first sleeve body 301 includes two sleeve blocks with the same horizontal height. The length of the rotation axis of the first lock buckle 302 is 0.8 cm longer than the distance between the two sleeve blocks. By locking and fixing the first lock buckle 302 in the first lock buckle groove 303, the cover body 1 is fixed to the topmost box body 2.
[0072] A second sleeve device 4 is provided on one side of the lower outer side of the box body 2, and a second lock buckle 402 is rotatably provided on the second sleeve body 401 in the second sleeve device 4, and the second sleeve body 401 includes two sleeve blocks with the same horizontal height, and the length of the rotation axis of the second lock buckle 402 is 0.8 cm longer than the distance between the two sleeve blocks. The upper box body is fixed to the lower box body 2 by locking the second lock buckle 402 of the upper box body in the first lock buckle groove 303 of the lower box body.
[0073] In this embodiment, one of the freezing box boxes is used for freezing 96-well plates, and the other freezing box box is used for freezing cell cryopreservation tubes; in the box for freezing 96-well plates, a limiting bar 502 with a limited position is provided on the freezing carrier 5 inside the box 2 for fixing the 96-well plate, and a semicircular limiting bar notch 503 is provided in the middle of the limiting bar to facilitate the placement and removal of the 96-well plate; in the box for freezing cell cryopreservation tubes, 16 freezing cavities 501 are provided on the freezing carrier 5 for storing cell cryopreservation tubes;
[0074] In this embodiment, when freezing a 96-well plate, after sealing the gap at the cover of the 96-well plate with a sealing film, the 96-well plate is placed in the freezing carrier 5 through the semicircular limiting bar notch 503 of the limiting bar 502 in the freezing carrier 5, and the limiting bar 502 fits and fixes the 96-well plate to prevent it from shaking; when freezing a cell freezing tube, the cell freezing tube is placed in the freezing cavity 501 in the freezing carrier 5 of the box 2;
[0075] The cover 1 is closed, and the cover base 102 is embedded in the box 2. The cryopreservation box is sealed by the upper cover 101 and the first sleeve devices 3 on both sides of the box 2. The recording paper containing the cryopreserved cell information is inserted into the card cavity 6 through the card slot 7. The air inlet cap 11 at the air inlet 9 is opened to allow the cold air in the ultra-low temperature refrigerator to spontaneously enter the cold chamber 8 through the air inlet 10. The cryopreservation box is placed in the ultra-low temperature refrigerator for cooling. When the 96-well plate or cell cryopreservation tube needs to be taken out, the air inlet cap 11 at the air inlet 9 is plugged to seal the cold air in the cold chamber 8. After the freezing box is taken out of the ultra-low temperature refrigerator, the first sleeve device 3 and the second sleeve device 4 are released, and the cover body 1 is opened. The operator takes out the 96-well plate or the cell freezing tube from the freezing chamber 501 through the semicircular limit bar notch 503 provided in the middle of the limit bar 502, and opens the air inlet caps 11 at the air inlets 9 of the two boxes to release the cold air in the cold chamber 8, accelerate the temperature rise of the freezing box, and facilitate reuse.
[0076] The protection content of the present invention is not limited to the above embodiments. Without departing from the spirit and scope of the utility model concept, changes and advantages that can be thought of by those skilled in the art are included in the present invention and are protected by the attached claims.
Claims
1. A cell freezing box, characterized in that: include: A cover body (1) and a box body (2) matched with the cover body (1), the two sides of the outside of the cover body (1) cooperate with the two sides of the outside of the upper end of the box body (2) to form a first sleeve device (3), the two sides of the outside of the lower end of the box body (2) are respectively provided with a second sleeve device (4), the upper end of the box body (2) is provided with a freezing carrier (5), the lower end of the box body (2) is provided with a cold storage cavity (8), the side surface of the box body (2) perpendicular to the first sleeve device (3) is provided with a card cavity (6), and the bottom of the box body (2) is connected to a box base (12).
2. The cell freezing box according to claim 1, characterized in that: The cover body (1) comprises an upper cover body (101) and a cover body base (102), both of which are integrally formed; the box body base (12) is integrally formed with the box body (2).
3. The cell freezing box according to claim 2, characterized in that: The single first sleeve device (3) comprises a first sleeve body (301), a first lock buckle (302), and a first lock buckle groove (303); the first sleeve body (301) is arranged on the outer side of the upper cover body (101), the first lock buckle groove (303) is arranged on the outer side of the upper end of the box body (2), the first sleeve body (301) and the first lock buckle groove (303) are aligned in the vertical direction, and the first lock buckle (302) is rotatably arranged on the first sleeve body (301).
4. The cell freezing box according to claim 3, characterized in that: The first sleeve body (301) is a symmetrically arranged split structure, comprising two sleeve blocks with the same horizontal height; the sleeve blocks are provided with buckles that match the diameter and shape of the rotating shaft of the first lock buckle (302); the length of the rotating shaft of the first lock buckle (302) is 0.5 to 1.5 cm longer than the distance between the two sleeve blocks, and the rotating shaft is snapped into the buckle of the sleeve block.
5. The cell freezing box according to claim 3, characterized in that: A single second sleeve device (4) comprises a second sleeve body (401) and a second lock buckle (402); the second sleeve body (401) is arranged on one side of the outer lower end of the box body (2); the second sleeve body (401) is vertically aligned with the first sleeve body (301) and the first lock buckle groove (303); and the second lock buckle (402) is rotatably arranged on the second sleeve body (401).
6. The cell freezing box according to claim 5, characterized in that: The second sleeve body (401) is a symmetrically arranged split structure, comprising two sleeve blocks with the same horizontal height, and the sleeve blocks are provided with a buckle that matches the diameter and shape of the rotating shaft of the second lock buckle (402). The length of the rotating shaft of the second lock buckle (402) is 0.5 to 1.5 cm longer than the distance between the two sleeve blocks, and the rotating shaft is snapped into the buckle of the sleeve block.
7. The cell freezing box according to claim 1, characterized in that: A limiting strip (502) is provided on one or both sides of the freezing carrier (5) inside the upper end of the box body (2) in a direction perpendicular to the first sleeve device (3), and a limiting strip notch (503) is provided in the middle of the limiting strip (502); The notch of the limiting strip includes a U-shape, a semicircle, a triangle or a square.
8. The cell freezing box according to claim 1, characterized in that: The freezing carrier (5) is evenly provided with one or more freezing cavities (501) in the area other than the limiting strip (502); the diameter of the freezing cavity is 1.4 to 1.7 cm, and the depth is 2.8 to 3.5 cm; the distance between different freezing cavities is 1.4 to 1.7 cm.
9. The cell freezing box according to claim 1, characterized in that: The cooling chamber (8) is connected to the outside world via an air inlet (10); an air inlet (9) is provided on the surface of the box body (2); and an adapted air inlet cap (11) is provided at the air inlet (9); The length of the cooling chamber (8) is 13 to 15 cm, the width is 10 to 14 cm, and the height is 1.5 to 3 cm; the air inlet (9) is circular, with a diameter of 1.5 to 3 cm; the air inlet duct (10) is cylindrical, with a diameter of 1 to 2.5 cm, and a length of 1.5 to 3 cm.
10. The cell freezing box according to claim 1, characterized in that: A card cavity (6) is provided on a side surface of the box body (2) perpendicular to the first sleeve device (3), and a card insertion port (7) is provided at the upper end of the card cavity (6).