Small movable liquid sterile filtering device

Abstract

The utility model provides a small movable liquid sterile filtering device, and belongs to the field of fermentation engineering. The device comprises a culture medium component storage tank, a liquid filter and a movable cart, the culture medium component storage tank and the liquid filter are both arranged on the movable cart, an air inlet is formed in the culture medium component storage tank, a first pipeline is connected to the air inlet of the culture medium component storage tank, and a second pipeline is connected to the liquid filter. The outlet end of the culture medium component storage tank is connected with the inlet end of the liquid filter through a second pipeline. The culture medium can be immediately used after being connected according to actual needs, can also meet the requirement for sterile filtration treatment of growth factor nutrient substances which are not resistant to high temperature in the culture medium, and is mainly used for sterile filtration treatment of part of components of the culture medium.

Description

Technical Field

[0001] The utility model belongs to the field of fermentation engineering, in particular to a small movable liquid aseptic filtering device. Background Art

[0002] At present, the culture medium sterilization method of microbial fermentation production enterprises is usually continuous sterilization. With the improvement and upgrading of technology, instantaneous high-temperature continuous sterilization is more and more widely used. Microbial culture medium contains certain growth factor nutrients, which are sensitive to high temperature conditions. Although instantaneous high-temperature continuous sterilization reduces the loss of nutrients compared with ordinary continuous sterilization, it still has certain damage to some growth factor nutrients, resulting in the loss of nutrients. Therefore, it is considered to perform separate sterile filtration treatment on growth factors or other heat-inlaboratory nutrient components in the culture medium.

[0003] Since the amount of growth factor nutrients added is usually small, 1L-10L can meet the fermentation amount after being dissolved into liquid, so it is not suitable to add large and medium-sized liquid filtration equipment alone. In the actual production process, the culture medium of some fermentation products does not contain culture medium with heat-resistant substances, and there is no need to use a separate liquid filtration equipment to filter the heat-resistant substance components separately. Therefore, it is considered to design a small liquid filtration device that can be used as soon as it is connected according to actual needs and can meet the sterile filtration treatment of heat-resistant growth factor nutrients in the culture medium. Utility Model Content

[0004] In view of this, the utility model aims to provide a small movable liquid sterile filtration device to solve the above-mentioned problems.

[0005] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a small movable liquid aseptic filtration device, which includes a culture medium component storage tank, a liquid filter and a mobile cart, the culture medium component storage tank and the liquid filter are both arranged on the mobile cart, the culture medium component storage tank is provided with an air inlet, the air inlet of the culture medium component storage tank is connected to a first pipeline, and the outlet end of the culture medium component storage tank is connected to the inlet end of the liquid filter through a second pipeline.

[0006] Furthermore, the outlet end of the liquid filter is connected to a third pipeline.

[0007] Furthermore, a feed valve is provided on the third pipeline.

[0008] Furthermore, a pressure gauge is provided on the liquid filter, and the pressure gauge is connected to the liquid filter through a fourth pipeline.

[0009] Furthermore, the second pipeline is connected to the first exhaust pipeline, the fourth pipeline is connected to the second exhaust pipeline, and the third pipeline on both sides of the feed valve is connected to the third exhaust pipeline and the fourth exhaust pipeline respectively.

[0010] Furthermore, the first exhaust pipeline, the second exhaust pipeline, the third exhaust pipeline and the fourth exhaust pipeline are all provided with exhaust valves.

[0011] Furthermore, the culture medium component storage tank is provided with a material pouring inlet, and an inlet cover is provided at the material pouring inlet.

[0012] Furthermore, the volume of the culture medium component storage tank is 20L.

[0013] Furthermore, the size of the filter element in the liquid filter is 5 inches.

[0014] Furthermore, the size of the filter pores of the filter element in the liquid filter is 0.22 μm.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. The utility model provides a small movable liquid aseptic filtration device, which is connected to steam through a first pipeline to sterilize the device, and then connected to process air through the first pipeline to press the material in the culture medium component storage tank into the liquid filter for filtration and then output to the fermentation tank through the fourth pipeline, thereby realizing the separate aseptic filtration treatment of growth factor nutrients in the culture medium that are not resistant to high temperatures;

[0017] 2. The utility model provides a small movable liquid sterile filtration device, which realizes the mobility of the device by setting a mobile cart, and has flexible usage scenarios. The device has a simple structure, is plug-and-play, and is convenient and fast. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the accompanying drawings:

[0019] Figure 1 The utility model is a schematic diagram of the overall structure of a small movable liquid sterile filtration device.

[0020] 1-culture medium component storage tank, 2-liquid filter, 3-mobile cart, 4-pressure gauge, 5-first pipeline, 6-second pipeline, 7-third pipeline, 8-fourth pipeline, 9-feed valve, 10-first exhaust pipeline, 11-second exhaust pipeline, 12-third exhaust pipeline, 13-fourth exhaust pipeline, 14-dumping inlet. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely explain the technical solutions in the embodiments of the utility model. It should be noted that the embodiments and features in the embodiments of the utility model can be combined with each other without conflict, and the described embodiments are only part of the embodiments of the utility model, not all of the embodiments.

[0022] See also Figure 1 This embodiment describes a small movable liquid aseptic filtration device, which includes a culture medium component storage tank 1, a liquid filter 2 and a mobile cart 3. The culture medium component storage tank 1 and the liquid filter 2 are both arranged on the mobile cart 3. The culture medium component storage tank 1 is provided with an air inlet, and the air inlet of the culture medium component storage tank 1 is connected to a first pipeline 5. The outlet end of the culture medium component storage tank 1 is connected to the inlet end of the liquid filter 2 through a second pipeline 6. The culture medium component storage tank 1 in this embodiment is used to store sterile aqueous solutions of some high-temperature-intolerant substances such as growth factors and nutrients in the culture medium, and the liquid filter 2 is used to filter the sterile aqueous solution in the culture medium component storage tank 1. The first pipeline 5 described in this embodiment can be connected to steam or sterile process air according to actual needs. When When the device performs air sterilization operation, the first pipeline 5 is connected to steam for disinfection. When the material in the culture medium component storage tank 1 needs to be input into the liquid filter 2 for filtration, the first pipeline 5 is connected to the sterile process air, and the material in the culture medium component storage tank 1 is pressed into the liquid filter 2 through the second pipeline 6 for filtration. In this embodiment, the first pipeline 5 is connected to steam to perform air sterilization on the device, and then the first pipeline 5 is connected to the process air to press the material in the culture medium component storage tank 1 into the liquid filter 2 for filtration and then output to the fermentation tank, thereby achieving separate sterile filtration treatment of some high-temperature-sensitive substances such as growth factors and nutrients in the culture medium, and the mobile function of the device is realized by setting a mobile cart 3, and the usage scenario is flexible. The device described in this embodiment has a simple structure, is ready to use, and is convenient and fast.

[0023] In this embodiment, the outlet end of the liquid filter 2 is connected to the third pipeline 7, and the outlet end of the liquid filter 2 is connected to the fermentation tank through the third pipeline 7. The material filtered by the liquid filter 2 is input into the fermentation tank through the third pipeline 7.

[0024] In this embodiment, a feed valve 9 is provided on the third pipeline 7 , and the feed valve 9 is used to control the on-off of the third pipeline 7 .

[0025] In this embodiment, a pressure gauge 4 is provided on the liquid filter 2 . The pressure gauge 4 is connected to the liquid filter 2 via a fourth pipeline 8 . The pressure gauge 4 is used to measure the pressure in the liquid filter 2 .

[0026] In this embodiment, the second pipeline 6 is connected to the first exhaust pipeline 10, the fourth pipeline 8 is connected to the second exhaust pipeline 11, and the third pipeline 7 on both sides of the feed valve 9 are respectively connected to the third exhaust pipeline 12 and the fourth exhaust pipeline 13. The first exhaust pipeline 10, the second exhaust pipeline 11, the third exhaust pipeline 12 and the fourth exhaust pipeline 13 are used to exhaust the device when the device is sterilized by steam.

[0027] In this embodiment, exhaust valves are provided on the first exhaust pipeline 10 , the second exhaust pipeline 11 , the third exhaust pipeline 12 and the fourth exhaust pipeline 13 , and the exhaust valves are used to control the on-off of the exhaust pipelines.

[0028] In this embodiment, the culture medium component storage tank 1 is provided with a pouring inlet 14, and an inlet cover is provided at the pouring inlet 14. Some high-temperature-sensitive material components such as growth factors and nutrients in the culture medium are dissolved with sterile process water and then input into the culture medium component storage tank 1 through the pouring inlet 14. After pouring, the inlet cover is closed to ensure the sealing inside the culture medium component storage tank 1.

[0029] The volume of the culture medium component storage tank 1 described in this embodiment is 20L. Since the amount of growth factor-like nutrients added to the culture medium is usually small, 1L-10L of liquid dissolved in liquid can meet the fermentation dosage. Therefore, a culture medium component storage tank 1 with a volume of 20L is selected as the material storage container.

[0030] The liquid filter 2 in this embodiment is a small liquid filter. The size of the filter element in the liquid filter 2 is 5 inches, which can meet the filtering requirements of the culture medium components.

[0031] In this embodiment, the culture medium component storage tank 1 and the liquid filter 2 are both small-sized devices. On the basis of meeting the filtering requirements of the culture medium components, the usage scenarios are more flexible, and they are ready for use, convenient and fast.

[0032] The size of the filter pores in the filter element of the liquid filter 2 in this embodiment is 0.22 μm.

[0033] The working principle in this embodiment is as follows:

[0034] First, liquid culture medium is prepared in the laboratory, and some high-temperature-sensitive material components such as growth factors and nutrients in the culture medium are dissolved quantitatively with sterile process water for standby use. The third pipeline 7 is connected to the fermentation tank, and the first exhaust pipeline 10, the second exhaust pipeline 11, the third exhaust pipeline 12, the fourth exhaust pipeline 13 and the feed valve 9 are opened. The first pipeline 5 is connected to steam to sterilize the entire device and the fermentation tank. The steam is discharged from the first exhaust pipeline 10, the second exhaust pipeline 11, the third exhaust pipeline 12 and the fourth exhaust pipeline 13 respectively. During the sterilization process, the pressure of the pressure gauge 4 is maintained. At about 0.1kg, after air sterilization is completed, close the first exhaust pipeline 10, the second exhaust pipeline 11, the third exhaust pipeline 12 and the fourth exhaust pipeline 13, open the feed valve 9, input the material solution into the culture medium component storage tank 1 through the pouring inlet 14, and then close the inlet cover, connect the first pipeline 5 to the sterile process air, press the material in the culture medium component storage tank 1 into the liquid filter 2 through the second pipeline 6 for filtration, and then enter the fermentation tank through the third pipeline 7. After all the materials have entered the fermentation tank, disassemble the device, rinse it with water, and move it to the placement location through the mobile cart 3 for standby use.

[0035] The utility model has a simple structure, can be used immediately after being connected, does not occupy space, is detachable, and is convenient and quick.

[0036] The embodiments of the utility model disclosed above are only used to help explain the utility model. The embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. According to the content of this specification, many modifications and changes can be made. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model.

Claims

1. A small movable liquid sterile filtration device, characterized in that: The invention comprises a culture medium component storage tank (1), a liquid filter (2) and a mobile cart (3), wherein the culture medium component storage tank (1) and the liquid filter (2) are both arranged on the mobile cart (3), the culture medium component storage tank (1) is provided with an air inlet, the air inlet of the culture medium component storage tank (1) is connected to a first pipeline (5), the outlet end of the culture medium component storage tank (1) is connected to the inlet end of the liquid filter (2) via a second pipeline (6), and the outlet end of the liquid filter (2) is connected to a A third pipeline (7), wherein a feed valve (9) is arranged on the third pipeline (7), a pressure gauge (4) is arranged on the liquid filter (2), and the pressure gauge (4) is connected to the liquid filter (2) via a fourth pipeline (8), the second pipeline (6) is connected to a first exhaust pipeline (10), the fourth pipeline (8) is connected to a second exhaust pipeline (11), and the third pipeline (7) on both sides of the feed valve (9) is respectively connected to a third exhaust pipeline (12) and a fourth exhaust pipeline (13).

2. A small movable liquid sterile filtration device according to claim 1, characterized in that: The first exhaust pipeline (10), the second exhaust pipeline (11), the third exhaust pipeline (12) and the fourth exhaust pipeline (13) are all provided with exhaust valves.

3. A small movable liquid sterile filtration device according to claim 1, characterized in that: The culture medium component storage tank (1) is provided with a material pouring inlet (14), and the material pouring inlet (14) is provided with an inlet cover.

4. A small movable liquid sterile filtration device according to claim 1, characterized in that: The volume of the culture medium component storage tank (1) is 20L.

5. A small movable liquid sterile filtration device according to claim 1, characterized in that: The size of the filter element in the liquid filter (2) is 5 inches.

6. A small movable liquid sterile filtration device according to claim 1, characterized in that: The size of the filter pores of the filter element in the liquid filter (2) is 0.22 μm.

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