Automatic Raman spectrum detection equipment

Abstract

The utility model provides automatic Raman spectrum detection equipment which comprises a detection box, an air purification device is mounted on the detection box, a reagent filling device is arranged on one side of the detection box, a detection unit and a rotary table arranged corresponding to the detection unit are arranged in the detection box, and a baffle plate, an ultraviolet lamp and a fluorescent lamp are arranged on the inner wall of the detection box; the reagent filling device comprises a plurality of injectors, a liquid inlet of each injector is connected with a liquid inlet pipe, and a liquid outlet of each injector is connected with a liquid outlet pipe; the detection unit comprises a cover dismounting device, a detection device, an injection liquid adding device, a pressure maintaining device and a liquid transferring device. By arranging the sealed detection box, it can be effectively guaranteed that detection in the box is completely sterile, through cooperation of all the working devices, sample tubes containing traditional Chinese medicine test solutions are subjected to automatic cover removal, liquid relief, pressure filtration, liquid adding, natural drying and detection, interference of human factors is reduced, and the detection efficiency is greatly improved.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial detection, and in particular relates to an automated Raman spectrum detection device. Background Art

[0002] Chinese herbal medicines are prone to carry or introduce a large number of microorganisms during the growth, harvesting, processing, transportation and storage, which affects the quality stability. The metabolites of microorganisms (such as some toxins) may also harm users. In addition, the presence of pathogenic microorganisms may cause users to be infected again. Therefore, detecting the microorganisms contained in Chinese herbal medicine samples is an important factor in controlling their quality and drug safety. Due to the complex composition of Chinese herbal medicine samples, in order to obtain accurate trace microbial information in the samples, necessary pretreatment and signal tracing must be performed. Membrane filtration is a commonly used pretreatment technology. By selecting membranes of different scales, effective enrichment of samples of different scales can be achieved. The Raman spectra formed by different microorganisms after light scattering will present unique fingerprint information of microorganisms, reflecting the differences in molecular structure and physiological activity of microorganisms. Therefore, this spectral technology with rapid and sensitive "fingerprint" recognition capabilities has been applied in the existing field of pathogen detection. Most of the existing technologies rely on manual assistance to complete the detection, which is inefficient and the accuracy of the test results is not easy to guarantee. Therefore, it is necessary to design an automated detection equipment. Obtaining its microbial population information is not only of great significance for the risk assessment of microbial contamination of traditional Chinese medicine samples, but also can provide reference data for the formulation of quality control standards for microorganisms in traditional Chinese medicines. Summary of the invention

[0003] In view of this, the present invention aims to overcome the defects in the prior art and proposes an automated Raman spectroscopy detection device.

[0004] To achieve the above object, the technical solution created by the present invention is implemented as follows:

[0005] An automated Raman spectrum detection device comprises a detection box, an air purification device is installed on the detection box, and a reagent filling device, a nitrogen tank and an electric control cabinet are arranged on one side of the detection box; a detection unit and a turntable arranged corresponding to the detection unit are arranged in the detection box, and an ultraviolet lamp is arranged on the inner wall of the detection box; the reagent filling device comprises a plurality of syringes, the liquid inlet of each syringe is connected to a liquid inlet pipe, the liquid outlet is connected to a liquid outlet pipe, the liquid inlet pipeline is connected to a reagent tank, and the liquid outlet pipeline is connected to an injection liquid adding device in the detection box; the detection unit comprises a cover removal device, a detection device, an injection liquid adding device, a pressure maintaining device and a liquid transfer device, and each device is electrically connected to the electric control cabinet.

[0006] Furthermore, a plurality of supporting legs are symmetrically arranged at the bottom of the detection box.

[0007] Furthermore, the capping device includes a capping fixing plate, a capping linear module and a capping driver for driving the capping linear module to perform an action. A clamping device is installed on the capping linear module, and the clamping device is driven by the capping linear module to move in a vertical direction. The capping fixing plate is arranged below the capping linear module, and a working head for fixing the body of the sample tube is provided at its front end.

[0008] Furthermore, the detection device includes a mounting frame, a screw slide is provided on the mounting frame, and a Raman spectrometer is installed on the screw slide.

[0009] Furthermore, the injection liquid adding device comprises a fixed plate, on which a plurality of liquid adding steel needles are mounted, each of which is individually connected to a liquid adding pipeline; and a bottle cap detection device is provided on the fixed plate.

[0010] Furthermore, the pipetting device includes a pipetting linear module and a pipetting driver that drives the pipetting linear module to perform an action. A pipetting gun and a follower frame are installed on the pipetting linear module, and the pipetting gun and the follower frame are driven by the pipetting linear module to move synchronously in the vertical direction.

[0011] Furthermore, the pressure maintaining device includes a pressure maintaining cover plate installed on the cantilever of the follower frame, a pressure maintaining nozzle in the middle of the pressure maintaining cover plate, and an air blowing pipe connected to the air hole in the pressure maintaining nozzle. A compression spring is provided between the upper end surface of the pressure maintaining nozzle and the lower end surface of the cantilever. The gas in the air blowing pipe is provided by a nitrogen tank, and the opening and closing of the solenoid valve is controlled by the electric control cabinet to realize the interruption of air flow.

[0012] Further, the turntable includes a base, a turntable, a turntable driver and a turntable frame, a fixed plate is installed on the upper end of the turntable frame, the turntable is installed on the upper side of the fixed plate, the turntable driver is placed on one side of the base, a linear sliding device is provided above the fixed plate, a rotating device is provided below the fixed plate, a hollow is provided on the turntable frame, and the linear sliding device and the rotating device are driven by the turntable driver to realize the reciprocating motion and rotational motion of the turntable;

[0013] The turntable comprises a bottom plate and a hollow ring plate, the bottom plate surface is provided with a circular seat body which is concave downwards, the edge of the seat body is provided with a suction head waste liquid groove which is communicated with the concave part of the seat body, and the center is also provided with a cylindrical base body which is concave downwards, and the base body is provided with a water inlet pipe, a water outlet pipe and a waste liquid discharge pipe, an outer plate body and an inner plate body are provided above the bottom plate, a circular inner cover plate is provided on the top of the inner plate body, and an outer cover plate with a toothed edge is provided on the top of the outer plate body;

[0014] The lower surface of the outer cover plate is vortex-shaped, with a threaded water bath channel formed in the middle. The center and side of the inner cover plate are respectively provided with a water inlet and a water outlet; the edge of the outer cover plate is provided with a plurality of waste liquid ports and a plurality of filter installation slots, and every two filter installation slots correspond to a waste liquid port;

[0015] The ring disk and the inner cover plate form a waste liquid collection chamber, the outer edge of which is provided with a plurality of pipette tip slots, a plurality of sample tube slots, and pipette tip waste liquid channels corresponding to the pipette tip slots respectively, and the inner edge is provided with a plurality of filtrate channels, both of which are communicated with the waste liquid collection chamber; the sample tube slots are inserted with sample tubes, the pipette tip slots are inserted with pipette tips, and the filter mounting slots are installed with disposable filters.

[0016] Furthermore, 12 sample tube slots are evenly distributed along the circumferential direction on the outer cover plate, a pipette tip slot and a filter installation slot are provided corresponding to each sample tube slot, and a receiving slot is provided on the ring plate corresponding to the sample tube slot.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] The invention creates a sealed detection box, with air purifiers and ultraviolet lamps placed above and inside the box respectively, which reduces contact with the outside air, can effectively ensure that the detection in the box is completely sterile, and the front door switch is easy to operate, which is convenient for loading and unloading. A transparent observation plate is provided in front of the box, which can be observed in real time during the detection process, and the magnetic shading curtain provides an overall closed shading performance, which improves the precision and accuracy of equipment detection. In addition, a fluorescent lamp and a shading plate are provided in the box. The former provides lighting conditions for daily loading and unloading and garbage cleaning in the box, and the latter avoids that the detection background in the box is not affected by external light when the shading curtain is in the "open" state, thereby improving the adaptability of equipment detection. The cover removal device, detection device, injection and liquid addition device, pressure-maintaining device and liquid transfer device in the detection unit cooperate with each other to automatically remove the cover, transfer, pressurize and filter, add liquid, dry naturally and detect the sample tube containing the Chinese medicine test solution, thereby reducing the interference of human factors and greatly improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings constituting part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0020] Figure 1 A schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is a first-view schematic diagram of the detection box in the invention after the outer shell is removed;

[0022] Figure 3 This is a second viewing angle schematic diagram of the detection box in the invention after the outer shell is removed;

[0023] Figure 4 It is a structural schematic diagram of the turntable part in the invention;

[0024] Figure 5 A half-section schematic diagram of the turntable portion of the present invention;

[0025] Figure 6 It is a schematic diagram of the detection unit part in the invention.

[0026] Figure 7 Circuit control diagram created for the present invention

[0027] Figure 8 Main PLC wiring diagram. DETAILED DESCRIPTION

[0028] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0029] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0030] In the description of the invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the invention can be understood according to specific circumstances.

[0031] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0032] An automated Raman spectroscopy detection device, such as Figures 1 to 8As shown, it includes a detection box 1, on which an air purification device 10 is installed, and a reagent filling device 9, a special test computer 13, a touch screen HMI11, a nitrogen tank 8 and an electric control cabinet 12 are arranged on one side of the detection box 1; a handheld and movable touch screen HMI11 is placed above the electric control cabinet 12, and an abnormal alarm program is arranged in the HMI11; the detection box is provided with a detection unit and a turntable 2 arranged corresponding to the detection unit, and a shading plate 102, an ultraviolet lamp 101 and a fluorescent lamp 103 are provided on the inner wall of the detection box; the reagent filling device 9 includes a plurality of injectors 903, and the liquid inlet of each injector 903 is connected to a liquid inlet pipe 902, and the liquid outlet is connected to a liquid outlet pipe 503, the liquid inlet pipeline 902 is connected to the reagent tank 901, and the liquid outlet pipeline is connected to the injection and liquid adding device 9 in the detection box 1.

[0033] The detection unit includes a capping device 3, a detection device 4, an injection and liquid adding device 5, a pressure-maintaining device 6 and a liquid transfer device 7. Each device is placed on a fixed column 17 and connected to the electric control cabinet 12 through a line 14. Of course, the inner wall of the detection box is provided with ultraviolet lamps 101, fluorescent lamps 103 and air purifiers 10 and other components that require electricity or electric control, which are also connected to the electric control cabinet 12; the HMI is used to receive user instructions and realize human-computer data interaction; the electric control cabinet is provided with a PLC of the Easy522-0808 model. According to the instructions transmitted by the HMI, the PLC controls the drivers of each device to sequentially drive the air purification device 10, the reagent filling device 9, the capping device 3, the detection device 4, the injection and liquid adding device 5, the pressure-maintaining device 6 and the liquid transfer device 7 to perform corresponding actions to realize the operation of the equipment.

[0034] An operation window 104 is provided on the front door panel 106 of the detection box 1, and a magnetic light-shielding curtain 105 is installed at the operation window 104. A plurality of supporting feet 16 are symmetrically provided at the bottom of the detection box 1. The capping device 3 comprises a capping fixing plate 301, a capping linear module 302, and a capping driver 303 for driving the capping linear module 302 to perform an action. A clamping device 304 is installed on the capping linear module, and an arc-shaped capping fixing plate 301 is fixedly connected below. There is a notch at the center of the plate tip of the arc-shaped plate, and its size matches the tube body. The notch clamps the exposed sample tube body 201 on the turntable, and the capping linear module 302 drives the clamping device 304 to move in the vertical direction to realize capping.

[0035] The detection device 4 includes a mounting frame 401 and a micro slide 402 that is movably connected to the mounting frame 401 and can move laterally, and a Raman spectrometer 403 is installed on the micro slide 402. The injection liquid adding device 5 includes a fixed plate 501, on which a plurality of liquid adding steel needles 502 are installed, and each liquid adding steel needle 502 is individually connected to a liquid adding pipeline 503; and a bottle cap detection device 504 is provided on the fixed plate.

[0036] The pipetting device 7 includes a pipetting linear module 701 and a pipetting driver 702 for driving the pipetting linear module 701 to perform an action. A pipetting gun 703 and a follower frame 704 are installed on the pipetting linear module 701. The pipetting linear module 701 drives the pipetting gun 703 and the follower frame 704 to move synchronously in the vertical direction.

[0037] The pressure-maintaining device 6 includes a pressure-maintaining cover plate 601 and an air blowing pipe 602 connected to an air hole 605 in the middle of the pressure-maintaining cover plate 601. The gas in the pipe is provided by a nitrogen tank 8 and the electromagnetic valve 603 is driven to open and close through an electric control cabinet 12 to control the free on and off of the air flow. A compression spring 604 is provided between the upper end surface of the pressure-maintaining cover plate 601 and the lower end surface of the cantilever.

[0038] The turntable 2 includes a base 202, a turntable 204, a turntable driver 210 and a turntable frame 205. A fixed disk 206 is installed on the upper end of the turntable frame 205. The turntable 204 is installed on the upper side of the fixed disk 206. The turntable driver 210 is placed on one side of the base 202. A rotating device 207 is provided above the fixed disk 206 and a linear sliding device 203 is provided below the fixed disk 206. Both are arranged on the inner side of the turntable frame 205. The linear sliding device 203 and the rotating device are driven by the turntable driver 210. 207 is driven to realize the reciprocating motion and rotational motion of the turntable 204, and an elliptical hollow 208 is provided on the turntable frame 205; the turntable 204 includes a bottom plate 209 and a hollow ring plate 230, and a threaded outer disk body and a cylindrical inner disk body are provided on the bottom plate 209, a circular inner cover plate 211 is provided on the top of the inner disk body, and a toothed edge outer cover plate 212 is provided on the top of the outer disk body, and the lower surface of the outer cover plate 212 is vortex-shaped and is engaged with the inner cover plate 211 to form a water bath channel 215 in the middle.

[0039] A water inlet 216 and a water outlet 217 are respectively provided at the center and the side of the inner cover plate 211. The water inlet and outlet pipes pass through the bottom plate 209 and are respectively communicated with the hot water outlet 221 and the return water inlet 222 on the hot water tank cover plate 220. A temperature control sensor 223 and a water heater 224 are provided on the hot water tank cover plate 220. The hot water circulation pump 218 on the fixed column 17 is driven by the turntable driver 210 to pump hot water from the hot water tank 219 to the water bath channel 215, and transport the hot water cooled in the water bath channel 215 back to the hot water tank 219.

[0040] The inner cover plate 211 is fixedly connected to the bottom plate 209 and the ring disk 230 to form a waste liquid collection chamber 213, and an inlet and outlet pipe channel and a waste liquid discharge port 214 are provided at the center of the bottom plate; the outer cover plate 212 is provided with a plurality of wash pipe waste liquid discharge ports 214 and a plurality of filter mounting grooves 225, and the ring disk 230 is provided with a plurality of test tube slots 226 with half-exposed tube bodies, a plurality of pipette tip slots 227 and a plurality of filtrate channels 231, the test tube slots 226 are inserted with test tubes 201, the pipette tip slots 227 are inserted with pipette tips 228, and the filter mounting grooves 225 are installed with disposable filters 229.

[0041] The specific workflow of using this equipment for microbial testing is: preparation - adding samples - adding test reagents - natural drying and testing - finishing work. The details are as follows:

[0042] During the preparation process, the front door panel 106 is in the open state. The tester places the sterile sealed test tube 201 containing the test solution of the Chinese medicine product to be tested on the sample tube slot 226, and places multiple sterile pipette tips 228 and disposable filters 229 on the pipette tip slot 227 and the filter seat 225 respectively. Then, the sterilized detection reagents 4-mercaptophenylboric acid solution, ammonium bicarbonate eluent and Au@AgNPs sol solution are added to the reagent filling device 9 respectively. After that, the front door panel 106 is closed to ensure the sealing and light-shielding performance of the detection box 1, the ultraviolet lamp 101 and the air purifier 10 are turned on, and after sterile ventilation and ultraviolet irradiation for 30 minutes, the ultraviolet lamp 101 and the air purifier 10 are turned off and the nitrogen tank valve 14 is opened. When the gas pressure reaches 0.01Mpa-0.06Mpa, the preparation before the test is completed.

[0043] During the sample addition process, the turntable 2 moves linearly to the leftmost end, so that the notch in the center of the arc-shaped screw-cover fixing plate 301 clamps the sealed test tube 201 on the No. 1 acupoint, and the clamping claw of the clamping device 304 clamps the cap of the Chinese medicine sample liquid test tube 201 below. As the slider of the screw-cover linear module 302 slides vertically upward, the clamping claw moves upward to pull out the cap, and then the uncovered Chinese medicine sample liquid test tube 201 is sent to the bottle cap detection device 504 for screening. After passing the screening, the No. 1 acupoint displacement liquid pipette head 228 is moved to the liquid collection position (directly below the liquid transfer device), and a liquid transfer pipette head 228 is picked up and docked by the liquid transfer gun 703, and the No. 1 uncovered Chinese medicine sample liquid test tube 201 is sent to the liquid collection position (directly below the liquid transfer device). Afterwards, the pipetting driver 702 drives the pipette gun 703 to move downward to the corresponding position of the Chinese medicine sample liquid test tube 201, and uses the pipette tip 228 to absorb 1 mL of Chinese medicine sample liquid in the corresponding test tube, and transfers the sample liquid to the filter 229 (filter membrane), and then the pipetting driver 702 drives the pipette gun 703 to put the tip 228 back to the original acupuncture point, and the uncovered Chinese medicine sample liquid test tube 201 is sent to the bottom of the capping device 3. Similarly, the capping driver 303 drives the covered clamp 304 to move downward, and then close the tube cover. Finally, the pressure maintaining device 6 blows air at the filter 229 for 30 seconds to achieve rapid filtration. This process is repeated many times to add the Chinese medicine sample liquid in all sample test tubes 201 to the filter membrane in turn.

[0044] In the process of adding detection reagent, the 4-mercaptophenylboric acid solution is transported from the reagent tank 901 to the liquid adding pipeline 902 through the syringe 903, and then 0.5 mL of 4-mercaptophenylboric acid is added to the filter membrane of the filter 229 through the liquid adding steel needle 502. After standing and incubating for 15 minutes, the 4-mercaptophenylboric acid and the bacteria are fully combined. Then, the filter 229 is blown and filtered again for 30 seconds through the pressure maintaining device 6. After that, 3 mL of ammonium bicarbonate eluent and 1 mL of Au@AgNPs sol solution are added to the filter membrane of the filter 229 through the corresponding liquid adding steel needle 502. Each time the reagent is added, the pressure maintaining device 6 will blow and filter for 30 seconds.

[0045] In the natural drying and detection process, the temperature control component adjusts the temperature through the temperature control sensor 223 and the water heater 224 in the hot water tank 219, and pumps water of suitable temperature from the water inlet 216 on the turntable and discharges it from the cold water outlet 217, thereby forming a hot water circulation, and then heating the filter fixing plate 212 to achieve the insulation of the filter 229, so that the filter membrane can be quickly dried naturally for 2-5 minutes, and then the linear sliding device 203 and the rotating device 203 are driven by the turntable driver 210 to realize the migration of the turntable 204, and the turntable 2 moves the dried filter membrane to the bottom of the laser 403, and finally the laser 403 emits the laser, and the detection device 4 collects the spectrum of each filter membrane in turn.

[0046] After the test is completed, the tester performs finishing work, opens the front door panel 106, transfers the disposable filter 229, the Chinese medicine sample tube 201 and the waste pipette tip 228 on the turntable 2 from the inside of the test box 1 to the outside for removal, opens the waste liquid discharge port 214, discharges the waste liquid in the waste liquid collection chamber 213, and turns off the hot water circulation pump 218, then places the new disposable filter 229, the pipette tip 228 and the untested Chinese medicine sample tube 201 in the corresponding installation points, and then a new round of testing can be carried out.

[0047] For example, the outer cover plate 212 is provided with 12 test tube slots 226, 12 pipette tip slots 227 and 12 filter installation slots 225, that is, 12 pipette tips 228, 12 sterile sealed sample test tubes 201 and 12 filters 229 are installed on the turntable 2, and 12 different samples can be tested at the same time. The operation is simple, the detection area is strictly shielded from light, the detection accuracy and automation are high, and the large-scale detection efficiency is high and the cost is low.

[0048] The invention creates a sealed detection box with an air purifier and an ultraviolet lamp placed above and inside the box respectively, thereby reducing contact with the outside air and effectively ensuring that the detection inside the box is completely sterile. The front door is easy to operate and convenient for loading and unloading. A transparent observation panel is provided in front of the box, which can be used for real-time observation during the detection process. The magnetic blackout curtain provides an overall closed blackout performance, thereby improving the precision and accuracy of equipment detection.

[0049] In addition, the box is equipped with fluorescent lamps and shading boards. The former provides lighting conditions for daily loading and unloading of samples and cleaning of garbage in the box, and the latter prevents the detection background in the box from being affected by external light when the shading curtain is in the "open" state, thereby improving the adaptability of equipment detection. The decapping device, detection device, injection and liquid adding device, pressure-maintaining device and liquid transfer device in the detection unit cooperate with each other to automatically decap, transfer, pressurize and filter, add liquid, dry naturally and detect the sample tubes containing the Chinese medicine test solution, reducing interference from human factors and greatly improving detection efficiency.

[0050] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An automated Raman spectroscopy detection device, characterized in that: It comprises a detection box, on which an air purification device is installed, and a reagent filling device, a nitrogen tank and an electric control cabinet are arranged on one side of the detection box; a detection unit and a turntable arranged corresponding to the detection unit are arranged in the detection box, and a shading plate and an ultraviolet lamp are arranged on the inner wall of the detection box; the reagent filling device comprises a plurality of syringes, the liquid inlet of each syringe is connected with a liquid inlet pipe, the liquid outlet is connected with a liquid outlet pipe, the liquid inlet pipeline is connected with the reagent tank, and the liquid outlet pipeline is connected with the injection and liquid adding device in the detection box; the detection unit comprises a cover removal device, a detection device, an injection and liquid adding device, a pressure maintaining device and a liquid transfer device, and each device is electrically connected with the electric control cabinet.

2. The automated Raman spectroscopy detection device according to claim 1, characterized in that: A plurality of supporting legs are symmetrically arranged at the bottom of the detection box.

3. The automated Raman spectroscopy detection device according to claim 1, characterized in that: The capping device includes a capping fixing plate, a capping linear module and a capping driver for driving the capping linear module to perform an action. A clamping device is installed on the capping linear module, and the clamping device is driven by the capping linear module to move in the vertical direction. The capping fixing plate is arranged below the capping linear module, and a working head for fixing the body of the sample tube is provided at its front end.

4. The automated Raman spectroscopy detection device according to claim 1, characterized in that: The detection device comprises a mounting frame, a screw slide is arranged on the mounting frame, and a Raman spectrometer is installed on the screw slide.

5. The automated Raman spectroscopy detection device according to claim 1, characterized in that: The injection liquid adding device comprises a fixed plate, on which a plurality of liquid adding steel needles are installed, each of which is individually connected to a liquid adding pipeline; and a bottle cap detection device is arranged on the fixed plate.

6. The automated Raman spectroscopy detection device according to claim 1, characterized in that: The pipetting device comprises a pipetting linear module and a pipetting driver for driving the pipetting linear module to perform an action. A pipetting gun and a follower frame are installed on the pipetting linear module, and the pipetting gun and the follower frame are driven by the pipetting linear module to move synchronously in the vertical direction.

7. The automated Raman spectroscopy detection device according to claim 1, characterized in that: The pressure maintaining device includes a pressure maintaining cover plate installed on the cantilever of the follower frame, a pressure maintaining nozzle in the middle of the pressure maintaining cover plate, and an air blowing pipe connected to the air hole in the pressure maintaining nozzle. A compression spring is provided between the upper end surface of the pressure maintaining nozzle and the lower end surface of the cantilever. The gas in the air blowing pipe is provided by a nitrogen tank, and the opening and closing of the solenoid valve is controlled by the electric control cabinet to realize the interruption of gas flow.

8. The automated Raman spectroscopy detection device according to claim 1, characterized in that: The turntable comprises a base, a turntable, a turntable driver and a turntable frame, a fixed plate is mounted on the upper end of the turntable frame, the turntable is mounted on the upper side of the fixed plate, the turntable driver is placed on one side of the base, a linear sliding device is arranged above the fixed plate, a rotating device is arranged below the fixed plate, a hollow is arranged on the turntable frame, and the linear sliding device and the rotating device are driven by the turntable driver to realize the reciprocating motion and the rotating motion of the turntable; The turntable comprises a bottom plate and a hollow ring plate, the bottom plate surface is provided with a circular seat body which is concave downwards, the edge of the seat body is provided with a suction head waste liquid groove which is communicated with the concave part of the seat body, and the center is also provided with a cylindrical base body which is concave downwards, and the base body is provided with a water inlet pipe, a water outlet pipe and a waste liquid discharge pipe, an outer plate body and an inner plate body are provided above the bottom plate, a circular inner cover plate is provided on the top of the inner plate body, and an outer cover plate with a toothed edge is provided on the top of the outer plate body; The lower surface of the outer cover plate is vortex-shaped, with a threaded water bath channel formed in the middle. The center and side of the inner cover plate are respectively provided with a water inlet and a water outlet; the edge of the outer cover plate is provided with a plurality of waste liquid ports and a plurality of filter installation slots, and every two filter installation slots correspond to a waste liquid port; The ring disk and the inner cover plate form a waste liquid collection chamber, the outer edge of which is provided with a plurality of pipette tip slots, a plurality of sample tube slots, and pipette tip waste liquid channels corresponding to the pipette tip slots respectively, and the inner edge is provided with a plurality of filtrate channels, both of which are communicated with the waste liquid collection chamber; the sample tube slots are inserted with sample tubes, the pipette tip slots are inserted with pipette tips, and the filter mounting slots are installed with disposable filters.

9. The automated Raman spectroscopy detection device according to claim 8, characterized in that: The outer cover plate is evenly distributed with 12 sample tube slots along the circumferential direction, each sample tube slot is provided with a pipette tip slot and a filter installation slot, and the ring plate is provided with a receiving slot corresponding to the sample tube slot.

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