POCT blood cell analyzer

Abstract

The application discloses a POCT hematology analyzer, a casing of the POCT hematology analyzer is provided with a detection position, the detection position is used for placing a detection box, and the detection box is loaded with a sample; the reagent storage assembly is used for storing a detection reagent; the pipetting assembly is used for adding a detection reagent into the detection box, so that a sample reacts with the detection reagent and a to-be-detected sample solution is obtained; the detection assembly is used for carrying out counting test on the to-be-detected sample liquid in the detection box to obtain a test result. The reagent storage assembly is arranged in the casing of the POCT hematology analyzer, so that the pipetting assembly can directly perform sample preparation through the detection reagent in the machine, the structural space of the detection box is saved, the process of the detection box is simple, the cost of the detection box is reduced, and the practicability of the detection box is improved.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a POCT blood cell analyzer. Background Art

[0002] At present, when a POCT blood cell analyzer performs blood cell testing through a test box, the test box usually stores the reagents and consumables required for the test. The structure and process of the test box are relatively complex, and the reagents of the test box need to be packaged through processes such as filling and sealing. The test box also needs to reserve space for pre-packaged diluents and hemolytic agents. The structural space of the test box is large, the manufacturing time is long, and the manufacturing cost of the test box is high. Utility Model Content

[0003] In order to solve the technical problem of high manufacturing cost of the test kit in the prior art, the present application provides a POCT blood cell analyzer.

[0004] In order to solve the technical problems existing in the prior art, the present application provides a POCT blood cell analyzer, which includes a housing, a reagent storage component, a pipetting component and a detection component. The housing is provided with a detection position, and the detection position is used to place a detection box, and the detection box is loaded with a sample; the reagent storage component is arranged in the housing, and is used to store the detection reagent; the pipetting component is arranged in the housing, and is used to add the detection reagent to the detection box, so that the sample reacts with the detection reagent and obtains a sample liquid to be tested; the detection component is arranged in the housing, and is used to perform a counting test on the sample liquid to be tested in the detection box to obtain a test result.

[0005] Optionally, the reagent storage assembly includes a first reagent storage bottle and a second reagent storage bottle, the first reagent storage bottle is used to store a diluent, the second reagent storage bottle is used to store a hemolytic agent, and the pipetting assembly is used to add at least one of the diluent and the hemolytic agent to the detection box.

[0006] Optionally, the detection box includes a sample pool, a detection pool and a dilution pool, the sample pool is used to hold the sample, and the detection pool and the dilution pool are arranged on one side or two opposite sides of the sample pool; the pipetting component is used to add the diluent to the dilution pool and the detection pool respectively, and the pipetting component is also used to transfer the sample from the sample pool to the dilution pool and transfer the liquid from the dilution pool to the detection pool, so that the detection pool stores a first sample liquid for detecting a first item, and the pipetting component is also used to add the hemolytic agent to the dilution pool, so that the dilution pool stores a second sample liquid for detecting a second item.

[0007] Optionally, the detection box includes a sample pool and a detection pool, the sample pool is used to hold the sample, the detection pool is arranged on one side of the sample pool, and the pipetting component is used to add the diluent and the sample to the detection pool respectively, or the pipetting component is used to add the diluent, the sample and the hemolytic agent to the detection pool respectively to obtain the sample liquid to be tested.

[0008] Optionally, the number of the detection cells is at least one.

[0009] Optionally, the reagent storage assembly also includes a movable clamp, which is arranged on the housing, and the first reagent storage bottle and the second reagent storage bottle include a bottle body and a bottle cap, the bottle cap is used to seal the detection reagent stored in the bottle body, and the movable clamp is used to open or close the bottle caps of the first reagent storage bottle and the second reagent storage bottle.

[0010] Optionally, the POCT blood cell analyzer also includes a pipette tip storage assembly, which is disposed in the housing and is used to store a plurality of pipette tips. The pipette tip storage assembly is used to move to the pipette tip storage assembly and plug in the pipette tip to perform liquid transfer operations through the pipette tip.

[0011] Optionally, the pipette tip storage assembly includes a first storage position and a second storage position, the first storage position is provided with at least one first pipette tip, the second storage position is provided with at least one second pipette tip, the pipetting assembly is used to insert the first pipette tip from the first storage position and to absorb the diluent of the reagent storage assembly through the first pipette tip, and the pipetting assembly is also used to insert the second pipette tip from the second storage position and to absorb the hemolytic agent of the reagent storage assembly through the second pipette tip.

[0012] Optionally, the POCT blood cell analyzer further comprises a remaining amount sensor, which is disposed in the reagent storage component and is used to detect the reagent remaining amount in the reagent storage component to generate a warning message when the reagent remaining amount is lower than a preset threshold value.

[0013] Optionally, the detection box comprises a sample pool, a detection pool and a dilution pool, and the sample pool, the detection pool and the dilution pool are arranged in a linear direction.

[0014] Compared with the prior art, the housing of the POCT blood cell analyzer of the present application is provided with a detection position, the detection position is used to place the detection box, and the detection box is loaded with a sample; the reagent storage component is used to store the detection reagent; the liquid transfer component is used to add the detection reagent to the detection box, so that the sample reacts with the detection reagent and obtains the sample liquid to be tested; the detection component is used to perform a counting test on the sample liquid to be tested in the detection box to obtain the test result. The present application integrates the reagent storage component into the housing of the POCT blood cell analyzer, so that the liquid transfer component can directly prepare the sample through the detection reagent in the machine, saving the structural space of the detection box, and the process of the detection box is simple, which is conducive to reducing the cost of the detection box and improving the practicality of the detection box. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a structural schematic diagram of the first embodiment of the POCT blood cell analyzer provided by the present application;

[0017] Figure 2 yes Figure 1 A structural schematic diagram of an embodiment of a detection box;

[0018] Figure 3 It is a structural schematic diagram of the second embodiment of the POCT blood cell analyzer provided by the present application. DETAILED DESCRIPTION

[0019] The present application is further described in detail below in conjunction with the accompanying drawings and examples. It is particularly noted that the following examples are only used to illustrate the present application, but are not intended to limit the scope of the present application. Similarly, the following examples are only some embodiments of the present application rather than all embodiments, and all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present application.

[0020] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0021] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "set", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or connected through an intermediate medium. For ordinary technicians in this field, if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of this application, the directional indications are only used to explain the relative position relationship, movement, etc. between the components in a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0022] See also Figure 1 , Figure 1 Schematic diagram of the structure of the first embodiment of the POCT blood cell analyzer provided by the present application. Figure 1 As shown, an embodiment of the present application provides a POCT blood cell analyzer, which includes a housing 11, a reagent storage component 12, a pipetting component 13 and a detection component (not shown).

[0023] The housing 11 of the POCT blood cell analyzer is provided with a detection position, which is used to place a detection box 110, and the detection box 110 is loaded with a sample; a reagent storage component 12 is provided in the housing 11, and is used to store detection reagents; a pipetting component 13 is provided in the housing 11, and is used to add detection reagents to the detection box 110, so that the sample reacts with the detection reagent and obtains a sample liquid to be tested; a detection component is provided in the housing 11, and is used to perform a counting test on the sample liquid to be tested in the detection box 110 to obtain a test result.

[0024] Specifically, the housing 11 is used to support the reagent storage component 12, the pipetting component 13 and the detection component, so that the other components of the POCT blood cell analyzer can be accurately placed in the corresponding position and realize the corresponding function, and the housing 11 is also used to shield the reagent storage component 12, the pipetting component 13 and the detection component, so that each work flow of the POCT blood cell analyzer can be executed in a closed environment, reducing external interference and sample leakage risks. The housing 11 is provided with a detection position, and the detection position is used to place the detection box 110, and the detection box 110 includes at least one accommodating cavity for loading the sample. Among them, the detection position can be set at a preset receiving position of the housing 11, for example, the housing 11 can include a housing 11 body and a detection seat connected to the housing 11 body in a sliding or detachable manner, and the detection seat is used to place the detection box 110. The sample is a blood sample obtained after the subject is subjected to a blood sample collection operation, and can also be a diluted sample after the blood sample is pre-diluted. The specific position of the detection position and the sample type are not specifically limited here.

[0025] The reagent storage component 12 and the pipetting component 13 are arranged on the housing 11, and the reagent storage component 12 is used to store the detection reagent. The pipetting component 13 may include a pipetting head and a driving member, and the pipetting component 13 is used to absorb the sample and the detection reagent through the pipetting head, and the driving member is used to drive the pipetting head to move. The pipetting component 13 can absorb the detection reagent on the reagent storage component 12 through the pipetting head, and the driving member is used to drive the pipetting head to lift the needle and move the pipetting head to the detection box 110, so that the pipetting head discharges the absorbed detection reagent to a certain position of the detection box 110, so that the sample on the detection box 110 reacts with the detection reagent and obtains the sample liquid to be tested. Among them, the detection reagent can be a hemolytic agent, a dye solution and other reagents used in different detection methodologies, and the type of the detection reagent may be related to the detection items adapted by the POCT blood cell analyzer, etc., and is not specifically limited here.

[0026] In a possible embodiment, the pipetting component 13 is used to transfer the detection reagent, and can also be used to transfer the sample liquid to be tested after the reaction is completed to other cells of the detection box 110. In other embodiments, the detection box 110 may include multiple cells, and the pipetting component 13 can also absorb the sample in a cell body through the pipetting head, and transfer the sample in the cell body to another cell body, so that the pipetting head adds the detection reagent to the two cells respectively, so as to obtain the sample liquid to be tested for different detection items respectively.

[0027] In the embodiment of the present application, the housing 11 of the POCT blood cell analyzer is provided with a detection position, the detection position is used to place the detection box 110, and the detection box 110 is loaded with a sample; the reagent storage component 12 is provided in the housing 11, and is used to store the detection reagent; the pipetting component 13 is provided in the housing 11, and is used to add the detection reagent to the detection box 110, so that the sample reacts with the detection reagent and obtains the sample liquid to be tested; the detection component is provided in the housing 11, and is used to perform a counting test on the sample liquid to be tested in the detection box 110 to obtain the test result. The POCT blood cell analyzer of this embodiment has the reagent storage component 12 built into the housing 11, so that the pipetting component 13 can directly prepare the sample through the detection reagent in the machine, and there is no need to set a pool body for storing the detection reagent on the detection box 110, which saves the structural space of the detection box 110, and the process of the detection box 110 is simple, which is conducive to reducing the cost of the detection box 110 and improving the practicality of the detection box 110.

[0028] In one embodiment, the reagent storage component 12 includes a first reagent storage bottle (not shown) and a second reagent storage bottle (not shown), the first reagent storage bottle is used to store a diluent, the second reagent storage bottle is used to store a hemolytic agent, and the pipetting component 13 is used to add at least one of the diluent and the hemolytic agent to the detection box 110.

[0029] Specifically, the reagent storage assembly 12 includes a first reagent storage bottle and a second reagent storage bottle. The first reagent storage bottle is used to store a diluent, which is an isotonic solution with an acid-base buffering effect, appropriate ionic strength and conductivity. The diluent can usually be used to dilute the sample to adjust the gap between the blood cells in the sample. The second reagent storage bottle is used to store a hemolytic agent, which is used to dissolve the red blood cells in the sample to leak the contents of the red blood cells. It can be understood that when the detection methodology or detection items applicable to the POCT blood cell analyzer are different, the detection reagents stored in the reagent storage assembly 12 can be different.

[0030] Illustratively, in one embodiment, the detection component is used to count red blood cells or platelets of a sample by an electrical impedance method, the reagent storage component 12 may include a first reagent storage bottle for storing a diluent, and the pipetting component 13 is used to add the diluent of the first reagent storage bottle to the detection box 110; in another embodiment, the detection component is used to count white blood cells of a sample, the detection reagent may include a diluent and a hemolyzing agent, the pipetting component 13 is used to add the diluent and the hemolyzing agent to the detection box 110, and the hemolyzing agent is used to dissolve the red blood cells in the sample to reduce the interference of the red blood cells on the white blood cell count; in other embodiments, the detection component is used to count hemoglobin of a sample by an optical method or an electrical impedance method, in which case the detection reagent may include a diluent and a hemolyzing agent, the pipetting component 13 is used to add the diluent and the hemolyzing agent to the detection box 110, and the hemolyzing agent is used to dissolve the red blood cells in the sample to release the hemoglobin in the red blood cells and count the hemoglobin.

[0031] In the embodiment of the present application, the first reagent storage bottle of the reagent storage component 12 is used to store the diluent, the second reagent storage bottle is used to store the hemolytic agent, and the pipetting component 13 is used to add at least one of the diluent and the hemolytic agent to the detection box 110, so that the pipetting component 13 can directly prepare the sample through the detection reagent in the machine. There is no need to set a pool body for storing the detection reagent on the detection box 110, which saves the structural space of the detection box 110. The process of the detection box 110 is simple, which is conducive to reducing the cost of the detection box 110 and improving the practicality of the detection box 110.

[0032] In an alternative embodiment, if Figure 1As shown, the detection box 110 includes a sample pool 111, a detection pool 112 and a dilution pool 113. The sample pool 111 is used to carry samples. The detection pool 112 and the dilution pool 113 can be arranged on the same side of the sample pool 111, or the detection pool 112 and the dilution pool 113 can be respectively arranged on opposite sides of the sample pool 111, or the sample pool 111, the detection pool 112 and the dilution pool 113 can be arranged in a triangular position. The specific arrangement order or arrangement position of the sample pool 111, the detection pool 112 and the dilution pool 113 can be set according to the travel path of the pipetting component 13, the pipetting efficiency, etc., and is not specifically limited here.

[0033] The pipetting component 13 is used to add diluent to the dilution pool 113 and the detection pool 112 respectively. The pipetting component 13 is also used to transfer the sample in the sample pool 111 to the dilution pool 113 and to transfer the liquid in the dilution pool 113 to the detection pool 112, so that the detection pool 112 stores a first sample liquid for detecting a first item. The pipetting component 13 is also used to add a hemolytic agent to the dilution pool 113, so that the dilution pool 113 stores a second sample liquid for detecting a second item.

[0034] Specifically, when the detection box 110 includes a sample pool 111, a detection pool 112 and a dilution pool 113, the pipetting component 13 is used to add the diluent in the first reagent storage bottle to the dilution pool 113 and the detection pool 112 respectively. The pipetting component 13 is also used to transfer the sample carried by the sample pool 111 to the dilution pool 113, so that the diluent in the dilution pool 113 dilutes the sample once. The pipetting component 13 continues to be used to transfer the sample diluted once in the dilution pool 113 to the detection pool 112, so that the dilution in the detection pool 112 dilutes the second sample liquid for a second time and obtains the first sample liquid for detecting the first item. The pipetting component 13 is used to add the hemolytic agent in the second reagent storage bottle to the dilution pool 113, so that the hemolytic agent can react with the sample in the dilution pool 113 and obtain the second sample liquid for detecting the second item.

[0035] Among them, the first sample liquid is the sample liquid to be tested after secondary dilution, and the first sample liquid can be used for counting cells of a larger order of magnitude, for example, the first sample liquid can be used for counting red blood cells and / or platelets; the second sample liquid is the sample liquid to be tested formed after red blood cell lysis treatment, and the second sample liquid can be used for counting cells of non-red blood cells or red blood cell contents, for example, the second sample liquid can be used for counting white blood cells and / or hemoglobin. That is, the first item includes but is not limited to red blood cells and / or platelets, and the second item includes but is not limited to white blood cells and / or hemoglobin. At this time, the detection component of the POCT blood cell analyzer may include an impedance detection component and an optical detection component, the impedance detection component is used to connect with the detection pool 112 of the detection box 110 and perform impedance counting on the first sample liquid and / or the second sample liquid of the detection pool 112, and the optical detection component is used to irradiate the second sample liquid on the detection pool 112 with a laser to obtain optical information of the second sample liquid.

[0036] In an embodiment of the present application, the detection box 110 may include a sample pool 111, a detection pool 112 and a dilution pool 113, so that the POCT blood cell analyzer of this embodiment can perform sample detection of the first item and the second item through the detection box 110, thereby improving the applicability of the POCT blood cell analyzer in different detection scenarios, and the detection box 110 only needs to be provided with a few pool bodies for storing the sample liquid to be tested and the original sample without storing the detection reagent, thereby saving the structural space of the detection box 110 and reducing the process difficulty of the detection box 110, which is conducive to reducing the cost of the detection box 110.

[0037] In another alternative embodiment, see Figure 2 , Figure 2 yes Figure 1 A schematic diagram of the structure of an embodiment of the detection box. Figure 2 As shown, the detection box 110 includes a sample pool 111 and a detection pool 112, the sample pool 111 is used to carry the sample, the detection pool 112 is arranged on one side of the sample pool 111, and the pipetting component 13 is used to add the diluent and the sample to the detection pool 112 respectively, or the pipetting component 13 is used to add the diluent, the sample and the hemolytic agent to the detection pool 112 respectively to obtain the sample liquid to be tested.

[0038] Specifically, when the detection box 110 includes the sample pool 111 and the detection pool 112, the detection component can be used to test a certain detection item on the sample. Exemplarily, when the detection component is used to perform a first item of testing on the sample, the pipetting component 13 is used to add the diluent of the first reagent storage bottle to the sample pool 111, so that the diluent dilutes the sample in the sample pool 111 and obtains a first sample liquid, and the pipetting component 13 is also used to transfer the quantitative first sample liquid in the sample pool 111 to the detection pool 112 for testing; or, the pipetting component 13 is used to add the diluent of the first reagent storage bottle to the detection pool 112, and the pipetting component 13 is also used to transfer the quantitative sample in the sample pool 111 to the detection pool 112, so that the diluent dilutes the sample by a preset multiple and obtains the first sample liquid; or, when the detection component is used to perform a second item of testing on the sample, the pipetting component 13 is used to add the diluent of the first reagent storage bottle, the sample in the sample pool 111 and the hemolytic agent in the second reagent storage bottle to the detection pool 112 respectively to obtain a second sample liquid.

[0039] In an embodiment of the present application, the detection box 110 may include a sample pool 111 and a detection pool 112, so that the POCT blood cell analyzer of this embodiment can perform sample detection of the first item or the second item through the detection box 110. Since the detection box 110 only needs to be provided with a sample pool 111 for storing samples and a detection pool 112 as a detection site, it can maximize the saving of the structural space of the detection box 110, reduce the use of consumables and the difficulty of process manufacturing of the detection box 110, and further reduce the cost of the detection box 110.

[0040] Furthermore, the number of the detection pool 112 is at least one.

[0041] Specifically, the detection pool 112 of the detection box 110 of this embodiment is used to store the sample liquid to be tested and serve as a detection site, so that the detection component of the POCT blood cell analyzer can test the sample liquid to be tested in the detection pool 112. The number of detection pools 112 can be one or more. Exemplarily, when the POCT blood cell analyzer is used to perform sample testing of the first item and / or the second item through the test box 110 and the number of test pools 112 is one, the test pool 112 can be used as a test site for the first item or the second item for testing, or, the POCT blood cell analyzer can clean the test pool 112 with a diluent or a cleaning solution so that the test pool 112 can be used as a test site for the first item and the second item for repeated testing, so as to save the structural space of the test box 110 and improve the space utilization of the test box 110; or, when the number of test pools 112 is multiple, the number of test pools 112 can correspond to the test items applicable to the POCT blood cell analyzer, for example, the first test pool 112 is used to test the first item, and the second test pool 112 is used to test the second item, so as to reduce the cleaning steps of the test pool 112 and improve the detection efficiency of the POCT blood cell analyzer.

[0042] Optionally, the reagent storage assembly 12 also includes a movable clamp (not shown), which is arranged on the housing 11. The first reagent storage bottle and the second reagent storage bottle include a bottle body and a bottle cap, and the bottle cap is used to seal the detection reagent stored in the bottle body. The movable clamp is used to open or close the bottle caps of the first reagent storage bottle and the second reagent storage bottle.

[0043] Specifically, the first reagent storage bottle and the second reagent storage bottle include a bottle body and a bottle cap, the bottle body is formed with a receiving cavity for storing the detection reagent, and the bottle cap is detachably connected to the bottle body and is used to seal the receiving cavity of the bottle body, so that the detection reagent is stored in a sealed space, reducing the adverse effects of dust and impurities in the external air on the detection reagent. Among them, the bottle cap can be rotatably connected to the bottle body through threaded engagement, or the bottle cap and the bottle body can be connected by a snap; when the pipetting component 13 needs to absorb the detection reagent, the mobile clamp is used to open the bottle cap of the first reagent storage bottle and / or the second reagent storage bottle, so that the pipetting head of the pipetting component 13 can extend into the receiving cavity of the bottle body and absorb the detection reagent; the mobile clamp is also used to close the bottle cap of the first reagent storage bottle and / or the second reagent storage bottle after the pipetting component 13 absorbs the detection reagent, so that the first reagent storage bottle and / or the second reagent storage bottle continue to remain sealed.

[0044] In the embodiment of the present application, the first reagent storage bottle and / or the second reagent storage bottle of the reagent storage component 12 are sealed with reagents by bottle caps, so that the detection reagent can be stored in a sealed space and the stability and reliability of the detection reagent are improved; the POCT blood cell analyzer opens or closes the bottle caps of the first reagent storage bottle and the second reagent storage bottle by moving the clamp, so that the pipetting component 13 can absorb the detection reagent in the reagent storage component 12, thereby improving the detection accuracy of the POCT blood cell analyzer.

[0045] In one embodiment, see Figure 3 , Figure 3 Schematic diagram of the structure of the second embodiment of the POCT blood cell analyzer provided by the present application. Figure 3 As shown, the POCT blood cell analyzer also includes a pipette tip 131 storage assembly, which is disposed on the housing 11 and is used to store a plurality of pipette tips 131 . The pipette tip 131 storage assembly is used to move to the pipette tip 131 storage assembly and plug in the pipette tip 131 so as to perform liquid transfer operations through the pipette tip 131 .

[0046] Specifically, the pipette head 131 storage assembly can be arranged on a side of the housing 11 close to the pipette head 13, and a plurality of pipette heads 131 are stored on the pipette head 131 storage assembly. When the POCT blood cell analyzer receives the test box 110 and starts the POCT test, the pipette head 13 is used to move to the pipette head 131 storage assembly and align with one of the pipette heads 131. The driving member of the pipette head assembly 13 is used to drive the pipette head to move downward and make the pipette head plug into the pipette head 131 under the downward force, so that the pipette head assembly 13 can perform liquid transfer operations through the pipette head 131, and the pipette head does not contact the liquid in the test box 110 and the reagent storage assembly 12, so that the pipette head assembly 13 of this embodiment does not need to be cleaned. The POCT blood cell analyzer does not need to set a corresponding cleaning pipeline on the pipette head assembly 13, and the structure is streamlined, thereby improving the space utilization of the POCT blood cell analyzer.

[0047] Optionally, the pipette tip 131 storage component includes a first storage position 141 and a second storage position 142, the first storage position 141 is provided with at least one first pipette tip 131, the second storage position 142 is provided with at least one second pipette tip 131, the pipetting component 13 is used to insert the first pipette tip 131 from the first storage position 141 and to absorb the diluent of the reagent storage component 12 through the first pipette tip 131, and the pipetting component 13 is also used to insert the second pipette tip 131 from the second storage position 142 and to absorb the hemolytic agent of the reagent storage component 12 through the second pipette tip 131.

[0048] Specifically, the pipette tip 131 storage assembly may be provided with a first storage position 141 and a second storage position 142, at least one first pipette tip 131 is placed on the first storage position 141, and at least one second pipette tip 131 is placed on the second storage position 142. In a possible embodiment, a plurality of first pipette tips 131 and a plurality of second pipette tips 131 may be nested and stacked on corresponding storage positions to reduce the space occupied by the pipette tips 131.

[0049] When the detection reagents of the POCT blood cell analyzer include diluents and hemolytic agents, the pipetting assembly 13 is used to insert the first pipette head 131 from the first storage position 141 and absorb the diluent of the first reagent storage bottle through the first pipette head 131, and the pipetting assembly 13 is also used to move to the detection box 110 and add the diluent absorbed by the first pipette head 131 to the detection box 110. The pipette head 131 storage assembly may also include an unloading position 143, and the pipetting assembly 13 is also used to separate the first pipette head 131 from the pipette head after the diluent is transferred and unload the first pipette head 131 on the unloading position 143. When the pipetting assembly 13 transfers the hemolytic agent, the pipetting assembly 13 is used to insert the second pipette head 131 from the second storage position 142 and absorb the hemolytic agent of the second reagent storage bottle through the second pipette head 131. Through the above-mentioned method, when transferring the diluent and the hemolytic agent, the pipetting component 13 of this embodiment can reduce the contamination of the detection reagent stored in the first reagent storage bottle and the second reagent storage bottle caused by the reuse of the pipette head 131, thereby improving the reliability of the detection reagent and further improving the detection accuracy of the POCT blood cell analyzer.

[0050] Further, the first storage position 141 and the second storage position 142 may be in the same position to reduce the space occupied by the pipette tip 131 storage assembly; or Figure 3 As shown, the first storage position 141 and the second storage position 142 are respectively arranged at two positions of the housing 11, so that the pipette tip 131 storage assembly can store more pipette tips 131, reduce the number of replacement times, and improve the user experience.

[0051] Furthermore, in other possible embodiments, the pipette tip 131 storage component can also be arranged on the detection box 110. For example, the detection box 110 is provided with a first storage position 141 and a second storage position 142, a first pipette tip 131 is inserted in the first storage position 141, and a second pipette tip 131 is inserted in the second storage position 142. Alternatively, two pipette tips 131 are inserted in the storage position of the detection box 110, so that the pipetting component 13 transfers the hemolytic agent and the diluent respectively through the two pipette tips 131, which is not specifically limited here.

[0052] In one embodiment, the POCT blood cell analyzer also includes a remaining amount sensor (not shown), which is arranged in the reagent storage component 12. The remaining amount sensor is used to detect the reagent remaining amount in the reagent storage component 12 to generate a warning message when the reagent remaining amount is lower than a preset threshold.

[0053] Specifically, the POCT blood cell analyzer can detect the reagent remaining amount of the reagent storage component 12 through the remaining amount sensor. For example, the POCT blood cell analyzer may include a main control component, a first remaining amount sensor is provided in the first reagent storage bottle, a second remaining amount sensor is provided in the second reagent storage bottle, the main control component is connected to the remaining amount sensor, and the main control component is used to generate a warning message when the first remaining amount sensor and / or the second remaining amount sensor indicates insufficient remaining amount.

[0054] In a possible implementation, after the main control component generates a warning message, it sends the warning message to the instrument interface of the POCT blood cell analyzer to display it to the user, or the main control component controls the corresponding warning light to work when generating the warning message, so that the user can replace the reagent storage bottle based on the warning message or the warning light; or, the reagent storage component 12 inside the POCT blood cell analyzer can be connected to the external reagent tank through a connecting pipeline, and the main control component is used to respond to the receipt of the warning message, control the corresponding valve device and allow the detection reagent in the reagent tank to enter the reagent storage component 12 through the connecting pipeline, so as to realize automatic replenishment of the detection reagent. Furthermore, the remaining amount sensor can be a contact liquid level sensor, including but not limited to a pressure differential sensor, a float sensor, a magnetic liquid level transmitter, a buoy sensor, etc.; the remaining amount sensor can also be an ultrasonic liquid level transmitter, a radar liquid level transmitter, and other non-contact sensors, which are not specifically limited here.

[0055] In an embodiment of the present application, the POCT blood cell analyzer can detect the reagent remaining amount of the reagent storage component 12 through a remaining amount sensor to ensure that the reagent storage component 12 of the POCT blood cell analyzer can maintain an appropriate reagent remaining amount and enable the pipetting component 13 to transfer the detection reagent to the detection box 110 for sample preparation, thereby improving the practicality and reliability of the POCT blood cell analyzer.

[0056] In one embodiment, if Figure 1 As shown, the detection box 110 includes a sample pool 111, a detection pool 112 and a dilution pool 113, and the sample pool 111, the detection pool 112 and the dilution pool 113 are arranged in a linear direction.

[0057] Specifically, when the detection box 110 includes a sample pool 111, a detection pool 112 and a dilution pool 113, the multiple pool bodies of the detection box 110 are arranged in a linear direction, and the first reagent storage bottle and the second reagent storage bottle of the reagent storage assembly 12 can also be arranged in the above-mentioned linear direction, so that the pipetting assembly 13 can move in a linear horizontal direction and transfer liquid between multiple pool bodies and reagent storage bottles, so that the driving member can drive the pipetting head in a certain horizontal direction and vertical direction, thereby reducing the space occupied by the pipetting drive, further reducing the structural space of the POCT blood cell analyzer, and facilitating the miniaturized application of the POCT blood cell analyzer.

[0058] Further, when the detection box 110 includes a sample pool 111, a detection pool 112 and a dilution pool 113, the POCT blood cell analyzer is used to receive the detection box 110 and open the bottle cap of the first reagent storage bottle by moving the clamp, the pipetting component 13 is used to plug the first pipette head 131, and the pipetting component 13 is also used to move to the first reagent storage bottle and suck the diluent of the first reagent storage bottle by the first pipette head 131, so as to transfer the quantitative diluent to the detection pool 112 and the dilution pool 113 of the detection box 110 respectively. The mobile clamp is used to close the bottle cap of the first reagent storage bottle. The optical detection component of the detection component is used to perform optical measurement on the diluted liquid in the detection pool 112 to obtain the optical background value of the detection pool 112; the pipetting component 13 is used to add the sample in the sample pool 111 to the dilution pool 113, and transfer the sample diluted once in the dilution pool 113 to the detection pool 112, so that the detection pool 112 stores the first sample liquid; the impedance detection component of the detection component is used to perform impedance counting on the first sample liquid in the detection pool 112 to obtain a first detection result of red blood cells and / or platelets.

[0059] The pipetting assembly 13 is also used to transfer the detection waste liquid in the detection pool 112 to the sample pool 111 or other pools of the detection box 110. The pipetting assembly 13 is used to clean the detection pool 112 or directly transfer the sample diluted once in the dilution pool 113 to the detection pool 112. The pipetting assembly 13 is used to unload the first pipette head 131 and plug the second pipette head 131. The moving fixture is used to open the bottle cap of the second reagent storage bottle so that the pipetting assembly 13 transfers the hemolytic agent in the second reagent storage bottle to the detection pool 112 through the second pipette head 131. The moving fixture is also used to close the bottle cap of the second reagent storage bottle. The detection pool 112 stores the second sample liquid treated with the hemolytic agent. The impedance detection component of the detection assembly is used to perform impedance counting on the second sample liquid in the detection pool 112 to obtain a second detection result of white blood cells, and / or the optical detection component of the detection assembly is used to perform optical measurement on the second sample liquid in the detection pool 112 to obtain a third detection result of hemoglobin based on the above-mentioned optical background value. It can be understood that when the detection pool 112 includes two or more detection pools, there is no need to transfer and clean the waste liquid after obtaining the first test result. The pipetting component 13 can directly transfer the sample that has been diluted once in the dilution pool 113 to another detection pool 112 for testing the second item, which is not specifically limited here.

[0060] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A POCT blood cell analyzer, characterized in that: include: The housing is provided with a detection position, the detection position is used to place a detection box, and the detection box is loaded with a sample; A reagent storage component, disposed in the housing, for storing detection reagents; A liquid transfer assembly, disposed in the housing, for adding the detection reagent to the detection box so that the sample reacts with the detection reagent and obtains a sample liquid to be tested; The detection component is arranged on the housing and is used for performing a counting test on the sample liquid to be tested in the detection box to obtain a test result.

2. The POCT blood cell analyzer according to claim 1, characterized in that: The reagent storage assembly includes a first reagent storage bottle and a second reagent storage bottle, the first reagent storage bottle is used to store a diluent, the second reagent storage bottle is used to store a hemolytic agent, and the pipetting assembly is used to add at least one of the diluent and the hemolytic agent to the detection box.

3. The POCT blood cell analyzer according to claim 2, characterized in that: The detection box comprises a sample pool, a detection pool and a dilution pool, the sample pool is used to carry the sample, and the detection pool and the dilution pool are arranged on one side or two opposite sides of the sample pool; The pipetting component is used to add the diluent to the dilution pool and the detection pool respectively. The pipetting component is also used to transfer the sample from the sample pool to the dilution pool and transfer the liquid from the dilution pool to the detection pool, so that the detection pool stores a first sample liquid for detecting a first item. The pipetting component is also used to add the hemolytic agent to the dilution pool, so that the dilution pool stores a second sample liquid for detecting a second item.

4. The POCT blood cell analyzer according to claim 2, characterized in that: The detection box includes a sample pool and a detection pool, the sample pool is used to hold the sample, the detection pool is arranged on one side of the sample pool, the pipetting component is used to add the diluent and the sample to the detection pool respectively, or the pipetting component is used to add the diluent, the sample and the hemolytic agent to the detection pool respectively to obtain the sample liquid to be tested.

5. The POCT blood cell analyzer according to claim 3 or 4, characterized in that: The number of the detection pool is at least one.

6. The POCT blood cell analyzer according to claim 2, characterized in that: The reagent storage assembly also includes a movable clamp, which is arranged on the housing. The first reagent storage bottle and the second reagent storage bottle include a bottle body and a bottle cap. The bottle cap is used to seal the detection reagent stored in the bottle body. The movable clamp is used to open or close the bottle caps of the first reagent storage bottle and the second reagent storage bottle.

7. The POCT blood cell analyzer according to claim 1, characterized in that: The POCT blood cell analyzer also includes a pipette tip storage assembly, which is arranged in the housing and is used to store multiple pipette tips. The pipette tip storage assembly is used to move to the pipette tip storage assembly and plug in the pipette tip to perform liquid transfer operations through the pipette tip.

8. The POCT blood cell analyzer according to claim 7, characterized in that: The pipette tip storage assembly includes a first storage position and a second storage position, wherein at least one first pipette tip is placed in the first storage position, and at least one second pipette tip is placed in the second storage position, wherein the pipetting assembly is used to insert the first pipette tip from the first storage position and absorb the diluent of the reagent storage assembly through the first pipette tip, and the pipetting assembly is also used to insert the second pipette tip from the second storage position and absorb the hemolytic agent of the reagent storage assembly through the second pipette tip.

9. The POCT blood cell analyzer according to claim 1, characterized in that: The POCT blood cell analyzer also includes a remaining amount sensor, which is arranged in the reagent storage component. The remaining amount sensor is used to detect the reagent remaining amount in the reagent storage component to generate a warning message when the reagent remaining amount is lower than a preset threshold.

10. The POCT blood cell analyzer according to claim 1, characterized in that: The detection box comprises a sample pool, a detection pool and a dilution pool, and the sample pool, the detection pool and the dilution pool are arranged in a linear direction.

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