Composite pathogen cracking device

Abstract

The utility model provides a combined type pathogen cracking device which comprises a device support, a chip bearing platform is erected on the device support, the top face of the chip bearing platform is used for fixing a pathogen cracking and nucleic acid extraction micro-fluidic chip, and a magnetic rotating assembly is further arranged on the chip bearing platform. The magnetic rotating assembly comprises a rotating motor and a rotating magnet connected to a rotating shaft of the rotating motor, a heating piece is arranged on the chip bearing platform, and the heating piece is used for heating a sample liquid pipe in the pathogen splitting decomposition and nucleic acid extraction micro-fluidic chip arranged on the chip bearing platform. According to the utility model, a thermal cracking mode is combined while mechanical cracking of pathogens is realized, so that the cracking efficiency of the pathogens can be obviously improved, and the requirements of instant field detection and inspection can be met.

Description

Technical Field

[0001] The utility model belongs to the technical field of microfluidics, and in particular relates to a composite pathogen lysis device. Background Art

[0002] Microfluidics is a technology that controls fluids at a microscale. Its development originated from the field of microelectromechanical systems (MEMS) in the 1980s. Microfluidics is widely used in life sciences, medical diagnosis, environmental monitoring and other fields due to its high degree of integration, efficient and precise fluid control, and low sample consumption. Especially in the medical field, microfluidics provides convenient, fast and efficient solutions for key steps such as pathogen detection and nucleic acid extraction.

[0003] Pathogen lysis is a key step to destroy the nucleic acid packaging structure in the sample and release the nucleic acid for further analysis. For bacteria and fungi, traditional pathogen lysis methods include chemical, mechanical and thermal lysis. Chemical lysis refers to the use of high-salt reagents, alkaline environment reagents, lysozyme and other chemical reagents to destroy the cell membrane or bacterial wall, but this method is time-consuming, inefficient, and may contain conditions that inhibit downstream nucleic acid analysis; mechanical methods use mechanical forces, such as shear force, to destroy the structure of cells or bacteria and release nucleic acids, which is efficient and fast. There are some existing mechanical lysis instruments, such as homogenizers, which can achieve efficient microbial lysis, but the instrument is bulky and not conducive to portable on-site detection and analysis. Thermal lysis is to destroy the structure of bacteria or cells at high temperature and release nucleic acids, but the thermal lysis method is inefficient, and long-term high temperature will destroy the target substance, resulting in limited application scenarios. Therefore, in the real-time on-site detection and analysis, there is currently no efficient nucleic acid lysis method for the lysis and release of pathogenic microorganisms. Nucleic acid extraction is to extract nucleic acids from the lysed sample for subsequent molecular biological analysis. Traditional nucleic acid extraction methods include phenol / chloroform method, centrifugal column method and magnetic bead method, but these methods have problems such as cumbersome operation, large sample loss and low extraction efficiency. Some automated nucleic acid extraction devices, such as robotic arm-based nucleic acid extractors, have problems such as large size, open reagents, and easy contamination, which are not suitable for instant on-site testing. Compared with an ideal bedside detection device, it is particularly necessary to have a device that can efficiently achieve pathogen lysis. Utility Model Content

[0004] The utility model provides a composite pathogen lysis device, which can solve the technical problem that the pathogen lysis efficiency of the device adopting a single lysis mode in the prior art is low and cannot meet the demand of instant on-site detection and inspection.

[0005] In order to solve the above problems, the utility model provides a composite pathogen lysis device, including a device bracket, a chip carrying platform is mounted on the device bracket, the top surface of the chip carrying platform is used to fix the pathogen lysis and nucleic acid extraction microfluidic chip, and a magnetic rotating component is also provided on the chip carrying platform. The magnetic rotating component includes a rotating motor and a rotating magnet connected to the rotating shaft of the rotating motor. A heating element is provided on the chip carrying platform, and the heating element is used to heat the sample liquid tube in the pathogen lysis and nucleic acid extraction microfluidic chip placed on the chip carrying platform.

[0006] In some embodiments, the rotating magnet is offset to one side of the rotating shaft of the rotating motor, so that the rotating motor can intermittently form magnetic adsorption on the magnetically driven stirring member in the sample liquid tube when rotating.

[0007] In some embodiments, the magnetic rotating assembly further includes a mass balancing block, which is a non-magnetic part and is symmetrically located on opposite sides of the rotating shaft of the rotating motor with respect to the rotating magnet, and the mass of the mass balancing block is equal to that of the rotating magnet.

[0008] In some embodiments, a rotating block is connected to the rotating shaft of the rotating motor, and the rotating block has two accommodating grooves that are symmetrically arranged on the left and right. The mass balance block and the rotating magnet are respectively assembled in one accommodating groove.

[0009] In some embodiments, an assembly groove is formed on the chip carrying platform, and the heating element is assembled in the assembly groove.

[0010] In some embodiments, two opposite sides of the assembly groove have slide grooves respectively, and two chip clamps are slidably connected in the slide grooves, and the two chip clamps can clamp the bottom end of the microfluidic chip between the two.

[0011] In some embodiments, a heat conductive member is further provided on the top surface of the heating member, and the heat conductive member is a sheet structure with a notch on one side, and the sheet structure is wrapped around the outer wall of the sample liquid tube, and its height is not lower than the height of the fluid in the sample liquid tube.

[0012] In some embodiments, a digital display temperature control panel is further provided on the chip carrying platform.

[0013] The utility model provides a composite pathogen lysis device, which has the following beneficial effects: the magnetic rotating component can drive the magnetic force driven stirring element in the sample liquid tube of the microfluidic chip to rotate, thereby achieving the grinding and wall breaking of the pathogens in the sample liquid tube, and the heating element can heat and incubate the pathogens in the sample liquid tube. In this way, while achieving mechanical lysis of the pathogens, it also combines the thermal lysis method, which can significantly improve the lysis efficiency of the pathogens and meet the needs of immediate on-site detection and inspection. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the implementation or the prior art description. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0015] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0016] Figure 1 The schematic diagram is a principle diagram of mechanical lysis using the pathogen lysis and nucleic acid extraction microfluidic chip of the utility model, wherein;

[0017] Figure 2 The utility model discloses a composite pathogen lysis device which is used in conjunction with the pathogen lysis and nucleic acid extraction microfluidic chip of the utility model.

[0018] The reference numerals are:

[0019] 1. Microfluidic chip for pathogen lysis and nucleic acid extraction; 131. Sample liquid tube; 17. Grinding lysis solution (containing microbeads for grinding); 16. Magnetic-driven stirring element; 61. Chip carrying platform; 621. Heat conducting element; 622. Heating element; 631. Rotating magnet; 632. Mass balance block; 633. Rotating motor; 634. Rotating block; 64. Chip fixture; 65. Device bracket; 66. Digital display temperature control board. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means a limitation on the utility model and its application or use. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0021] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0022] It should be understood that the term "and / or" used in this article is only a description of the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0023] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps described in these embodiments do not limit the scope of the utility model. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be regarded as a part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once a certain item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0024] In the description of the present utility model, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction, and therefore cannot be understood as limiting the scope of protection of the present utility model; the directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.

[0025] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0026] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. If not otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the utility model.

[0027] See also Figure 1 and Figure 2 As shown, the utility model also provides a composite pathogen lysis device for use with the pathogen lysis and nucleic acid extraction microfluidic chip of the utility model, the lysis device includes a device bracket 65, a chip carrying platform 61 is mounted on the device bracket 65, the top surface of the chip carrying platform 61 is used to fix the pathogen lysis and nucleic acid extraction microfluidic chip, the chip carrying platform 61 is also provided with a magnetic rotating component, the magnetic rotating component includes a rotating motor 633 and a rotating magnet 631 connected to the rotating shaft of the rotating motor 633, a heating element 622 is provided on the chip carrying platform 61, the heating element 622 is used to heat the sample liquid tube 131 in the pathogen lysis and nucleic acid extraction microfluidic chip placed on the chip carrying platform 61, the aforementioned heating element 622 can be a heating film, and the heating film is flat on the chip carrying platform 61.

[0028] In this technical solution, the magnetic rotating component can drive the magnetically driven stirring element 16 in the sample liquid tube 131 of the microfluidic chip to rotate, thereby achieving the grinding and breaking of the pathogens in the sample liquid tube 131 (specifically by grinding the microbeads in the lysis solution 17). At the same time, the heating element 622 can heat and incubate the pathogens in the sample liquid tube 131. In this way, while achieving mechanical lysis of the pathogens, it also combines the thermal lysis method, which can significantly improve the lysis efficiency of the pathogens and meet the needs of immediate on-site detection and inspection.

[0029] In some embodiments, see again Figure 1 As shown, in a preferred embodiment, the aforementioned rotating magnet 631 is offset to one side of the rotating shaft of the rotating motor 633, so that when the rotating motor 633 rotates, it can intermittently form magnetic adsorption on the magnetically driven stirring member 16 in the sample liquid tube 131, which is beneficial to increase the irregularity of the rotation of the magnetically driven stirring member 16, thereby increasing its stirring intensity and improving the grinding and wall breaking effect.

[0030] The diameter of the aforementioned rotating magnet 631 is D2, which should generally be larger than the maximum size of the magnetically driven stirring member 16, and the distance L between the rotating magnet 631 and the bottom end surface of the sample liquid tube 131 is as small as possible to ensure that the follow-up driving effect of magnetic adsorption is more effective. In a preferred embodiment, the rotating magnet 631 is coaxially arranged with the sample liquid tube 131.

[0031] In order to prevent the biased rotating magnet 631 from causing mass imbalance during the rotation of the rotating motor 633, thereby causing the generation of operating noise, in a preferred embodiment, the magnetic rotating assembly also includes a mass balancing block 632, which is a non-magnetic part (that is, it does not generate magnetic force). It is symmetrical with the rotating magnet 631 and is located on opposite sides of the rotating shaft of the rotating motor 633. At this time, the corresponding mass balancing block 632 is equal to the mass of the rotating magnet 631, thereby achieving force balance during the rotation of the rotating motor 633 and reducing the generation of noise during operation.

[0032] In a specific embodiment, a rotating block 634 is connected to the rotating shaft of the rotating motor 633. The rotating block 634 has two symmetrically arranged receiving grooves (not marked in the figure), and the mass balance block 632 and the rotating magnet 631 are respectively assembled in one receiving groove.

[0033] In a specific embodiment, an assembly groove is formed on the chip supporting platform 61, and the heating element 622 is assembled in the assembly groove. In a preferred embodiment, there are slide grooves on the opposite sides of the assembly groove respectively, and two chip clamps 64 are slidably connected in the slide grooves, and the two chip clamps 64 can clamp the bottom end of the microfluidic chip between the two.

[0034] In order to improve the heating and incubation efficiency of the heating element 622 on the sample liquid in the sample liquid tube 131, a heat conductive element 621 is further provided on the top surface of the heating element 622. The heat conductive element 621 is a sheet structure with a notch on one side. The sheet structure is wrapped around the outer wall of the sample liquid tube 131, and its height is not lower than the fluid height in the sample liquid tube 131.

[0035] In another feasible embodiment, a digital temperature control panel 66 is further provided on the chip carrying platform 61 , which allows an operator to directly adjust the heating temperature of the heating element 622 (generally around 65° C.).

[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0037] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A composite pathogen lysis device, characterized in that: The invention comprises a device support (65), wherein a chip carrying platform (61) is mounted on the device support (65), wherein the top surface of the chip carrying platform (61) is used to fix a pathogen lysis and nucleic acid extraction microfluidic chip (1), wherein a magnetic rotating component is also provided on the chip carrying platform (61), wherein the magnetic rotating component comprises a rotating motor (633) and a rotating magnet (631) connected to a rotating shaft of the rotating motor (633), and wherein a heating element (622) is provided on the chip carrying platform (61), wherein the heating element (622) is used to heat a sample liquid tube (131) in the pathogen lysis and nucleic acid extraction microfluidic chip (1) placed on the chip carrying platform (61).

2. The composite pathogen lysis device according to claim 1, characterized in that: The rotating magnet (631) is offset to one side of the rotating shaft of the rotating motor (633), so that when the rotating motor (633) rotates, it can intermittently form magnetic attraction on the magnetically driven stirring member (16) in the sample liquid tube (131).

3. The composite pathogen lysis device according to claim 1, characterized in that: The magnetic rotating assembly further comprises a mass balancing block (632), which is a non-magnetic part and is symmetrically located with the rotating magnet (631) on opposite sides of the rotating shaft of the rotating motor (633). The mass of the mass balancing block (632) is equal to that of the rotating magnet (631).

4. The composite pathogen lysis device according to claim 3, characterized in that: A rotating block (634) is connected to the rotating shaft of the rotating motor (633), and the rotating block (634) has two accommodating grooves that are symmetrically arranged on the left and right. The mass balance block (632) and the rotating magnet (631) are respectively assembled in one accommodating groove.

5. The composite pathogen lysis device according to claim 1, characterized in that: An assembly groove is formed on the chip carrying platform (61), and the heating element (622) is assembled in the assembly groove.

6. The composite pathogen lysis device according to claim 5, characterized in that: The two opposite sides of the assembly groove are respectively provided with slide grooves, and two chip clamps (64) are slidably connected in the slide grooves, and the two chip clamps (64) can clamp the bottom end of the microfluidic chip between them.

7. The composite pathogen lysis device according to claim 1, characterized in that: A heat conducting element (621) is also provided on the top surface of the heating element (622). The heat conducting element (621) is a sheet structure having a notch on one side. The sheet structure is wrapped around the outer wall of the sample liquid tube (131), and its height is not lower than the height of the fluid in the sample liquid tube (131).

8. The composite pathogen lysis device according to claim 1, characterized in that: A digital display temperature control panel (66) is also provided on the chip carrying platform (61).

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