NK cell incubator
By designing the sealing gasket, placing plates, guide grooves, guide columns and roller structures in the NK cell incubator, the problem of the culture plate being easily tilted and causing the culture liquid to be dissipated, achieving a more stable and convenient culture plate operation.
Patent Information
- Application Number
- CN202420515777.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-18
- Publication Date
- 2025-02-25
- Estimated Expiration
- 2034-03-18
AI Technical Summary
During the NK cell culture process, the user needs to hold a multi-porous culture plate in the incubator, which can easily lead to the inclination of the culture plate, causing the culture liquid to dissipate, causing inconvenience to the culture.
An NK cell incubator is designed, including a sealing gasket, multiple placement plates, a first guide groove, a guide column, a second guide groove and a roller structure. Through the combination of these structures, the placement plate can slide stably to avoid tilt and liquid. shed.
This design not only facilitates the replacement of the placed plate and the pick-up and placement of the culture plate, but also increases the stability and convenience of pick-up and placement, reduces the risk of spilling culture liquid, and improves the user's operation and control ability.
Smart Images
Figure CN222524560U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of NK cell culture, in particular to a NK cell culture box. Background Art
[0002] NK cells (natural killer cells) are an important part of our immune system. They can recognize and kill virus-infected cells and certain types of cancer cells. In the laboratory, scientists often need to culture NK cells for various research.
[0003] In the process of NK cell culture, first, blood or bone marrow samples need to be separated to obtain NK cell precursor cells, and then the purified NK cell precursor cells are inoculated into a culture medium containing growth factors and nutrients, and finally placed in a NK cell incubator for culture.
[0004] During the culture, the culture medium (purified NK cell precursor cells are inoculated into a culture medium containing growth factors and nutrients) is generally added to the multi-well culture plate, and then the culture plate is placed in the NK cell incubator, and a certain temperature and CO are set. 2 concentration, and then culture it.
[0005] However, when using it, the user needs to hold the multi-well culture plate and place it in the incubator. During this process, the user needs to put his hand deep into the incubator. This posture can easily cause the culture plate to tilt, resulting in the spillage of the culture solution, which brings inconvenience to the culture. Utility Model Content
[0006] In view of the deficiencies in the prior art, the utility model provides a NK cell culture box, the purpose of which is to solve the above problems.
[0007] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0008] A NK cell culture box, comprising a box body and a box door, and also comprising:
[0009] A sealing gasket, which is arranged between the box body and the box door;
[0010] A plurality of placement plates are arranged inside the box;
[0011] A plurality of first guide grooves are provided on the front and rear sides of the placement plate;
[0012] A plurality of guide posts, which are fixed to the front and rear sides of the inner wall of the box body and extend to the inside of the first guide groove;
[0013] A plurality of second guide grooves are provided on the front and rear sides of the inner wall of the box body;
[0014] A plurality of roller structures are arranged on the front and rear sides of the placement plate, extend to the inside of the second guide groove, and are located on a side away from the box door.
[0015] Preferably, a handle is fixed to the bottom of the placement plate, and the handle is close to the box door.
[0016] Preferably, the first guide groove comprises:
[0017] A guide groove is transversely opened on the side of the placement plate;
[0018] The guide post slides inside the guide groove;
[0019] The second guide groove comprises:
[0020] A transverse groove is provided on the inner wall of the box body;
[0021] The roller structure slides in the transverse groove.
[0022] Preferably, the roller structure comprises:
[0023] A fixed shaft, which is fixed to the side of the placement plate;
[0024] The rotating wheel is rotatably sleeved on the outside of the fixed shaft through a bearing and is located inside the transverse groove.
[0025] Preferably, the first guide groove further includes:
[0026] A through slot is vertically opened on the side of the placement plate and communicates with the side of the guide slot away from the box door;
[0027] The second guide groove also includes:
[0028] The vertical groove is formed on the inner wall of the box body and is connected with one end of the horizontal groove close to the box door.
[0029] Preferably, a first limit block is fixed inside the guide groove, and the first limit block is away from the box door.
[0030] Preferably, a second limit block is fixed inside the transverse groove, and the second limit block is close to the box door.
[0031] Compared with the prior art, the utility model has the following beneficial effects:
[0032] The utility model provides an NK cell culture box, which is not only convenient for changing the layers of the placement plates, but also convenient for users to take and place the culture plates, is not prone to skewing, and is better controlled by users, thereby increasing the stability and convenience of taking and placing, and is more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS
[0033] Figure 1 This is a schematic diagram of the structure of a NK cell culture box described in the utility model;
[0034] Figure 2 for Figure 1 A schematic diagram of the structure enlargement in the middle;
[0035] Figure 3 It is an enlarged schematic diagram of the structure of the placement plate described in the present invention.
[0036] In the figure: 10 box body, 11 box door, 20 sealing gasket, 30 placement plate, 31 handle, 40 first guide groove, 41 guide groove, 42 through groove, 43 first limit block, 50 guide column, 60 second guide groove, 61 horizontal groove, 62 vertical groove, 63 second limit block, 70 roller structure, 71 fixed shaft, 72 rotating wheel, 73 bearing. DETAILED DESCRIPTION
[0037] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0038] A NK cell culture box, comprising a box body 10 and a box door 11, wherein the box body 10 and the box door 11 represent the structure of an existing NK cell culture box, wherein how to control the temperature and how to control the CO 2 The concentration can be adjusted using existing methods, which will not be elaborated in this application.
[0039] In the examples of this application, please refer to Figure 1 , 2 3. A sealing gasket 20 is provided between the box body 10 and the box door 11. A plurality of placement plates 30 are provided inside the box body 10. A plurality of through holes are provided in the middle of the placement plates 30. First guide grooves 40 are provided on both the front and rear sides of the placement plates 30. A plurality of guide posts 50 are fixed on both the front and rear sides of the inner wall of the box body 10. The guide posts 50 extend into the first guide grooves 40. Specifically, the first guide grooves 40 include guide grooves 41 transversely provided on the side of the placement plates 30. The guide posts 50 slide inside the guide grooves 41.
[0040] The user can first pull out the placement plate 30, then place the culture plate on the placement plate 30, and then slowly slide the placement plate 30 in. The operation is simple and no longer requires the user to put both hands in. Therefore, the user can better control the stability of both hands when taking and placing, and the culture solution is not easily spilled, which brings convenience to the user.
[0041] For further information, see Figure 1 , 2and 3. A plurality of second guide grooves 60 are provided on the front and rear sides of the inner wall of the box body 10, and a roller structure 70 extending into the second guide groove 60 is provided on the front and rear sides of the placement plate 30, and the roller structure 70 is located on the side of the placement plate 30 away from the box door 11, wherein the second guide groove 60 includes a transverse groove 61 provided on the inner wall of the box body 10, and the roller structure 70 slides in the transverse groove 61. In the process of the placement plate 30 sliding in and out, the roller structure 70 slides in the transverse groove 61, and the guide column 50 slides inside the guide groove 41, and they cooperate with each other to stabilize the sliding of the placement plate 30.
[0042] Meanwhile, a handle 31 is fixed to the bottom of the placement plate 30 , and the handle 31 is close to the box door 11 , so that the user can pull the placement plate 30 .
[0043] For further examples, see Figure 1 , 2 And 3, in order to facilitate the adjustment of the position of the placement plate 30, the first guide groove 40 also includes a through groove 42 vertically opened on the side of the placement plate 30, the through groove 42 is connected to the side of the guide groove 41 away from the box door 11, and the second guide groove 60 also includes a vertical groove 62 opened on the inner wall of the box body 10, and the vertical groove 62 is connected to the end of the horizontal groove 61 close to the box door 11. When the position needs to be changed, the placement plate 30 is pulled out to the maximum stroke, and then the placement plate 30 is moved up or down, so that the corresponding guide column 50 is drawn through the through groove 42, and then the new guide column 50 slides in, and at the same time, the roller structure 70 slides into the new horizontal groove 61 through the vertical groove 62, and then the placement plate 30 is pushed in to complete the position adjustment.
[0044] In order to avoid directly extending the placement plate 30 to its maximum stroke during normal use, a first limit block 43 is fixed inside the guide groove 41, and the first limit block 43 is away from the box door 11. A second limit block 63 is fixed inside the transverse groove 61, and the second limit block 63 is close to the box door 11. When the placement plate 30 is pulled out, the guide column 50 and the roller structure 70 will be blocked by the first limit block 43 and the second limit block 63, and thus cannot be pulled further, thereby avoiding accidentally pulling the placement plate 30 over the position. When the position needs to be changed, the placement plate 30 is moved so that the guide column 50 and the roller structure 70 bypass the first limit block 43 and the second limit block 63, and then the above-mentioned position change operation is continued.
[0045] In a further embodiment, the roller structure 70 includes a fixed shaft 71 fixed to the side of the placement plate 30, and the outside of the fixed shaft 71 is rotatably connected to a rotating wheel 72 through a bearing 73. The rotating wheel 72 is located inside the transverse groove 61. During the sliding of the placement plate 30, the sliding can be facilitated by the rolling of the rotating wheel 72.
[0046] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.
[0047] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A NK cell culture box, comprising a box body and a box door, characterized in that: Also includes: A sealing gasket, which is arranged between the box body and the box door; A plurality of placement plates are arranged inside the box; A plurality of first guide grooves are provided on the front and rear sides of the placement plate; A plurality of guide posts, which are fixed to the front and rear sides of the inner wall of the box body and extend to the inside of the first guide groove; A plurality of second guide grooves are provided on the front and rear sides of the inner wall of the box body; A plurality of roller structures are arranged on the front and rear sides of the placement plate, extend to the inside of the second guide groove, and are located on a side away from the box door.
2. A NK cell culture box according to claim 1, characterized in that: A handle is fixed at the bottom of the placement plate, and the handle is close to the box door.
3. The NK cell culture box according to claim 1, characterized in that: The first guide groove comprises: A guide groove is transversely opened on the side of the placement plate; The guide post slides inside the guide groove; The second guide groove comprises: A transverse groove is provided on the inner wall of the box body; The roller structure slides in the transverse groove.
4. A NK cell culture box according to claim 3, characterized in that: The roller structure comprises: A fixed shaft, which is fixed to the side of the placement plate; The rotating wheel is rotatably sleeved on the outside of the fixed shaft through a bearing and is located inside the transverse groove.
5. The NK cell culture box according to claim 3, characterized in that: The first guide groove further comprises: A through slot is vertically opened on the side of the placement plate and communicates with the side of the guide slot away from the box door; The second guide groove also includes: The vertical groove is formed on the inner wall of the box body and is connected with one end of the horizontal groove close to the box door.
6. The NK cell culture box according to claim 5, characterized in that: A first limiting block is fixed inside the guide groove, and the first limiting block is away from the box door.
7. The NK cell culture box according to claim 5, characterized in that: A second limiting block is fixed inside the transverse groove, and the second limiting block is close to the box door.