Chromatographic column for cell culture plate

Abstract

The utility model provides a chromatographic column for a cell culture plate, which relates to the technical field of chemical experiment equipment and comprises a culture plate base, culture plate tube banks are arranged at the top of the culture plate base and are distributed in a linear array manner; a mounting frame body is arranged above the culture plate base; eight chromatographic column main bodies are arranged on the mounting frame body, the mounting frame body is controlled to drive the chromatographic column main bodies to slide left and right by utilizing the sliding fit of the limiting sleeves and the limiting round rods, and the use of all culture plate racking pipes in the equipment is met by adjusting the positions of the chromatographic column main bodies, so that the manufacturing cost is effectively reduced, and the utilization rate and the applicability are greatly improved; the 96-hole cell culture plate solves the problems that the number of configured chromatographic columns of an existing 96-hole cell culture plate is large, accurate control over each chromatographic column is difficult to achieve, the equipment manufacturing cost is high, opening and closing of the multiple chromatographic columns are inconvenient, the action process is tedious, and the experiment efficiency is low.

Description

Technical Field

[0001] The utility model relates to the technical field of chemical experimental equipment, in particular to a chromatography column for a cell culture plate. Background Art

[0002] Cell culture plates are one of the commonly used experimental equipment in laboratories, suitable for experiments such as exploring and optimizing initial experimental conditions in cells and culture. Among them, column chromatography columns suitable for cell culture plates can separate and purify biotechnology products.

[0003] For example, application number: CN201320507899.7 relates to a chromatography column for a cell culture plate, including a frame and a plurality of long tubes. The frame includes a column and an upper bracket and a lower bracket fixed to the column; the long tube is vertically clamped on the upper and lower brackets; a top cover is provided on the top of the long tube; a liquid inlet is provided on the upper part of the top cover, and a plurality of liquid dispensing tubes connected to the liquid inlet and corresponding to the long tube are provided on the lower part; the liquid dispensing tubes are tightly embedded in the long tube; a filter is provided at the lower end of each long tube, and a liquid outlet tube is provided; a base is provided under the lower bracket. The utility model has a reasonable structure, is practical and convenient, and can evenly distribute the chromatographic mobile phase to each column and maintain a consistent flow rate, so that the components contained in the sample can be separated and collected into the plate holes of the cell culture plate in a basically consistent time, simplifying the operation.

[0004] Based on the search of the above patents and combined with the equipment in the prior art, it was found that the existing 96-well cell culture plates are equipped with a large number of chromatography columns, making it difficult to achieve precise control of each chromatography column. Not only is the equipment manufacturing cost high, but it is also inconvenient to open and close multiple chromatography columns, and the operation process is cumbersome, resulting in low experimental efficiency. Utility Model Content

[0005] The disclosed embodiment relates to a chromatography column for a cell culture plate, which utilizes the sliding cooperation of a limiting sleeve and a limiting round rod to control the mounting frame to drive the chromatography column body to slide left and right. By adjusting the position of the chromatography column body, the use of all culture plate arrangement tubes in the device can be met, thereby effectively reducing the manufacturing cost and greatly improving the utilization rate and applicability.

[0006] In a first aspect of the present disclosure, a chromatography column for a cell culture plate is provided, which specifically includes: a culture plate base, a culture plate pipe is provided on the top of the culture plate base, and the culture plate pipe is distributed in a linear array; a mounting frame is provided above the culture plate base; eight chromatography column bodies are provided on the mounting frame, and the eight chromatography column bodies are distributed in a linear array; a liquid outlet pipe is provided at the bottom of the chromatography column body.

[0007] In at least some embodiments, the culture plate base is a rectangular cavity structure, and a partition is provided in the cavity of the culture plate base, the partition is distributed longitudinally and transversely, and a limiting groove is provided on the transverse partition, and a first compression spring is provided on the front and rear sides of the cavity of the culture plate base, and the front and rear first compression springs are distributed in a symmetrical manner.

[0008] In at least some embodiments, support frames are provided on the left and right sides of the top of the culture plate base, and the two support frames are symmetrically distributed, and two limiting round rods are provided between the two culture plate bases, and the two limiting round rods are distributed in a front-to-back parallel manner.

[0009] In at least some embodiments, the culture plate tube arrangement is composed of eight column tubes, and a connecting block is provided between every two adjacent column tubes, and the culture plate tube arrangement is slidably connected to the limiting groove of the culture plate base through the connecting block, and a lifting plate is provided on the front and rear sides of the culture plate tube arrangement, and a first compression spring is supported at the bottom of the lifting plate, and a docking sleeve is provided on the top of the lifting plate.

[0010] In at least some embodiments, the mounting frame includes a connecting splint, a connecting rod, a fixing seat and a limiting sleeve. The connecting splints are divided into two groups and are symmetrically distributed in an upper and lower manner. The chromatography column body is clamped between the upper and lower groups of connecting splints and controlled by locking bolts. Four connecting rods are arranged between the upper and lower groups of connecting splints, and every two connecting rods form a group and are symmetrically distributed in a left-right manner. A fixing seat is arranged in the middle position of each group of connecting rods, and limiting sleeves are arranged on the outer sides of the two fixing seats, and the limiting sleeves are slidably mounted on the limiting round rods.

[0011] In at least some embodiments, a connector is provided at the bottom end of the chromatography column body, a sliding sleeve is provided at the center of the connector, and the liquid outlet pipe is slidably connected to the connector through the sliding sleeve.

[0012] In at least some embodiments, a connecting plate is provided between the eight liquid outlet pipes, docking posts are provided at the front and rear ends of the connecting plate, and the docking posts match the docking sleeve, a second compression spring is provided on the liquid outlet pipe, and the second compression spring is supported between the connecting plate and the connector.

[0013] In at least some embodiments, a sealing ring is provided at the top of the liquid outlet pipe, and the sealing ring matches the top of the sliding sleeve, and a liquid inlet is provided at the top of the side wall of the liquid outlet pipe.

[0014] The utility model provides a chromatography column for a cell culture plate, which has the following beneficial effects:

[0015] In the utility model, the lifting and pressing plates of the culture plate tubes cooperate with the first compression spring, and the front and rear lifting and pressing plates can be manually pressed to make the culture plate tubes slide downward along the limiting groove, so that the docking sleeve can be detached from the bottom end of the docking column. At the same time, the limiting sleeve and the limiting round rod can be slidably cooperated to control the mounting frame to drive the chromatography column body to slide left and right. By adjusting the position of the chromatography column body, the use of all culture plate tubes in the device can be met, which effectively reduces the manufacturing cost and greatly improves the utilization rate and applicability.

[0016] In addition, by lifting the lifting plate of the culture plate tube upwards and utilizing the cooperation between the docking sleeve and the docking column, the connecting plate can drive the liquid outlet tube to slide upwards. When the liquid inlet is separated from the sliding sleeve, the chromatography liquid in the chromatography column body drips into the culture plate tube through the liquid inlet and the liquid outlet tube. The operation process is simple and can simultaneously control the opening and closing of eight chromatography column bodies, eliminating the trouble of step-by-step operation and effectively improving the utilization efficiency of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solution of the embodiment of the utility model, the drawings of the embodiment will be briefly introduced below.

[0018] The drawings described below only relate to some embodiments of the present invention, but are not intended to limit the present invention.

[0019] In the attached picture:

[0020] Figure 1 The overall axial viewing angle structure schematic diagram of the present application is shown;

[0021] Figure 2 A schematic diagram of the culture plate base structure of the present application is shown;

[0022] Figure 3 This application shows Figure 2 A schematic diagram of the enlarged structure of the middle part A;

[0023] Figure 4 A schematic diagram of the culture plate pipe arrangement structure of the present application is shown;

[0024] Figure 5 The schematic diagram of the structure of the mounting frame, chromatography column body and liquid outlet pipe of the present application is shown;

[0025] Figure 6 A schematic diagram of the cross-sectional structure of a portion of the chromatography column body of the present application is shown.

[0026] List of reference numerals:

[0027] 1. Culture plate base; 101. Partition plate; 102. Limiting groove; 103. First compression spring; 104. Support frame; 105. Limiting round rod;

[0028] 2. Culture plate pipe arrangement; 201. Connecting block; 202. Lifting and pressing plate; 203. Docking sleeve;

[0029] 3. Mounting frame; 301. Connecting splint; 302. Connecting rod; 303. Fixing seat; 304. Limiting sleeve;

[0030] 4. Chromatographic column body; 401. Connector; 402. Sliding sleeve;

[0031] 5. Liquid outlet pipe; 501. Connecting plate; 502. Docking column; 503. Second compression spring; 504. Sealing ring; 505. Liquid inlet. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution of the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings of the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. Based on the described embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] Example 1: Please refer to Figures 1 to 6 :

[0034] The utility model proposes a chromatography column for a cell culture plate, comprising: a culture plate base 1, a culture plate row pipe 2 is arranged on the top of the culture plate base 1, and the culture plate row pipe 2 is distributed in a linear array; a mounting frame 3 is arranged above the culture plate base 1; eight chromatography column bodies 4 are arranged on the mounting frame 3, and the eight chromatography column bodies 4 are distributed in a linear array; a liquid outlet pipe 5 is arranged at the bottom of the chromatography column body 4.

[0035] In the present disclosure, Figures 2 to 5 As shown, the culture plate base 1 is a rectangular cavity structure, and a partition 101 is provided in the cavity of the culture plate base 1, the partition 101 is distributed longitudinally and transversely, and a limiting groove 102 is provided on the transverse partition 101, and a first compression spring 103 is provided on the front and rear sides of the cavity of the culture plate base 1, and the front and rear first compression springs 103 are distributed in a symmetrical manner;

[0036] Support frames 104 are provided on the left and right sides of the top of the culture plate base 1, and the two support frames 104 are symmetrically distributed. Two limiting round rods 105 are provided between the two culture plate bases 1, and the two limiting round rods 105 are distributed in a front-to-back parallel manner.

[0037] The culture plate tube arrangement 2 is composed of eight column tubes, and a connecting block 201 is provided between every two adjacent column tubes, and the culture plate tube arrangement 2 is slidably connected to the limiting groove 102 of the culture plate base 1 through the connecting block 201, and a lifting plate 202 is provided on the front and rear sides of the culture plate tube arrangement 2, and a first compression spring 103 is supported at the bottom of the lifting plate 202, and a docking sleeve 203 is provided on the top of the lifting plate 202;

[0038] The mounting frame 3 includes a connecting splint 301, a connecting rod 302, a fixing seat 303 and a limiting sleeve 304. The connecting splint 301 is composed of two groups and is distributed in an upper and lower symmetrical manner. The chromatography column body 4 is clamped between the upper and lower two groups of connecting splints 301 and is controlled by a locking bolt. Four connecting rods 302 are arranged between the upper and lower two groups of connecting splints 301, and every two connecting rods 302 form a group and are distributed in a left-right symmetrical manner. A fixing seat 303 is arranged in the middle of each group of connecting rods 302, and limiting sleeves 304 are arranged on the opposite outer sides of the two fixing seats 303, and the limiting sleeves 304 are arranged on the opposite outer sides of the fixing seats 303. The positioning sleeve 304 is slidably mounted on the limiting round rod 105. Through the cooperation between the lifting and pressing plates 202 of the culture plate tube 2 and the first compression spring 103, the lifting and pressing plates 202 on the front and rear sides can be manually pressed to make the culture plate tube 2 slide downward along the limiting groove 102, so that the docking sleeve 203 can be detached from the bottom end of the docking column 502. At the same time, the limiting sleeve 304 and the limiting round rod 105 are slidably cooperated to control the mounting frame 3 to drive the chromatography column body 4 to slide left and right. By adjusting the position of the chromatography column body 4, the use of all culture plate tubes 2 in this device can be met.

[0039] Embodiment 2, based on embodiment 1, as Figure 6 As shown, a connector 401 is provided at the bottom end of the chromatography column body 4, a sliding sleeve 402 is provided at the center of the connector 401, and the liquid outlet pipe 5 is slidably connected to the connector 401 through the sliding sleeve 402;

[0040] A connecting plate 501 is provided between the eight liquid outlet pipes 5, and docking posts 502 are provided at the front and rear ends of the connecting plate 501, and the docking posts 502 match the docking sleeve 203, and a second compression spring 503 is sleeved on the liquid outlet pipe 5, and the second compression spring 503 is supported between the connecting plate 501 and the connector 401;

[0041] A sealing ring 504 is provided at the top of the liquid outlet pipe 5, and the sealing ring 504 matches the top of the sliding sleeve 402. A liquid inlet 505 is provided on the side wall of the liquid outlet pipe 5 near the top. By lifting the lifting plate 202 of the culture plate discharge pipe 2 upwards and utilizing the cooperation between the docking sleeve 203 and the docking column 502, the connecting plate 501 can drive the liquid outlet pipe 5 to slide upwards. When the liquid inlet 505 is separated from the sliding sleeve 402, the chromatography liquid in the chromatography column body 4 drips into the culture plate discharge pipe 2 through the liquid inlet 505 and the liquid outlet pipe 5. The operation process is simple and the opening and closing of the chromatography column body 4 at eight locations can be controlled simultaneously.

[0042] The working principle of this embodiment: when in use, the lifting and pressing plates 202 of the culture plate tube 2 cooperate with the first compression spring 103, and the front and rear lifting and pressing plates 202 can be manually pressed to make the culture plate tube 2 slide downward along the limiting groove 102, so that the docking sleeve 203 can be detached from the bottom end of the docking column 502, and at the same time, the limiting sleeve 304 and the limiting round rod 105 are slidably cooperated to control the mounting frame 3 to drive the chromatography column body 4 to slide left and right, and the position of the chromatography column body 4 is adjusted to meet the use of all the culture plate tubes 2 in this device; by lifting the lifting and pressing plates 202 of the culture plate tube 2 upward, and using the docking sleeve 203 and the docking column 502 to cooperate with each other, the connecting plate 501 can drive the liquid outlet pipe 5 to slide upward, and when the liquid inlet 505 is detached from the sliding sleeve 402, the chromatographic liquid in the chromatography column body 4 drips into the culture plate tube 2 through the liquid inlet 505 and the liquid outlet pipe 5, and the operation process is simple, and the opening and closing of eight chromatography column bodies 4 can be controlled at the same time.

[0043] The above are only specific embodiments of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present disclosure, which should be included in the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be based on the protection scope of the claims.

Claims

1. A chromatography column for a cell culture plate, characterized in that: include: A culture plate base, wherein a culture plate pipe is arranged on the top of the culture plate base, and the culture plate pipe is distributed in a linear array; a mounting frame is arranged above the culture plate base; eight chromatography column bodies are arranged on the mounting frame, and the eight chromatography column bodies are distributed in a linear array; a liquid outlet pipe is arranged at the bottom of the chromatography column body.

2. A chromatography column for a cell culture plate according to claim 1, characterized in that: The culture plate base is a rectangular cavity structure, and a partition is provided in the cavity of the culture plate base, the partition is distributed longitudinally and transversely, and a limiting groove is provided on the transverse partition, and a first compression spring is provided on the front and rear sides of the cavity of the culture plate base, and the front and rear first compression springs are distributed in a symmetrical manner.

3. A chromatography column for a cell culture plate according to claim 2, characterized in that: Support frames are arranged on the left and right sides of the top of the culture plate base, and the two support frames are distributed in a symmetrical manner. Two limiting round rods are arranged between the two culture plate bases, and the two limiting round rods are distributed in a front-to-back parallel manner.

4. A chromatography column for a cell culture plate according to claim 1, characterized in that: The culture plate tube arrangement is composed of eight column tubes, and a connecting block is provided between every two adjacent column tubes, and the culture plate tube arrangement is slidably connected to the limiting groove of the culture plate base through the connecting block, and lifting plates are provided on the front and rear sides of the culture plate tube arrangement, and the first compression spring is supported at the bottom of the lifting plate, and a docking sleeve is provided on the top of the lifting plate.

5. A chromatography column for a cell culture plate according to claim 1, characterized in that: The mounting frame comprises a connecting splint, a connecting rod, a fixing seat and a limiting sleeve. The connecting splint is composed of two groups and is symmetrically distributed in an upper and lower manner. The chromatography column body is clamped between the upper and lower groups of connecting splints and is controlled by locking bolts. Four connecting rods are arranged between the upper and lower groups of connecting splints, and every two connecting rods form a group and are symmetrically distributed in a left-right manner. A fixing seat is arranged in the middle position of each group of connecting rods, and limiting sleeves are arranged on the outer sides of the two fixing seats, and the limiting sleeves are slidably sleeved on the limiting round rods.

6. A chromatography column for a cell culture plate according to claim 1, characterized in that: A connector is provided at the bottom end of the chromatography column body, a sliding sleeve is provided at the center of the connector, and the liquid outlet pipe is slidably connected to the connector through the sliding sleeve.

7. A chromatography column for a cell culture plate according to claim 1, characterized in that: A connecting plate is provided between the eight liquid outlet pipes, and docking posts are provided at the front and rear ends of the connecting plate, and the docking posts match the docking sleeve. A second compression spring is sleeved on the liquid outlet pipe, and the second compression spring is supported between the connecting plate and the connector.

8. A chromatography column for a cell culture plate according to claim 7, characterized in that: The top of the liquid outlet pipe is provided with a sealing ring, and the sealing ring matches the top of the sliding sleeve, and a liquid inlet is provided at a position close to the top of the side wall of the liquid outlet pipe.

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