Drug sensitivity detection equipment for brain glioma organs
By designing a drug sensitivity detection device for organoids of glioma in brain, using mobile mixing components and liquid detectors, the problems of difficulty in transferring sample containers, insufficient contact between drug liquid and organoids and difficulty in detecting chemical components in the prior art are solved, and the rapid and accurate drug sensitivity detection effect is achieved.
Patent Information
- Application Number
- CN202520107484.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-17
- Publication Date
- 2025-02-28
- Estimated Expiration
- 2035-01-17
AI Technical Summary
The prior art is difficult to transfer the sample container to the detection site for testing, it is difficult to achieve sufficient contact between the drug solution and the organoid during testing, and it is difficult to detect and analyze the chemical components in the aqueous solution every time it is processed.
A drug sensitivity detection device including a mobile mixing assembly and a liquid detector is designed to achieve rapid mixing of organoids and reaction liquid through a mobile mixing assembly, and to detect chemical components in the aqueous solution in real time through a liquid detector.
It achieves full contact between the medicinal fluid and the organoid, and can quickly and accurately detect the impact of different reagents on the organoid, solving the shortcomings of detection equipment in the prior art.
Smart Images
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Abstract
Description
Technical Field
[0001] The utility model belongs to the field of drug sensitivity detection equipment, and specifically relates to a drug sensitivity detection equipment for brain glioma organoids. Background Art
[0002] Glioma is the most common malignant tumor of the central nervous system. It has poor treatment effects and high recurrence rates, which seriously threaten the life and health of patients. Traditional drug sensitivity detection methods mainly rely on in vitro cell experiments and animal models. The technology for establishing glioma organoids has made some progress in recent years, but there is still a lack of drug sensitivity detection equipment for glioma organoids. There is a lack of efficient, accurate, and rapid detection equipment that can effectively evaluate the inhibitory effects of different drugs on the growth of glioma organoids.
[0003] At present, doctors usually extract brain glioma tissue samples from patients through surgical resection, puncture biopsy or biopsy guided by magnetic resonance imaging. The extracted tissue samples are placed in a specific culture environment, such as a culture dish, and necessary growth factors and nutrients are added. Under these conditions, the tumor tissue samples will gradually grow into tumor-like organoids. Then, different acidic or alkaline liquids are added to the organoids, and the chemical components in the aqueous solution are detected using liquid detection equipment to determine the effects of different liquids on the organs. The drug sensitivity detection equipment in the prior art has difficulty in transferring the sample container to the detection point for detection, and it is difficult to achieve sufficient contact between the liquid and the organoid during detection, and it is difficult to detect and analyze the chemical components in the aqueous solution during each treatment, which needs further improvement.
[0004] Therefore, a drug sensitivity detection device for brain glioma organoids is proposed, which can move the sample container to the detection point for detection, achieve full contact between the drug solution and the organoid during detection, and detect and analyze the chemical components in the aqueous solution during each treatment. Utility Model Content
[0005] In order to overcome the problems that the drug sensitivity detection equipment in the prior art is difficult to move the sample container to the detection point for detection, it is difficult to achieve sufficient contact between the drug solution and the organoid during detection, and it is difficult to detect and analyze the chemical components in the aqueous solution during each treatment, therefore, a drug sensitivity detection equipment for brain glioma organoids is proposed.
[0006] The technical scheme of the utility model is: a drug sensitivity detection device for brain glioma organoids, comprising a bottom plate; a support platform is fixedly connected to the middle part of the upper end of the bottom plate, support blocks are fixedly connected to the front and rear edges of the upper end of the support platform, and one end of the two support blocks close to each other is commonly fixedly connected to a guide rod, and a movable mixing component is sleeved on the side wall of the guide rod, and a first liquid injection component, a first liquid extraction component, a second liquid extraction component and a second liquid injection component are arranged at the left and right edges of the upper end of the bottom plate, and a liquid detector for detection is arranged on the first liquid extraction component;
[0007] The mobile mixing assembly includes a mobile plate, an upper rotating cylinder, a lower rotating cylinder and a fixed tube; a sleeve block is sleeved on the side wall of the guide rod, a mobile plate is fixedly connected to the lower end of the sleeve block, a limit block is fixedly connected to the upper end of the mobile plate, a mobile frame is placed on the upper end of the mobile plate, front and rear ends of the inner wall of the mobile frame are slidably arranged on the front and rear ends of the limit block, a first rack is fixedly connected to the rear end of the mobile frame, a second rack is fixedly connected to the front end of the mobile frame, two first rotating columns are rotatably installed at the upper and lower ends of the mobile plate, the lower end of the first rotating column is fixedly connected to the upper rotating cylinder, and the upper part of the side wall of the first rotating column is fixedly connected to the upper rotating cylinder. A gear ring is connected, and the gear ring and the first rack are meshed with each other. The lower end of the movable plate is fixedly connected to the first motor, and the upper end of the output shaft of the first motor passes through the movable plate and is fixedly connected to a gear, and the gear and the second rack are meshed with each other. The upper end of the upper rotating cylinder is a closed structure, and the lower rotating cylinder is threadedly installed on the inner wall of the lower end of the upper rotating cylinder. Four evenly distributed fixed blocks are fixedly connected to the side wall of the lower rotating cylinder, and a fixed tube is fixedly connected to the upper end of the fixed block in a through-type manner. The interior of the fixed block is a hollow structure, and four evenly distributed grooves are opened on the inner wall of the lower rotating cylinder, and the interior of the groove and the fixed block are interconnected.
[0008] Preferably, the device is used in a sterile room, the organoids are placed inside the lower rotating cylinder, the reaction liquid is poured into the lower rotating cylinder until the organoids are immersed, and then the upper outer wall of the lower rotating cylinder is manually threaded onto the lower inner wall of the upper rotating cylinder, and then the first motor is turned on to rotate the gear, the gear and the second rack are meshed with each other, and the first rack and the gear ring are meshed with each other, and when the output shaft of the first motor rotates forward or reversely, the two upper rotating cylinders can be easily rotated to a certain extent, thereby achieving rapid mixing of the organoids and the reaction liquid in the lower rotating cylinder, and then different reagents are placed in the first injection component, and the first injection component is turned on to place different reagents into the fixed tube. The reagents will fall into the interior of the lower rotating cylinder and can be mixed with the reaction liquid again, and then the lower rotating cylinder continues to rotate to achieve The reagent is mixed with the organoid contained in the lower rotating cylinder, and then the movable plate is manually moved forward, the first liquid extraction component is opened to extract the mixed liquid and detect it with a liquid detector, and then the mobile mixing component is moved to make the fixed tube move to the lower end of the first liquid injection component, the first liquid injection component is opened to place another reagent into the interior of the fixed tube, and the mixture is mixed again, and the mixture is moved to the first liquid extraction component or the second liquid extraction component again for liquid extraction detection. By repeating the above steps, the effects of various different reagents on the organoids can be quickly obtained, which solves the problems in the prior art that it is difficult to transfer the sample container to the detection point for detection, it is difficult to achieve sufficient contact between the drug solution and the organoid during detection, and it is difficult to detect and analyze the chemical components in the aqueous solution during each treatment.
[0009] Preferably, the lower end of the movable plate is fixedly connected to two fixed seats, rollers are rotatably installed on both sides of the inner wall of the fixed seat, the upper end of the support platform is fixedly connected to a pad, and the upper end of the pad is fixedly connected to two U-shaped blocks, and the left and right ends of the inner wall of the U-shaped block are in fit with the left and right ends of the roller.
[0010] Preferably, the upper end of the fixed tube is located below the upper end surface of the upper rotating cylinder.
[0011] Preferably, the length of the moving frame is greater than the length of the moving plate, and the distance between the left and right ends of the inner wall of the moving frame is twice the length of the limit block.
[0012] Preferably, the first injection assembly includes a first rotating disk and an injection tube, the upper end of the base plate is fixedly connected to the first bracket, the upper end of the first bracket is fixedly connected to the first cylinder, the lower end of the output shaft of the first cylinder is fixedly connected to the first lifting block, the lower end of the output shaft of the first lifting block is fixedly connected to the second motor, the lower end of the output shaft of the second motor is fixedly connected to the second rotating column, the lower end of the second rotating column is fixedly connected to the first rotating disk, the upper and lower ends of the first rotating disk are fixedly connected with evenly distributed injection tubes, and an electromagnetic valve is provided on the injection tube.
[0013] Preferably, the first liquid extraction component includes a syringe, an electric cylinder, a third lifting block, a long rod and a piston; the second bracket is fixedly connected to the left and right edges of the upper end of the base plate, the second bracket is fixedly connected to the second cylinder at the upper end, the lower end of the output shaft of the second cylinder passes through the second bracket and is fixedly connected to the second lifting block, the lower end of the second lifting block is fixedly connected to the third motor, the lower end of the output shaft of the third motor is fixedly connected to the third rotating column, the lower end of the third rotating column is fixedly connected to the second rotating disk, the upper and lower ends of the second rotating disk are fixedly connected with a syringe, the lower end inner wall of the syringe is threadedly mounted with a mounting cylinder, and the lower end of the mounting cylinder is fixedly connected to a liquid extraction needle.
[0014] Preferably, the lower end of the second rotating disk is fixedly connected to an electric cylinder, the upper end of the output shaft of the electric cylinder passes through the second rotating disk and is fixedly connected to the third lifting block, the lower end of the third lifting block is fixedly connected to a long rod, the lower end of the long rod is fixedly connected to a piston, and the side wall of the piston is in contact with the inner wall of the syringe.
[0015] The beneficial effects of the utility model are as follows: by turning on the first motor to make its output shaft rotate forward or reversely, the two upper rotating cylinders can be rotated to a certain extent, so that the organoids and reaction liquid in the lower rotating cylinder can be quickly mixed, and then different reagents are placed in the first liquid injection component, and the first liquid injection component is turned on to place different reagents into the interior of the fixed tube. The reagents will fall into the interior of the lower rotating cylinder and can be mixed with the reaction liquid again, and then the lower rotating cylinder is continued to rotate to achieve mixing of the reagents with the organoids contained in the lower rotating cylinder, and then the movable plate is manually moved forward, and the first liquid extraction component is turned on to extract the mixed liquid and Use a liquid detector to perform the test, then move the mobile mixing component so that the fixed tube moves to the lower end of the first liquid injection component, open the first liquid injection component to place another reagent into the interior of the fixed tube, mix again, and move to the first liquid extraction component or the second liquid extraction component for liquid extraction detection. Repeat the above steps to quickly obtain the effects of various different reagents on organoids, thereby solving the problems in the prior art that it is difficult to transfer the sample container to the detection point for detection, it is difficult to achieve sufficient contact between the drug solution and the organoid during detection, and it is difficult to detect and analyze the chemical components in the aqueous solution during each treatment. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 What is shown is a three-dimensional schematic diagram of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0017] Figure 2 What is shown is a three-dimensional structural schematic diagram of a mobile mixing component of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0018] Figure 3Shown is a schematic diagram of the three-dimensional structure of a lower rotating cylinder of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0019] Figure 4 What is shown is a three-dimensional structural schematic diagram of a moving plate of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0020] Figure 5 Shown is a three-dimensional structural schematic diagram of a U-shaped block of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0021] Figure 6 Shown is a three-dimensional structural schematic diagram of a first liquid injection component of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0022] Figure 7 What is shown is a schematic diagram of the three-dimensional structure of a first liquid extraction component of a drug sensitivity detection device for brain glioma organoids of the utility model;
[0023] Figure 8 Shown is a schematic diagram of the three-dimensional disassembled structure of the first liquid extraction component of a drug sensitivity detection device for brain glioma organoids of the utility model.
[0024] The marks in the attached drawings are: 1, bottom plate; 2, support table; 3, support block; 4, guide rod; 5, mobile mixing assembly; 501, sleeve block; 502, mobile plate; 503, limit block; 504, mobile frame; 505, first rack; 506, second rack; 507, first rotating column; 508, gear ring; 509, upper rotating cylinder; 510, first motor; 511, gear; 512, lower rotating cylinder; 513, fixed block; 514, fixed pipe; 515, tank; 516, fixed seat; 517, roller; 6, first injection assembly; 601, first bracket; 602, first cylinder; 603, first Lifting block; 604, second motor; 605, second rotating column; 606, first rotating disk; 607, injection tube; 608, solenoid valve; 7, first pumping assembly; 701, second bracket; 702, second cylinder; 703, second lifting block; 704, third motor; 705, third rotating column; 706, second rotating disk; 707, syringe; 708, mounting cylinder; 709, pumping needle; 710, electric cylinder; 711, third lifting block; 712, long rod; 713, piston; 8, liquid detector; 9, second pumping assembly; 10, second injection assembly; 11, pad; 12, U-shaped block. DETAILED DESCRIPTION
[0025] The utility model is further described below in conjunction with the accompanying drawings and embodiments.
[0026] See also Figure 1-Figure 8 The utility model provides an embodiment: a drug sensitivity detection device for brain glioma organoids, comprising a bottom plate 1; a support platform 2 is fixedly connected to the middle of the upper end of the bottom plate 1, and support blocks 3 are fixedly connected to the front and rear edges of the upper end of the support platform 2, and a guide rod 4 is fixedly connected to one end of the two support blocks 3 close to each other, and a moving mixing component 5 is sleeved on the side wall of the guide rod 4. A first liquid injection component 6, a first liquid extraction component 7, a second liquid extraction component 9 and a second liquid injection component 10 are arranged at the left and right edges of the upper end of the bottom plate 1, and a liquid detector 8 for detection is arranged on the first liquid extraction component 7;
[0027] The mobile mixing assembly 5 includes a mobile plate 502, an upper rotating cylinder 509, a lower rotating cylinder 512 and a fixed tube 514; a sleeve block 501 is sleeved on the side wall of the guide rod 4, the lower end of the sleeve block 501 is fixedly connected to the mobile plate 502, the upper end of the mobile plate 502 is fixedly connected to the limit block 503, a mobile frame 504 is placed on the upper end of the mobile plate 502, the front and rear ends of the inner wall of the mobile frame 504 are slidably arranged on the front and rear ends of the limit block 503, the rear end of the mobile frame 504 is fixedly connected to the first rack 505, the front end of the mobile frame 504 is fixedly connected to the second rack 506, the upper and lower ends of the mobile plate 502 are rotatably installed with two first rotating columns 507, the lower end of the first rotating column 507 is fixedly connected to the upper rotating cylinder 509, the upper part of the side wall of the first rotating column 507 A gear ring 508 is fixedly connected, and the gear ring 508 and the first rack 505 are meshed with each other. A first motor 510 is fixedly connected to the lower end of the movable plate 502. The upper end of the output shaft of the first motor 510 passes through the movable plate 502 and is fixedly connected to a gear 511. The gear 511 and the second rack 506 are meshed with each other. The upper end of the upper rotating cylinder 509 is a closed structure. The lower end inner wall of the upper rotating cylinder 509 is threadedly mounted with a lower rotating cylinder 512. The side wall of the lower rotating cylinder 512 is fixedly connected with four evenly distributed fixed blocks 513. The upper end of the fixed block 513 is fixedly connected with a fixed tube 514 in a through-type manner. The interior of the fixed block 513 is a hollow structure. The inner wall of the lower rotating cylinder 512 is provided with four evenly distributed grooves 515. The interior of the grooves 515 and the fixed block 513 are interconnected.
[0028] The device is used in a sterile room, and the organoid is placed inside the lower rotating cylinder 512, and the reaction solution is poured into the lower rotating cylinder 512 until the organoid is immersed. Then, the upper outer wall of the lower rotating cylinder 512 is manually threaded onto the lower inner wall of the upper rotating cylinder 509, and then the first motor 510 is turned on to rotate the gear 511, and the gear 511 and the second rack 506 are meshed with each other, and the first rack 505 and the gear ring 508 are meshed with each other. When the output shaft of the first motor 510 rotates forward or reversely, the two upper rotating cylinders 509 can be easily rotated to a certain extent, thereby achieving rapid mixing of the organoid and the reaction solution in the lower rotating cylinder 512, and then different reagents are placed in the first injection component 6, and the first injection component 6 is turned on to place different reagents. After the reagent reaches the interior of the fixed tube 514, it will fall into the interior of the lower rotating cylinder 512 and can be mixed with the reaction liquid again. Then, the lower rotating cylinder 512 is continued to rotate to mix the reagent with the organoid contained in the lower rotating cylinder 512. Then, the movable plate 502 is manually moved forward, and the first liquid extraction component 7 is turned on to extract the mixed liquid and use the liquid detector 8 for detection. Then, the mobile mixing component 5 is moved so that the fixed tube 514 moves to the lower end of the first liquid injection component 6. The first liquid injection component 6 is turned on to place another reagent into the interior of the fixed tube 514, mix again, and move to the first liquid extraction component 7 or the second liquid extraction component 9 for liquid extraction detection. By repeating the above steps, the effects of various different reagents on organoids can be quickly obtained.
[0029] See also Figure 1 , Figure 2 and Figure 5 In this embodiment, the lower end of the movable plate 502 is fixedly connected to two fixed seats 516, and rollers 517 are rotatably installed on both sides of the inner wall of the fixed seat 516. The upper end of the support platform 2 is fixedly connected to a pad 11, and the upper end of the pad 11 is fixedly connected to two U-shaped blocks 12. The left and right ends of the inner wall of the U-shaped block 12 are fitted with the left and right ends of the roller 517. The roller 517 can slide stably along the left and right ends of the inner wall of the U-shaped block 12. Manually pushing the movable plate 502 can facilitate the rapid movement of the movable plate 502.
[0030] See also Figure 1 and Figure 2 In this embodiment, the upper end of the fixed tube 514 is located below the upper end surface of the upper rotating cylinder 509 , and the movable plate 502 will not cause movement interference to the fixed tube 514 .
[0031] See also Figure 1 and Figure 2In this embodiment, the length of the moving frame 504 is greater than the length of the moving plate 502, and the distance between the left and right ends of the inner wall of the moving frame 504 is twice the length of the limit block 503, so that the moving frame 504 can stably slide a short distance along the front and rear ends of the limit block 503, which is convenient for the forward or reverse rotation of the gear ring 508.
[0032] See also Figure 1 and Figure 6 In this embodiment, the first injection component 6 includes a first rotating disk 606 and an injection tube 607. The upper end of the bottom plate 1 is fixedly connected to the first bracket 601, the upper end of the first bracket 601 is fixedly connected to the first cylinder 602, the lower end of the output shaft of the first cylinder 602 is fixedly connected to the first lifting block 603, the lower end of the output shaft of the first lifting block 603 is fixedly connected to the second motor 604, the lower end of the output shaft of the second motor 604 is fixedly connected to the second rotating column 605, the lower end of the second rotating column 605 is fixedly connected to the first rotating disk 606, the upper and lower ends of the first rotating disk 606 are fixedly connected with uniformly distributed injection tubes 607, and the injection tubes 607 are provided with electromagnetic valves 608. Different types of reagents are injected into the interior of multiple injection tubes 607. Opening the electromagnetic valve 608 can release the reagents in the injection tubes 607, so that the reagents fall into the interior of the fixed tube 514, which can participate in the reaction of the organoid.
[0033] See also Figure 1 , Figure 7 and Figure 8 In this embodiment, the first liquid extraction component 7 includes a syringe 707, an electric cylinder 710, a third lifting block 711, a long rod 712 and a piston 713; the left and right edges of the upper end of the bottom plate 1 are fixedly connected to the second bracket 701, the upper end of the second bracket 701 is fixedly connected to the second cylinder 702, the lower end of the output shaft of the second cylinder 702 passes through the second bracket 701 and is fixedly connected to the second lifting block 703, the lower end of the second lifting block 703 is fixedly connected to the third motor 704, the lower end of the output shaft of the third motor 704 is fixedly connected to the third rotating column 705, and the lower end of the third rotating column 705 is fixedly connected to the There is a second rotating disk 706, and a syringe 707 is fixedly connected to the upper and lower ends of the second rotating disk 706. A mounting cylinder 708 is threadedly installed on the inner wall of the lower end of the syringe 707, and a liquid extraction needle 709 is fixedly connected to the lower end of the mounting cylinder 708. The second cylinder 702 is turned on to adjust the height of the second lifting block 703. The third motor 704 is turned on to rotate the second rotating disk 706, thereby facilitating the rapid adjustment of the position of the liquid extraction needle 709, so that the liquid extraction needle 709 can move downward and be inserted into the interior of the fixed tube 514, so as to facilitate the extraction of the mixed liquid in the fixed tube 514 for detection.
[0034] See also Figure 1 and Figure 8In the present embodiment, the lower end of the second rotating disk 706 is fixedly connected to an electric cylinder 710, the upper end of the output shaft of the electric cylinder 710 passes through the second rotating disk 706 and is fixedly connected to a third lifting block 711, the lower end of the third lifting block 711 is fixedly connected to a long rod 712, the lower end of the long rod 712 is fixedly connected to a piston 713, the side wall of the piston 713 is in contact with the inner wall of the syringe 707, when the electric cylinder 710 is turned on to make the third lifting block 711 move upward, the piston 713 will move along the inner wall of the syringe 707, so that negative pressure is formed inside the syringe 707, and the mixed liquid on the outer wall of the extraction needle 709 can be drawn into the extraction needle 709 for temporary storage, so as to facilitate the rapid extraction of the mixed liquid.
[0035] Working principle: The device is used in a sterile room. First, place the organoid inside the lower rotating cylinder 512, pour the reaction solution into the lower rotating cylinder 512 until the organoid is immersed, and then manually thread the upper outer wall of the lower rotating cylinder 512 onto the lower inner wall of the upper rotating cylinder 509;
[0036] Then, the first motor 510 is turned on to rotate the gear 511, the gear 511 and the second rack 506 are meshed with each other, and the first rack 505 and the gear ring 508 are meshed with each other. When the output shaft of the first motor 510 rotates forward or reversely, the two upper rotating cylinders 509 can be rotated to a certain extent to achieve rapid mixing of the organoids and the reaction solution in the lower rotating cylinder 512;
[0037] Next, different reagents are placed in the first injection assembly 6, and different reagents are injected into the multiple injection tubes 607. The solenoid valve 608 is opened to release the reagents in the injection tubes 607, so that the reagents fall into the fixed tube 514 and can participate in the reaction of the organoid.
[0038] Then, the lower rotating cylinder 512 is continued to rotate to mix the reagent with the organoid contained in the lower rotating cylinder 512, and then the movable plate 502 is manually moved forward;
[0039] The second cylinder 702 is turned on to adjust the height of the second lifting block 703, and the third motor 704 is turned on to rotate the second rotating disk 706, so as to facilitate the rapid adjustment of the position of the liquid extraction needle 709, so that the liquid extraction needle 709 moves downward and can be inserted into the interior of the fixed tube 514;
[0040] Then, when the electric cylinder 710 is turned on to move the third lifting block 711 upward, the piston 713 moves along the inner wall of the syringe 707, so that negative pressure is formed inside the syringe 707, and the mixed liquid on the outer wall of the liquid extraction needle 709 can be drawn into the liquid extraction needle 709 for temporary storage, so as to facilitate the rapid extraction of the mixed liquid;
[0041] Afterwards, the mixed liquid in the liquid extraction needle 709 is tested using the liquid detector 8;
[0042] Then move the mobile mixing component 5 so that the fixed tube 514 moves to the lower end of the first injection component 6, open the first injection component 6 and place another reagent into the interior of the fixed tube 514, mix again, and move to the first extraction component 7 or the second extraction component 9 for extraction detection. Repeat the above steps to quickly obtain the effects of multiple different reagents on organoids.
[0043] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the purpose of the present invention.
Claims
1. A drug sensitivity detection device for brain glioma organoids, comprising a bottom plate (1); characterized in that: A support platform (2) is fixedly connected to the middle of the upper end of the bottom plate (1), support blocks (3) are fixedly connected to the front and rear edges of the upper end of the support platform (2), and the ends of the two support blocks (3) close to each other are commonly fixedly connected to a guide rod (4), and the side walls of the guide rod (4) are sleeved with a movable mixing assembly (5). A first liquid injection assembly (6), a first liquid extraction assembly (7), a second liquid extraction assembly (9) and a second liquid injection assembly (10) are arranged at the left and right edges of the upper end of the bottom plate (1), and a liquid detector (8) for detection is arranged on the first liquid extraction assembly (7); The mobile mixing assembly (5) comprises a mobile plate (502), an upper rotating cylinder (509), a lower rotating cylinder (512) and a fixed tube (514); a sleeve block (501) is sleeved on the side wall of the guide rod (4); the lower end of the sleeve block (501) is fixedly connected to the mobile plate (502); the upper end of the mobile plate (502) is fixedly connected to a limit block (503); a mobile frame (504) is placed on the upper end of the mobile plate (502); the front and rear ends of the inner wall of the mobile frame (504) are slidably arranged on the front and rear ends of the limit block (503); the rear end of the mobile frame (504) is fixedly connected to a first rack (505); the front end of the mobile frame (504) is fixedly connected to a second rack (506); the upper and lower ends of the mobile plate (502) are rotatably mounted with two first rotating columns (507); the lower end of the first rotating column (507) is fixedly connected to the upper rotating cylinder (509); the side wall of the first rotating column (507) A gear ring (508) is fixedly connected to the upper portion, and the gear ring (508) and the first rack (505) are meshed with each other. A first motor (510) is fixedly connected to the lower end of the movable plate (502), and the upper end of the output shaft of the first motor (510) passes through the movable plate (502) and is fixedly connected to a gear (511), and the gear (511) and the second rack (506) are meshed with each other. The upper end of the upper rotating cylinder (509) is a closed structure, and a lower rotating cylinder (512) is threadedly mounted on the inner wall of the lower end of the upper rotating cylinder (509). Four evenly distributed fixed blocks (513) are fixedly connected to the side wall of the lower rotating cylinder (512), and a fixed tube (514) is fixedly connected to the upper end of the fixed block (513) in a through-type manner. The interior of the fixed block (513) is a hollow structure, and four evenly distributed grooves (515) are opened on the inner wall of the lower rotating cylinder (512), and the interiors of the grooves (515) and the fixed block (513) are interconnected.
2. The drug sensitivity detection device for brain glioma organoids according to claim 1, characterized in that: The lower end of the movable plate (502) is fixedly connected to two fixed seats (516), and rollers (517) are rotatably mounted on both sides of the inner wall of the fixed seat (516). The upper end of the support platform (2) is fixedly connected to a pad (11), and the upper end of the pad (11) is fixedly connected to two U-shaped blocks (12), and the left and right ends of the inner wall of the U-shaped block (12) are in contact with the left and right ends of the rollers (517).
3. The drug sensitivity detection device for brain glioma organoids according to claim 1, characterized in that: The upper end of the fixed tube (514) is located below the upper end surface of the upper rotating cylinder (509).
4. The drug sensitivity detection device for brain glioma organoids according to claim 1, characterized in that: The length of the moving frame (504) is greater than the length of the moving plate (502), and the distance between the left and right ends of the inner wall of the moving frame (504) is twice the length of the limiting block (503).
5. The drug sensitivity detection device for brain glioma organoids according to claim 1, characterized in that: The first liquid injection assembly (6) comprises a first rotating disk (606) and a liquid injection tube (607); the upper end of the bottom plate (1) is fixedly connected to the first bracket (601); the upper end of the first bracket (601) is fixedly connected to the first cylinder (602); the lower end of the output shaft of the first cylinder (602) is fixedly connected to the first lifting block (603); the lower end of the output shaft of the first lifting block (603) is fixedly connected to the second motor (604); the lower end of the output shaft of the second motor (604) is fixedly connected to the second rotating column (605); the lower end of the second rotating column (605) is fixedly connected to the first rotating disk (606); the upper and lower ends of the first rotating disk (606) are fixedly connected to uniformly distributed liquid injection tubes (607); and the liquid injection tubes (607) are provided with electromagnetic valves (608).
6. The drug sensitivity detection device for brain glioma organoids according to claim 1, characterized in that: The first liquid extraction assembly (7) comprises a syringe (707), an electric cylinder (710), a third lifting block (711), a long rod (712) and a piston (713); the left and right edges of the upper end of the bottom plate (1) are both fixedly connected to a second bracket (701); the upper end of the second bracket (701) is fixedly connected to a second cylinder (702); the lower end of the output shaft of the second cylinder (702) passes through the second bracket (701) and is fixedly connected to a second lifting block (703); the second lifting block ( The lower end of the third motor (704) is fixedly connected to the third motor (704), the lower end of the output shaft of the third motor (704) is fixedly connected to the third rotating column (705), the lower end of the third rotating column (705) is fixedly connected to the second rotating disk (706), the upper and lower ends of the second rotating disk (706) are fixedly connected to the syringe (707) in a through-type manner, the inner wall of the lower end of the syringe (707) is threadedly mounted with a mounting cylinder (708), and the lower end of the mounting cylinder (708) is fixedly connected to a liquid extraction needle (709).
7. The drug sensitivity detection device for brain glioma organoids according to claim 6, characterized in that: The lower end of the second rotating disk (706) is fixedly connected to an electric cylinder (710); the upper end of the output shaft of the electric cylinder (710) passes through the second rotating disk (706) and is fixedly connected to a third lifting block (711); the lower end of the third lifting block (711) is fixedly connected to a long rod (712); the lower end of the long rod (712) is fixedly connected to a piston (713); and the side wall of the piston (713) is in contact with the inner wall of the syringe (707).