Device for measuring activity of cells in cell culture dish

Abstract

The utility model provides a cell viability measuring device in a cell culture dish, which belongs to the technical field of measuring devices and comprises a base, the base is connected with a detection sensor, a humidifier and a display instrument, one end of the detection sensor is provided with a detection head, and the base is connected with a control button, a humidity sensor, a sealing door and a temperature sensor. An electric turntable and a placement table are arranged in the base, an electric heating plate and a culture dish are arranged in the placement table, the placement table is connected with a partition plate, and the culture dish is connected with a connecting ring. The culture dish has the beneficial effects that the culture dish is prevented from being accidentally splashed into other culture dishes to cause pollution through the partition plate; meanwhile, fan blades are driven to rotate in the fixed cylinder while the partition plate rotates, so that air flow is better increased, the diffusion speed of temperature or humidity can be increased during heating or humidifying, efficiency is improved, diffusion uniformity is guaranteed, and detection accuracy is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of measuring devices, in particular to a device for measuring cell viability in a cell culture dish. Background Art

[0002] Cell viability refers to the percentage of live cells in the total cells in a cell population, in which there are always some cells that have died for various reasons. Cell viability can be determined by the aniline black method, in which dead cells are dyed black by a dye, while live cells are not colored.

[0003] In the prior art, in order to maintain an ideal environment for cell growth, the culture dish needs to be placed under constant temperature and humidity conditions. If the environmental control inside the cell viability detector is not accurate, it may affect the growth of cells and the accuracy of the experimental results. Therefore, a temperature and humidity adjustment device is usually provided in the detection device. However, when adjusting the temperature and humidity, the corresponding device is fixedly installed in a certain position, resulting in uneven temperature and humidity diffusion during adjustment, which affects the detection results. How to invent a cell viability measurement device in a cell culture dish to improve these problems has become an urgent problem to be solved by technicians in this field. Utility Model Content

[0004] In order to make up for the above deficiencies, the utility model provides a cell viability determination device in a cell culture dish, which aims to improve the problem that in order to maintain an ideal environment for cell growth, the culture dish needs to be placed under constant temperature and humidity conditions. If the environmental control inside the cell viability detector is not accurate, it may affect the growth of cells and the accuracy of the experimental results. Therefore, a temperature and humidity adjustment device is usually provided in the detection device. However, when adjusting the temperature and humidity, the corresponding device is fixedly installed in a certain position, which makes the temperature and humidity diffuse unevenly during the adjustment, affecting the detection results.

[0005] The utility model is achieved in this way:

[0006] The utility model provides a device for measuring cell viability in a cell culture dish, comprising a base, the base is connected with a detection sensor, a humidifier and a display instrument, one end of the detection sensor is provided with a detection head, the base is connected with a control button, a humidity sensor, a sealing door and a temperature sensor, a cavity is provided inside the base, an electric turntable and a placing table are provided inside the base, an electric heating plate and a culture dish are provided inside the placing table, the placing table is connected with a partition plate, a fixing cylinder and a fan blade are provided inside the partition plate, and the culture dish is connected with a connecting ring.

[0007] Preferably, the base is fixedly connected to the detection sensor, the humidifier and the display respectively, one end of the detection sensor is fixedly connected to the detection head inside the cavity, the detection sensor is connected to a transmission wire, the detection sensor and the display are connected by a transmission wire signal, the base is rotatably connected to the sealing door, and the humidifier is fixedly connected to a dispersion tube at one end located inside the cavity.

[0008] By adopting the above technical solution, the water can be better atomized and dispersed through the dispersion pipe, thereby increasing the humidity adjustment speed.

[0009] Preferably, the base is fixedly connected to the control button, the humidity sensor and the temperature sensor, the humidity sensor and the temperature sensor are electrically connected to the display, and the control button is electrically connected to the detection sensor, the humidifier, the display, the detection head, the humidity sensor, the temperature sensor, the electric turntable and the electric heating plate.

[0010] Preferably, the electric turntable is fixedly connected to the inner wall of the bottom end of the cavity, the rotating end of the electric turntable is fixedly connected to the placement table, the upper end of the placement table is fixedly connected to the partition plate, the side wall of the partition plate is fixedly connected to the fixed cylinder, and the interior of the fixed cylinder is rotatably connected to the fan blades.

[0011] By adopting the above technical solution, the partition plate can better separate the culture dishes to prevent the culture dishes from splashing into other culture dishes and causing contamination due to accidental splashing. While rotating, the fan blades are driven to rotate inside the fixed cylinder, thereby better increasing the air flow, so that the diffusion rate of temperature or humidity can be increased during heating or humidification, thereby improving efficiency.

[0012] Preferably, the placement table is provided with three placement grooves distributed in a circumferential array on the side wall separated by the partition plate.

[0013] Preferably, the inner wall of the placement groove away from the opening is fixedly connected to the electric heating plate, the placement groove is connected to the culture dish, the opening end of the culture dish is connected to the connecting ring, and the outer wall of the connecting ring has a frosted texture.

[0014] By adopting the above technical solution, the placement groove can restrict the culture dish to prevent the culture dish from falling off due to centrifugal force when the electric turntable rotates. In addition, the temperature of the culture dish can be adjusted by the electric heating plate.

[0015] The beneficial effects of the utility model are:

[0016] The water inside the humidifier can be better atomized and dispersed through the dispersion tube to improve the humidity adjustment speed, and the temperature can be transmitted to the display instrument for observation in time through the humidity sensor and the temperature sensor, so as to maintain an ideal environment for cell growth and ensure the accuracy of cell growth and experimental results. In addition, the sealing door can ensure the sealing effect of the cavity inside the base, reduce the pollution caused by the long-term exposure of the culture dish to the air, so as to improve the detection result of the detection head; the partition plate can better separate the culture dish to prevent the culture dish from splashing into other culture dishes when it is accidentally splashed; and the culture dish is restricted by the placement groove to prevent the culture dish from falling off due to centrifugal force when the electric turntable rotates. At the same time, the fan blades are driven to rotate inside the fixed cylinder to better increase the air flow, so as to increase the diffusion speed of temperature or humidity during heating or humidification, improve efficiency, ensure the uniformity of diffusion, and improve detection accuracy; the temperature of the culture dish is adjusted by the electric heating plate to control the accuracy of the ambient temperature, and the matte texture connection ring can ensure the taking of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a schematic diagram of the structure of the detection device provided by the embodiment of the utility model;

[0019] Figure 2 It is a schematic diagram of the internal structure of the detection device provided by the embodiment of the utility model;

[0020] Figure 3 The utility model embodiment provides Figure 2 A schematic diagram of the structure at center

[0021] Figure 4 It is a schematic diagram of the partial explosion structure of the placement table provided in the embodiment of the utility model.

[0022] In the figure: 1. base; 2. detection sensor; 3. humidifier; 4. transmission wire; 5. display; 6. control button; 7. humidity sensor; 8. sealing door; 9. temperature sensor; 10. dispersion tube; 11. detection head; 12. placement table; 13. fixing cylinder; 14. partition plate; 15. electric heating plate; 16. culture dish; 17. connecting ring; 18. electric turntable; 19. placement slot; 20. fan blade. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the implementation of the utility model clearer, the technical solution in the implementation of the utility model will be clearly and completely described below in conjunction with the drawings in the implementation of the utility model. Obviously, the described implementation is a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Example

[0025] Reference Figure 1-Figure 4 A device for measuring cell viability in a cell culture dish comprises a base 1, the base 1 is connected with a detection sensor 2, a humidifier 3 and a display 5, one end of the detection sensor 2 is provided with a detection head 11, the base 1 is connected with a control button 6, a humidity sensor 7, a sealing door 8 and a temperature sensor 9, a cavity is provided inside the base 1, an electric turntable 18 and a placement table 12 are provided inside the base 1, an electric heating plate 15 and a culture dish 16 are provided inside the placement table 12, a partition plate 14 is connected to the placement table 12, a fixing cylinder 13 and a fan blade 20 are provided inside the partition plate 14, and a connecting ring 17 is connected to the culture dish 16.

[0026] Furthermore; the base 1 is fixedly connected to the detection sensor 2, the humidifier 3 and the display 5 respectively, one end of the detection sensor 2 is fixedly connected to the detection head 11 inside the cavity, the detection sensor 2 is connected to the transmission wire 4, the detection sensor 2 and the display 5 are connected by signal through the transmission wire 4, the base 1 is rotatably connected to the sealing door 8, and the end of the humidifier 3 located inside the cavity is fixedly connected to the dispersion tube 10; the base 1 is fixedly connected to the control button 6, the humidity sensor 7 and the temperature sensor 9, the humidity sensor 7 and the temperature sensor 9 are connected to the display 5 by electrical signals, and the control button 6 is electrically connected to the detection sensor 2, the humidifier 3, the display 5, the detection head 11, the humidity sensor 7, the temperature sensor 9, the electric turntable 18 and the electric heating plate 15.

[0027] It should be noted that the moisture inside the humidifier 3 can be better atomized and dispersed through the dispersion tube 10, the humidity adjustment speed can be improved, and the temperature can be timely transmitted to the display instrument 5 for observation through the humidity sensor 7 and the temperature sensor 9, so as to maintain an ideal environment for cell growth and ensure the accuracy of cell growth and experimental results. In addition, the sealing door 8 can ensure the sealing effect of the internal cavity of the base 1, reduce the pollution caused by the long-term exposure of the culture dish 16 to the air, so as to improve the detection result of the detection head 11.

[0028] Reference Figure 2-Figure 4, further; the electric turntable 18 is fixedly connected to the inner wall of the bottom end of the cavity, the rotating end of the electric turntable 18 is fixedly connected to the placement table 12, the upper end of the placement table 12 is fixedly connected to the partition plate 14, the side wall of the partition plate 14 is fixedly connected to the fixed cylinder 13, and the interior of the fixed cylinder 13 is rotatably connected to the fan blade 20; the placement table 12 is provided with three placement grooves 19 distributed in a circumferential array on the side wall separated by the partition plate 14; the inner wall of the placement groove 19 away from the opening is fixedly connected to the electric heating plate 15, the placement groove 19 is connected to the culture dish 16, the opening end of the culture dish 16 is connected to the connecting ring 17, and the outer wall of the connecting ring 17 is frosted.

[0029] It should be noted that: the partition plate 14 can better separate the culture dishes 16 to prevent the culture dishes 16 from splashing into other culture dishes 16 and causing contamination when accidentally splashed; in addition, the placement groove 19 restricts the culture dishes 16 to prevent the culture dishes 16 from falling off due to centrifugal force when the electric turntable 18 rotates. While rotating, the fan blades 20 are driven to rotate inside the fixed cylinder 13, thereby better increasing the air flow, so that the diffusion speed of temperature or humidity can be increased during heating or humidification, thereby improving efficiency; and the temperature of the culture dishes 16 is adjusted by the electric heating plate 15 to control the accuracy of the ambient temperature. In addition, the frosted connecting ring 17 can ensure the taking of the culture dishes 16.

[0030] The working principle of this method is as follows: during detection, the culture dish 16 is placed in the placement slot 19, and the sealing door 8 is closed and the electric turntable 18 is rotated so that one of the culture dishes 16 is located directly below the detection head 11, and the detection is performed one by one. The water inside the humidifier 3 can be better atomized and dispersed through the dispersion tube 10, the humidity adjustment speed can be improved, and the temperature can be timely transmitted to the display instrument 5 for observation through the humidity sensor 7 and the temperature sensor 9, so as to maintain an ideal environment for cell growth and ensure the accuracy of cell growth and experimental results. In addition, the sealing door 8 can ensure the sealing effect of the internal cavity of the base 1, reduce the pollution caused by the long-term exposure of the culture dish 16 to the air, so as to improve the detection result when the detection head 11 detects; The partition plate 14 can better separate the culture dishes 16 to prevent the culture dishes 16 from splashing into other culture dishes 16 and causing contamination when accidentally splashed; in addition, the placement groove 19 restricts the culture dishes 16 to prevent the culture dishes 16 from falling off due to centrifugal force when the electric turntable 18 rotates. While rotating, the fan blades 20 are driven to rotate inside the fixed cylinder 13, thereby better increasing the air flow, so that the diffusion speed of temperature or humidity can be increased during heating or humidification, thereby improving efficiency; and the temperature of the culture dishes 16 is adjusted by the electric heating plate 15 to control the accuracy of the ambient temperature. In addition, the frosted connecting ring 17 can ensure the taking of the culture dishes 16.

[0031] It should be noted that the specific model and specifications of the motor need to be selected and determined based on the actual specifications of the device, and the specific selection calculation method adopts the existing technology in this field, so it will not be described in detail.

[0032] The above description is only the preferred implementation of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A device for measuring cell viability in a cell culture dish, comprising a base (1), characterized in that: The base (1) is connected to a detection sensor (2), a humidifier (3) and a display (5); one end of the detection sensor (2) is provided with a detection head (11); the base (1) is connected to a control button (6), a humidity sensor (7), a sealing door (8) and a temperature sensor (9); a cavity is provided inside the base (1); an electric turntable (18) and a placement table (12) are provided inside the base (1); an electric heating plate (15) and a culture dish (16) are provided inside the placement table (12); the placement table (12) is connected to a partition plate (14); a fixing cylinder (13) and a fan blade (20) are provided inside the partition plate (14); and the culture dish (16) is connected to a connecting ring (17).

2. The device for measuring cell viability in a cell culture dish according to claim 1, characterized in that: The base (1) is fixedly connected to the detection sensor (2), the humidifier (3) and the display (5) respectively; one end of the detection sensor (2) is fixedly connected to the detection head (11) inside the cavity; the detection sensor (2) is connected to a transmission wire (4); the detection sensor (2) and the display (5) are connected by signals via the transmission wire (4); the base (1) is rotatably connected to the sealing door (8); and one end of the humidifier (3) located inside the cavity is fixedly connected to a dispersion tube (10).

3. The device for measuring cell viability in a cell culture dish according to claim 1, characterized in that: The base (1) is fixedly connected to the control button (6), the humidity sensor (7) and the temperature sensor (9); the humidity sensor (7) and the temperature sensor (9) are electrically connected to the display (5); the control button (6) is electrically connected to the detection sensor (2), the humidifier (3), the display (5), the detection head (11), the humidity sensor (7), the temperature sensor (9), the electric turntable (18) and the electric heating plate (15).

4. The device for measuring cell viability in a cell culture dish according to claim 1, characterized in that: The electric turntable (18) is fixedly connected to the inner wall of the bottom end of the cavity, the rotating end of the electric turntable (18) is fixedly connected to the placement table (12), the upper end of the placement table (12) is fixedly connected to the partition plate (14), the side wall of the partition plate (14) is fixedly connected to the fixed tube (13), and the interior of the fixed tube (13) is rotatably connected to the fan blade (20).

5. The device for measuring cell viability in a cell culture dish according to claim 1, characterized in that: The placement platform (12) is provided with three placement grooves (19) distributed in a circumferential array on the side wall separated by the partition plate (14).

6. The device for measuring cell viability in a cell culture dish according to claim 5, characterized in that: The inner wall of the placement groove (19) away from the opening is fixedly connected to the electric heating plate (15), the placement groove (19) is connected to the culture dish (16), the opening end of the culture dish (16) is connected to the connecting ring (17), and the outer wall of the connecting ring (17) has a frosted texture.

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